KR102171032B1 - Method for producing propolis extract using a stirring device having filtration characteristics - Google Patents

Abstract

The present invention relates to a method for manufacturing a propolis extract capable of improving extracting efficiency by improving a shape of a stirring device provided in a stirring apparatus and the number of stirring devices, and forming water-soluble propolis by using the stirring apparatus having filtration characteristics; not using an emulsifier when mixing with other raw materials; and providing a useful effect of providing the excellent extracting efficiency without applying a physical factor such as ultrasonic waves through improvement of a stirring rate. The present invention provides a useful effect of preserving nutrients of the propolis by performing a process at 20-60°C and minimizing loss by heat. The method for manufacturing a propolis extract comprises: a step of manufacturing a propolis filtering liquid; a step of manufacturing a propolis alcohol extracting liquid; and a step of manufacturing the propolis extract.

Description

Manufacturing method of propolis extract using a stirring device having filtration characteristics {METHOD FOR PRODUCING PROPOLIS EXTRACT USING A STIRRING DEVICE HAVING FILTRATION CHARACTERISTICS}

The present invention relates to a method for producing a propolis extract, and specifically, to a method for producing a propolis extract having water-soluble properties using a stirring device having a filtering property.

Propolis is a dendritic protective material collected from numerous plant skins to prevent rainwater from seeping by bees filling the gaps in the hive and to block the hive safely from the outside. It contains a lot of organic matter and minerals. Since it contains more flavonoids, it has antibacterial and antioxidant effects, and various products are manufactured in the form of propolis extracts.

Specifically, the propolis extract refers to an extract obtained by removing wax from propolis made by bees using the sap of a tree, pollen collected from a flower's cancer and surgery, and the secretions of bees themselves.

While the demand for propolis is increasing in recent years, it is difficult to use propolis for various purposes due to its insoluble in water, and to solve this problem, a technology of extracting propolis using ethanol, water, glycerin and emulsifier is used. However, there is a problem that the quality of the product is deteriorated due to the low recovery rate and the remaining wax components, and the content of flavonoids, which is an active ingredient of propolis, is very low, making it difficult to exhibit antibacterial and antioxidant functions, which are the original functions of propolis.

Accordingly, in the industry, there is a situation in which the development of a technology capable of reducing the ratio of the remaining wax components and obtaining a propolis extract having a high content of active ingredients such as flavonoids is required.

Accordingly, the applicant of the present application intends to propose the following propolis extraction technology, which is further improved compared to the existing propolis extraction technologies and has excellent separation efficiency of active ingredients without using a separate emulsifier, and in particular, while reducing the stirring time, It is intended to propose a method of preparing a propolis extract having a high active ingredient.

Republic of Korea Patent Publication No. 10-2006-0040226

An object of the present invention is to solve the above-described conventional problems and to provide a method for preparing a propolis extract having water-soluble and high active ingredients while reducing the stirring time.

The present invention (A) in order to remove the wax and impurities from the propolis, the steps of preparing a propolis filtrate by mixing and stirring the propolis and a solvent, followed by filtration;

(B) (A) the wax and impurities removed from the propolis filtrate and alcohol were added to a stirring device and stirred for 2 to 10 hours at a temperature of 20°C to 60°C and 500 to 1000 rpm. Preparing an alcohol extract; And

(C) mixing and stirring the propolis alcohol extract with distilled water and L-arginine, storing at a low temperature at a temperature of 5°C to 10°C, and filtering to prepare a propolis extract; including,

The stirring device,

A treatment tank manufactured in a triple structure and provided with an inlet for introducing the propolis filtrate and alcohol; A stirrer installed in the treatment tank to stir the propolis filtrate and alcohol; A cooling means for recovering the evaporation of ethanol generated from the treatment tank into the inside of the treatment tank during extraction of propolis; And a temperature control means for maintaining the temperature in the treatment tank in a constant state,

The agitator includes a stirring motor, a stirring shaft mounted on the stirring motor, and a plurality of stirring blades mounted on the stirring shaft, and the stirring blade is a structure in which two or more filters having a mesh structure are stacked. It provides a method for producing a propolis extract.

According to the present invention, the shape and number of stirrers provided in the stirrer are improved to form a water-soluble propolis using a stirrer having filtration characteristics to improve extraction efficiency, and when mixing with other raw materials, an emulsifier, etc. And, it is possible to achieve a useful effect showing excellent extraction efficiency without adding physical elements such as ultrasonic waves through the improvement of the stirring rate.

In addition, according to the present invention, it is possible to achieve a useful effect of preserving the nutrients of propolis by minimizing heat loss by performing the process at about 20°C to 60°C.

1 is a flow chart showing a method for producing a propolis extract according to an embodiment of the present invention.
Figure 2 is a schematic diagram showing a stirring device used in the method for producing a propolis extract according to an embodiment of the present invention.

A preferred embodiment of the present invention will be described with reference to the accompanying drawings, and it will be possible to better understand the objects and configurations of the present invention, and features thereof, through such detailed description.

The method for preparing a propolis extract according to an embodiment of the present invention uses a stirring device including a stirring blade in which a plurality of mesh-structured filters are stacked to improve the extraction efficiency of water-insoluble propolis without a separate emulsifier. Water-soluble propolis can be extracted.

Hereinafter, while mainly describing the stirring device used for extracting propolis in the present invention, compared to the conventional propolis extraction method, it is not necessary to use an emulsifier or the like when mixing with other raw materials. It is intended to propose a method for producing a propolis extract that exhibits excellent extraction efficiency even without adding urea.

To this end, the method for producing a propolis extract according to an embodiment of the present invention, as shown in Figure 1, the propolis filtrate production step (S100), the propolis alcohol extract production step (S200) and the propolis extract production step ( S300).

The propolis filtrate preparation step (S100) is a step for removing wax and impurities from the propolis after dissolving the propolis original mass in a solvent. At this time, the solvent may be alcohol or distilled water.

Specifically, the propolis filtrate manufacturing step (S100) is a step of separating a water-insoluble resinous material, wax and impurities contained in the propolis original mass, and forming a uniform solution by mixing the propolis original mass and a solvent In order to do so, it may be stirred at a temperature of 40°C to 60°C at 300 to 500 rpm for 5 to 12 hours.

After filtering the stirred solution using a screen filter, the filtered solution is maintained at a low temperature at 5°C to 10°C, and then wax and impurities are removed to prepare a propolis filtrate.

At this time, the screen filter serves to adjust the size of the propolis in the stirred solution, and when kept in a low-temperature storage state, the solidified resinous material, wax, and impurities can be easily separated.

Specifically, the solidified resinous material, wax, and impurities may be removed by using a micro filter in a low temperature state of 5°C to 10°C in the stirred solution filtered through the screen filter. In this case, the micro filter may be a filter having a size of 100 μm or less.

When the solution obtained by stirring the propolis ingot and the solvent is separated by filtration at room temperature, the filtration efficiency is low due to the viscosity of resinous material and wax, making it difficult to completely remove impurities such as resinous material and wax. Solidified resinous substances, waxes and impurities can be separated from the solution by storing a homogeneous solution in which the and solvent are dissolved under heating and stirring at a low temperature, and it can be completely separated and removed from the solution, and has a high content of propolis. There is a characteristic that is accepted as

The propolis alcohol extract manufacturing step (S200) is a step of preparing a concentrated propolis alcohol extract from the propolis filtrate by stirring the propolis filtrate and alcohol using a stirring device.

Here, the propolis alcohol extract manufacturing step (S200) will be described mainly on a method of stirring using a stirring device.

As shown in Figure 2, the stirring device is manufactured in a triple structure consisting of a main body, a jacket, and a cover, and a treatment tank 10 provided with an inlet 11 for introducing the propolis filtrate and alcohol; Agitator mounted in the treatment tank 10 to stir the propolis filtrate and alcohol, but mounted on the stirring shaft 21 of the stirrer and including agitation blades 22 to 25 in which a filter having a mesh structure is stacked ; Cooling means for recovering the evaporation of ethanol generated from the treatment tank 10 into the inside of the treatment tank 10 during extraction of propolis, and temperature control to maintain the temperature in the treatment tank 10 at a constant state Includes means.

The treatment tank 10 is a treatment tank for agitating the propolis filtrate and alcohol, and an inlet 11 for inputting raw materials is provided in the upper part of the treatment tank 10, and agitation is performed in the lower part of the treatment tank 10. An outlet 14 for discharging the finished propolis alcohol extract is provided.

The treatment tank 10 is made of SUS and has a triple structure, and a jacket is installed between the inner and the outer body to maintain a constant temperature inside the treatment tank 10 to fill the jacket with a heat conducting medium 14. have. The treatment tank 10 applies electricity to the heat transfer medium 14 having excellent thermal conductivity by supplying electricity from the heater 60 of the temperature control means provided outside by applying an electric method so that the desired temperature can be quickly reached. .

At this time, the temperature of the treatment tank is maintained at a temperature of 20°C to 60°C so that the active ingredient of propolis can be contained in a high content. By controlling the temperature of the treatment tank to about 30° C. to 50° C. in this way, nutrients can be completely preserved by minimizing heat loss in the stirring process.

Outside the treatment tank 10, as shown in FIG. 2, a plurality of supports 13 for holding the treatment tank 10 are provided.

The agitator includes a stirring motor 20, a stirring shaft 21 mounted on the stirring motor 20, a plurality of stirring blades 22-25 mounted on the stirring shaft 21, and the stirring Yong motor 40 rotates the plurality of stirring blades (22 to 25) at a speed of 500 to 1000 rpm/min.

The plurality of stirring blades (22-25) are provided along the stirring shaft (21) of the stirrer, the stirring blade is provided with 2 to 5 stirring blades at the same height based on the stirring shaft (21), the stirring shaft The width and length of the stirring blades 22 to 25 may gradually increase from the top to the bottom of (21). Specifically, the angle of the stirring blade is not particularly limited, but may be provided so that the end of the stirring blade is lower than the horizontal. In this way, when the size of the stirring blade increases toward the bottom along the stirring shaft 21, when the propolis filtrate and alcohol are stirred in the treatment tank, the stirring efficiency of the lower part where relatively heavy substances collect is improved to form a uniform solution. Can, and the stirring time can be reduced.

The plurality of stirring blades 22 to 25 is a structure in which two or more filters having a mesh structure are stacked. By including a filter having a mesh structure as described above, a plurality of stirring blades 22 to 25 rotate by the stirring shaft 21 to uniformly mix the mixed solution in the treatment tank 10 and at the same time, By filtering the mixed solution, a uniform solution may be formed by reducing the size of the propolis in the mixed solution.

In addition, in the filter provided in the plurality of stirring blades 22 to 25, the size of the hole of the filter gradually increases from the top to the bottom of the stirring shaft 21. In this way, as the size of the filter hole formed in the stirring blade increases toward the bottom along the stirring shaft 21, the relatively heavy propolis collected in the lower portion of the treatment tank is efficiently filtered to form a water-soluble propolis, smoothly without an emulsifier. Let it stir.

The geared motor applied to the present invention is provided with a stirring motor 20 so as to rotate a plurality of stirring blades (22 ~ 25) at a speed of 500 ~ 1000rpm / min to rotate the stirring shaft (21). At this time, a variable resistor is installed to change the rotation, so that the optimum number of stirring rotations required to maximize the extraction efficiency can be set through a test, and the geared motor used at this time is, for example, 3hp by 1/30 deceleration. do.

The cooling means is provided in the upper portion of the treatment tank 10 and includes a condenser 30 for cooling the evaporation of ethanol in the treatment tank 10 and a chiller 40 for supplying cooling water to the condenser 30. Include.

The condenser 30 is used in the treatment tank 10 to recover the evaporation of ethanol generated by increasing the temperature and pressure by stirring inside the treatment tank 10 during extraction of propolis by condensation through heat exchange. It is provided with a heat exchange pipe mounted on the top. That is, the ethanol evaporating in the treatment tank 10 contains a propolis component and is provided as a recovery device for the original member in order to reduce cost, and the cooling water cooled by the chiller 40 is supplied to perform heat exchange.

The chiller 40 is a device that cools to supply the cooling water of the condenser 30, and it is preferable to install at least 5 to 7 hp.

The temperature control means is a temperature sensor 50 that senses the temperature in the treatment bath 10, and maintains the temperature in the treatment bath 10 at 20°C to 60°C according to the temperature detected by the temperature sensor 50. It includes a heater 60 and a controller (not shown) for controlling the operation of the heater 60 according to the temperature sensed by the temperature sensor 50.

In the present invention, in order to maintain the temperature in the treatment tank 10 at 20°C to 60°C, preferably 30°C to 50°C, the heater 60 includes a heat conductive medium 14 provided in the jacket of the treatment tank 10. Operate by electricity to achieve the proper extraction temperature. At this time, the heat conduction medium 14 is not particularly limited, but palm oil may be used.

The operation of the heater 60 can be performed automatically according to monitoring by the control unit by installing a temperature sensor 50 on the side of the treatment tank 10, as shown in FIG. 2.

In the propolis alcohol extract manufacturing step (S200), the propolis filtrate and alcohol are stirred for 2 to 10 hours at a temperature of 20° C. to 60° C. and a condition of 500 to 1000 rpm using the same stirring device. The extract can be prepared.

In the propolis alcohol extract manufacturing step (S200), the propolis alcohol extract prepared through the stirring device may be further filtered using a micro filter. In this case, the micro filter may be a filter having a size of 100 μm or less. By filtering using a micro filter as described above, wax and impurities present in the propolis alcohol extract can be removed, and particles of propolis can be reduced.

In the propolis extract manufacturing step (S300), the extracted propolis is water-soluble and the last remaining impurities are removed.

In the propolis extract manufacturing step (S300), auxiliary ingredients such as propolis alcohol extract, distilled water, and L-arginine may be stirred and uniformly mixed to be aged and stabilized for 1 to 3 days.

In the propolis extract manufacturing step (S300), the aged and stabilized extract is stored at a low temperature at a temperature of 5°C to 10°C, and then filtered through a micro filter to obtain a propolis extract having a content of 40% to 70% by weight. Can be manufactured.

At this time, the micro-filter may have an impurity removal yield of 90% or more by using a filter having a size of 0.1 μm to 10 μm, and the stability of the product may always be improved by using a natural filtration method. In addition, when maintained in a low-temperature storage state, the solidified resinous material, wax, and impurities can be easily separated.

When the solution obtained by mixing the propolis ingots with a solvent is separated by filtration at room temperature, the filtration efficiency is low due to the viscosity of resinous materials and waxes, making it difficult to completely remove impurities such as resinous materials and waxes. Solidified resinous substances, waxes and impurities can be separated from the solution by storing a homogeneous solution in which the and solvent are dissolved under heating and stirring at a low temperature, and it can be completely separated and removed from the solution, and has a high content of propolis. There is a characteristic that is accepted as

S100: Propolis filtrate manufacturing step
S200: Propolis alcohol extract manufacturing step
S300: Propolis extract manufacturing step
10: treatment tank, 11: inlet, 12: outlet, 13: support, 14: heat conduction medium
20: stirring motor, 21: stirring shaft, 22 to 25: stirring blade
30: condenser, 40: chiller,
50: temperature sensor, 60: heater

Claims (6)

(A) in order to remove wax and impurities from the propolis, the propolis original mass and the solvent are mixed, stirred, and filtered to prepare a propolis filtrate;
(B) (A) the wax and impurities removed from the propolis filtrate and alcohol were added to a stirring device and stirred for 2 to 10 hours at a temperature of 20°C to 60°C and 500 to 1000 rpm. Preparing an alcohol extract; And
(C) mixing and stirring the propolis alcohol extract with distilled water and L-arginine, storing at a low temperature at a temperature of 5°C to 10°C, and filtering to prepare a propolis extract; including,
The stirring device,
A treatment tank manufactured in a triple structure and provided with an inlet for introducing the propolis filtrate and alcohol; A stirrer installed in the treatment tank to stir the propolis filtrate and alcohol; Cooling means for recovering the evaporation of ethanol generated from the treatment tank into the inside of the treatment tank during extraction of propolis; And a temperature control means for maintaining the temperature in the treatment tank at a constant state,
The agitator includes a stirring motor, a stirring shaft mounted on the stirring motor, and a plurality of stirring blades mounted on the stirring shaft, and the stirring blade is a structure in which two or more filters having a mesh structure are stacked,
The stirrer is provided with a plurality of stirring blades along the stirring shaft, and the width and length of the stirring blades gradually increase from the top to the bottom of the stirring shaft,
The filter provided in the stirring blade is a method for producing a propolis extract, characterized in that the size of the filter hole gradually increases from the top to the bottom of the stirring shaft. The method of claim 1,
Step (A) is,
(a) the process of stirring the propolis original mass and the solvent to a uniform solution at a temperature of 20 ℃ to 60 ℃;
(b) filtering the solution stirred in step (a) using a screen filter; And
(c) a method for producing a propolis extract comprising the step of removing the wax and impurities after maintaining a low temperature state of the solution filtered in the step (b) at 5 ℃ to 10 ℃. The method of claim 1,
In step (B), the propolis filtrate and alcohol are added to the stirring device and stirred by a stirrer in the stirring device, and a low-molecular propolis is formed by a stirring blade equipped with a filter having a mesh structure of the stirrer. Method for producing a propolis extract, characterized in that to form a solution. delete delete The method of claim 1,
Step (C) is,
(a) mixing and stirring the propolis alcohol extract, distilled water and L-arginine; And
(b) The solution stirred in step (a) is stored at a low temperature at a temperature of 5°C to 10°C, and then filtered through a micro filter to prepare a propolis extract having a content of 40% by weight to 70% by weight. Method for producing a propolis extract containing.

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