KR102104257B1 - Manganese nanocolloid dispersion produced by hot melt extrusion method and uses thereof - Google Patents

Abstract

The present invention is a method for producing a manganese nanocolloidal dispersion comprising the step of heat-melting extrusion of a mixture of manganese, surfactants and solubilizers using a hot melt extruder, the manganese nanocolloidal dispersion prepared by the above method And a feed additive composition for enhancing manganese absorption and antioxidant activity containing the manganese nanocolloidal dispersion as an active ingredient.

Description

Manganese nanocolloid dispersion produced by hot melt extrusion method and use thereof {Manganese nanocolloid dispersion produced by hot melt extrusion method and uses thereof}

The present invention relates to a manganese nanocolloidal dispersion prepared using a hot melt extrusion method and uses thereof.

A micronutrient is a generic term for vitamins and minerals that are considered to be ingested in a small amount compared to nutrients ingested in large quantities, such as carbohydrate, fat, and protein, among nutrients present in the living body. Vitamins and vitamin analogs known as physiologically meaningful nutrients include vitamins B1, B2, B6, B12, A, D, K, C, E, niacin, pantothenic acid, biotin, Folic acid, choline, inositol, ubiquinone, carotene, etc., and inorganic substances include calcium (Ca), phosphorus (P), magnesium (Mg), and sodium (Na), potassium (K), chlorine (Cl), iron (Fe), copper (Cu), zinc (Zn), manganese (Mn), iodine (I), cobalt (Co), molybdenum (Mo), selenium (Se), chromium (Cr), and the like. Micronutrients are often present in trace amounts, but are essential for improving animal survival, growth, health and fertility.

Manganese is an essential component for carbohydrate, fat and protein metabolism, reproductive function, and cartilage development, and is abundant in bone, liver, muscle, and skin. In the case of feeding by adding manganese to livestock feed, the absorption rate of manganese is low, so most of it is excreted through the manure, and manganese contained in the manure inhibits the growth of microorganisms to decompose the manure and decompose the manure. Delays can also increase soil pollution. Pig farms prefer manganese additives with high absorption to prevent animal manganese deficiency, and accordingly, studies on manganese additives that have high manganese utilization in livestock and can reduce environmental problems are needed.

On the other hand, Korean Registered Patent No.1753222 discloses 'Danggui-solid dispersion prepared by melt extrusion and its manufacturing method', and Korean Registered Patent No. 1390946 discloses limaprost, a pharmaceutically active ingredient, as tamsulo. The 'manufacturing method for low-content pharmaceutical compositions using high-temperature melt extrusion' of Shins (Tamsulosin) and Glimepiride has been disclosed, but the manganese nanocolloidal dispersions prepared using the hot-melt extrusion method of the present invention and its use No description has been made.

The present invention has been derived by the above-mentioned demands, in the present invention broiler feed fed with manganese-free basal diet and manganese-free manganese (organic manganese or organic manganese) treated Alternatively, compared to weaned pigs, the present invention is confirmed by improving the absorption effect of manganese in the broiler or weaned pigs fed with a feed treated with a manganese nanocolloidal dispersion prepared using a heat-melting extrusion method, and promoting the antioxidant activity. Completed.

In order to solve the above problems, the present invention provides a method of manufacturing a manganese nanocolloidal dispersion comprising extruding a mixture of manganese, a surfactant, and a solubilizer using a hot melt extruder.

In addition, the present invention provides a manganese nanocolloidal dispersion prepared by the above method.

In addition, the present invention provides a feed additive composition for enhancing manganese absorption and antioxidant activity containing the manganese nanocolloidal dispersion as an active ingredient.

The manganese nanocolloidal dispersion of the present invention has excellent efficiency as a feed additive and can improve the productivity of animals, and the particle size is significantly reduced compared to manganese that has not undergone thermal melt extrusion, thereby increasing the absorption rate in the body and causing environmental pollution. It is expected to bring about the effect of preventing environmental pollution by suppressing emissions.

1 is a particle size distribution (A) and transmission electron micrograph (B) of manganese nanocolloidal dispersion (HME-Mn) particles prepared using a hot melt extrusion method dispersed in distilled water.
2 is a result of analyzing the constituent elements present on the particle surfaces of HME-Mn particles (B) and MnSO ₄ particles (A) through X-ray photoelectron spectroscopy.
3 is a result of analyzing crystal structure changes of HME-Mn particles and MnSO ₄ particles through X-ray diffractometry.
4 is a result of analyzing the interaction between the chemical functional groups of the surfactant and the solubilizing agent and MnSO ₄ through a Fourier-transform infrared spectrometry method.

In order to achieve the object of the present invention, the present invention is a manganese nanocolloidal dispersion comprising the step of hot melt extrusion using a hot melt extruder of a mixture of manganese, surfactants and solubilizers (hereinafter , HME-Mn).

In the method according to an embodiment of the present invention, the surfactant may be Span 80 (sorbitan monooleate) and Tween 80 (polyoxyethylene sorbitan monooleate), and the solubilizing agent is PEG (polyethylene glycol) 6000, PEG 400, PEG 1000 , PEG 8000, PEG 20000 or Soluplus (polyvinyl capralactam-polyvinyl acetate-polyethylene glycol graft copolymer), preferably PEG 6000, but is not limited thereto.

Span 80 and Tween 80 according to the present invention are O / W type emulsifiers, which can coexist with anionic and cationic emulsifiers, and were used as nonionic surfactants that increase the action.

PEG 6000 according to the present invention was used as a solubilizer that serves to distribute the lipophilic component evenly in a formulation made of a hydrophilic component.

In the method according to one embodiment of the present invention, the manganese, surfactant and solubilizer may be mixed in a weight ratio of 18 to 22:15 to 17:62 to 66, preferably 20:16 based on the total weight It can be mixed in a weight ratio of: 64, but is not limited thereto.

In the method according to an embodiment of the present invention, the mixture is mixed with manganese sulfate (MnSO ₄ ), Span 80, Tween 80 and PEG 6000 in a weight ratio of 18 to 22:10 to 14: 3 to 5:62 to 66 It may be, preferably, manganese sulfate, Span 80, Tween 80 and PEG 6000 may be mixed in a weight ratio of 20: 12: 4: 64, but is not limited thereto.

In the method according to the embodiment of the present invention, the mixture may further include an ionizing agent, and the ionizing agent may be acetic acid or omija, but is not limited thereto. The Omija means, but is not limited to, Omija powder, Omija juice or Omija extract.

In the method according to an embodiment of the present invention, in the heat-melting extrusion, the temperatures of the barrel and the die are maintained at 50-60 ° C and 40-50 ° C, respectively, and the screw speed is 130-180 rpm, and the extrusion speed is It may be performed at 40-50 g / min, preferably, the temperatures of the barrel and the die are maintained at 55 ° C and 45 ° C, respectively, the speed of the screw is 150 rpm, and the extrusion speed is performed at 45 g / min. It may be, but is not limited thereto.

In the method according to an embodiment of the present invention, the heat-melting extruder includes a double screw, and preferably includes an extrusion die having a diameter of 0.8 to 1.2 mm. Compared to single screw extruders, double screw extruders offer the following advantages: high dispersibility, easy material supply, low risk of overheating, high process productivity and effective adjustment of process parameters. The strong force generated through the double screw system as described above can uniformly disperse manganese in a PEG 6000 base, and at the same time, the nano-sized particles of manganese are covered with organic polymers (eg, PEG 6000), and on the manganese particles. The PEG 6000 coating reduces surface energy and aggregate generation.

The method for manufacturing HME-Mn according to an embodiment of the present invention is specifically, manganese sulfate, Span 80, Tween 80 and PEG 6000 in a weight ratio of 18 to 22:10 to 14: 3 to 5:62 to 66 The mixed mixture, using a hot melt extruder comprising a double screw and an extrusion die diameter of 0.8 to 1.2 mm, the temperatures of the barrel and die are 50 to 60 ° C and 40 to 50 ° C, respectively, and the screw speed is 130 to 180 rpm, and the extrusion speed may be produced by heat-melting extrusion at 40-50 g / min, and more specifically, manganese sulfate, Span 80, Tween 80 and PEG 6000 are mixed in a weight ratio of 20: 12: 4: 64. Mixture, the temperature of the barrel and die is 55 ° C. and 45 ° C., the screw speed is 150 rpm, and the extrusion speed is 45 g / min. It may be manufactured by heat-melting extrusion in min, but is not limited thereto.

In addition, the present invention provides a manganese nanocolloidal dispersion prepared by the above method.

In the manganese nanocolloidal dispersion according to an embodiment of the present invention, the size of the manganese nanocolloidal dispersion may be 50 to 500 nm, preferably 169 nm, but is not limited thereto. It is possible to control the size of the dispersion as much as possible.

In addition, the present invention provides a feed additive composition for enhancing manganese absorption and antioxidant activity containing the manganese nanocolloidal dispersion as an active ingredient. The feed additive composition of the present invention contains a manganese nanocolloidal dispersion prepared by heat-melting extrusion of a mixture of manganese, surfactant, and solubilizer as an active ingredient using a hot-melt extruder, and is added to feed to feed If possible, it is possible to improve the manganese absorption rate and antioxidant activity of the target animal.

The target livestock according to the present invention may be pig, chicken, cow, sheep, etc., preferably pig or chicken, but is not limited thereto.

The feed additive composition of the present invention may be administered alone to livestock, including pigs, chickens, cattle, etc., or in combination with other feed additives in an edible carrier. In addition, the feed additive composition can be easily administered as a top dressing or by mixing them directly into livestock feed or separately from the feed, in a separate oral dosage form, or in combination with other ingredients. Typically, a single daily intake or a divided daily intake may be used, as is well known in the art.

The feed additive composition of the present invention corresponds to the supplementary feed under the feed management method.

The feed additive composition of the present invention may be added to the feed, and "feed" means any natural or artificial diet, one meal or the like, or one component of the meal, for the animal to eat, eat, and digest. can do. The type of the feed is not particularly limited, and a feed commonly used in the art may be used. Non-limiting examples of the feed, vegetable feed, such as grains, muscles, food processing by-products, algae, fiber, pharmaceutical by-products, fats and oils, starches, peels or grain by-products; And animal feeds such as proteins, inorganics, fats and oils, minerals, fats and oils, single cell proteins, animal planktons and food.

In one embodiment of the present invention, the feed additive composition may be 1.5 to 4.5% by weight, preferably 3% by weight, based on the feed composition, but is not limited now.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, the content of the present invention is not limited to the following examples.

Materials and methods

1. Reagent

Manganese sulfate (MnSO ₄ ) is TMC (TMC Co., Ltd., Korea), PEG 6000 is Samchun Pure Chemical Co., Ltd. (Korea), Span 80 and Tween 80 are Daejung Chemicals (Daejung Chemical) & Metals Co., Ltd., Korea).

2. Analysis of particle properties of manganese nanocolloidal dispersion

Particle properties of the manganese nanocolloidal dispersion (hereinafter HME-Mn) include hydrodynamic size, polydispersity index and zeta potential, and dynamic light scattering (DLS). It was analyzed according to the manufacturer's instructions using a laser Doppler method (ELS-Z1000; Otsuka Electronics, Japan). Further, in order to analyze the shape of the HME-Mn particles, HME-Mn was placed on a copper grid containing a film, dried for 10 minutes, and then observed with a transmission electron microscopy (TEM; JEM 1010, Japan). The HME-Mn used in the experiment was dispersed in distilled water and diluted to a concentration of 20 mg / ml.

3. Solid state analysis

X-ray photoelectron spectroscopy (XPS); K-AlphaTM +, to analyze elements present in the thin layer of the particle surface of HME-Mn and manganese sulfate (hereinafter referred to as MnSO ₄ ) that have not undergone thermal melt extrusion Thermo Fisher Scientific, UK). In addition, HME-Mn and MnSO ₄ Crystalline structure changes were analyzed by X-ray diffractometry (XRD; Philips X 'Pert PRO MPD diffractometer, PANalytical Corp., The Netherlands). The generator voltage and tube current were set to 40 kV and 30 mA, respectively, and the step scan time and step size were set to 8.67 seconds and 0.013 degrees, respectively. Changes in the chemical functions of HME-Mn and MnSO ₄ were analyzed using Fourier-transform infrared spectrometry (FT-IR). The wave number dependent on the transmittance (%) value was measured by the ATR (Attenuated total reflectance) mode of a FT-IR spectrometer (PerkinElmer Inc., UK), and the transmittance (%) value was 400 to 4,000 cm ^-1 wave number Monitored in scope.

4. Animal Experiment-Broiler

4-1. Animals, feed and feeding

A total of 800 broilers (Ross 308) were divided into 8 treatment groups according to their weight, and each treatment was divided into 5 repetitions of 20 treatments. Each of the eight treatments is 1) broiler fed a basic feed that has not been treated with anything, 2) broiler fed a feed treated with 60 ppm inorganic manganese (Morgan), 3) inorganic with a concentration of 120 ppm Broilers fed with manganese-treated feed, 4) Broilers fed with 200 ppm inorganic manganese-fed, 5) Broilers fed with 60 ppm HME-Mn-treated feed, 6) Broilers fed feed treated with HME-Mn at a concentration of 120 ppm, 7) Broilers fed feed treated with HME-Mn at a concentration of 200 ppm, and 8) Organic manganese at 120 ppm concentration (organic-Mn) The treated feed was divided into broiler broilers. Experimental feed was fed by dividing feed prepared at different mixing ratios into stages (1 stage; 1-14 days, 2 stages; 15-35 days) (Table 1).

4-2. Specification Performance Survey

Broiler weights were measured individually at the start of the experiment and at the end of each step (Days 14 and 35). The weight of unconsumed feed was measured at the end of the experiment, and the feed intake was measured at the end of each step. Based on the measured weight gain and feed intake, feed conversion ratio (FCR) was analyzed.

4-3. Manganese absorption rate measurement

Feed according to the method of AOAC (Official Methods of Analysis of the Association of Official Analytical Chemists International. 18th ed.Gaithersburg, MD, USA) using high-frequency inductively coupled plasma (ICP) emission spectroscopy The absorption rate of manganese was analyzed by measuring the amount of manganese in feces, serum, shin bone and liver.

First, the manganese content in the feed and feces was placed in a muffle furnace at 600 ° C. for 4 hours in a muffle furnace and cooled to dry ash, cooled, and then added to 10 ml of 50% HCl (v / v). Dissolved. The dissolved sample was left overnight with the lid closed, filtered 2-3 times with a Whatman filter in a 100 ml flask, diluted with deionized distilled water, and measured.

In addition, the content of manganese in plasma is 1 ml of blood sample placed in a porcelain crucibles, dried in an oven at 105 ° C. for 4 hours, placed in a muffle furnace at 600 ° C. for 1 hour, cooled, and the same as above. It was measured by the method. In addition, in order to analyze the manganese content in the liver, the liver sample was dried and ground at 105 ° C. for 24 hours. 1 g of the liver sample powder was placed in a muffle furnace at 600 ° C. for 1 hour to make dried ash, cooled, and then measured in the same manner as above.

In addition, the manganese content in the shin bone was removed from the fat and tissue attached to the shin bone, and then dried and crushed the shin bone at 105 ° C for 24 hours. 0.5 g of the shin bone sample powder was placed in a muffle furnace at 600 ° C. for 1 hour to make dried ash, cooled, and then measured in the same manner as above.

4-4. Analysis of blood composition changes

After selecting 10 animals from each treatment broiler, blood is collected at the end of each stage, and red blood cells (RBC) and white blood cells (WBC) are analyzed using a Natt-Herrick solution for hemocytometer ( Hemocytometer was measured, and haematocrit (HCT) and hemoglobin were measured using microhematocrit and cyanmethemoglobin, respectively.

4-5. Chest muscle analysis

The pH of the chest muscle was homogenized (homogenizer; PH91, SMT Co., Ltd., Japan) by homogenizing the chest muscle for 1 minute at 10,000 rpm, followed by a pH meter (Seven Easy pH; Mettler-Toledo GmbH, Switzerland) ). The muscle color of the chest muscle was analyzed according to the CIE (Commission International de l'Eclairage) color value using a color meter (CR-400, Konica Minolta Sensing Inc., Japan), and the muscle of the ventral part of the right breast was used. To measure the average of three colors (lightness, redness, yellowness). The rate of loss of gravy in the chest muscle is the percentage of the initial muscle weight by excising the chest muscle, measuring the weight, placing it in a polyethylene bag, storing it at 4 ° C for 24 hours, then removing the chest muscle, wiping it and weighing it. It is represented by. The fat content in the chest muscle was 1 g of the chest muscle, and was performed according to AOAC (Association of Official Analytical Chemists, 1990) 960.39 method.

4-6. Antioxidant activity measurement

Three drops of antioxidant solution (3% BHA, 54% propylene glycol, 3% BHT, 40% Tween 20) was added to 0.5 g of crushed chicken leg sample, and thiobarbituric acid (1% 4,6-Dihydroxy-2) 3 ml of -mercaptopyrimidine) solution and 17 ml of 25% trichloroacetic acid were mixed and then reacted for 30 minutes in a constant temperature water bath set at 100 ° C and cooled for another 30 minutes. The cooled mixture was centrifuged at 2,400 x g for 30 minutes to obtain a supernatant, and absorbance was measured at 532 nm with an absorbance meter (UV-mini-1240, Japan).

4-7. Statistical analysis

Statistical analysis of all data obtained in the experiment was performed through the General Linear Model (GLM) process of the SAS (Statistical Analysis System) program. Significant differences between treatment means were divided into Tukey's Honestly Significant Difference test. The repeat sugar of broiler was used as an experimental unit for specification analysis, and was judged to be statistically significant when the p value was less than 0.05.

5. Animal Experiment-Weaning pig

5-1. Animals, feed and feeding

A total of 150 weaned piglets (Yorkshire × Landrace × Duroc) were divided into 6 treatment groups according to the initial average weight (6.61 ± 0.26 kg), and each treatment was further divided into 6 replicates per 6 replicates. Each of the six treatment groups was 1) weaned pigs fed a basic feed with no treatment, 2) weaned pigs fed a 20 ppm concentration of inorganic feed, and 3) 40 ppm inorganic manganese. Weaned piglets fed the treated diet, 4) Manganese nanocolloidal dispersion fed with a concentration of 20 ppm (HME-Mn), Weaned piglets fed with treated feedstock, 5) Manganese nanocolloidal dispersion with a concentration of 40 ppm (HME-Mn) ) Treated feeds were divided into weaned piglets and 6) 40 ppm concentration of organic manganese fed feeds were fed into weaned piglets. Experimental feeds were fed by dividing feeds prepared at different mixing ratios into stages (1 stage; 0-14 days, 2 stages; 15-28 days) (Table 2).

5-2. Specification Performance Survey

The weight of weaned piglets was measured individually at the start of the experiment and at the end of each stage (Days 14 and 28), and feed consumption was measured at the end of each stage. Based on the measured body weight and feed consumption, the average daily gain (ADG), the average daily feed intake (ADFI), and the feed efficiency (gain to feed ratio, G: F) were analyzed.

5-3. Analysis of blood composition changes

After randomly selecting and sacrificed one animal per repeat per treatment group, 10 ml of blood was collected from jugular vein using a disposable vacuum tube that does not contain an anticoagulant. The collected blood samples were centrifuged for 15 minutes at 3,000 xg at 4 ° C, and serum was separated and stored at 4 ° C.

5-4. Manganese absorption rate measurement

The content of manganese in feed, feces, liver and plasma of weaned piglets was measured and analyzed in the same manner as the method for measuring the absorption rate of manganese in broilers described in 4-3 above.

5-5. Analysis of blood composition changes

The blood of weaned piglets was collected at the end of each step and the red blood cell, white blood cell and hemoglobin content and red blood cell volume (haematocrit) were measured through an automated hematologic analysis.

5-6. Statistical analysis

Statistical analysis of all the data obtained in the experiment was performed in the same manner as described in 4-7 above, the repeat sugar of weaned piglets was used as an experimental unit for specification performance, and weaned piglets were used as an experimental unit for manganese absorption analysis. When the p value was less than 0.05, it was judged to be statistically significant.

Preparation Example 1 Preparation of manganese nanocolloidal dispersion

Manganese sulfate (MnSO ₄ ), Span 80, Tween 80 and PEG 6000 are mixed immediately before extrusion in a weight ratio of 20: 12: 4: 64, and a double screw hot melt extruder having a circular injection port (1.0 mm diameter) (STS-25HS) , Hankook EM Ltd., Pyoungtaek, Korea) and extruded at a rate of 45 g / min to prepare a manganese nanocolloidal dispersion (HME-Mn), the temperature of the barrel and the die being 55 ° C and 45 ° C, respectively. The speed of was set at 150 rpm. The mixture was extruded in a die after passing through a conveying and kneading process in a barrel, and the extrudate was cooled at room temperature and then pulverized with a grinder (HBL-3500S, Samyang Electronics Co., Korea).

Example 1. Particle characterization of manganese nanocolloidal dispersion

The manganese sulfate powder itself is in microns (micron, μm) and does not dissolve well in water, making it difficult to absorb from the gastrointestinal mucosal epithelium when administered orally. Therefore, by dispersing the manganese sulfate in the form of colloidal particles in an aqueous solution using a hot melt extrusion method, the particle diameter was reduced in nano units to increase the absorption rate in the gastrointestinal tract. During the hot melt extrusion process, manganese sulfate was dispersed in a surfactant and a solubilizer and the surface was coated to reduce the surface tension.

As a result of analyzing the HME-Mn particles prepared in Preparation Example 1, the average diameter was 169 nm, the particle size distribution was observed in a single peak shape, and the average value of the zeta potential was -11 mV (FIG. 1A). In addition, as a result of observing HME-Mn particles with a transmission electron microscope, it was confirmed that spherical nanoparticles corresponding to the size of HME-Mn were generated (FIG. 1B). Through this , it was found that when HME-Mn was dispersed in an aqueous environment, it had a relatively uniform particle size distribution without aggregation of particles.

Example 2. Solid state analysis

As a result of analyzing the elements present on the particle surface of HME-Mn and MnSO ₄ through X-ray photoelectron spectroscopy, the content of Mn, S and O is HME compared to MnSO ₄ particles (8.48%, 13.28%, 57.63% respectively). -Mn particles (0.61%, 0.53%, 19.56% respectively) decreased, the content of C was significantly increased in HME-Mn (79.29%) than MnSO ₄ (19.09%) (Fig. 2). Through this, it is considered that the organic substances surfactant (Span 80, Tween 80) and solubilizer (PEG 6000) surround the surface of the MnSO ₄ powder particles and the element content near the surface is changed.

In addition, as a result of analyzing the crystal structure change pattern of the HME-Mn and MnSO ₄ particles through X-ray diffraction analysis, it was confirmed that the MnSO ₄ particles have a crystalline structure, and the HME-Mn particles have characteristic intensity peaks exhibited by the MnSO ₄ particles. It was observed, and peaks related to surfactant and solubilizer were also observed at different angles (FIG. 3). Through this, it was found that the HME-Mn particles maintain the crystalline structure of MnSO ₄ well and also maintain the crystalline structure of the surfactant and solubilizer.

In addition, as a result of analyzing the interaction between the chemical functional groups of the surfactant and the solubilizer and MnSO ₄ through a Fourier transform infrared spectroscopy method, MnSO ₄ particles are 501 cm ^-1 , 605 cm ^-1 , 813 cm ^-1 and 1090 cm It woke the percent transmittance decreases symptoms appear at ^-1, HME-Mn particles is ^{509 cm -1, 603 cm -1,} 841 cm -1 and 1096 cm ^-1 as well as the ^{1341 cm -1, 1740 cm -1,} 1966 cm - ^{It was} also confirmed that the percent transmittance was decreased in ¹ (FIG. 4). Through the above results, it was found that MnSO ₄ was efficiently dispersed in a surfactant and a solubilizing agent in a heat-melting extrusion process to be prepared as HME-Mn.

Example 3. Broiler experiment results

3-1. Specification

The specifications of broilers fed with feed treated with HME-Mn (60, 120 and 200 ppm), inorganic manganese (60, 120 and 200 ppm) or organic manganese (120 ppm) were investigated.

As a result, the feed demand rate calculated by dividing the weight gain and the feed intake in the daily weight increase in the inorganic manganese, organic manganese, and HME-Mn treatment groups, compared to the control that ate the basic feed that did not process anything, (FCR) decreased (Table 3).

3-2. Analysis of blood composition changes

Blood from broilers fed with feed treated with HME-Mn (60, 120 and 200 ppm), inorganic manganese (60, 120 and 200 ppm) or organic manganese (120 ppm) was analyzed.

As a result, it was confirmed that the content of white blood cells (WBC), red blood cells (RBC), and hemoglobin (Hb) and red blood cell volume (HCT) were not significantly different between the control and manganese treatment groups that consumed the basic feed treated with nothing (( Table 4). Through this, it is thought that ingesting manganese regardless of the formulation will not affect the blood composition and immune response of broilers.

3-3. Antioxidant activity assay

Absorbance values were measured for concentrations of thiobarbituric acid reactive substance (TBARS) in broilers fed with HME-Mn (120 ppm), inorganic manganese (120 ppm) or organic manganese (120 ppm) treated feed. TBARS is produced by reacting malonaldehyde (thiobarbituric acid) with TBA (thiobarbituric acid), which is produced by peroxidation of various biofilms, and analyzed the effect of antioxidant activity through the inhibition rate of TBARS production.

As a result, the absorbance value was decreased in the HME-Mn treatment group compared to the control group that consumed the basic feed that was not treated with anything and the inorganic or organic manganese treatment group, and the absorbance value was significantly decreased on the 9th day of ingestion (Table 5). .

3-4. Manganese absorption rate analysis

HME-Mn (60, 120 and 200 ppm), inorganic manganese (60, 120 and 200 ppm) or organic manganese (120 ppm) treated feeder broiler feces, tibia, The absorption rate of manganese in liver, plasma and feed was analyzed.

As a result, the manganese content in the feces was significantly reduced in the HME-Mn treatment group compared to the inorganic manganese or organic manganese treatment group, which means that the HME-Mn ingested through the feed was well absorbed into the broiler's body. The content of manganese in plasma, liver and shin bone increased in HME-Mn treatment compared to inorganic manganese or organic manganese treatment, and through this, HME-Mn prepared using heat-melting extrusion method can improve the absorption rate in animals. Was found (Table 6).

3-5. Chest muscle analysis

HME-Mn (60, 120 and 200 ppm), inorganic manganese (60, 120 and 200 ppm), or organic manganese (120 ppm) treated with feed, pH, water content (moisture) for the chest muscles of broilers , Drip loss, intramuscular fat and meat color (ligntness, redness, yellowness) were measured.

As a result, it was confirmed that there was no significant difference between the control group and the manganese treatment group, which consumed the basic feed that did not process anything, such as the pH, moisture content, meat loss rate, and color of the chest muscle, and the basis fat content was not treated with the basic feed. Compared to the control intake of, it was significantly decreased in inorganic manganese, organic manganese and HME-Mn treatment (Table 7).

In addition, as a result of analyzing the content of dressing percentage and breast muscle, thigh muscle and abdominal fat, the results of the cognitive yield and the content of the chest muscle and thigh were processed. There was no significant difference between the control group and the manganese treatment group that did not consume the basic feed, and it was confirmed that the abdominal fat content was decreased in the manganese treatment group compared to the control group that consumed the basic feed, especially HME-Mn compared to the inorganic manganese treatment group. A further decrease was observed in the organo-manganese treatment (Table 8). Through this, it was found that when feeding the feed supplemented with HME-Mn, the meat quality of broilers could be improved.

Example 4. Results of weaning piglets

4-1. Specification

The specifications of weaned piglets fed with diets treated with HME-Mn (20 and 40 ppm), inorganic manganese (20 and 40 ppm) or organic manganese (40 ppm) were investigated.

As a result, the daily gain (ADG) was significantly increased in the HME-Mn treatment group compared to the control and inorganic manganese or organic manganese treatment groups that ate the basic feed that was not treated with anything, and the daily weight gain was increased ( Feed intake) was calculated by dividing feed efficiency (G: F) in the HME-Mn treated group compared to the control group, but there was no significant difference (Table 9). Through this, HME-Mn was thought to be able to improve the productivity of animals when feeding to animals treated.

4-2. Analysis of blood composition changes

The blood of weaned piglets fed a diet treated with HME-Mn (20 and 40 ppm), inorganic manganese (20 and 40 ppm) or organic manganese (40 ppm) was analyzed.

As a result, the content of white blood cells (WBC), red blood cells (RBC), and hemoglobin (Hb) and red blood cell volume (HCT) were found to have no significant difference between the control and manganese treatment groups that consumed the basic feed treated with nothing (( Table 10). Through this, it was judged that ingesting manganese regardless of the formulation did not affect the blood composition and immune response of weaned piglets.

4-3. Manganese absorption rate analysis

Feces and plasma and feed of weaned piglets fed feed treated with HME-Mn (20 and 40 ppm), inorganic manganese (20 and 40 ppm) or organic manganese (40 ppm). Manganese absorption was analyzed.

As a result, the manganese content in the feces was significantly reduced in the HME-Mn treatment group compared to the inorganic manganese or organic manganese treatment group, which means that the HME-Mn ingested through the feed was well absorbed into the broiler's body. In addition, the plasma and liver manganese content was increased in the HME-Mn treatment group compared to the inorganic manganese or organic manganese treatment group. Through this, it was found that the HME-Mn prepared using the hot melt extrusion method can improve the absorption rate in the body of the animal. (Table 11).

Claims (13)

A mixture of manganese sulfate (MnSO ₄ ), Span 80, Tween 80 and PEG 6000 in a weight ratio of 18 to 22:10 to 14: 3 to 5:62 to 66, including a double screw, and an extrusion die diameter of 0.8 to The temperature of the barrel and die is 50-60 ° C and 40-50 ° C, respectively, using a 1.2 mm heat-melting extruder, the screw speed is 130-180 rpm, and the extrusion speed is 40-50 g / min. Method for producing a manganese nanocolloidal dispersion comprising the step of. delete delete delete delete delete delete delete delete delete A manganese nanocolloidal dispersion having a size of 50-500 nm prepared by the method of claim 1. delete A feed additive composition for enhancing manganese absorption and antioxidant activity, comprising the manganese nanocolloidal dispersion of claim 11 as an active ingredient.

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