KR102308549B1 - Preparation for precooked rice including seaweeds - Google Patents

Preparation for precooked rice including seaweeds Download PDF

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KR102308549B1
KR102308549B1 KR1020190125057A KR20190125057A KR102308549B1 KR 102308549 B1 KR102308549 B1 KR 102308549B1 KR 1020190125057 A KR1020190125057 A KR 1020190125057A KR 20190125057 A KR20190125057 A KR 20190125057A KR 102308549 B1 KR102308549 B1 KR 102308549B1
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seaweed
rice
instant
barley
instant rice
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KR20210042473A (en
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오명철
김수정
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제주국제대학교 산학협력단
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/196Products in which the original granular shape is maintained, e.g. parboiled rice
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/60Edible seaweed
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives

Abstract

본 발명은 백미, 보리, 잡곡 중에서 선택되는 곡류 45 ~ 50 중량%와, 톳, 모자반, 파래, 꼬시래기, 미역, 파래김 중에서 선택되는 해조류 8 ~ 12중량%와, 정제수 40 ~ 45 중량%를 포함하여 구성되는 즉석밥 재료를 준비하고, 각 재료를 세척하여 용기에 투입하여 1차 포장하고, 포장된 즉석밥 재료를 레토르트 투입하여 증숙 및 살균하고, 증숙된 즉석밥 재료를 냉각한 후 상온 건조하여 2차 포장하는 단계를 포함하며, 상기 해조류는 톳과 모자반을 포함하여 적어도 세 종류 이상으로 구성되며 톳보다 모자반의 함량이 더 큰 것을 특징으로 하는 해조류 즉석밥 제조방법을 제공한다.The present invention contains 45 to 50% by weight of grains selected from white rice, barley, and mixed grains, 8 to 12% by weight of seaweeds selected from shiitake, mackerel, seaweed, seaweed, seaweed, and green laver, and 40 to 45% by weight of purified water Prepare the ingredients for instant rice, wash each ingredient, put it in a container, and pack it for the first time Provided is a method for preparing instant seaweed rice, comprising the step of secondary packaging, wherein the seaweed is composed of at least three types, including shiitake and mother-and-a-half, and the content of the seaweed is greater than that of seaweed.

Figure 112019103013259-pat00018
Figure 112019103013259-pat00018

Description

해조류 즉석밥 제조방법 {Preparation for precooked rice including seaweeds}How to make instant seaweed rice {Preparation for precooked rice including seaweeds}

본 발명은 해조류 즉석밥 제조방법에 관한 것으로서, 상세하게는 톳, 모자반 등을 포함하는 해조류와 곡류를 적정한 비율로 제조하여 맛과 풍미, 식감이 뛰어나고 항산화활성이 우수한 새로운 즉석밥을 제안한다.The present invention relates to a method for preparing seaweed instant rice, and more particularly, by preparing seaweed and grains, including seaweed and mackerel, in an appropriate ratio, to provide a novel instant rice with excellent taste, flavor and texture, and excellent antioxidant activity.

해조류는 무기질과 섬유소가 풍부한 저열량식품으로 장운동, 중금속 배출촉진, 고지혈증개선 등 다양한 생리활성이 확인되면서 건강기능성 식품소재로서 많은 관심을 받고 있으며 특히 톳, 모자반, 감태 등의 갈조류는 항암 및 면역활성과 고혈압예방 및 항종양활성이 있는 것으로 알려져 있다.Seaweed is a low-calorie food rich in minerals and fiber, and various physiological activities such as bowel movement, promotion of heavy metal excretion, and improvement of hyperlipidemia have been confirmed. It is known to have antihypertensive and antitumor activity.

연안해역이나 섬지역의 조간대에서 채취되는 해조류는 생산량은 많으나, 대부분 생산시기의 계절적 특성으로 한철에 다량 채취가 이루어지고 주로 건제품 등의 단순가공 외에 부가가치를 제고할만한 다른 수단을 찾기가 어려운 실정이다. 해조류 생산량은 지속적으로 감소 추세에 있는데, 이는 해수온 상승에 의한 생산량 감소에 의한 영향도 있으나 대부분은 노동력 대비 부가가치가 낮아 생산을 기피하고 있기 때문이다. 제주도 등 섬지역에서 많이 채취되는 해조류 중 톳은 품질이 우수하여 생산량의 80% 이상을 외국으로 수출하였으나 최근에는 판로로 감소하였다. Although the production of seaweeds collected from the intertidal zone of coastal waters or island regions is large, most of them are collected in the cold season due to the seasonal characteristics of the production period. . Seaweed production continues to decrease, which is also affected by a decrease in production due to an increase in seawater temperature, but most of them avoid production due to low added value compared to the labor force. Among the seaweeds that are often collected in island areas such as Jeju Island, the quality of chives is excellent, and more than 80% of the production was exported to foreign countries, but recently it has decreased to the market.

최근 여성의 사회진출 증가, 나홀로족(혼족) 증가, 바쁜 경제활동, 간편 지향적 소비자 욕구 등 생활 패턴의 다양한 변화로 장기간 보존이 가능하고 조리 시간이 단축된 편의식품의 수요가 급증하고 있다. 현대 소비자들이 간편성, 고품질, 안전성 등이 강화된 편의식품들이 요구되고 있는 상황에서 해조류의 부가가치를 높이고 어촌 소득증가에 기여하기 위하여 해조류를 활용한 편의식품의 개발 필요성이 제기되고 있다. Recently, the demand for convenience foods that can be preserved for a long time and have a shorter cooking time is rapidly increasing due to various changes in life patterns such as the increase in women's social advancement, the increase in singles (married people), busy economic activities, and convenience-oriented consumer desires. In a situation where modern consumers are demanding convenience foods with enhanced convenience, high quality, and safety, the need for developing convenience foods using seaweed is being raised to increase added value of seaweed and contribute to an increase in income in fishing villages.

해조류로 톳을 이용한 톳밥이 오래전부터 이용되어 왔는데, 밥을 지을 때 뿌려서 함께 익히는 건조 톳 상품도 개발된 바 있으나 톳의 식감, 냄새, 색상 등의 한계로 인하여 소비자들의 선호도를 향상시키지 못하였다. Tobap using seaweed has been used for a long time, and dried shiitake products have been developed, which are sprinkled and cooked together when cooking.

한편, 등록특허 10-1819282호에 따르면, 취나물 또는 톳을 포함하는 즉석 나물 장밥 또는 즉석 나물 장죽 제조 방법이 제안된 바 있는데, 쑥, 설탕 및 쑥 추출물을 혼합하고 숙성시킨 후 여과한 액을 다시 숙성시켜 쑥 발효액을 제조하고, 추가적으로 물에 콩, 다시마 및 표고버섯을 넣고 끓인 후 여과한 추출물에 간장을 넣고 끓인 후, 기제조한 쑥발효액과 혼합하여 숙성시킨 소스를 제조하여 나물 장밥과 함께 취식하는 기술을 제공하고 있다. On the other hand, according to Patent Registration No. 10-1819282, there has been proposed a method for preparing instant vegetable jangbap or instant herb jangjuk containing chwinamul or shiitake mushroom. Mugwort, sugar, and mugwort extract are mixed and aged, and then the filtered solution is aged again. After boiling, add soybean, kelp and shiitake mushrooms to water, add soy sauce to the filtered extract, and boil it. technology is provided.

이러한 종래의 기술은 톳을 포함하는 즉석 식품의 식감, 맛, 풍미의 한계를 벗어나기 어렵기 때문에 부가적으로 쑥발효액과 숙성 소스 등을 필요로 하는 단점이 있었다. 특히 즉석 식품의 제조 과정이 복잡하고 발효액과 숙성 소스의 제조에 과도한 공정이 요구되어 간편식에 부합하는 제조방법으로는 적당하지 않다. This conventional technique has a disadvantage in that it is difficult to escape the limits of texture, taste, and flavor of instant food including shiitake shiitake, so it additionally requires fermented mugwort and aged sauce. In particular, the manufacturing process of instant food is complicated and excessive processes are required for the production of fermented broth and aged sauce, so it is not suitable as a manufacturing method suitable for convenience food.

해조류의 소비 촉진 및 어촌의 소득 증가에 기여하기 위해서는 해조류 고유의 맛과 영양을 살리면서도 현대인의 기호에 부합하고 쉽게 취식이 가능하도록 새로운 해조류 식품 관련 기술개발이 요구되고 있다. In order to promote the consumption of seaweed and contribute to increasing the income of fishing villages, there is a need to develop new seaweed food-related technologies so that it can be easily consumed while maintaining the unique taste and nutrition of seaweed.

본 발명은 전술한 기술적 배경하에서 창안된 것으로, 본 발명의 목적은 해조류를 활용한 고부가가치 식품을 제공하는 것이다. The present invention was created under the above technical background, and an object of the present invention is to provide a high value-added food using seaweed.

본 발명의 다른 목적은 식이섬유 및 폴리페놀 함량이 풍부한 해조류를 이용하여 소비자 선호도가 높은 가공식품을 개발하고 이를 통해 국민 건강 증진에 기여하는 것이다.Another object of the present invention is to develop processed foods with high consumer preference using seaweeds rich in dietary fiber and polyphenols, thereby contributing to the improvement of public health.

본 발명의 또 다른 목적은 해조류와 농산물을 효율적으로 활용하여 부가가치 창출 및 관련 지역과 산업의 소득 증대에 기여하는 것이다.Another object of the present invention is to efficiently utilize seaweed and agricultural products to contribute to the creation of added value and increase in income of related regions and industries.

기타, 본 발명의 또 다른 목적 및 기술적 특징은 이하의 상세한 설명에서 보다 구체적으로 제시될 것이다.In addition, other objects and technical features of the present invention will be presented in more detail in the following detailed description.

상기 목적을 달성하기 위하여, 본 발명은 백미, 보리, 잡곡 중에서 선택되는 곡류 45 ~ 50 중량%와, 톳, 모자반, 파래, 꼬시래기, 미역, 파래김 중에서 선택되는 해조류 8 ~ 12중량%와, 정제수 40 ~ 45 중량%를 포함하여 구성되는 즉석밥 재료를 준비하고, 각 재료를 세척하여 용기에 투입하여 1차 포장하고, 포장된 즉석밥 재료를 레토르트 투입하여 증숙 및 살균하고, 증숙된 즉석밥 재료를 냉각한 후 상온 건조하여 2차 포장하는 단계를 포함하며, 상기 해조류는 톳과 모자반을 포함하여 적어도 세 종류 이상으로 구성되며 톳보다 모자반의 함량이 더 큰 것을 특징으로 하는 해조류 즉석밥 제조방법을 제공한다.In order to achieve the above object, the present invention provides 45 to 50% by weight of grains selected from white rice, barley, and mixed grains, 8 to 12% by weight of seaweeds selected from shiitake, mackerel, green onion, scallop, seaweed, and green laver, and purified water Prepare instant rice ingredients comprising 40 to 45% by weight, wash each ingredient, put it in a container for primary packaging, retort the packaged instant rice ingredients to steam and sterilize, and steam the instant rice ingredients A method for preparing instant seaweed rice, characterized in that the seaweed is composed of at least three or more types, including shiitake and mother-and-a-half, and has a greater content of mother-and-a-half than that of seaweed. to provide.

본 발명에 있어서, 상기 해조류는 용기 투입 전 전처리 과정으로 80 ~ 95℃의 온도에서 2 ~ 10분간 블랜칭을 실시하는 것이 바람직하다.In the present invention, the seaweed is preferably subjected to blanching for 2 to 10 minutes at a temperature of 80 to 95 ℃ as a pretreatment process before input into the container.

본 발명에 있어서, 상기 곡류는 백미에 보리 또는 잡곡이 혼합된 것을 사용할 수 있다. 또한, 본 발명에 있어서, 상기 해조류는 톳과 모자반 및 미역을 포함하며, 톳과 미역은 동일한 비율로 포함되고 모자반은 톳보다 함량이 많게 포함될 수 있다. In the present invention, the grain may be a mixture of white rice and barley or mixed grains. In addition, in the present invention, the seaweed may include shiitake, mother-and-a-half, and seaweed, and may include shikitake and seaweed in the same ratio, and mother-and-a-half may be included in a greater content than that of seaweed.

본 발명에 따르면, 해조류를 활용한 고부가가치 창출을 위한 핵심 기술 확보 및 표준화가 가능하다. 특히 단순가공 해조류의 고부가가치 편의식품으로의 전환을 통한 소비자 이미지를 개선하고 해조류 가공 융복합 기술의 적용을 통한 고부가가치 해조류 가공기술의 발전 가능성 제고한다.According to the present invention, it is possible to secure and standardize core technology for creating high added value using seaweed. In particular, it will improve consumer image by converting simple processed seaweed into high value-added convenience food, and enhance the possibility of developing high value-added seaweed processing technology through application of seaweed processing fusion technology.

본 발명은 또한, 해조류를 활용한 제품 개발 및 상품화가 가능하고 해조류와 농산 원료의 효율적 이용을 통한 부가가치를 창출하여 어가 및 해녀들의 소득 증대에 기여할 수 있다.The present invention also enables product development and commercialization using seaweed, and can contribute to increasing the income of fishermen and haenyeo by creating added value through the efficient use of seaweed and agricultural raw materials.

뿐만 아니라, 본 발명은 식이섬유 및 폴리페놀 함량이 풍부한 해조류 가공식품의 개발을 통한 국민 건강 증진에 기여할 수 있다.In addition, the present invention can contribute to the improvement of public health through the development of processed seaweed food rich in dietary fiber and polyphenol content.

도 1은 본 발명의 해조류 즉석밥 제조 방법의 일례를 보인 공정도1 is a process diagram showing an example of a method for preparing instant seaweed rice according to the present invention;

본 발명은 해조류를 이용한 즉석밥 제조 방법에 관한 것으로, 톳, 모자반 등을 포함하여 가정식 대체식품(Home Meal Replacement : HMR)으로 이용할 수 있는 새로운 즉석 식품을 제안한다. The present invention relates to a method for preparing instant rice using seaweed, and proposes a new instant food that can be used as a home meal replacement (HMR), including H.

본 발명은 해조류 중 영양이 풍부한 톳의 식감을 개선하고 색상 및 냄새를 향상시키기 위해 추가로 모자반과 파래(또는 미역)을 추가하여 소비자의 취식이 용이하도록 하였으며, 즉석 식품에 포함되는 곡류와 해조류의 적정 비율을 제시하여 관능 평가 선호도를 향상시켰다. 본 발명에서 제공하는 해조류 즉석밥은 맛, 식감, 색상 등이 뛰어나고 항산화활성 등의 기능성이 우수하여 현대인의 식사 대용으로 적극 활용될 수 있으며, 관련 어가 및 농가의 부가가치 창출에도 기여할 수 있다. The present invention makes it easier for consumers to eat by adding mung bean and green onion (or seaweed) in order to improve the texture and color and smell of seaweed, which is rich in nutrients. By suggesting an appropriate ratio, the preference for sensory evaluation was improved. Seaweed instant rice provided by the present invention has excellent taste, texture, color, etc., and excellent functions such as antioxidant activity, so it can be actively used as a meal replacement for modern people, and can also contribute to the creation of added value for related fish and farm households.

해조류 seaweed 즉석밥의of instant rice 제조 Produce

본 발명의 즉석밥에 첨가되는 해조류로는 톳, 모자반, 파래, 꼬시래기, 미역, 파래김 중에서 선택되는 둘 이상의 해조를 사용하며, 바람직하게는 톳과 모자반을 포함하여 적어도 세 종류 이상으로 구성되고 톳보다 모자반의 함량을 더 많이 포함하는 것이 좋다. 즉석밥의 곡류로는 백미, 보리, 잡곡 중에서 선택되는 일종 이상, 바람직하게는 이종 이상의 곡물을 사용하며, 상기 잡곡으로는 현미, 찹쌀, 찰흑미, 늘보리, 현미찹쌀, 발아현미, 납작보리, 검정콩(서리태), 붉은팥, 강남콩, 찰수수, 찰기장, 홍미, 흑미, 검정콩(흑태), 녹미, 보리(할맥) 중에서 선택되는 두 종류 이상을 포함할 수 있다. As the seaweed added to the instant rice of the present invention, two or more seaweeds selected from shiitake, mother-of-pearl, green laver, shiraegi, seaweed, and green laver are used. It is better to include more content of mother and child than that. One or more grains selected from white rice, barley, and mixed grains are used as grains for instant rice, and more than one grain is used as the grains. (Seoritae), red bean, gangnam bean, chal sorghum, sticky rice, red rice, black rice, black soybean (heuktae), green rice, and barley (halmaek) may include two or more types.

준비된 재료는 곡류 45 ~ 50 중량%와, 해조류 8 ~ 12중량%와, 정제수 40 ~ 45 중량%를 포함하여 혼합한다. 곡류는 미리 수세 후 탈수하고 수침지 과정을 수행하며, 해조류는 수세 후 탈수하고 절단하여 준비한다.The prepared material is mixed with 45 to 50% by weight of grains, 8 to 12% by weight of seaweed, and 40 to 45% by weight of purified water. Cereals are pre-washed with water and then dehydrated and water immersed, and seaweeds are prepared by dehydration and cutting after washing.

준비된 각 재료는 도 1의 공정도에 예시적으로 나타낸 바와 같이 각 재료 혼합비율을 최적화하여 충진량을 설정하고 진공 포장용기에 투입하여 밀봉한다(1차 포장). 이후 레트르트에 투입하여 증숙 및 살균하고, 증숙된 즉석밥 재료를 냉각한 후 상온 건조하여 2차 포장하는 단계를 거치게 된다. 본 발명에서는 이와 같은 공정 진행 후 2차 포장 전에 즉석밥의 관능평가를 실시하였다. As illustrated in the process diagram of FIG. 1 for each prepared material, the filling amount is set by optimizing the mixing ratio of each material, and it is put into a vacuum packaging container and sealed (primary packaging). After that, it is put into retro, steamed and sterilized, and the steamed instant rice material is cooled, dried at room temperature, and subjected to secondary packaging. In the present invention, sensory evaluation of instant rice was performed before secondary packaging after such a process.

즉석밥에 포함되는 해조류는 진공포장 용기 투입 전 전처리 과정으로 블랜칭을 실시할 수 있고, 곡류는 백미에 보리 또는 잡곡이 혼합된 것을 사용하는 것도 바람직하다. 맛과 풍미, 조직감 등을 고려하여 해조류에 톳과 모자반 및 미역을 사용할 수 있으며, 이 경우 톳과 미역은 동일한 비율로 포함하고 모자반은 톳 보다 많이 함유하는 것이 바람직하다. 이하, 해조류 전처리, 해조류 함량, 곡류 선정 등에 보다 상세하게 설명한다.The seaweed included in the instant rice can be blanched as a pre-treatment process before input into the vacuum packaging container, and it is also preferable to use a mixture of white rice and barley or mixed grains for grains. In consideration of taste, flavor, texture, etc., seaweed can be used in seaweed, and in this case, it is preferable to include shiitake and seaweed in the same proportion, and contain more shiitake and mother-and-a-half than seaweed. Hereinafter, seaweed pretreatment, seaweed content, grain selection, etc. will be described in more detail.

a. 해조류 선정 및 전처리a. Seaweed selection and pretreatment

톳과 같은 해조류에는 중금속 중 비소함량이 높아 해조류 섭취가 건강을 해칠 가능성이 높기 때문에 비소 저감을 위한 방법으로 90℃에서 톳을 블랜칭하였으며, 블랜칭 시간에 따른 비소함량 측정 결과를 표 1에 나타내었다. Since seaweeds such as seaweeds have a high arsenic content among heavy metals, which is highly likely to harm the health of seaweed intake, blanching of seaweed at 90℃ was performed as a method to reduce arsenic. It was.

[표 1] 블랜칭 시간에 따른 비소함량[Table 1] Arsenic content according to blanching time

Figure 112019103013259-pat00001
Figure 112019103013259-pat00001

톳을 90℃에서 블랜칭처리를 할 경우 2분간 처리시 56.6%, 3분처리시 68.3%, 5분처리시 71.7%의 비소함량이 저감되었다. 따라서 톳의 비소함량을 저감하기 위하여 전처리로 블랜칭이 필요하며, 다른 해조류도 즉석밥에 사용하기 위해 미리 전처리 과정으로 80 ~ 95℃의 온도에서 2 ~ 10분간 블랜칭을 실시하는 것이 바람직하다. 해조 즉석밥을 제조하기 위하여 후술하는 본 발명의 실시예에서는 해조류를 90℃에서 5분간 블랜칭 처리하였고, 이후 찬물에 담가 3회 세척한 다음 5cm 크기로 절단한 후 사용하였다.In the case of blanching at 90°C, arsenic content was reduced by 56.6% for 2 minutes, 68.3% for 3 minutes, and 71.7% for 5 minutes. Therefore, blanching is required as a pretreatment to reduce the arsenic content of shiitake, and it is preferable to perform blanching for 2 to 10 minutes at a temperature of 80 to 95° C. In an example of the present invention to be described later to prepare seaweed instant rice, seaweed was blanched at 90° C. for 5 minutes, then soaked in cold water, washed three times, and then cut into 5 cm pieces before use.

톳과 더불어, 다른 해조류로서 모자반, 미역, 꼬시래기, 파래, 파래김 등을 사용하였다. 모자반은 생모자반을 톳의 블랜칭조건과 동일한 조건으로 블랜칭한 후 5cm 크기로 절단한 후 냉동보관하면서 재료로 사용하였다. 미역은 생미역을 톳의 블랜칭조건과 동일한 조건으로 블랜칭한 후 0.3cm × 5cm 크기로 세절한 후 냉동보관하면서 재료로 사용하였다. 꼬시래기는 톳의 블랜칭조건과 동일한 조건으로 블랜칭한 후 5cm 크기로 세절한 후 냉동보관하면서 재료로 사용하였다. 건파래는 상용제품(탐라에이치앤비)을 구입하여 냉동보관하면서 사용하고, 파래김은 상용제품(제주시 농협하나로마트)을 구입하여 일정크기 세절하여 재료로 사용하였다.In addition to shiitake, other seaweeds, such as mackerel, wakame seaweed, shiraegi, green onion, and green laver, were used. For the mother and child, the raw mother and father were blanched under the same conditions as the blanching conditions of hibiscus, cut to a size of 5 cm, and then used as a material while refrigerated. Seaweed was used as a material while blanching raw seaweed under the same conditions as those of shiitake, and then diced into 0.3cm × 5cm pieces and stored frozen. After blanching under the same conditions as the blanching conditions of chives, they were cut into 5 cm pieces, and then used as a material while refrigerated. Dry green seaweed was purchased as a commercial product (Tamra H&B) and used while refrigerated, and green seaweed was purchased as a commercial product (Nonghyup Hanaro Mart, Jeju-si), cut to a certain size, and used as a material.

b. 해조류 첨가량b. seaweed addition

HMR 해조 즉섭밥 제조를 위한 해조류를 선택하고 최적의 배합비율 설정하기 위하여 제주 조간대 해조류 중 생산량이 많으며 기능성이 알려진 톳, 모자반, 및 건파래를 선정하고 톳(1): 모자반(0.8): 건파래(0.2) 의 중량 비율로 혼합한 해조류를 백미와 정제수에 혼합하여 해조즉석밥을 제조하고 관능평가를 실시하였다. 백미 및 정제수, 해조류의 혼합 비율(중량비 %)은 표 2와 같다.Selecting seaweed for HMR seaweed instant rice In order to set the mixing ratio, seaweeds, which are known for their high production and functionality among seaweeds in the intertidal zone of Jeju, were selected and mixed with white rice and dried green onion (1): hat and half (0.8): dried green onion (0.2). Instant seaweed rice was prepared by mixing with purified water, and sensory evaluation was performed. Table 2 shows the mixing ratio (weight ratio %) of white rice, purified water, and seaweed.

[표 2] [Table 2]

Figure 112019103013259-pat00002
Figure 112019103013259-pat00002

관능평가는 9점 척도법으로 색상, 냄새, 맛, 조직감 및 전체적인 기호도에 대하여 ‘매우 좋음’ 9점, 보통 5점, ‘매우 나쁨’1점으로 측정하였고, 그 결과를 표 3에 나타내었다. (실험 결과는 독립적으로 3회 이상 반복 실시하여 평균과 표준편차로 나타내었다. 실험값 사이의 차이는 Duncan의 다중범위검정을 실시하고 각 실험값 사이의 유의적인 차이는 P<0.05 수준에서 검증하였다. 이하의 평가 방법에서도 동일하게 적용하였다.)The sensory evaluation was performed on a 9-point scale, with 9 points of 'very good', 5 points of average, and 1 point of 'very bad' for color, smell, taste, texture, and overall preference, and the results are shown in Table 3. (Experimental results were independently repeated three or more times and expressed as mean and standard deviation. Duncan's multi-range test was performed for differences between experimental values, and a significant difference between each experimental value was verified at P<0.05 level. Below The same was applied to the evaluation method of

[표 3] 해조류 첨가량에 따른 해조류 즉석밥 관능평가[Table 3] Sensory evaluation of seaweed instant rice according to the amount of seaweed added

Figure 112019103013259-pat00003
Figure 112019103013259-pat00003

색상 및 냄새는 전반적으로 낮게 평가되었는데, 해조류 즉석밥의 색상 및 냄새를 고려할 경우 해조류 첨가량을 10% 내외의 범위로 조절할 필요가 있음을 확인하였고, 특히 톳은 해조류 즉석밥의 색상에, 모자반과 파래는 냄새에 영향을 주는 것으로 분석되었다. 맛, 조직감 및 전체적인 기호도는 첨가량에 따른 유의적인 차이는 없었지만 각각 10% 첨가한 시험구에서 가장 높았고 해조류 함량이 높아질수록 조직감이 낮아지는 것은 톳에 기인하는 것으로 확인되었다. 이상의 결과, 해조류 즉석밥 제조시 해조류를 10% 범위 내에서 레시피를 조절하는 것이 바람직하며 해조류 즉석밥의 색상과 조직감을 위하여 톳 함량은 줄이고 모자반 함량을 증가시킬 필요가 있다. The color and smell were evaluated as low overall. Considering the color and smell of the instant rice, it was confirmed that the amount of seaweed should be adjusted within the range of around 10%. was analyzed to affect odor. Although there was no significant difference in taste, texture, and overall preference according to the amount added, it was the highest in the test group with 10% addition, respectively. As a result of the above, it is desirable to adjust the recipe within the range of 10% for seaweed instant rice when preparing seaweed instant rice, and it is necessary to reduce the turmeric content and increase the hat half content for the color and texture of seaweed instant rice.

c. 곡류 선정c. Grain selection

상용 즉석밥에 사용되고 있는 곡류는 백미, 현미, 보리, 혼곡 등이 있으며 본 발명의 실시예에서는 백미, 보리 및 잡곡을 선정하여 해조류 즉석밥을 제조하여 적합한 곡류를 선정하였다. 표 4에 나타난 바와 같이 백미와 보리의 혼합쌀(백미와 보리 7:3 비율), 백미, 보리(찰보리와 쌀보리 1:1 비율), 혼합곡류(농협하나로마트 판매중인 15곡 혼합잡곡)을 대상으로 표 2 관련 해조류를 첨가하여 즉석밥을 제조하고 관능평가를 실시하였다.Grains used for commercial instant rice include white rice, brown rice, barley, mixed grain, and the like. In the embodiment of the present invention, white rice, barley and mixed grains were selected to prepare seaweed instant rice, and suitable grains were selected. As shown in Table 4, mixed rice with white rice and barley (polished rice and barley 7:3 ratio), white rice, barley (stained barley and rice barley 1:1 ratio), and mixed grains (15 mixed and mixed grains sold at Nonghyup Hanaro Mart) are targeted. Table 2 related seaweeds were added to prepare instant rice and sensory evaluation was performed.

[표 4] 곡류 혼합 비율 (중량비 %)[Table 4] Grain mixing ratio (weight ratio %)

Figure 112019103013259-pat00004
Figure 112019103013259-pat00004

관능평가 결과, 표 5에 나타낸 바와 같이 색상은 백미를 사용한 경우가 가장 높았으며 보리 첨가 즉석밥에서 가장 낮았다. 냄새(향)는 곡류의 종류에 따른 차이는 거의 나타나지 않았고, 맛과 조직감은 백미가 가장 높았으며 보리를 사용한 경우낮게 평가되었다. 보리를 사용하고자 할 경우 조직감 등을 개선하기 위하여 물사용량을 증가시키거나 보리를 수침지 후 사용할 필요가 있음을 확인하였다. As a result of sensory evaluation, as shown in Table 5, the color was highest in the case of using white rice and the lowest in the case of instant rice with barley added. There was almost no difference in smell (scent) depending on the type of grain, and the taste and texture were the highest for white rice, and the lowest for barley. In case of using barley, it was confirmed that it is necessary to increase the amount of water used or to use the barley after immersion in order to improve the texture.

[표 5] 곡류에 따른 해조류 즉석밥의 관능평가[Table 5] Sensory evaluation of seaweed instant rice according to grains

Figure 112019103013259-pat00005
Figure 112019103013259-pat00005

d. 모자반 첨가량 설정d. Setting the addition amount of mother and child

톳의 색상과 조직감을 보완하기 위해 해조류에 다른 성분의 첨가량을 증가시킬 필요가 있는데, 모자반은 물성이 부드러우면서 독특한 냄새를 지니고 있어 해조류 즉석밥의 조직감과 향에 긍정적인 영향을 줄 것으로 예상되며, 적절한 혼합 비율을 확인하고자 표 6에 나타난 바와 같이 모자반 첨가량을 달리하여 즉석밥을 제조하고 관능평가를 실시하였다.It is necessary to increase the amount of other ingredients added to seaweed in order to supplement the color and texture of shiitake rice. Since Mozban has soft physical properties and a unique smell, it is expected to have a positive effect on the texture and flavor of instant seaweed rice. , in order to confirm the proper mixing ratio, as shown in Table 6, instant rice was prepared by varying the amount of mother-of-pearl and sensory evaluation was performed.

[표 6] 모자반 첨가량에 따른 해조류 즉석밥 혼합비율 (중량비 %)[Table 6] Mixing ratio of seaweed instant rice according to the addition amount of mother and child (weight ratio %)

Figure 112019103013259-pat00006
Figure 112019103013259-pat00006

관능평가 결과, 표 7에 나타낸 바와 같이 모자반 첨가량이 증가할수록 색상에 대한 선호도가 증가하였고, 향은 해조류의 원초적 냄새인 염분 냄새가 잔존하여 큰 차이를 보이지 않았으며, 맛, 조직감 및 전체적인 기호도는 유의적인 차이는 나타나지 않았지만 D 시험구에서 높게 평가되었다. 종합적으로, 모자반을 5% 이상 첨가할 경우 모든 관능평가 항목에서 기호도가 증가하는 것을 알 수 있고, 해조류 즉석밥 제조시 톳 첨가량을 줄이는 대신 모자반을 톳 보다 많이 첨가할 필요가 있음을 확인하였다. As a result of the sensory evaluation, as shown in Table 7, the preference for color increased as the amount of mother and father added increased, and the smell did not show a significant difference due to the residual salt smell, the original smell of seaweed, and the taste, texture and overall preference were significant. There was no significant difference, but it was highly evaluated in the D test group. Overall, it can be seen that when 5% or more is added, it can be seen that the preference is increased in all sensory evaluation items, and it is confirmed that it is necessary to add more hat and half instead of reducing the amount of hibiscus added when preparing seaweed instant rice.

[표 7] 모자반 첨가량에 따른 해조류 즉석밥 관능평가[Table 7] Sensory evaluation of seaweed instant rice according to the addition amount of mother and child

Figure 112019103013259-pat00007
Figure 112019103013259-pat00007

실시예Example 1 - 1차 해조류 1 - primary seaweed 즉석밥Instant Rice

관능평가 결과 기호도가 가장 높은 조건의 재료들을 대상으로 해조류 즉석밥을 제조하였다. 곡류는 백미를 선정하였고, 해조류로는 톳 2.3%, 모자반 7.1%, 파래 0.1% 의 중량 비율로 첨가하였으며, 상세한 혼합 비율은 표 8과 같다.As a result of sensory evaluation, seaweed instant rice was prepared for the ingredients with the highest preference. White rice was selected as the grain, and seaweed was added in a weight ratio of 2.3% of shiitake, 7.1% of mother-of-pearl, and 0.1% of green seaweed. The detailed mixing ratio is shown in Table 8.

[표 8] 해조류 즉석밥 혼합 비율 (중량비 %)[Table 8] Seaweed instant rice mixing ratio (weight ratio %)

Figure 112019103013259-pat00008
Figure 112019103013259-pat00008

제조된 즉석밥과 대조구로서 톳만을 포함한 즉석밥에 대해 관능평가를 실시한 결과, ㅍ표 9의 결과와 같이 대조구로 사용된 톳밥에 비해 실시예에 따른 해조류 즉석밥은 맛, 조직감 및 전체적인 기호도가 높게 평가되었고, 색상과 냄새항목 평가는 톳밥에 비해 낮게 평가되었지만 유의적인 차이는 없었다. As a result of sensory evaluation of the prepared instant rice and the instant rice containing only Tobbi as a control, as shown in Table 9, the instant rice with seaweed according to the Example was evaluated to have higher taste, texture, and overall preference compared to that used as a control. The color and smell items were evaluated lower than those of tobbap, but there was no significant difference.

[표 9] 1차 즉석밥 관능평가[Table 9] Sensory evaluation of the first instant rice

Figure 112019103013259-pat00009
Figure 112019103013259-pat00009

실시예 1에 따른 해조류 즉석밥은 톳밥에 비해 기호도가 향상된 결과를 얻었지만, 즉석밥 개봉시 뚜겅에 파래가 부착되어 있어 해조류 즉석밥에 대한 이미지가 손상되거나 품질관리에 문제점이 발생할 가능성이 있음을 확인하였다. Although the instant rice with seaweed according to Example 1 obtained a result with improved preference compared to that of rice, it was confirmed that there is a possibility that the image of the instant rice with seaweed may be damaged or there is a problem in quality control because green onions are attached to the lid when the instant rice is opened. did.

해조류 즉석밥의 소비자 선호도가 저하되는 것을 방지할 수 있도록 건조 파래를 대신할 다른 해조류를 모색하기 위하여 제주도 조간대에서 손쉽게 채취가 가능한 꼬시래기, 미역 및 파래김을 선정하였으며 꼬리시래기와 미역은 수분함량을 고려하여 톳첨가량과 동량으로 첨가하여 표 10의 혼합 비율로 즉석밥을 제조하고 관능평가를 실시하였다. In order to prevent a decrease in consumer preference for instant rice with seaweed, in order to search for other seaweeds to replace dried seaweed, we selected seaweed, seaweed and seaweed, which can be easily harvested from the intertidal zone of Jeju Island. Thus, instant rice was prepared at the mixing ratio of Table 10 by adding the same amount as the amount of chives added, and sensory evaluation was performed.

[표 10] 건파래 대체 해조류 즉석밥 혼합비율 (중량비 %)[Table 10] Mixing ratio of instant rice with seaweed substitute for dried green onion (weight ratio %)

Figure 112019103013259-pat00010
Figure 112019103013259-pat00010

관능평가 결과를 표 11에 나타내었다. 파래김 첨가 즉석밥은 색상, 냄새, 맛, 및 조직감 등 모든 관능평가 항목에서 양호하였으나 건조 파래김 제품에 모래 등 이물 존재하였고 압착 건조된 성상으로 인한 절단(세절)작업에 어려움이 존재하였다. 꼬시래기 첨가 즉석밥은 제조 과정에서 높은 열로 인하여 꼬시래기 형상이 사라져 즉석밥의 색상을 탁하게 만들었으며, 냄새, 맛 및 조직감 등에서도 낮게 평가되었다. 미역 첨가 즉석밥은 색상, 냄새, 맛 등 모든 평가항목에서 높은 결과를 보였으며 특히 미역 형태가 남아있어 즉석밥의 해조류 양이 풍성하게 보이는 효과를 얻을 수 있었다. 이상의 결과로부터 해조류 즉석밥 제조시 미역은 파래를 대신할 수 있는 좋은 대체 해조류로 평가되었다.The sensory evaluation results are shown in Table 11. Instant rice with green seaweed added was good in all sensory evaluation items such as color, smell, taste, and texture, but there were foreign substances such as sand in the dried green seaweed product, and there was difficulty in cutting (cutting) work due to the compressed and dried properties. In the instant rice with scallops added, the shape of the scallops disappeared due to high heat during the manufacturing process, making the color of the instant rice cloudy, and the smell, taste, and texture were also evaluated low. Instant rice with seaweed showed high results in all evaluation items such as color, odor, and taste. From the above results, seaweed was evaluated as a good substitute for seaweed when making instant rice with seaweed.

[표 11] 건파래 대체 해조류별 관능평가 결과[Table 11] Results of sensory evaluation by seaweed substitute for dried green onion

Figure 112019103013259-pat00011
Figure 112019103013259-pat00011

실시예Example 2 - 2차 해조류 2 - secondary seaweed 즉석밥Instant Rice

해초밥 개봉시 건조 파래의 문제점 때문에 이를 대신할 수 있는 해조류를 모색한 결과 미역을 첨가할 경우 기호도가 높게 나타남에 따라 미역을 첨가한 혼합비율에 따라 2차 해조 즉석밥을 제조하였다(표 12 혼합비율 참조). As a result of searching for seaweed that can replace seaweed because of the problem of dried green seaweed when opening seaweed rice, the preference was high when seaweed was added. Reference).

백미를 세척하여 용기에 투입하고 해조류로 톳, 모자반, 미역을 정제수와 함께 투입한 후 포장을 거쳐 중량 확인 후 금속검출기를 통과하였다. 포장된 용기를 레토르트에 투입하여 증숙 및 살균을 진행하였다(온도 118℃, 압력 1.73kg/cm3, 시간 40분). 이후 40℃가 될 때까지 20분간 냉각한 후 상온 건조를 거쳐 외포장하였다.After washing the white rice and putting it in a container, seaweed, seaweed, turmeric, and seaweed were added together with purified water, packaged, checked the weight, and then passed through a metal detector. The packaged container was put into a retort, and steaming and sterilization were performed (temperature 118° C., pressure 1.73 kg/cm 3 , time 40 minutes). After cooling for 20 minutes until it reached 40 °C, it was dried at room temperature and then packaged.

[표 12] 해조류 즉석밥 혼합 비율 (중량비 %)[Table 12] Seaweed instant rice mixing ratio (weight ratio %)

Figure 112019103013259-pat00012
Figure 112019103013259-pat00012

해조류로 톳, 모자반 및 미역을 첨가하여 즉석밥을 제조한 후 톳밥을 대조구로 하여 관능평가를 실시한 결과, 표 13에 나타낸 바와 같이 톳밥에 비해 유의적인 차이는 나타나지 않았지만, 맛, 조직감 및 전체적인 기호도가 높게 평가되었다. 특히 백미와 해조류의 혼합으로 씹힘성이 톳밥에 비해 부드러워 거친 맛을 싫어하는 젊은 층에서 선호도 높아질 것으로 기대되었다.As a result, as a result of sensory evaluation using tobap as a control after preparing instant rice by adding seaweed tofu, mackerel, and seaweed, there was no significant difference compared to tobba as shown in Table 13, but the taste, texture and overall preference was highly rated. In particular, it is expected that the preference of young people who do not like the harsh taste will increase because of the combination of white rice and seaweed, which has a softer chew compared to tobbap.

[표 13] 2차 즉석밥 관능평가[Table 13] Sensory evaluation of the second instant rice

Figure 112019103013259-pat00013
Figure 112019103013259-pat00013

해조류 seaweed 즉석밥의of instant rice 항산화 활성 antioxidant activity

항산화 활성 측정용 시료를 준비하였다. 해조류 첨가 즉석밥을 -20℃에서 8시간 이상 냉동 보관한 뒤 믹서기(SMX-8000EMT, Shinil, Seoul, Korea)와 핸드 블랜더(SMX-11HC, Shinil, Seoul, Korea)를 이용하여 균일하게 분쇄하였다. 해조류 밥 분쇄물 5 g에 80% ethanol과 80% methanol을 각각 95 mL 첨가한 뒤, 균질기(HG-15A, Daihan Scientific, Wonju, Korea)를 이용하여 1분간 균질하였다. Shaking incubator(JSSI-100C, JS Research Inc., Gongju, Korea)를 이용하여 상온에서 24시간동안 진탕 추출하였고, 추출물을 Whatman No. 2 여과지로 여과한 후 -20℃에서 냉동 보관하여 시료로 사용하였다.A sample for measuring antioxidant activity was prepared. Instant rice with seaweed was stored frozen at -20°C for more than 8 hours, and then uniformly ground using a blender (SMX-8000EMT, Shinil, Seoul, Korea) and a hand blender (SMX-11HC, Shinil, Seoul, Korea). After adding 95 mL of 80% ethanol and 80% methanol to 5 g of seaweed rice pulverized product, each was homogenized for 1 minute using a homogenizer (HG-15A, Daihan Scientific, Wonju, Korea). Shaking incubator (JSSI-100C, JS Research Inc., Gongju, Korea) was used for extraction with shaking at room temperature for 24 hours, and the extract was subjected to Whatman No. 2 After filtration with filter paper, it was stored frozen at -20°C and used as a sample.

실험 결과는 독립적으로 3회 이상 반복 실시하여 평균과 표준편차로 나타내었다. 모든 실험 결과의 유의성 검증은 SPSS 18.0 (SPSS Inc., Chicago, IL, USA)을 이용하여 ANOVA를 따르고, 실험값 사이의 차이는 Duncan의 다중범위검정을 실시하였다. 각 실험값 사이의 유의적인 차이는 P<0.05 수준에서 검증하였다.Experimental results were independently repeated three or more times and expressed as mean and standard deviation. The significance of all experimental results was verified by ANOVA using SPSS 18.0 (SPSS Inc., Chicago, IL, USA), and Duncan's multi-range test was performed for differences between experimental values. Significant differences between each experimental value were verified at the P<0.05 level.

a. 총폴리페놀 함량a. Total polyphenol content

총폴리페놀 함량은 Folin-ciocalteu 방법을 이용하여 측정하였다. 시료를 5% 농도로 제조한 것에 100 μL와 증류수 1.5 mL 그리고 2 N Folin-ciocalteu’(Sigma-Aldrichm St Louis, Mo, USA) 100 μL를 혼합한 뒤, 30초간 반응시켰다. 20% Sodium carbonate (Na2CO3) (OCI, Inchen, Korea) 300 μL를 가하여 암소에서 1시간 반응 시켰다. 분광광도계 (Optizen, Mecasys Co)를 이용하여 765 nm에서 흡광도를 측정하였다. Gallic acid (Sigma-Aldrich)를 표준물질로 사용하여 동일한 방법으로 작성한 표준곡선으로부터 총 폴리페놀 함량을 밥 또는 즉석밥 1 g 중의 mg gallic acid equivalents (mg GAE/g)으로 나타내었다. L-ascorbic acid (DaeJung chemicals & Metals co., Ltd., Siheung, Korea)은 대조군으로써 10ppm 농도로 제조하여 100 μL 중의 mg gallic acid equivalents (mg GAE/100 μL)으로 나타내었다. The total polyphenol content was measured using the Folin-ciocalteu method. 100 μL of a sample prepared at a concentration of 5%, 1.5 mL of distilled water, and 100 μL of 2 N Folin-ciocalteu' (Sigma-Aldrichm St Louis, Mo, USA) were mixed and reacted for 30 seconds. 300 μL of 20% sodium carbonate (Na2CO3) (OCI, Inchen, Korea) was added and reacted in a dark place for 1 hour. Absorbance was measured at 765 nm using a spectrophotometer (Optizen, Mecasys Co). Total polyphenol content was expressed as mg gallic acid equivalents (mg GAE/g) in 1 g of rice or instant rice from a standard curve prepared in the same way using gallic acid (Sigma-Aldrich) as a standard material. L-ascorbic acid (DaeJung chemicals & Metals co., Ltd., Siheung, Korea) was prepared at a concentration of 10 ppm as a control and expressed as mg gallic acid equivalents (mg GAE/100 μL) in 100 μL.

폴리페놀 화합물은 식물계에 광범위하게 분포되어 있으며 대부분 세포벽 다당류 및 리그닌 등과 에스테르 결합을 이루고 있거나, 중합체로 존재하며 수산기를 통한 수소공여와 페놀 고리구조의 공명 안정화에 의하여 항산화능을 나타내어 체내의 항산화 체계와 함께 자유기로부터 조직을 보호해 주는 역할을 하는 것으로 알려져 있다(Ahn SI 등 2007). 총폴리페놀 함량은 톳 에탄올 추출물에서 26. 27mg GAE/g으로 시험구중 가장 높게 나타났으며 그다음은 미역, 모자반, 파래, 꼬시래기 순으로 확인되었다. Polyphenol compounds are widely distributed in the plant world, and most of them form ester bonds with cell wall polysaccharides and lignin, or exist as polymers. Together, they are known to play a role in protecting tissues from free radicals (Ahn SI et al. 2007). The total polyphenol content was the highest in the test group at 26.27mg GAE/g in the ethanol extract of H. shiitake, followed by seaweed, motherwort, green onion, and mussels in the order.

총폴리페놀 함량은 항산화활성과 밀접한 관계를 가지고 있으며, 해조류 즉석밥의 총폴리페놀함량은 표 14의 결과와 같이 보리와 혼합곡으로 제조한 즉석밥에서 높은 함량을 보였으며 백미만 사용한 즉석밥에서는 그 함량이 낮게 나타났다. 또한 에탄올 추출물이 메탄올추출물에 비해 높은 총폴리페놀함량을 보였다.Total polyphenol content has a close relationship with antioxidant activity. As shown in Table 14, the total polyphenol content of seaweed instant rice was high in instant rice prepared with barley and mixed grains, and in instant rice using only white rice. its content was low. In addition, the ethanol extract showed a higher total polyphenol content than the methanol extract.

[표 14] 해조류 즉석밥의 총폴리페놀 함량[Table 14] Total polyphenol content of seaweed instant rice

Figure 112019103013259-pat00014
Figure 112019103013259-pat00014

b. 환원력b. reducing power

시료 1 mL에 0.2 M phosphate buffer (pH 6.6) 1 mL와 1% potassium ferricyanide 1 mL를 넣고 혼합 후, 50℃에서 20분간 반응시켰다. 10% trichloroacetic acid(TCA) 1 mL 첨가하여 혼합한 후, 반응용액에서 상층액 2 mL에 증류수 2 mL와 0.1 % ferric chloride 400 μL를 혼합하였다. 암소에서 10분간 반응시킨 후, 분광광도계(Optizen 2120UV, Mecasys Co., Daejeon, Korea)를 사용하여 700 nm에서 흡광도를 측정하였다. L-ascorbic acid (DaeJung chemicals & Metals co., Ltd., Siheung, Korea)은 10 ppm 농도로 제조하여 동일하게 실험 후 비교하였다.To 1 mL of sample, 1 mL of 0.2 M phosphate buffer (pH 6.6) and 1 mL of 1% potassium ferricyanide were added, mixed, and reacted at 50°C for 20 minutes. After adding and mixing 1 mL of 10% trichloroacetic acid (TCA), 2 mL of distilled water and 400 μL of 0.1% ferric chloride were mixed with 2 mL of the supernatant from the reaction solution. After reacting in the dark for 10 minutes, absorbance was measured at 700 nm using a spectrophotometer (Optizen 2120UV, Mecasys Co., Daejeon, Korea). L-ascorbic acid (DaeJung chemicals & Metals co., Ltd., Siheung, Korea) was prepared at a concentration of 10 ppm and compared after the same experiment.

즉석밥에 사용된 해조류의 환원력은 총폴리페놀 함량이 높은 톳에서 가장 높았으며 미역 및 모자반에서도 시험구 중 다소 높은 것으로 확인되었다. 총폴리페놀함량이 높을수록 환원력이 증가하는 것으로 알려지고 있는데, 해조즉석밥의 환원력은 표 15의 결과와 같이 총폴리페놀 함량이 높은 혼합곡물과 보리를 원료로 한 즉석밥에서 높게 나타났으며 에탄올추출물이 메탄올추출물보다 높게 나타났다.The reducing power of seaweeds used in instant rice was highest in shiitake which had a high total polyphenol content, and it was confirmed that seaweed and motherwort were also somewhat higher among the test groups. It is known that the reducing power increases as the total polyphenol content increases. As shown in Table 15, the reducing power of seaweed instant rice was high in mixed grains with high total polyphenol content and instant rice made from barley. The extract was found to be higher than that of the methanol extract.

[표 15] 해조류 즉석밥의 환원력[Table 15] Reducing Power of Seaweed Instant Rice

Figure 112019103013259-pat00015
Figure 112019103013259-pat00015

c. DPPH 라디칼 소거능c. DPPH radical scavenging ability

DPPH (2,2-diphenyl-1-picryl-hydrazyl, Sigma-Aldrich) 라디칼 소거 활성은 Lee (2019)의 방법을 응용하여 측정하였다. 1 mM DPPH 시약 제조 후 517 nm에서 흡광도가 1.0±0.05 사이가 되도록 에탄올과 혼합하면서 조절하였다. 조절한 DPPH 시약 140 μL, 시료 70 μL을 잘 혼합 후, 암소에서 30분간 반응시켰다. Microplate reader (EpochTM,BioTekInstruments,Inc.,Winooski,VT,USA)를 이용하여 517 nm에서 흡광도를 측정하였다. 대조구는 추출 용매를 사용하여 실험하였으며, DPPH 라디칼 소거 활성은 아래의 식으로 계산하여 백분율로 나타내었다. L-ascorbic acid (DaeJung chemicals & Metals co., Ltd., Siheung, Korea) 10 ppm 농도로 제조하여 동일하게 실험 후 비교하였다.DPPH (2,2-diphenyl-1-picryl-hydrazyl, Sigma-Aldrich) radical scavenging activity was measured by applying the method of Lee (2019). After preparing 1 mM DPPH reagent, it was adjusted while mixing with ethanol so that the absorbance at 517 nm was between 1.0±0.05. 140 µL of the adjusted DPPH reagent and 70 µL of the sample were mixed well, and then reacted in the dark for 30 minutes. Absorbance was measured at 517 nm using a microplate reader (Epoch™, BioTek Instruments, Inc., Winooski, VT, USA). The control was tested using the extraction solvent, and the DPPH radical scavenging activity was calculated by the following formula and expressed as a percentage. L-ascorbic acid (DaeJung chemicals & Metals co., Ltd., Siheung, Korea) was prepared at a concentration of 10 ppm and compared after the same experiment.

DPPH free radical scavenging (%) = 1-(As/Ac) ×100DPPH free radical scavenging (%) = 1-(A s /A c ) ×100

As : absorbance of control at 517nmA s : absorbance of control at 517nm

Ac : absorbance of control at 517nmA c : absorbance of control at 517nm

항산화활성을 평가하는데 중요 지표롤 사용되고 있는 DPPH 라디칼 소거활성은 미역과 톳에서 가장 높은 것으로 확인되었다. 미역과 톳에서 항산화활성이 높게 나타난 것은 총폴리페놀 함량 및 환원력과 밀접한 관계가 있는 것으로 판단된다. DPPH radical scavenging activity, which is used as an important index to evaluate antioxidant activity, was confirmed to be the highest in seaweed and shiitake. The high antioxidant activity of seaweed and seaweed is considered to be closely related to total polyphenol content and reducing power.

해조류 즉석밥에서는 표 16의 결과와 같이 보리를 원료로 한 샘플에서 약 31.1~34.7%로 가장 높은 소거활성을 보였으며 에탄올 추출물 보다는 메탄올 추출물에서 약간 높은 활성을 나타내었다. 또한 혼합곡류를 원료로 한 해조즉석밥에서도 시험구 중 DPPH 소거활성이 높은 것으로 나타났다. As shown in the results of Table 16, the seaweed instant rice showed the highest scavenging activity (about 31.1~34.7%) in the barley-based sample, and slightly higher activity in the methanol extract than in the ethanol extract. In addition, it was found that the DPPH scavenging activity was high in the test group in the instant rice of seaweed using mixed grains as a raw material.

[표 16] 해조류 즉석밥의 DPPH 라디컬 소거활성[Table 16] DPPH radical scavenging activity of seaweed instant rice

Figure 112019103013259-pat00016
Figure 112019103013259-pat00016

d. ABTS 라디칼 소거능d. ABTS radical scavenging ability

ABTS 라디칼 소거 활성은 Sung 등(2018)의 방법을 응용하여 측정하였다. 7 mM ABTS (Roche Diagnostics GMBH, Mannheim, Germany)와 2.45 mM potassium persulfate (Sigma-Aldrich)를 실온, 암소에서 16시간 이상 반응시켜 ABTS 양이온을 형성시켰다. 사용 직전에 흡광도가 0.8±0.02가 되도록 조정하였다. 시료를 20 μL에 ABTS 용액 180 μL를 혼합하여 실온에서 6분 동안 반응시켰다. Microplate reader (BioTek Instruments)를 이용하여 734 nm에서 흡광도를 측정하였다. 대조구는 추출용매를 사용하여 실험하였으며, ABTS 라디칼 소거 활성은 아래의 식으로 계산하여 백분율로 나타내었다. L-ascorbic acid (DaeJung chemicals & Metals co., Ltd., Siheung, Korea) 10 ppm 농도로 제조하여 동일하게 실험 후 비교하였다.ABTS radical scavenging activity was measured by applying the method of Sung et al. (2018). 7 mM ABTS (Roche Diagnostics GMBH, Mannheim, Germany) and 2.45 mM potassium persulfate (Sigma-Aldrich) were reacted for at least 16 hours at room temperature in the dark to form ABTS cations. Immediately before use, the absorbance was adjusted to 0.8±0.02. The sample was reacted for 6 minutes at room temperature by mixing 180 μL of ABTS solution in 20 μL. Absorbance was measured at 734 nm using a Microplate reader (BioTek Instruments). The control was tested using an extraction solvent, and the ABTS radical scavenging activity was calculated by the following formula and expressed as a percentage. L-ascorbic acid (DaeJung chemicals & Metals co., Ltd., Siheung, Korea) was prepared at a concentration of 10 ppm and compared after the same experiment.

ABTS radical scavenging (%) = 1-(As/Ac)100ABTS radical scavenging (%) = 1-(A s /A c )100

As : absorbance of control at 734nmA s : absorbance of control at 734nm

Ac : absorbance of control at 734nmA c : absorbance of control at 734nm

ABTS 라디칼 소거활성은 톳에서 가장 높게 평가되었으며 미역에서도 높은 억제활성을 보였고, 에탄올추추물이 메탄올 추출물보다 높게 억제활성으로 보여 DPPH 라디칼 소거활성과 유사한 경향을 확인하였다. The ABTS radical scavenging activity was evaluated to be the highest in Shiitake, and also showed a high inhibitory activity in seaweed, and the ethanol extract showed a higher inhibitory activity than the methanol extract, confirming a similar tendency to the DPPH radical scavenging activity.

해조류 즉석밥의 ABTS 라디칼소거활성은 표 17의 결과와 같이 메탄올추출물 시험구 중 보리 즉석밥에서 가장 높게 나타났으며 그 다음은 혼합곡물을 사용한 즉석밥에서 높게 나타나 DPPH라디칼 소거활성과 유사한 경향을 나타났다. As shown in Table 17, the ABTS radical scavenging activity of seaweed instant rice was highest in barley instant rice among the methanol extract test groups, followed by the highest in instant rice using mixed grains, showing a similar tendency to DPPH radical scavenging activity. .

[표 17] 해조류 즉석밥의 ABTS 라디컬 소거활성[Table 17] ABTS radical scavenging activity of seaweed instant rice

Figure 112019103013259-pat00017
Figure 112019103013259-pat00017

총폴리페놀 함량이 높으면서 항산화활성이 높은 해조류는 톳 > 미역 > 모자반 순이었으며 이들을 해조류 즉석밥 제조에 사용할 경우 항산화 활성이 높은 즉석밥이 될 것으로 기대된다. 또한, 항산화활성 측면에서 해조류 즉석밥의 곡류는 백미만 사용하는 것보다 보리나 혼합곡을 함께 사용할 필요가 있다. Seaweeds with high total polyphenol content and high antioxidant activity were in the order of shiitake > seaweed > mackerel. In addition, in terms of antioxidant activity, it is necessary to use barley or mixed grains together, rather than using only white rice, for grains of seaweed instant rice.

이상에서 바람직한 실시예를 통하여 본 발명을 예시적으로 설명하였으나, 본 발명은 이와 같은 특정 실시예에만 한정되는 것은 아니며 본 발명에서 제시한 기술적 사상, 구체적으로는 특허청구범위에 기재된 범주 내에서 다양한 형태로 수정, 변경, 또는 개선될 수 있을 것이다.Although the present invention has been exemplarily described through preferred embodiments above, the present invention is not limited to such specific embodiments, and various forms within the scope of the technical idea presented in the present invention, specifically, the claims may be modified, changed, or improved.

Claims (4)

백미, 보리, 잡곡 중에서 선택되는 곡류 45 ~ 50 중량%와, 해조류 8 ~ 12중량%와, 정제수 40 ~ 45 중량%를 포함하여 구성되는 즉석밥 재료를 준비하고,
각 재료를 세척하여 용기에 투입하여 1차 포장하고,
포장된 즉석밥 재료를 레토르트 투입하여 증숙 및 살균하고,
증숙된 즉석밥 재료를 냉각한 후 상온 건조하여 2차 포장하는 단계를 포함하며,
상기 잡곡은 현미, 찹쌀, 찰흑미, 늘보리, 현미찹쌀, 발아현미, 납작보리, 검정콩, 붉은팥, 강남콩, 찰수수, 찰기장, 홍미, 흑미, 검정콩, 녹미, 보리 중에서 선택되는 두 종류 이상을 포함하고,
상기 해조류는 톳과 모자반 및 미역을 포함하며, 톳과 미역은 동일한 비율로 포함되고 모자반은 톳보다 함량이 큰 것을 특징으로 하며,
상기 해조류는 용기 투입 전 전처리 과정으로 80 ~ 95℃의 온도에서 2 ~ 10분간 블랜칭을 실시하는 것을 특징으로 하는
해조류 즉석밥 제조방법.
Prepare an instant rice material comprising 45 to 50% by weight of grains selected from white rice, barley, and mixed grains, 8 to 12% by weight of seaweed, and 40 to 45% by weight of purified water,
Each material is washed and put into a container for primary packaging,
Steaming and sterilizing the packaged instant rice ingredients by adding retort,
It comprises the step of cooling the steamed instant rice material and then drying it at room temperature for secondary packaging,
The mixed grains include two or more types selected from brown rice, glutinous rice, sticky black rice, sloth barley, brown rice glutinous rice, germinated brown rice, flat barley, black beans, red beans, Gangnam beans, sorghum, sticky rice, red rice, black rice, black beans, green rice, and barley ,
The seaweeds include shiitake, mother-of-pearl, and seaweed, wherein the seaweed and seaweed are included in the same ratio, and the mother-and-a-half is characterized in that the content is greater than that of seaweed,
The seaweed is a pretreatment process before input into a container, characterized in that blanching is performed for 2 to 10 minutes at a temperature of 80 to 95 ° C.
How to make instant seaweed rice.
삭제delete 삭제delete 제1항에 있어서,
상기 곡류는 백미에 보리 또는 잡곡이 혼합된 것을 사용하는 것을 특징으로 하는 해조류 즉석밥 제조방법.
According to claim 1,
The method for preparing instant seaweed rice, characterized in that the grain uses a mixture of white rice and barley or mixed grains.
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