KR102150826B1 - Breeding method of golden flounder to improve golden expression rate. - Google Patents

Abstract

The present invention provides a method for breeding a golden flatfish comprising: (A) a flatfish photographing step of photographing a sample flatfish for selecting the golden flatfish by using an image photographing device; (B) an image correcting and analyzing step of including a step of performing background correction of a sample flatfish image photographed in the (A) step on software and analyzing an RGB value and a CMYK value of a flatfish image; (C) a flatfish selecting step of selecting a ratio of a Y value of 45 or more among the CMKY value analyzed in the (B) step; and (D) a mating step of mating a male flatfish and a female flatfish selected in the (C) step. The present invention can develop the breeding golden flatfish capable of speeding up an expression time as generations pass; reducing an expression size; increasing the final expression rate; and increasing the number of individuals with high marketability.

Description

Breeding method of golden flounder to improve golden expression rate.}

The present invention relates to a method of breeding a golden flounder to increase the body color expression rate of a flounder having a golden color, comprising: capturing an image of the body surface of a sample flounder, correcting and analyzing the captured image, a constant among the analyzed values The present invention relates to a method for breeding a golden flounder in which the step of selecting a sample flounder including a value, and a golden flounder breeding method capable of increasing the expression time and frequency of expression of a golden fish color by crossing the male and female of the selected flounder.

Flounder ( Paralichthys olivaceus_ is one of the major fish species farmed in Asian countries, including Japan and China, and is a cultured fish that accounts for more than 45% of Korea's aquaculture production as of 2018. Flounder is mainly in the waters near Korea, China, and Japan. It is a saltwater fish that belongs to the flatfish family of the flounder inhabiting in the flounder. It can be referred to as flatfish (廣魚).

The body shape is flat by adapting to the environment under the sea, and the body color has a yellowish brown protective color that is difficult to distinguish from the sandy bottom. The body color is brown or dark on the side with the eyes, and white on the other side. One-sided eyes, a characteristic of flounder, do not appear during the fry period, but as they grow, the right eye gradually moves to the left. It usually lives in the sandy bottom of the water not exceeding 200m, and it feeds or lays eggs by changing places according to the season. Flounder living on the west coast of Korea spend winter by moving in groups to the south between autumn and winter, and when spring comes again, they migrate to the north for mating and spawning.

In recent years, due to environmental influences such as a decrease in consumption of halibut, high water temperature, and red tide, economic damage is continuously increasing and trend in farmed fish farms that raise halibut. The decrease in consumption, which is pointed out as the main cause of this phenomenon, can be confirmed through statistical data that the consumption of salmon instead of flounder is increasing rapidly. Therefore, in order to pioneer a new market for flounder, the development of new high-value-added varieties targeting Japan and China is being actively carried out.

In particular, the golden flounder is a new breed that has a yellowish golden color and is rarely found in nature with a probability of one in millions of fish, and is a high value-added variety that is traded at a price that is about 3-4 times higher than that of ordinary flounder. In addition, golden halibut boasts an excellent texture with a tacky meat quality compared to ordinary halibut, and the golden color of halibut is preferred as a good food ingredient to look at.

A number of genetic and environmental factors act as the cause of the golden body color, but the main cause is the accumulation of beta-carotene, the main natural pigment in yellow, in the body. Beta-carotene is a natural yellow pigment present in various plants, fruits, and microalgae. The present inventors are progressing technology development for mass production by selecting 12 mutant golden flounders formed with melanin pigment abnormalities, crossing them, and breeding 2,000 second-generation flatfish.

As for the conventional olive-colored mass halibut breeding method and its breeding technology, as disclosed in Korean Patent Registration No. 10-1135443, a breeding method capable of increasing the expression rate of golden halibut is not known. The same technology development is necessary.

As a method to increase the expression rate of the trait of a specific fish color, as disclosed in Korean Patent No. 10-1726812, there is a method of breeding a parent fish having a desired trait and continuing to breed its offspring. There is a problem that various factors such as heritability and environmental factors are distributed as a factor that expresses the fish body color, and the expression method is also different, so there is a problem that there is a problem that a breeding method that can increase the expression rate is needed.

In Korean Patent Registration No. 10-1135443, a group of mothers with genetic diversity is formed, from which the mothers are selected by evaluation of genetic ability and breeding of flounder through artificial insemination using artificial breeding guidelines based on genetic relationships. How to do it is disclosed. In Korean Patent Registration No. 10-1540696, halibut produced by the halibut breeding method resistant to viruses is applied to 8 microsatellite markers consisting of L3, L4, L5, L6, L8, L9, L10 and L11. The size of the alleles of the 8 loci analyzed using specific primers is 217-249 bp for L3, 288-326 bp for L4, 87-101 bp for L5, 114-140 bp for L6, 234-294 bp for L8, and 234-294 bp for L9. A method of breeding virus-resistant flounder that is resistant to viral hemorrhagic septicemia virus (VHSV) with base pairs of 80-101bp, L10 152-188bp and L11 206-254bp is disclosed. Are doing. In Korean Patent No. 10-0724805, an F1 group having 100% albino trait is selected through crossing between wild-caught albino loach parent fish, and an F2 group in which an albino trait is expressed 100% through crossing between the produced F1 groups. Disclosed is a method for mass production of albino loach, which can be selected and constructed in a mass production line with a fixed albino expression rate of 100% in all generations from wild broodstock to F1 and F2. Korean Patent No. 10-1726812 relates to a newly born hybrid by crossing a flounder and a robber leg, and a method of producing such a hybrid, and a female and a starry flounder (Platichthys) stellatus) Disclosed is a hybrid production method of halibut and strength legs, including the steps of extracting eggs and semen from males, obtaining fertilized eggs by performing artificial insemination between them, hatching and growing fertilized eggs.

The present invention is a method for breeding golden flounder in which the step of photographing an image of the body surface of a sample halibut, by crossing the male and female selected halibut by correcting and analyzing the photographed image, to increase the time and frequency of expression of the golden fish body color Want to provide.

In another embodiment of the present invention, a method for breeding golden halibut includes a crossing step (A) of crossing the selected sample halibut GF0 generation female and male golden halibut using the gold halibut breeding method to produce GF1 generation halibut progeny; A crossing step (B) of crossing the GF1 generation halibut progeny of step (A) by the golden halibut breeding method to produce GF2 generation halibut grandchildren; The GF2 generation of step (B) may be bred again in the same manner as the golden halibut breeding method to produce GF3 generation of halibut grandchildren. The criterion for selecting the sample halibut in each of the mating steps (A, B, C) is a halibut photographing step of photographing the body surface of the sample halibut using an image photographing device (A); An image correction and analysis step (b) of correcting the sample flounder image photographed in step (a) on software to analyze the color value of the fish body image as an RGB value and a CMYK value; Flounder screening step (c) of selecting a CMKY value greater than or equal to a certain ratio of the Y value among the CMKY values analyzed in step (b); It may be composed of a crossing step (D) of producing halibut by crossing the halibut female and male selected in the (c) step.

In addition, the GF0 generation may be selected for parental fish of 2 years or longer, the GF1 generation may be selected for 16 months or longer, GF2 generation may be selected for 11 months or longer, and GF3 generation may be 9 months or longer.

A method for breeding golden halibut according to an embodiment of the present invention includes a halibut photographing step of photographing a body surface of a sample halibut with an image photographing device to select the golden halibut; Image correction and color analysis step (b) including the step of correcting the image of the sample flounder photographed in step (a) on software to analyze the color value of the fish body image as an RGB value and a CMYK value; Halibut selection step (c) of selecting sample halibut having a Y value of 45% or more among the color CMKY values analyzed in step (b); It may be composed of a crossing step (D) of crossing the flounder cancer and the number selected in step (c).

The golden flounder bred by the method of the present invention increases with successive generations, the faster the time to develop the golden body color, the smaller the body size at the time of the golden color expression, and the final expression rate increases, as well as the number of individuals with high marketability.

1 shows a schematic diagram of a method for breeding golden flounder of the present invention.
2 shows a photograph of a flounder photographing step according to an embodiment of the present invention.
3 shows an RGB value and a CMYK value analysis step according to an embodiment of the present invention.
4 shows a golden flounder and a general flounder (NF) divided by the naked eye from the generation of the parent fish (GF0) to be bred according to Experimental Example 1 of the present invention.
5 shows the color concentration and overall color composition ratio of golden flounder and adult flounder according to Experimental Example 1.
FIG. 6 shows golden halibut (GF1) and general halibut, which is 500g of fish weight of 5 months old according to Experimental Example 2 of the present invention.
7 shows the result of analyzing the color density and overall color composition ratio of the first generation of golden halibut and general halibut fry according to Experimental Example 2 of the present invention.
8 shows the body color ratio between 4 types of golden flounder body color according to Experimental Example 3 of the present invention.
9 shows an example of individuals for each golden flounder group.
10 shows a table of changes in C, M, Y, and K ratios for each golden flounder group according to Experimental Example 4.
11 shows a graph of changes in C, M, Y, and K ratios for each golden flounder group according to Experimental Example 4.
12 shows a graph of the expression rate for each generation of golden flounder.
13 is a graph showing changes in product groups (A, B Type) and non-product groups (C, D Type) by generation of golden flounder according to Experimental Example 6.

The term referred to in the present invention, halibut ( Paralichthys olivaceus) is a saltwater fish belonging to the flatfish family, which mainly lives in nearby waters of Korea, China, and Japan, and may refer to flounder. The term referred to in the present invention, golden flounder may include those that stand out from among the flounder and thus represent gold and yellow.

1 shows a schematic diagram of a method for breeding golden flounder of the present invention. The golden halibut breeding method of the present invention includes a halibut photographing step (a) of photographing a sample halibut using an imaging device to select the golden halibut; An image correction and analysis step (b) comprising the step of performing background correction on the sample halibut image photographed in step (a) and analyzing the RGB and CMYK values of the fish image; Flounder screening step (c) of selecting those having a Y value of 45% or more among the CMKY values analyzed in step (b); It may consist of a crossing step (D) of crossing the female and male flounder selected in step (c). In this way, breeding is carried out by repeating steps a) through d) for each generation.

(A) Step of shooting flounder

2 shows a photograph of a flounder photographing step according to an embodiment of the present invention. The halibut photographing step (A) of the present invention is a step of photographing the surface of the fish body of the halibut using an image photographing apparatus for the sample halibut for selecting the golden halibut.

In the step of photographing halibut of the present invention, the camera is fixed using a commercial copy stand, and the halibut is placed on a white background, and then the body color of the halibut is photographed under the same conditions while irradiating white light with an intensity of 340 lux using an incandescent lamp. Analyzed. Shooting conditions were maintained at 300 ~ 400 lux using an incandescent lamp and LX1330B illuminometer, and ISO 400 with a D7100 camera (Nikon corporation, Tokyo, Japan) equipped with an 18-55 mm, F 3.5 lens (Nikon corporation, Tokyo, Japan). , Aperture F 5.6, exposure time 1/30 sec. In addition, in order to prevent errors due to unknown elements in image capturing, the standard color bar was included in image capturing for each individual and verified whether the same conditions were maintained.

(B) Image correction and analysis step

Typically, a photographed image causes color distortion due to various factors, so color correction is required accordingly. Accordingly, the image correction and analysis step of the present invention may include correcting a background using the sample flounder image captured in step (a) as software, and analyzing the RGB value and CMYK value of the fish image.

In addition, image correction and analysis according to an embodiment of the present invention may be performed through software, and the software may include a commonly known Photoshop Photoshop (Adobe Systems Incorporated, San Jose, CA).

The background correction step may include making the sample image background the same and making it transparent. The step of making the sample image background identical according to the present invention prevents the occurrence of fine differences in body color according to the shooting time, ambient light amount, and body size in accordance with the image of one or more sample images captured in step (a). This is the step of making the background the same. The step of making the image background the same according to an embodiment of the present invention may include calling an image on a Photoshop program and making the background the same based on a standard color bar.

The step of making the sample image transparent of the present invention may include making the remaining portions of the sample image transparent except for the font. In the step of making the sample image transparent according to the embodiment of the present invention, the error in the analysis of the entire body color value of the body can be reduced by duplicating the image in the Photoshop program and making the rest of the body except the body transparent using a magic tool in the copy image layer. have.

The color sensed by each image element of a typical image sensor may be determined by a combination ratio of RGB below, including the three primary colors of light: R (Red), G (Green), and B (Blue). The RGB value analysis step of the present invention is a step of analyzing the entire RGB value of the body image that has undergone the correction step using the software.

In the RGB value analysis step according to an embodiment of the present invention, only the body image is selected using a magic tool in the Photoshop program, and the R (Red) value, G (Green) value, and B (Blue) value are examined in the color in the bar graph in the navigator. It may include the step of.

In general, the process of setting the output characteristic of a display device to a reference color or another device is called color calibration, and is widely used to accurately express the color to be printed. Since the monitor is an RGB (red, green, blue) expression method, color correction is performed to print with a printer using CMYK (cyan, magenta, yellow, black) ink. Color correction works by using color lookup table values.

In general, color input/output devices that reproduce colors such as monitors, scanners, cameras, printers, etc. use different color spaces or color models according to each field of use. For example, in the case of color images In the printing apparatus, CMY or CMYK color space is used, and CMKY of the present invention may refer to a chart in which chroma, brightness, and hue are varied in the conventional color space.

The CMYK analysis step of the present invention is a step of analyzing a plurality of CMYK values corresponding to the analyzed RGB color values. 3 shows steps of analyzing RGB values and analyzing CMYK values according to an embodiment of the present invention. In the CMYK value analysis step according to an embodiment of the present invention, C (cyan), M (magenta), Y (yellow), and K (black) values can be analyzed by inputting the obtained RGB value from a color picker in a Photoshop program. have. Thereafter, to preserve and verify color values, a square tool can be created outside the image and filled with the same color as the RGB and CMYK values using a paint tool.

(C) Flounder selection step

The flounder selection step of the present invention is an individual with 45% or more based on the Y value among the CMYK values obtained through the step (b), 43% ≤ Y <45%, 41% ≤ Y <43%, and Y <41%. This is the step of sorting and selecting. In general, the CMKY value is expressed as% in image software, but in the present invention, the total amount value obtained by summing each CMKY value expressed as% in the image measurement of the selected halibut is the denominator and the ratio is calculated using the Y value as the numerator. Was used as the selection criteria range.

(D) mating stage

The mating step of the present invention includes the step of breeding by crossing the female or male halibut having a predetermined value or more selected through the step (c). Subsequently, the produced individual is grown and re-selected breeding is repeated through crossing between the same generation through classification of body color values. Hereinafter, a specific experimental example will be described.

< Experimental example 1> Golden flounder With the parent fish generation Common flounder Body color comparison

After performing the steps (a) to (b) of the present invention, a body color comparison between golden flounder (GF-0) and general flounder (NF) adult fish was performed. 4 shows a golden flounder and a general flounder (NF) divided by the naked eye from the generation of the parent fish (GF0) to be bred according to Experimental Example 1 of the present invention.

Table 1 below shows RGB and CMYK values, and FIG. 5 shows the color density and overall color composition ratio of the golden flounder and general flounder adult fish according to Experimental Example 1. As a result of the comparison, it was judged that the criterion for distinguishing between golden halibut and general halibut could be determined. As an example, it can be defined as an individual having a density of Y of 85% or more and a density of K of 50% or less among the color elements, but it is considered that the number of target golden flounders is still small, so it will change according to the change of statistics.

Color components of golden flounder and common flounder Color element Golden flounder
GF 0 Common flounder
NF Color element Golden flounder
GF 0 Common flounder
NF R 116±23.1 60±9.8 C 40±6.0 53±5.2 G 75±14.1 38±5.4 M 64±3.3 69±3.9 B 31± 7.3 24±2.3 Y 96±5.9 78±2.5 K 36±12.1 67±4.4

< Experimental example 2> Golden flounder 1st generation (GF1) and general flounder ( NF ) Of cheerleader Body color comparison

The first generation of golden halibut (hereinafter, GF1) according to the present invention selects the gold halibut pro-fish generation (GF0) selected according to Experimental Example 1 according to steps (a) to (c) of the present invention, and the selected golden halibut Refers to the offspring of a male (♂) and a female golden flounder (♀).

According to Experimental Example 2 of the present invention, in the case of the GF1 generation, genetically, it is expected that golden expression will occur with growth, and the present invention is each 10 rice compared to a general flounder of similar size of the same age (5 months of age, 500 g of fish weight). Photographed and analyzed by the method according to steps (A) to (B) of.

Figure 6 shows the weight of the fish weight 500g gold halibut (GF1) and general halibut according to Experimental Example 2 of the present invention Table 2 and Figure 7 is the first generation of gold halibut and general halibut according to Experimental Example 2 of the present invention The analysis results of the density and overall color composition ratio of each color are shown.

GF1 was visually judged to be yellow compared to general flounder, but when analyzed by color factor, it was confirmed that the rest of the elements except C were somewhat higher. Among them, the Y element showed the largest difference in width and the overall composition ratio was also the largest.

Same color component of the age-old flatfish photo as GF1 Color element Golden flounder
GF1 Common flounder
NF Color element Golden Flounder GF1 Common flounder
NF R 51±1 69±5 C 43±1 49±2 G 84±2 89±6 M 72±1 65±1 B 46±1 63±5 Y 87±1 69±2 K 57±1 46±4

< Experimental example 3> Golden flounder Grouping

The CMYK values analyzed by photographing the golden flounder according to Experimental Examples 1 and 2 were converted into ratios and compared. As a result of analyzing the color components of the photograph of the golden flounder, the concentrations of cyan (C) and magenta (M) were not significantly different, ranging from 50 to 52%, whereas the density of yellow (Y) and black (K) differed from each other. It was confirmed that the difference remained outside the range.

Accordingly, grouping was performed into A, B, C, and D (4 Type) according to the yellow (Y) ratio, and Table 3 below shows the grouping criteria, and FIG. 8 shows the body color of the golden flounder according to Experimental Example 3 of the present invention. The body color ratio between the 4 types is shown, and FIG. 9 shows an example of an individual for each golden flounder group.

Criteria for grouping A, B, C, D according to the yellow (Y) ratio A Type

Y45.00

Y B Type 43.00

Y <45.00 C Type 41.00

Y <43.00 D Type Y <41.00

< Experimental example 4> Golden flounder By group Body color change

Changes in body color for each golden flounder group classified according to Experimental Example 3 were confirmed. Normally, the golden flounder began to change from normal body color to golden body color, and took about 10 to 12 weeks, and there was little change thereafter.

Experimental Example 4 of the present invention confirmed the change in body color for each golden flounder group at the time point at which the body color changes. 10 shows a table of changes in C, M, Y, and K ratios for each golden halibut group according to Experimental Example 4, and FIG. 11 shows a graph of C, M, Y, and K ratio changes for each golden halibut group according to Experimental Example 4. The C ratio was 28~30%, the M ratio was 29~30%, the Y ratio was 30~32%, and the K ratio was 9~11%.There was no difference between groups, but the Y ratio increased by group over time. It was confirmed that there was a difference in the amount of reduction between the and K ratio.

< Experimental example 5> Golden flounder Expression rate by generation

The first generation of golden halibut (hereinafter, GF1) according to Experimental Example 5 of the present invention includes the first generation of golden halibut (GF0) selected according to Experimental Example 1 of Experimental Example 1 in steps (a) to (c) of the present invention. It refers to the offspring of crossing the selected male golden halibut (♂) and female golden halibut (♀), and the second generation of golden halibut (hereinafter, GF2) refers to the male golden halibut selected by the same process as the GF1 generation ( ♂) and female golden flatfish (♀), and the third generation of golden flatfish (hereinafter, GF3) refers to male golden flatfish (♂) and female golden flatfish (♀) selected by the same process as the GF2 generation. It refers to the generation of descendants that have crossed

Table 5 below shows the results of the expression rate survey for each generation, and FIG. 12 shows a graph of the expression rate for each generation of golden flounder. GF1 generation was produced in April 2015, and the first expression was found 15 months later, and the expression rate was 20.5% until 20 months. GF2 generation was produced in August 2016 and first appeared at 9 months, showing 42.50% by 20 months. GF3 generation was produced in June 2018 and began to appear after 8 months, and until 14 months (August 19), it showed a higher expression rate than the previous generation at 45.93%.

Results of the expression rate survey by generations Age GF-1 generation GF-2 generation GF-3 generation Expression rate (%) Weight (kg) Expression rate (%) Weight (kg) Expression rate (%) Weight (kg) 8 months - - - - 0.11 0.23 9 months - - 0.00 0.27 0.36 0.28 10 months - - 0.15 0.28 2.95 0.36 11 months - - 0.70 0.33 17.46 0.40 12 months - - 2.55 0.46 33.83 0.47 13 months - - 6.05 0.53 41.59 0.57 14 months - - 12.50 0.62 44.23 0.60 15 months 0.03 0.92 16.90 0.73 47.03 0.68 16 months 0.70 1.05 22.35 0.87 50.13 0.79 17 months 7.00 1.52 27.15 0.94 54.06 0.89 18 months 10.40 1.79 32.88 1.10 57.03 0.98 19 months 15.20 1.95 36.98 1.10 59.98 1.10 20 months 20.50 2.10 42.50 1.15 62.39 1.22

Table 6 below shows the comparison results of expression patterns by generation of golden flounder. The time of expression is the time of expression when the expression rate of each generation is 0.7%, and the GF0 generation is based on the time point of discovery and recruitment in nature or in other farms. In addition, as for the final expression rate, the expression rate up to 20 months after production by generation was considered as the final expression rate.

Comparison of expression patterns by generation of golden flounder Time of manifestation Expression weight (kg) Final expression rate (%) Remark GF- 0 generation 2 years or more Application time GF- 1st generation 16 months 1.05 20.5 GF- 2nd generation 11 months 0.33 42.5 GF- 3rd generation 9 months 0.28 62.4

As the golden flounder goes through generations, the onset of manifestation is getting faster from 15 months to 9 months to 8 months, and the time of manifestation is also getting faster. It can be seen that as the time of expression increases, the expressed weight is also lowering to 1.05kg of GF1 generation, 330g of GF2 generation, and 280g of GF3 generation. In addition, it was confirmed that the generational expression rate increased to 20.5% of the GF-1 generation and 42.5% of the GF-2 generation.

< Experimental example 6> Golden flounder By generation Body color Group change

In Experimental Example 6 of the present invention, the body colors for each generation of golden flounder according to Experimental Example 5 were grouped in the same manner as in Experimental Example 3. The GF0 generation was investigated on the development of 12 parent fish, and 135, 250, and 400 animals were sampled and investigated in GF1 to 3 generations.

Table 7 below shows the body color ratio between 4 types of golden flounder body color according to Experimental Example 6, and FIG. 13 is a product group (A, B Type) and non-product group (C, D Type) by generation of gold flounder according to Experimental Example 6. Show change graph.

Body color ratio between 4 types of golden flounder body color GF-1 generation GF-2 generation GF-3 generation Mari number % Mari number % Mari number % Prize

Width A Type 88 65.2 184 73.6 305 76.3 B Type 20 14.8 36 14.4 52 13.0 sub Total 108 80.0 220 88.0 357 89.3 ratio
Prize
Width C type 19 14.1 17 6.8 26 6.5 D type 8 5.9 13 5.2 17 4.3 sub Total 27 20.0 30 12.0 43 10.8 Sum 135 100.0 250 100.0 400 100.0

As an A-Type product, the ratio of the best products is increasing from 65.2% in the GF1 generation to 76.3% in the GF-3 generation. And B-Type, which can be sold as a product, showed little change in ratio until GF-1 to 3rd generation.

However, the proportion of C and D type that cannot be sold as a product is decreasing. As the generations of the golden flounder increase, not only the ratio of the golden body color is increasing, but the number of individuals that can be sold as a product is increasing as the golden body color becomes clear.

According to Experimental Example 6 of the present invention, it was confirmed that the golden flounder bred according to the method of the present invention increases with generations, the faster the expression time, the smaller the expression size, the higher the final expression rate, and the higher the number of individuals with high marketability. .

The present invention has industrial applicability because it is possible to breed golden halibut, which is judged to be of high added value in the market, to reduce the production cost of halibut and to improve the marketability of halibut.

Claims (2)

GF1 generation halibut progeny crossing step (A) generated by crossing female and male GF0 generation halibut selected by collecting golden halibut (A);
GF2 generation halibut progeny crossing step (B) of reselecting and crossing the GF1 generation halibut progeny of step (A);
Including a crossing step (C) of GF3 generation halibut progeny to reselect and cross GF2 generation halibut progeny of step (B),
In the selection of the flounder in any one of the mating steps, the body surface of the flounder is photographed using an image photographing device, and the captured flounder image is corrected on software to analyze the color value of the flounder image as an RGB value and a CMYK value, Golden halibut breeding method, characterized in that among the analyzed CMKY values, halibut having a Y value of 43% or more is selected
The body surface of the flounder was photographed with an image capturing device, and the captured flounder image was corrected in software to analyze the color value of the flounder image as an RGB value and a CMYK value. Among the analyzed CMKY values, the ratio of the Y value was 43 Gold flounder breeding method, characterized in that the selected flounder is crossed with the selected flounder by selecting% or more.

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