KR102136422B1 - Antimicrobial composition for poultry comprising green tea saponin - Google Patents

Abstract

The present invention provides a poultry antibacterial composition containing green tea saponin E1, C1 or E3 as an active ingredient, which effectively suppresses strains of Salmonella that cause diseases such as diarrhea of poultry with excellent antibacterial activity.

Description

An antimicrobial composition for poultry comprising green tea saponin

The present invention relates to a poultry antimicrobial composition, and more particularly, to a poultry antimicrobial composition containing green tea saponin as an active ingredient.

Green tea is harvested from the Camellia sinensis and contains various pharmacologically active compounds such as catechin, caffeine, saponin, kaempferol, naringenin, theanine, vitamins, oil minerals and trace elements (Khan). , A. et al., Asian Journal of Experimental Biological Science , 3:459-467, 2012) In particular, saponin glycosides contained in green tea seeds are sterol or triterpenoid. And a soluble aglycone composed of a chain of water-soluble glycones (Rao, AV and Gurfinkel, DM, The Journal of Nutrition, 125: 211-236). The saponin has been known to have many physiological activities such as hemolytic action, antibacterial action, antifungal action, anticancer action, adjuvant action, gastric protection action (Woldemichael, GM and Wink, M., Journal of Agriculture Food Chemistry , 49:2327) -2332, 2001; Francis, G. et al., British Journal of Nutrition , 88:587-605; Guclu-Ustundag, O. and Mazza, G., Critical Reviews in Food Science and Nutrition , 47:231-258, 2007). The physiological activity of the saponin is influenced by factors such as the morphology of the saponin nucleus, the number of sugar chains, and the type of functional group (Osbourn, AE, et al., Phytochemistry , 62:1-4, 2003). Republic of Korea Patent Publication No. 2013-0108853 discloses a composition containing a triterpenoid saponin extracted from the root of the tea tree, Republic of Korea Patent No. 0771399 discloses an increase in oxidative stress and cholesterol containing green tea seed extract as an active ingredient It discloses a pharmaceutical composition for the prevention and treatment of diseases caused by. In addition, US Patent Publication No. US2013-0259952 discloses TIE2 activation, vascular endothelial growth factor (VEGF) inhibitors, angiogenesis inhibitors, vascular maturation inhibitors, vascular normalizing agents and stabilizers and pharmaceutical compositions. Meanwhile, Boran et al. (Turk. J. Fish. Aqua. Sci., 15: 49-57, 2015) reported that green tea seed hydrothermal extract or saponin has antibacterial activity against fish's pathogenic microorganism Listonella anguillarum . Bar, Kuo et al . ( J. Agric. Food Chem ., 58(15): 8618-8622, 2010) reported that saponins derived from green tea seeds have antibacterial activity against the phytopathogenic fungus Rhizoctonia solani . have. In this regard, Republic of Korea Patent No. 1872760 discloses an antibacterial composition and its use as a green tea seed saponin as an active ingredient.

However, in the case of the prior art, green tea seeds extracted from defatted green tea seeds provide an antimicrobial composition effective for preventing and treating tooth decay and preventing and treating diarrhea diseases of livestock and poultry as an active ingredient of total saponin, and contain a specific saponin component as an active ingredient Studies on poultry antimicrobial compositions with superior effects are still insignificant.

The present invention is to solve a number of problems, including the above problems, to provide a poultry antibacterial composition containing green tea saponin E1, C1 or E3 as an active ingredient showing excellent antibacterial activity against various Salmonella genus strains Is done. However, these problems are exemplary, and the scope of the present invention is not limited thereby.

According to one aspect of the present invention, there is provided an antimicrobial composition for controlling Salmonella bacteria containing chasaponin E1, C1 or E3 as an active ingredient.

According to another aspect of the present invention, removing the outer skin of the dry green tea seeds and grinding to powder; After adding an organic solvent to the green tea seed powder, applying ultrasonic waves to remove the oil and dry the components; Adding C1 to C3 lower alcohol or an aqueous solution thereof to the defatted green tea seed powder to obtain irradiation saponin; And the irradiation solution of initial (0 min) methanol (A) and water (B) as the mobile phase (A 74: B 26), after 30 minutes A 75: B 25, after 45 minutes A 100: B 0 A method for separating and purifying chasaponin E1 from green tea seeds comprising evaporating the solvent after recovering the third fraction (26-27 minutes) of the fractions separated by HPLC using a method is provided.

According to another aspect of the present invention, there is provided a composition for adding feed for preventing poultry diarrhea, which contains tea saponin E1, C1 or E3 as an active ingredient.

According to an embodiment of the present invention made as described above, the poultry antimicrobial composition containing green tea saponin E1, C1 or E3 as an active ingredient exhibits excellent antimicrobial activity to control a strain in the genus Salmonella that causes poultry diarrhea. It is very effective. Of course, the scope of the present invention is not limited by these effects.

1 is a photograph showing the saponin TLC form of green tea seeds and leaves of the present invention.
2 is an HPLC chromatogram graph of the green tea saponin fraction of the present invention.
3 is a graph analyzing the pattern of green tea saponins of the present invention by LC-MS.
Figure 4 is a diagram showing the structure of the green tea saponin fraction of the present invention.
5 is a graph analyzing the cytotoxicity of green tea saponin E1 of the present invention.
6 is a graph analyzing the cell viability of green tea saponin E1 of the present invention.
7 is a photograph of the antibacterial effect of green tea saponins E1, E3 and C against various indicator strains.
8 is a graph analyzing the antibacterial effect of green tea saponin E1.
9 is a graph analyzing the antibacterial effect of green tea saponin C.
10 is a graph analyzing the antibacterial effect of green tea saponin E3.

Definition of Terms:

The term "saponins" used in this document is a glycoside of steroid, steroid alkaloid, or triterpene, which is a generic term for a substance that dissolves in water and exhibits a soapy foaming action. It exists in many plants and is also contained in the body of some echinoderm (starfish, sea cucumber). Saponins are known to increase the body's immunity. In particular, it is known that ginsenosides and eleutherosides, which are the types of saponins contained in ginseng, are known, and green tea also has various types of saponins.

Detailed description of the invention:

According to one aspect of the present invention, there is provided an antimicrobial composition for controlling Salmonella bacteria containing chasaponin E1, C1 or E3 as an active ingredient.

In the antimicrobial composition, the saponin E1 is {(2S,3S,4S,5R,6R)-6-[[(3S,4S,4aR,6aR,6bS,8R,8aR,9R,10R,12aS,14aR, 14bR)-9-acetyloxy-4-formyl-8-hydroxy-8a-(hydroxymethyl)-4,6a,6b,11,11,14b-hexamethyl-10-[(Z)-2-methylbut-2-enoyl] oxy-1,2,3,4a,5,6,7,8,9,10,12,12a,14,14a-tetra-decahydropicen-3-yl]oxy]-4-[(2S,3R,4S ,5S)-4,5-dihydroxy-3-[(2S,3R,4S,5R)-3,4,5-trihydroxyoxan-2-yl]oxyoxan-2-yl]oxy-3-hydroxy-5-[ (2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxane-2-carboxylic acid}.

In the antibacterial composition, the saponin E3 is (3β,16α,21β,22α)-16,22,28-Trihydroxy-21-{[(2Z)-2-methyl-2-butenoyl]oxy}-23-oxoolean -12-en-3-yl β-D-galactopyranosyl-(1->2)-[β-D-xylopyranosyl-(1->2)-α-L-arabinopyranosyl-(1->3) ]-β -D-glucopyranosiduronic acid.

In the antimicrobial composition, the saponin C1 is Angeloyl, -O-[β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl-(1→3)-[β-D-galctopyranosyl-(1→ 2)]-β-D-glucopyranoside].

According to another aspect of the present invention, removing the outer skin of the dry green tea seeds and grinding to powder; After adding an organic solvent to the green tea seed powder, applying ultrasonic waves to remove the oil and dry the components; Adding C1 to C3 lower alcohol or an aqueous solution thereof to the defatted green tea seed powder to obtain irradiation saponin; And the irradiation solution of initial (0 min) methanol (A) and water (B) as the mobile phase (A 74: B 26), after 30 minutes A 75: B 25, after 45 minutes A 100: B 0 A method for separating and purifying chasaponin E1 from green tea seeds comprising evaporating the solvent after recovering the third fraction (26-27 minutes) of the fractions separated by HPLC using a method is provided.

In the separation and purification method, the organic solvent may be n-hexane, ethanol, methanol, acetone, ethyl acetate, ethylene glycol or benzene, and the lower alcohol may be methanol, ethanol, or propanol.

According to another aspect of the present invention, there is provided a composition for adding feed for preventing poultry diarrhea, which contains tea saponin E1, C1 or E3 as an active ingredient.

According to published data, some plant saponins have antioxidant properties and are emerging as new antioxidants. Saponins are one of the most diverse groups of substances in nature, and a significant number of saponins have long been used and have been well known as folk remedies. Saponin is a natural compound with a wide range of biological effects and medicinal value, which is an ideal material for use in cosmetics due to its important bioactivity, and green tea saponin has been reported with many biological effects, including antibiotics, anticancer drugs, immune supplements, and gastric protective functions. have. The biological activity of saponins depends on the chemical structure and is influenced by factors such as the molecular structure of saponins, the number of sugar chains, and the type of functional substituents.

The present inventors have developed an antimicrobial composition that uses green tea seed saponin as an effective ingredient for the prevention of tooth decay and the prevention and treatment of diarrhea in livestock and poultry (Application No. 10-2015-0104634). However, as a result of applying a single component of green tea saponin E1 to various indicator strains, despite the small amount of treatment, the antibacterial activity is superior to that of the green tea seed saponin, and in particular, it is very effective in suppressing the strain of Salmonella genus. A poultry antibacterial composition containing E1 as an active ingredient was completed.

The composition of the present invention may be administered orally or parenterally, and when administered parenterally, it is possible to administer via various routes such as intravenous injection, intranasal inhalation, intramuscular administration, intraperitoneal administration, and percutaneous absorption.

In addition, the composition of the present invention is administered in a pharmaceutically effective amount. The term "pharmaceutically effective amount" as used herein refers to an amount sufficient to treat the disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the individual type and severity, age, sex, and drug It can be determined according to the activity of the drug, the sensitivity to the drug, the time of administration, the route of administration and discharge, the duration of treatment, factors including the drugs used simultaneously, and other factors well known in the medical field. The pharmaceutical composition of the present invention may be administered in a dose of 0.1 mg/kg to 1 g/kg, more preferably 1 mg/kg to 500 mg/kg. Meanwhile, the dosage may be appropriately adjusted according to the patient's age, gender and condition.

The pharmaceutical composition according to an embodiment of the present invention further comprises an inert component, including a pharmaceutically acceptable carrier. “Pharmaceutically acceptable carrier” as used herein is a term referring to components excluding the active substance of a composition, specifically a pharmaceutical composition. Examples of pharmaceutically acceptable carriers include binders, disintegrants, diluents, fillers, lubricants, solubilizers or emulsifiers and salts. In addition, the pharmaceutical composition according to an embodiment of the present invention may be administered in a dose of 0.1 mg / kg to 1 g / kg, more preferably in a dosage of 0.1 mg / kg to 500 mg / kg . Meanwhile, the dosage may be appropriately adjusted according to the patient's age, gender and condition.

In the feed additive of the present invention, other additives may be additionally included in addition to the green tea seed saponin, and these additives include organic acids such as citric acid, humic acid, adipic acid, lactic acid, and malic acid, sodium phosphate, potassium phosphate, acid pyrophosphate, polyphosphate ( Phosphates such as polymerized phosphates) or any one of natural antioxidants such as polyphenols, catechin, alpha-tocopherol, rosemary extract, vitamin C, green tea extract, licorice extract, chitosan, tannic acid, and phytic acid It may contain additional abnormalities, and may include various amino acids, inorganic salts, vitamins, antibiotics, antibacterial substances, antioxidants, antifungal enzymes, living probiotics, etc. as nutritional supplements, and improve digestion and absorption, growth promoters, etc. It may also be included additionally. In addition, the feed additive may be administered to animals alone or in combination with other feed additives in an edible carrier.

Hereinafter, the present invention will be described in more detail through examples. However, the present invention is not limited to the embodiments disclosed below, but can be implemented in various different forms, and the following embodiments make the disclosure of the present invention complete, and the scope of the invention to those skilled in the art It is provided to inform you completely.

Example 1: Disclosure Materials

B16-F10 (mouse melanoma cells; ATCC Number: CRL-6322™) and human embryonic kidney cells (HEK-293; ATCC CRL-157), primary human foreskin dermal fibroblasts are purchased from ATCC Did.

Example 2: Green tea seed extraction

Green tea ( Camellia sinensis ) seeds were collected from the famous Shin Kwang-soo green tea garden (Suncheon, Korea) for 11-December and dried green tea seeds were removed and ground to powder. After adding 4 L of n-hexane to green tea seed powder (2 kg), ultrasonic waves were applied at 30° C. for 5 hours, and then the fat and oil components were removed and dried. The green tea seed powder thus degreased was further extracted with 70% ethanol at 60°C for 4 hours to obtain 89 g of irradiated saponin. Irradiated saponin thus obtained was filtered, concentrated in a rotary vacuum evaporator (SB-100, Eyela), lyophilized and quantified.

Example 3: Extract from green tea leaves

Green leaves ( Camellia sinensis ) The leaves harvested from Shin Kwang-soo Green Tea Garden (Suncheon, South Korea), which are famous for their leaves, are dried or crushed using 5 kg of leaves as they are. Extraction with 70% ethanol at 60° C. for 4 hours yielded 40 g and 30 g of irradiated saponin, respectively. The obtained irradiation saponin was filtered, concentrated in a rotary vacuum evaporator (SB-100, Eyela), lyophilized and quantified, and each was subjected to TLC.

As a result, the green tea seeds were found to contain a lot of saponin E1, and the leaves were extracted without drying and then the leaves extracted after drying showed a difference in the amount of green tea saponin E1 (Fig. One). Therefore, it is considered that it can be selected from green tea seeds or green tea leaves from an economical point of view.

Example 4: HPLC chromatogram

The present inventors obtained a total of six types of green tea seed saponin fractions using HPLC. Specifically, using a preparative HPLC system Phenomenex (21.2 mm x 250 mm, 5 μ) column, 7 mL of pump, Shimadzu LC-6AD was used as the mobile phase, A: methanol B: aqueous solution (74: 26) was used, and it was adjusted to methanol:water (75:25) at 30 minutes and methanol:water (100:0) at 45 minutes. Fractions were 21.38 minutes (Fr.1), 24.49 minutes (Fr.2), 26.34 minutes (Fr.3), 28.79 minutes (Fr.4), 31.52 minutes (Fr.5) and 34.22 minutes (Fr.6). Obtained (FIG. 2). The saponin content of green tea seeds of the obtained 6 fractions is summarized in Table 1 below.

Saponin content of 6 fractions Classification Saponin content Fr.1 1.4 g Fr.2 2.1 g Fr.3 4.3 g Fr.4 2.5 g Fr.5 1.2 g Fr.6 2.4 g

In addition, thin layer chromatograms were performed using different solvents to obtain RF values of the total saponin (TS) and saponin fractions. Specifically, the solvent system was mixed with n-butanol:water:acetic acid (84:14:17), sprayed and immersed using 10% H2SO4, and the reversephase solvent system was methanol:water (4: After mixing with 1), it was sprayed with 10% H ₂ SO ₄ . The RF values of the total saponin (TS) and saponin fractions are summarized in Table 2 below.

RF values of saponin (TS) and saponin fraction compound Rf value (n-butanol:water:acetic acid (84:14:17)) Rf value
(Methanol:water (4:1)) Spray Dipping 0.88 TS 0.14 0.5 0.88 Fr.1 0.14 0.45 0.86 Fr.2 0.14 0.45 0.88 Fr.3 0.17 0.50 0.88 Fr.4 0.17 0.50 Fr.5 0.20 0.50 Fr.6 0.19 0.50

Example 5: Structure determination of saponins

The inventors determined the structures of fractions 1, 2 and 3 of the six fractions obtained through Example 4. Specifically, an agilent extend-C18 (5 μm 2.1×150 mm) column was used for LC-MS (Thermo LTQ-orbitrap-XL) and acetonitrile (A: 0.1% Formic acid (FA)) and water (B) as the mobile phase. : 0.1% FA) was used and the pattern of green tea seeds saponin was analyzed by applying the gradient conditions in Table 3 below.

As a result, the morphology of LC-MS was confirmed to be a standard product, a hydrolysis type, and different patterns of green tea seed saponins (FIG. 3). In addition, based on the pattern analysis, the structures of fractions 1, 2, and 3 were determined, and fraction 1 was determined as Teasaponin C1, fraction 2 was Teasaponin E3, and fraction 3 was determined as Teasaponin E1 (FIG. 4). The gradient conditions for the saponin pattern analysis are summarized in Table 3 below, and ¹³ C NMR data of Fraction 2 is shown in Table 4, ¹³ C NMR data of Fraction 3 is shown in Table 5, and ¹³ C NMR data of Fraction 1 is shown. It is summarized in Table 6 below.

LC-MS gradient conditions time A% B% Flow (ml/min) 0.00 74.0 26.0 7.0 30.00 75.0 25.0 7.0 31.00 80.0 20.0 7.0 33.00 85.0 15.0 7.0 35.00 90.0 10.0 7.0 45.00 100.0 10.0 7.0

¹³ C NMR data of fraction 2 ¹³ C-NMR data of 544-3 in pyridine- d ₅ location Fraction 2 Standard product (tea saponin E ₃ ) location Fraction 2 Standard product (tea saponin E ₃ ) One 38.5 38.2 One' 104.5 104.2 2 25.6 25.3 2' 78.5 78.3 3 84.4 84.1 3' 84.9 84.5 4 55.5 55.2 4' 71.4 70.8 5 48.7 48.2 5' 77.5 77.3 6 20.7 20.4 6" 171.1 172.0 7 32.7 32.4 One" 103.6 103.3 8 40.6 40.3 2" 74.0 73.7 9 47.1 46.9 3" 75.7 75.4 10 36.4 36.2 4" 70.8 70.4 11 24.1 23.8 5" 76.9 76.5 12 123.4 123.1 6" 62.4 62.1 13 143.1 143.6 One"' 101.9 101.7 14 42.1 41.9 2"' 82.3 82.4 15 34.9 34.4 3"' 73.7 73.4 16 68.2 67.8 4"' 68.6 68.3 17 47.6 47.8 5"' 66.7 66.6 18 40.8 40.5 One"" 107.4 107.1 19 47.4 47.0 2"" 75.7 75.9 20 36.3 36.1 3"" 78.5 78.3 21 81.5 81.7 4"" 71.1 70.8 22 73.6 73.1 5" 67.8 67.5 23 210.4 209.9 One""' 168.9 168.7 24 11.4 11.1 2""' 129.8 129.6 25 16.1 15.8 3""' 136.3 136.0 26 17.3 16.9 4""' 16.2 15.9 27 27.7 27.4 5""' 21.4 21.1 28 66.3 66.0 29 30.1 29.9 30 20.6 20.4

¹³ C NMR data of fraction 3 ¹³ C-NMR data of 544-4 in pyridine- d ₅ location Fraction 3 Standard product (tea saponin E ₁ ) location Fraction 3 Standard product (tea saponin E ₁ ) One 38.5 38.3 One' 104.4 104.1 2 25.5 25.2 2' 78.6 78.4 3 84.8 84.5 3' 84.1 84.2 4 55.4 55.2 4' 71.2 70.8 5 48.7 48.4 5' 76.9 77.3 6 20.7 20.4 6" 171.3 171.8 7 32.7 32.5 One" 103.6 103.2 8 40.6 40.4 2" 74.0 73.7 9 47.1 46.8 3" 75.7 75.3 10 36.4 36.1 4" 70.8 70.5 11 24.1 23.8 5" 76.8 76.5 12 123.4 123.1 6" 62.4 62.1 13 143.3 142.9 One"' 102.0 101.7 14 42.0 41.8 2"' 82.3 82.3 15 34.9 34.6 3"' 73.8 73.4 16 68.2 68.1 4"' 68.7 68.4 17 48.3 48.0 5"' 66.5 66.1 18 40.4 40.2 One"" 107.4 107.0 19 47.5 47.2 2"" 76.3 75.9 20 36.6 36.3 3"" 78.5 78.2 21 79.2 78.9 4"" 70.8 70.8 22 74.6 74.5 5" 68.0 67.5 23 210.3 209.8 One""' 168.2 167.9 24 11.4 11.0 2""' 129.3 129.0 25 16.1 15.8 3""' 137.5 137.0 26 17.1 16.9 4""' 16.3 15.9 27 27.7 27.4 5""' 21.4 21.0 28 64.1 64.0 One""" 170.4 171.1 29 29.8 29.5 2""" 21.2 20.9 30 20.6 20.3

¹³ C NMR data of fraction 1 ¹³ C-NMR data of 551G3-1 in pyridine- d ₅ location Fraction 1 Standard product (charsaponin C ₁₎ location Fraction 1 Standard product (charsaponin C ₁₎ One 39.0 38.7 One' 104.4 104.1 2 25.8 25.5 2' 78.8 78.5 3 83.3 83.1 3' 84.6 84.6 4 43.8 43.5 4' 72.0 71.0 5 48.4 48.2 5' 76.4 77.4 6 18.4 18.2 6" 172.0 172.0 7 33.1 32.8 One" 103.4 103.2 8 40.4 40.1 2" 74.0 73.8 9 47.3 47.0 3" 75.5 75.3 10 37.0 36.8 4" 70.4 70.1 11 24.1 23.9 5" 76.8 76.5 12 123.4 123.1 6" 62.2 61.9 13 144.0 143.7 One"' 101.9 101.7 14 41.9 41.6 2"' 82.6 82.3 15 35.4 35.2 3"' 73.7 73.4 16 70.4 70.1 4"' 68.6 68.3 17 45.1 44.8 5"' 66.9 66.6 18 41.2 40.9 One"" 107.3 107.1 19 47.7 47.4 2"" 76.2 75.9 20 32.3 32.1 3"" 78.5 78.3 21 42.0 41.7 4"" 71.1 70.8 22 73.3 73.0 5" 67.8 67.5 23 65.1 64.8 One""' 168.3 168.0 24 13.9 13.6 2""' 129.8 129.5 25 16.5 16.2 3""' 136.9 136.6 26 17.2 16.9 4""' 16.2 15.9 27 27.9 27.6 5""' 21.3 21.0 28 63.9 63.6 29 33.7 33.5 30 25.5 25.2

Example 6: Cell viability analysis

The present inventors analyzed the functionality of green tea saponin E1 (GTS E1) with the most effective amount among the fractions obtained through the above examples. Specifically, for cell culture, fetal bovine serum (FBS; Gibco, NY, USA), penicillin in which B16-F10 (mouse melanoma) cells and HEK-293 cells were inactivated with 10% heat in Dulbecco's Modified Eagle Medium (DMEM) medium (100 unit/ml) and streptomycin (100 mg/ml) were added and cultured at 5% CO ₂ and 37° C. conditions. Thereafter, the cultured cells were seeded in a 96-well plate at 7.5ㅧ10 ³ cells/well, and green tea saponin E1 at various concentrations (5 to 100 µg/mL) was added to incubate for 48 hours and treated with 0.1% DMSO. The experimental group was used as a negative control. Then, after treating the MTT (3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide) solution, the mixture was incubated at 37°C for 3 hours, and the formed purple formazan was isopropanol. Melt with and measure the absorbance at 590 nm.

As a result, it was confirmed that the cells of the experimental group in which the concentration of green tea saponin E1 was treated to 100 μg/mL survived at least 90%, confirming that the green tea saponin E1 of the present invention is a safe natural product (FIG. 5 ). In addition, as a result of comparing the green tea saponin E1 cytotoxicity to B16-F10 cells and HEK-293 cells with the control group, the survival rate of 90% or more was considered to be almost non-toxic (FIG. 6).

Example 7: Analysis of antibacterial activity

According to an embodiment of the present invention, the antibacterial activity of green tea saponins E1, E3 and C1 obtained above was measured. Specifically, S. aureus, S. dublin, S. cholera, S. typhimurium, S. gallinarum, S. enteritidis, S. Pullorum , and E. coli were used to measure antimicrobial activity. By measuring the clear zone that inhibits the growth of this strain, the degree of inhibition of the strain was expressed as the diameter of the clear zone.

As a result, it was confirmed that the green tea saponin E1 of the present invention shows the highest antibacterial activity against various Salmonella genus strains compared to green tea saponins E3 and C1 (FIGS. 7 to 10 ).

The present invention has been described with reference to the above-described embodiments, but these are merely exemplary, and those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. Therefore, the true technical protection scope of the present invention should be determined by the technical spirit of the appended claims.

Claims (5)

An antibacterial composition for controlling Salmonella, which contains chasaponin E1, C1 or E3 as an active ingredient. delete delete delete Chasaponin E1, C1 or E3 containing as an active ingredient poultry diarrhea disease prevention feed additive composition.

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