KR102080940B1 - The active ingredient-adsorbed citrus biogel and the preparing method thereof - Google Patents

Abstract

The present invention provides a mask pack comprising a cellulose gel, wherein the mask pack provides a mask pack in which the sperm extract of Pheasant is adsorbed on the surface of the cellulose gel. The mask pack of the present invention has excellent antioxidant activity.

Description

The active ingredient-adsorbed citrus biogel and the preparing method

The present invention relates to a method of adsorbing an active ingredient to a citrus biogel and a citrus biogel to which the active ingredient is adsorbed through such a method, and more particularly, a method of increasing the skin permeability by adsorbing an antioxidant component to the citrus biogel and It would be on the way to produce these citrus biogels.

Cellulose is one of the most abundant biological materials in nature. In particular, cellulose produced by acetic acid bacteria has attracted much attention not only as an additive of food but also as a new value-added industrial new material. Since it has been reported that acetic acid bacteria produce cellulose (Brown. AJ, J. Chem. Soc., Vol. 49, pp. 432-439, 1986), cellulose produced by microorganisms (Bacterial Cellulose) is a new material. Has not been studied.

In particular, mask packs using cellulose produced by acetic acid bacteria have been recognized in the cosmetic industry for high added value. Cellulose mask packs have high skin adhesion and have a great response from consumers with nature-friendly eco-friendly materials.

Meanwhile, in the cosmetics field, a sheet type mask pack is a product that attaches a sheet made in a specific form to a human body without applying cosmetics by hand.

Sheet-type packs have a support that can hold the fluid so that it can act on the skin for a long time as compared to a general cosmetic emulsion, and due to the enhancement of the skin penetration effect of the skin efficacy ingredient by the occlusive effect provided by the support It is a cosmetic product with excellent moisturizing effect and skin beauty effect. Various materials research for differentiation in feeling of use, fit, efficacy, etc. has been attempted.

However, the mask pack of the sheet type does not include all of the active ingredients introduced in the manufacturing process once the mask pack is provided, there is a problem that is discarded while remaining in the cosmetic oil.

Therefore, an object of the present invention is to improve the amount of the active ingredient is delivered to the skin by adsorbing the active ingredient in the mask pack.

The present invention is to provide a method for adsorbing the active ingredient with excellent antioxidant activity to citrus biogel.

The present invention to solve the above problems, to provide a mask pack characterized in that the active ingredient is adsorbed on the cellulose gel.

The present invention provides a mask pack containing a cellulose gel, wherein the pheasant extract of the pheasant is adsorbed on the surface of the cellulose gel.

In a preferred embodiment, the present invention is adsorbed on the surface of the cellulose gel adsorbed by the pheasant extract, preferably polyphenols, carotenoids and other components, more preferably hesperidin, quercetin, resveratrol, isoflavones, catechins and other polyphenols, carotenoids and the like To provide a mask pack.

The cellulose gel may be a citrus cellulose gel. The cellulose gel is present in coconut-derived cellulose gel, etc., in particular, the use of citrus-derived cellulose gel was found to be excellent in the adsorption of the active ingredient to complete the present invention.

As used herein, the term "adsorption" may be understood to mean a phenomenon in which a target component is collected on the surface of a cellulose gel. Due to the influence of pores of the cellulose gel, it can be seen that the target components are gathered on the surface of the portion where the pores are formed, the target components can be included in the interior of the cellulose gel, even in this case the scope of the present invention It can be understood that it is not excluded from.

'Cellulose gel' as used herein refers to a cellulose gel prepared by using citrus juice or by-products remaining after citrus juice, and, for example, by using as a medium the residual residue remaining after citrus juice or citrus juice. Gluconacetobacter sp. Gel_SEA623-2 (KACC 91526P) inoculated in the medium can be understood to mean a cellulose gel produced.

The inventors of the present invention confirmed that the pheasant extract of Pheasant has excellent antioxidant activity, but it is not easy to deliver using a mask pack, and thus, the present invention has been completed.

The inventors of the present invention have confirmed that when a certain treatment is applied to the cellulose gel, the sperm extract of the pheasant is adsorbed on the surface of the cellulose gel and can be easily delivered to the skin, thereby completing the present invention.

In particular, citrus-derived cellulose gel has been found to be particularly excellent for the purpose of the present invention because of the excellent elasticity of the gel and the property of not being easily deformed.

Preferably the sperm extract of the pheasant is a flavonoid. It contains a large amount of antioxidant components, such as capotenoid, and particularly contains a large amount of quercetin, carotene, hesperidin and the like, and it was confirmed that the adsorption of hesperidin on the citrus-derived cellulose gel surface through the treatment method of the present invention.

Through one embodiment of the present invention it is possible to provide a mask pack in which hesperidin is adsorbed on the surface of the mask pack and delivered to the skin.

According to one embodiment of the present invention, it is possible to provide a method of manufacturing a mask pack comprising a sperm extract and cellulose gel of pheasant.

Preferably, the cellulose gel may be subjected to a step of immersing S1) a cellulose gel in an acidic solution, and (S2) immersing the cellulose gel immersed in the step (S1) in a cosmetic composition containing the amaranth extract of Pheasant. .

Further (S3) the cellulose gel of step (S2) can be heated to a temperature of 90 to 130 ℃, preferably at a temperature of 95 to 128 ℃, more preferably to a temperature of 98 to 125 ℃ high temperature treatment have. In the above temperature range, the amount of sperm extract, preferably hesperidin of pheasant adsorbed on the mask pack may be improved.

Preferably the acidic solution of step (S1) may be pH 2.5 to 6, preferably pH 3.5 to 5.5, more preferably may be about pH 4-5.

When the mask pack is immersed in the above pH conditions, the amount of sperm extract, preferably hesperidin of the pheasant adsorbed to the mask pack can be improved.

Due to the nature of the mask pack, the contamination may be caused by an external contaminant. However, a conventional mask pack is emulsified using at least one selected from, for example, phenoxy ethanol, methyl paraben, ethyl paraben, propyl paraben, butyl paraben and 1,2-hexanediol. Compared to the treatment, the mask pack of the present invention is substantially free of such ingredients due to the excellent antiseptic and antioxidant effects of the Pheasant's amaranth extract

The cosmetics applicable to the biocellulose sheet according to the present invention are not particularly limited and may include conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and / or flavors. Biocellulose containing the amaranth extract component of the pheasant of the present invention can be processed in the form of a mask using a molding machine to produce a facial mask pack.

The mask pack of the present invention may be further subjected to molding, folding, inserting, filling, and packaging process steps, which are essential processes for manufacturing a cosmetic mask pack.

The mask pack of the present invention has excellent antioxidant activity.

The mask pack of the present invention can improve the skin delivery rate of the antioxidant active ingredient included in the cosmetic composition.

Figure 1 shows the appearance of the pheasant extract adsorbed on the citrus cellulose gel.
Control refers to a solution that does not contain an extract.
2 is a photograph comparing the coconut cellulose gel and citrus cellulose gel.

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, embodiments according to the present invention can be modified in many different forms, the scope of the invention should not be construed as limited to the following examples. Embodiments of the present invention are provided to more fully explain the present invention to those skilled in the art.

Citrus Cellulose gel  Ready]

SEA623-2 strain of Gluconacetobacter sp., Deposited under accession number KACC 91526P, was inoculated at 1 × 10 ⁵ cells / ml in a sugar concentration of 10 brix and an acidity pH 3.0 (10% (v / v)). Incubated at 20 days in aerobic conditions. As a result, the initial gel formation rate of the strain was very excellent, and the gel membrane was formed from the second day of the culture, and the experiment was performed using a cellulose gel having a thickness of about 8 mm.

Example  Citrus Biogel  pH composition check

First, various pH environments were created. pH was set to 3-10. To make accurate pH conditions

pH 3, 4, 5: sodium citrate buffer,

pH 6,7,8: sodium phosphate buffer,

pH 9, 10: Glycine-NaOH buffer was used.

Ethanol containing 25% glycerol was prepared so that the concentration of the buffer solution to 0.1M. Carotene, hesperidin, gallic acid, vitamin C was used for the experiment.

Four compounds were treated for useful ingredient adsorption in citrus biogels. Compounds treated are carotene, hesperidin, gallic acid, vitamin C. These compounds were adsorbed onto citrus biogel at a concentration of 5 mg / mL to confirm the degree of subsequent adsorption.

Example  2. Check the adsorption power according to pH

After putting the citrus biogel cut to 1cm in all directions to the pH of the composition conditions, and left at 25 ℃ overnight, the antioxidant activity was measured to confirm the adsorption capacity. In order to confirm the adsorption capacity, the compounds used in the study were compounds having excellent antioxidant activity.

DPPH radical scavenging activity was confirmed by the following method.

After the adsorption test was washed three times with distilled water and put the citrus biogel adsorbed in a useful component in a 24-well plate, 500 μL of DPPH reaction solution was added for 10 minutes and the absorbance was measured at 517 nm. This was repeated to confirm the degree of activity maintained. It was confirmed that the activity was maintained by repeating six times.

As a result, the adsorption power was the best at pH 4 and 5, it was confirmed that the adsorption power is maintained even after repeated six times or more to show the antioxidant activity.

 pH beta carotene  hesperidin  gallic acid  Vitamin C
3   39.75   40.20   65.42   68.05
4   54.45   42.48   83.92   85.60
5   52.32   41.45   80.89   83.65
6   47.30   37.93   77.08   79.86
8   32.76   28.23   4.92   0.68
10   35.92   31.57   73.94  79.26

Example  3. Excellent adsorption pH pH4 and  Adsorption force at 25, 37, 50, and 100 ° C (1 atm), and 121 ° C (1.5 atm) to confirm the adsorption power according to the temperature at 5

Small molecular weight gallic acid and vitaminC showed high adsorption capacity regardless of temperature, and vitaminC seems to be less stable when heat is applied. However, carotene and hesperidin, which have high molecular weights, were found to increase their adsorption capacity when heated. It was confirmed that the proper pH was the best adsorption power at 4, 5.

In order to confirm the degree of adsorption by temperature, the tests were carried out at pH conditions 4 and 5, which were excellent in adsorption power. In addition, when the pressure is applied, it was confirmed that the adsorption power of the compound having a large molecular weight increases.

Table 2 shows the results of confirming the adsorption degree of carotene and hesperidin according to the temperature.

  pH4   pH5
Temperature (℃)
beta carotene
hesperidin
beta carotene
hesperidin
25   54.45   42.48   39.75   41.45
37   56.53   56.72   50.98   53.19
50   61.13   58.93   58.47   58.23
100   69.73   68.4   60.11   66.6 121   75.01   77.8   69.24   76.2 130   40   38   37   35

Table 3 shows the activity when 121 ° C. heat was applied to gallic acid and vitamin C.

pH   gallic acid   ascorbic acid
3
93.75
41.29
4
93.58
47.48
5
92.07
46.20
6
93.15
41.47
8
75.12
45.12
10
93.07
40.30

Example  4. Check the release degree according to the temperature of the adsorbed compounds. At body temperature (37 degrees Celsius)  Confirm

In addition, when the activity was measured at room temperature and skin temperature to confirm the release degree of the compound, it was confirmed that the release was more than 20% better than room temperature. It is thought that it will be easy to release useful ingredients slowly and continuously when adsorbing useful ingredients to biogel with excellent adhesion to skin.

Table 4 shows the activity according to the release temperature. (Measure activity after 20 minutes using DPPH)

  25 degrees (℃)   36.5 degrees (℃)
pH
beta carotene
hesperidin
beta carotene
hesperidin
4
54.45
42.48
67.13
53.46
5
39.75
41.45
59.65
61.10

Example  5. Biogel  Further experimentation as to whether or not stable adsorption was achieved within

Stability experiments were conducted to confirm that there was no temporary adhesion to the biogel surface. After standing for 70 days at 4 ℃ activity was confirmed.

Table 5 shows the results of adsorption test according to pH after leaving the biogel adsorbed the active ingredient at 4 ℃ for 70 days.

  pH  beta carotene  hesperidin  gallic acid  ascorbic acid
3
4,12
-
86.23
62.312
4
4.755
-
90.399
77.236
5
6.245
-
87.049
89.567
6
6.521
-
84.122
33.738
8
-
-
65.785
24.392
10
-
-
26.518
-

Table 6 below shows the activity measured after standing at 4 ° C. for 70 days at 121 ° C. and treated with 1.5 atm.

pH
beta carotene
hesperidin
gallic acid
ascorbic acid
3
40.322
2.331
87.231
89.635
4
73.681
13.804
90.134
90.139
5
71.371
11.43
88.183
87.674
6
60.664
7.114
85.453
90.961
8
59.641
1.962
68.059
72.290
10
-
5.058
37.216
52.982

When treated under the conditions of the present invention, it was confirmed that the unstable hesperidin has stability over time even at low temperatures.

Example  6. Pheasant's Purslane  Adsorption Experiment of Extract

When the extract of pheasant with excellent antioxidant activity was adsorbed onto the biogel, the anti-oxidant activity was also excellent at pH 4, and the antioxidant activity was excellent. .

As can be seen from the above results, the general beta carotene and hesperidin was not easily attached to the cellulose gel when compared with vitamin C and gallic acid, but adjusted the pH of the aqueous solution containing beta carotene and hesperidin to 4-5, 121 ℃ When the optimum conditions were established by heating the sample at 1.5 atm, the adsorption rate was high.

In addition, as a method of measuring the total polyphenol content of the biogel adsorbed by the pheasant extract of the pheasant, it was possible to identify the biogel that changes color due to the adsorption of the pheasant.

Example  7. Citrus With cellulose gel Coconnet  cellulose Gel  Comparative experiment

The mask pack manufactured using the coconut gel was rough and had a lot of foreign substances, making it difficult to use as a mask pack. In addition, it was difficult to adsorb the active ingredient as in the present invention.

However, the citrus cellulose gel of the present invention was suitable for use as a mask pack because the gel particles are thin and have no foreign matter.

Claims (10)

delete delete delete delete In the method for producing a mask pack containing beta carotene, hesperidin, or a mixture thereof and citrus cellulose gel, wherein the beta carotene, hesperidin, or a mixture thereof is adsorbed on the citrus cellulose gel,
(S1) immersing the citrus cellulose gel in an acidic solution,
(S2) immersing the cellulose gel immersed in the step (S1) in the cosmetic composition containing beta carotene, hesperidin, or a mixture thereof; And
(S3) Method for producing a mask pack adsorbed beta carotene, hesperidin, or a mixture thereof comprising heating the citrus cellulose gel of step (S2) to a temperature of 98 to 125 ℃. delete The method of claim 5, wherein the acidic solution of the step (S1) is a mask pack manufacturing method, characterized in that the pH 2.5 to 6. The method of claim 7, wherein the acidic solution of the step (S1) is a mask pack manufacturing method, characterized in that the pH 3.5 to 5.5. delete delete

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