KR102151573B1 - Herb vinegar and manufacturing method thereof - Google Patents
Herb vinegar and manufacturing method thereof Download PDFInfo
- Publication number
- KR102151573B1 KR102151573B1 KR1020180030898A KR20180030898A KR102151573B1 KR 102151573 B1 KR102151573 B1 KR 102151573B1 KR 1020180030898 A KR1020180030898 A KR 1020180030898A KR 20180030898 A KR20180030898 A KR 20180030898A KR 102151573 B1 KR102151573 B1 KR 102151573B1
- Authority
- KR
- South Korea
- Prior art keywords
- vinegar
- herbal
- powder
- acetic acid
- fermented
- Prior art date
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- 238000004519 manufacturing process Methods 0.000 title abstract description 23
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12J—VINEGAR; PREPARATION OR PURIFICATION THEREOF
- C12J1/00—Vinegar; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
- A23V2250/2132—Other phenolic compounds, polyphenols
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/02—Acetobacter
Abstract
본 발명은 허브 분말 또는 허브 농축액을 준비하는 단계; 상기 준비된 허브 분말 또는 허브 농축액을 고두밥, 누룩, 양파즙, 수곡 및 정제수와 혼합하여 발효하는 단계; 및 상기 발효를 통해 얻은 발효 주정에 종초를 첨가하여 초산 발효하는 단계;를 포함함으로써, 허브의 유효성분을 다량 함유하고 있으며, 폴리페놀 함량이 높을 뿐만 아니라 관능적으로도 우수한 허브식초 및 그 제조 방법에 관한 것이다.The present invention comprises the steps of preparing a herbal powder or herbal concentrate; Fermenting the prepared herbal powder or herbal concentrate with godubap, yeast, onion juice, grains, and purified water; And acetic acid fermentation by adding seed seeds to the fermented alcohol obtained through the fermentation; thereby containing a large amount of the active ingredient of the herb, and having a high polyphenol content and organoleptic excellent herbal vinegar and its manufacturing method. About.
Description
본 발명은 허브식초 및 그 제조방법에 관한 것이다.The present invention relates to herbal vinegar and its manufacturing method.
식초는 동·서양을 막론하고 오랜 옛날부터 제조, 이용되어온 발효식품의 하나이며, 일상생활에서 쉽게 사용하는 조미료이다. 식초는 보통 제조법에 따라 양조초와 합성초로 나뉜다. 양조초란 자연적으로 초산 발효 되어진 식초를 말하며, 합성초는 발효과정을 거치지 않고 빙초산 또는 초산을 희석하여 유기산을 첨가한 것으로 무색투명하다.Vinegar is one of the fermented foods that have been manufactured and used for a long time in both East and West, and is a seasoning that is easily used in everyday life. Vinegar is usually divided into brewed vinegar and synthetic vinegar according to the recipe. Brewed vinegar refers to vinegar that has been naturally fermented with acetic acid, and synthetic vinegar is colorless and transparent by diluting glacial acetic acid or acetic acid and adding organic acid without fermentation.
양조식초는 원료에 따라 여러 가지로 분류되는데 곡식을 원료로 하면 곡물초, 과실을 원료로 하면 과실초라고 한다. 만드는 방법에 따라 천연 양조식초와 알콜 양조식초로 나뉘는데 천연 양조식초는 알콜을 발효시켜 술을 만든 후 그것을 다시 초산 발효시켜 만드는 것이다. 알콜 양조식초는 순도 99% 이상의 에틸알콜을 원료로 하여 초산을 발효시킨 것이다. 자연 발효된 천연 양조식초에 알콜 주정을 혼합한 것을 혼합 양조식초라 한다. 하지만 몸에 가장 좋은 식초는 천연 양조식초, 즉 100%자연 발효된 양조식초이다.Brewed vinegar is classified into various types depending on the raw material, and it is called grain vinegar when grain is used as a raw material, and fruit vinegar when fruits are used as raw materials. According to the method of making, it is divided into natural brewed vinegar and alcohol brewed vinegar. Natural brewed vinegar is made by fermenting alcohol to make alcohol and then fermenting it with acetic acid. Alcohol brewed vinegar is fermented with acetic acid using ethyl alcohol of 99% or higher purity as a raw material. A mixture of naturally fermented natural brewed vinegar and alcoholic spirit is called mixed brewed vinegar. However, the best vinegar for the body is natural brewed vinegar, that is, 100% naturally fermented brewed vinegar.
이러한 식초는 함유된 유기산과 아미노산이 체내 에너지 대사에 관여해 피로물질이 쌓이는 것을 막아주며 동맥경화증이나 혈전증 등의 질병을 일으키는 과산화지질의 생성을 막아주는 역할을 한다. 또한 비만을 방지하고 피부 건강과 미용에도 좋을 뿐만아니라 인체 내 독성 제거 및 숙취 제거 등에도 효능이 있다.These vinegars are involved in energy metabolism in the body by the organic acids and amino acids contained therein, preventing the accumulation of fatigue substances, and preventing the formation of lipid peroxide that causes diseases such as atherosclerosis and thrombosis. In addition, it prevents obesity, is good for skin health and beauty, and is also effective in removing toxicity in the human body and removing hangovers.
그러나 현재 시중에 판매가 되고 있는 식초는 속성 알콜 양조식초로 천연식초가 필요로 하는 비타민과 유기산을 충분히 함유하고 있지 않기 때문에 그 효능이 떨어진다. 그래서 현재 건강에 좋은 다양한 재료들을 이용한 천연 식초들을 개발하려는 연구가 계속되고 있다.However, the vinegar that is currently sold on the market is a fast alcohol brewed vinegar and does not contain enough vitamins and organic acids that natural vinegar requires, so its efficacy is inferior. So, research is being conducted to develop natural vinegars using various ingredients that are good for health.
한편, 허브는 꽃과 종자, 줄기, 잎, 뿌리 등이 약, 요리, 향료, 살균, 살충 등에 사용되는 인간에게 유용한 초본식물로서 질병을 치료하거나 예방하는 효과가 있을 뿐만 아니라, 식품과 요리의 맛과 향은 물론 영양을 더하기 위해 두루 사용되는 오늘날 많은 관심과 주목을 받고 있는 천연소재로서 이를 이용하여 더욱 다양한 제품을 개발할 필요성이 대두 되고 있는 실정이다.On the other hand, herbs are useful herbal plants for humans whose flowers, seeds, stems, leaves, and roots are used for medicine, cooking, flavoring, sterilization, and insecticide, and have the effect of curing or preventing diseases, as well as the taste of food and cooking. As a natural material that is receiving a lot of attention and attention today, which is used not only for fruit and fragrance, but also for nutrition, there is a need to develop more diverse products using it.
예를 들면, 한국등록특허 제0923410호에서와 같이 최근 허브식초 제조방법과 그 허브식초가 개발되고 있으나, 상술한 바와 허브의 유용성을 갖는 허브식초 및 그 제조방법의 요구가 지속되고 있다.For example, as in Korean Patent Registration No. 0923410, a method for manufacturing herbal vinegar and its herbal vinegar have been recently developed, but as described above, the demand for herbal vinegar and a method for manufacturing the herbal vinegar having utility as described above continues.
본 발명의 일 과제는 허브의 유효성분을 다량 함유하고 있으며, 폴리페놀 함량이 높아 활성산소 흡수능력 및 항산화 능력이 우수한 허브식초 및 그 제조방법을 제공하는 것이다.An object of the present invention is to provide a herbal vinegar and a method of manufacturing the same, which contains a large amount of active ingredients of herbs, and has high active oxygen absorption capacity and antioxidant capacity due to high polyphenol content.
1. 허브 분말 또는 허브 농축액을 준비하는 단계; 상기 준비된 허브 분말 또는 허브 농축액을 고두밥, 누룩, 양파즙, 수곡 및 정제수와 혼합하여 발효하는 단계; 및 상기 발효를 통해 얻은 발효 주정에 종초를 첨가하여 초산 발효하는 단계;를 포함하는 허브식초 제조 방법.1. Preparing herbal powder or herbal concentrate; Fermenting the prepared herbal powder or herbal concentrate with godubap, yeast, onion juice, grains, and purified water; And acetic acid fermentation by adding seed to the fermented alcohol obtained through the fermentation.
2. 위 1에 있어서, 상기 초산 발효단계를 통해 얻은 초산 발효물에 허브 분말 또는 허브 농축액을 초산 발효물 기준으로 2 내지 7중량부 첨가하는 단계를 더 포함하는 허브식초 제조 방법.2. The method for manufacturing herbal vinegar according to the above 1, further comprising adding 2 to 7 parts by weight of the herb powder or the herb concentrate to the acetic acid fermented product obtained through the acetic acid fermentation step based on the acetic acid fermentation product.
3. 위 1에 있어서, 상기 허브 분말 또는 허브 농축액은 페퍼민트 및 레몬그라스 중 적어도 하나를 포함하여 제조되는 것인, 허브식초 제조 방법.3. The method of 1 above, wherein the herbal powder or herbal concentrate is prepared including at least one of peppermint and lemongrass.
4. 위 1에 있어서, 상기 허브 농축액은 상기 허브 분말을 열수 추출하고 농축시켜 얻는 것인, 허브식초 제조 방법.4. The method of 1 above, wherein the herbal concentrate is obtained by extracting and concentrating the herbal powder with hot water.
5. 위 1에 있어서, 상기 알코올 발효 단계는 허브 분말 또는 허브 농축액 0.15 내지 1.5 중량% 고두밥 18 내지 30 중량%, 수곡 0.2 내지 3 중량%, 누룩 0.4 내지 7.5 중량%, 양파즙 0.1 내지 0.5 중량% 및 정제수 60 내지 80 중량%를 혼합하여 발효시키는 것인, 허브식초 제조 방법.5. In the above 1, the alcohol fermentation step is herb powder or herb concentrate 0.15 to 1.5% by weight godubap 18 to 30% by weight, grain 0.2 to 3% by weight, yeast 0.4 to 7.5% by weight, onion juice 0.1 to 0.5% by weight And to ferment by mixing 60 to 80% by weight of purified water, herbal vinegar production method.
6. 위 5에 있어서, 상기 고두밥은 23 내지 26 중량%인, 허브식초 제조 방법.6. In the above 5, wherein the godubap is 23 to 26% by weight, herbal vinegar manufacturing method.
7. 위 5에 있어서, 상기 누룩은 4.5 내지 6 중량%인, 허브식초 제조 방법.7. In the above 5, wherein the yeast is 4.5 to 6% by weight, herbal vinegar manufacturing method.
8. 위 1에 있어서, 상기 종초는 초산균 RIC-V를 상기 발효 주정 중 일부에 접종하여 발효시킨 것인, 허브식초 제조 방법.8. The method of 1 above, wherein the seed is fermented by inoculating acetic acid bacteria RIC-V into some of the fermented alcoholic beverages.
9. 위 1 내지 8 중 어느 한 항의 방법에 의해 제조된 허브식초.9. Herbal vinegar prepared by the method of any one of the above 1 to 8.
본 발명의 실시예들에 따른 허브식초 및 그 제조방법은 허브의 유효성분을 다량 함유하고 있으며, 폴리페놀 함량이 높을 뿐만 아니라 관능적으로도 우수하다.Herbal vinegar and its manufacturing method according to embodiments of the present invention contain a large amount of the active ingredient of the herb, and the polyphenol content is high, and it is also excellent organoleptic.
도 1은 본 발명의 실시예들에 따른 허브식초 제조 방법을 개략적으로 나타내는 흐름도이다.1 is a flowchart schematically illustrating a method of manufacturing herbal vinegar according to embodiments of the present invention.
본 발명의 예시적인 실시예들은 허브 분말 또는 허브 농축액을 준비하는 단계; 상기 준비된 허브 분말 또는 허브 농축액을 고두밥, 누룩, 양파즙, 수곡 및 정제수와 혼합하여 발효하는 단계; 및 상기 발효를 통해 얻은 발효 주정에 종초를 첨가하여 초산 발효하는 단계; 를 포함함으로써, 허브의 유효성분을 다량 함유하고 있으며, 폴리페놀 함량이 높을 뿐만 아니라 관능적으로 우수한 허브 식초 및 그 제조방법을 제공할 수 있다.Exemplary embodiments of the present invention include preparing a herbal powder or herbal concentrate; Fermenting the prepared herbal powder or herbal concentrate with godubap, yeast, onion juice, grains, and purified water; And acetic acid fermentation by adding seed seeds to the fermented alcohol obtained through the fermentation. By including, it is possible to provide a herbal vinegar and a method of manufacturing the same, which contains a large amount of the active ingredient of the herb, has a high polyphenol content, and is organoleptic.
도 1에는 본 발명의 일 구현예에 따른 허브식초 제조 방법이 개략적으로 도시되어 있다. 이하, 도면을 참조하여 본 발명의 일 구현예에 따른 허브식초의 제조 방법을 상세히 설명한다. 그러나 이는 예시에 불과하며 본 발명은 이에 제한되지 않는다.1 schematically shows a method of manufacturing herbal vinegar according to an embodiment of the present invention. Hereinafter, a method of manufacturing herbal vinegar according to an embodiment of the present invention will be described in detail with reference to the drawings. However, this is only an example and the present invention is not limited thereto.
본 발명의 일 구현예에 따른 허브식초 제조 방법은 도 1에 도시된 바와 같이, 허브 분말 또는 허브 농축액을 준비하는 단계를 포함한다.Herbal vinegar manufacturing method according to an embodiment of the present invention, as shown in Figure 1, includes the step of preparing a herbal powder or herbal concentrate.
허브(Herb)는 잎이나 줄기가 식용과 약용으로 쓰이거나 향과 향미로 이용되는 식물로서, 탄수화물, 무기질, 비타민, 아미노산 등을 함유하여 면역기능을 강화하고, 소화제, 강장제, 소염제, 항암제로도 이용 가능하여 인간에게 유용한 식물로 널리 알려져 있다.Herb is a plant whose leaves and stems are used for edible and medicinal purposes, or as aroma and flavor. It contains carbohydrates, minerals, vitamins, amino acids, etc. to strengthen immune function, and also as a digestive, tonic, anti-inflammatory, and anticancer agent. It is widely known as a plant useful to humans because it is available.
일부 실시예에 있어서, 상기 허브는 정신적 피로와 우울증, 신경성 발작 등에 효과가 있고, 더울 때에는 차갑게 해주고 추울 때에는 따뜻하게 해주는 성분이 점액의 유출을 막아주고 해열과 발한을 도울 뿐만 아니라, 감기, 천식, 기관지염, 콜레라, 폐렴, 폐결핵, 식중독, 신경통 등에 효과가 있는 것으로 알려진 페퍼민트(Peppermint); 및 니아신, 비타민 A, 베타카로틴, 단박질, 철분, 칼슘, 칼륨, 비타민 B, 비타민 C등의 영양성분을 함유하며 복통, 설사, 두통, 발열 및 살균작용이 있는 것으로 알려진 레몬그라스(Lemongrass) 중 적어도 하나를 포함할 수 있다.In some embodiments, the herb is effective for mental fatigue, depression, and neurotic seizures, and ingredients that keep it cold when it is hot and warm when it is cold prevent the outflow of mucus and help fever and sweating, as well as colds, asthma, and bronchitis. , Cholera, pneumonia, pulmonary tuberculosis, food poisoning, and peppermint known to be effective in neuralgia, etc.; And niacin, vitamin A, beta-carotene, sweetness, iron, calcium, potassium, vitamin B, vitamin C, and other nutrients, and is known to have abdominal pain, diarrhea, headache, fever, and sterilization. It may include at least one.
그 밖에도 상기 허브의 비제한적인 예로서, 로즈마리, 장미허브, 라벤터, 캐모마일, 재스민, 시계초, 보리지, 크라리세이지, 아니카, 스파티움, 스위트베이, 타임 등을 들 수 있다.In addition, non-limiting examples of the herbs include rosemary, rose herb, lavender, chamomile, jasmine, passiflora, borage, craisage, anika, spatium, sweet bay, thyme, and the like.
본 발명에서는 상기 유용한 성분을 다량 함유한 허브를 세척, 절단, 건조 및 분쇄 과정을 통해 분말화하여 사용한다.In the present invention, a herb containing a large amount of the useful ingredients is powdered and used through washing, cutting, drying and grinding processes.
세척과정은 원료 유래의 물리적인 이물을 제거하고, 생물학적·화학적 위해요소를 식품분야에서 요구하는 허용수준까지 감소시키기 위해 수행되는 것으로, 예를 들면 허브 원료를 흐르는 물에 세척하여 수행될 수 있다.The washing process is performed to remove physical foreign matters derived from raw materials and reduce biological and chemical hazards to an acceptable level required in the food field, and may be performed, for example, by washing the herbal raw materials under running water.
절단과정은 열풍에 반응하는 허브 원료의 표면적을 넓혀서 건조 효율을 상승시키기 위해 수행되는 것으로, 상기 세척된 허브 원료를 2 내지 3㎝의 크기로 절단한다.The cutting process is performed to increase the drying efficiency by increasing the surface area of the herbal raw material reacting to the hot air, and the washed herbal raw material is cut into a size of 2 to 3 cm.
건조과정은 허브 원료의 수분 활성도를 낮춰서 미생물의 대사를 억제하고 저장성을 좋게 하기 위하여 건조하는 것으로, 예를 들면 열풍건조기를 사용하여 허브 원료를 건조할 수 있다.The drying process is to reduce the water activity of the herbal raw material to suppress the metabolism of microorganisms and to improve the storage properties. For example, the herbal raw material can be dried using a hot air dryer.
분쇄과정은 물리적인 확산과 침투, 용출, 에너지전달 등의 효과를 상승시키고, 화학적인 반응 효율을 높이기 위해 반응면적을 넓힐 수 있도록 상기 건조과정을 통해 건조된 허브 원료를 분쇄하여 분말화하는 과정이다.The pulverization process is a process of pulverizing and pulverizing the dried herb raw material through the above drying process to increase the effect of physical diffusion, penetration, elution, and energy transfer, and to increase the reaction area to increase chemical reaction efficiency. .
또한, 본 발명에서는 상기 과정들을 통해 제조된 허브 분말을 열수추출하고 농축하여 제조된 허브 농축액을 사용할 수 있다.In addition, in the present invention, a herbal concentrate prepared by extracting and concentrating the herbal powder prepared through the above processes with hot water may be used.
상기 열수추출은 당분야에서 통상적으로 사용되는 방법일 수 있으며, 예를 들면 허브 분말을 열수를 용매로 허브의 수용성 성분을 용출하고 남은 고형분을 여과하여 얻은 수용액과 증류된 수용액을 섞는 방법을 통해 이루어질 수 있다.The hot water extraction may be a method commonly used in the art, for example, by mixing the aqueous solution obtained by eluting the water-soluble component of the herb using hot water as a solvent and filtering the remaining solid content with the distilled aqueous solution. I can.
다음으로, 열수추출물을 밀폐 환경에서 대기압 이하로 감압하여 물을 끓는 점을 낮춰서 증발하여 농축함으로써 허브 농축액을 제조할 수 있다.Next, the hot water extract can be reduced to below atmospheric pressure in a sealed environment to lower the boiling point of water and evaporate to concentrate, thereby preparing a herbal concentrate.
상기 과정들을 통해 제조된 허브 분말 및 허브 농축액은 필요에 따라 공기 중의 수분과 산소, 미생물, 곤충, 이물 등 외부유래의 오염과 반응에 의한 화학적 변화, 물리적인 변화를 막기 위해 밀봉하고 포장하여 보관할 수 있으며, 밀봉 포장된 허브 분말 또는 허브 농축액을 본 발명에 의한 허브 식초 제조시에 사용할 수 있다.The herbal powder and herbal concentrate prepared through the above processes can be sealed and packaged and stored as necessary to prevent chemical and physical changes due to external contamination and reactions such as moisture and oxygen in the air, microorganisms, insects, and foreign matter. In addition, the hermetically packaged herbal powder or herbal concentrate can be used in the preparation of herbal vinegar according to the present invention.
허브 분말 및 허브 농축액은 그 함량이 특별히 제한되는 것은 아니나, 후술할 발효 단계에서 생성되는 알코올 발효물의 총 중량을 기준으로 0.15 내지 1.5 중량% 포함될 수 있다.The content of the herbal powder and the herbal concentrate is not particularly limited, but may be included in an amount of 0.15 to 1.5% by weight based on the total weight of the fermented alcohol produced in the fermentation step to be described later.
상기 허브 분말 및 허브 농축액은 상기 함량범위에서 허브의 효능을 충분히 나타낼 수 있다.The herbal powder and herbal concentrate may sufficiently exhibit the efficacy of the herb in the above content range.
본 발명의 일 구현예에 따르면, 상기 허브 분말 또는 허브 농축액을 고두밥, 누룩, 양파즙, 수곡 및 정제수와 혼합하여 발효하는 단계를 포함한다.According to one embodiment of the present invention, it includes the step of fermenting the herb powder or herbal concentrate mixed with godubap, yeast, onion juice, grains, and purified water.
일부 실시예들에 있어서, 상기 혼합 전 상기 고두밥, 누룩, 양파즙 및 수곡의 불가식 부위를 제거하는 정선 단계를 포함할 수 있으며, 흐르는 물로 이물질을 제거하는 세척 단계를 포함할 수 있다.In some embodiments, prior to mixing, the mixing may include a selecting step of removing the inedible portions of the godubap, yeast, onion juice, and grain, and a washing step of removing foreign substances with running water.
예시적인 실시예들에 있어서, 상기 혼합은 50 내지 300rpm의 속도로 교반하여 수행될 수 있다. 또한, 상기 혼합은 1 내지 5분에 걸쳐 수행될 수 있다.In exemplary embodiments, the mixing may be performed by stirring at a speed of 50 to 300 rpm. In addition, the mixing may be carried out over 1 to 5 minutes.
상기 발효는 미생물에 의해 당류가 알코올(예를 들면, 에탄올)과 이산화탄소로 분해되어 알코올을 생성하는 발효를 의미하며, 상기 알코올을 생성하는 발효단계를 통해 제조된 알코올 발효물은 에탄올 함량이 6 내지 9중량%인 것이 바람직하나 이에 한정되지 않는다.The fermentation refers to fermentation in which sugars are decomposed into alcohol (for example, ethanol) and carbon dioxide by microorganisms to produce alcohol, and the alcohol fermented product prepared through the fermentation step of generating alcohol has an ethanol content of 6 to It is preferably 9% by weight, but is not limited thereto.
알코올이 생성된 발효물의 에탄올 함량이 6중량% 미만이면 초산 발효단계에서 원하는 양의 초산을 얻지 못할 수 있으며, 에탄올 함량이 9중량%를 초과하면, 초산균의 생육에 불리한 영향을 주거나 일부 알코올이 초산으로 산화되지 못하고 식초내에 잔존할 우려가 있으므로, 에탄올 함량은 상기 범위로 형성되는 것이 바람직하다. 보다 바람직하게는, 초산발효 후의 초산 함량을 4중량% 이상으로 하기 위해 에탄올 함량을 6 내지 7중량%로 할 수 있다.If the ethanol content of the fermented product containing alcohol is less than 6% by weight, the desired amount of acetic acid may not be obtained in the acetic acid fermentation step.If the ethanol content exceeds 9% by weight, it adversely affects the growth of acetic acid bacteria or some alcohol Since it is not oxidized and may remain in the vinegar, the ethanol content is preferably formed within the above range. More preferably, the ethanol content may be 6 to 7% by weight in order to increase the acetic acid content after acetic acid fermentation to 4% by weight or more.
본 발명에서 고두밥은 통상적으로 식혜나 술을 만들 때 발효에 드는 시간을 줄이기 위해 사용하는 것으로서, 당업계에서 통상적으로 사용되는 방법을 통해 제조된 것 일 수 있으며, 예를 들어, 현미를 10 내지 24시간동안 침지한 후 수분을 제거하고 100℃의 증기로 60 내지 100분 동안 가열 후 30분간 뜸을 들여 제조된 것일 수 있다.In the present invention, godubap is typically used to reduce the time required for fermentation when making sikhye or sake, and may be prepared through a method commonly used in the art, for example, brown rice from 10 to 24 After immersing for a period of time, water may be removed, heated with steam at 100° C. for 60 to 100 minutes, and then steamed for 30 minutes.
고두밥은 알코올 발효물의 전체 중량을 기준으로 18 내지 30 중량% 포함될 수 있으며, 알코올 발효물의 에탄올 함량을 바람직한 범위인 6 내지 9중량%로 형성시키기 위한 측면에서 23 내지 26중량%로 포함될 수 있다.Godubap may be included in an amount of 18 to 30% by weight based on the total weight of the fermented alcoholic beverage, and may be included in an amount of 23 to 26% by weight in terms of forming the ethanol content of the fermented alcohol in a preferred range of 6 to 9% by weight.
누룩은 술을 만드는 효소를 갖는 곰팡이를 곡류에 번식시킨 것을 의미한다. Nuruk refers to a fungus that has an enzyme that makes alcoholic beverages grown on cereals.
상기 누룩은 본 발명에서 발효제로 사용되며 당업계에서 통상적으로 사용되는 것이 제한없이 사용될 수 있으나, 예를 들면, 통밀 및 물을 80:20의 중량비로 혼합하고, 혼합된 반죽을 면포로 감싸 누룩을 제조하기 위한 틀에 넣어 입자간 틈이 없을 만큼 다진 후, 틀에 넣어 성형된 누룩을 용기에 짚을 깔아 놓고 넣어 빛을 차단하여 25 내지 45℃에서 12 내지 18일간 누룩곰팡이를 배양시킨 것일 수 있다.The yeast is used as a fermenting agent in the present invention, and those commonly used in the art may be used without limitation, but for example, whole wheat and water are mixed in a weight ratio of 80:20, and the mixed dough is wrapped with a cotton cloth to form the yeast. After putting it in a mold for manufacturing and chopping it to the extent that there are no gaps between particles, the molded yeast put in the mold may have been cultivated for 12 to 18 days at 25 to 45°C by placing straw on the container and putting it to block light.
누룩은 알코올 발효물의 전체 중량을 기준으로 0.4 내지 7.5 중량% 포함될 수 있으며, 알코올 발효물의 에탄올 함량을 바람직한 범위인 6 내지 9%로 형성시키기 위한 측면에서 4.5 내지 6중량%로 포함될 수 있다.Nuruk may be included in an amount of 0.4 to 7.5% by weight based on the total weight of the fermented alcoholic product, and may be included in an amount of 4.5 to 6% by weight in order to form the ethanol content of the fermented alcoholic product in a preferred range of 6 to 9%.
본 발명에서 사용되는 수곡은 누룩을 미지근한 물에 담가 불린 것을 의미하며, 예를 들면, 누룩 및 물을 3:1의 중량비로 혼합하여 제조된 것일 수 있다.The grain used in the present invention means that yeast is soaked in lukewarm water, and may be prepared by mixing, for example, yeast and water in a weight ratio of 3:1.
본 발명에서 상기 수곡은 발효제로서 사용될 수 있으며, 알코올 발효물 전체 중량을 기준으로 0.2 내지 3중량% 포함될 수 있다. 함량이 0.2 중량% 미만일 경우 발효가 일어나지 않을 수 있으며, 3% 초과일 경우 발효가 과도하게 진행되거나, 제조된 식초에 수곡이 잔류하여 식초의 품질을 저하시킬 수 있다.In the present invention, the grain may be used as a fermenting agent, and may be contained in an amount of 0.2 to 3% by weight based on the total weight of the fermented alcohol. If the content is less than 0.2% by weight, fermentation may not occur, and if the content is more than 3%, fermentation proceeds excessively, or grains remain in the prepared vinegar, which may degrade the quality of vinegar.
본 발명에서 양파즙은 알코올 발효물의 전체 중량을 기준으로 0.1 내지 0.5 중량% 포함될 수 있으며, 알코올 발효물의 에탄올 함량이 바람직한 범위인 6 내지 9중량%로 형성되기 위한 측면에서 0.3 내지 0.5중량%로 포함될 수 있다.In the present invention, the onion juice may be contained in an amount of 0.1 to 0.5% by weight based on the total weight of the fermented alcoholic product, and the ethanol content of the fermented alcoholic product may be contained in an amount of 0.3 to 0.5% by weight in terms of forming a preferable range of 6 to 9% by weight. I can.
또한, 알코올을 생성하는 발효 단계는 허브분말 또는 허브농축액과, 고두밥, 누룩, 양파즙 및 수곡 외에 정제수를 함께 혼합한다.In addition, in the fermentation step of producing alcohol, the herbal powder or herbal concentrate, godubap, koji, onion juice, and grain, and purified water are mixed together.
상기 정제수는 알코올 발효물의 전체 중량을 기준으로 60 내지 80 중량% 포함될 수 있다. 정제수는 상기 범위로 포함되어 다른 물질들이 잘 혼합되도록 하면서 에탄올의 함량이 과도하게 높아지거나 낮아지는 것을 방지할 수 있다.The purified water may be included in an amount of 60 to 80% by weight based on the total weight of the fermented alcohol. Purified water is included in the above range so that other substances are well mixed, and the content of ethanol can be prevented from being excessively increased or decreased.
본 발명은 허브 분말 또는 허브 농축액과 고두밥, 누룩, 양파즙, 수곡 및 정제수를 혼합하여 산소를 차단하고 25℃ 이상에서 약 10일 발효함으로써, 혼합 발효물에 알코올을 생성시켜 알코올 발효물인 발효 주정을 얻을 수 있다.The present invention blocks oxygen by mixing herb powder or herbal concentrate with godubap, yeast, onion juice, grain, and purified water to block oxygen and ferment at 25°C or higher for about 10 days, thereby producing alcohol in the mixed fermented product to produce fermented alcoholic beverage. Can be obtained.
상기 발효 주정은 예를 들면, 상기 알코올 발효 과정에서 생성된 술지게미를 포함할 수 있다. 술지게미는 악취를 풍길 수 있으며, 누룩균을 포함할 수 있다.The fermented alcoholic beverage may include, for example, sake lees produced in the alcohol fermentation process. Sake lees may have a foul odor and may contain yeast.
일부 실시예에 있어서, 상기 발효 주정에서 술지게미를 제거할 수 있다. 술지게미를 제거함으로써 술지게미로 인한 악취를 제거할 수 있으며, 술지게미에 포함된 누룩균의 추가 발효에 의한 악취를 제거할 수 있다.In some embodiments, sake lees may be removed from the fermented alcoholic beverage. By removing the sake lees, the odor caused by the sake lees can be removed, and the odor caused by the additional fermentation of koji bacteria contained in the sake lees can be removed.
도 1을 참조하면, 본 발명의 일 구현예에 따른 허브식초 제조 방법은 상기 발효 주정에 종초를 첨가하여 초산 발효하는 단계를 포함한다.Referring to FIG. 1, a method of manufacturing herbal vinegar according to an embodiment of the present invention includes adding seed vinegar to the fermented alcohol to ferment with acetic acid.
본 발명의 허브 식초 제조방법에 있어서, 초산 발효는 알코올을 먹이로 초산균이 호기적 환경에서 증식하여 알코올을 산화시켜 초산, 즉 식초를 생성하는 단계이다. 예를 들면, 에탄올이 산소와 반응하여 아세트산과 물이 생성되는 반응을 통해 아세트산(초산)이 생성될 수 있다.In the herbal vinegar production method of the present invention, acetic acid fermentation is a step in which acetic acid bacteria grow in an aerobic environment by feeding alcohol to oxidize alcohol to produce acetic acid, that is, vinegar. For example, acetic acid (acetic acid) may be produced through a reaction in which ethanol reacts with oxygen to produce acetic acid and water.
본 발명에서 이용되는 종초는 초산균 RIC-V(A. sp. RIC-V, Acetobacter pomorum)으로 에탄올을 발효시켜 제조한 것일 수 있다. 또한, 상기 발효주정 중 일부에 초산균 RIC-V 접종하여 발효시킨 것 일 수 있다. 상기 종초의 산도는 4% 이상인 것이 바람직하며, 이에 제한되지 않는다.The seeds used in the present invention may be prepared by fermenting ethanol with acetic acid bacteria RIC-V (A. sp. RIC-V, Acetobacter pomorum). In addition, some of the fermented alcohol may be fermented by inoculating acetic acid bacteria RIC-V. The acidity of the seed is preferably 4% or more, but is not limited thereto.
일반적으로, 초산균 RIC-V를 에탄올 5중량%가 함유된 완전 멸균상태의 시료에 5중량% 접종한 후 약 30℃, 약 150rpm shaking incubator에서 발효시킬 경우 최고 산도에 도달하기까지 약 7일 내지 11일 정도가 소요될 수 있다. 또한 미숙과즙에 RIC-V를 접종하여 발효시킬 경우 초기산도 약 0.6%에서 최종산도 약 5.38%까지 발효될 수 있다.In general, when 5% by weight of acetic acid bacteria RIC-V is inoculated into a completely sterilized sample containing 5% by weight of ethanol and fermented in a shaking incubator at about 30°C and about 150 rpm, it takes about 7 to 11 days to reach the highest acidity. This may take up to a day. In addition, when RIC-V is inoculated and fermented in unripe fruit juice, it can ferment from about 0.6% initial acidity to about 5.38% final acidity.
일부 실시예들에 따르면, 상기 에탄올 발효에 의해 에탄올 약 5중량% 이상이 포함되고 초기산도가 약 0.6% 이상인 발효물을 제조할 수 있어, 상기 발효물에 RIC-V를 접종하여 발효 식초를 제조할 수 있다.According to some embodiments, a fermented product containing about 5% by weight or more of ethanol and having an initial acidity of about 0.6% or more may be prepared by the ethanol fermentation, and thus fermented vinegar is prepared by inoculating the fermented product with RIC-V. can do.
예시적인 실시예들에 있어서, 발효 주정이 허브 성분을 포함한다고 하더라도, RIC-V에 의해 초산 발효가 진행될 수 있다.In exemplary embodiments, even if the fermented alcoholic beverage contains an herbal component, acetic acid fermentation may be performed by RIC-V.
일부 실시예들에 있어서, 상기 종초는 발효 주정을 충분히 초산 발효시키기 위하여, 발효 주정 총 중량 대비 18 내지 23중량% 첨가될 수 있다.In some embodiments, the seed seeds may be added in an amount of 18 to 23% by weight based on the total weight of the fermented alcohol in order to sufficiently ferment the fermented alcohol with acetic acid.
일부 실시예들에 따르면, 상기 초산 발효는 상기 발효 주정에 상기 종초를 첨가한 후 약 30℃ 온도에서 약 14일간 수행될 수 있다. 이 경우, 발효 식초의 초산함량은 약 4중량% 이상일 수 있으나 이에 제한되지 않는다.According to some embodiments, the acetic acid fermentation may be performed for about 14 days at a temperature of about 30° C. after adding the seed seeds to the fermented alcohol. In this case, the acetic acid content of the fermented vinegar may be about 4% by weight or more, but is not limited thereto.
필요에 따라, 초산 발효단계를 통해 얻은 초산 발효물에 허브 분말 또는 허브 농축액을 초산 발효물 100 중량부를 기준으로 2 내지 7중량부 첨가하는 단계를 더 포함할 수 있다. 초산 발효물에 허브 분말 또는 허브 농축액을 상기 함량으로 첨가함으로써 폴리페놀의 함량을 증가시킬 수 있으며 산미를 증가시킬 수 있다.If necessary, it may further include the step of adding 2 to 7 parts by weight of the herbal powder or herbal concentrate to the acetic acid fermentation obtained through the acetic acid fermentation step based on 100 parts by weight of the acetic acid fermentation. The polyphenol content can be increased and acidity can be increased by adding the herbal powder or herbal concentrate to the acetic acid fermented product in the above amount.
허브 분말 또는 허브 농축액의 함량이 2중량부 미만일 경우, 폴리페놀의 함량과 산미의 증가가 미미하며, 7중량부를 초과할 경우, 제조된 식초에 허브 분말이 부유하거나 허브 성분의 향미가 과도하게 혼입되어 식초의 품질을 저하시킬 수 있다.If the content of herb powder or herbal concentrate is less than 2 parts by weight, the increase in the content of polyphenol and acidity is insignificant, and if it exceeds 7 parts by weight, the herb powder floats in the prepared vinegar or the flavor of the herb ingredient is excessively mixed. This can reduce the quality of vinegar.
일부 실시예들에 있어서, 상기 허브 농축액은 페퍼민트, 레몬그라스, 로즈마리, 스위트베이 및 타임으로부터 추출된 혼합 허브 농축액일 수 있다. 상기 혼합 허브 농축액은 페퍼민트 식초 및 레몬그라스 식초와 향미의 궁합이 뛰어나며, 페퍼민트 식초 및 레몬그라스 식초의 폴리페놀 함량을 향상시킬 수 있다.In some embodiments, the herbal concentrate may be a mixed herbal concentrate extracted from peppermint, lemongrass, rosemary, sweet bay, and thyme. The mixed herb concentrate has excellent compatibility between peppermint vinegar and lemongrass vinegar and flavor, and may improve the polyphenol content of peppermint vinegar and lemongrass vinegar.
필요에 따라, 본 발명에 의한 허브 식초 제조방법은 초산발효 완료 후, 0.9㎛이하의 메쉬를 갖는 여과기를 통해 고형물을 제거하고, 80℃ 이상에서 5분간 고온 살균하여 허브 식초 제조를 완료할 수 있다.If necessary, the herbal vinegar production method according to the present invention can complete the production of herbal vinegar by removing solids through a filter having a mesh of 0.9 μm or less after completion of acetic acid fermentation, and sterilizing at high temperature for 5 minutes at 80° C. or higher. .
본 발명의 예시적인 실시예에 따르면, 상기 허브 분말 또는 허브 농축액을 준비하는 단계, 상기 준비된 허브 분말 또는 허브 농축액을 고두밥, 누룩, 양파즙, 수곡 및 정제수와 혼합하여 발효하는 단계 및 상기 발효를 통해 얻은 발효 주정에 종초를 첨가하여 초산 발효하는 단계를 통해, 허브의 유효성분을 다량 함유하고 있으면서 폴리페놀 함량이 높을 뿐만 아니라 관능적으로도 우수한 허브 식초를 얻을 수 있다.According to an exemplary embodiment of the present invention, preparing the herbal powder or herbal concentrate, fermenting the prepared herbal powder or herbal concentrate with godubap, yeast, onion juice, grain and purified water, and fermenting through the fermentation. Through the step of acetic acid fermentation by adding seed vinegar to the obtained fermented alcohol, it is possible to obtain herbal vinegar that contains a large amount of the active ingredients of the herb, has a high polyphenol content, and is organoleptic.
이하, 본 발명의 이해를 돕기 위하여 바람직한 실시예를 제시하나, 이들 실시예는 본 발명을 예시하는 것일 뿐 첨부된 특허청구범위를 제한하는 것이 아니며, 본 발명의 범주 및 기술사상 범위 내에서 실시예에 대한 다양한 변경 및 수정이 가능함은 당업자에게 있어서 명백한 것이며, 이러한 변형 및 수정이 첨부된 특허청구범위에 속하는 것도 당연한 것이다.Hereinafter, preferred embodiments are presented to aid the understanding of the present invention, but these examples are only illustrative of the present invention and do not limit the scope of the appended claims, and examples within the scope and spirit of the present invention It is obvious to those skilled in the art that various changes and modifications are possible, and it is natural that such modifications and modifications fall within the scope of the appended claims.
실시예Example 1 내지 8 1 to 8
레몬그라스와 페퍼민트를 물로 세척하여 이물질을 제거하고, 3㎝의 크기로 절단한 후, 열풍건조기를 사용하여 건조하고, 분쇄기로 분쇄하여 레몬그라스 및 페퍼민트를 분말화하여 준비하였다.Lemongrass and peppermint were washed with water to remove foreign substances, cut into a size of 3 cm, dried using a hot air dryer, and pulverized with a grinder to pulverize lemongrass and peppermint.
발효 용기에 상기 준비된 레몬그라스 분말 또는 페퍼민트 분말과 고두밥, 누룩, 양파즙, 수곡 및 정제수를 표 1의 배합비대로 혼합하여 25℃의 항온실에서 15일 동안 알코올을 생성하는 발효를 진행하여 발효 주정을 얻었다.In a fermentation container, the prepared lemongrass powder or peppermint powder and godubap, yeast, onion juice, grain and purified water are mixed in the mixing ratio shown in Table 1, and fermentation is performed to generate alcohol for 15 days in a constant temperature room at 25°C. Got it.
상기 발효 주정에 종초를 발효 주정 중량 대비 20%를 첨가하여 30℃에서 14일 동안 초산발효를 진행하였다. 종초로는 알코올 농도 5.3%인 발효주정 200㎖에 초산균 RIC-V를 접종하여 진탕 배양기(shaking incubator)에서 30℃ 및 150rpm으로 배양하여 산도가 5%이상이 된 것을 사용하였다. 초산 발효가 완료된 후 초산 함량이 4% 이상인 허브식초가 완성되었으며, 이를 0.9㎛크기 메쉬를 갖는 여과기를 통해 고형물을 100℃에서 5분간 살균하여 허브 식초 제조를 완료하였다.The acetic acid fermentation was performed at 30° C. for 14 days by adding 20% of the fermented alcohol to the fermented alcohol based on the weight of the fermented alcohol. As a seed plant, RIC-V acetate was inoculated into 200 ml of fermented alcohol with an alcohol concentration of 5.3%, and cultured at 30°C and 150 rpm in a shaking incubator with an acidity of 5% or more. After the acetic acid fermentation was completed, herbal vinegar having an acetic acid content of 4% or more was completed, and the solids were sterilized at 100° C. for 5 minutes through a filter having a 0.9 μm size mesh to complete the production of herbal vinegar.
비교예Comparative example 1 및 2 1 and 2
레몬그라스 분말 또는 페퍼민트 분말을 사용하지 않는 것을 제외하고는 실시예와 동일하게 제조하였다.It was prepared in the same manner as in Examples, except that lemongrass powder or peppermint powder was not used.
(ml)Purified water
(ml)
(g)Godubap
(g)
(g)yeast
(g)
(누룩:물=3:1)Number of grains (g)
(Yeast: Water = 3: 1)
(g)Peppermint
(g)
(g)Lemongrass
(g)
(g)Onion juice
(g)
실시예Example 9 내지 20 9 to 20
하기 표 2에 따라 실시예 2, 5, 7 또는 8의 허브 식초에 페퍼민트 분말 또는 레몬그라스 분말을 일정 시간 침지하여 허브 분말 침지 허브 식초를 제조하였다.According to Table 2 below, peppermint powder or lemongrass powder was immersed in the herbal vinegar of Examples 2, 5, 7 or 8 for a certain period of time to prepare herbal powder immersed herbal vinegar.
실시예Example 21 내지 34 21 to 34
페퍼민트 분말 10g, 레몬그라스 분말 10g, 로즈마리 분말 55g, 스위트베이 분말 10g 및 타임 분말 15g을 포함하는 혼합 허브 분말을 제조하였다.A mixed herbal powder comprising 10 g of peppermint powder, 10 g of lemongrass powder, 55 g of rosemary powder, 10 g of sweet bay powder, and 15 g of thyme powder was prepared.
75℃ 온도의 열수 1000g을 이용해 상기 혼합 허브 분말의 수용성 성분을 용출하여 증류한 용액과 용출 후 남은 고형분을 여과하여 얻은 용액을 혼합하고, 혼합된 용액을 감압증류를 통해 100g으로 농축하여 혼합 허브 농축액을 제조하였다.The solution obtained by eluting the water-soluble component of the mixed herb powder with 1000 g of hot water at a temperature of 75° C. is mixed with the distilled solution and the solution obtained by filtration of the remaining solid after elution, and the mixed solution is concentrated to 100 g through reduced pressure distillation. Was prepared.
실시예 2, 5, 7 또는 8의 허브 식초에 하기 표 2의 중량% 해당하는 혼합 허브 농축액을 첨가하여 혼합 허브 농축액 첨가 허브 식초를 제조하였다.Herb vinegar with mixed herb concentrate was prepared by adding the mixed herb concentrate corresponding to the weight% of Table 2 to the herb vinegar of Examples 2, 5, 7 or 8.
실험예Experimental example
1. 폴리페놀 함량 분석1. Analysis of polyphenol content
실시예 및 비교예의 허브 식초 200μL와 증류수 1800μL을 혼합하고, Folin-Ciocalteau's phenol reagent 200μL을 가하여 잘 섞은 후 5분간 상온에서 반응시켰다. 그 후, 2M Na2CO3 400μL을 가하여 혼합한 다음 증류수를 가하여 4mL로 조정하였다. 이 용액을 상온 암소에서 1시간 동안 반응시킨 후 725nm에서 흡광도를 측정하였고, gallic acid(Sigma Chemical Co., St Louis, MO, USA)를 이용한 검량선과 비교하여 총 페놀함량(μg gallic acid equivalents(GAE)/mL)을 하기 표 3 및 표 4에 나타내었다.200 μL of herbal vinegar of Examples and Comparative Examples and 1800 μL of distilled water were mixed, 200 μL of Folin-Ciocalteau's phenol reagent was added, mixed well, and reacted at room temperature for 5 minutes. Then, 2M Na 2 CO 3 400 μL was added and mixed, and then distilled water was added to adjust to 4 mL. After reacting this solution for 1 hour in a dark place at room temperature, absorbance was measured at 725 nm, and the total phenol content (μg gallic acid equivalents (GAE) was compared with a calibration curve using gallic acid (Sigma Chemical Co., St Louis, MO, USA). )/mL) are shown in Tables 3 and 4 below.
2. 항산화 효과 평가2. Antioxidant effect evaluation
실시예 1 내지 8 및 비교예의 허브 식초의 항산화 효과를 ABST 자유유리기 소거 활성 실험을 통해 평가하였다.The antioxidant effects of herbal vinegars of Examples 1 to 8 and Comparative Examples were evaluated through ABST free free radical scavenging activity experiments.
시험 용액의 제조는 증류수에 7mM ABTS와 2.45mM K2S2O8(potassium persulfate)를 첨가하고, 상온에서 16시간 배양하여 ABTS 양이온(ABTS+)을 생성하였다. 그 후, 2.45mM K2S2O8 buffer와 동량 혼합하였을 때, 734nm에서의 흡광도의 값이 0.70(±0.02)이 되도록 희석하여 ABTS 시험 용액을 제조하였다.To prepare the test solution, 7mM ABTS and 2.45mM K 2 S 2 O 8 (potassium persulfate) were added to distilled water, and ABTS cation (ABTS + ) was generated by incubating for 16 hours at room temperature. Then, when the same amount was mixed with the 2.45 mM K 2 S 2 O 8 buffer, the ABTS test solution was prepared by diluting the absorbance value at 734 nm to 0.70 (±0.02).
실시예 및 비교예의 허브 식초에 ABTS 시험 용액을 동량 가하고 6분 후에 흡광도 값을 측정하였다. 대조군(2.45mM K2S2O8 buffer)의 흡광도와 비교하여 흡광도가 감소된 정도(%)를 하기 표 3에 나타내었다.The same amount of ABTS test solution was added to the herbal vinegar of Examples and Comparative Examples, and the absorbance value was measured 6 minutes later. Table 3 shows the degree (%) of reduced absorbance compared to the absorbance of the control group (2.45mM K 2 S 2 O 8 buffer).
상기 표 3을 참조하면, 폴리페놀의 함량이 증가함에 따라 ABTS 라디칼 소거 활성 값이 증가하는 것으로 나타나 폴리페놀의 함량과 식초의 항산화력이 양의 상관관계를 갖는 것을 알 수 있었다.Referring to Table 3, it was found that the ABTS radical scavenging activity value increased as the polyphenol content increased, indicating that the polyphenol content and the antioxidant power of vinegar had a positive correlation.
또한, 정제수 4L를 이용한 실시예 1 내지 6 및 비교예 1에 대하여, 페퍼민트 또는 레몬그라스 성분을 함유한 실시예 1 내지 6의 허브 식초의 폴리페놀 함량 및 ABTS 라티칼 소거 활성 값이 비교예 1의 식초보다 높게 나타났다. 또한, 정제수 2.5L를 이용한 실시예 7, 8 및 비교예 2에 대하여도 마찬가지로 페퍼민트 또는 레몬그라스 성분을 함유한 실시예 7 및 8의 허브 식초의 폴리페놀 함량 및 ABTS 라티칼 소거 활성 값이 비교예 2의 식초보다 높게 나타났다.In addition, with respect to Examples 1 to 6 and Comparative Example 1 using 4 L of purified water, the polyphenol content and ABTS radical scavenging activity values of the herbal vinegars of Examples 1 to 6 containing peppermint or lemongrass components were found in Comparative Example 1. It was higher than vinegar. In addition, for Examples 7, 8 and Comparative Example 2 using 2.5 L of purified water, the polyphenol content and ABTS radical scavenging activity values of the herbal vinegar of Examples 7 and 8 containing peppermint or lemongrass components were similarly compared. It was higher than that of vinegar in 2.
따라서, 허브 성분을 함유하여 제조된 식초가 폴리페놀 함량 및 항산화 효과가 증가하는 것을 알 수 있었다.Therefore, it was found that the polyphenol content and antioxidant effect of the vinegar prepared by containing the herbal component increased.
또한, 정제수 2.5L를 사용한 실시예 7 및 8의 경우 정제수 4L를 사용한 실시예 3 및 6에 비하여 폴리페놀 함량과 항산화력이 증가하는 것을 알 수 있었다.In addition, in the case of Examples 7 and 8 using 2.5 L of purified water, it was found that the polyphenol content and antioxidant power were increased compared to Examples 3 and 6 using 4 L of purified water.
상기 표 4를 참조하면, 추가적으로 허브 분말을 침지하여 제조한 실시예 9 내지 20의 허브 식초의 경우 그렇치 않은 실시예 2, 5, 7 및 8의 허브 식초에 비하여 폴리페놀을 다량 함유하였다. 또한, 허브 분말을 다량 침지할 경우 폴리페놀의 함량이 보다 증가하였다.Referring to Table 4, the herbal vinegar of Examples 9 to 20 prepared by immersing the herb powder additionally contained a large amount of polyphenol compared to the herbal vinegar of Examples 2, 5, 7 and 8, which was not so. In addition, when a large amount of herb powder was immersed, the content of polyphenols increased.
실시예 9 내지 16의 허브 식초에 대하여, 허브 분말을 1시간 침지한 경우(실시예 9, 11, 13 및 15)와, 4주간 침지한 경우(실시예 10, 12, 14 및 16)의 폴리페놀 함량이 크게 변화하지 않는 것으로 나타났다. 따라서, 허브 분말을 약 1시간 침지함으로써 허브 분말로부터 폴리페놀을 충분히 용출시킬 수 있음을 알 수 있었다.With respect to the herbal vinegar of Examples 9 to 16, the poly(Examples 9, 11, 13 and 15) and 4 weeks of soaking the herb powder for 1 hour (Examples 10, 12, 14 and 16) It was found that the phenol content did not change significantly. Therefore, it was found that polyphenols could be sufficiently eluted from the herb powder by immersing the herb powder for about 1 hour.
그리고, 허브 농축액을 첨가하여 제조한 실시예 21 내지 34의 허브 농축액 첨가 허브 식초의 경우 폴리페놀의 함량이 향상되는 것을 알 수 있었으며, 허브 농축액의 첨가량이 증가할수록 폴리페놀의 함량이 증가하는 것을 알 수 있었다.In addition, in the case of the herbal vinegar with the herbal concentrate of Examples 21 to 34 prepared by adding the herbal concentrate, it was found that the polyphenol content was improved, and the polyphenol content increased as the added amount of the herbal concentrate increased. Could
2. 기호도 관능 평가2. Sensory evaluation of preference
상기 실시예 및 비교예에 따라 제조한 허브 식초를 대상으로 하여 아래의 기호도 관능 평가를 실시하였다.Sensory evaluation of the following preference was also performed on the herbal vinegar prepared according to the above Examples and Comparative Examples.
기호도 관능 평가는 나이, 성별에 관계없이 임의로 모집된 40인의 시험단에 의해 수행되었다The preference and sensory evaluation was performed by a group of 40 randomly recruited regardless of age and sex.
1) 허브 무첨가 식초와 허브 식초의 기호도 평가1) Evaluation of preference of herbal vinegar and herbal vinegar
실시예 2, 5 및 비교예 1의 식초를 시험단이 시음하도록 한 후 종합적으로 우수하다고 느낀 식초에 투표하도록 하여 하기 표 5에 나타내었다.The vinegars of Examples 2 and 5 and Comparative Example 1 were sampled by the test group, and then voted for the vinegar that they felt as excellent overall, as shown in Table 5 below.
상기 표 5를 참조하면, 허브 성분을 함유하지 않는 비교예 1의 식초에 비하여 페퍼민트 또는 레몬그라스 성분을 포함하는 실시예 2 및 5의 허브 식초의 기호도가 보다 높게 나타났다. 따라서, 허브 식초가 허브 미함유 식초에 비하여 향미가 뛰어난 것을 알 수 있었다.Referring to Table 5, the preference of the herbal vinegar of Examples 2 and 5 containing peppermint or lemongrass components was higher than that of the vinegar of Comparative Example 1 not containing the herbal component. Therefore, it was found that herbal vinegar has superior flavor compared to vinegar containing no herbs.
2) 페퍼민트 분말 2) Peppermint powder 침지Immersion 페퍼민트 식초의 기호도 평가 Peppermint vinegar's preference rating
시험단에게 실시예 2, 10 및 12의 페퍼민트 식초를 시음하게 한 후 종합적으로 우수하다고 느낀 식초에 투표하도록 하여 하기 표 6에 나타내었다.After having the test group taste the peppermint vinegar of Examples 2, 10, and 12, they were asked to vote for the vinegar that they felt as excellent overall, and are shown in Table 6 below.
상기 표 6을 참조하면, 초산 발효 후 추가적으로 페퍼민트 분말을 침지한 실시예 10 및 12의 페퍼민트 식초가 추가적으로 페퍼민트 분말을 침지하지 않은 실시예 2의 페퍼민트 식초보다 기호도가 높게 나타났다. 따라서, 페퍼민트 분말을 추가로 침지하여 페퍼민트 성분이 추가적으로 함유된 페퍼민트 식초가 향미가 보다 뛰어난 것을 알 수 있었다. 또한, 식초 총 중량 대비 5중량%의 페퍼민트 분말을 침지한 실시예 12의 식초가 3중량%의 페퍼민트 분말을 침지한 실시예 10의 식초보다 우수한 향미를 갖는 것을 알 수 있었다.Referring to Table 6, the peppermint vinegar of Examples 10 and 12 additionally immersed in peppermint powder after acetic acid fermentation showed higher preference than the peppermint vinegar of Example 2 not additionally immersed in peppermint powder. Therefore, it was found that peppermint vinegar containing additional peppermint components by additionally immersing peppermint powder has superior flavor. In addition, it was found that the vinegar of Example 12 in which 5% by weight of peppermint powder was immersed relative to the total weight of the vinegar had a better flavor than the vinegar of Example 10 in which 3% by weight of peppermint powder was immersed.
3) 3) 레몬그라스Lemongrass 분말 powder 침지Immersion 페퍼민트 식초의 기호도 평가 Peppermint vinegar's preference rating
시험단에게 실시예 5, 14 및 16의 페퍼민트 식초를 시음하게 한 후 종합적으로 우수하다고 느낀 식초에 투표하도록 하여 하기 표 7에 나타내었다.After having the test group taste the peppermint vinegar of Examples 5, 14, and 16, they were asked to vote for the vinegar that they felt as excellent overall, and are shown in Table 7 below.
상기 표 7을 참조하면, 초산 발효 후 추가적으로 레몬그라스 분말을 침지한 실시예 14 및 16의 레몬그라스 식초가 추가적으로 레몬그라스 분말을 침지하지 않은 실시예 5의 식초보다 기호도가 높은 것으로 나타났다. 따라서, 추가적으로 레몬그라스 분말을 침지함에 따라 레몬그라스 식초의 향미가 증가하는 것을 알 수 있었다.Referring to Table 7, it was found that the lemongrass vinegar of Examples 14 and 16 additionally immersed in lemongrass powder after acetic acid fermentation was higher than the vinegar of Example 5 in which the lemongrass powder was not additionally immersed. Therefore, it was found that the flavor of lemongrass vinegar increased as the lemongrass powder was additionally immersed.
또한, 식초 총 중량 대비 3중량%의 레몬그라스 분말을 침지한 실시예 14의 식초가 5중량%의 레몬그라스 분말을 침지한 실시예 16의 식초에 비하여 기호도가 높게 나타났다. 따라서, 약 3중량%의 레몬그라스 분말을 침지할 경우 레몬그라스의 향과 맛이 과도하게 첨가되지 않아 거부감이 없는 레몬그라스 식초를 제조할 수 있음을 알 수 있었다.In addition, the vinegar of Example 14 in which 3% by weight of lemongrass powder was immersed relative to the total weight of vinegar showed higher preference than the vinegar of Example 16 in which 5% by weight of lemongrass powder was immersed. Therefore, it was found that when about 3% by weight of lemongrass powder was immersed, lemongrass vinegar without reluctance could be prepared because the aroma and taste of lemongrass was not excessively added.
Claims (9)
상기 준비된 허브 분말 30g, 고두밥 1000g, 수곡 100g, 누룩 200g, 양파즙 10g 및 정제수 4000mL를 혼합하여 25℃에서 15일간 발효하는 단계;
상기 발효를 통해 얻은 발효 주정에 상기 발효 주정 100중량부 기준 20중량부의 종초를 첨가하여 30℃에서 14일간 초산 발효하는 단계; 및
상기 초산 발효단계를 통해 얻은 초산 발효물에 페퍼민트 분말 10g, 레몬그라스 분말 10g, 로즈마리 분말 55g, 스위트베이 분말 10g 및 타임 분말 15g을 포함하는 혼합 허브 분말을 열수 추출한 혼합 허브 농축액을 초산 발효물 100 중량부를 기준으로 4중량부 첨가하는 단계를 포함하며,
상기 종초는 상기 발효 주정 중 일부에 초산균 RIC-V를 접종하여 발효시킨 것인, 허브식초 제조 방법.Preparing an herbal powder of at least one of peppermint and lemongrass;
Mixing the prepared herbal powder 30g, godubap 1000g, grain 100g, yeast 200g, onion juice 10g, and purified water 4000mL, fermenting at 25°C for 15 days;
Adding 20 parts by weight of seed seeds based on 100 parts by weight of the fermented alcohol to the fermented alcohol obtained through fermentation and fermenting with acetic acid for 14 days at 30°C; And
A mixed herb concentrate obtained by hot water extraction of mixed herb powder including 10 g of peppermint powder, 10 g of lemongrass powder, 55 g of rosemary powder, 10 g of sweet bay powder and 15 g of thyme powder to the acetic acid fermented product obtained through the acetic acid fermentation step was added to 100 weight of the acetic acid fermented product. Including the step of adding 4 parts by weight based on parts,
The seed is fermented by inoculating acetic acid bacterium RIC-V in some of the fermented alcoholic beverages.
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