KR102239320B1 - Method for evaluating the objective masticatory function - Google Patents

Abstract

The present invention is a method of objectively and quantitatively evaluating the mastication function of a tooth using a masticatory function evaluation unit containing a fluorescent dye, specifically (a) authoring a masticatory function evaluation unit containing a fluorescent dye ; And (b) measuring the fluorescence intensity of the masticated unit using a quantitative light-induced fluorescence method (QLF).

Description

Method for evaluating the objective masticatory function

The present invention relates to a method capable of quantitatively evaluating the mastication function of a tooth.

Masticatory function refers to the ability to chew food using teeth, and a person chews food finely in the oral cavity through the mastication function to increase the area in contact with the digestive fluid, mix it well with saliva, and absorb it in the digestive tract. Do a series of actions to help you. After birth, people start training to eat solids little by little through the weaning machine and learn writing. Most of the organs in the oral cavity, such as teeth, tongue, lips, throat, throat, jaw joints, and salivary glands, are involved in these chewing behaviors, and learning is not done properly, or mastication dysfunction is caused by one or more dysfunctions of the organs. I can. When masticatory dysfunction occurs, food cannot be chewed properly, which can lead to various diseases such as inhibition of absorption of nutrients and digestive problems.

As described above, since the authoring function has very important significance for humans, it is a basic task in dental care to evaluate it objectively and accurately. Accordingly, methods for evaluating authoring functions are being developed using various methods. In particular, as a material for mastication function evaluation, the development of artificial food mass containing functional fine particles for mastication function evaluation (Japanese Patent Publication No. 2900947; Domestic Patent Application No. 10-2009-7002763) is actively being developed. However, since the method of evaluating the authoring function using these materials is subjective, the accuracy is inferior, or the processes of measuring the ratio for objective evaluation require expensive equipment, or complex experiments using organic solvents. It is not easy to use because it needs to be heard.

As described above, there is a need to develop an effective objective authoring function evaluation method that has high accuracy and sensitivity, short evaluation time, easy evaluation and understanding, and automation by objectively evaluating authoring functions.

An object of the present invention is to provide a method of objectively and quantitatively evaluating the mastication function of a tooth.

Another object of the present invention is to provide a method for determining masticatory dysfunction.

However, the technical problem to be achieved by the present invention is not limited to the problems mentioned above, and other problems that are not mentioned will be clearly understood by those of ordinary skill in the art from the following description.

Hereinafter, various embodiments described herein are described with reference to the drawings. In the following description, for a thorough understanding of the invention, various specific details, such as specific forms, compositions, and processes, are set forth. However, certain embodiments may be practiced without one or more of these specific details, or with other known methods and forms. In other instances, well-known processes and manufacturing techniques have not been described in specific details in order not to unnecessarily obscure the present invention. Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, form, composition, or characteristic described in connection with the embodiment is included in one or more embodiments of the present invention. Thus, the context of “in one embodiment” or “an embodiment” expressed in various places throughout this specification does not necessarily represent the same embodiment of the invention. Additionally, particular features, forms, compositions, or properties may be combined in one or more embodiments in any suitable manner.

In the present specification, "masticatory function" refers to a concept in a broad sense including the ability to chew food using teeth. Specifically, it refers to a series of actions that help digestion and absorption in the digestive tract by chewing food finely in the oral cavity to increase the area in contact with digestive juice, and to mix well with saliva. Chewing involves most organs in the oral cavity, such as teeth, tongue, lips, throat, throat, jaw joints, and salivary glands. It is called "disability". The disease may be dental caries, periodontal disease, hypersensitivity, tooth fracture, dry mouth, jaw facial muscle strength disorder, jaw joint disorder, facial nerve disorder, and tooth malocclusion, and the like, and is not limited thereto as long as it is related to the ability to chew food.

According to an embodiment of the present invention, (a) authoring a unit for evaluating a mastication function comprising a fluorescent dye; And

(b) It relates to a method of evaluating the mastication function of teeth, comprising the step of measuring the fluorescence intensity of the masticated unit by irradiating light.

In the present invention, the material of the unit for evaluating the mastication function is not particularly limited, but may be a material of wax or gum.

In addition, in the present invention, the shape of the unit for evaluating the mastication function is not particularly limited, and if a fluorescent dye is included, it may be included without limitation. For example, a fluorescent dye is filled inside a block-shaped gum base layer. ; A form in which a fluorescent dye is filled inside the tube-shaped gum base layer; A form in which a fluorescent dye is interposed between the two layers of the gum base layer; A form in which a gum base layer and a fluorescent dye are arranged in a lattice form; A polyhedral shape including a polyhedral fluorescent dye inside the polyhedral gum base layer; Alternatively, a fluorescent dye may be a core and a gum base layer may be in the form of a core/shell surrounding the core in a shell shape, but is not limited thereto.

1 to 6 show the shape of the unit for evaluating the mastication function according to an embodiment of the present invention, and may be a form in which a fluorescent dye 20 is filled inside the block-shaped gum base layer 10 as shown in FIG. 1. Alternatively, as shown in FIG. 2, the fluorescent dye 20 may be filled inside the tube-shaped gum base layer 10, and as shown in FIG. 3, the fluorescent dye 20 may be formed in a sheet shape between the two layers of gum base layer 10. The pigment 20 may be interposed, or the gum base layer 10 and the fluorescent dye 20 may be arranged in a grid as shown in FIG. 4, or a hexahedral gum as shown in the perspective view of FIG. 5. In the base layer 10, the fluorescent dye 20 is arranged as a complex polyhedron in which a plurality of hexahedrons are combined, or as shown in the cross-sectional structure of FIG. 6, the fluorescent dye 20 is a core and the gum base layer 10 is It may have a core/shell shape surrounding the core in a shell shape, but is not limited thereto.

In the present invention, the gum base layer includes a gum base, and may include sugar alcohol, sugars, emulsifiers, flavors, or other functional ingredients, but specific ingredients are not particularly limited.

In the present invention, the gum base is fiber (vegetable) such as methyl acetylricinolate, ester gum, vinyl acetate resin, polyisobutylene, polybutene, glycerin fatty acid ester, triacetin, calcium carbonate, calcium phosphate, powder pulp, etc. Inorganic materials such as talc (mineral), vegetable rubber-containing resins (gutta katiau, gutta hang kang, gutta percha, chicle, balata, venezuela chicle , Massaranduba chocolate, massaranduba balata, rosindinha, guayure, jelutong, sorva, sorbinha, tunu, Niger gutta, leche de vaca, rubber, Chilte, etc.), vegetable rubber-free resin (mastic, benzoin gum, Elemi resin) , Frankincense, Myrrh (Commiphora myrrha ENGL), Opopanax resin, Guaiac resin, Dammar resin, Kauri gum, Copal resin, Sandarak resin, Balsam Fir tree , Rosin, copaiba balsam, etc.), animal resin (shellac, etc.), vegetable wax (rice bran wax, sugarcane wax, urushi wax, wood wax, candelilla wax), Jojoba wax, ouricury wax, carnauba wax, oil seed wax, etc.), animal wax (sherak wax, spermaceti wax, beeswax, lanolin, etc.), mineral wax (ozokerite, paraffin wax, micro Crystalline wax, montan wax, etc.) may be used as raw materials, but is not limited thereto.

In the present invention, the fluorescent dye may be chlorophyll, flavonoids, or curcumin, but is not limited thereto.

In the present invention, the "chlorophyll" is a green pigment that can be extracted from autotropic and chemoautotrophic plants using an organic solvent. In general, 10 kinds of chlorophyll can be separated from the green part of various plants. The most abundant chlorophyll is chlorophyll a, followed by chlorophyll b, chlorophyll c1 and c2, chlorophyll d, protochlorophyll, bacteriochlorophyll and chlorobium chlorophyll. In the present invention, the chlorophyll is derived from green vegetables such as green beans, peas, wax beans, spinach, asparagus, broccoli, kale, parsley, parsley, leek, garland chrysanthemum, or sprout barley. However, it is not particularly limited.

In the present invention, the "flavonoid" is a ubiquitous natural polyphenol compound that often exhibits the bright and beautiful color of plants. It is concentrated in a number of fruits, plants, berries, grains, roots, stems, as well as beverages such as tea, coffee, beer and wine. Examples of the flavonoid compounds in the present invention include flavonols such as quercetin dihydrate or campferol; Flavones such as apigenin, luteolin, or diosmin; Flavanones such as naringenin or hesperidin (rhamnoglucoside of hesperitin); Catechins such as gallocatechin or epicatechin; Isoflavones such as genistein; Or anthocyanidin compounds such as peragonidine chloride; And the like, but is not limited thereto.

In the present invention, the unit for evaluating the mastication function may include a fluorescent dye and a gum base layer in a weight ratio of 1: 0.1 to 1: 10, but is not limited thereto.

In the present invention, the object of interest may perform the step of authoring the unit for evaluating the authoring function.

In the present invention, the "object of interest" refers to an individual whose chewing ability has decreased on one or both sides of the oral cavity, or is suspected to have decreased ability to decrease.

In the present invention, the number of times the object of interest authors the unit for evaluating the authoring function according to the present invention is not particularly limited, but, for example, 1 to 50 times, 1 to 30 times, 1 to 20 times, 1 time It may be performed 15 times, or 1 to 10 times, but is not limited thereto. However, as long as the authoring function can be judged, it is not limited thereto.

In the present invention, it may further include the step of classifying the authoring function evaluation unit authored after the authoring according to a classification method. At this time, the classification method is to classify the authored authoring function evaluation unit according to the number of authorings, and in more detail, 1 to 50 times, 1 to 30 times, 1 to 20 times, 1 to 15 times, Alternatively, a unit for evaluating the authoring function may be classified for each number of times from 1 to 10 times.

In the present invention, it is possible to perform the step of measuring the fluorescence intensity by visualizing the distribution of the fluorescent dye in the unit for evaluating the mastication function by irradiating light after the mastication.

In the present invention, the spectrum of the light or the light source is not particularly limited, and the fluorescence measurement wavelength may be changed according to the type of the fluorescent dye included in the unit for evaluating the authoring function, but, for example, excitation light having a wavelength of 300 to 550 nm ( light of excitation) can be investigated.

As an example of the present invention, when chlorophyll is used as the fluorescent dye, light having a wavelength of 440 to 460 nm may be irradiated, and preferably, when using chlorophyll a, light having a wavelength of 440 nm may be irradiated. , In the case of using chlorophyll b, light having a wavelength of 460 nm may be irradiated, but the present invention is not limited thereto.

As another example of the present invention, when flavonoids are used as the fluorescent dye, light having a wavelength of 520 nm may be irradiated, but the present invention is not limited thereto.

As another example of the present invention, when curcumin is used as the fluorescent dye, light having a wavelength of 368 to 404 nm may be irradiated, and preferably, excitation light having a wavelength of 368 nm or light having a wavelength of 404 nm may be irradiated, It is not limited thereto.

In the present invention, the light irradiation is preferably performed using quantitative light-induced fluorescence (QLF).

In the present invention, the fluorescence intensity can be measured by the following equation (1):

[Equation (1)]

Fluorescence intensity = (area of fluorescence area) / (area of total projection area) x 100

In Equation (1), the'area of the entire projection area' refers to the area of the plane of the unit that has been authored and transformed, and the'area of the fluorescent area' refers to the area where fluorescent colors are mixed in the unit by the authoring. Not limited.

According to another embodiment of the present invention, there is provided a method of determining a mastication function disorder by using the method of evaluating the mastication function of a tooth of the present invention.

In the present invention, the mastication dysfunction includes unilateral chewing because there is a disorder on one side of the oral cavity, or the mastication function is deteriorated on both sides of the oral cavity or as a whole.

In the present invention, after a certain number of mastication of the masticatory functional unit of the present invention to one side of the oral cavity of the object of interest, the same subject is selectively irradiated with light using quantitative light-induced fluorescence (QLF) to measure the fluorescence intensity of the masticated unit. After masticating the unit for the same shape to the other side of the oral cavity of the mouth the same number of times, measuring the fluorescence intensity of the chewed unit using quantitative light-induced fluorescence (QLF), and comparing it with the fluorescence intensity at one side of the oral cavity It may further include determining whether or not.

In the present invention, one side of the oral cavity may be the right or left side of the oral cavity, and the other side of the oral cavity may be the left or right side of the oral cavity, but is not particularly limited.

In the present invention, the mastication function is to measure the degree to which a fluorescent dye is incorporated when a unit is chewed at a certain number of times on one side of the oral cavity of a specific individual, and a fluorescent dye when the unit is chewed at the same number of times on the other side of the oral cavity of the same subject. It can be expressed by comparing the degree to which is incorporated.

In addition, in the present invention, after masticating the unit for the mastication function of the present invention in the object of interest, the fluorescence intensity of the masticated unit is measured by selectively irradiating light using quantitative light-induced fluorescence (QLF), and the same shape in the normal control group. The mastication function of the unit may be authored the same number of times, and then comparing the measured fluorescence intensity for the authored unit to determine a mastication function disorder of the object of interest.

In the present invention, the mastication function is to measure the degree to which a fluorescent dye is mixed when a specific individual chews the unit a certain number of times, and compare the degree of the fluorescent dye when a healthy primary malignant individual chews the unit the same number of times. It can be represented by

The authoring function evaluation method according to the present invention can quickly, accurately and objectively evaluate the authoring function. Moreover, the method of evaluating the mastication function of the present invention has significantly increased accuracy and sensitivity compared to the method of performing image analysis after mastication using a mastication function evaluator containing a colored pigment.

The mastication function evaluation method according to the present invention can be used to diagnose whether masticatory function is impaired, and through this, various oral diseases can be prevented, improved, or treated.

1 is a schematic diagram of the structure of a unit for evaluating authoring functions according to an embodiment of the present invention.
2 is a schematic diagram of the structure of a unit for evaluating authoring functions according to an embodiment of the present invention.
3 is a schematic diagram of the structure of a unit for evaluating authoring functions according to an embodiment of the present invention.
4 is a schematic diagram of the structure of a unit for evaluating authoring functions according to an embodiment of the present invention.
5 is a schematic diagram of the structure of a unit for evaluating authoring functions according to an embodiment of the present invention.
6 is a schematic diagram of the structure of a unit for evaluating authoring functions according to an embodiment of the present invention.
7 shows a photograph of the unit for evaluating the authoring function produced in Example 1.
8 shows a photograph of the unit for evaluating the authoring function produced in Example 2.
9 shows a photograph of the unit for evaluating the authoring function produced in Example 3.
10 shows a photograph of the unit for evaluating the authoring function produced in Example 4.
FIG. 11 shows an image photographed by quantitative light-induced fluorescence (QLF) after 1 to 10 mastication of the unit for evaluating the mastication function of Example 1 in Experimental Example 1. FIG.
12 shows an image photographed with quantitative light-induced fluorescence (QLF) after 1 to 10 mastication of the unit for evaluating the mastication function of Example 2 in Experimental Example 1. FIG.
13 shows an image photographed with quantitative light-induced fluorescence (QLF) after 1 to 10 mastication of the unit for evaluating the mastication function of Example 3 in Experimental Example 1. FIG.
14 shows an image photographed with quantitative light-induced fluorescence (QLF) after 1 to 10 mastication of the unit for evaluating the mastication function of Example 4 in Experimental Example 1. FIG.
15 is a graph showing the results of measuring the fluorescence intensity for each mastication number of the unit for evaluating the mastication function of Example 4 in Experimental Example 2;

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example

[Example 1] Preparation of a unit for evaluating authoring function

For the mastication function evaluation, a sheet-shaped gum base layer containing xylitol was prepared on the gum arabic base, and a fluorescent sheet layer using a spinach-derived chlorophyll fluorescent dye was interposed between the two gum base layers to obtain a unit for mastication evaluation. Was produced. The specific shape is shown in FIG. 7.

[Example 2] Preparation of a unit for evaluating authoring function

To evaluate the mastication function, a tube-shaped gum base layer containing xylitol was prepared in the gum base layer of gum, a through hole was formed in the center of the gum base layer, and then a chlorophyll fluorescent dye derived from spinach was filled to prepare a unit for mastication evaluation. I did. The specific shape is shown in FIG. 8.

[Example 3] Preparation of a unit for evaluating authoring function

For the evaluation of mastication function, a tube-shaped gum base layer containing xylitol was prepared in the gum base layer, a through hole was formed in the center of the gum base layer, and then a spinach-derived chlorophyll fluorescent dye was filled, and the upper and lower parts of the gum arabic gum The unit for mastication evaluation was produced by sealing with a base. The specific shape is shown in FIG. 9.

[Example 4] Fabrication of a unit for evaluating authoring function

For the mastication function evaluation, a tube-shaped gum base layer containing xylitol was prepared on the gum arabic base, and a unit for mastication evaluation was prepared in the same shape as in Example 3, but the amount of the fluorescent dye was half of that of Example 3. It was produced by filling only the capacity. The specific shape is shown in FIG. 10.

[Experimental Example 1] Evaluation of authoring function

In order to evaluate the mastication function, the subjects of the study were those who had all 28 natural teeth except wisdom teeth among men and women who were healthy whole body. Those with toothache, jaw joint disease, severe malocclusion, mastication disorder, severe dental caries, and fixed or removable dentures were excluded from the study. After distributing 10 units each prepared in Examples 1 to 4 to a total of 4 subjects selected in this way, each of them was chewed by the usual chewing method from 1 to 10 times. Then, the unit body chewed for each number of times was washed and dried in running water to prepare a unit according to the number of chewing times. Using the QLF-D Biluminator™2þ system (Inspector Research Systems BV, Amsterdam, The Netherlands), a fluorescence image of each unit was photographed by irradiating light with a wavelength of 440 nm, and the results are shown in FIGS. 11 to 14.

As shown in FIGS. 11 to 14, it was confirmed that the fluorescent dye was evenly mixed in the gum base layer as the number of mastications increased. In particular, when comparing FIGS. 13 and 14, in the case of FIG. 14 filled with only half of the fluorescent dye, FIG. 13 Compared to that, it was confirmed that the fluorescence brightness measured from the chewed unit was also decreased.

[Experimental Example 2] Evaluation of fluorescence intensity

In order to evaluate the fluorescence intensity, the total projection area (AH) and the fluorescence area (FA), which are essential variables for calculation, were calculated. Fluorescence intensity was measured according to Equation (1) below using these variables.

[Equation (1)]

Fluorescence intensity = (area of fluorescence area) / (area of total projection area) x 100

In Equation (1), the fluorescence intensity means the ratio of the area of the fluorescence area to the area of the entire projection area, and is set to a value between 0 and 100 in order to easily determine the authoring function from the result value.

As a result of the mastication evaluation using the unit body of Example 4 in Experimental Example 1, the total projection area (AH) and the fluorescence area (fluorescence area, FA) for each number of writings 1 to 10 times, and the corresponding Accordingly, the fluorescence intensity measured using Equation (1) is shown in Table 1 and FIG. 15 below.

Authoring count Area of the entire projection area [px] Area of fluorescence area [px] Fluorescence intensity (%) 0 97074 8248 8.496611 One 196739 23966 12.18162 2 103877 35858 34.51967 3 119847 48052 40.09445 4 61457 39821 64.7949 5 128257 98097 76.48471 6 111156 99056 89.1144 7 118075 113738 96.32691 8 79584 77678 97.60505 9 79256 78285 98.77486

As shown in Table 1 and FIG. 15, it was confirmed that the fluorescence intensity increased as the number of mastications increased.

Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and variations are possible without departing from the technical spirit of the present invention described in the claims. It will be obvious to those of ordinary skill in the field.

Claims (20)

Including the step of measuring the fluorescence intensity of the masticated unit by irradiating light to the unit for evaluation of the mastication function comprising a fluorescent dye authored in the object of interest,
The fluorescence intensity is measured by the following formula (1), a method for evaluating the mastication function of the tooth:
[Equation (1)]
Fluorescence intensity = (Area of fluorescence area) / (Area of total projection area) x 100
In Equation (1), the'total projection area' refers to the area of the plane of the unit whose shape has been converted by authoring, and the'fluorescent area' refers to the area where fluorescent colors are mixed in the unit by the work. The method of claim 1,
The mastication function evaluation unit is a material of wax or gum, a method for evaluating the mastication function of a tooth. The method of claim 1,
The unit for evaluating the mastication function is a form in which a fluorescent dye is filled inside a block-shaped gum base layer; A form in which a fluorescent dye is filled inside the tube-shaped gum base layer; A form in which a fluorescent dye is interposed between the two layers of the gum base layer; A form in which a gum base layer and a fluorescent dye are arranged in a lattice form; A polyhedral shape including a polyhedral fluorescent dye inside the polyhedral gum base layer; Or a core/shell form in which a fluorescent dye is a core and a gum base layer surrounds the core in a shell shape. The method of claim 1,
The fluorescent dye is chlorophyll, flavonoids, or curcumin, a method for evaluating the masticatory function of teeth. The method of claim 1,
The chewing is performed once to 50 times, the method for evaluating the chewing function of the teeth. The method of claim 1,
The light is a light having a wavelength of 300 to 550 nm, a method for evaluating the mastication function of a tooth. The method of claim 1,
The light irradiation is performed using quantitative light-induced fluorescence (QLF), a method for evaluating the masticatory function of a tooth. delete The method of claim 1,
The method for evaluating the mastication function of the tooth, characterized in that it further comprises the step of classifying the mastication function evaluation unit containing the fluorescent dye authored in the object according to the classification method. The method of claim 9,
The classification method is to classify the masticated function evaluation unit according to the number of mastication, the method for evaluating the mastication function of the tooth. Including the step of measuring the fluorescence intensity of the masticated unit by irradiating light to the unit for evaluation of the mastication function comprising a fluorescent dye authored in the object of interest,
The fluorescence intensity is measured by the following formula (1), a method of determining masticatory dysfunction:
[Equation (1)]
Fluorescence intensity = (Area of fluorescence area) / (Area of total projection area) x 100
In Equation (1), the'total projection area' refers to the area of the plane of the unit whose shape has been converted by authoring, and the'fluorescent area' refers to the area where fluorescent colors are mixed in the unit by the work. The method of claim 11,
The unit for evaluating mastication function is a material of wax or gum, a method of determining mastication function disorder. The method of claim 11,
The unit for evaluating the mastication function is a form in which a fluorescent dye is filled inside a block-shaped gum base layer; A form in which a fluorescent dye is filled inside the tube-shaped gum base layer; A form in which a fluorescent dye is interposed between the two layers of the gum base layer; A form in which a gum base layer and a fluorescent dye are arranged in a lattice form; A polyhedral shape including a polyhedral fluorescent dye inside the polyhedral gum base layer; Or a core/shell form in which a fluorescent dye is a core and a gum base layer surrounds the core in a shell shape. The method of claim 11,
The fluorescent dye is chlorophyll, flavonoids, or curcumin, a method for determining masticatory dysfunction. The method of claim 11,
The chewing is performed once to 50 times, the method of determining mastication dysfunction. The method of claim 11,
The chewing function evaluation unit is chewing on one side of the oral cavity of the object of interest, the method of determining mastication dysfunction. The method of claim 11,
The light is a light having a wavelength of 300 to 550 nm, a method of determining mastication dysfunction. The method of claim 11,
The light irradiation is performed using quantitative light-induced fluorescence (QLF). The method of claim 16,
Subsequent to the step of measuring the fluorescence intensity,
Measuring the fluorescence intensity of the masticated unit by irradiating light to the unit for evaluating the mastication function that has been masticated with respect to the other side of the oral cavity of the target object the same number of times; And
Comprising the step of comparing the fluorescence intensity of the chewed chewing function evaluation unit on one side of the oral cavity and the chewed chewing function evaluation unit on the other side of the oral cavity, further comprising the step of determining mastication dysfunction. The method of claim 16,
Comprising the step of comparing the fluorescence intensity measured in the step of measuring the fluorescence intensity with the fluorescence intensity measured for the masticatory function evaluation unit chewed after the same number of times in the normal control group, determining mastication dysfunction Way.

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