KR102203808B1 - Method of producing extract having enhanced contents of gensenoside Rg6, Rk3 and Rh4 from Panax ginseng leaf and use of the extract - Google Patents

Abstract

It provides a method for efficiently producing a ginseng leaf extract having an increased content of rare ginsenosides Rg6, Rk3 and Rh4, and a use of the ginseng leaf extract produced thereby.

Description

Method of producing extract having enhanced contents of gensenoside Rg6, Rk3 and Rh4 from Panax ginseng leaf and use of the extract}

The present invention relates to a ginseng leaf extract with increased ginsenosides Rg6, Rk3 and Rh4 through microwave processing, and a production method thereof.

Ginseng ( Panax ginseng ) is a perennial herbaceous plant that grows wild in the mountains of northeast Asia, and is classified as genus Panax of the Araliaceae or Ogalpidae. In the genus Panax, American ginseng ( Panax quinquefolia ), Panax trifolia ( Panax trifolia ), Panax notoginseng ( Panax notoginseng ), Himalayan ginseng ( Panax pseudoginseng ), etc. are known in North America. They show distinct differences in the shape of roots, stems, and leaves, and there are also large differences in component composition and content by site ( J Ginseng Res , 39 , 287-298, 2014. ).

Until now, studies on the roots of ginseng have been mainly concentrated. However, studies on stems, leaves, and fruits excluding the roots have been insufficient. In particular, ginseng leaves have not been proven to be effective in terms of economics. In most cases, the leaves were discarded.

Ginsenoside, the main component of ginseng's efficacy, is called ginseng saponin, mainly in the form of triterpene glycosides, which consist of dammarane-based non-glycosides and several different saccharides. Female species are known, and different ginsenosides contained in ginseng are reported to have various medicinal effects.

Ginsenosides Rg6, Rk3 and Rh4 are compounds represented by formulas 1, 2, and 3, respectively. Rk3 and Rh4 are in an isomeric relationship with each other.

(식 1)

(Equation 1)

(식 2)

(Equation 2)

(식 3)

(Equation 3)

There is a need for a method of efficiently producing a ginseng extract having an increased content of rare ginsenosides Rg6, Rk3 and Rh4, and the use of the ginseng extract produced thereby.

One aspect is that of a plant extract of the genus Panax containing 20% or more by weight of rare ginsenosides Rg6, Rk3 and Rh4 with respect to the total weight of ginsenosides comprising the step of microwave irradiating the leaves of the genus Panax or the extract thereof. As a production method, the total ginsenosides are Rg1, Re, Rh1, Rg2, Rb1, Rc, Rb2, Rd, Rg6, Rk3, Rh4, F2, S -Rg3, R -Rg3, Rk1, CY, CK, Rk1, Rg5, Rh2, Rrk2, and Rh3.

Another aspect provides an extract of the genus Panax plant prepared by the above method.

Another aspect provides a pharmaceutical composition comprising an extract of the genus Panax plant prepared by the above method.

Another aspect provides a food composition comprising a plant extract of the genus Panax prepared by the above method.

Another aspect provides a cosmetic composition for improving skin wrinkles comprising a plant extract of the genus Panax prepared by the above method.

One aspect of the plant extract of the genus Panax containing 20% or more by weight of rare ginsenosides Rg6, Rk3 and Rh4 with respect to the total weight of ginsenosides comprising the step of microwave irradiation of the leaves of the genus Panax plant or extracts thereof. As a production method, the total ginsenosides are Rg1, Re, Rh1, Rg2, Rb1, Rc, Rb2, Rd, Rg6, Rk3, Rh4, S -Rg3, R -Rg3, Rk1, and Rg5. do.

In the above method, the weight of the rare ginsenoside is 20% or more, 25% or more, 30% or more, 35% or more, 40% or more, 45% or more, 50% or more, 55% with respect to the total ginsenoside. Or more, 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, 85% or more, 90% or more, 20 to 95%, 30 to 95%, 40 to 95%, 50 to 95%, It may be 60 to 95%, 70 to 95%, or 70 to 95%.

The plant extract of the genus Panax obtained by the above method has a weight of Rg6 of 4.5% by weight or more, for example, 4.5 to 20%, 4.5 to 16%, 5.0 to 20%, 5.0 to 16, based on the total ginsenoside weight. %, 6.0 to 16%, 7.0 to 16%, 8.0 to 16%, 9.0 to 16%, or 10.0 to 16%, the weight of Rk3 relative to the total weight of ginsenoside is 2.0% by weight or more, for example, 2.0 To 32.0%, 5.0 to 32.0%, 8.0 to 32.0%, 11.0 to 32.0%, 13.0 to 32.0%, or 14.0 to 32.0%, the weight of Rh4 relative to the total weight of ginsenoside is 2.5% by weight or more, for example , 2.5 to 47.5%, 5.0 to 47.5%, 7.5 to 47.5%, 10.0 to 47.5%, 14.0 to 47.5%, 20.0 to 47.5%, 30.0 to 47.5%, or 40.0 to 47.5%, or a combination thereof I can. The obtained panax plant extract may include Rg6: Rk3: Rh4 in a content ratio of 5 to 16: 7 to 32: 8.0 to 48. The plant extract of the genus Panax obtained by the above method may be extracted with a solvent and may not be fractionated.

The plant of the genus Panax is Korean ginseng ( Panax ginseng ), Hwagi ginseng ( Panax quinquefolia ), Jeonchil ginseng ( Panax notoginseng ), Jukjeolsam ( Panax japonica ), Trifolia ( Panax trifolia ), Himalayan ginseng ( Panax pseudoginseng ), Vietnamese ginseng ( Panax vietnamensis ), or a combination thereof. The leaves may be at any time during growth. For example, the leaves may be 30 to 120 days after growth. The leaf may be a leaf from a germination leaf of ginseng to a leaf before ginseng harvest.

The ginseng leaf may include ginsenoside Rg1, and Re. The ginseng leaf may include, for example, Rg1, and Re, Rb1, Rc, Rb2 and Rd.

The microwave irradiation may be performed in a liquid medium containing the leaves of the genus Panax or extracts thereof. The liquid medium may be water, C1-C6 alcohol or mixtures thereof. The C1-C6 alcohol can be methanol, ethanol, propanol, butanol, pentanol, or hexanol. The mixture may be a mixture of water and methanol, ethanol, propanol, butanol, pentanol, or hexanol. The mixture may be a mixture of 30 to 100%, 30 to 80%, 30 to 60%, or 40 to 60%, or 45 to 55% methanol, ethanol, propanol, butanol, pentanol, or hexanol in water. .

In the above method, in the "leaf of the genus Panax or an extract thereof", the "extract" may include at least 55% by weight of Rg1 and Re based on the total ginsenoside. "The extract" may be one containing 55% by weight or more of Rg1 and Re of the total ginsenosides by weight. The "extract" may be an extract of water from the leaves of the genus Panax, C1-C6 alcohol, or mixtures thereof. "The extract" may be extracted by contacting the leaves of the genus Panax with a solvent. "The extract" may be extracted by a method comprising contacting with water, C1-C6 alcohol, or a mixture thereof at 50° C. to reflux temperature.

In the above method, the microwave irradiation refers to a thermal reaction in which a ginseng leaf extract is irradiated with microwaves and heated. The microwave may be a micropile having a frequency of 300 MHz to 300 GHz, for example, 1000 MHz to 100 GHz, 1000 MHz to 50 GHz, 1000 MHz to 10 GHz, or 1000 MHz to 5 GHz.

The microwave irradiation conditions are not particularly limited, and may include any reaction conditions such that 20% or more of Rg6, Rk3, and Rh4 are contained with respect to the total ginsenoside weight by microwave irradiation. The product of the microwave irradiation may be used as it is, but may be contained as a dried product or a freeze-dried product.

The microwave irradiation may be performed at pH 5.0 to 7.0, pH 5.0 to 6.0, or pH 6.0 to 7.0. Microwave irradiation may be irradiated in a neutral solution, for example, an aqueous solution. The aqueous solution may be water, a C1-C6 alcohol, or a mixture thereof. The C1-C6 alcohol can be methanol, ethanol, propanol, butanol, pentanol, or hexanol. The mixture may be a mixture of water and methanol, ethanol, propanol, butanol, pentanol, or hexanol. The mixture may be a mixture of 30 to 100%, 30 to 80%, 30 to 60%, or 40 to 60%, or 45 to 55% methanol, ethanol, propanol, butanol, pentanol, or hexanol in water. . The output of the microwave is not particularly limited, and may be appropriately increased or decreased according to the amount of the reactant, and for example, a microwave of 50 to 1000 W, for example, 100 to 700 W may be used.

The microwave irradiation is under pressure, for example, 1 to 100 atm, 2 to 100 atm, 5 to 100 atm, 7 to 100 atm, 10 to 100 atm, 15 to 100 atm, 1.5 atm To 80 atm, 2 to 80 atm, 5 to 80 atm, 7 to 80 atm, 10 to 80 atm, 15 to 80 atm, 1.5 to 50 atm, 2 to 50 atm, 5 to 50 atm Atmospheric pressure, 7 atm to 50 atm, 10 atm to 50 atm, 15 atm to 50 atm, 1.5 atm to 30 atm, 2 atm to 30 atm, 7 atm to 50 atm, 10 atm to 50 atm, 1.5 atm to 30 atm, It may be performed under a pressure of 2 to 30 atm, 5 to 30 atm, 7 to 30 atm, 10 to 30 atm, or 15 to 30 atm.

The microwave irradiation is 110 ℃ to 150 ℃, for example, 110 ℃ to 140 ℃, 110 ℃ to 130 ℃, 110 ℃ to 120 ℃, 120 ℃ to 150 ℃, 120 ℃ to 140 ℃, 120 ℃ to 130 ℃ , 130°C to 150°C, 130°C to 140°C, or 140°C to 150°C.

The microwave irradiation is 30 minutes to 90 minutes, 30 minutes to 80 minutes, 30 minutes to 70 minutes, 30 minutes to 60 minutes, 30 minutes to 50 minutes, 30 minutes to 40 minutes, 40 minutes to 90 minutes, 40 minutes to 80 minutes, 40 minutes to 70 minutes, 40 minutes to 60 minutes, 40 minutes to 50 minutes, 50 minutes to 90 minutes, 50 minutes to 80 minutes, 50 minutes to 70 minutes, 50 minutes to 60 minutes, 60 minutes to 90 minutes , 60 minutes to 80 minutes, 60 minutes to 70 minutes, 70 minutes to 90 minutes, 70 minutes to 80 minutes, or may be performed for 80 minutes to 90 minutes.

The microwave irradiation may be performed under pressure at 110° C. to 150° C. for 30 to 90 minutes. The microwave irradiation may be performed at 110° C. to 150° C. for 30 to 90 minutes under 2 to 100 atmospheres. The microwave irradiation may be performed at 120° C. to 150° C. for 30 to 90 minutes under 2 to 100 atmospheres. The microwave irradiation may be performed at 120° C. to 150° C. for 30 to 90 minutes. The microwave irradiation may be performed at a pH of 5.0 to 7.0, a pH of 5.0 to 6.0, or a neutral pH.

Another aspect provides an extract of the genus Panax plant prepared by the above method.

Another aspect provides a pharmaceutical composition comprising an extract of the genus Panax plant prepared by the above method. The pharmaceutical composition is an anticancer agent, for example, inhibiting resistance to taxol and skin protection, antidiabetic, anticancer (e.g., anti-lung cancer, anti-liver cancer), anti-inflammatory, antioxidant, inhibiting platelet aggregation, or a pharmaceutical for improving skin It may be a composition. The pharmaceutical composition may further include a pharmaceutically acceptable carrier or diluent.

The extract has a remarkably higher content of ginsenosides Rg6, Rk3 and Rh4 compared to the simple heat treatment product, and thus has increased efficacy of ginsenosides Rg6, Rk3 and Rh4. Ginsenoside Rg6 is known to inhibit resistance to anticancer drugs, skin protection, antidiabetic, and anticancer effects, and ginsenoside Rk3 inhibits the proliferation of lung and liver cancer cell lines, induces apoptosis, and has anti-inflammatory and antioxidant activities. And ginsenoside Rh4 is known to have anti-platelet aggregation, anti-inflammatory activity, and skin protection. Therefore, the extract may have such an effect significantly increased compared to a simple heat treatment product.

Another aspect provides a food composition comprising a plant extract of the genus Panax prepared by the above method.

The food may be a functional food or a health functional food. The food composition may further include a food-acceptable carrier or diluent.

Another aspect provides a cosmetic composition for improving skin wrinkles comprising the plant extract of the genus Panax prepared by the above method. The cosmetic composition may be a formulation suitable for skin administration, for example, a lotion.

According to the production method of the plant extract of the genus Panax according to one aspect, total ginsenosides Rg1, Re, Rh1, Rg2, Rb1, Rc, Rb2, Rd, Rg6, Rk3, Rh4, S -Rg3, R -Rg3, Rk1, And it is possible to efficiently produce a plant extract of the genus Panax containing 20% or more by weight of rare ginsenosides Rg6, Rk3 and Rh4 based on the weight of Rg5.

According to the pharmaceutical composition comprising the plant extract of the genus Panax according to another aspect, inhibition of resistance to anticancer agents, skin protection, antidiabetic, anticancer (eg, anti-lung cancer, anti-liver cancer), anti-inflammatory, antioxidant, platelet aggregation It can be used for suppressing or improving skin wrinkles.

According to the composition for food containing the plant extract of the genus Panax according to another aspect, it can be used as a food, particularly a functional food.

According to the cosmetic composition comprising the plant extract of the genus Panax according to another aspect, it can be used for improving skin wrinkles.

1 is a UPLC chromatogram analyzing the ginsenoside component of the raw ginseng leaf extract.
FIG. 2 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 110° C. and 100 W for 30 minutes.
3 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 30 minutes.
4 is a UPLC chromatogram analyzing the ginsenoside component in the microwave irradiated product obtained by microwave processing of ginseng leaf extract at 130° C. and 100 W for 30 minutes.
5 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 140° C. and 100 W for 30 minutes.
6 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 150° C. and 100 W for 30 minutes.
7 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 40 minutes.
8 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 50 minutes.
9 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 60 minutes.
10 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 70 minutes.
11 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 80 minutes.
12 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 90 minutes.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of ginseng leaf extract

Panax ginseng leaf used in this example was harvested in July and used in an area grown in Ansan-si, Gyeonggi-do, Korea. 2 L of 50% ethanol was added to 100 g of minced ginseng leaves, followed by heating under reflux extraction at about 80° C. for 2 hours to obtain a 50% ethanol extract, and the obtained 50% ethanol extract was evaporated to dryness under reduced pressure to evaporate the ginsenoside Rg1. , Re, Rb1, Rc, Rb2 and Rd to obtain 14.5 g of a dry extract containing the content of Rg1 and Re is 55% by weight or more.

Example 2-7: Processing of ginseng leaf extract using microwave (1)

The 50% ethanol extract obtained in Example 1 was subjected to microwave heat treatment. Specifically, 200 mg of the dried ginseng leaf extract obtained in Example 1 was added to a 10 mL container of a microwave irradiator manufactured by CEM (Model No. 908005), along with 1 mL of a 50% ethanol aqueous solution, and the container was sealed. At 100 W (frequency 2455 MHz) for about 30 minutes at 110° C. (Example 2), 120° C. (Example 3), 130° C. (Example 4), 140° C. (Example 5), and 150° C. (Example 6) ), and then freeze-dried to obtain a microwave-irradiated workpiece. The pressure during microwave irradiation was 20 atmospheres. The pH of the reactant at the initial stage of microwave irradiation was 5.7.

Example 8-13: Processing of ginseng leaf extract using microwave (2)

The 50% ethanol extract obtained in Example 1 was subjected to microwave heat treatment. Specifically, 200 mg of the dried ginseng leaf extract obtained in Example 1 was added together with 1 mL of 50% ethanol to a 10 mL container of a microwave irradiator (model number 908005) manufactured by CEM of the United States, and the container was sealed. 40 minutes (Example 7), 50 minutes (Example 8), 60 minutes (Example 9), 70 minutes (Example 10), 80 minutes (Example 11) at 100 W (frequency 2455 MHz) at 120°C , After each microwave irradiation for 90 minutes (Example 12), lyophilization was performed to obtain a microwave irradiated workpiece. The pressure during microwave irradiation was 20 atmospheres.

Experimental Example 1: Analysis of ginsenosides of microwave workpieces

(1) Experiment method

Each saponin component of the processed ginseng leaf extract obtained in the above Example was analyzed based on the verification line values using ginsenoside Rg6, Rk3 and Rh4 standards. Saponin analysis was repeated three times to confirm reproducibility. The elution solvent A used in Ultra Performance Liquid Chromatography (UPLC) was water, and the elution solvent B was acetonitrile, and each was pumped using two pumps. 3 µl of the standard aqueous solution was injected into a reverse phase column (C18, 2.1 X 150 mm) which is a separation column using a syringe, and an elution solvent having a composition of 85 vol% A and 15 vol% B was added to 0.5 mL/ Flowed at the flow rate of minutes. After that, the volume% of the elution solvent B is 30% (28 minutes), 32% (30 minutes), 38% (36 minutes), 43% (47 minutes), 55% (54 minutes), 70% (70 minutes) ), gradually changed to 90% (76 minutes) and the composition was maintained for 6 minutes. After the above process, each component separated from the column was analyzed through a UV spectrum.

(2) Experiment result

As a result of the experiment, as a result of analyzing each component separated from the column by UPLC analysis for the processed ginseng leaf extract, the peaks of FIGS. 1-12 were obtained. In Fig. 1, the horizontal axis represents time (minutes), and the vertical axis represents an arbitrary unit (AU).

1 is a UPLC chromatogram analyzing the ginsenoside component of the raw ginseng leaf extract. Hereinafter, "raw ginseng leaf extract" refers to the water or 50% ethanol aqueous solution extract obtained in Example 1.

FIG. 2 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 110° C. and 100 W for 30 minutes.

3 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 30 minutes.

FIG. 4 is a UPLC chromatogram analyzing ginsenoside components in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 130° C. and 100 W for 30 minutes.

5 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 140° C. and 100 W for 30 minutes.

6 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 150° C. and 100 W for 30 minutes.

7 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 40 minutes.

8 is a UPLC chromatogram analyzing the ginsenoside component in the microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 50 minutes.

9 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 60 minutes.

10 is a UPLC chromatogram analyzing the ginsenoside component in the microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 70 minutes.

11 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 80 minutes.

12 is a UPLC chromatogram analyzing the ginsenoside component in a microwave irradiated product obtained by microwave processing a ginseng leaf extract at 120° C. and 100 W for 90 minutes.

In addition, the results of calculating each ginsenoside content obtained from the obtained UPLC chromatogram are summarized in Tables 1 and 2.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Rg1 43.5 20.6 15.4 4.1 - - 23.6 10.9 - - - - Re 52.6 24.7 19.0 5.4 - - - - - - - - Rh1 - 16.8 10.3 5.2 2.2 1.5 14.0 5.9 4.8 4.2 2.5 - Rg2 - 6.5 4.1 2.3 0.5 - 5.4 2.4 1.5 1.2 0.9 - Rb1 22.3 18.7 11.3 - - - - - - - - - Rc 20.8 7.9 6.9 - - - 3.0 - - - - - F1 - - - - - - - - - - - - Rb2 12.1 6.2 7.0 - - - 6.2 4.5 - - - - Rd 21.0 18.8 8.8 - - - - - - - - - Rg6 - 7.2 12.6 15.6 9.0 4.0 23.6 16.5 12.1 9.1 7.1 5.7 Rk3 - 3.4 10.0 13.0 21.0 24.4 20.1 26.7 22.7 19.8 15.1 11.9 Rh4 - 4.6 11.5 15.2 27.6 34.8 22.3 30.0 36.3 31.0 23.3 18.2 F2 - - - - - - - - - - - - S-Rg3 - 5.1 8.6 10.1 5.5 1.7 6.1 5.2 1.9 1.3 0.6 0.4 R-Rg3 - 6.9 9.6 10.5 9.0 3.3 9.0 8.5 7.1 5.9 3.1 2.1 C-Y - - - - - - - - - - - - C-K - - - - - - - - - - - - Rk1 - 8.2 7.4 8.8 8.3 6.4 7.2 7.2 4.6 2.9 1.5 1.3 Rg5 - 4.9 10.8 9.9 9.9 8.1 9.0 7.7 6.1 5.9 3.3 - Rh2 - - - - - - - - - - - - Rk2 - - - - - - - - - - - - Rh3 - - - - - - - - - - - - Total 172.3 160.2 153.3 100.1 93.0 84.2 149.5 125.5 97.1 81.3 57.4 38.3

Content of Rg6+Rk3+Rh4 in total ginsenoside content (% by weight ) Example 1 0.0 Example 2 9.5 Example 3 22.2 Example 4 43.8 Example 5 61.9 Example 6 75.1 Example 7 44.2 Example 8 58.3 Example 9 73.2 Example 10 73.7 Example 11 79.3 Example 12 93.5

Table 3 shows the weight percent of total ginsenosides of R6, Rk3 and Rh4.

Ex 1 Ex 2 Ex 3 Ex 4 Ex 5 Ex 6 Ex 7 Ex 8 Ex 9 Ex 10 Ex 11 Ex 12 Rg6 0 4.5 8.2 15.6 9.7 4.8 15.8 13.1 12.5 11.2 12.4 14.9 Rk3 0 2.1 6.5 13.0 22.6 29.0 13.4 21.3 23.4 24.4 26.3 31.1 Rh4 0 2.9 7.5 15.2 29.7 41.3 14.9 23.9 37.4 38.1 40.6 47.5

In Tables 1 and 2, each number represents mg of ginsenoside per gram of the processed product. As shown in Examples 2 to 6 of Table 1 and Table 2, the main ginsenosides Rg1 and/or Re contained in the ginseng leaf extract (Example 1) were heat treated at 120° C. to 150° C. for 30 minutes. As a result, it was found that glucose, which is a glycoside at position 20, was released, and then a dehydration reaction occurred at position 20 and converted into Rg6, Rk3 and Rh4.

As shown in Examples 3 and 7 to 12 of Tables 1 and 2, in the case of microwave irradiation at 120° C. for 30 to 90 minutes, the content ratio of ginsenosides Rg6, Rk3 and Rh4 is microwave irradiation time Showed a tendency to increase in proportion

According to the above results, when the ginseng leaf extract was irradiated with microwave, it was possible to more efficiently convert the main ginsenosides Rg1 and Re of ginseng into Rg6, Rk3 and Rh4, which are excellent or alternative ginsenosides. In addition, as shown in Table 2, a microwave irradiated product containing 9.5% or more, 22.2% or more, or 43.8% or more of Rg6, Rk3 and Rh4 with respect to the total ginsenoside weight was obtained. In particular, it can be seen that the content of ginsenosides Rg6, Rk3 and Rh4 is significantly higher than that of the simple heat treatment process developed so far. This effect is an unexpected and remarkable effect compared to the prior art.

Claims (18)

A plant extract of the genus Panax containing 40% or more of the weight of rare ginsenosides Rg6, Rk3 and Rh4 based on the total ginsenosides obtained by irradiating the leaves or extracts thereof with microwaves, total ginsenosides Is an extract that represents Rg1, Re, Rh1, Rg2, Rb1, Rc, Rb2, Rd, Rg6, Rk3, Rh4, S-Rg3, R-Rg3, Rk1, and Rg5,
The microwave irradiation is an extract performed for 50 minutes to 90 minutes at 110 ℃ to 130 ℃ under 2 atmospheres to 100 atmospheres,
The weight of Rg6 is 10% by weight or more based on the total ginsenoside weight, the weight of Rk3 is 14% by weight or more based on the total ginsenoside weight, and the weight of Rh4 is 16% by weight based on the total ginsenoside weight. Panax genus plant extract that is the above. The plant extract of the genus Panax according to claim 1, wherein in the "leaf of the genus Panax or an extract thereof", the extract is an extract of water, C1-C6 alcohol, or a mixture thereof of the leaves of the genus Panax. The plant extract of claim 2, wherein the alcohol extract comprises the step of contacting the leaves of the genus Panax with water, C1-C6 alcohol, or a mixture thereof at 50° C. to reflux temperature. The plant extract of claim 1, wherein the microwave irradiation is performed at pH 5.0 to 7.0. The method according to claim 1, wherein the plant of the genus Panax is Korean ginseng (Panax ginseng), Hwagi ginseng (Panax quinquefolia), Jeonchil ginseng (Panax notoginseng), Jukjeolsam (Panax japonica), Trifolia (Panax trifolia), Himalayan ginseng (Panax pseudoginseng), Vietnamese ginseng (Panax vietnamensis), or a combination thereof, Panax genus plant extract. delete delete delete delete delete delete A food composition comprising the plant extract of the genus Panax according to any one of claims 1 to 5. Method for producing a plant extract of the genus Panax containing 40% or more by weight of rare ginsenosides Rg6, Rk3 and Rh4 based on the total weight of ginsenosides comprising the step of irradiating the leaves of the genus Panax or extracts thereof with microwaves As, the total ginsenosides are Rg1, Re, Rh1, Rg2, Rb1, Rc, Rb2, Rd, Rg6, Rk3, Rh4, S-Rg3, R-Rg3, Rk1, and Rg5.
The microwave irradiation is a method performed for 50 minutes to 90 minutes at 110 ℃ to 130 ℃ under 2 atmospheres to 100 atmospheres,
The weight of Rg6 is 10% by weight or more based on the total ginsenoside weight, the weight of Rk3 is 14% by weight or more based on the total ginsenoside weight, and the weight of Rh4 is 16% by weight based on the total ginsenoside weight. The way it is more. The production method according to claim 13, wherein in the "leaf of the genus Panax or an extract thereof", the extract comprises 55% by weight or more of Rg1 and Re based on the total ginsenosides of the leaves of the genus Panax. The production method according to claim 13, wherein in the "leaf of the genus Panax or an extract thereof", the extract is an extract of water, C1-C6 alcohol, or a mixture thereof of leaves of the genus Panax. The method according to claim 13, wherein the plant of the genus Panax is Korean ginseng (Panax ginseng), Hwagi ginseng (Panax quinquefolia), Jeonchil ginseng (Panax notoginseng), Jukjeolsam (Panax japonica), Trifolia (Panax trifolia), Himalayan ginseng (Panax pseudoginseng), Vietnamese ginseng (Panax vietnamensis), or a combination thereof, the production method. The production method according to claim 13, wherein the microwave irradiation is performed at a pH of 5.0 to 7.0. The production method according to claim 13, wherein the microwave irradiation is performed in a solution of the leaves of the genus Panax or water containing an extract thereof, C1-C6 alcohol, or a mixture thereof.

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