KR102085242B1 - 표적 핵산 검출을 위한 방법 및 조성물 - Google Patents

표적 핵산 검출을 위한 방법 및 조성물 Download PDF

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KR102085242B1
KR102085242B1 KR1020137033544A KR20137033544A KR102085242B1 KR 102085242 B1 KR102085242 B1 KR 102085242B1 KR 1020137033544 A KR1020137033544 A KR 1020137033544A KR 20137033544 A KR20137033544 A KR 20137033544A KR 102085242 B1 KR102085242 B1 KR 102085242B1
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로버트 테르브루겐
옌보우 리우
존 레이 칠더스
김창희
마지드 알. 아베디
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디엑스테리티 다이아그노스틱스 인코포레이티드
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KR1020137033544A 2011-05-17 2012-05-17 표적 핵산 검출을 위한 방법 및 조성물 Active KR102085242B1 (ko)

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
WO2023182742A1 (ko) * 2022-03-24 2023-09-28 전북대학교산학협력단 역전사를 사용하지 않는 결찰 방법을 이용한 cdna 합성 기반 표적 유전자 검출용 조성물 및 다중 결찰 보조 재조합효소 중합효소 증폭 방법

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2677039B8 (en) * 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
KR102085242B1 (ko) * 2011-05-17 2020-03-05 디엑스테리티 다이아그노스틱스 인코포레이티드 표적 핵산 검출을 위한 방법 및 조성물
US9777322B2 (en) 2011-07-08 2017-10-03 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
DK3511423T4 (da) 2012-10-17 2024-07-29 Spatial Transcriptomics Ab Fremgangsmåder og produkt til optimering af lokaliseret eller rumlig detektion af genekspression i en vævsprøve
US9080167B2 (en) * 2012-11-16 2015-07-14 Covaris, Inc. System and method for processing paraffin embedded samples
US9624533B2 (en) 2012-12-07 2017-04-18 Invitae Corporation Multiplex nucleic acid detection methods
KR101829668B1 (ko) * 2013-03-27 2018-02-14 김성천 생체분자와 단일가닥핵산의 결합정보를 생성하기 위한 기준물질 및 핵산칩, 이들의 제조방법 및 이들을 이용한 생체분자 분석방법 및 장치
US10510435B2 (en) 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
CN110669826B (zh) 2013-04-30 2025-01-07 加州理工学院 通过顺序杂交编条形码的分子多重标记
EP4234716A3 (en) 2013-06-25 2023-12-06 Prognosys Biosciences, Inc. Methods for determining spatial patterns of biological targets in a sample
US9757095B2 (en) 2014-06-10 2017-09-12 Dxterity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
US10870845B2 (en) 2014-07-01 2020-12-22 Global Life Sciences Solutions Operations UK Ltd Methods for capturing nucleic acids
US11091810B2 (en) * 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
US9856521B2 (en) * 2015-01-27 2018-01-02 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US10683534B2 (en) 2015-01-27 2020-06-16 BioSpyder Technologies, Inc. Ligation assays in liquid phase
CN107002147B (zh) * 2014-12-11 2022-01-28 环球生命科学解决方案运营英国有限公司 用于俘获核酸的方法
CN105803055A (zh) * 2014-12-31 2016-07-27 天昊生物医药科技(苏州)有限公司 一种基于多重循环延伸连接的靶基因区域富集新方法
EP4119677B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
SG10202107055SA (en) * 2015-07-17 2021-08-30 Nanostring Technologies Inc Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
EP3325669B1 (en) * 2015-07-24 2023-07-05 The Johns Hopkins University Compositions and methods of rna analysis
WO2017027627A1 (en) * 2015-08-10 2017-02-16 Life Technologies Corporation Biological sample preparation for testing
US12421540B2 (en) 2016-08-01 2025-09-23 California Institute Of Technology Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism
US10451599B2 (en) 2016-09-02 2019-10-22 Zepto Life Technology, LLC Methods of isolating and detecting metal ions from proteins
WO2019046768A1 (en) * 2017-08-31 2019-03-07 William Marsh Rice University SYMBOLIC SEQUENCING OF DNA AND RNA BY SEQUENCE CODING
CN120210336A (zh) 2017-10-06 2025-06-27 10X基因组学有限公司 Rna模板化连接
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US20220042090A1 (en) * 2018-09-14 2022-02-10 Cold Spring Harbor Laboratory PROGRAMMABLE RNA-TEMPLATED SEQUENCING BY LIGATION (rSBL)
US20220290242A1 (en) * 2018-11-05 2022-09-15 Centre Henri Becquerel Method for diagnosing a cancer and associated kit
US12385083B2 (en) 2018-12-10 2025-08-12 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3925703B1 (en) * 2019-02-14 2025-04-16 Korea University Research and Business Foundation Method for extracting microvesicles from biological samples
EP3998349A3 (en) * 2019-03-01 2022-08-24 Mercy Bioanalytics, Inc. Compositions and methods for target protein detection on exosomes using proximal ligation
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2021030271A2 (en) 2019-08-09 2021-02-18 Nutcracker Therapeutics, Inc. Methods and apparatuses for manufacturing for removing material from a therapeutic composition
WO2021050560A1 (en) * 2019-09-09 2021-03-18 Sherlock Biosciences System
US20210108259A1 (en) * 2019-10-10 2021-04-15 Liquid Biopsy Research LLC Compositions, methods and kits for biological sample and rna stabilization
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
JP2023503349A (ja) * 2019-11-26 2023-01-27 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 標的の分析に関する組成物、セット、および方法
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
WO2021133849A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4549582A3 (en) 2020-04-22 2025-06-18 10x Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4553168A3 (en) 2020-05-22 2025-08-27 10x Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2981265T3 (es) 2020-06-08 2024-10-08 10X Genomics Inc Métodos para determinar un margen quirúrgico y métodos de uso del mismo
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
CN111944875A (zh) * 2020-06-28 2020-11-17 温州医科大学附属眼视光医院 一种防止rna降解的保护方法、保护序列、组合物和试剂盒及其应用
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
AU2021358061A1 (en) * 2020-10-06 2023-06-08 Materials and Machines Corporation of America Method for detection of rna or dna from biological samples
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4060049B1 (en) * 2021-03-18 2023-08-23 Genomill Health Oy Methods for accurate parallel quantification of nucleic acids in dilute or non-purified samples
WO2022221425A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CA3224264A1 (en) * 2022-04-08 2023-10-12 Illumina, Inc. Aptamer dynamic range compression and detection techniques
WO2024080776A1 (ko) * 2022-10-14 2024-04-18 고려대학교 산학협력단 비색 검출을 위한 황 함유 프로브 및 핵산 내 황 도입을 통한 효소 활성 가속화 방법
WO2025168224A1 (en) * 2024-02-08 2025-08-14 Leica Microsystems Cms Gmbh Marker and method for analysing a biological sample

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010114599A1 (en) * 2009-04-01 2010-10-07 Dx Terity Diagnostics Inc. Chemical ligation dependent probe amplification (clpa)

Family Cites Families (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
ATE144556T1 (de) 1988-06-24 1996-11-15 Amgen Inc Verfahren und mittel zum nachweis von nukleinsäuresequenzen
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
ES2089038T3 (es) 1990-01-26 1996-10-01 Abbott Lab Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa.
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
IL99025A0 (en) 1990-08-15 1992-07-15 Astra Ab Novel process for the detection of pathogens using dna probes
US5683874A (en) 1991-03-27 1997-11-04 Research Corporation Technologies, Inc. Single-stranded circular oligonucleotides capable of forming a triplex with a target sequence
DK51092D0 (da) 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5514545A (en) 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
AU684279B2 (en) 1993-04-12 1997-12-11 Northwestern University Method of forming oligonucleotides
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US6077668A (en) 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5846709A (en) 1993-06-15 1998-12-08 Imclone Systems Incorporated Chemical process for amplifying and detecting nucleic acid sequences
US5808036A (en) 1993-09-01 1998-09-15 Research Corporation Technologies Inc. Stem-loop oligonucleotides containing parallel and antiparallel binding domains
CA2105364A1 (en) 1993-09-01 1995-03-02 Eric T. Kool Stem-loop oligonucleotides containing parallel and antiparallel binding domains
US5824473A (en) 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US5591578A (en) 1993-12-10 1997-01-07 California Institute Of Technology Nucleic acid mediated electron transfer
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
WO1996015271A1 (en) 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
US5674683A (en) 1995-03-21 1997-10-07 Research Corporation Technologies, Inc. Stem-loop and circular oligonucleotides and method of using
US5780613A (en) 1995-08-01 1998-07-14 Northwestern University Covalent lock for self-assembled oligonucleotide constructs
EP2368897B1 (en) 1996-02-09 2016-10-19 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6218108B1 (en) 1997-05-16 2001-04-17 Research Corporation Technologies, Inc. Nucleoside analogs with polycyclic aromatic groups attached, methods of synthesis and uses therefor
EP2369007B1 (en) * 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
ATE308553T1 (de) 1996-06-07 2005-11-15 Amersham Biosciences Sv Corp Nukleinsäurevermittelter elektronentransfer
US6984491B2 (en) 1996-07-29 2006-01-10 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
WO1998020162A2 (en) 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US5922553A (en) 1996-11-21 1999-07-13 Trustees Of The University Of Pennsylvania Method of detecting protein by immuno RNA
US7090804B2 (en) 1998-01-27 2006-08-15 Clinical Mirco Sensors, Inc. Amplification of nucleic acids with electronic detection
EP1060258A1 (en) 1998-03-04 2000-12-20 Onyx Pharmaceuticals, Inc. Baculovirus expression system and method for high throughput expression of genetic material
WO1999055914A1 (en) 1998-04-29 1999-11-04 Trustees Of Boston University Methods and compositions pertaining to pd-loops
US7033753B1 (en) 1999-01-15 2006-04-25 University Of Rochester Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms
US6942771B1 (en) 1999-04-21 2005-09-13 Clinical Micro Sensors, Inc. Microfluidic systems in the electrochemical detection of target analytes
US6297016B1 (en) 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes
US6479650B1 (en) 1999-12-14 2002-11-12 Research Corporation Technologies, Inc. Fluorescent nucleoside analogs and combinatorial fluorophore arrays comprising same
US20050214825A1 (en) * 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
EP1130113A1 (en) 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
EP1313880A2 (en) 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
US6887664B2 (en) 2000-06-06 2005-05-03 Applera Corporation Asynchronous primed PCR
ATE348191T1 (de) 2000-07-03 2007-01-15 Applera Corp Polynukleotid-sequenzassay
CA2439477C (en) * 2001-02-27 2014-04-15 Virco Bvba Circular probe amplification (cpa) using energy-transfer primers
US20050142545A1 (en) 2001-04-11 2005-06-30 Conn Michael M. Methods for identifying small molecules that bind specific rna structural motifs
FI20021325A0 (fi) 2002-07-05 2002-07-05 Valtion Teknillinen Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi
JP2006510348A (ja) 2002-07-18 2006-03-30 ザ・ボード・オブ・トラスティーズ・オブ・ザ・レランド・スタンフォード・ジュニア・ユニバーシティ 蛍光消光脱離基を用いる化学ライゲーションの検出
JP2006500033A (ja) 2002-09-19 2006-01-05 アプレラ コーポレイション 標的を検出するための方法および組成物
WO2004027082A2 (en) 2002-09-19 2004-04-01 Applera Corporation Methods and compositions for detecting targets
US20040259105A1 (en) 2002-10-03 2004-12-23 Jian-Bing Fan Multiplex nucleic acid analysis using archived or fixed samples
EP2460889B1 (en) * 2002-10-11 2013-11-20 Erasmus Universiteit Rotterdam Nucleic acid amplification primers for PCR-based clonality studies of BCL2-IGH rearrangements
US20040235005A1 (en) 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US20040110134A1 (en) 2002-12-05 2004-06-10 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
KR100959101B1 (ko) 2003-02-20 2010-05-25 삼성전자주식회사 Pcr 반응기 및 pcr 반응기의 입구와 출구의 개폐를조절하는 방법
GB0304371D0 (en) 2003-02-26 2003-04-02 Solexa Ltd DNA Sequence analysis
EP1616947B1 (en) * 2003-03-28 2012-05-09 Japan as represented by Director General of National Rehabilitation Center for Persons with Disabilities METHOD OF SYNTHESIZING cDNA
US7198900B2 (en) 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
ATE462801T1 (de) * 2003-11-04 2010-04-15 Applied Biosystems Llc Konkatamere ligationsprodukte: zusammensetzungen, verfahren und kits dafür
EP1702216B1 (en) 2004-01-09 2015-11-04 Life Technologies Corporation Phosphor particle coded beads
US20050208503A1 (en) 2004-03-16 2005-09-22 Handy Yowanto Chemical ligation of nucleic acids
WO2005094532A2 (en) 2004-03-24 2005-10-13 Applera Corporation Encoding and decoding reactions for determining target polynucleotides
WO2006076017A2 (en) 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US7745614B2 (en) * 2004-09-03 2010-06-29 The Board Of Trustees Of The Leland Stanford Junior University Universal linker compositions for the release or transfer of chemical agents from a polynucleotide
US20060223122A1 (en) * 2005-03-08 2006-10-05 Agnes Fogo Classifying and predicting glomerulosclerosis using a proteomics approach
US20060223066A1 (en) * 2005-03-29 2006-10-05 Applera Corporation Methods for normalizing and for identifying small nucleic acids
GB2424946A (en) * 2005-04-05 2006-10-11 Stratec Biomedical Systems Ag A detection system for substance binding using up-converting fluorescent probes
US7799530B2 (en) 2005-09-23 2010-09-21 Celera Corporation Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof
EP2677039B8 (en) * 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Detection of nucleic acid targets using chemically reactive oligonucleotide probes
GB0808413D0 (en) * 2008-05-09 2008-06-18 Univ Nottingham Stabilisation of blood cell conjugates
EP2529026B1 (en) * 2010-01-25 2013-11-13 Rd Biosciences Inc. Self-folding amplification of target nucleic acid
US9506112B2 (en) * 2010-02-05 2016-11-29 Siemens Healthcare Diagnostics Inc. Increasing multiplex level by externalization of passive reference in polymerase chain reactions
ES2623859T3 (es) * 2010-03-04 2017-07-12 Miacom Diagnostics Gmbh FISH múltiple mejorada
US9609769B2 (en) 2010-07-29 2017-03-28 Rockwell Automation Technologies, Inc. System and method for ventilating and isolating electrical equipment
KR102085242B1 (ko) * 2011-05-17 2020-03-05 디엑스테리티 다이아그노스틱스 인코포레이티드 표적 핵산 검출을 위한 방법 및 조성물
WO2012162161A1 (en) * 2011-05-20 2012-11-29 Phthisis Diagnostics Microsporidia detection system and method
WO2014143762A2 (en) 2013-03-15 2014-09-18 Armour Technologies, Inc. Medical device curving apparatus, system, and method of use
FR3054894A1 (fr) 2016-08-03 2018-02-09 Stmicroelectronics (Crolles 2) Sas Dispositif integre photonique a compacite amelioree

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010114599A1 (en) * 2009-04-01 2010-10-07 Dx Terity Diagnostics Inc. Chemical ligation dependent probe amplification (clpa)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J Clin Microbiol. 1996;34(3):501-507

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023182742A1 (ko) * 2022-03-24 2023-09-28 전북대학교산학협력단 역전사를 사용하지 않는 결찰 방법을 이용한 cdna 합성 기반 표적 유전자 검출용 조성물 및 다중 결찰 보조 재조합효소 중합효소 증폭 방법

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