KR102312451B1 - Peanut sprout cultivation method using abeliophyllum distichum extract - Google Patents

Abstract

Disclosed is a method for growing peanut sprouts using the Miseon tree extract. In the cultivation method of the peanut sprout of the present invention, after the peanut sprout sprouts in a cultivation machine in a cultivation room maintained at a temperature of 25-28° C. and a humidity of 80% or more, it is cultivated with a diluted solution containing an extract of Miseon tree.

Description

Peanut sprout cultivation method using Miseon tree extract {PEANUT SPROUT CULTIVATION METHOD USING ABELIOPHYLLUM DISTICHUM EXTRACT}

The present invention relates to a method of growing peanut sprouts, and more particularly, to a method of growing peanut sprouts using an extract of Miseon tree, which is known to have excellent biochemical effects such as antioxidant, anticancer, and anti-inflammatory.

2. Description of the Related Art In general, soybeans contain a lot of nutrients such as protein, so it is an important kind of food consumed by humans.

Among the above-mentioned beans, peanut is an annual herbaceous plant of the genus Arachis of the legume family. It grows well at high temperatures and is produced all over the world including Korea and China. It contains a large amount of nutrients such as protein, fat, vitamins, and minerals, It is known that resveratrol is contained, and the resveratrol is contained in red grapes and cucumbers in addition to peanuts and has been reported to have various pharmacological effects.

Resveratrol as described above was first separated and purified from the root of Hellebore around 1940, and in 1992, resveratrol contained in wine became known to have a good effect on cardiovascular diseases.

However, since the content of resveratrol contained in peanuts is very small, it is difficult to expect the effect of resveratrol only by ingestion of peanuts.

On the other hand, resveratrol is a polyphenol-based antioxidant that plants produce to protect themselves when faced with external invasions such as fungi or ultraviolet rays.

By the way, the country of origin is the Andean region of South America, and the peanuts, which are rich in fat and protein, and contain a lot of vitamins and minerals, face the germination process and when germination proceeds, the fat and calories are lowered, and the peanut itself contains Vitamin C, which was not present, is produced, while anticancer and longevity substances such as resveratrol, which were present in trace amounts in peanuts, increase in large quantities.

Peanut sprouts germinated as described above contain much more aspartic acid than bean sprouts in addition to the increase in resveratrol, which is known to be effective for hangover relief.

In addition, peanut sprouts contain about 50% of fat. Most of them are unsaturated fatty acids such as oleic acid and arachitoic acid, which are essential fatty acids, which lower cholesterol levels and prevent heart disease and vascular diseases. It is known to be effective in prolonging sleep and dieting.

Regarding the above-mentioned peanut sprouts, the peanuts before sprouting contain about 2.3~4.5㎍/g of resveratrol depending on the variety, but after germination, it is amplified to 11.7~25.7㎍/g, and recently cultivated in a special way One peanut sprout is also known to contain up to 580 μg/g of resveratrol.

Therefore, as interest in peanut sprouts having excellent and effective conditions as a food as described above increases, a lot of research and development are being made on various methods of use or cultivation methods of peanut sprouts.

For example, Patent Application No. 2015-0078728 discloses a hangover relieving beverage composition containing peanut sprout extract and a manufacturing method thereof, and Patent Application No. 2015-0188318 discloses a beverage containing peanut sprout extract and a manufacturing method thereof. Application No. 2015-0136121 discloses a method for producing soju containing peanut sprout extract, and Patent Application No. 2014-0028134 discloses a seasoning using peanut sprout and a manufacturing method thereof.

In addition, Patent Application No. 2015-0130537 discloses a manufacturing method of yogurt containing peanut sprout extract, and Patent Application No. 2017-0162626 discloses a hangover relieving composition containing fermented ginseng as an active ingredient, Patent Application No. 2016- In No. 0146059, a pharmaceutical composition for preventing or treating osteoporosis containing soya saponin isolated from sprouted peanut extract, patent application No. 2016-0029006, a method for manufacturing a health functional food using sprouted peanut, and patent application No. 2013-0151107 Various products using peanut sprouts are being developed, such as by disclosing food additives with a calorie restriction mimic effect.

On the other hand, in order to cultivate peanut sprouts applied to various fields as described above, water is generally added and used, and in order to increase the efficiency of most sprout cultivation methods, water is recycled and used. Depending on the external environment or the conditions of the growing season, the water used may be altered, which greatly affects the yield.

In addition, in addition to the efficacy of the simple peanut sprout, many more effective substances are contained in the peanut sprout to expand the scope of use of the peanut sprout, or development of a mass production method is also being tried a lot.

For example, Patent Application No. 2015-0007556 discloses a method and apparatus for growing peanut sprouts, Patent Application No. 2015-0007557 discloses Kyobo material for growing peanut sprouts, and Patent Application No. 2015-0081758 discloses a peanut grower are doing

On the other hand, in Patent Publication No. 10-2016-0043843, a method for growing peanut sprouts which is intended to improve the growth environment to suppress the occurrence of diseases and mold, thereby securing high yield and improving texture, and increasing the component content of resveratrol, and this Disclosed are peanut sprouts grown by

In addition, Patent Registration No. 10-1651870 discloses a cultivation method of sprouted peanuts containing a large amount of active ingredients as an eco-friendly cultivation technology that can suppress viruses while maintaining a cultivation environment that maximizes the active ingredients of sprouted peanuts, In the above patent, the invention is fundamentally different from the present invention because bamboo medium is used and bamboo leaves are used as bamboo.

The present invention uses the extract of Miseon tree, which is known to be excellent in biochemical performance such as antioxidant, anticancer, and anti-inflammatory, in the cultivation of peanut sprouts, unlike the above prior art, to increase the component content of resveratrol in the peanut itself, and at the same time to increase the biochemical performance. It is an object of the present invention to provide a method for growing peanut sprouts using the extract of Miseon tree, which can be grown easily by increasing the utilization of peanut sprouts by containing excellent substances in sprouted peanuts.

The present invention in order to achieve the object of the present invention as described above,

Put the peeled peanuts in a growing machine with water and germinate for 3 days. Water is sprayed once every 2 hours for 5 minutes. Pick out the peanuts that do not sprout after 3 days of cultivation, drain all the water in the cultivation period, fill with new water, and spray water diluted with the extract of Miseon tree extract for 5 minutes once every 2 hours, starting 9~ from the first day. It provides a method of growing peanut sprouts using a Miseon tree extract, which is harvested after growing for 10 days.

In the above, it is preferable that the diluted water is 20:1 by volume of the water: Miseon tree extract.

The harvested peanut sprouts are preferably dried for 24 hours at about 55° C. to be dried with far-infrared rays after washing.

The manufacturing method of the present invention can grow peanut sprouts simply and easily, and the peanut sprouts grown by the production method of the present invention have a higher resveratrol content than the peanut sprouts grown by the conventional method, and have antioxidative as well as antibacterial effects. It also has a high content of plant phenolic compounds known to be known, so it can be used in various ways as general vegetables, fresh vegetables, processed foods, functional foods, or raw materials for pharmaceuticals.

1 is a photograph of the growth process of peanut sprouts grown by the method of the present invention.
Figure 2 is a photograph of dried peanut sprouts grown by the present invention.
Figure 3 is a graph of antioxidant activity of peanut sprouts and conventional ones grown by the present invention.
Figure 4 is a graph of the total phenolic compound content of peanut sprouts and conventional ones grown by the present invention.
Figure 5 is a resveratrol content graph of peanut sprouts grown by the present invention and conventional ones.

Hereinafter, the present invention will be described in more detail through examples of the present invention. The following description is provided to help the understanding and practice of the present invention, but is not intended to limit the present invention thereto. It will be understood by those skilled in the art that various changes and modifications can be made within the spirit of the invention as set forth in the following claims.

First, in the peanut sprout cultivation method of the present invention, water is put in a tray-type cultivation machine of the same principle as a conventional bean sprout cultivation Siru, and peanuts are put in to sprout. In the process of germinating peanut sprouts in this way, water is sprayed with a water sprayer installed in the cultivation machine for about 5 minutes at a time about every 2 hours.

It is convenient to use an automatic sprayer to automatically spray water at a predetermined time using a spray hole installed on the upper side of the cultivation room, etc.

The internal temperature in the cultivation machine in the above is set to about 25 ~ 28 ℃, the humidity is maintained at about 80% or more to help the germination and growth of peanut sprouts.

As described above, when the sprouts are grown for 3 days in the cultivation period, peanut sprouts start to come out as shown in FIG.

Then, all the water in the original grower is discarded and filled with fresh water.

Draining and filling in the above is performed using a hole or the like in the cultivation machine, and since this is a general method, a description thereof will be omitted.

Then, the peanut sprouts are grown with a dilution of water and Miseon tree extract in a volume ratio of 20:1. The diluted solution is applied to the peanut sprouts from the top or the side through the sprayer installed in the cultivation room at 2 hour intervals for 5 minutes once. Cultivate peanut sprouts while spraying the diluted solution.

In the above, the ratio of water: Miseon tree extract to 20:1 is because no further effect can be expected when Miseon tree extract exceeds 1, and Miseon tree extract does not matter which method is extracted, and in the present invention, Miseon tree extract Since there is no characteristic of the tree extraction method, a description thereof will be omitted.

Figure 1 b is a peanut sprout cultivation start 6 days, Figure 1 c is 7 days, and Figure 1 d is a photograph of peanut sprouts after 9 days.

As described above, after 9 days of cultivating the peanut sprouts, it is collected, washed, and dried, and dried in a dryer at about 55° C. for 24 hours.

Figure 2 shows that the dried peanut sprouts are packaged.

As described above, the content of the peanut sprouts grown and dried according to the present invention was measured and tested.

[Antioxidant activity test]

The DPPH radical scavenging activity was measured by the Bondet (1997) method for the electron donating ability of each extract to DPPH.

After adding 40 μl of extracts of various concentrations to 760 μl of 300 μM DPPH/EtOH, it was left at 37° C. for 30 minutes, and then absorbance was measured at 515 nm using a UV/Visible spectrophotometer (Berkman, USA).

ABTS radical scavenging activity was measured by the method of Roberty et al. (1999). The ABTS concentration was prepared to be 1,500 at 734 nm, and after reaction with each concentration sample solution at room temperature for 10 minutes, the activity was measured by the difference in absorbance with the control.

Figure 3 is a graph of antioxidant activity of peanut sprouts and conventional ones grown by the present invention. As can be seen in Figure 3, the peanut sprout grown using the extract of the present invention showed that DPPH and ABTS were higher than that of the peanut sprout in the conventional cultivation, indicating that the antioxidant activity of the peanut sprout grown in the present invention is high. can

[Total Phenolic Compound Analysis]

The total phenol content was measured by referring to the Folin-Denis method. After mixing 50 μl of the extract, 950 μl of distilled water, and 500 μl of folin (folin-ciocalteu reagent), 2.5 ml of 20% sodium carbonate was added and reacted at room temperature for 40 minutes. After the reaction, the absorbance of the supernatant was measured at 725 nm using a UV/Visible spectrophotometer, and the amount was converted from a standard curve. As a standard, tannic acid was used and a quantitative linear equation was used.

Figure 4 is a graph of the content of the total phenolic compounds of peanut sprouts and conventional ones grown by the present invention. As can be seen in Figure 4, the peanut sprouts grown using the extract of the present invention can be effectively used for the peanut sprouts because the total phenolic compound content is higher than that of the conventionally cultivated peanut sprouts.

[Analysis of Active Ingredients: Resveratrol Content]

An HPLC system was used to analyze the active ingredient of the extract. Column is Xbridge C18 (250mm×6mm, 5㎛), mobile phase [A:Acetonitile, B:Water (1% acetic acid)] A:B is 1:9 (0-10min), 1:1 (10 -20, gradient), 1:1 (20-30) conditions were analyzed, compared with the resveratrol standard at 310 nm, and a quantitative line using the standard was prepared, and then the content of resveratrol in the sample was quantitatively analyzed.

Figure 5 is a resveratrol content graph of peanut sprouts grown by the present invention and conventional ones. As shown in FIG. 5, the cultivation of peanut sprouts using the extract of chestnut saplings showed a higher resveratrol content than the general cultivation method, so that the use of peanut sprouts could be widely used.

Claims (4)

In the cultivation method of peanut sprout,
Peanut sprout cultivation method using Miseon tree extract, characterized in that after the peanut sprouts sprout in a cultivation machine in the cultivation room where the temperature is 25 ~ 28 ℃ and the humidity is maintained at 80% or more, it is cultivated with a diluted solution containing the Miseon tree extract. The method according to claim 1, wherein the diluent is water and Miseon tree extract in a volume ratio of 20:1. The method according to claim 1, wherein the cultivation with the diluent is characterized in that the diluent is sprayed on the peanut sprouts for 5 minutes at an interval of 2 hours. The method according to claim 1, wherein the cultivation is performed for 9 to 10 days from the first day.

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