KR102164119B1 - A low irritating antimicrobial composition and a cosmetic composition comprising the same - Google Patents

Abstract

Provided is an antibacterial composition comprising ethylhexyl glycerin, caprylyl glycol, methylpropanediol, and a surfactant. The antibacterial composition is hypoallergenic and safe by using only raw materials with EWG score 1, has a synergistic antibacterial effect compared to efficacy of each component by using a combination of four components, has a broad spectrum for microorganisms, and thus exhibits excellent antibacterial effects even at low concentrations. Therefore, a cosmetic composition having high preservation power and safety can be prepared by using the antibacterial composition.

Description

Hypoallergenic antibacterial composition, cosmetic composition comprising the same {A LOW IRRITATING ANTIMICROBIAL COMPOSITION AND A COSMETIC COMPOSITION COMPRISING THE SAME}

The present invention relates to a hypoallergenic antibacterial composition and a cosmetic composition comprising the same.

In general, the cause of decay, deterioration, or rancidity in cosmetic ingredients and products is caused by contamination of microorganisms, and the microorganisms include fungi of fungi, yeast and bacteria, and kills bacteriostatics and microorganisms that block the growth of these microorganisms. Preservatives and antibacterial agents are responsible for letting go. Formulations with antiseptic and antibacterial activity are widely used not only in cosmetics but also in industrial products, foods, and pharmaceuticals.

In particular, the safety issue of preservatives used in cosmetics has emerged. In general, most of the substances used as preservatives are chemically synthesized, for example, chloromethylisothiazolinone (CMIT), polyhexamethylene guanidine (PHMG), polyhexamethylene biguanide (PHMB), parabens, Imidazolidinyl urea, diazolidinyl urea, and the like are used as antibacterial and preservative agents.

In general, because of the broad spectrum of microorganisms, the most widely used preservatives are Para-hydroxybenzoic acid esters (Paraben). Paraben-type preservatives are commonly used as methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isopropyl paraben, and isobutyl paraben. They are used alone because of their different solubility and permeability in water, and the spectrum of microorganisms. It has been used interchangeably. However, paraben-like preservatives quickly pass through the skin, are absorbed into the intestine, and then discharged into the urine, and some may remain in the body and interfere with the metabolism of the estrogen hormone. It can remain in the amniotic fluid and placenta of pregnant women, and when it remains in the breast tissue of a woman, it can act as a factor that increases the potential risk of breast cancer. In addition, the parabens preservative has problems such as causing skin allergy and resistant bacteria. Parabens are among the most inexpensive and have strong efficacy even in small amounts, but the amount of use is decreasing worldwide for the same reason, especially in Korea, which is rarely used.

Next, the preservative that has been widely used in cosmetics and personal care is 2-Phenoxy Ethanol (2-PE). Patent Document 1 discloses a chemical composition comprising a preservative system containing iodopropynyl butyl carbamate and phenoxyethanol. 2-Phenoxyethanol is generally used in a content of less than 1%, but it has a characteristic odor, which affects the product and can cause skin irritation and allergies, so pregnant women should avoid it, especially when exposed to infants, damage the central nervous system. Can give For these safety reasons, the use of 2-PE was also reduced in personal care products. So, the proportion of using 1,2-hexanediol (1,2-Hexanediol) has increased, but it also has a characteristic odor, and it is generally used in a high content of 2%, so the cost ratio is high and the microbial spectrum is not wide. Recently, there are many places to replace.

In addition, guanine-based substances such as PHMG and PHMB are a causative agent of acute lung injury syndrome caused by a disinfectant for humidifiers, and there are many problems, such as a risk issue, and other substances are also classified as factors causing dermatitis.

As described above, the danger of such artificial preservatives is emerging, and recently, cosmetics and food industries are replacing substances with minimal toxicity to the human body, and research has been emerging to replace various chemical substances with natural substances.

However, natural materials undergo a chemical process using a solvent during refining, and their inherent scent or color remains, making it difficult to use in many fields, and they are not evenly dispersed in actual formulations than in the experimental results, and their stability is poor. It is difficult to do or use it in excessive amounts, so you can expect efficacy. In addition, it can have strong antimicrobial activity in laboratory tests, but products that are actually manufactured are made with a combination of various ingredients.When applied to these actual formulations and tested (challenge test), they do not clump or disperse, so they are effective in challenge tests. Most of the cases of loss or dropping will occur. In order to overcome this point, a large amount must be used, but the cost of natural materials is high, making it difficult to apply in practice.

Here, the challenge test will be described. In the case of the existing microbial antimicrobial activity test, a minimum inhibitory concentration test (MIC test) is performed. This is a result of diluting the antimicrobial activity sample by concentration in the microbial culture medium to prevent the bacteria from being inhibited at the minimum concentration. It is often different from the verification method or the actual product application results. So, to apply to each product, the bacteria are randomly contaminated in the formula of the basic product, and then a certain concentration of candidate preservatives is added to measure the inhibition of the bacteria. This is called a challenge test. Antimicrobial and antiseptic candidate substances that result in low concentrations at the minimum inhibitory concentration do not spread evenly when applied to the actual product, but are aggregated into fine water droplets, resulting in a phenomenon in which most of the efficacy is inferior.Therefore, more than the MIC test results. In most cases, you have to use sheep.

Therefore, it is necessary to develop antimicrobial and antiseptic substances that can replace existing antibacterial and antiseptic substances, which are not only strong and persistent, but also harmless to the human body or have very low toxicity, and do not harm even when used for a long time. Has become.

On the other hand, EWG (Environmental Working Group) is the most influential non-profit environmental organization in the U.S., and analyzed research data related to raw materials, as well as cosmetics and personal care products, and classified and evaluated the safety of research results on hazards from 1 to 10 grades. . Hazard criteria are classified as low hazard (green grade) for grades 1 to 2, moderate hazard for grades 3 to 6, and high hazard for grades 7 to 10, and EWG green with high safety. Grade refers to grades 1 to 2, which can be judged as safe ingredients.

KR 10-2001-0020208 A

In order to solve the problems of the existing preservatives as described above, it is intended to provide an antimicrobial composition that is safe and non-irritating, and has a wide range of antibacterial and antiseptic properties against bacteria and fungi by using raw materials of EWG grade 1 harmless to the human body.

Therefore, it provides an antibacterial composition comprising ethylhexyl glycerin, caprylyl glycol, methylpropanediol, and a surfactant.

In addition, it provides a cosmetic composition comprising the antibacterial composition.

One aspect provides an antimicrobial composition comprising ethylhexyl glycerin, caprylyl glycol, methylpropanediol, and a surfactant.

The "antibacterial composition" may mean a composition having an antibacterial effect. In the present specification, the "antibacterial composition" may be used interchangeably with "antibacterial agent", "preservative" or "preservative".

"Antibacterial" means resistance to all contaminated microorganisms such as bacteria, fungi, yeast, etc., "antibacterial power" means defense power against these contaminating microorganisms, and "antiseptic power" refers to decay by contamination by microorganisms. It means defense against, and it is a concept that includes antibacterial power. "Preservative" means a substance that has antiseptic or antioxidant power and can prevent deterioration of a product for a long time and maintain its original state, and "preservation power" means a defense that can prevent deterioration of a product for a long time and maintain its original state. it means.

The antimicrobial composition includes a combination of ethylhexyl glycerin, caprylyl glycol, methylpropanediol, and a surfactant, and thus may have a synergistic antibacterial effect compared to using each component alone. Therefore, even when added to a cosmetic composition or a pharmaceutical composition for external application for skin using a minimum amount, excellent antibacterial effects can be exhibited. Since the antibacterial composition exhibits excellent antibacterial activity even in a small amount, it does not affect the formulation and can be reduced in cost.

The antimicrobial composition includes a combination of ethylhexyl glycerin, caprylyl glycol, methylpropanediol, and a surfactant, and thus can have broad antimicrobial activity against both bacteria and fungi. Specifically, the bacteria or fungi are microorganisms of the genus Escherichia , including Escherichia coli , and the like; Microorganisms of the genus Pseudomonas including Pseudomonas aeruginosa and the like; Enterovector aerogenes ( Enterobacter aerogenes ), including microorganisms in the genus Enterovector; Microorganisms of the genus Bacillus including Bacillus cereus and the like; Microorganisms of the genus Staphylococcus including Staphylococcus aureus and the like; Microorganisms of the genus Saccharomyces including Saccharomyces cerevisiae and the like; Microorganisms of the genus Candadia including Candida albicans and the like; Microorganisms of the genus Aspergillus , including Aspergillus niger , may be included, but are not limited thereto.

Ethylhexyl glycerin, caprylyl glycol, methylpropanediol and surfactant contained in the antimicrobial composition all correspond to the first and second grades of EWG's green grades. Therefore, the antimicrobial composition can be very excellent in safety.

"Ethylhexylglycerin" means "Octoxyglycerin" or "3-[(2-ethylhexyl)oxy]-1,2-propanediol (3-[(2-Ethylhexyl)oxy]-1 ,2-propanediol)", it is also a type of glycerin. The ethylhexyl glycerin may increase the antibacterial activity of the antibacterial composition, and may exhibit a deodorizing effect by inhibiting the growth of anaerobic bacteria. In addition, ethylhexyl glycerin can provide vitality to the skin by serving as skin conditioning, and can serve as a solvent to dissolve other components.

The ethylhexyl glycerin is 10 to 30% by weight, 10 to 25% by weight, 10 to 23% by weight, 10 to 21% by weight, 15 to 30% by weight, 15 to 25% by weight, 15 to 23% by weight based on the total weight of the antibacterial composition. Wt%, 15 to 21 wt%, 17 to 30 wt%, 17 to 25 wt%, 17 to 23 wt%, 17 to 21 wt%, 19 to 30 wt%, 19 to 25 wt%, 19 to 23 wt% , Or 19 to 21% by weight may be included. The ethylhexyl glycerin may be included in, for example, 20% by weight based on the total weight of the antibacterial composition, but is not limited thereto.

"Caprylyl glycol" is also referred to as "1,2-octanediol", and is a component extracted from coconut oil. The caprylyl glycol is a skin conditioning agent having antibacterial power and may serve as an antibacterial agent while softening the skin. Additionally, caprylyl glycol can help maintain moisture by regulating skin moisture.

The caprylyl glycol is 10 to 30% by weight, 10 to 25% by weight, 10 to 23% by weight, 10 to 21% by weight, 15 to 30% by weight, 15 to 25% by weight, 15 to 23 wt%, 15 to 21 wt%, 17 to 30 wt%, 17 to 25 wt%, 17 to 23 wt%, 17 to 21 wt%, 19 to 30 wt%, 19 to 25 wt%, 19 to 23 wt %, or 19 to 21% by weight. The caprylyl glycol may be included in, for example, 20% by weight based on the total weight of the antibacterial composition, but is not limited thereto.

"Methylpropanediol" is also called "2-methyl-1,3-propanediol" and is a type of glycol. The methylpropanediol is a colorless and odorless component and does not have irritation. The methylpropanediol has good compatibility and low minimum inhibitory concentration.

The methylpropanediol is 30 to 70% by weight, 30 to 60% by weight, 40 to 70% by weight, 40 to 60% by weight, 50 to 70% by weight, 50 to 65% by weight, 50 to 60% based on the total weight of the antibacterial composition. It may be included in wt%, 55 to 70 wt%, 55 to 65 wt%, or 55 to 60 wt%. The methylpropanediol may be included in, for example, 59% by weight based on the total weight of the antibacterial composition, but is not limited thereto.

The surfactant may be used without limiting its type, as long as it can stabilize the cosmetic formulation. In one embodiment, the surfactant may be an EWG grade 1 surfactant. In another embodiment, the surfactant may be an HLB 14 or higher surfactant among EWG grade 1 surfactants. For example, the surfactant may be a steareth component, behenes component, polyglyceryl laurate, etc., but is not limited thereto. In one embodiment, the surfactant is Steareth-30, Steareth-25, Beheneth-30, Polyglyceryl-10-laurate (Polyglyceryl- 10-laurate), but is not limited thereto.

"Steareth-30" is prepared by reacting 30 moles of ethylene oxide with stearyl alcohol, and may have a wax formulation.

The content of the surfactant may be appropriately selected by a person skilled in the art in consideration of the stability of the formulation. Cosmetics are often in O/W (oil-in-water) formulation, and in this case, it should be well dispersed and it is preferable to use a small amount of surfactant. This is because if a large amount of surfactant is included, it may break the stability when applied to a product and affect the quality of the product. In one embodiment, the surfactant is 0.1 to 3% by weight, 0.1 to 2% by weight, 0.1 to 1% by weight, 0.5 to 3% by weight, 0.5 to 2% by weight, 0.5 to 1.5% by weight based on the total weight of the antibacterial composition , Or 0.5 to 1% by weight may be included. The surfactant may be included in, for example, 1% by weight based on the total weight of the antibacterial composition, but is not limited thereto.

The antibacterial composition may include 10 to 30% by weight of ethylhexyl glycerin, 10 to 30% by weight of caprylyl glycol, 30 to 70% by weight of methylpropanediol, and 0.1 to 3% by weight of a surfactant based on the total weight of the antibacterial composition. .

The antimicrobial composition may be composed of 10 to 30% by weight of ethylhexyl glycerin, 10 to 30% by weight of caprylyl glycol, 30 to 70% by weight of methylpropanediol, and 0.1 to 3% by weight of a surfactant based on the total weight of the antimicrobial composition. .

The antimicrobial composition comprises ethylhexyl glycerin, caprylyl glycol, methylpropanediol, and surfactant in a weight ratio of 10 to 30: 10 to 30: 30 to 70: 0.1 to 3, or 15 to 25: 15 to 25: 50 to 60 : It can be included in a weight ratio of 0.1 to 2.

The antibacterial composition may be a transparent, colorless and odorless composition. The conventional preservatives 2-phenoxyethanol and 1,2-hexanediol have a characteristic odor, which affects the product and reduces the feeling of use, whereas the antimicrobial composition according to the above aspect is colorless and odorless and does not affect the product. Can be used.

The antimicrobial composition is easy to apply to the formulation and may not be separated. Therefore, when the antibacterial composition is used as a preservative included in a cosmetic composition or a pharmaceutical composition for external application for skin, it can exhibit high antibacterial efficacy in a stable formulation.

The antibacterial composition also showed excellent efficacy in a challenge test. That is, it was confirmed that the antimicrobial composition of the present invention has excellent antimicrobial activity in the results based on a challenge test with high reliability rather than a minimum inhibitory concentration test with poor effectiveness.

According to another aspect of the present invention, there is provided a cosmetic composition comprising the antibacterial composition.

The cosmetic composition may be provided as a cosmetic composition having high preservation power and safety by including the antibacterial composition. The content of the antibacterial composition used in the cosmetic composition is not particularly limited as long as it can secure the desired preservation power, and can be appropriately determined by those skilled in the art according to the degree of desired preservation power. For example, the antibacterial composition may be included in 0.01 to 10% by weight, 0.01 to 5% by weight, 0.01 to 2% by weight, or 0.01 to 1% by weight based on the total weight of the cosmetic composition. However, since the antibacterial composition exhibits excellent synergistic antibacterial effect even at a low content, it may exhibit a sufficient antibacterial effect even if it is used in an amount of 1% by weight or less based on the total weight of the cosmetic composition.

The cosmetic composition may be any formulation known as a cosmetic composition, for example, skin, emulsion, cream, sun cream, foundation, essence, gel, pack, mask pack, foam cleanser, body cleanser, softening lotion, eyeliner, It may be one formulation selected from the group consisting of shampoo, conditioner, soap, hairdressing agent, wool agent, hair cream, hair styling gel, lubricant, toothpaste, and wet tissue, but is not limited thereto.

Another aspect provides a pharmaceutical composition for external application for skin comprising the antibacterial composition.

The pharmaceutical composition for external application for skin may be provided as a pharmaceutical composition that has high preservation power and safety by including the antibacterial composition and can be applied as an external preparation for skin.

The term "skin external preparation" is a concept encompassing the entire composition applied to the skin, and includes, for example, various cosmetics such as basic cosmetics, makeup cosmetics, and hair cosmetics; It is a concept including pharmaceutical compositions such as ointments, creams, lotions, etc. applied for external use of the skin including pharmaceuticals and quasi-drugs.

The content of the antimicrobial composition used in the pharmaceutical composition for external application for skin is not particularly limited as long as it can secure a desired preservation power, and can be appropriately determined by a person skilled in the art according to the degree of desired preservation power. For example, the antibacterial composition may be included in 0.01 to 10% by weight, 0.01 to 5% by weight, 0.01 to 2% by weight, or 0.01 to 1% by weight based on the total weight of the pharmaceutical composition for external application for skin. However, since the antibacterial composition exhibits excellent synergistic antibacterial effect even at a low content, it may exhibit sufficient antibacterial effect even if it is used in an amount of 1% by weight or less based on the total weight of the pharmaceutical composition for external application for skin.

The pharmaceutical composition for external application for skin may be any formulation known as an external preparation for skin, for example, a warning agent, a liniment agent, an ointment, a pasta agent, a cataplasma agent, a suspension agent, an emulsion, a lotion agent, an aerosol agent, and a suppository. It may be one formulation selected from the group consisting of, but is not limited thereto.

The pharmaceutical composition for external application for skin may be for the systemic action of the active ingredient by skin application, or may be for the local action of the active ingredient by skin application. In one embodiment, the pharmaceutical composition is for topical action of an active ingredient by skin application.

In addition to the antibacterial composition, the cosmetic composition and the pharmaceutical composition for external application for skin may further include various known additives within a range that does not impair the effect of the antibacterial composition according to the formulation, and according to one embodiment, a carrier, an emulsifier , A moisturizing agent, a skin conditioning agent, a chelating agent, an antioxidant, a disinfectant, a stabilizer, and an additive selected from the group consisting of any combination thereof may be further included.

As the carrier, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, lactose, silica, aluminum hydroxide, calcium silicate, poly Amide powder, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, liquid diluent, ethoxylated isostearyl alcohol, Suspension agents such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, ise Thionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol Fatty acid esters and the like may be included, but are not limited thereto.

Examples of the emulsifier include liquid paraffin, cetyloctanoate, and stearic acid, but are not limited thereto.

Examples of the moisturizing agent include glycerin and glyceryl stearate, but are not limited thereto.

Examples of the skin conditioning agent include cyclomethicone and dimethicone, but are not limited thereto.

Examples of the chelating agent include sodium ethylenediaminetetraacetate (EDTA), α-hydroxy fatty acid, lactoferrin, α-hydroxy acid, citric acid, lactic acid, malic acid, bilirubin, biliverdin, and the like, but are not limited thereto. .

Examples of the antioxidant include butylhydroxyanisole, dibutylhydroxytoluene, or propyl gallate, but are not limited thereto.

In addition, as components that can be blended into the cosmetic composition and pharmaceutical composition, there are oils and fats, emollients, organic and inorganic pigments, organic powders, ultraviolet absorbers, pH adjusters, alcohols, pigments, fragrances, blood circulation accelerators, cooling agents, and limiting agents. .

According to another aspect of the present invention, there is provided a method of preparing an external preparation for skin comprising using the antibacterial composition as a preservative.

For details of the method for preparing the external preparation for skin, the description of the antibacterial composition, the cosmetic composition, and the pharmaceutical composition for external preparation for skin according to an aspect of the present invention may be applied as it is.

In one embodiment, the external preparation for skin is a cosmetic composition or a pharmaceutical composition for external preparation for skin.

The preservative may be used in an amount of 0.01 to 10% by weight, 0.01 to 5% by weight, 0.01 to 2% by weight, or 0.01 to 1% by weight based on the total weight of the cosmetic composition or the pharmaceutical composition for external application for skin. However, since the antimicrobial composition exhibits excellent synergistic antibacterial effect even at a low content, it may exhibit sufficient antibacterial effect even if it is used in an amount of 1% by weight or less based on the total weight of a cosmetic composition or a pharmaceutical composition for external application for skin.

The antibacterial composition according to an aspect of the present invention is hypoallergenic and safe using only EWG grade 1 raw materials, and has a synergistic antibacterial effect than the efficacy of each component by using a combination of four components, and has a broad antimicrobial effect against microorganisms. Since it has a spectrum, it shows an excellent antibacterial effect even at a low concentration. Therefore, a cosmetic composition having high preservation power and safety can be prepared by using the antibacterial composition.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Example 1, Comparative Example 1, Comparative Example 2: Preparation of antibacterial composition

Antibacterial compositions of Example 1, Comparative Example 1, and Comparative Example 2 were prepared according to the components and contents shown in Table 1 below.

Raw material name
(Unit: wt%) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Ethylhexyl glycerin 20 100 0 0 Caprylyl glycol 20 0 100 0 Methylpropanediol 59 0 0 100 Steareth-30 One 0 0 0 Sum 100 100 100 100

Experimental Example 1: Antibacterial activity test in lotion formulation (Challenge Test)

The antimicrobial activity of the antimicrobial compositions of Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3 was tested against a total of five bacteria and fungi described in Table 2 below. All microorganisms used in the test are standard strains.

Microorganism name ATCC no. Escherichia coli 8739 Peudomonas aeruginosa 15692 Staphylococcus aureus 6538 Candida albicans 10231 Aspergillus niger 16404

In order to proceed with each experiment, the basic formulation 1 (lotion formulation) was prepared in the composition shown in Table 3 below. In order to promote the growth of microorganisms, the viscosity was lowered and cultured with stirring was performed to check the number of viable cells.

Basic Formulation 1 (Lotion Formulation) Content (w/w) Ethylhexyl stearate 6.5 Stearic acid 2.0 Cetearyl glucoside 1.0 glycerin 3.5 Carbomer 0.1 Tocopheryl acetate 3.0 water 83.9 Sodium hydroxide (up to pH 6.0) gs Total 100.0

First, after preparing samples of various concentrations (16-0.125%) by diluting each test antibacterial composition of Table 1 by 1/2, the microorganisms of Table 2 were 1×10 ⁵ -10 ⁶ CFU/ml concentration. The culture was adjusted so that each was added to 50 ml of the basic lotion formulation shown in Table 3.

Of the tested microorganisms, three kinds of bacteria ( E. coli , P. aeruginosa , S. aureus ) were cultured at 37°C for 8 days, and two fungi ( C. albicans , A. niger ) were cultured at 25°C for 8 days. Confirmation of growth, and the results are shown in Table 4 below. The decision was made on the basis of reaching a mortality rate of 95%.

Microorganism name Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 E. coli 0.5 1.0 1.0 >10 P. aeruginosa 0.25 4.0 1.0 >10 S. aureus 0.25 0.5 1.0 >10 C. albicans 0.25 0.5 1.0 >10 A. niger 0.5 1.0 1.0 >10

As can be seen in Table 4, compared to Comparative Example 1 using ethylhexyl glycerin alone, Comparative Example 2 using caprylyl glycol alone, and Comparative Example 3 using methylpropanediol alone, ethylhexyl glycerin and capryl Example 1 using a combination of ril glycol, methylpropanediol and surfactant was found to have a synergistic antibacterial effect even at a lower concentration of 0.5% (w/w) or less. In particular, in the case of Comparative Example 3, unlike the antibacterial effect was insignificant, the antibacterial composition of Example 1 showed a very excellent antibacterial effect. In addition, when the antimicrobial composition of Example 1 was used, the microbial death rate was 95% when cultured for 3 to 4 days.

Experimental Example 2: Antibacterial activity test in serum formulation (Challenge Test)

In order to improve the reliability of the experimental results, a basic formulation 2 (serum formulation) was prepared with the composition shown in Table 5 below, and a confirmation experiment was conducted.

Basic Formulation 2 (Serum Formulation) Content (w/w) Dimethicone 0.5 Cyclomethicone 1.0 Butylene glycol 6.0 Xanthan gum 0.4 Sodium PCA 1.5 Glyceres-26 2.0 Twin-20 0.1 water 88.5 Total 100.0

The antibacterial effect was confirmed using the same method as in Experimental Example 1, except that the basic formulation 2 (serum formulation) was used instead of the basic formulation 1 (lotion formulation) as the basic formulation, and the results are shown in Table 6. In addition, the judgment was made on the basis of reaching 95% mortality.

Microorganism name Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 E. coli 0.5 1.0 2.0 >10 P. aeruginosa 0.25 4.0 1.0 >10 S. aureus 0.25 0.5 1.0 >10 C. albicans 0.25 0.5 1.0 >10 A. niger 0.5 2.0 2.0 >10

The results in Table 6 were similar to Table 4, which is the result of Experimental Example 1. It was confirmed that the higher the water solubility, that is, the more the O/W (oil-in-water) formulation was, the more the antibacterial activity of Comparative Example 2 was lowered. It was confirmed that Comparative Example 2 was opaque and did not disperse in the formulation, but remained in the form of fine water droplets, thereby reducing the contact area with microorganisms, resulting in poor antibacterial activity.

Therefore, the antimicrobial composition according to an embodiment contains a combination of ethylhexyl glycerin, caprylyl glycol, and methylpropanediol, thereby exhibiting a synergistic antibacterial effect than when any one of them is used alone, and by including a surfactant It was found that the antimicrobial activity was further increased due to better dispersion in

Claims (7)

Consisting of 10 to 30% by weight of ethylhexyl glycerin, 10 to 30% by weight of caprylyl glycol, 30 to 70% by weight of methylpropanediol, and 0.1 to 3% by weight of a surfactant based on the total weight of the antimicrobial composition,
The surfactant is any one of steareth-30, steareth-25, behenes-30 and polyglyceryl-10-laurate,
Antimicrobial composition for use as a preservative in cosmetic compositions of O/W (oil-in-water) formulation. delete delete A cosmetic composition comprising the antibacterial composition according to claim 1. The cosmetic composition of claim 4, wherein the antibacterial composition is contained in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition. A method for producing a cosmetic composition comprising using the antibacterial composition according to claim 1 as a preservative. The method of claim 6, wherein the preservative is used in an amount of 0.01 to 10% by weight based on the total weight of the cosmetic composition.