KR102240859B1 - Method for producing steamed and dried Lycium chinense tea using medicinal herb extract - Google Patents

Method for producing steamed and dried Lycium chinense tea using medicinal herb extract Download PDF

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KR102240859B1
KR102240859B1 KR1020200084471A KR20200084471A KR102240859B1 KR 102240859 B1 KR102240859 B1 KR 102240859B1 KR 1020200084471 A KR1020200084471 A KR 1020200084471A KR 20200084471 A KR20200084471 A KR 20200084471A KR 102240859 B1 KR102240859 B1 KR 102240859B1
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bellflower
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권명주
나연호
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나연호
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/10General methods of cooking foods, e.g. by roasting or frying
    • A23L5/13General methods of cooking foods, e.g. by roasting or frying using water or steam
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P20/00Coating of foodstuffs; Coatings therefor; Making laminated, multi-layered, stuffed or hollow foodstuffs
    • A23P20/10Coating with edible coatings, e.g. with oils or fats
    • A23P20/15Apparatus or processes for coating with liquid or semi-liquid products
    • A23P20/18Apparatus or processes for coating with liquid or semi-liquid products by spray-coating, fluidised-bed coating or coating by casting
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/15Flavour affecting agent
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/16Taste affecting agent
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • A23V2200/302Foods, ingredients or supplements having a functional effect on health having a modulating effect on age
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/14Extraction

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
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Abstract

The present invention relates to a method for manufacturing steam-dried bellflower with improved functionality by repeating a process of steaming and drying bellflower by using a medicinal plant extraction liquid, and to steam-dried bellflower manufactured by the method.

Description

약용식물 추출액을 이용한 증포 도라지의 제조방법{Method for producing steamed and dried Lycium chinense tea using medicinal herb extract}Method for producing steamed bellflower using medicinal plant extract {Method for producing steamed and dried Lycium chinense tea using medicinal herb extract}

본 발명은 (1) 물에 청주, 찹쌀 분말 및 결명자를 첨가하여 추출한 후 여과하여 침지액을 제조하는 단계; (2) 도라지를 상기 (1)단계의 제조한 침지액에 침지하여 숙성시킨 후 꺼내어 세척하는 단계; (3) 황칠, 칡, 치자, 로즈힙 및 생강을 혼합하여 약재 혼합물을 준비하는 단계; (4) 상기 (3)단계의 준비한 약재 혼합물에 물을 첨가하여 추출한 후 여과하여 약용식물 열수 추출액을 제조하는 단계; (5) 상기 (4)단계의 제조한 약용식물 추출액을 찜기에 넣고 상기 (2)단계의 세척한 도라지를 찜기의 채반에 올려놓는 단계; 및 (6) 상기 (5)단계의 채반에 놓은 도라지에 상기 (4)단계의 제조한 약용식물 추출액을 분무하면서 찐 후 건조하는 단계를 포함하여 제조하는 것을 특징으로 하는 증포 도라지의 제조방법 및 상기 방법으로 제조된 증포 도라지에 관한 것이다.The present invention comprises the steps of: (1) extracting by adding Cheongju, glutinous rice powder and Cassia tora to water, and then filtering to prepare an immersion solution; (2) immersing the bellflowers in the immersion liquid prepared in step (1), aging them, and taking them out and washing them; (3) preparing a medicinal mixture by mixing hwangchil, arrowroot, gardenia, rosehip and ginger; (4) preparing a hot water extract of medicinal plants by adding water to the herbal mixture prepared in step (3) for extraction, followed by filtering; (5) putting the medicinal plant extract prepared in step (4) in a steamer and placing the washed bellflower in step (2) on a tray of the steamer; And (6) steaming while spraying the extract of the medicinal plant prepared in step (4) on the bellflower placed on the tray of step (5) and drying it. It relates to a thickened bellflower prepared by the method.

도라지는 초롱꽃과의 여러해살이 풀로 산이나 들에서 흔히 자생한다. 높이는 40~100 cm이고 뿌리가 굵으며 줄기는 하나로 나거나 몇 줄기 나오고, 원줄기를 자르면 백색 즙액이 나온다. 잎은 어긋나거나 마주나며 길이가 5~12 cm의 장난형으로 양끝이 좁고 가장자리에 톱니가 있고 표면은 녹색이나 뒷면은 회청색이고 잎자루는 거의 없다. Bellflower is a perennial plant in the family Lanterns and grows naturally in mountains and fields. The height is 40~100 cm, the roots are thick, the stems are one or several stems, and the white juice comes out when the main stem is cut. Leaves are alternate or opposite, 5-12 cm long, playful, narrow at both ends, serrated edges, green on the surface, grayish blue on the back, and few petioles.

꽃은 7~8월에 보라색 또는 백색으로 피는데, 원줄기 끝에 1개 또는 여러 개가 위를 향해 달린다. 화관은 끝이 펴진 종 모양으로 끝이 5갈래로 갈라지고, 꽃받침도 5갈래로 갈라지며, 5개의 수술과 1개의 암술이 있다. 열매는 삭과로 난형이며 꽃받침조각이 달린 채 익는다.Flowers bloom purple or white in July-August, with one or several flowers hanging upwards at the end of the main stem. The corolla is a bell shape with an open end, the end is divided into 5 branches, the calyx is also divided into 5 branches, and has 5 stamens and 1 pistil. Fruits are capsules, ovate, and ripe with calyx pieces attached.

도라지는 우리나라를 비롯해 일본 전역, 중국, 동부 시베리아에 등에서 서식한다. 도라지의 뿌리는 주로 식용으로 이용하고, 한방에서는 도라지를 길경이라고도 하며 신경통과 편도선염 등의 약재로 사용한다. 도라지 뿌리는 사포닌이 많이 포함되어 있어서 생약(桔梗根)으로 이용되고 있다. 그 밖에도 도리지 뿌리에는 칼슘, 섬유질, 철분, 무기질, 단백질, 비타민 등이 함유되어 있다. 한방에서 생약으로 사용되는 도라지는 뿌리가 굵고 내부가 충실하고, 맛이 강한 것이 우량품이다. 주요 효능으로는 거담, 진해, 진통, 진정, 해열 작용이 있으며, 소염진통, 진해거담제로 사용된다. 또한, 콜레스테롤을 저하시키는 효능이 있어 혈관계 질환 및 고혈압에도 효과가 있다. 도라지는 호흡기 질환이라 할 수 있는 감기는 물론 천식에도 탁월한 효능을 보이기 때문에 호흡기 질환에 노출되기 쉬운 면역력이 약한 어린아이나 노인분들, 그리고 잦은 스트레스로 인해서 면역력이 약해진 사람에게 효과가 있다. 도라지는 도라지탕이나 십미패독탕(十味敗毒湯), 방풍통성산(防風通聖散), 배농산(排膿散) 등의 한방재로 사용된다. 우리나라에서 도라지는 뿌리를 김치, 나물, 비빔밥 등의 식재료로 주로 사용하고 최근 들어 정과, 즙액 등의 제품으로 가공하여 이용되고 있다.Bellflower lives in Korea, all over Japan, China, and eastern Siberia. The root of bellflower is mainly used for food, and in oriental medicine, bellflower is also called Gilgyeong, and is used as a medicinal material for neuralgia and tonsillitis. Bellflower root contains a lot of saponin, so it is used as a herbal medicine. In addition, torii roots contain calcium, fiber, iron, minerals, protein, and vitamins. Bellflower, which is used as a herbal medicine in oriental medicine, is a quality product with thick roots, substantial interior, and strong taste. Its main effects include expectorant, antitussive, analgesic, sedative, and antipyretic, and is used as an anti-inflammatory analgesic and antitussive expectorant. In addition, since it has an effect of lowering cholesterol, it is also effective in vascular diseases and high blood pressure. Bellflower has excellent efficacy against asthma as well as colds, which can be called respiratory diseases, so it is effective for children and elderly people with weak immunity who are susceptible to respiratory diseases, and people with weakened immunity due to frequent stress. Bellflower is used for oriental medicines such as Dorajitang, Sipmipaedoktang, Bangpungtongseongsan (防風通聖散) and Baenongsan (排膿散). In Korea, bellflower roots are mainly used as food ingredients such as kimchi, namul, and bibimbap, and recently, they have been processed and used in products such as tablets and juices.

한국등록특허 제2115669에는 벤조피렌의 생성은 억제되면서 사포닌은 증진되고 플라티코딘 D가 저감되는 흑도라지의 제조방법이 개시되어 있고, 한국등록특허 제0807496호에는 황토 지장수를 이용한 홍도라지의 제조방법이 개시되어 있으나, 본 발명의 약용식물 추출액을 이용한 증포 도라지의 제조방법과는 상이하다.Korean Patent No. 2115669 discloses a method for producing black bellflower in which saponin is enhanced and platicodine D is reduced while suppressing the production of benzopyrene. Although disclosed, it is different from the manufacturing method of the thickened bellflower using the medicinal plant extract of the present invention.

본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명에서는 도라지 특유의 이취 및 쓴맛은 제거되면서 기능성 성분 및 항산화 활성이 증진되고 기호도가 우수한 증포 도라지를 제조하기 위해, 도라지 전처리를 위한 재료 선정, 배합비, 증숙 및 건조 조건을 최적화하여, 품질 및 기호도가 우수한 증포 도라지의 제조방법을 제공하는 데 있다.The present invention was derived from the above requirements, and in the present invention, in order to manufacture a thickened bellflower with improved functional ingredients and antioxidant activity and excellent palatability while removing the off-flavor and bitter taste peculiar to bellflower, selecting a material for bellflower pretreatment, It is to provide a method of manufacturing a thickened bellflower with excellent quality and preference by optimizing the mixing ratio, steaming and drying conditions.

상기 과제를 해결하기 위해, 본 발명은 (1) 물에 청주, 찹쌀 분말 및 결명자를 첨가하여 추출한 후 여과하여 침지액을 제조하는 단계; (2) 도라지를 상기 (1)단계의 제조한 침지액에 침지하여 숙성시킨 후 꺼내어 세척하는 단계; (3) 황칠, 칡, 치자, 로즈힙 및 생강을 혼합하여 약재 혼합물을 준비하는 단계; (4) 상기 (3)단계의 준비한 약재 혼합물에 물을 첨가하여 추출한 후 여과하여 약용식물 추출액을 제조하는 단계; (5) 상기 (4)단계의 제조한 약용식물 추출액을 찜기에 넣고 상기 (2)단계의 세척한 도라지를 찜기의 채반에 올려놓는 단계; 및 (6) 상기 (5)단계의 채반에 놓은 도라지에 상기 (4)단계의 제조한 약용식물 추출액을 분무하면서 찐 후 건조하는 단계를 포함하여 제조하는 것을 특징으로 하는 증포 도라지의 제조방법을 제공한다.In order to solve the above problems, the present invention comprises the steps of: (1) adding cheongju, glutinous rice powder, and Cassia tora to water, extracting, and filtering to prepare an immersion solution; (2) immersing the bellflowers in the immersion liquid prepared in step (1), aging them, and taking them out and washing them; (3) preparing a medicinal mixture by mixing hwangchil, arrowroot, gardenia, rosehip and ginger; (4) preparing a medicinal plant extract by adding water to the medicinal mixture prepared in step (3), extracting it, and then filtering it; (5) putting the medicinal plant extract prepared in step (4) in a steamer and placing the washed bellflower in step (2) on a tray of the steamer; And (6) steaming while spraying the medicinal plant extract prepared in step (4) on the bellflower placed on the tray of step (5) and drying it. do.

또한, 본 발명은 상기 방법으로 제조된 증포 도라지를 제공한다.In addition, the present invention provides a thickened bellflower prepared by the above method.

본 발명의 증포 도라지는 기능성 성분 및 항산화 활성이 더욱 증진되어 품질이 우수하며, 도라지 특유의 아린맛과 쓴맛은 제거되고 풍미가 우수하여 기호도가 증진된 증포 도라지를 제공할 수 있는 이점이 있다.The enhancement of functional ingredients and antioxidant activity of the present invention provides excellent quality, and since the peculiar bitter taste and bitter taste of bellflower are removed, and the flavor is excellent, there is an advantage of providing a thickened bellflower with improved palatability.

도 1은 제조예 1과 비교예 1의 증포 도라지의 증숙 및 건조 횟수에 따른 외관 변화를 비교한 사진이다.1 is a photograph comparing the appearance change according to the number of steaming and drying of the thickened bellflowers of Preparation Example 1 and Comparative Example 1. FIG.

본 발명의 목적을 달성하기 위하여, 본 발명은In order to achieve the object of the present invention, the present invention

(1) 물에 청주, 찹쌀 분말 및 결명자를 첨가하여 추출한 후 여과하여 침지액을 제조하는 단계;(1) adding Cheongju, glutinous rice powder, and Cassia tora to water, extracting, and filtering to prepare an immersion solution;

(2) 도라지를 상기 (1)단계의 제조한 침지액에 침지하여 숙성시킨 후 꺼내어 세척하는 단계;(2) immersing the bellflowers in the immersion liquid prepared in step (1), aging them, and taking them out and washing them;

(3) 황칠, 칡, 치자, 로즈힙 및 생강을 혼합하여 약재 혼합물을 준비하는 단계;(3) preparing a medicinal mixture by mixing hwangchil, arrowroot, gardenia, rosehip and ginger;

(4) 상기 (3)단계의 준비한 약재 혼합물에 물을 첨가하여 추출한 후 여과하여 약용식물 추출액을 제조하는 단계;(4) preparing a medicinal plant extract by adding water to the medicinal mixture prepared in step (3), extracting it, and then filtering it;

(5) 상기 (4)단계의 제조한 약용식물 추출액을 찜기에 넣고 상기 (2)단계의 세척한 도라지를 찜기의 채반에 올려놓는 단계; 및(5) putting the medicinal plant extract prepared in step (4) in a steamer and placing the washed bellflower in step (2) on a tray of the steamer; And

(6) 상기 (5)단계의 채반에 놓은 도라지에 상기 (4)단계의 제조한 약용식물 추출액을 분무하면서 찐 후 건조하는 단계를 포함하여 제조하는 것을 특징으로 하는 증포 도라지의 제조방법을 제공한다.(6) It provides a method for producing a thickened bellflower, characterized in that it comprises the step of steaming and drying while spraying the extract of the medicinal plant prepared in the step (4) on the bellflower placed on the tray of the step (5). .

본 발명의 증포 도라지의 제조방법에서, 상기 (1)단계의 침지액은 바람직하게는 물 18~22 L에 청주 45~55 g, 찹쌀 분말 45~55 g 및 결명자 45~55 g을 첨가하여 90~100℃에서 2~4시간 동안 추출한 후 여과하여 제조할 수 있으며, 더욱 바람직하게는 물 20 L에 청주 50 g, 찹쌀 분말 50 g 및 결명자 50 g을 첨가하여 95℃에서 3시간 동안 추출한 후 여과하여 제조할 수 있다. 상기와 같은 조건으로 제조한 침지액은 약리성분 및 기호도가 증진된 도라지 전처리에 적합한 침지액으로 제조할 수 있었다.In the manufacturing method of the enhanced bellflower of the present invention, the immersion liquid in step (1) is preferably 90 to 45 to 55 g of cheongju, 45 to 55 g of glutinous rice powder, and 45 to 55 g of Cassia tora to 18 to 22 L of water. It can be prepared by filtration after extraction at ~100°C for 2 to 4 hours, more preferably 50 g of cheongju, 50 g of glutinous rice powder, and 50 g of Cassia tora are added to 20 L of water, extracted for 3 hours at 95°C, and then filtered. It can be manufactured by doing. The immersion liquid prepared under the above conditions could be prepared as an immersion liquid suitable for pretreatment of bellflower with improved pharmacological components and preference.

또한, 본 발명의 증포 도라지의 제조방법에서, 상기 (2)단계의 숙성은 바람직하게는 25~35℃에서 5~7시간 동안 숙성시킬 수 있으며, 더욱 바람직하게는 30℃에서 6시간 동안 숙성시킬 수 있다. 상기와 같은 조건으로 숙성시킴으로써 도라지 특유의 강한 향과 맛은 제거되고 풍미가 우수하여 기호도를 더욱 증진시킬 수 있었다.In addition, in the manufacturing method of the thickened bellflower of the present invention, the aging of step (2) may be preferably aged at 25 to 35°C for 5 to 7 hours, and more preferably aged at 30°C for 6 hours. I can. By aging under the above conditions, the strong aroma and taste peculiar to bellflowers were removed, and the taste was excellent, so that the preference could be further improved.

또한, 본 발명의 증포 도라지의 제조방법에서, 상기 (3)단계의 약재 혼합물은 바람직하게는 약재 혼합물 총 중량 기준으로, 황칠 58~62 중량%, 칡 18~22 중량%, 치자 8~12 중량%, 로즈힙 4~6 중량% 및 생강 4~6 중량%를 혼합한 것일 수 있으며, 더욱 바람직하게는 약재 혼합물 총 중량 기준으로, 황칠 60 중량%, 칡 20 중량%, 치자 10 중량%, 로즈힙 5 중량% 및 생강 5 중량%를 혼합한 것일 수 있다.In addition, in the manufacturing method of the enlarged bellflower of the present invention, the medicinal mixture of step (3) is preferably 58 to 62 wt% hwangchil, 18 to 22 wt% arrowroot, and 8 to 12 wt% based on the total weight of the medicinal mixture %, rosehip 4-6% by weight and ginger 4-6% by weight may be mixed, more preferably, based on the total weight of the herbal mixture, hwangchil 60% by weight, arrowroot 20% by weight, gardenia 10% by weight, rosehip 5 It may be a mixture of 5% by weight and 5% by weight of ginger.

또한, 본 발명의 증포 도라지의 제조방법에서, 상기 (4)단계의 약용식물 추출액은 바람직하게는 약재 혼합물에 약재 혼합물 대비 물 15~25배(v/w)를 첨가하여 90~100℃에서 2~4시간 동안 추출한 후 여과하여 제조할 수 있으며, 더욱 바람직하게는 약재 혼합물에 약재 혼합물 대비 물 20배(v/w)를 첨가하여 95℃에서 3시간 동안 추출한 후 여과하여 제조할 수 있다.In addition, in the manufacturing method of the enlarged bellflower of the present invention, the medicinal plant extract of step (4) is preferably added to the medicinal mixture by adding 15 to 25 times (v/w) water compared to the medicinal mixture to 2 at 90 to 100°C. It can be prepared by filtration after extracting for ~ 4 hours, more preferably 20 times (v/w) of water compared to the drug mixture is added to the drug mixture, extracted for 3 hours at 95° C., and then filtered.

상기 (3) 및 (4)단계에 거쳐 제조한 약용식물 추출액을 도라지 증숙 시 사용할 경우 도라지의 풍미와 잘 어우러지면서 도라지 특유의 아린맛 및 쓴맛은 제거되고 향미가 증진되어 섭취가 용이할 뿐만 아니라, 항산화 활성이 증진된 도라지로 가공할 수 있었다.When the medicinal plant extract prepared through the above steps (3) and (4) is used for steaming bellflower, it is well harmonized with the flavor of bellflower, while the peculiar bitterness and bitterness of bellflower are removed, and the flavor is enhanced to facilitate intake. It could be processed into bellflower with enhanced antioxidant activity.

또한, 본 발명의 증포 도라지의 제조방법에서, 상기 (5)단계는 바람직하게는 약용식물 추출액 18~22 L를 찜기에 넣고 세척한 도라지 18~22 ㎏을 찜기의 채반에 올려놓을 수 있으며, 더욱 바람직하게는 약용식물 추출액 20 L를 찜기에 넣고 세척한 도라지 20 ㎏을 찜기의 채반에 올려놓을 수 있다.In addition, in the manufacturing method of the enlarged bellflower of the present invention, the step (5) is preferably put 18 to 22 L of medicinal plant extract in a steamer and put 18 to 22 kg of washed bellflower on the tray of the steamer, and further Preferably, 20 L of medicinal plant extract can be put in a steamer and 20 kg of washed bellflower can be placed on a tray of the steamer.

또한, 본 발명의 증포 도라지의 제조방법에서, 상기 (6)단계는 바람직하게는 채반에 놓은 도라지에 약용식물 추출액을 분무하면서 110~130℃에서 증기로 20~40분 동안 찐 후 50~70℃에서 10~14시간 동안 건조하는, 상기 찌고 건조하는 과정을 8~10번 반복할 수 있으며, 더욱 바람직하게는 채반에 놓은 도라지에 약용식물 추출액을 분무하면서 120℃에서 증기로 30분 동안 찐 후 60℃에서 12시간 동안 건조하는, 상기 찌고 건조하는 과정을 9번 반복할 수 있다. 상기와 같은 조건으로 도라지를 찌고 건조함으로써 도라지의 맛을 더욱 무르익게 하고 향기가 더욱 오래 지속되며 부드럽고 깊은맛을 지니는 증포 도라지로 제조할 수 있었다.In addition, in the manufacturing method of the thickened bellflower of the present invention, the step (6) is preferably steamed for 20 to 40 minutes with steam at 110 to 130 °C while spraying the extract of medicinal plants on the bellflower placed on the tray, and then 50 to 70 °C Drying for 10 to 14 hours at, the steaming and drying process can be repeated 8 to 10 times, and more preferably, steamed at 120° C. for 30 minutes with steam while spraying the medicinal plant extract on bellflower placed on a tray and then 60 Drying at °C for 12 hours, the steaming and drying process may be repeated 9 times. By steaming and drying bellflower under the same conditions as described above, the taste of bellflower was ripened, the aroma lasted longer, and it was possible to manufacture a thickened bellflower with a soft and deep taste.

또한, 상기 분무는 도라지를 1회 찔 때마다, 찌기 전에 1번, 찌는 동안 1번, 찐 후 1번, 총 3번 약용식물 추출액을 0.2~0.4 L씩 분무할 수 있으나, 이에 제한되지 않는다.In addition, the spraying may spray 0.2 to 0.4 L of medicinal plant extract each time, once before steaming, once before steaming, once during steaming, once after steaming, and three times in total, but is not limited thereto.

본 발명의 증포 도라지의 제조방법은, 보다 구체적으로는The manufacturing method of the thickened bellflower of the present invention, more specifically

(1) 물 18~22 L에 청주 45~55 g, 찹쌀 분말 45~55 g 및 결명자 45~55 g을 첨가하여 90~100℃에서 2~4시간 동안 추출한 후 여과하여 침지액을 제조하는 단계;(1) Step of adding 45-55 g of Cheongju, 45-55 g of glutinous rice powder, and 45-55 g of Cassia tora to 18-22 L of water, extracting for 2-4 hours at 90-100°C, and filtering to prepare an immersion solution ;

(2) 도라지를 상기 (1)단계의 제조한 침지액에 침지하여 25~35℃에서 5~7시간 동안 숙성시킨 후 꺼내어 세척하는 단계;(2) immersing bellflower in the immersion solution prepared in step (1), aging at 25 to 35°C for 5 to 7 hours, then taking out and washing;

(3) 약재 혼합물 총 중량 기준으로, 황칠 58~62 중량%, 칡 18~22 중량%, 치자 8~12 중량%, 로즈힙 4~6 중량% 및 생강 4~6 중량%를 혼합하여 약재 혼합물을 준비하는 단계;(3) Based on the total weight of the herbal mixture, a mixture of 58 to 62% by weight of hwangchil, 18 to 22% by weight of arrowroot, 8 to 12% by weight of gardenia, 4 to 6% by weight of rosehip, and 4 to 6% by weight of ginger, Preparing;

(4) 상기 (3)단계의 준비한 약재 혼합물에 약재 혼합물 대비 물 15~25배(v/w)를 첨가하여 90~100℃에서 2~4시간 동안 추출한 후 여과하여 약용식물 추출액을 제조하는 단계; (4) preparing a medicinal plant extract by adding 15 to 25 times (v/w) water compared to the medicinal mixture to the medicinal mixture prepared in step (3), extracting at 90 to 100°C for 2 to 4 hours, and then filtering ;

(5) 상기 (4)단계의 제조한 약용식물 추출액 18~22 L를 찜기에 넣고 상기 (2)단계의 세척한 도라지 18~22 ㎏을 찜기의 채반에 올려놓는 단계; 및(5) placing 18-22 L of the medicinal plant extract prepared in step (4) into a steamer and placing 18-22 kg of washed bellflower in step (2) on a tray of the steamer; And

(6) 상기 (5)단계의 채반에 놓은 도라지에 상기 (4)단계의 제조한 약용식물 추출액을 분무하면서 110~130℃에서 증기로 20~40분 동안 찐 후 50~70℃에서 10~14시간 동안 건조하는, 상기 찌고 건조하는 과정을 8~10번 반복하는 단계를 포함할 수 있으며,(6) While spraying the medicinal plant extract prepared in step (4) on the bellflower placed on the tray of step (5), steam at 110 to 130°C for 20 to 40 minutes with steam, and then at 50 to 70°C for 10 to 14 Drying for a period of time, may include the step of repeating the steaming and drying process 8 to 10 times,

더욱 구체적으로는More specifically

(1) 물 20 L에 청주 50 g, 찹쌀 분말 50 g 및 결명자 50 g을 첨가하여 95℃에서 3시간 동안 추출한 후 여과하여 침지액을 제조하는 단계;(1) adding 50 g of cheongju, 50 g of glutinous rice and 50 g of Cassia tora to 20 L of water, extracting at 95°C for 3 hours, and filtering to prepare an immersion solution;

(2) 도라지를 상기 (1)단계의 제조한 침지액에 침지하여 30℃에서 6시간 동안 숙성시킨 후 꺼내어 세척하는 단계;(2) immersing the bellflower in the immersion solution prepared in step (1), aging at 30°C for 6 hours, taking out and washing;

(3) 약재 혼합물 총 중량 기준으로, 황칠 60 중량%, 칡 20 중량%, 치자 10 중량%, 로즈힙 5 중량% 및 생강 5 중량%를 혼합하여 약재 혼합물을 준비하는 단계;(3) preparing a herbal mixture by mixing 60% by weight of hwangchil, 20% by weight of arrowroot, 10% by weight of gardenia, 5% by weight of rosehip and 5% by weight of ginger, based on the total weight of the herbal mixture;

(4) 상기 (3)단계의 준비한 약재 혼합물에 약재 혼합물 대비 물 20배(v/w)를 첨가하여 95℃에서 3시간 동안 추출한 후 여과하여 약용식물 추출액을 제조하는 단계;(4) preparing a medicinal plant extract by adding 20 times (v/w) water compared to the medicinal mixture to the medicinal mixture prepared in step (3), extracting at 95° C. for 3 hours, and filtering;

(5) 상기 (4)단계의 제조한 약용식물 추출액 20 L를 찜기에 넣고 상기 (2)단계의 세척한 도라지 20 ㎏을 찜기의 채반에 올려놓는 단계; 및(5) putting 20 L of the medicinal plant extract prepared in step (4) into a steamer and placing 20 kg of the washed bellflower in step (2) on a tray of the steamer; And

(6) 상기 (5)단계의 채반에 놓은 도라지에 상기 (4)단계의 제조한 약용식물 추출액을 분무하면서 120℃에서 증기로 30분 동안 찐 후 60℃에서 12시간 동안 건조하는, 상기 찌고 건조하는 과정을 9번 반복하는 단계를 포함할 수 있다.(6) While spraying the medicinal plant extract prepared in step (4) on the bellflower placed on the tray of step (5), steaming for 30 minutes with steam at 120°C and drying at 60°C for 12 hours, the steaming and drying It may include a step of repeating the process 9 times.

본 발명은 또한, 상기 방법으로 제조된 증포 도라지를 제공한다.The present invention also provides a thickened bellflower prepared by the above method.

이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

제조예 1. 증포 도라지 제조Preparation Example 1. Preparation of thickened bellflower

(1) 겨울동안 채취한 3~5년생 도라지 뿌리를 흐르는 물에 3회 이상 깨끗하게 세척하고, 세척한 도라지 중 몸통이 1.5~2.5 ㎝ 이상의 모양이 우수한 도라지를 선별하여 준비하였다.(1) Three to five year old bellflower roots collected during the winter were washed three or more times in running water, and among the washed bellflowers, bellflowers with an excellent shape of 1.5 to 2.5 cm or more were selected and prepared.

(2) 정제수 20 L에 청주 50 g, 찹쌀 분말 50 g 및 결명자 50 g을 첨가하여 95℃에서 3시간 동안 추출한 후 여과하여 침지액을 제조하였다.(2) 50 g of cheongju, 50 g of glutinous rice powder and 50 g of Cassia tora were added to 20 L of purified water, extracted for 3 hours at 95°C, and filtered to prepare an immersion solution.

(3) 상기 (1)단계의 준비한 도라지를 상기 (2)단계의 제조한 침지액에 침지하여 30℃에서 6시간 동안 숙성시킨 후 꺼내어 흐르는 물에 세척하였다.(3) The bellflower prepared in step (1) was immersed in the immersion solution prepared in step (2), aged at 30°C for 6 hours, and then taken out and washed with running water.

(4) 황칠 잎과 줄기를 잘 건조하고 0.5~1 ㎝ 크기로 파쇄하였다. 건조한 칡뿌리와 치자를 0.5~1 ㎝ 정도의 크기로 파쇄하였고, 생강을 0.3~0.5 ㎝ 정도의 두께로 절단하여 60℃에서 10시간 건조하였다. 약재 혼합물 총 중량 기준으로, 상기 파쇄한 황칠 60 중량%, 칡 20 중량% 및 치자 10 중량%와 건조한 생강 5 중량% 및 로즈힙 열매 5 중량%를 혼합한 약재 혼합물에 약재 혼합물 대비 정제수 20배(v/w)를 첨가하여 95℃에서 3시간 동안 추출한 후 2회 이상 여과하여 약용식물 열수 추출액을 제조하였다.(4) Hwangchil leaves and stems were well dried and crushed into 0.5~1 ㎝ size. Dried arrowroot and gardenia were crushed to a size of about 0.5 to 1 cm, and ginger was cut into a thickness of about 0.3 to 0.5 cm and dried at 60°C for 10 hours. Based on the total weight of the drug mixture, the crushed hwangchil 60% by weight, arrowroot 20% by weight, and 10% by weight of gardenia, 5% by weight of dried ginger and 5% by weight of rosehip fruit were mixed in a mixture of 20 times purified water compared to the drug mixture (v /w) was added and extracted for 3 hours at 95°C, and then filtered twice or more to prepare a hot water extract of medicinal plants.

(5) 상기 (4)단계의 제조한 약용식물 열수 추출액 20 L를 찜기에 넣고 상기 (3)단계의 세척한 도라지 20 ㎏을 찜기의 채반에 깔고 찜기의 뚜껑을 닫아 120℃의 온도에서 증기로 30분간 쪄낸다. 이때 도라지를 찜기에 넣고 찌기 전에 1번, 찌는 동안 1번, 찐 후 1번 총 3번 약용식물 열수 추출액 0.3 L씩 분무하였다. 상기 쪄낸 도라지를 60℃ 건조기에서 12시간 동안 건조하였다. 상기 도라지를 찌고 건조하는 과정을 총 9번 반복하여 증포 도라지를 제조하였다.(5) Put 20 L of the hot water extract of medicinal plants prepared in step (4) into a steamer, spread 20 kg of the washed bellflower in step (3) on a tray of the steamer, close the lid of the steamer to steam at a temperature of 120°C. Steam for 30 minutes. At this time, the bellflower was put in a steamer and sprayed with 0.3 L of hot water extract of medicinal plants 1 time before steaming, 1 time during steaming, and 1 time after steaming. The steamed bellflower was dried in a dryer at 60° C. for 12 hours. The process of steaming and drying the bellflower was repeated a total of 9 times to prepare a thickened bellflower.

재료 종류Material type 재료 종류Material type 제조예 1Manufacturing Example 1 비교예 1Comparative Example 1 비교예 2Comparative Example 2 비교예 3Comparative Example 3 침지액(g)Immersion liquid (g) 청주Rice wine 5050 -- 150150 3030 찹쌀 분말Glutinous rice powder 5050 -- -- 3030 결명자Cassia 5050 -- -- 9090 약용식물 열수 추출액(%)Medicinal plant hot water extract (%) 황칠Hwangchil 6060 -- 6060 3030 Arrowroot 2020 -- 3030 4545 치자Gardenia 1010 -- -- 55 생강ginger 55 -- 1010 1010 로즈힙Rose hip 55 -- -- 1010

비교예 1. 증포 도라지 제조Comparative Example 1. Preparation of thickened bellflower

(1) 겨울동안 채취한 3~5년생 도라지 뿌리를 흐르는 물에 3회 이상 깨끗하게 세척하고, 세척한 도라지 중 몸통이 1.5~2.5 ㎝ 이상의 모양이 우수한 도라지를 선별하여 준비하였다.(1) Three to five year old bellflower roots collected during the winter were washed three or more times in running water, and among the washed bellflowers, bellflowers with an excellent shape of 1.5 to 2.5 cm or more were selected and prepared.

(2) 정제수 20 L를 찜기에 넣고 상기 (1)단계의 준비한 도라지 20 ㎏을 찜기의 채반에 깔고 찜기의 뚜껑을 닫아 120℃의 온도에서 증기로 30분간 쪄낸다. 상기 쪄낸 도라지를 60℃ 건조기에서 12시간 동안 건조하였다. 상기 도라지를 찌고 건조하는 과정을 총 9번 반복하여 증포 도라지를 제조하였다.(2) Put 20 L of purified water in a steamer, spread 20 kg of bellflowers prepared in step (1) on a tray of the steamer, close the lid of the steamer, and steam for 30 minutes with steam at a temperature of 120℃. The steamed bellflower was dried in a dryer at 60° C. for 12 hours. The process of steaming and drying the bellflower was repeated a total of 9 times to prepare a thickened bellflower.

비교예 2 및 3. 증포 도라지 제조Comparative Examples 2 and 3. Preparation of thickened bellflower

상기 제조예 1의 방법으로 증포 도라지를 제조하되, (2)단계의 침지액 및 (4)단계의 약용식물 열수 추출액 제조 시 부재료 첨가량을 상기 표 1의 배합비로 배합한 침지액 및 약용식물 열수 추출액을 이용하여, 비교예 2 및 3의 증포 도라지를 각각 제조하였다.In the case of preparing the thickened bellflower by the method of Preparation Example 1, the immersion liquid in step (2) and the hot water extract of medicinal plants in step (4) are mixed in the mixing ratio of Table 1 when preparing the immersion liquid in step (2) and the hot water extract of medicinal plants Using, the thickened bellflowers of Comparative Examples 2 and 3 were prepared, respectively.

비교예 4. 증포 도라지 제조Comparative Example 4. Preparation of thickened bellflower

(1) 겨울동안 채취한 3~5년생 도라지 뿌리를 흐르는 물에 3회 이상 깨끗하게 세척하고, 세척한 도라지 중 몸통이 1.5~2.5 ㎝ 이상의 모양이 우수한 도라지를 선별하여 준비하였다.(1) Three to five year old bellflower roots collected during the winter were washed three or more times in running water, and among the washed bellflowers, bellflowers with an excellent shape of 1.5 to 2.5 cm or more were selected and prepared.

(2) 정제수 20 L에 청주 50 g, 찹쌀 분말 50 g 및 결명자 50 g을 첨가하여 95℃에서 1시간 동안 추출한 후 여과하여 침지액을 제조하였다.(2) 50 g of cheongju, 50 g of glutinous rice powder and 50 g of Cassia tora were added to 20 L of purified water, extracted for 1 hour at 95°C, and filtered to prepare an immersion solution.

(3) 상기 (1)단계의 준비한 도라지를 상기 (2)단계의 제조한 침지액에 침지하여 20℃에서 12시간 동안 숙성시킨 후 꺼내어 흐르는 물에 세척하였다.(3) The bellflower prepared in step (1) was immersed in the immersion solution prepared in step (2), aged at 20°C for 12 hours, and then taken out and washed with running water.

(4) 황칠 잎과 줄기를 잘 건조하고 0.5~1 ㎝ 크기로 파쇄하였다. 건조한 칡뿌리와 치자를 0.5~1 ㎝ 정도의 크기로 파쇄하였고, 생강을 0.3~0.5 ㎝ 정도의 두께로 절단하여 60℃에서 10시간 건조하였다. 약재 혼합물 총 중량 기준으로, 상기 파쇄한 황칠 60 중량%, 칡 20 중량% 및 치자 10 중량%와 건조한 생강 5 중량% 및 로즈힙 열매 5 중량%를 혼합한 약재 혼합물에 약재 혼합물 대비 정제수 20배(v/w)를 첨가하여 95℃에서 6시간 동안 추출한 후 2회 이상 여과하여 약용식물 열수 추출액을 제조하였다.(4) Hwangchil leaves and stems were well dried and crushed into 0.5~1 ㎝ size. Dried arrowroot and gardenia were crushed to a size of about 0.5 to 1 cm, and ginger was cut into a thickness of about 0.3 to 0.5 cm and dried at 60°C for 10 hours. Based on the total weight of the drug mixture, the crushed hwangchil 60% by weight, arrowroot 20% by weight, and 10% by weight of gardenia, 5% by weight of dried ginger and 5% by weight of rosehip fruit were mixed in a mixture of 20 times purified water compared to the drug mixture (v /w) was added and extracted for 6 hours at 95°C, and then filtered twice or more to prepare a hot water extract of medicinal plants.

(5) 상기 (4)단계의 제조한 약용식물 열수 추출액 20 L를 찜기에 넣고 상기 (3)단계의 세척한 도라지 20 ㎏을 찜기의 채반에 깔고 찜기의 뚜껑을 닫아 100℃의 온도에서 증기로 50분간 쪄낸다. 이때 도라지를 찜기에 넣고 찌기 전에 1번, 찌는 동안 1번, 찐 후 1번 총 3번 약용식물 열수 추출액 0.3 L씩 분무하였다. 상기 쪄낸 도라지를 40℃ 건조기에서 6시간 동안 건조하였다. 상기 도라지를 찌고 건조하는 과정을 총 9번 반복하여 증포 도라지를 제조하였다.(5) Put 20 L of the hot water extract of medicinal plants prepared in step (4) into a steamer, spread 20 kg of the washed bellflower in step (3) on a tray of the steamer, close the lid of the steamer, and use steam at a temperature of 100°C. Steam for 50 minutes. At this time, the bellflower was put in a steamer and sprayed with 0.3 L of hot water extract of medicinal plants 1 time before steaming, 1 time during steaming, and 1 time after steaming. The steamed bellflower was dried in a dryer at 40° C. for 6 hours. The process of steaming and drying the bellflower was repeated a total of 9 times to prepare a thickened bellflower.

실시예 1: 총 페놀화합물 함량 측정Example 1: Measurement of total phenolic compound content

총 페놀화합물 함량은 폴린-데니스(Folin-Denis) 방법에 따라 분석하였다. 제조된 시료를 각각 열수 추출하여 1 ㎎/㎖로 조제한 후, 이 시료액 1 mL에 증류수 3 mL를 넣고 Folin & Ciocalteau's 페놀 시약 1 mL를 첨가한 후 27℃ 쉐이킹배스(Shaking bath)에서 혼합하였다. 5분 후 NaCO3 포화용액 1 mL를 넣고 혼합하여 실온에서 1시간 방치한 후 640 nm에서 분광광도계(UV-1650PC, SHIMADZU)로 흡광도를 측정하였다. 페놀화합물 함량은 카테킨, 클로로겐산 및 탄닌산의 농도를 이용하여 검량선을 작성한 다음 정량하였다.The total phenolic compound content was analyzed according to the Folin-Denis method. Each prepared sample was extracted with hot water to prepare 1 mg/ml, and then 3 mL of distilled water was added to 1 mL of this sample solution, and 1 mL of Folin &Ciocalteau's phenol reagent was added, followed by mixing in a 27°C shaking bath. After 5 minutes, 1 mL of a saturated NaCO 3 solution was added, mixed, and allowed to stand at room temperature for 1 hour, and the absorbance was measured at 640 nm with a spectrophotometer (UV-1650PC, SHIMADZU). The content of phenol compounds was quantified after preparing a calibration curve using the concentrations of catechin, chlorogenic acid, and tannic acid.

증포 도라지의 총 페놀화합물 함량Total phenolic compounds content of thickened bellflower 시료sample 총 페놀 함량(ppm)Total phenol content (ppm) 카테킨Catechin 클로로겐산Chlorogenic acid 탄닌산Tannic acid 제조예 1Manufacturing Example 1 186.9±3.0186.9±3.0 289.8±4.5289.8±4.5 198.9±3.0198.9±3.0 비교예 1Comparative Example 1 90.4±2.690.4±2.6 181.0±2.3181.0±2.3 102.4±2.6102.4±2.6 비교예 2Comparative Example 2 105.3±3.2105.3±3.2 202.8±2.8202.8±2.8 126.3±1.5126.3±1.5 비교예 3Comparative Example 3 114.3±0.9114.3±0.9 237.4±4.8237.4±4.8 141.5±3.3141.5±3.3 비교예 4Comparative Example 4 148.3±1.0148.3±1.0 252.0±3.5252.0±3.5 165.3±1.0165.3±1.0

증포 도라지의 총 페놀화합물 함량을 상기 표 2에 나타내었다. 표 2에서 알 수 있는 바와 같이, 표준물질 클로로겐산을 기준으로 정량한 결과 제조예 1이 가장 높은 함량을 나타내었고, 비교예 1이 가장 낮은 함량을 나타내었다. 이는 카테킨 및 탄닌산을 기준으로 정량한 결과에서도 제조예 1의 증포 도라지가 가장 높은 함량을 나타내었다.Table 2 shows the total phenolic compound content of the thickened bellflower. As can be seen in Table 2, as a result of quantification based on the standard chlorogenic acid, Preparation Example 1 showed the highest content, and Comparative Example 1 showed the lowest content. This was also the result of quantification based on catechin and tannic acid showed the highest content of the thickened bellflower of Preparation Example 1.

실시예 2: 총 플라보노이드 함량Example 2: Total flavonoid content

총 플라보노이드 함량 측정은 각각의 시료 추출물 1.0 mL를 시험관에 취하고 10 mL의 디에틸렌 글리콜(diethylen glycol)을 가하여 잘 혼합하였다. 다시 여기에 1N NaOH 0.1 mL를 잘 혼합시켜 37℃의 항온수조(water bath)에서 1시간 동안 반응시킨 후 420 nm에서 흡광도를 측정하였다. 공시험은 시료 용액 대신 50% 메탄올(methanol) 용액을 동일하게 처리하며, 표준곡선은 나린진(Sigma Co., USA)을 이용하여 작성하고 이로부터 총 플라보노이드 함량을 구하였다.To measure the total flavonoid content, 1.0 mL of each sample extract was taken into a test tube, and 10 mL of diethylen glycol was added and mixed well. Again, 0.1 mL of 1N NaOH was well mixed and reacted in a water bath at 37° C. for 1 hour, and the absorbance was measured at 420 nm. In the blank test, a 50% methanol solution was treated in the same manner instead of the sample solution, and a standard curve was prepared using Narinjin (Sigma Co., USA), and the total flavonoid content was calculated from this.

증포 도라지의 총 플라보노이드 함량Total Flavonoid Content of Thickened Bellflower 시료sample 총 플라보노이드 함량(ppm)Total flavonoid content (ppm) 제조예 1Manufacturing Example 1 53.0±1.253.0±1.2 비교예 1Comparative Example 1 22.9±1.122.9±1.1 비교예 2Comparative Example 2 25.7±1.225.7±1.2 비교예 3Comparative Example 3 29.4±1.029.4±1.0 비교예 4Comparative Example 4 34.9±2.834.9±2.8

제조예 1과 비교예들의 증포 도라지의 플라보노이드 함량을 측정한 결과는 상기 표 3에 나타내었다. 표 3에서 알 수 있는 바와 같이, 도라지를 침지액에 숙성, 약용식물 추출액으로 처리하지 않은 비교예 1이 가장 낮음 함량을 나타내었고, 제조예 1의 증포 도라지가 플라보노이드 함량이 높은 것으로 나타났다.The results of measuring the flavonoid content of the thickened bellflowers of Preparation Example 1 and Comparative Examples are shown in Table 3 above. As can be seen in Table 3, bellflowers ripening in immersion solution, Comparative Example 1 not treated with a medicinal plant extract showed the lowest content, and the thickened bellflower of Preparation Example 1 was found to have a high flavonoid content.

실시예 3: DPPH 라디칼 소거능Example 3: DPPH radical scavenging activity

항산화능을 알아보기 위해 수소전자공여능에 의해 항산화 활성을 측정하였다. 여러 농도의 시료를 증류수로 희석하여, 900 ㎕의 DPPH 용액(100 uM)과 각 시료 100 ㎕를 혼합하여 교반하였다. 이 혼합 시료를 암소에서 30분간 반응시킨 후 517 nm에서 흡광도를 측정하였다. 수소전자공여능은 각 실험을 3회 반복하여 평균을 낸 다음 대조구에 대한 흡광도의 감소 정도를 다음 식에 의하여 계산하였다.To determine the antioxidant activity, the antioxidant activity was measured by the hydrogen electron donating ability. Samples of various concentrations were diluted with distilled water, and 900 µl of DPPH solution (100 uM) and 100 µl of each sample were mixed and stirred. The mixed sample was reacted in the dark for 30 minutes, and then absorbance was measured at 517 nm. The hydrogen electron donating ability was averaged by repeating each experiment three times, and then the degree of decrease in absorbance for the control was calculated by the following equation.

An = (A0-A)/A0 × 100An = (A 0 -A)/A 0 × 100

An: DPPH 라디칼 소거능에 대한 항산화 활성(%)An: Antioxidant activity against DPPH radical scavenging activity (%)

A0: 시료가 첨가되지 않은 DPPH 용액의 흡광도A 0 : Absorbance of DPPH solution without sample added

A: 반응용액 중의 DPPH와 시료의 반응한 흡광도A: absorbance of the sample with DPPH in the reaction solution

증포 도라지의 DPPH 라디칼 소거능(%)DPPH radical scavenging activity of thickened bellflower (%) 시료sample DPPH 라디칼 소거능(%) DPPH radical scavenging activity (%) 50005000 25002500 12501250 제조예 1Manufacturing Example 1 71.8±0.371.8±0.3 56.2±0.656.2±0.6 35.1±1.035.1±1.0 비교예 1Comparative Example 1 50.9±0.350.9±0.3 31.5±0.731.5±0.7 19.9±1.319.9±1.3 비교예 2Comparative Example 2 53.0±0.653.0±0.6 37.7±1.137.7±1.1 24.9±1.224.9±1.2 비교예 3Comparative Example 3 58.2±0.958.2±0.9 42.7±1.042.7±1.0 26.6±0.626.6±0.6 비교예 4Comparative Example 4 63.4±0.963.4±0.9 49.1±0.249.1±0.2 28.0±0.828.0±0.8

증포 도라지를 희석하여 DPPH 라디칼 소거능 결과는 상기 표 4에 나타내었다. 그 결과, 5000 ppm 농도에서 비교예 1의 무처리 증포 도라지에 비해 제조예 1의 증포 도라지가 높은 DPPH 라디칼 소거능을 나타내었다. 비교예들 중에서는 비교예 4의 증포 도라지가 가장 높은 소거능을 나타내었다.The results of DPPH radical scavenging ability by diluting the thickened bellflower are shown in Table 4 above. As a result, at a concentration of 5000 ppm, the thickened bellflower of Preparation Example 1 showed higher DPPH radical scavenging ability compared to the untreated thickened bellflower of Comparative Example 1. Among the comparative examples, the thickened bellflower of Comparative Example 4 exhibited the highest scavenging ability.

실시예 4: 아질산염 소거능 시험Example 4: Nitrite scavenging ability test

항산화능을 알아보기 위한 또 다른 방법의 하나로서 아질산염 소거작용의 측정은 1 mM NaNO2 20 ㎕에 1/10의 농도로 희석한 시료 40 ㎕와 0.1N HCl(pH 1.2) 또는 0.2M 구연산염 버퍼(citrate buffer, pH 4.2) 또는 0.2M 구연산염 버퍼(citrate buffer, pH 6.0) 140 ㎕를 사용하여 부피를 200 ㎕로 맞추었다. 이 반응액을 37℃ 항온 수조에서 1시간 반응시킨 후 2% 아세트산 1000 ㎕, Griess 시약(30% 아세트산으로 조제한 1% 설파닐산(sulfanilic acid)과 1% 나프틸아민(naphthylamine)을 1:1 비율로 혼합한 것, 사용 직전에 조제) 80 ㎕를 가하여 잘 혼합하고 빛을 차단한 상온에서 15분간 반응시킨 후 520 nm에서 흡광도를 측정하여 아래와 같이 아질산염 소거능을 구하였다.As another method for determining the antioxidant activity, the measurement of nitrite scavenging activity is 40 µl of a sample diluted to a concentration of 1/10 in 20 µl of 1 mM NaNO 2 and 0.1N HCl (pH 1.2) or 0.2M citrate buffer ( citrate buffer, pH 4.2) or 0.2M citrate buffer (citrate buffer, pH 6.0) 140 µl was used to adjust the volume to 200 µl. After reacting the reaction solution in a constant temperature water bath at 37°C for 1 hour, 1000 µl of 2% acetic acid, 1% sulfanilic acid prepared with 30% acetic acid and 1% naphthylamine in a 1:1 ratio Mixture with, prepared immediately before use) 80 µl was added, mixed well, reacted for 15 minutes at room temperature where light was blocked, and absorbance was measured at 520 nm to obtain the nitrite scavenging ability as follows.

N(%) = [1-(A-C)/B]×100N(%) = [1-(A-C)/B]×100

N: 아질산염 소거능(nitrite scavenging ability)N: nitrite scavenging ability

A: 시료에 1mM NaNO2를 첨가하여 1시간 동안 반응시킨 후 흡광도A: Absorbance after adding 1mM NaNO 2 to the sample and reacting for 1 hour

B: 1mM NaNO2 흡광도B: 1mM NaNO 2 absorbance

C: 대조구 흡광도C: control absorbance

증포 도라지의 아질산염 소거능(%)Nitrite scavenging ability of thickened bellflower (%) 시료sample 아질산염 소거능(%)Nitrite scavenging ability (%) 제조예 1Manufacturing Example 1 41.8±2.641.8±2.6 비교예 1Comparative Example 1 25.1±0.525.1±0.5 비교예 2Comparative Example 2 30.3±4.830.3±4.8 비교예 3Comparative Example 3 32.8±1.632.8±1.6 비교예 4Comparative Example 4 36.6±1.436.6±1.4

증포 도라지 추출 시료를 희석하여 아질산염 소거능 결과는 상기 표 5에 나타내었다. 그 결과, 표 5에서 알 수 있는 바와 같이, 제조예 1의 증포 도라지가 41.8%의 아질산염 소거능을 나타내어 가장 높은 활성을 나타내었고, 비교예 1이 25.1%로 가장 낮은 소거능을 나타냄을 확인할 수 있었다.The results of nitrite scavenging ability by diluting the thickened bellflower extract sample are shown in Table 5 above. As a result, as can be seen in Table 5, the thickened bellflower of Preparation Example 1 exhibited a nitrite scavenging ability of 41.8%, showing the highest activity, and Comparative Example 1 showed the lowest scavenging ability at 25.1%.

실시예 5. 조사포닌 함량Example 5. Content of irradiation ponins

총 사포닌의 분석은 각 도라지 추출액 100 ㎕를 정확히 취하여 시험관에 넣고 얼음물 속에서 8% 바닐린-에탄올 용액 0.3 mL과 72% 황산 용액 4 mL를 가하였다. 시험관을 60℃ 항온수조에 넣고 10분간 가온하여 내용액을 발색시킨 후 545 nm에서 흡광도를 측정하였다. 대조구는 농도별로 조제한 ginsenoside-Re 표준용액 각 100 ㎕를 정확히 취하여 시료와 동일한 방법으로 발색시킨 후 분광도를 측정하여 검량선을 작성하고 시료에 함유되어 있는 총 사포닌 함량을 구하였다.For the analysis of total saponin, 100 μl of each bellflower extract was accurately taken and placed in a test tube, and 0.3 mL of 8% vanillin-ethanol solution and 4 mL of 72% sulfuric acid solution were added in ice water. The test tube was placed in a constant temperature water bath at 60° C. and heated for 10 minutes to develop the solution, and the absorbance was measured at 545 nm. As a control, 100 µl each of the ginsenoside-Re standard solution prepared for each concentration was accurately taken and colored in the same manner as the sample, and then the spectral diagram was measured to prepare a calibration curve, and the total saponin content contained in the sample was calculated.

증포 도라지의 조사포닌 함량The content of irradiated ponins in the thickened bellflower 시료sample 조사포닌 함량(ppm)Irradiation ponin content (ppm) 제조예 1Manufacturing Example 1 36.2±0.936.2±0.9 비교예 1Comparative Example 1 23.9±0.623.9±0.6 비교예 2Comparative Example 2 27.5±3.227.5±3.2 비교예 3Comparative Example 3 28.8±1.228.8±1.2 비교예 4Comparative Example 4 32.3±0.832.3±0.8

증포 도라지의 조사포닌 함량을 측정한 결과는 상기 표 6에 나타내었다. 그 결과, 비교예들에 비해 제조예 1의 증포 도라지가 상대적으로 높은 사포닌 함량을 나타내었다. 따라서, 제조예 1의 조건으로 도라지를 전처리하여 증포 도라지를 제조하는 것이 조사포닌 함량을 높일 수 있음을 확인할 수 있었다.The results of measuring the irradiation phonin content of the thickened bellflower are shown in Table 6 above. As a result, compared to the comparative examples, the thickened bellflower of Preparation Example 1 exhibited a relatively high saponin content. Therefore, it was confirmed that the pre-treatment of bellflowers under the conditions of Preparation Example 1 to prepare thickened bellflowers can increase the content of irradiated ponins.

실시예 6. 관능검사Example 6. Sensory test

관능검사는 청소년 20명(남녀 10명씩), 성인 여성 30명, 성인 남성 30명, 65세 이상 일반인 20명(남녀 10명씩)하여 총 100명을 대상으로 제조예 1과 비교예들의 방법으로 제조한 증포 도라지를 차의 형태로 제조하여 섭취하게 하고 색, 향, 맛 및 전반적인 기호도를 구분하여 1점: 매우 나쁘다, 4점: 나쁘다, 3점: 보통이다, 4점: 좋다, 5점: 매우 좋음을 나타나는 5점 기호척도법으로 3 반복한 후 평균을 계산하여 나타내었다.The sensory test was prepared by the method of Preparation Example 1 and Comparative Examples for a total of 100 subjects with 20 adolescents (10 men and women), 30 adult women, 30 adult men, and 20 ordinary people 65 years or older (10 men and women each). Made in the form of tea, and made to consume one red bellflower, and classified color, scent, taste, and overall acceptability, 1 point: very bad, 4 points: bad, 3 points: moderate, 4 points: good, 5 points: very After 3 repetitions with a 5-point preference scale method indicating good, the average was calculated and shown.

증포 도라지의 관능검사Sensory test of the enlarged bellflower 시료sample color incense flavor 전반적인 기호도Overall acceptability 제조예 1Manufacturing Example 1 4.34.3 4.74.7 4.64.6 4.64.6 비교예 1Comparative Example 1 4.14.1 4.04.0 3.63.6 3.83.8 비교예 2Comparative Example 2 4.24.2 4.34.3 3.93.9 4.14.1 비교예 3Comparative Example 3 4.24.2 4.34.3 4.14.1 4.14.1 비교예 4Comparative Example 4 4.14.1 4.44.4 4.24.2 4.24.2

증포 도라지의 관능검사를 실시한 결과, 제조예 1의 증포 도라지가 향, 맛 및 종합 기호도에서 가장 높은 점수를 나타내어, 제조예 1의 증포 도라지가 소비자들의 기호에 가장 적합하면서 품질이 우수함을 확인할 수 있었다.As a result of conducting a sensory test of the enhanced bellflower, it was confirmed that the enhanced bellflower of Preparation Example 1 showed the highest score in aroma, taste, and overall preference, so that the enhanced bellflower of Preparation Example 1 was most suitable for the tastes of consumers and was excellent in quality. .

Claims (4)

(1) 물에 청주, 찹쌀 분말 및 결명자를 첨가하여 추출한 후 여과하여 침지액을 제조하는 단계;
(2) 도라지를 상기 (1)단계의 제조한 침지액에 침지하여 숙성시킨 후 꺼내어 세척하는 단계;
(3) 황칠, 칡, 치자, 로즈힙 및 생강을 혼합하여 약재 혼합물을 준비하는 단계;
(4) 상기 (3)단계의 준비한 약재 혼합물에 물을 첨가하여 추출한 후 여과하여 약용식물 추출액을 제조하는 단계;
(5) 상기 (4)단계의 제조한 약용식물 추출액을 찜기에 넣고 상기 (2)단계의 세척한 도라지를 찜기의 채반에 올려놓는 단계; 및
(6) 상기 (5)단계의 채반에 놓은 도라지에 상기 (4)단계의 제조한 약용식물 추출액을 분무하면서 찐 후 건조하는 단계를 포함하여 제조하는 것을 특징으로 하는 증포 도라지의 제조방법.
(1) adding and extracting Cheongju, glutinous rice powder, and Cassia tora to water and filtering to prepare an immersion solution;
(2) immersing the bellflower in the immersion liquid prepared in step (1), aging it, and taking it out and washing it;
(3) preparing a medicinal mixture by mixing hwangchil, arrowroot, gardenia, rosehip and ginger;
(4) preparing a medicinal plant extract by adding water to the medicinal mixture prepared in step (3), extracting it, and then filtering it;
(5) putting the medicinal plant extract prepared in step (4) in a steamer and placing the washed bellflower in step (2) on a tray of the steamer; And
(6) A method for producing a thickened bellflower, characterized in that it comprises the step of steaming and drying while spraying the extract of the medicinal plant prepared in the step (4) on the bellflower placed on the tray of the step (5).
제1항에 있어서, (3)단계의 약재 혼합물은 약재 혼합물 총 중량 기준으로, 황칠 58~62 중량%, 칡 18~22 중량%, 치자 8~12 중량%, 로즈힙 4~6 중량% 및 생강 4~6 중량%를 혼합한 약재 혼합물인 것을 특징으로 하는 증포 도라지의 제조방법.The method of claim 1, wherein the herbal mixture in step (3) is based on the total weight of the herbal mixture, 58 to 62% by weight of hwangchil, 18 to 22% by weight of arrowroot, 8 to 12% by weight of gardenia, 4 to 6% by weight of rosehip, and ginger. A method for producing a thickened bellflower, characterized in that it is a medicinal mixture in which 4 to 6% by weight is mixed. 제2항에 있어서,
상기 (1)단계는 물 18~22 L에 청주 45~55 g, 찹쌀 분말 45~55 g 및 결명자 45~55 g을 첨가하여 90~100℃에서 2~4시간 동안 추출한 후 여과하여 침지액을 제조하는 단계;
상기 (2)단계는 도라지를 상기 (1)단계의 제조한 침지액에 침지하여 25~35℃에서 5~7시간 동안 숙성시킨 후 꺼내어 세척하는 단계;
상기 (3)단계는 약재 혼합물 총 중량 기준으로, 황칠 58~62 중량%, 칡 18~22 중량%, 치자 8~12 중량%, 로즈힙 4~6 중량% 및 생강 4~6 중량%를 혼합하여 약재 혼합물을 준비하는 단계;
상기 (4)단계는 상기 (3)단계의 준비한 약재 혼합물에 약재 혼합물 대비 물 15~25배(v/w)를 첨가하여 90~100℃에서 2~4시간 동안 추출한 후 여과하여 약용식물 추출액을 제조하는 단계;
상기 (5)단계는 상기 (4)단계의 제조한 약용식물 추출액 18~22 L를 찜기에 넣고 상기 (2)단계의 세척한 도라지 18~22 ㎏을 찜기의 채반에 올려놓는 단계; 및
상기 (6)단계는 상기 (5)단계의 채반에 놓은 도라지에 상기 (4)단계의 제조한 약용식물 추출액을 분무하면서 110~130℃에서 증기로 20~40분 동안 찐 후 50~70℃에서 10~14시간 동안 건조하는, 상기 찌고 건조하는 과정을 8~10번 반복하는 단계를 포함하여 제조하는 것을 특징으로 하는 증포 도라지의 제조방법.
The method of claim 2,
In the step (1), 45-55 g of cheongju, 45-55 g of glutinous rice powder, and 45-55 g of Cassia tora are added to 18-22 L of water, extracted for 2-4 hours at 90-100°C, and filtered to obtain an immersion liquid. Manufacturing steps;
Step (2) is a step of immersing bellflower in the immersion liquid prepared in step (1), aging at 25 to 35°C for 5 to 7 hours, then taking out and washing;
The step (3) is based on the total weight of the herbal mixture, by mixing 58 to 62% by weight of hwangchil, 18 to 22% by weight of arrowroot, 8 to 12% by weight of gardenia, 4 to 6% by weight of rose hip, and 4 to 6% by weight of ginger. Preparing a medicinal mixture;
In step (4), 15 to 25 times (v/w) of water compared to the drug mixture is added to the drug mixture prepared in step (3), extracted for 2 to 4 hours at 90 to 100°C, and filtered to obtain a medicinal plant extract. Manufacturing steps;
The step (5) includes the steps of putting 18 to 22 L of the medicinal plant extract prepared in step (4) into a steamer and placing 18 to 22 kg of the washed bellflower in step (2) on a tray of the steamer; And
In step (6), while spraying the medicinal plant extract prepared in step (4) on the bellflower placed on the tray of step (5), steam at 110 to 130°C for 20 to 40 minutes with steam, and then at 50 to 70°C. Drying for 10 to 14 hours, the steaming and drying process is repeated 8 to 10 times, characterized in that the manufacturing method of the thickened bellflower.
제1항 내지 제3항 중 어느 한 항의 방법으로 제조된 증포 도라지.Increased bellflower produced by the method of any one of claims 1 to 3.
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Cited By (2)

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Publication number Priority date Publication date Assignee Title
KR102347048B1 (en) * 2021-06-01 2022-01-03 정재웅 Method for producing Kimchi using onion fermented solution
KR102367855B1 (en) * 2021-07-26 2022-02-25 농업회사법인 주식회사 유비무환 Method for manufacturing health supplement food to improve respiratory diseases

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KR100707345B1 (en) * 2006-01-20 2007-04-16 심중섭 Steamed bellflower
KR20190059628A (en) * 2017-11-23 2019-05-31 농업회사법인 주식회사 모이식품 Fermented balloon flower using detoxicating, fermenting and steaming process and manufacturing method thereof

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KR100707345B1 (en) * 2006-01-20 2007-04-16 심중섭 Steamed bellflower
KR20190059628A (en) * 2017-11-23 2019-05-31 농업회사법인 주식회사 모이식품 Fermented balloon flower using detoxicating, fermenting and steaming process and manufacturing method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102347048B1 (en) * 2021-06-01 2022-01-03 정재웅 Method for producing Kimchi using onion fermented solution
KR102367855B1 (en) * 2021-07-26 2022-02-25 농업회사법인 주식회사 유비무환 Method for manufacturing health supplement food to improve respiratory diseases

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