KR102231063B1 - Device of manufacturing for chip of diagnosing allergy - Google Patents

Abstract

Disclosed is a device for manufacturing a chip for diagnosing allergy. According to the present invention, provided is the device for manufacturing the chip for diagnosing allergy, comprising: an upper plate having a space in which an antigen-containing solution can be accommodated; and a suction unit provided under the upper plate and capable of moving the antigen-containing solution downward from the upper plate by the pressure difference between the outside and the inside by forming a negative pressure therein.

Description

Device of manufacturing for chip of diagnosing allergy

The present invention relates to an apparatus for manufacturing a chip for diagnosis of allergy.

Diagnostic chips for analyzing components in a sample are generally manufactured by coating an antigen or the like on a membrane-type fixture. Thereafter, various biological information can be obtained by reacting the antigen coated on the manufactured diagnostic chip with the sample to be analyzed.

Such diagnostic chips can be largely classified into a dot type and a line type. The dot-type diagnostic chip means a diagnostic chip in which an antigen, etc. is coated in a dot shape on a fixture, and the line-type diagnostic chip means a diagnostic chip in which an antigen or the like is coated in a line shape on a fixture. Among them, the dot-type diagnostic chip has the hassle of coating antigens and the like on the fixture in the form of dots one by one. On the other hand, the line-type diagnostic chip has an advantage in terms of productivity because it can be used in the form of a strip by coating an antigen or the like on a fixture in a line shape and then cutting it into a desired shape.

In such a line-type diagnostic chip, as the spacing between lines formed by coating increases, the number of lines to be coated relative to the same area increases, so that more various information can be obtained with only one detection. Therefore, in the case of a line-type diagnostic chip, it is necessary to achieve high integration to further maximize the number of coated lines compared to the same area.

Accordingly, the problem to be solved by the present invention is to manufacture a chip for allergy diagnosis that is highly integrated compared to the prior art.

According to an aspect of the present invention for achieving the above object, the upper plate is formed with a space in which a solution containing an antigen can be accommodated; And a solution containing the antigen from the upper plate due to a pressure difference between the outside and the inside by forming a negative pressure that is provided under the upper plate and having a pressure lower than the normal pressure therein. A suction unit that can be moved; Including, the upper plate, the upper surface portion formed with an injection hole through which the solution containing the antigen can be injected; And a lower surface provided under the upper surface and through which the solution containing the antigen can be discharged toward the lower plate. It is formed between the upper surface portion and the lower surface portion, connects the upper surface portion and the lower surface portion, and a plurality of thin plates are provided to be spaced apart from each other to form a plurality of inner spaces that are sealed to each other and accommodate the solution containing the antigen. Connection; And

A reinforcing plate connecting the plurality of thin plates formed in the connection portion; Including, on a lower surface of the upper plate, there is provided a chip manufacturing apparatus for allergy diagnosis in which a plurality of slits having a line shape are formed so that the antigen can be discharged to the outside in a line shape from the upper plate.

The connection portion may extend in a direction parallel to a direction in which the plurality of thin plates are arranged.

The reinforcing plate may be integrally formed with the plurality of thin plates.

The reinforcing plate and the plurality of thin plates are separate configurations, and the reinforcing plate and the plurality of thin plates may be coupled to each other.

A plurality of reinforcing plates may be provided, and the plurality of reinforcing plates may be provided to be spaced apart from each other in a width (Z) direction of the plurality of thin plates.

The width of the slit may be 0.2mm to 0.5mm.

Each of the plurality of inner spaces formed in the connection portion may communicate with one of the plurality of slits formed on the lower surface portion.

The thickness of the plurality of thin plates may be 0.5mm to 0.9mm.

On the upper surface of the upper plate, a plurality of slits having a line shape so that the antigen can be supplied to the upper plate are formed as the injection port, and each of the plurality of slits formed on the upper surface of the upper plate is formed at the connection part. It may communicate with one of the formed plurality of inner spaces.

In the upper surface of the upper plate, a plurality of supply holes having a dot shape are formed as the injection holes so that the antigen can be supplied to the upper plate, and the plurality of supply holes formed on the upper surface of the upper plate Each may communicate with one of a plurality of inner spaces formed in the connection part.

The number of the plurality of slits formed on the upper surface of the upper plate, the number of the plurality of slits formed on the lower surface of the upper plate, and the number of the plurality of inner spaces formed on the connection part of the upper plate may be the same. .

The number of the plurality of supply holes formed on the upper surface of the upper plate, the number of the plurality of slits formed on the lower surface of the upper plate, and the number of the plurality of internal spaces formed on the connection part of the upper plate may be the same. .

A lower plate provided under the upper plate and mounted on the suction unit; And a protective plate provided on an upper surface of the lower plate and configured to protect a membrane coated with the antigen. It may further include.

The protective plate may include a fibrous material.

The protective plate may include at least one or more from the group consisting of cellulose fibers, glass fibers, quartz fibers, and plastic fibers.

According to the present invention, it is possible to manufacture a chip for allergy diagnosis that is highly integrated compared to the prior art.

1 is a perspective view showing the structure of an apparatus for manufacturing a chip for diagnosing allergy according to the present invention.
2 is a side cross-sectional view showing the structure of an apparatus for manufacturing a chip for diagnosis of allergy according to the present invention.
3 is a plan view showing the structure of a suction unit in the apparatus for manufacturing a chip for diagnosis of allergy according to the present invention.
4 is a side cross-sectional view showing the structure of a suction unit in the apparatus for manufacturing a chip for diagnosis of allergy according to the present invention.
5 is a perspective view showing an example structure of an upper plate in the apparatus for manufacturing a chip for diagnosis of allergy according to the present invention.
6 is a plan view showing an example structure of an upper plate in an apparatus for manufacturing a chip for diagnosis of allergy according to the present invention.
7 is a cross-sectional view showing the structure of the upper plate cut along line AA of FIG. 6.
8 is a perspective view showing another example of the structure of an upper plate in the apparatus for manufacturing a chip for diagnosis of allergy according to the present invention.
9 is a plan view showing another example structure of an upper plate in the apparatus for manufacturing a chip for diagnosis of allergy according to the present invention.
10 is a cross-sectional view illustrating the structure of the upper plate cut along line BB of FIG. 9.
11 is a cross-sectional view showing the structure of the upper plate cut along line B'-B' of FIG. 9.
12 is a perspective view showing the structure of a lower plate in the apparatus for manufacturing a chip for diagnosis of allergy according to the present invention.
13 is a side cross-sectional view showing the structure of the lower plate cut along the line CC of FIG. 12.
14 is a plan view showing the structure of an allergy diagnosis chip according to the present invention.
15 is a view showing a coating state of a diagnostic chip manufactured according to an embodiment and a comparative example of the present invention.

Hereinafter, a structure of an apparatus for manufacturing a chip for diagnosis of allergy according to the present invention will be described with reference to the drawings.

Chip manufacturing device

1 is a perspective view showing the structure of an apparatus for manufacturing an allergy diagnosis chip according to the present invention, and FIG. 2 is a side cross-sectional view showing the structure of an apparatus for manufacturing an allergy diagnosis chip according to the present invention.

1 and 2, the device for manufacturing a chip for diagnosis of allergy according to the present invention 10 (hereinafter, referred to as a'manufacturing device') includes an upper plate 100 in which a solution containing an antigen can be accommodated. Can include. The upper plate 100 may be configured to receive a solution containing an antigen from the outside and discharge it downward.

A suction unit 200 capable of moving a solution containing an antigen contained in the upper plate 100 downward due to a pressure difference may be provided under the upper plate 100. The suction unit 200 according to the present invention moves the external air downward from the suction unit 200 by the difference between the pressure inside the suction unit and the pressure outside the suction unit, as will be described below, and accordingly, the inside of the upper plate 100 It may be of a configuration such that the solution containing the antigen of is discharged downward.

Meanwhile, referring to FIGS. 1 to 2, a lower plate 150 mounted on the suction unit 200 may be provided below the upper plate 100. The structures of the upper plate 100 and the lower plate 150 will be described later.

3 is a plan view showing the structure of a suction unit in the apparatus for manufacturing an allergy diagnosis chip according to the present invention, and FIG. 4 is a side cross-sectional view showing the structure of the suction unit in the apparatus for manufacturing an allergy diagnosis chip according to the present invention.

As shown in FIGS. 3 and 4, the suction unit 200 may have a space 210, which is a space with an open top, and the lower plate 150 to be mounted on the space 210. I can.

As described above, the solution containing the antigen contained in the upper plate 100 may be discharged downward by the suction unit. On the other hand, when the solution containing the antigen is discharged downward, a membrane having a thin thickness may be provided on the upper surface of the lower plate 150, and the solution discharged downward is sprayed onto the membrane so that the antigen is coated on the membrane. I can.

As shown in FIG. 4, the suction unit 200 may be provided with a flow path 220 that provides a path through which air is transported. The flow path 220 may be provided in plurality. 4 shows a case in which three flow paths 220 are provided.

In addition, the suction unit 200 may be connected to the flow path 220 and a negative pressure forming part (not shown) capable of forming a negative pressure therein may be formed. According to the present invention, when air existing inside the negative pressure forming unit is removed, the interior of the negative pressure forming unit is in a negative pressure state that is lower than the normal pressure. Accordingly, a pressure difference exists between the external pressure (ie, normal pressure) and the pressure inside the eup pressure forming unit (ie, negative pressure). At this time, when the flow path 220 of the suction unit 200 is opened, external air is supplied to the inside of the negative pressure forming unit due to the difference between the pressure inside the negative pressure forming unit and the external pressure, and at the same time, inside the upper plate 100. The received antigen is sprayed onto the membrane and coated.

Meanwhile, the negative pressure in the negative pressure forming unit may be formed by discharging the air in the negative pressure forming unit to the outside. Thereafter, air is introduced into the negative pressure forming unit while the solution containing the antigen is sprayed onto the membrane, and accordingly, the pressure in the negative pressure forming unit gradually increases. Then, when the pressure in the negative pressure forming part becomes the same as the normal pressure, the spraying of the solution toward the membrane may be terminated.

Meanwhile, the negative pressure forming part in the suction unit 200 may be connected to the outside for 7 seconds to 120 seconds by the flow path 220. If the negative pressure forming part is connected to the outside in less than 7 seconds, the solution containing the antigen may not be properly coated on the membrane in the form of a line during the process of coating the antigen on the membrane, and a problem may occur. Conversely, when the negative pressure forming part is connected to the outside for more than 120 seconds, too much time is spent in the process of coating the antigen on the membrane, resulting in a decrease in productivity. More preferably, the negative pressure forming part in the suction unit 200 may be connected to the outside for 7 seconds to 90 seconds by the flow path 220.

Before the flow path 220 is opened, a difference between the negative pressure and the normal pressure formed in the negative pressure forming portion of the suction unit 200 may be 1.8 bar to 2.2 bar. When the difference between the negative pressure and the atmospheric pressure in the negative pressure forming part is less than 1.8 bar, the rate at which the solution in the upper plate 100 is sprayed may not be sufficient during the antigen coating process of the membrane, so that the quality of the antigen coating may be deteriorated. On the other hand, if the difference between the negative pressure and the atmospheric pressure in the negative pressure forming part exceeds 2.2 bar, the rate at which the solution is sprayed during the antigen coating process of the membrane and the speed of the air flowing into the pressure forming part become too high, which may damage the membrane. have.

Referring back to FIGS. 1 and 2, the manufacturing apparatus 10 may further include a pressing unit 300 for pressing the upper plate 100 downward. The pressurization unit 300 according to the present invention lowers the upper plate 100 in the process of coating the antigen on the membrane and adheres to the lower plate 150 and pressurizes the antigen to the membrane provided on the upper surface of the lower plate 150. It may be a configuration that allows the coating to be made smoothly.

As shown in Fig. 2, the pressing unit 300 has a side pressing unit 310 that presses both sides of an upper portion of the upper plate 100 downward, and a central pressing unit that presses the center of the upper plate 100 downward. It may include (320). Between the side pressing portion 310 and the central pressing portion 320 may be driven independently of each other.

A stepped step 310a may be formed inside the side pressing part 310. In this case, the step 310a may have a shape corresponding to both sides of the upper plate 100. 2 shows a case where both sides of the upper plate 100 are formed vertically, and the step 310a facing both sides of the upper plate 100 is also formed vertically. When the step 310a has a shape corresponding to both sides of the upper plate 100, the side pressing part 310 not only serves to press the upper plate 100 downward, but also the upper plate 100 It may also serve to grip the upper plate 100 so as not to shake left and right.

FIG. 5 is a perspective view showing an example structure of an upper plate in an apparatus for manufacturing an allergy diagnosis chip according to the present invention, and FIG. 6 is a plan view showing an example structure of an upper plate in an apparatus for manufacturing an allergy diagnosis chip according to the present invention, 7 is a cross-sectional view showing the structure of the upper plate cut along line AA of FIG. 6.

As shown in FIGS. 5 to 7, according to an example, the upper plate 100 is provided under the upper surface 112 and the upper surface 112 in which the injection hole 112a into which the solution containing the antigen is injected is formed. It may include a lower surface portion 114 through which the solution containing the antigen is discharged toward the lower plate 150 (see FIG. 2). 5 to 7, according to an example of the upper plate according to the present invention, the injection hole 112a of the upper plate 100 may be a plurality of supply holes having a dot shape. Hereinafter, in the present specification, reference numeral 112a will be referred to as a'supply hole'.

Meanwhile, in an example of the upper plate 100 of the present invention, a plurality of slits 114a having a line shape may be formed on the lower surface portion 114. According to the present invention, a solution containing an antigen is discharged in a line shape through a plurality of slits 114a formed on the lower surface portion 114 of the upper plate 100, so that the antigen may be coated on the membrane in a line shape. That is, in this specification, the slit 114a does not mean a physical configuration, but means a space through which a solution containing an antigen is discharged.

The width of the plurality of slits 114a formed on the lower surface portion 114 of the upper plate 100 may be 0.2mm to 0.5mm. According to the present invention, since the width of the plurality of slits 114a formed on the lower surface portion 114 of the upper plate 100 is 0.2mm to 0.5mm, the membrane is coated when a solution containing an antigen is coated in a line shape. The thickness of the resulting line can also be set to 0.2mm to 0.5mm. Therefore, by reducing the thickness of the line coated on the membrane compared to the prior art, it is possible to achieve high integration of the diagnostic chip. More preferably, the width of the plurality of slits 114a formed on the lower surface portion 114 of the upper plate 100 may be 0.3mm to 0.5mm.

Continuing with reference to FIGS. 5 to 7, the upper plate 100 is formed on the upper surface portion 112 and the lower surface portion 114 and includes a connection portion 116 connecting the upper surface portion 112 and the lower surface portion 114. Can include. The connection portion 116 of the upper plate 100 may be understood as forming a body of the upper plate 100.

In this case, as shown in FIG. 7, a plurality of thin plates 116a may be provided inside the connection portion of the upper plate 100 to be spaced apart from each other. A plurality of inner spaces sealed to each other may be formed between the plurality of thin plates 116a, and a solution containing an antigen may be accommodated in the inner space between the plurality of thin plates 116a.

Meanwhile, the plurality of internal spaces formed by the plurality of thin plates 116a provided in the upper plate 100 may communicate with one of the plurality of slits 114a formed on the lower surface of the upper plate 100, respectively. . In addition, each of the plurality of supply holes 112a formed in the upper surface portion 112 of the upper plate 100 may communicate with one of the plurality of inner spaces formed in the connection portion 116 of the upper plate 100.

Therefore, a solution containing an antigen supplied through one of the supply holes 112a of the upper plate 100 is formed by a plurality of thin plates 116a and is in communication with the supply hole 112a. After flowing into the plurality of internal spaces, it may be discharged downward through the slit 114a communicating with the plurality of internal spaces.

The thickness of the plurality of thin plates 116a formed on the connection portion 116 of the upper plate 100 may be 0.5mm to 0.9mm. When the thickness of the thin plate 116a is less than 0.5mm, the thin plate 116a may be damaged by a rapid flow of fluid due to the operation of the suction unit 200 in the process of coating the antigen on the membrane. On the other hand, when the thickness of the thin plate 116a exceeds 0.9 mm, the width between lines coated on the membrane becomes too large, making it impossible to highly integrate the diagnostic chip.

The supply hole 112a of the upper surface portion formed in the upper plate 100, the inner space formed by the plurality of thin plates 116a, and the slit 114a of the lower surface portion may be in one-to-one communication with each other. Therefore, the number of supply holes 112a formed in the upper surface portion 112 of the upper plate 100, the number of the plurality of slits 114a formed in the lower surface portion 114 of the upper plate 100, and the upper plate The number of the plurality of inner spaces formed in the connection part 116 of 100 may be the same. In FIG. 7, some of the plurality of internal spaces formed in the connection portion 116 of the upper plate 100 are illustrated as not being in communication with the supply hole 112a of the upper surface portion 112, but as shown in FIG. Since the supply hole 112a may be formed over the entire area of the upper surface portion 112 of the upper plate, it should not be understood that the content shown in FIG. 7 does not correspond to the above content in this paragraph.

However, unlike this, the supply hole 112a of the upper surface portion formed in the upper plate 100 may correspond to the inner space formed by the plurality of thin plates 116a by n (n is an integer greater than or equal to 2) to 1. For example, the supply hole 112a of the upper surface formed in the upper plate 100 may correspond to the inner space formed by the plurality of thin plates 116a in a two-to-one manner.

FIG. 8 is a perspective view showing another example structure of an upper plate in an apparatus for manufacturing an allergy diagnosis chip according to the present invention, and FIG. 9 is a plan view showing another example structure of an upper plate in an apparatus for manufacturing an allergy diagnosis chip according to the present invention, 10 is a cross-sectional view showing the structure of the upper plate cut along line BB of FIG. 9 of FIG. 9. In addition, FIG. 11 is a cross-sectional view showing the structure of the upper plate cut along line B'-B' of FIG. 9. Hereinafter, in the description of another example of the upper plate, it overlaps with a description of an example of the upper plate. The part that is to be omitted will be described focusing on contents different from one example of the upper plate.

8 to 9, according to another example, a slit 112b having a line shape so that a solution containing an antigen can be supplied to the upper plate 112 on the upper surface 112 of the upper plate 100. This injection hole may be formed in plural, and each of the plurality of slits 112b formed on the upper surface part 112 of the upper plate 100 is among a plurality of internal spaces formed in the connection part 116 of the upper plate 100. You can communicate with one.

Therefore, the solution containing the antigen supplied through one of the slits 112b of the upper surface portion 112 of the upper plate 100 is formed by a plurality of thin plates 116a and communicated with the slit 112b. After flowing into the plurality of inner spaces, it may be discharged downward through the slit 114a of the lower surface portion 114 communicating with the plurality of inner spaces.

In addition, the upper slit 112b formed on the upper plate 100, the inner space formed by the plurality of thin plates 116a, and the lower slit 114a may be in one-to-one communication with each other. Therefore, the number of the plurality of slits 112b formed in the upper surface portion 112 of the upper plate 100, the number of the plurality of slits 114a formed in the lower surface portion 114 of the upper plate 100, and the upper plate ( The number of the plurality of inner spaces formed in the connection part 116 of 100) may be the same. In FIG. 10, some of the plurality of inner spaces formed in the connection portion 116 of the upper plate 100 are shown as not being in communication with the slit 112b of the upper surface portion 112, but as shown in FIG. Since (112b) is formed over the entire area of the upper surface portion 112 of the upper plate, it should not be understood that the content shown in Fig. 9 does not correspond to the above content of this paragraph.

Meanwhile, the upper plate 100 may have a shape formed by mixing the supply hole 112a and the slit 112b described above. That is, the upper plate 100 according to the present invention may have a structure in which both the supply hole 112a and the slit 112b are formed.

On the other hand, as shown in Figs. 8, 9 and 11, according to the present invention, the upper plate 100 further includes a reinforcing plate 120 connecting a plurality of thin plates 116a (see Fig. 10) formed in the connection part. can do. The reinforcing plate 120 may extend in a direction parallel to a direction in which a plurality of thin plates are arranged.

As described above, according to the present invention, the thickness of the plurality of thin plates formed at the connection portion of the upper plate is 0.5mm to 0.9mm, which is quite thin. Therefore, in the process of forming a negative pressure by the suction unit and moving the solution containing the antigen downward through the upper plate, an impact due to the flow of fluid may be applied to the plurality of thin plates having a thin thickness, and in severe cases, such an impact Some of the thin plate may be damaged or torn due to vibration caused by.

The reinforcing plate 120 shown in FIGS. 8, 9, and 11 may be configured to improve durability of a plurality of thin plates. That is, according to the present invention, the reinforcing plate 120 on the upper plate absorbs the impact applied to the plurality of thin plates and can suppress the movement of the plurality of thin plates. It is possible to solve the problem of damage or tearing of a plurality of thin plates.

According to the present invention, only one reinforcing plate 120 may be provided, but a plurality of reinforcing plates 120 may be provided. When a plurality of reinforcing plates 120 are provided, the plurality of reinforcing plates may be provided to be spaced apart from each other in the width (Z) direction of the plurality of thin plates. In FIGS. 8, 9 and 11, as an example of a case in which a plurality of reinforcing plates 120 are provided, three reinforcing plates 120 are provided to be spaced apart from each other at equal intervals in the width (Z) direction of the plurality of thin plates. It is shown that the plurality of thin plates are divided into four regions each having the same width by the reinforcing plate 120.

On the other hand, in an example of the present invention, the reinforcing plate 120 of the upper plate 100 may be formed integrally with a plurality of thin plates 116a. At this time, the fact that the reinforcing plate and the plurality of thin plates are integrally formed means that the reinforcing plate and the plurality of thin plates are in an inseparable bonding relationship without a separate bonding or assembling process. It could mean that the two configurations cannot be separated from each other.

Alternatively, in another example of the present invention, the reinforcing plate 120 and the plurality of thin plates 116a of the upper plate 100 may have separate configurations. Accordingly, in another example of the present invention, the reinforcing plate 120 and the plurality of thin plates 116a may be coupled to each other. For example, the plurality of thin plates 116a may be fitted into a groove (not shown) formed in the reinforcing plate 120.

As described above, the reinforcing plate 120 may be a configuration for improving the durability of the plurality of thin plates 116a constituting the upper plate 100. On the other hand, the fluidity of the solution containing the antigen in the upper plate 100 may be degraded by the reinforcing plate 120. In particular, such a decrease in fluidity may occur significantly at the upper end of the plurality of thin plates 116a into which the solution containing the antigen is introduced and the lower end of the plurality of thin plates 116a through which the solution containing the antigen is discharged. To prevent this, the upper end of the reinforcing plate 120 may be formed below the upper end of the plurality of thin plates 116a, and the lower end of the reinforcing plate 120 is less than the lower end of the plurality of thin plates 116a. It can be formed on the top. In this case, when the solution containing the antigen is introduced into the upper plate 100 and when the solution is discharged from the upper plate 100, the influence of the solution by the reinforcing plate 120 may be minimized.

12 is a perspective view showing the structure of a lower plate in the apparatus for manufacturing a chip for diagnosis of allergy according to the present invention, and FIG. 13 is a side cross-sectional view showing the structure of the lower plate cut along line C-C of FIG. 12.

As shown in FIGS. 12 and 13, the lower plate 150 may include an upper surface portion 162 in which a plurality of slits 162a are formed and a lower surface portion 164 in which a plurality of slits 164a are formed. . Similar to the case of the upper plate, the slits 162a and 164a formed on the lower plate 150 also mean a physical space.

The slits 162a and 164a formed on the upper and lower surfaces 162 and 164 of the lower plate 150, respectively, allow external air to flow into the suction unit while the solution containing the antigen is coated on the membrane. You can provide a path. That is, as described above, when negative pressure is formed in the suction unit 200 during the coating process of the membrane, external air is transferred to the slit 162a and the lower surface 164 formed on the upper surface 162 of the lower plate 150. After passing through the slit (164a) formed in the sequence may be supplied to the suction unit. On the other hand, similar to the case of the upper plate, a connection portion connecting the upper surface portion 162 and the lower surface portion 164 may be formed in the lower plate 150, and a plurality of thin plates spaced apart from each other may be formed inside the connection portion. have. Each of the inner spaces between the plurality of thin plates may communicate with one of the slits 162a of the upper surface portion 162 and one of the slits 164a of the lower surface portion 164.

Meanwhile, the apparatus for manufacturing a chip for diagnosis of allergy according to the present invention may further include a protective plate (not shown) provided on the upper surface of the lower plate 150.

The protective plate may be configured to protect the membrane. As described above, according to the present invention, when the flow path 220 (refer to FIG. 4) of the suction unit 200 (refer to FIG. 2) is opened, external air is generated by the difference between the pressure inside the negative pressure forming part and the pressure outside. Is supplied to the inside of the negative pressure forming part, and at the same time, the antigen contained in the upper plate 100 is sprayed onto the membrane, so that the antigen is coated on the membrane.

In such a coating process, since a fluid including air flows around or through the membrane at a high velocity, the membrane may be damaged or torn during the coating process of the antigen. In particular, when the membrane is directly provided on the upper surface of the lower plate, problems such as the membrane being sucked into the slit formed on the upper surface of the lower plate during the coating process may occur.

The protective plate may be a configuration for solving the above problem that the membrane is damaged or torn during the coating process of the antigen. That is, according to the present invention, since the protective plate is provided on the upper surface of the lower plate 150 and the membrane is provided on the upper surface of the protective plate, the protective plate is provided between the membrane and the lower plate 150. Therefore, it is possible to prevent the membrane from being sucked into the slit 162a formed on the upper surface portion 162 of the lower plate 150, as well as minimize the impact of the fluid flowing into the lower plate 150 to the membrane. can do.

On the other hand, in the process of coating the membrane with the antigen, if the air that has passed through the membrane is not properly discharged through the lower plate, the solution containing the antigen will not flow smoothly, so the coating of the antigen on the membrane is also not performed smoothly. I can't. However, as described above, according to the present invention, since the protective plate may be provided between the protective plate and the lower plate, the protective plate needs to be made of a material having air permeability in order to properly discharge the air passing through the membrane through the lower plate. .

Accordingly, according to the present invention, the protective plate may include a fibrous material having air permeability. Alternatively, the protective plate may be made of a fibrous material having air permeability.

In more detail, the protective plate may include at least one fiber material from the group consisting of cellulose fiber, glass fiber, quartz fiber, and plastic fiber, or may be made of at least one fiber material. The cellulosic fibers may be alpha cellulose fibers, and the glass fibers may be borosilicate glass fibers. In addition, the quartz fiber may be a silica fiber, and the plastic fiber may be a PTFE (Polytetrafluoroethylene) fiber.

Since the glass fiber has high heat resistance and corrosion resistance, it is possible to minimize deterioration during the coating process on the membrane, and high air permeability, thereby increasing the coating efficiency. In addition, the quartz fiber has high heat resistance and the plastic fiber has high corrosion resistance, so it is possible to minimize deterioration during the coating process on the membrane like glass fiber.

Meanwhile, in the upper plate according to the present invention, a ratio of the width of the slit to the thickness of the thin plate may be 0.4 to 0.55. More preferably, it may be 0.40 to 0.55.

14 is a plan view showing the structure of an allergy diagnosis chip according to the present invention.

As shown in FIG. 14, the allergy diagnosis chip 400 according to the present invention may be manufactured by the apparatus 10 for manufacturing an allergy diagnosis chip according to the present invention.

The allergy diagnosis chip 400 may include a sheet-shaped membrane 400a and a coating portion 410 that is an antigen-coated region on the membrane 400a. A plurality of coating units 410 may be formed, and may have a strip shape having a predetermined thickness.

The allergy diagnosis chip 400 may have a rectangular shape having a constant width (W) and length (L), and a plurality of coating portions 410 having a constant thickness (t) may have a width ( It may be provided to be spaced apart from each other by a certain distance (d) along the direction W).

Since the allergy diagnosis chip 400 according to the present invention can form a large number of coatings compared to the same area of the chip compared to the prior art, the allergy diagnosis chip 400 may be highly integrated. To this end, in the allergy diagnosis chip 400 according to the present invention, there may be a constant ratio between the distance d between the coating portions 410 and the thickness t of the coating portions 410.

That is, in the allergy diagnosis chip 400 according to the present invention, the ratio of the thickness (t) of the coating part 410 to the distance (d) between the coating parts 410 (that is, t/d) may be 0.40 to 0.55. have.

In addition, according to the present invention, the thickness t of the coating part 410 may be 0.2mm to 0.5mm. In addition, the distance (d) between the coating portions 410 may be 0.5mm to 0.9mm.

Example 1

After fixing the lower plate on the upper part of the space of the suction unit through fastening, a membrane was provided on the upper surface of the lower plate. In addition, after gripping both sides of the upper plate with the side pressing part, the side pressing part and the central pressing part were moved downward to bring the upper plate into close contact with the lower plate and the membrane. In addition, air in the negative pressure forming part of the suction unit was discharged to create a negative pressure state lower than normal pressure by 2 bar.

Thereafter, by opening the flow path of the suction unit, external air was introduced into the negative pressure forming part, and a solution containing an antigen was coated on the membrane to prepare a diagnostic chip. The flow path of the suction unit was opened for 7 seconds. As air was introduced into the negative pressure forming part, the pressure in the negative pressure forming part gradually increased, and after the coating was completed, the pressure in the negative pressure forming part reached normal pressure.

Example 2

The membrane was coated with an antigen-containing solution in the same manner as in Example 1, except that the flow path of the suction unit was opened for 30 seconds.

Example 3

The membrane was coated with an antigen-containing solution in the same manner as in Example 1, except that the flow path of the suction unit was opened for 60 seconds.

Example 4

The membrane was coated with an antigen-containing solution in the same manner as in Example 1, except that the flow path of the suction unit was opened for 120 seconds.

Comparative example

The membrane was coated with an antigen-containing solution in the same manner as in Examples, except that the flow path of the suction unit was opened for 5 seconds.

Experimental Example 1

The antigen-coated state of the diagnostic chips prepared according to Examples and Comparative Examples was observed. The observation results are shown in FIG. 15. 15A is a diagram illustrating a coating state of a diagnostic chip according to a comparative example, and FIGS. 15B to 15E illustrate a coating state of a diagnostic chip according to Examples 1 to 4, respectively. will be.

As shown in (a) of FIG. 15, in the case of the comparative example, it was confirmed that a part of the solution containing the antigen was smeared around the line coated on the diagnostic chip. That is, in the case of the comparative example, it can be confirmed that the coating was not properly formed on the membrane because the time for the formation of the negative pressure was not sufficiently secured.

As shown in (b) to (e) of Figure 15, in the case of Examples 1 to 4, it was confirmed that only the line coated on the diagnostic chip was coated normally.

Experimental Example 2

In the diagnostic chips prepared according to Examples 1 to 4 and Comparative Examples, the thickness (t) of the coating layer coated with the solution containing the antigen and the distance (d) between the coating layers were measured.

In the diagnostic chips manufactured according to Examples 1 to 4, the thickness (t) of the coating layer was measured to be 0.4 mm, respectively, and the spacing (d) between the coating layers was measured to be 0.75 mm. Therefore, the ratio (t/d) of the thickness (t) of the coating portion to the distance (d) between the coating portions was measured to be 0.533.

On the other hand, in the case of the comparative example, since the solution was not uniformly coated and part of the solution spread around the coated line, the thickness of the coating layer and the distance between the coating layers could not be measured.

In the above, although the present invention has been described by limited embodiments and drawings, the present invention is not limited thereto, and the technical idea of the present invention and the following description by those of ordinary skill in the art to which the present invention pertains. It goes without saying that various implementations are possible within the equal range of the claims to be made.

10: manufacturing device
100: upper plate
112: upper surface
112a: supply hole
112b: slit
114: lower surface
114a: slit
116: connection
116a: thin plate
120; Reinforcement plate
150: lower plate
162: upper surface
162a: slit
164: lower surface
164a: slit
200: suction unit
210: space part
220: Euro
300: pressurization unit
310: side pressing part
310a: step
320: central pressurization part
400: Allergy diagnosis chip
400a: membrane
410: coating part

Claims (15)

An upper plate having a space in which a solution containing an antigen can be accommodated; And
The solution containing the antigen is moved downward from the upper plate due to a pressure difference between the outside and the inside by forming a negative pressure that is provided under the upper plate and having a pressure lower than the normal pressure inside. A suction unit that can be made; Including,
The upper plate,
An upper surface portion in which an injection hole through which the solution containing the antigen can be injected is formed;
A lower surface provided under the upper surface and through which the solution containing the antigen can be discharged toward the lower plate;
It is formed between the upper surface portion and the lower surface portion, connects the upper surface portion and the lower surface portion, and a plurality of thin plates are provided to be spaced apart from each other to form a plurality of inner spaces that are sealed to each other and accommodate the solution containing the antigen. Connection; And
A reinforcing plate connecting the plurality of thin plates formed in the connection portion; Including,
On the lower surface of the upper plate,
A chip manufacturing apparatus for allergy diagnosis in which a plurality of slits having a line shape are formed so that the antigen can be discharged to the outside in a line shape from the upper plate. In claim 1,
The device for manufacturing an allergy diagnosis chip extending in a direction parallel to a direction in which the plurality of thin plates are arranged. In claim 1,
The reinforcing plate is an allergy diagnosis chip manufacturing apparatus that is integrally formed with the plurality of thin plates. In claim 1,
The reinforcing plate and the plurality of thin plates are separate configurations,
Allergy diagnosis chip manufacturing apparatus in which the reinforcing plate and the plurality of thin plates are coupled to each other. In claim 1,
The reinforcing plate is provided in plurality,
The plurality of reinforcing plates are provided to be spaced apart from each other in a width (Z) direction of the plurality of thin plates. In claim 1,
The width of the slit is 0.2mm to 0.5mm allergy diagnosis chip manufacturing apparatus. In claim 1,
The apparatus for manufacturing an allergy diagnosis chip in which the plurality of internal spaces formed in the connection part communicate with one of the plurality of slits formed in the lower surface part. In claim 7,
The thickness of the plurality of thin plates is 0.5mm to 0.9mm allergy diagnosis chip manufacturing apparatus. In claim 7,
A plurality of slits having a line shape so that the antigen can be supplied to the upper plate are formed on the upper surface of the upper plate as the injection port,
Each of the plurality of slits formed on the upper surface of the upper plate communicates with one of the plurality of internal spaces formed on the connection part. In claim 7,
In the upper surface of the upper plate, a plurality of supply holes having a dot shape are formed as the injection ports so that the antigen can be supplied to the upper plate,
Each of the plurality of supply holes formed on the upper surface of the upper plate communicates with one of a plurality of internal spaces formed on the connection part. In claim 9,
Allergy diagnosis chips in which the number of the plurality of slits formed on the upper surface of the upper plate, the number of the plurality of slits formed on the lower surface of the upper plate, and the number of the plurality of internal spaces formed on the connection part of the upper plate are the same Manufacturing device. In claim 10,
Allergy diagnosis chips having the same number of supply holes formed on the upper surface of the upper plate, the plurality of slits formed on the lower surface of the upper plate, and the number of internal spaces formed at the connection part of the upper plate Manufacturing device. In claim 1,
A lower plate provided under the upper plate and mounted on the suction unit; And
A protective plate provided on an upper surface of the lower plate and configured to protect a membrane coated with the antigen; Allergy diagnosis chip manufacturing apparatus further comprising a. In claim 13,
The protective plate is a chip manufacturing apparatus for allergy diagnosis comprising a fiber material. In claim 14,
The protective plate is a chip manufacturing apparatus for allergy diagnosis comprising at least one or more from the group consisting of cellulose fiber, glass fiber, quartz fiber, and plastic fiber.

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