KR102130245B1 - A method for analyzing energy digestibility of feed - Google Patents

Abstract

The present invention relates to an equation for estimating the energy digestibility of piglet feed containing hatching by-products. By using the same, it is possible to simply estimate the energy digestibility of piglet feed using IVDMD or IVED of pig feed, and thus the composition of piglet feed is possible by simply and quickly predicting the digestibility.

Description

A METHOD FOR ANALYZING ENERGY DIGESTIBILITY OF FEED

The present invention relates to a method for analyzing energy digestibility in piglets, and specifically, to an equation capable of estimating the energy digestibility of hatchery byproducts in piglets.

Fish protein and plasma proteins, which are rich in protein and well digested, are frequently used in the feeding of weaned piglets. However, fishmeal and plasma proteins are expensive raw materials and fluctuate in price, resulting in an increase in feed costs (Keegan et al., 2004). Hatchery by-products, including unfertilized eggs, unfertilized eggs, culling and deadstock, eggshells, etc., are wastes regularly generated in hatcheries. It is known that there is a significant amount of protein in hatchery by-products, so there is a possibility to replace fish meal in pig feed (Dhaliwal et al., 1997).

Most animal feed is primarily aimed at supplying at least the minimum requirements for nutrients to life the animals that receive them. There is a need to analyze the feed for nutrient levels and determine when the feed meets the intended animal's energy and nutrient requirements. Nutrients in the animal feed become available to the animal by digestion of the feed in the animal's gastrointestinal tract. Digested nutrients are absorbed and used by animals for energy, growth, and development. Undigested nutrients pass through the intestines of most animals, reducing the nutritional value of the feed. In particular, the largest portion of feed for pig production. The raw material feed, which is the energy source, accounts for the largest portion of the feed cost. Therefore, it is important to measure the exact energy of the raw material feed. In addition, the digestibility test for nutrient evaluation is important in the sense of measuring the exact nutrient content when blending the raw material feed and seeing the optimal productivity of the animal with minimal feed cost.

In order to use a specific raw material feed in a blended feed, nutritional evaluation of the raw material feed must be preceded in order to prepare a precise blending ratio, but there have been no studies in the previous study that measured the available energy in hatchery byproducts in pigs.

An object of the present invention is to provide a method for analyzing the energy digestibility of hatchery byproducts in piglets.

In addition, it is an object of the present invention to provide an apparatus for measuring the energy digestibility of piglets in vitro by hatchery.

In addition, an object of the present invention is to provide a piglet feed containing hatching by-products.

In order to solve the above problems, the present invention provides a method for analyzing the energy digestibility of hatchery byproducts in piglets.

In addition, the present invention provides an apparatus for measuring energy digestibility in vitro from piglets.

In addition, the present invention provides a piglet feed containing hatching by-products.

According to the present invention, since the energy digestibility of hatchery by-products in piglets can be easily estimated by using IVDMD or IVED of piglets, there is an advantage in that piglets can be constructed by predicting digestibility easily and quickly.

1 is a photograph showing the drying process of hatching by-products.
2 is a view showing the correlation between in vivo energy digestibility and IVDMD (a) and IVED (b) in hatching by-products.
3 is a block diagram illustrating an apparatus for measuring the energy digestibility of hatchery byproducts in piglets.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings as an embodiment of the present invention. However, the following embodiments are presented as examples of the present invention, and when it is determined that a detailed description of a technique or configuration well known to those skilled in the art may unnecessarily obscure the subject matter of the present invention, the detailed description may be omitted. , Thereby, the present invention is not limited. The present invention is capable of various modifications and applications within the scope of the claims and the equivalents interpreted therefrom.

In addition, terms used in the present specification (terminology) are terms used to properly represent a preferred embodiment of the present invention, which may vary according to a user, an operator's intention, or customs in the field to which the present invention pertains. Therefore, definitions of these terms should be made based on the contents throughout the present specification. Throughout the specification, when a part “includes” a certain component, it means that the component may further include other components, not to exclude other components, unless otherwise stated.

All technical terms used in the present invention, unless defined otherwise, are used in the sense as commonly understood by those skilled in the art in the relevant field of the present invention. In addition, although a preferred method or sample is described herein, similar or equivalent ones are included in the scope of the present invention. The contents of all publications described by reference herein are incorporated into the present invention.

In one aspect, the present invention relates to a method for analyzing the energy digestibility of hatchery byproducts in piglets.

In one embodiment, the hatching by-products may be one or more selected from the group consisting of infertile eggs, unhatched eggs, culled chicks, and mixtures thereof, and pig feed using hatching by-products. Can be

In one embodiment, the energy digestibility of hatchery byproducts in piglets can be estimated using Equation 4 below, and IVDMD (in vitro dry matter disappearance) can be derived from Equation 1 below, DM _TI is the building mass, DM _UR is the mass of the undigested residue:

[Equation 4]

; 및

; And

[Equation 1]

.

.

In one embodiment, the energy digestibility of hatchery byproducts in piglets can be estimated using Equation 5 below, and IVED (in vitro energy disappearance) can be derived from Equation 2 below, and GE _TI Is the total energy of the test substance, and GE _UR is the total energy of the undigested residue:

[Equation 5]

; 및

; And

[Equation 2]

.

.

In one aspect, the present invention provides a building mass and a mass of undigested residues, or a total energy and total energy measurement of undigested residues 100; IVDMD or IVED operation unit 110; And it relates to an apparatus 200 for measuring the energy digestibility of hatchery by-products from piglets, including the in vitro energy digestibility calculation unit 120.

In one embodiment, IVDMD or IVED can be calculated by Equation 1 or Equation 2:

[Equation 1]

; 및

; And

[Equation 2]

.

.

In one embodiment, the in vitro energy digestibility can be calculated by Equation 4 or Equation 5 below:

[Equation 4]

; 및

; And

[Equation 5]

.

.

In one embodiment, the hatching by-products may be one or more selected from the group consisting of infertile eggs, unhatched eggs, culled chicks, and mixtures thereof, and pig feed using hatching by-products. Can be

In one aspect, the present invention relates to a piglet feed comprising hatching by-products.

In one embodiment, the hatched by-products may be one or more selected from the group consisting of unfertilized eggs, unfertilized eggs, culling shafts, and mixtures thereof, and it is more preferable that unfertilized eggs have high energy utilization.

The present invention will be described in more detail through the following examples. However, the following examples are only intended to materialize the contents of the present invention, and the present invention is not limited thereto.

Example 1. Hatchery by-product preparation

Infertile egg, unhatched egg and culled chick (Join Inc., Pyeongtaek, Republic of Korea) were crushed in 3 batches once per batch and dried at 130℃ for 20 hours. (DN 2300, Dongnam Tech Inc., Hwaseong, Republic of Korea) (Fig. 1). In addition, the mixture was prepared by mixing the dried unfertilized egg, unfertilized egg, and crustacean with 20%, 20%, and 60% (wt%), respectively, according to the moisture content and the natural occurrence rate in the hatchery.

Example 2. Chemical analysis of hatchery by-products

Each of the hatchery by-products (dried unfertilized eggs, unfertilized eggs and crustaceans) are each built (method 930.15; AOAC, 2005), crude protein (method 990.03; AOAC, 2005), ether extract (method 920.39; AOAC, 2005), ash ( Method 942.05; AOAC, 2005), calcium (method 978.02; AOAC, 2005) and phosphorus (method 946.6; AOAC, 2005) were analyzed (Table 1).

Example 3. Estimation of energy digestibility using IVDMD and IVED

3-1. Invitro building loss measurement

The IVDMD method consists of three steps that stimulate digestion of the stomach, small intestine, and large intestine based on Boisen & Fernandez (1997). Each of the hatchery by-products (dried unfertilized eggs, unfertilized eggs, crustaceans and mixtures thereof) were finely ground to pass through a 1-mm screen (Cyclotech 1093; Foss Tecator AB, Hoganas, Sweden) before the digestion process. In the first step, 0.5 g of sample was placed in a 125 mL conical flask, 25 mL of phosphate buffer (0.1 M and pH 6.0) and 10 mL of 0.2 M HCl were added to the flask. The pH of the solution in the flask was adjusted to 2.0 with 1 M HCl and NaOH solution and pepsin (10 mg/mL; ≥250 U/mg solids, P7000, pepsin from porcine gastric mucosa, Sigma-Aldrich, St. Louis, MO, USA ) Was added. Then 0.5 mL of chloramphenicol (C0378, Chloramphenicol, Sigma-Aldrich, St. Louis, MO, USA; 0.5 g/100 mL of ethanol) was added. The flask was stirred for 2 hours in a shake incubator at 39°C. In the second step, 10 mL of phosphate buffer (0.2 M and pH 6.8) and 5 mL of NaOH solution were added to the flask. The pH was then adjusted to 2.0 with 1M HCl and NaOH solution and 1 mL of pancreatin solution (50 mg/mL; 4×USP, P1750, pancreatin from porcine pancreas, Sigma-Aldrich, St. Louis, MO, USA) was added. . The flask was stirred for 4 hours in a shake incubator at 39°C. Then, 10 mL of 0.2 M EDTA solution was added and the pH was adjusted to 4.8 with 30% acetic acid and 1M NaOH solution. To the flask, 0.5 mL of Viscozyme (V2010, Viscozyme®L, Sigma-Aldrich, St. Louis, MO, USA) was added and incubated at 39° C. for 18 hours. After incubation, the undigested residue was filtered through a glass filter crucible containing 0.4 g of Celite using a Fibertec System (Fibertec System 1021 Cold Extractor, Tecator, Hgans, Sweden). The flask was rinsed twice with distilled water and then rinsed twice with 10 mL of 95% ethanol and 99.5% acetone. Thereafter, the glass filter crucible with the undigested residue was dried at 130° C. for 6 hours. The glass filter was cooled for 1 hour and then weighed. IVDMD was derived by the following equation (1).

DM _TI (weight of dry matter content in the test ingredient): the building mass of each hatchery byproduct; And

DM _UR (weight of dry matter content in the undigested residues): the mass of undigested residues.

As a result, the unfertilized egg, the unfertilized egg, the embryonic axis, and mixtures thereof were 81.7, 88.7, 83.9, and 85.4%, respectively, showing the highest IVDMD in the unfertilized egg (Table 2).

3-2. In Vitro Energy Loss Measurement

The IVED method was performed by slightly modifying the method of Regmi et al. (2008). After performing the three-step IVDMD method in the above embodiment, after collecting the undigested residue on the glass filter crucible, the total energy (gross energy) of the bomb calorimeter (Parr 1261 bomb calorimeter; Parr Instruments Co., Moline, IL, USA). In addition, the in vivo energy digestibility derived through animal experiments was calculated by Equation 3 below.

GE _TI (gross energy in the test ingredient): total energy of each hatchery byproduct; And

GE _UR (gross energy in the undigested residues): Total energy of undigested residues.

DE (digestible energy in the test ingredient): the plasticization energy of each hatchery by-product measured through animal testing; And

GE (digestible energy in the test ingredient): The total energy of each hatchery byproduct measured using a calorimeter.

As a result, the unfertilized egg, unfertilized egg, the embryonic axis, and mixtures thereof were 74.4, 85.1, 77.6, and 79.8%, respectively, showing the highest IVED in the unfertilized egg (Table 2).

3-3. In Vitro Energy Digestibility Estimation Equation

Since the in vivo and in vitro data were found to have a high positive correlation (r=0.83 and r=0.82) (FIG. 2), a prediction formula for in vivo energy digestibility of hatchery by-products using the REG procedure was obtained. It was developed based on in vitro analysis, and the in vitro energy digestibility derived from the two IVDMDs and IVEDs accurately predicted the in vivo energy digestibility derived from animal experiments (r ² =0.70 and 0.67, respectively).

According to the above embodiment, it can be seen that the energy use rate of un hatched eggs is the largest and is based on the high correlation between in vivo and in vitro data, so that the energy digestibility of hatchery by-products can be estimated through in vitro analysis data.

Claims (12)

Method of analyzing the energy digestibility of hatchery by-products in piglets, characterized by using the energy digestibility in vitro represented by the following equation (4)
[Equation 4]

[Equation 5]

(In the above equation 4 IVDMD (in vitro dry matter disappearance, in vitro building loss) is calculated by the following equation 1,
In Equation 5, IVED (in vitro energy disappearance) is calculated by Equation 2 below,
[Equation 1]

In Equation 1 above, DM _TI is the building mass, DM _UR is the mass of the undigested residue,
[Equation 2]

In Equation 2 above, GE _TI is the total energy of the test substance, GE _UR is the total energy of the undigested residue, DM _TI is the building mass, and DM _UR is the mass of the undigested residue). delete delete delete delete The method of claim 1, wherein the hatchery by-product is at least one selected from the group consisting of infertile eggs, unhatched eggs, culled chicks, and mixtures thereof. Building mass (DM _TI ) and mass of undigested residue (DM _UR ), or total energy of the test substance (GE _TI ) and total energy of undigested residue (GE _UR );
IVDMD operation unit represented by the following equation (1) or IVED operation unit represented by the following equation (2); And
Includes; in vitro energy digestibility calculation unit represented by the following equation (4) or (5),
In the case of using the IVDMD calculation unit, an in vitro energy digestibility calculation unit represented by Equation 4 below is used,
When using the IVED calculation unit, characterized in that using the in vitro energy digestibility calculation unit represented by the following equation (5),
Device for measuring the energy digestibility of hatchery byproducts in piglets:
[Equation 4]

[Equation 5]

(In the above equation 4 IVDMD (in vitro dry matter disappearance, in vitro building loss) is calculated by the following equation 1,
In Equation 5, IVED (in vitro energy disappearance) is calculated by Equation 2 below,
[Equation 1]

In Equation 1 above, DM _TI is the building mass, DM _UR is the mass of the undigested residue,
[Equation 2]

In Equation 2 above, GE _TI is the total energy of the test substance, GE _UR is the total energy of the undigested residue, DM _TI is the building mass, and DM _UR is the mass of the undigested residue). delete delete delete delete delete

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