KR102296932B1 - Oil composition improved rancidity inhibition and tortilla using the same - Google Patents

Oil composition improved rancidity inhibition and tortilla using the same Download PDF

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KR102296932B1
KR102296932B1 KR1020200159139A KR20200159139A KR102296932B1 KR 102296932 B1 KR102296932 B1 KR 102296932B1 KR 1020200159139 A KR1020200159139 A KR 1020200159139A KR 20200159139 A KR20200159139 A KR 20200159139A KR 102296932 B1 KR102296932 B1 KR 102296932B1
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oil
rancidity
composition
weight
green tea
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KR1020200159139A
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Korean (ko)
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김병욱
김대중
임재천
이혜림
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(주)자연인 에프앤씨
강원대학교산학협력단
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings, cooking oils
    • A23D9/06Preservation of finished products
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D13/00Finished or partly finished bakery products
    • A21D13/40Products characterised by the type, form or use
    • A21D13/42Tortillas
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D13/00Finished or partly finished bakery products
    • A21D13/60Deep-fried products, e.g. doughnuts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings, cooking oils
    • A23D9/007Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts

Abstract

The present invention relates to an oil-fat composition having improved rancidity inhibition ability, and provides a composition having an excellent effect of inhibiting or delaying rancidity of oils and fats, including a natural antioxidant. In addition, the present invention provides a tortilla capable of maintaining quality for a long time according to an effect of inhibiting or delaying oil-fat rancidity.

Description

산패 억제능이 향상된 유지 조성물 및 이를 이용한 토르티야 {Oil composition improved rancidity inhibition and tortilla using the same}Oil composition with improved rancidity inhibition and tortilla using the same {Oil composition improved rancidity inhibition and tortilla using the same}

본 발명은 천연 항산화제를 이용하여 산패 억제능이 향상된 유지 조성물과 이를 이용한 산패 억제능이 향상된 토르티야에 관한 것이다.The present invention relates to an oil composition with improved rancidity inhibiting ability using natural antioxidants and tortillas with improved rancidity inhibiting ability using the same.

유지는 대기 중의 산소와 접촉하면 자동 산화라는 일련의 라디칼(Radical) 연쇄 반응이 진행되어 과산화물을 생성한다. 생성된 과산화물은 비교적 불안정한 물질로, 중합 또는 분해되어 변패취의 주요 성분으로 작용하는 카보닐 등 각종 화합물을 생성하여 유지의 풍미를 변화시키고 품질 저하를 초래한다.When oils and fats come into contact with oxygen in the atmosphere, a series of radical chain reactions called auto-oxidation proceed to generate peroxides. The produced peroxide is a relatively unstable substance, and it polymerizes or decomposes to produce various compounds such as carbonyl, which act as a major component of the odor, thereby changing the flavor of the oil and causing deterioration of the quality.

이렇게 생성된 과산화지질은 성인병의 원인이 되거나 노화 등 유해성이 있어 문제가 될 수 있다. 따라서 유지의 산화를 방지하기 위한 부틸히드록시아니솔(butylhydroxy anisole, BHA), 디부틸히드록시톨루엔(dibutyl hydroxy toluene, BHT) 등 합성산화방지제가 이용되지만, 합성산화방지제는 안정성 문제로 사용량이 제한되어 있다. 이에 최근에는 천연산화방지제를 이용하고자 하는 시도가 이루어지고 있다.The lipid peroxide produced in this way can cause adult diseases or have harmful effects such as aging, which can be a problem. Therefore, synthetic antioxidants such as butylhydroxy anisole (BHA) and dibutyl hydroxy toluene (BHT) are used to prevent oxidation of oils and fats, but the use of synthetic antioxidants is limited due to stability problems. has been In recent years, attempts have been made to use natural antioxidants.

대한민국 공개특허 제10-2020-0001033호(2020.01.06.)에는 우수한 산패 억제능력을 갖는 유지 조성물이 기재되어 있다.Korean Patent Laid-Open No. 10-2020-0001033 (2020.01.06.) discloses an oil-fat composition having excellent rancidity inhibition ability. 대한민국 공개특허 제1-2018-0004054호(2018.01.10.)에는 유지의 항산화용 조성물, 이의 제조방법, 이를 함유하는 식용유 및 상기 식용유의 제조방법이 기재되어 있다.Korean Patent Laid-Open No. 1-2018-0004054 (2018.01.10.) describes a composition for antioxidants of fats and oils, a method for preparing the same, an edible oil containing the same, and a method for preparing the edible oil.

본 발명은 천연산화방지제를 이용하여 유지의 산패를 억제 또는 지연하고자 하였다.The present invention was to suppress or delay rancidity of fats and oils by using a natural antioxidant.

본 발명은 유지의 산패가 억제 또는 지연됨에 따라 유통 또는 저장 후에도 관능이 우수한 식품 조성물을 제공하고자 한다.An object of the present invention is to provide a food composition excellent in sensory even after distribution or storage as rancidity of oils and fats is suppressed or delayed.

본 발명은 녹차추출물 및 로즈마리추출물을 포함하는 유지 산패 억제용 조성물을 제공한다.The present invention provides a composition for inhibiting oil rancidity comprising a green tea extract and a rosemary extract.

본 발명에 있어서, 상기 녹차추출물 또는 로즈마리추출물은, 바람직하게는 추출용매로 물 또는 에탄올을 이용한 것이 좋다.In the present invention, the green tea extract or rosemary extract is preferably water or ethanol as an extraction solvent.

또한, 본 발명은 유지 전체 중량에 대하여, 녹차추출물 0.109~0.111 중량%, 로즈마리추출물 0.088~0.090 중량%이 첨가된 유지 조성물을 제공한다.In addition, the present invention provides an oil-fat composition in which 0.109 to 0.111 wt% of green tea extract and 0.088 to 0.090 wt% of rosemary extract are added, based on the total weight of the oil.

본 발명에 있어서, 상기 유지는, 바람직하게는 식물성 유지인 것이 좋다.In the present invention, the oil is preferably vegetable oil.

본 발명에 있어서, 상기 녹차추출물 또는 로즈마리추출물은, 바람직하게는 추출용매로 물 또는 에탄올을 이용한 것이 좋다.In the present invention, the green tea extract or rosemary extract is preferably water or ethanol as an extraction solvent.

또한, 본 발명은 유지 전체에 대하여, 녹차추출물 0.109~0.111 중량%, 로즈마리추출물 0.088~0.090 중량%를 포함하는 유지 조성물을 제조하는 단계 (a); 및 토르티야(tortilla) 반죽을 상기 유지 조성물에 넣고 유탕처리하는 단계 (b);를 포함하는 것을 특징으로 하는 토르티야 제조방법을 제공한다.In addition, the present invention, with respect to the whole oil and fat, 0.109 ~ 0.111% by weight of green tea extract, preparing an oil and fat composition comprising 0.088 ~ 0.090% by weight of rosemary extract (a); and (b) putting the tortilla dough into the oil-fat composition and heat-treating it.

본 발명에 있어서, 상기 유탕 처리는, 바람직하게는 150~200℃에서 5~10분간 유탕처리하는 것이 좋다.In the present invention, the oil bath treatment is preferably performed at 150 to 200 ° C. for 5 to 10 minutes.

본 발명은 천연산화방지제를 특정 배합비로 포함하여 유지의 산패 억제 또는 산패 지연 효과가 우수한 유지 산패 억제용 조성물을 제공한다.The present invention provides a composition for inhibiting rancidity of oils and fats excellent in inhibiting rancidity or delaying rancidity of oils and fats by including a natural antioxidant in a specific mixing ratio.

본 발명은 유지 산패 억제 또는 산패 지연 효과에 따라 장기간 품질 유지가 가능한 식품 조성물을 제공한다.The present invention provides a food composition capable of maintaining quality for a long time according to the effect of inhibiting rancidity or delaying rancidity.

도 1은 천연산화방지제의 AOM 값을 나타낸 그래프이다.
도 2에 RSM 분석법에 의한 천연산화방지제 3종에 대한 반응궤적도이다.
도 4은 RSM 분석법에 의한 천연산화방지제 3종의 혼합물 등고선도이다.
도 4는 RSM 분석법에 의한 천연산화방지제 3종의 혼합물 표면도이다.
도 5는 유지 산패 억제용 조성물을 포함하는 유지 조성물의 AOM 값을 나타낸 그래프이다.
도 6은 유지 산패 억제용 조성물을 포함하는 유지 조성물의 지질과산화값을 나타낸 그래프이다.
도 7은 유지 산패 억제용 조성물을 포함하는 유지 조성물의 과산화물가를 나타낸 그래프이다.
도 8은 유지 산패 억제용 조성물을 포함하는 유지 조성물의 산가를 나타낸 그래프이다.
도 9는 유지 산패 억제용 조성물을 포함하는 유지 조성물을 이용하여 유탕 처리 한 토르티야의 AOM 값을 나타낸 그래프이다.
도 10은 속재료를 포함하는 토르티야 조리 후의 특성별 기호도 검사 결과를 나타낸 그래프이다.
도 11은 속재료를 포함하는 토르티야 조리 후의 특성별 정도 검사 결과를 나타낸 그래프이다.
1 is a graph showing the AOM value of natural antioxidants.
2 is a reaction trajectory diagram for three types of natural antioxidants by RSM analysis.
4 is a contour diagram of a mixture of three types of natural antioxidants by RSM analysis.
4 is a surface view of a mixture of three types of natural antioxidants by RSM analysis.
5 is a graph showing the AOM value of the oil composition comprising the composition for inhibiting oil rancidity.
6 is a graph showing the lipid peroxidation value of the oil composition comprising the composition for inhibiting oil rancidity.
7 is a graph showing the peroxide value of a fat or oil composition comprising a composition for inhibiting rancidity of fats and oils.
8 is a graph showing the acid value of a fat or oil composition comprising a composition for inhibiting rancidity of fats and oils.
9 is a graph showing the AOM value of tortillas oil-treated using an oil-fat composition comprising a composition for inhibiting oil rancidity.
10 is a graph showing the test results for each characteristic after cooking tortillas including ingredients.
11 is a graph showing the test results for each characteristic after cooking tortillas including ingredients.

본 발명은 녹차추출물 및 로즈마리추출물을 포함하는 유지 산패 억제용 조성물을 제공한다. 또한, 본 발명은 유지 산패 억제용 조성물을 포함하는 유지 조성물을 제공한다. 유지는 산화에 따라 풍미가 변화되고 품질 저하가 발생되는데, 본 발명은 천연산화방지제를 이용하여 유지의 산패를 억제 또는 지연시키고자 하였다.The present invention provides a composition for inhibiting oil rancidity comprising a green tea extract and a rosemary extract. In addition, the present invention provides an oil-fat composition comprising a composition for inhibiting oil-and-oil rancidity. Fats and oils have a change in flavor and deterioration in quality according to oxidation, and the present invention was intended to inhibit or delay rancidity of oils and fats by using a natural antioxidant.

천연산화방지제로 알려진 토코페롤(비타민 E), 로즈마리추출물, 녹차추출물, 허브티추출물(로즈마리추출물 50 중량% 및 녹차추출물 50 중량%로 구성, 이하 허브티 추출물), 미강추출물을 각각 단독으로 이용하여 유지의 산화안정도를 비교한 결과, 유지의 산패 억제 또는 지연 효과가 우수한 물질은 녹차추출물, 로즈마리추출물, 허브티추출물로 나타났다. 본 발명은 이들을 혼합하여 최대 효율의 산화안정 효과를 도출하고자, 반응표면분석법을 통해 최적 혼합비율을 도출하였다.Tocopherol (vitamin E), which is known as a natural antioxidant, rosemary extract, green tea extract, herbal tea extract (composed of 50% by weight of rosemary extract and 50% by weight of green tea extract, hereinafter herbal tea extract), rice bran extract As a result of comparing the oxidation stability of fats and oils, green tea extract, rosemary extract, and herbal tea extract were found to be excellent substances in inhibiting or delaying rancidity of oils and fats. In the present invention, to derive the oxidation stabilization effect of maximum efficiency by mixing them, the optimum mixing ratio was derived through the reaction surface analysis method.

반응표면분석법을 통해 도출된 유지 산패 억제용 조성물의 최적 혼합비율은 녹차추출물 0.110 중량% 및 로즈마리추출물 0.0890 중량%로 나타났다. 즉, 본 발명의 유지 산패 억제용 조성물은, 바람직하게는 녹차추출물 0.109~0.111 중량부와 로즈마리추출물 0.088~0.090 중량부의 비율로 혼합하여 조성되는 것이 좋다. 즉, 허브티추출물의 유지 산패 억제 또는 지연 효과가 우수하지만, 녹차추출물과 로즈마리추출물을 동시에 이용함에 따라 유지 산패 억제 효과가 감소할 수 있다.The optimal mixing ratio of the composition for inhibiting oil rancidity derived through the response surface analysis was found to be 0.110 wt% of green tea extract and 0.0890 wt% of rosemary extract. That is, the composition for inhibiting oil rancidity of the present invention is preferably mixed in a ratio of 0.109 to 0.111 parts by weight of green tea extract and 0.088 to 0.090 parts by weight of rosemary extract. That is, although the herbal tea extract has an excellent effect of inhibiting or delaying oil rancidity, as the green tea extract and rosemary extract are used at the same time, the oil and fat rancidity inhibitory effect may be reduced.

한편, 천연산화방지제를 혼합한 유지 산패 억제용 조성물은 고가의 비용인 점 및 단독으로 사용했을 때의 산패 억제능을 고려하여, 유지 전체에 대하여 유지 전체에 대하여 0 중량% 초과 0.3 중량% 이하로 첨가되는 것이 좋으며, 바람직하게는 유지 전체에 대하여 0 중량% 초과 0.2 중량% 이하로 첨가되는 것이 좋다.On the other hand, the composition for inhibiting rancidity of oils and fats mixed with natural antioxidants is added in an amount of more than 0% by weight and not more than 0.3% by weight with respect to the whole fats and oils, in consideration of the high cost and the ability to inhibit rancidity when used alone Preferably, it is added in an amount of more than 0% by weight and not more than 0.2% by weight based on the whole fat.

이에, 본 발명은 유지 전체 중량에 대하여, 녹차추출물 0.109~0.111 중량%, 로즈마리추출물 0.088~0.090 중량%를 포함하는 유지 조성물을 제공한다. Accordingly, the present invention provides an oil and fat composition comprising 0.109 to 0.111 wt% of green tea extract, and 0.088 to 0.090 wt% of rosemary extract, based on the total weight of fats and oils.

본 발명에 있어서, 상기 유지는 바람직하게는 식물성 유지인 것이 좋으나, 동물성 유지에도 사용 가능하다.In the present invention, the fats and oils are preferably vegetable oils and fats, but can also be used for animal fats and oils.

본 발명의 유지 산패 억제용 조성물을 포함하는 유지의 산패 정도를 측정하기 위해 토르티야(tortilla)를 반복 유탕 처리 함에 따른 산화안정도, 지질과산화도, 과산화물가, 산가를 분석하였다. 본 발명의 유지 산패 억제용 조성물을 포함하는 유지 조성물은 대조군(유지 산패 억제용 조성물 미포함)에 비해 산패 억제 또는 지연 효과가 우수한 점을 확인하였다.In order to measure the degree of rancidity of fats and oils containing the composition for inhibiting fats and oils of the present invention, oxidation stability, lipid peroxidation, peroxide value, and acid value were analyzed by repeatedly scalding tortillas. It was confirmed that the oil-fat composition comprising the composition for inhibiting oil-fat rancidity of the present invention was superior to the control (not including the composition for inhibiting oil-oil rancidity) in inhibiting or delaying rancidity.

또한, 상기 반복 유탕 처리 한 토르티야도 본 발명의 유지 산패 억제용 조성물을 포함하는 유지 조성물을 이용한 경우가 대조군(유지 산패 억제용 조성물 미포함)을 이용한 경우에 비해 산화안정도가 높은 것으로 나타났다.In addition, the case of using the oil-fat composition containing the composition for inhibiting oil rancidity of the present invention also showed that the tortilla subjected to the repeated oil bath treatment had higher oxidative stability compared to the case of using the control (not including the composition for inhibiting oil and fat rancidity).

즉, 본 발명의 유지 산패 억제용 조성물을 포함하는 유지 조성물을 이용하여 토르티야 반죽을 유탕처리 할 경우 토르티야의 품질이 장기간 유지될 수 있다.That is, when the tortilla dough is oil-treated using the oil-fat composition including the composition for inhibiting oil-and-oil rancidity of the present invention, the quality of the tortilla can be maintained for a long time.

또한, 본 발명은 유지 전체에 대하여, 녹차추출물 0.109~0.111 중량%, 로즈마리추출물 0.088~0.090 중량%를 포함하는 유지 조성물을 제조하는 단계 (a); 및 토르티야(tortilla) 반죽을 상기 유지 조성물에 넣고 유탕처리하는 단계 (b);를 포함하는 것을 특징으로 하는 토르티야 제조방법을 제공한다.In addition, the present invention, with respect to the whole oil and fat, 0.109 ~ 0.111% by weight of green tea extract, preparing an oil and fat composition comprising 0.088 ~ 0.090% by weight of rosemary extract (a); and (b) putting the tortilla dough into the oil-fat composition and heat-treating it.

한편, 본 발명에 있어서, 상기 유탕 처리는, 150~200℃에서 5~10분간 유탕처리하는 것이 좋다.Meanwhile, in the present invention, the oil bath treatment is preferably performed at 150 to 200° C. for 5 to 10 minutes.

본 발명의 토르티야 반죽은 바람직하게는, 토르티야 반죽 전체 중량에 대하여, 중력분 40~50 중량%, 설탕 1~10 중량%, 베이킹파우더 0.001~2 중량% 및 정제수 40~50 중량%를 혼합하여 반죽하는 것이 좋으며, 속재료를 포함하는 토르티야를 제조하기 위해서는 이를 반조리 할 수 있다. 즉, 160~200℃에서 2~6 분간 조리하여 반조리된 토르티야를 이용할 수 있다.The tortilla dough of the present invention is preferably, with respect to the total weight of the tortilla dough, 40 to 50% by weight of gravity flour, 1 to 10% by weight of sugar, 0.001 to 2% by weight of baking powder, and 40 to 50% by weight of purified water to knead. It is good, and it can be semi-cooked to make tortillas with ingredients. That is, semi-cooked tortillas can be used by cooking at 160-200° C. for 2-6 minutes.

토르티야 속재료는 속재료 전체 중량에 대하여, 붉은대게살 10~15 중량%, 돼지고기 16~22 중량%, 소스 20~30 중량%, 양파 10~15 중량%, 홍새우살 10~15 중량%, 옥수수알갱이 2~8 중량%, 모짜렐라치즈 10~5 중량%로 구성하는 것이 좋다. 붉은대게살, 돼지고기, 양파, 홍새우살, 옥수수알갱이는 반조리된 것을 사용하는 것이 좋다. 상기 소스는 바람직하게는 소스 전체 중량에 대하여 토마토 스파게티 소스 75~81 중량%, 토마토 케첩 16~23 중량%, 설탕 0.001~4 중량%로 구성하는 것이 좋다. 토마토 스파게티 소스와 케첩은 시중에서 판매중인 것을 사용할 수 있으며, 당 업계에서 통상적으로 제조하는 토마토 스파게티 소스와 토마토 케첩을 이용할 수 있다. 또한 상기 옥수수알갱이는 당 업계에서 통상적으로 사용하는 스위트콘을 사용하는 것이 바람직하다.The ingredients for tortilla are 10-15 wt% of red snow crab meat, 16-22 wt% of pork, 20-30 wt% of sauce, 10-15 wt% of onion, 10-15 wt% of red shrimp, corn based on the total weight of the ingredients. It is recommended to consist of 2 to 8% by weight of grains and 10 to 5% by weight of mozzarella cheese. It is better to use semi-cooked pieces of red snow crab, pork, onion, red shrimp, and corn kernels. The sauce is preferably composed of 75 to 81% by weight of tomato spaghetti sauce, 16 to 23% by weight of tomato ketchup, and 0.001 to 4% by weight of sugar based on the total weight of the sauce. Tomato spaghetti sauce and ketchup may be commercially available, and tomato spaghetti sauce and tomato ketchup commonly prepared in the art may be used. In addition, it is preferable to use sweet corn commonly used in the art for the corn kernels.

본 발명에서 제조한 속재료를 포함하는 토르티야는 유지 산패 억제용 조성물을 포함하는 유지 조성물을 이용하여 유탕처리 되었으며, 관능평가 결과, 토르티야의 바삭한 식감이 우수한 것으로 나타났다.The tortilla containing the filling material prepared in the present invention was oil-heated using an oil-fat composition containing a composition for inhibiting oil rancidity, and as a result of sensory evaluation, it was found that the tortilla had excellent crispy texture.

한편, 본 발명의 토르티야는 속초시의 특화자원인 붉은 대게를 포함한다. 붉은 대게(홍게)는 껍질이 딱딱하고 속이 꽉 차지 않으며, 몸통은 진홍색으로 등쪽과 배쪽이 모두 붉은 특징이 있다. 붉은 대게는 가식부 100g 당 열량이 46kcal로, 다른 게에 비해 열량이 낮고, 단백질은 10.3g으로 대게(8.0g)에 비해 높은 반면, 지방 함량은 0.3g으로 꽃게(0.9g)와 대게(2.6g)에 비해 상대적으로 저지방 식품이다. 붉은 대게는 칼슘 211mg, 철분 1.5mg, 아연 등의 필수 미네랄과 메티오닌, 라이신 등 함황아미노산, 글리신, 알라닌, 트레오닌, 세린, 글루탐산 등의 정미성 아미노산 등이 함유되어 대게의 칼슘 78mg, 철분1.1 mg 등에 비해 있는 영양학적으로 우수한 것으로 알려져 있다.On the other hand, the tortilla of the present invention includes red snow crab, a specialized resource of Sokcho. The red snow crab (red crab) has a hard shell and not full inside. Red snow crab has 46 kcal of calories per 100 g of edible part, lower than other crabs, and has 10.3 g of protein, which is higher than snow crab (8.0 g), but has 0.3 g of fat content, such as blue crab (0.9 g) and snow crab (2.6 g). ) is a relatively low-fat food. Red snow crab contains essential minerals such as calcium 211mg, iron 1.5mg, zinc, sulfur-containing amino acids such as methionine and lysine, and refined amino acids such as glycine, alanine, threonine, serine, and glutamic acid. It is known to be nutritionally superior compared to its predecessors.

한편, 본 발명에 있어서, 상기 녹차추출물 또는 로즈마리추출물은 당업계에 공지된 통상의 방법에 따라, 즉 통상적인 온도와 압력의 조건 하에서 통상적인 용매를 사용하여 제조될 수 있으며, 일 예로 물, 탄소수 1-4의 무수 또는 함수 저급 알코올, 프로필렌글리콜, 부틸렌글리콜, 글리세린, 아세톤, 에틸 아세테이트, 클로로포름, 부틸 아세테이트, 디에틸에테르, 디클로로메탄, 헥산 및 이들의 혼합물로 구성된 군으로부터 선택되는 추출용매를 이용한 용매 추출법, 초임계 추출법 및 초음파 추출법 중 선택되는 어느 하나의 추출법을 통해 수득될 수 있다.On the other hand, in the present invention, the green tea extract or rosemary extract can be prepared according to a conventional method known in the art, that is, using a conventional solvent under conditions of conventional temperature and pressure, for example, water, carbon number An extraction solvent selected from the group consisting of anhydrous or aqueous lower alcohols of 1-4, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane, and mixtures thereof It can be obtained through any one extraction method selected from the solvent extraction method, supercritical extraction method, and ultrasonic extraction method used.

이하, 본 발명에 대해 하기 실시예 및 실험예에서 더욱 상세히 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예 및 실험예에만 한정되는 것은 아니고, 이와 등가의 기술적 사상의 변형까지를 모두 포함한다.Hereinafter, the present invention will be described in more detail in the following Examples and Experimental Examples. However, the scope of the present invention is not limited only to the following examples and experimental examples, and includes all modifications of the technical idea equivalent thereto.

[실험예 1 : 천연산화방지제 종류별 산화안정도 측정][Experimental Example 1: Measurement of Oxidation Stability by Type of Natural Antioxidant]

본 실험에서는 천연산화방지제로 알려진 토코페롤(분말비타민E 50 중량% 및 DL-알파토코페릴아세테이트 50 중량%), 로즈마리추출물, 녹차추출물, 허브티추출물(로즈마리추출물 50 중량% 및 녹차추출물 50 중량%로 구성, 이하 허브티 추출물), 미강추출물을 각각 단독으로 이용하여 유지의 산화안정도를 측정하였다.In this experiment, tocopherol (50% by weight of powdered vitamin E and 50% by weight of DL-alpha tocopheryl acetate), rosemary extract, green tea extract, herbal tea extract (50% by weight of rosemary extract and 50% by weight of green tea extract), known as a natural antioxidant, was used in this experiment. Composition, hereinafter herbal tea extract) and rice bran extract were used individually to measure the oxidation stability of oils and fats.

로즈마리추출물은 로즈마리 건조중량 10g에 70 % (v/v)에탄올을 가하고 80℃에서 2시간 추출한 뒤, 농축 및 동결건조하여 고형분의 로즈마리추출물을 제조하였다. 녹차추출물, 허브티추출물, 미강추출물은 로즈마리추출물과 동일하게 제조하였다.For the rosemary extract, 70% (v/v) ethanol was added to 10 g of dry weight of rosemary, extracted at 80° C. for 2 hours, and concentrated and freeze-dried to prepare a solid rosemary extract. Green tea extract, herbal tea extract, and rice bran extract were prepared in the same way as rosemary extract.

각 천연산화방지제를 대두유의 전체 중량에 대하여 0~0.3 중량% 첨가하여 유지의 산화안정도를 측정(AOM test)하였다. 산화안정도 측정 시험은 고온에서 공기를 대량 공금하여 가속 산화를 유도하는 극한 조건에서 유지의 산화 안정성을 측정하는 방법이다. 이를 통해 유지에 첨가된 산화방지제의 효능을 비교할 수 있다. 산화안정도는 Oxidation tester(873 Biodiesel Rancimat, Metrohm 社)를 이용하여 측정하였다. 산화안정도는 100℃에서 유지를 가열하며, 계속해서 공기방울을 주입하는 방식으로 측정하였다. 실험은 Peroxide Values (PV)가 20에 도달하기까지의 시간을 관찰하였다. PV는 유지 1kg 당 과산화물의 mEq로 표현된다.Oxidation stability of fats and oils was measured (AOM test) by adding 0 to 0.3% by weight of each natural antioxidant based on the total weight of soybean oil. Oxidation stability measurement test is a method of measuring the oxidation stability of fats and oils under extreme conditions inducing accelerated oxidation by supplying a large amount of air at a high temperature. Through this, the efficacy of antioxidants added to fats and oils can be compared. Oxidation stability was measured using an oxidation tester (873 Biodiesel Rancimat, Metrohm). Oxidation stability was measured by heating fats and oils at 100°C and continuously injecting air bubbles. The experiment observed the time until the Peroxide Values (PV) reached 20. PV is expressed as mEq of peroxide per kg of fat.

도 1 및 표 1에 산화안정도 측정 결과를 나타내었다. 수치는 유지가 산화되는데 걸리는 시간을 나타낸다.1 and Table 1 show the measurement results of oxidation stability. The numerical value indicates the time it takes for fats and oils to be oxidized.

  토코페롤
(Vitamin E)
tocopherol
(Vitamin E)
녹차추출물green tea extract 로즈마리추출물rosemary extract 허브티추출물herbal tea extract 미강추출물Rice bran extract
ControlControl 13.59 h13.59 h 13.10 h13.10 h 13.32 h13.32 h 13.32 h13.32 h 12.86 h12.86 h 0.10%0.10% 13.58 h13.58 h 18.33 h18.33 h 15.06 h15.06 h 19.59 h19.59 h 12.60 h12.60 h 0.20%0.20% 13.68 h13.68 h 24.61 h24.61 h 18.84 h18.84 h 24.86 h24.86 h 12.39 h12.39 h 0.30%0.30% 13.42 h13.42 h 29.39 h29.39 h 21.84 h21.84 h 28.61 h28.61 h 12.54 h12.54 h

도 1 및 표 1에서 보듯이, 산화안정 효과가 우수한 소재는 녹차추출물, 로즈마리추출물, 허브티추출물로 나타났다. As shown in FIG. 1 and Table 1, the materials with excellent oxidation stabilization effect were green tea extract, rosemary extract, and herbal tea extract.

[실험예 2 : 유지 산패 억제용 혼합물의 최적 혼합비율 도출][Experimental Example 2: Derivation of optimal mixing ratio of mixture for inhibiting oil rancidity]

본 실험에서는 유지의 산패를 최대한 억제 또는 지연시키고자, 상기 실험예 1에서 산화안정 효과가 우수한 소재인 녹차추출물, 로즈마리추출물, 허브티추출물을 혼합하여 최대 효율의 산화안정 효과를 도출하고자 하였다.In this experiment, in order to suppress or delay the rancidity of oils and fats as much as possible, in Experimental Example 1, green tea extract, rosemary extract, and herbal tea extract, which are materials with excellent oxidation stabilization effect, were mixed to derive the maximum efficiency of oxidation stabilization effect.

각 소재에 대하여 RSM 분석법을 통해 최적 혼합비율을 설정하였으며, 실험의 설계방식은 반응표면분석법의 심플렉스 중심점을 이용하였다. 천연산화방지제가 고가인 점 및 상기 실험예 1에 따른 단독 실험 결과를 고려하여, 천연산화방지제의 첨가 함량은 유지 전체에 대하여 0 중량% 초과 0.2 중량% 이하로 설정하였다. 변수는 천연항산화제 3종으로 설정하고, 반응값은 유지의 AOM 값으로 설정하였다. 반복실험은 실험오차의 추정, 요인효과의 명확한 추정, 처리 조합 변동의 분석을 위해 실시하였고, 반복실험은 정점과 중앙점만 2회 반복하였다.For each material, the optimal mixing ratio was set through RSM analysis, and the simplex central point of the response surface analysis method was used for the design method of the experiment. In consideration of the high cost of natural antioxidants and the results of a single test according to Experimental Example 1, the added content of natural antioxidants was set to more than 0% by weight and not more than 0.2% by weight based on the whole fat. Variables were set as 3 types of natural antioxidants, and response values were set as AOM values of fats and oils. The repeated experiment was conducted to estimate the experimental error, to estimate the factor effect clearly, and to analyze the treatment combination variation.

표 2는 RSM 분석법 결과, 표 3은 AOM 값(성분비율)에 대한 추정된 회귀계수, 표 4는 AOM 값에 대한 분산분석(성분비율), 표 5는 AOM 값(성분 양)에 대한 추정된 회귀계수이다.Table 2 is the results of the RSM method, Table 3 is the estimated regression coefficient for the AOM value (component ratio), Table 4 is the ANOVA for the AOM value (component ratio), and Table 5 is the estimated regression coefficient for the AOM value (component ratio). is the regression coefficient.

표준
순서
Standard
order

순서
run
order

유형
dot
type
블럭block 녹차추출물green tea extract 로즈마리
추출물
Rosemary
extract
허브티추출물herbal tea extract AOM 값AOM value
77 1One 00 1One 0.0666670.066667 0.0666670.066667 0.0666670.066667 25.46h25.46h 1111 22 1One 1One 0.20.2 00 00 24.61h24.61h 99 33 -1-One 1One 0.0333330.033333 0.1333330.133333 0.0333330.033333 24.31h24.31h 1010 44 -1-One 1One 0.0333330.033333 0.0333330.033333 0.1333330.133333 24.61h24.61h 1414 55 00 1One 0.0666670.066667 0.0666670.066667 0.0666670.066667 25.10h25.10h 22 66 1One 1One 00 0.20.2 00 23.59h23.59h 1One 77 1One 1One 0.20.2 00 00 24.45h24.45h 1212 88 1One 1One 00 0.20.2 00 23.86h23.86h 1313 99 1One 1One 00 00 0.20.2 24.86h24.86h 55 1010 22 1One 0.10.1 00 0.10.1 24.02h24.02h 44 1111 22 1One 0.10.1 0.10.1 00 26.03h26.03h 33 1212 1One 1One 00 00 0.20.2 24.78h24.78h 66 1313 22 1One 00 0.10.1 0.10.1 24.26h24.26h 88 1414 -1-One 1One 0.1333330.133333 0.0333330.033333 0.0333330.033333 24.66h24.66h

port 계수Coefficient SE계수SE coefficient TT PP VIFVIF 녹차추출물green tea extract 24.48024.480 0.20870.2087 ** ** 1.5481.548 로즈마리추출물rosemary extract 26.65226.652 0.20870.2087 ** ** 1.5481.548 허브티추출물herbal tea extract 24.83324.833 0.20870.2087 ** ** 1.5481.548 녹차추출물*로즈마리추출물Green Tea Extract*Rosemary Extract 7.5337.533 1.17591.1759 6.426.42 0.0000.000 1.7181.718 녹차추출물*허브티추출물Green Tea Extract * Herbal Tea Extract -2.158-2.158 1.17591.1759 -1.84-1.84 0.1040.104 1.7181.718 로즈마리추출물*허브티추출물Rosemary Extract*Herbal Tea Extract 0.2730.273 1.17591.1759 0.230.23 0.8220.822 1.7181.718 R-제곱R-squared R-제곱(예측)R-squared (predicted) R-제곱(수정)R-squared (corrected) 86.21%86.21% 72.31%72.31% 77.59%77.59%

출처source DFDF Seq SSSeq SS Adj SSAdj SS Adj MSAdj MS FF PP 회귀분석regression analysis 55 4.536204.53620 4.536204.53620 0.907240.90724 10.0010.00 0.0030.003 선형 linear 22 0.621780.62178 1.544451.54445 0.772230.77223 8.518.51 0.0100.010 2차 Secondary 33 3.914423.91442 3.914423.91442 1.304811.30481 14.3914.39 0.0010.001 녹차추출물*로즈마리추출물 Green Tea Extract*Rosemary Extract 1One 3.608963.60896 3.741993.74199 3.741993.74199 41.2641.26 0.0000.000 녹차추출물*허브티추출물 Green Tea Extract * Herbal Tea Extract 1One 0.300560.30056 0.305460.30546 0.305460.30546 3.373.37 0.1040.104 로즈마리추출물* 허브티추출물 Rosemary Extract* Herbal Tea Extract 1One 0.004900.00490 0.004900.00490 0.004900.00490 0.050.05 0.8220.822 잔차오차residual error 88 0.725540.72554 0.725540.72554 0.090690.09069 적합성 결여 lack of fit 44 0.608290.60829 0.608290.60829 0.152070.15207 5.195.19 0.0700.070 순수오차 pure error 44 0.117250.11725 0.117250.11725 0.029310.02931 총계sum 1313 5.261745.26174

port 계수Coefficient 녹차추출물green tea extract 122.401122.401 로즈마리추출물rosemary extract 118.260118.260 허브티추출물herbal tea extract 124.167124.167 녹차추출물*로즈마리추출물Green Tea Extract*Rosemary Extract 188.834188.834 녹차추출물*허브티추출물Green Tea Extract * Herbal Tea Extract -53.9518-53.9518 로즈마리추출물*허브티추출물Rosemary Extract*Herbal Tea Extract 6.833876.83387

또한, 도 2에 반응궤적도, 도 3에 AOM 값의 혼합물 등고선도 및 표면도, 도 4에 최적화 결과를 나타내었다.In addition, the reaction trajectory diagram in FIG. 2, the contour diagram and surface diagram of the mixture of AOM values in FIG. 3, and the optimization result are shown in FIG.

이에 따라, 본 발명의 유지 산패 억제용 조성물은 유지 전체에 대하여 녹차추출물 0.110 중량% 및 로즈마리추출물 0.0890 중량% 포함될 경우에 AOM 결과가 25.9771 h로 예상되는 점을 확인하였다.Accordingly, it was confirmed that the composition for inhibiting rancidity in oils and fats of the present invention is expected to have an AOM result of 25.9771 h when 0.110 wt% of green tea extract and 0.0890 wt% of rosemary extract are included with respect to the whole fat.

[실시예 1 : 유지 산패 억제용 조성물을 포함하는 유지 조성물 제조][Example 1: Preparation of oil-fat composition comprising composition for inhibiting oil rancidity]

대두유의 전체 중량에 대하여, 녹차추출물 0.110 중량%, 로즈마리추출물 0.089 중량%를 첨가하여 유지 산패 억제용 조성물을 포함하는 유지 조성물을 제조하였다.Based on the total weight of soybean oil, 0.110% by weight of green tea extract and 0.089% by weight of rosemary extract were added to prepare an oil-fat composition comprising a composition for inhibiting oil rancidity.

[실험예 3 : 유지 산패 억제용 조성물을 포함하는 유지의 산패 분석][Experimental Example 3: Analysis of rancidity of oils and fats containing a composition for inhibiting rancidity in oils and fats]

상기 실시예 1에서 제조한 유지 조성물을 이용하여 토르티야(tortilla)를 반복 유탕 처리함에 따른 산패 정도를 분석하였다.Using the oil composition prepared in Example 1, the degree of rancidity due to repeated oil bath treatment of tortillas was analyzed.

토르티야는 토르티야 반죽 전체 중량에 대하여, 중력분 47 중량%, 설탕 5 중량%, 베이킹파우더 1 중량%, 정제수 47 중량%를 혼합 및 반죽하여 제조하였다.The tortilla was prepared by mixing and kneading 47% by weight of flour, 5% by weight of sugar, 1% by weight of baking powder, and 47% by weight of purified water, based on the total weight of the tortilla dough.

토르티야 반죽을 상기 실시예 1에서 제조한 유지 조성물에 넣고 180℃에서 4분간 25회 반복 유탕하였다. 대조군으로는 유지 산패 억제용 조성물을 포함하지 않는 대두유를 이용하였다.The tortilla dough was put in the oil composition prepared in Example 1, and the mixture was repeatedly melted 25 times at 180° C. for 4 minutes. As a control, soybean oil that does not contain a composition for inhibiting oil rancidity was used.

(1) 산화안정도(1) Oxidation stability

산화안정도는 Oxidation tester(873 Biodiesel Rancimat, Metrohm 社)를 이용하여 측정하였다. 유탕 처리 횟수별 AOM 값을 도 5에 나타내었다.Oxidation stability was measured using an oxidation tester (873 Biodiesel Rancimat, Metrohm). The AOM values for each number of times of oil bath are shown in FIG. 5 .

도 5에서 보듯이, 유지 산패 억제용 조성물을 포함하는 유지 조성물을 이용한 유탕 처리의 경우, 대조군에 비해 현저하게 높은 AOM 값을 확인할 수 있었다. 특히, 25회 유탕 처리 한 경우에도, 유탕 처리 전 대조군의 AOM 값에 비해 높은 수치를 보이는 점을 확인하였다.As shown in FIG. 5 , in the case of oil bath treatment using a fat and oil composition including a composition for inhibiting oil rancidity, a significantly higher AOM value than that of the control group could be confirmed. In particular, it was confirmed that even when the oil bath was treated 25 times, the AOM value was higher than the AOM value of the control group before the oil bath treatment.

(2) 지질과산화도(2) lipid peroxidation degree

유탕 처리 횟수별 지질과산화도를 측정하기 위해 Thiobarbituric Acid Reactive Substance (TBARS) test를 수행하였다. Thiobarbituric Acid Reactive Substance (TBARS) test was performed to measure the degree of lipid peroxidation by the number of times of oil bath.

1 %(v/v) 리놀레산과 1 %(v/v) tween 40이 혼합된 에멀젼 0.5ml와 상기 실시예 1에서 제조한 유지 조성물 0.5ml를 혼합하고, 이를 50℃의 수조에서 10시간 반응시켰다. 0.375 %(v/v) TBA(2-thiobarbuturic acid)와 15 %(v/v) TCA(trichloroacetic acid)를 1:1로 혼합한 혼합용액 0.5ml를 첨가하고 100℃에서 15분간 가열한 뒤 10분간 냉각하였다. 이를 10,000 rpm 으로 15분간 원심분리한 뒤, 상층액을 0.1ml씩 96 well plate에 분주하여 532㎚에서 흡광도를 측정하였다.0.5 ml of the emulsion in which 1% (v/v) linoleic acid and 1% (v/v) tween 40 were mixed and 0.5 ml of the oil-fat composition prepared in Example 1 were mixed, and this was reacted in a water bath at 50° C. for 10 hours. . 0.5 ml of a 1:1 mixture of 0.375 % (v/v) TBA (2-thiobarbuturic acid) and 15 % (v/v) TCA (trichloroacetic acid) was added, heated at 100° C. for 15 minutes, and then 10 It was cooled for minutes. After centrifugation at 10,000 rpm for 15 minutes, 0.1 ml of the supernatant was dispensed into 96 well plates and absorbance was measured at 532 nm.

지질과산화(TBARS)값은 아래와 같이 계산하여 도 6에 나타내었다. The lipid peroxidation (TBARS) value was calculated as follows and shown in FIG. 6 .

▶ TBARS (%) = {1-(시험군 흡광도/대조군 흡광도)}×100▶ TBARS (%) = {1-(absorbance of test group / absorbance of control group)}×100

도 6에서 보듯이, 시험군과 대조군 모두 유탕 처리 횟수가 증가함에 따라 지질과산화도가 증가하였으나, 본 발명의 유지 산패 억제용 조성물을 포함하는 유지의 TBARS 값이 대조군에 비해 낮은 값을 보였다.As shown in Figure 6, both the test group and the control group showed an increase in the degree of lipid peroxidation as the number of oil bath treatments increased.

(3) 과산화물가(3) peroxide value

각 횟수별로 반복 유탕 처리된 토르티야를 분쇄하고 에테르에 토르티야 분쇄물이 잠기도록 하여 2시간 방치 후 에테르를 여과하여 채취하였다. 여과된 에테르를 물로 씻어낸 후 감압 농축하여 유지를 회수하였다. 회수한 유지 5g을 취하여 초산과 클로로포름이 3:2 부피비로 혼합된 혼합물 25ml에 녹이고, 포화요오드화칼륨용액 1ml를 가볍게 흔들어 섞은 뒤 어두운 곳에 10분간 방치하였다. 이후, 물 30ml를 가하여 세게 흔들어 섞은 뒤, 전분시액 1ml를 지시약으로 하여 0.01N 티오황산나트륨액으로 적정하였다.The tortillas that had been repeatedly melted for each number of times were crushed, the tortilla crushed product was immersed in ether, and left for 2 hours, and then the ether was filtered and collected. The filtered ether was washed with water and concentrated under reduced pressure to recover the oil. 5 g of the recovered oil was dissolved in 25 ml of a mixture of acetic acid and chloroform in a 3:2 volume ratio, 1 ml of saturated potassium iodide solution was gently shaken, and then left in a dark place for 10 minutes. Thereafter, 30 ml of water was added, shaken vigorously, and titrated with 0.01 N sodium thiosulfate solution using 1 ml of starch solution as an indicator.

과산화물가는 아래와 같이 계산하였으며, 결과값을 도 7에 나타내었다. The peroxide value was calculated as follows, and the results are shown in FIG. 7 .

▶ 과산화물가(meq/kg) = {(a-b)×f}/{검체의 체취량(g)}×10▶ Peroxide value (meq/kg) = {(a-b)×f}/{body odor (g)}×10

a: 0.01N 티오황산나트륨액의 적정수(ml)a: titrated water of 0.01N sodium thiosulfate solution (ml)

b: 공시험에서의 0.01N 티오황산나트륨액의 소비량(ml)b: Consumption of 0.01N sodium thiosulfate solution in blank test (ml)

f: 0.01N 티오황산나트륨액의 역가f: titer of 0.01N sodium thiosulfate solution

도 7에서 보듯이, 시험군과 대조군 모두 유탕 처리 횟수가 증가함에 따라 과산화물가가 증가하였으나, 반복 유탕 처리 20회 이후부터는 본 발명의 유지 산패 억제용 조성물을 포함하는 유지의 과산화물가 값이 대조군에 비해 낮은 값을 보였다.As shown in FIG. 7 , both the test group and the control group increased the peroxide value as the number of oil bath treatments increased, but after 20 repeated oil bath treatments, the peroxide value of the oil and fat containing the composition for inhibiting oil rancidity of the present invention was lower than that of the control group. showed value.

(4) 산가(4) acid value

각 횟수별로 반복 유탕 처리된 토르티야를 분쇄하고 에테르에 토르티야 분쇄물이 잠기도록 하여 2시간 방치 후 에테르를 여과하여 채취하였다. 여과된 에테르를 물로 씻어낸 후 감압 농축하여 유지를 회수하였다. 회수한 유지 10g에 중성의 에탄올과 에테르가 1:2의 부피비로 혼합된 혼합물 100ml를 용해시키고 이를 페놀프탈레인액을 지시약으로 하여 엷은 홍색이 30초간 지속할 때까지 0.1N 에탄올성 KOH로 적정하였다.The tortillas that had been repeatedly melted for each number of times were crushed, the tortilla crushed product was immersed in ether, and left for 2 hours, and then the ether was filtered and collected. The filtered ether was washed with water and concentrated under reduced pressure to recover the oil. In 10 g of the recovered oil and fat, 100 ml of a mixture of neutral ethanol and ether in a volume ratio of 1:2 was dissolved, and titrated with 0.1N ethanolic KOH using phenolphthalein as an indicator until pale red color lasted for 30 seconds.

산가는 아래와 같이 계산하였으며, 결과값을 도 8에 나타내었다.The acid value was calculated as follows, and the results are shown in FIG. 8 .

▶ 산가 (mg/g) = {5.611×(a-b)×f}/S▶ Acid value (mg/g) = {5.611×(a-b)×f}/S

S: 검체의 채취량(g)S: Sample collection amount (g)

a: 검체에 대한 0.1N 에탄올성 KOH의 소비량(ml)a: Consumption of 0.1N ethanolic KOH for the sample (ml)

b: 공시험(에탄올과 에테르 혼합물 1000ml)에 대한 0.1N 에탄올성 KOH의 소비량(ml)b: Consumption (ml) of 0.1N ethanolic KOH for blank test (1000 ml of ethanol and ether mixture)

f: 0.1N 0.1N 에탄올성 KOH의 역가f: titer of 0.1N 0.1N ethanolic KOH

도 8에서 보듯이, 시험군과 대조군 모두 유탕 처리 횟수가 증가함에 따라 산가가 증가하였으나, 반복 유탕 처리 10회 이후부터는 본 발명의 유지 산패 억제용 조성물을 포함하는 유지의 산가 값이 대조군에 비해 낮은 값을 보였다.As shown in Figure 8, both the test group and the control group increased the acid value as the number of oil bath treatment increased, but after 10 repeated oil bath treatment, the acid value of the oil and fat containing the composition for inhibiting oil and fat rancidity of the present invention was lower than that of the control group. showed value.

[실험예 4 : 유지 산패 억제용 조성물을 포함하는 유지를 이용하여 유탕 처리 한 토르티야의 산화안정도 측정][Experimental Example 4: Measurement of oxidative stability of tortillas treated with oil using oil containing a composition for inhibiting oil rancidity]

본 실험은 상기 실험예 3에서 반복 유탕 처리 한 토르티야의 산화안정도(AOM)를 측정하였다. 산화안정도는 Oxidation tester(873 Biodiesel Rancimat, Metrohm 社)를 이용하여 측정하였다. 유탕 처리 횟수별 토르티야의 AOM 값을 도 9에 나타내었다.In this experiment, the oxidation stability (AOM) of the tortillas repeatedly heated in Experimental Example 3 was measured. Oxidation stability was measured using an oxidation tester (873 Biodiesel Rancimat, Metrohm). Fig. 9 shows the AOM values of the tortillas according to the number of times of oil bath.

도 9에서 보듯이, 토르티야의 산화안정도 값은 반복 유탕 처리에 따라 감소하는 경향을 보였으나, 본 발명의 유지 산패 억제용 조성물을 포함하는 유지 조성물을 이용한 유탕 처리의 경우, 대조군을 이용한 경우에 비해 현저하게 높은 AOM 값을 확인할 수 있었다.As shown in Figure 9, the oxidation stability value of the tortilla showed a tendency to decrease with repeated oil bath treatment, but in the case of oil bath treatment using the oil composition comprising the composition for inhibiting oil rancidity of the present invention, compared to the case using the control group. A remarkably high AOM value could be confirmed.

[실시예 2 : 토르티야(tortilla) 제조] [Example 2: Preparation of tortillas]

상기 실시예 1에서 제조한 유지 조성물을 이용하여 토르티야(tortilla) 반죽을 유탕 처리 하고, 대게살을 포함하는 토르티야를 제조하고자 하였다. 토르티야는 토르티야 반죽 전체 중량에 대하여, 중력분 47 중량%, 설탕 5 중량%, 베이킹파우더 1 중량%, 정제수 47 중량%를 혼합 및 반죽하여 제조하였다.Using the oil-fat composition prepared in Example 1, tortilla dough was oiled, and a tortilla containing snow crab meat was prepared. The tortilla was prepared by mixing and kneading 47% by weight of flour, 5% by weight of sugar, 1% by weight of baking powder, and 47% by weight of purified water, based on the total weight of the tortilla dough.

상기 실시예 1에서 제조한 유지 조성물에 토르티야 반죽을 넣고 180℃에서 4분간 유탕 처리하였다. 토르티야 속재료는 속재료 전체 중량에 대하여, 붉은대게살 12.5 중량%, 돼지고기 19 중량%, 소스 25 중량%, 양파 12.5 중량%, 홍새우살 12.5 중량%, 스위트콘 6 중량%, 모짜렐라치즈 12.5 중량%로 구성하였다. 상기 소스는 소스 전체 중량에 대하여, 토마토 스파게티 소스 78.74 중량%, 토마토 케첩 19.69 중량% 및 설탕 1.57 중량%로 구성하였다.The tortilla dough was put into the oil composition prepared in Example 1, and oil bath treatment was performed at 180° C. for 4 minutes. Tortilla ingredients are based on the total weight of ingredients, red snow crab meat 12.5% by weight, pork 19% by weight, sauce 25% by weight, onion 12.5% by weight, red shrimp 12.5% by weight, sweet corn 6% by weight, mozzarella cheese 12.5% by weight was composed of The sauce was composed of 78.74% by weight of tomato spaghetti sauce, 19.69% by weight of tomato ketchup, and 1.57% by weight of sugar, based on the total weight of the sauce.

[실험예 5 : 유지 산패 억제용 조성물을 포함하는 유지를 이용하여 제조한 토르티야의 관능 평가][Experimental Example 5: Sensory evaluation of tortillas prepared using oils and fats containing a composition for inhibiting oil rancidity]

본 실험에서는 상기 실시예 2에서 제조한 속재료를 포함하는 토르티야의 관능평가를 수행하였다. 속재료를 포함하는 토르티야는 170℃에서 9분간 유탕처리하여 조리하였다.In this experiment, sensory evaluation of the tortilla including the filling material prepared in Example 2 was performed. The tortillas containing the ingredients were heated and cooked at 170°C for 9 minutes.

특성별 기호도 관능평가는 20대 성인 20명을 임의로 선발하여, 외관, 내용물양, 맛, 짠맛, 식감, 전체적인 기호도에 대하여 7점 척도법(1:매우 나쁘다, 7:매우 좋다)을 사용하였다. 아래 표 6에 관능평가 결과를 나타내었다.For sensory evaluation of preference for each characteristic, 20 adults in their 20s were randomly selected and a 7-point scale method (1: very bad, 7: very good) was used for appearance, content, taste, saltiness, texture, and overall preference. Table 6 below shows the sensory evaluation results.

속재료를 포함하는 토르티야Tortillas with Ingredients 외관Exterior 5.35 ± 1.185.35 ± 1.18 내용물양content amount 5.45 ± 1.105.45 ± 1.10 taste 5.95 ± 0.835.95 ± 0.83 짠맛salty 5.70 ± 0.985.70 ± 0.98 식감texture 6.35 ± 0.676.35 ± 0.67 전체적인 기호도overall sign 5.80 ± 0.625.80 ± 0.62

표 6은 특성별 기호도 관능평가 결과이다. 외관, 내용물양, 맛, 짠맛은 조금 좋다로 평가되었으며, 식감의 기호도는 좋다로, 전체적인 기호도는 조금 좋다로 평가되었다.Table 6 shows the results of sensory evaluation of preference for each characteristic. Appearance, amount of content, taste, and salty taste were evaluated as slightly good, the taste of texture was evaluated as good, and overall preference was evaluated as slightly good.

또한, 특성별 정도 관능평가는 맛, 짠맛, 식감에 대하여 7점 척도(1:매우 나쁘다, 7:매우 좋다)을 사용하였다. 맛과 짠맛은 1: 매우 약하다, 7: 매우 강하다로 평가하였으며, 식감은 1: 매우 눅눅하다, 7: 매우 바삭하다로 평가하였다.In addition, for sensory evaluation of the degree of each characteristic, a 7-point scale (1: very bad, 7: very good) was used for taste, saltiness, and texture. The taste and saltiness were evaluated as 1: very weak, 7: very strong, and the texture was evaluated as 1: very moist and 7: very crispy.

속재료를 포함하는 토르티야Tortillas with Ingredients 맛의 강도intensity of taste 4.40 ± 0.884.40 ± 0.88 짠맛의 강도saltiness intensity 4.05 ± 0.604.05 ± 0.60 바삭한 정도crispness 5.75 ± 0.795.75 ± 0.79

표 7은 특성별 정도 관능평가 결과이다. 맛과 짠맛의 정도는 약하지도 강하지도 않다로 평가되었으며, 바삭한 정도는 약간 바삭하다로 평가되었다.Table 7 shows the sensory evaluation results for each characteristic. The degree of taste and saltiness was evaluated as neither weak nor strong, and the degree of crispiness was evaluated as slightly crispy.

Claims (7)

녹차추출물과 로즈마리추출물이 0.109~0.111 : 0.088~0.090 의 중량비로 혼합된 혼합물을 포함하며,
상기 녹차 추출물은 녹차를 70%(v/v) 에탄올로 추출한 후 농축 및 동결건조한 분말이며, 상기 로즈마리 추출물은 로즈마리를 70%(v/v) 에탄올로 추출한 후 농축 및 동결건조한 분말이며, 유탕처리 시 유지의 산패를 억제하는 것을 특징으로 하는 유지 산패 억제용 조성물.
Contains a mixture of green tea extract and rosemary extract in a weight ratio of 0.109 to 0.111: 0.088 to 0.090,
The green tea extract is a powder that is concentrated and freeze-dried after extracting green tea with 70% (v/v) ethanol, and the rosemary extract is a powder that is concentrated and freeze-dried after extracting rosemary with 70% (v/v) ethanol, and oil-treated A composition for inhibiting rancidity of oils and fats, characterized in that it suppresses rancidity of city oils and fats.
삭제delete 유지 전체 중량에 대하여, 녹차추출물 0.109~0.111 중량%, 로즈마리추출물 0.088~0.090 중량%이 첨가되며,
상기 녹차 추출물은 녹차를 70%(v/v) 에탄올로 추출한 후 농축 및 동결건조한 분말이며, 상기 로즈마리 추출물은 로즈마리를 70%(v/v) 에탄올로 추출한 후 농축 및 동결건조한 분말인 것을 특징으로 하는 유탕처리용 유지 조성물.
Based on the total weight of oil and fat, 0.109 to 0.111 wt% of green tea extract and 0.088 to 0.090 wt% of rosemary extract are added,
The green tea extract is a powder that is concentrated and freeze-dried after extracting green tea with 70% (v/v) ethanol, and the rosemary extract is a powder that is concentrated and freeze-dried after extracting rosemary with 70% (v/v) ethanol A fat-and-oil composition for oil treatment.
제3항에 있어서,
상기 유지는,
식물성 유지인 것을 특징으로 하는 유탕처리용 유지 조성물.
4. The method of claim 3,
The maintenance is
Oil and fat composition for oil treatment, characterized in that it is vegetable oil.
삭제delete 유지 전체에 대하여, 녹차추출물 0.109~0.111 중량%, 로즈마리추출물 0.088~0.090 중량%를 포함하는 유지 조성물을 제조하는 단계 (a); 및
토르티야(tortilla) 반죽을 상기 유지 조성물에 넣고 유탕처리하는 단계 (b);를 포함하며,
상기 녹차 추출물은 녹차를 70%(v/v) 에탄올로 추출한 후 농축 및 동결건조한 분말이며, 상기 로즈마리 추출물은 로즈마리를 70%(v/v) 에탄올로 추출한 후 농축 및 동결건조한 분말이고,
상기 토르티야 반죽은, 토르티야 반죽 전체 중량에 대하여, 중력분 40~50 중량%, 설탕 1~10 중량%, 베이킹파우더 0.001~2 중량% 및 정제수 40~50 중량%를 혼합한 것이고,
상기 유탕 처리는, 150~200℃에서 5~10분간 유탕처리하는 것을 특징으로 하는 산화안정성이 우수한 토르티야 제조방법.
Based on the whole fat, 0.109 to 0.111 wt% of green tea extract, step (a) of preparing a fat and oil composition comprising 0.088 to 0.090 wt% of rosemary extract; and
Including the; step (b) of putting the tortilla dough into the oil-fat composition and heat-treating it;
The green tea extract is a powder concentrated and freeze-dried after extracting green tea with 70% (v/v) ethanol, and the rosemary extract is a powder concentrated and freeze-dried after extracting rosemary with 70% (v/v) ethanol,
The tortilla dough, based on the total weight of the tortilla dough, 40 to 50% by weight of gravity flour, 1 to 10% by weight of sugar, 0.001 to 2% by weight of baking powder and 40 to 50% by weight of purified water,
The oil bath treatment, a method for producing tortillas excellent in oxidation stability, characterized in that the oil bath treatment at 150 ~ 200 ℃ 5 ~ 10 minutes.
삭제delete
KR1020200159139A 2020-11-24 2020-11-24 Oil composition improved rancidity inhibition and tortilla using the same KR102296932B1 (en)

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