KR102070854B1 - Composition for preventing, improving or treating muscular damage comprising Vigna angularis extract - Google Patents

Abstract

The present invention confirms the effect of red bean extract or adzuki bean-derived peptides on damaged muscle cells, and the red bean extract or red bean-derived peptides promote the activation of muscle satellite cells in the damaged muscles during administration. It may be usefully used as a composition, a food composition for preventing or improving, a cosmetic composition and a pharmaceutical composition for promoting regeneration of an injured muscle, a food composition or a cosmetic composition.

Description

Composition for preventing, improving or treating muscular damage comprising Vigna angularis extract}

The present invention relates to a composition for preventing, improving or treating muscle damage, comprising a red bean extract, and more particularly, a composition for preventing or improving muscle damage pharmaceutical composition comprising the red bean extract or a red bean-derived peptide as an active ingredient. It relates to a cosmetic composition or a pharmaceutical composition for regenerating injured muscle, a food composition, and a cosmetic composition.

Muscle is a tissue produced by the development of mesenchymal stem cells and consists of a bundle of myofibers composed of fusion of myoblasts. Muscles make up 40-50% of the body's weight, supporting and protecting bones and organs, as well as motility of other tissues, such as the beating of the heart (J. Nutr. Sci. Vitaminol., 61: 188-194, 2015). In addition to the protection of organs, muscles are also closely linked to the development of metabolic diseases such as type 2 diabetes, obesity, and cardiovascular disease because muscles have a significant effect on metabolism as well as physical activities including exercise. Due to this importance, muscle regeneration and recovery following muscle damage and injury are essential for the maintenance of normal body function (Am. J. Cardiol., 110: 58B-68B, 2012; Br. J. Pharmacol., 170: 1153-1166, 2013).

When muscles are injured or damaged, muscle satellite cells, precursors to muscle cells, play an important role in muscle regeneration. Normally, muscle satellite cells located at the edges of muscle fibers remain quiescent, but secrete a variety of transcription factors to regenerate when muscles are physically or chemically damaged from the outside. enter the regeneration phase.

Satellite cells self-renewal by expressed transcription factors and form a stem cell pool for muscle regeneration. At this time, the number of satellite cells required for muscle regeneration is kept constant. In order to increase the expression of Paired Box 7 (Pax7). Increased expression of Pax7 is methylated by coactivator-associated arginine methyltransferase 1 (CARM1) protein to form the Pax7-CARM1 complex to promote the expression of myogenic factor 5 (Myf5). Due to the increased expression of Myf5 factor, muscle satellite cells that were at rest are activated to migrate to wounds and damaged muscle areas. Muscle fibers are then formed to produce new muscle (Stem Cells Transl. Med., 5: 282-290, 2016).

One of the representative legumes, Vigna angularis , is a perennial herb that belongs to the Vigna spp. Of the Fabaceae family and is the second most important legume crop. Red beans are rich in vitamin B1, so if you mix rice with rice, it will give your rice a lack of vitamins. Therefore, the effects of preventing, improving and treating each disease are known to be effective in recovering fatigue. Saponin contained in red beans has the effect of helping with bowel movement along with fiber, and it is also used to relieve intestines by releasing poison and promoting bowel movements, and to improve kidney disease or hangover (Korean. J. Food. Sci. ): 693-698, 2010). In addition, the red beans are antioxidant (J. Food Lipids, 11 (4): 278-286, 2004), antidiabetic (Biosci. Biotechnol. Biochem., 68 (12): 24212426, 2004), antibacterial (Phytother. Res., 20 (2): 162164, 2006), whitening (Int. J. Mol. Sci., 12 (10): 7048-7058, 2011), but has been reported to prevent, treat or No improvement has been reported on efficacy.

Therefore, the present inventors conducted research using various natural materials to develop a composition capable of preventing, improving or treating injured muscles. As a result, the extract extracted from red beans promotes the activation of muscle satellite cells, resulting in muscle damage. The present invention was completed by confirming that there is an effect of preventing, improving or treating and promoting regeneration.

Accordingly, it is an object of the present invention to provide a pharmaceutical composition for preventing or treating muscle damage, comprising red bean extract or red bean-derived peptide as an active ingredient.

In addition, another object of the present invention is to provide a food composition for preventing or improving muscle damage, including red bean extract or red bean-derived peptide as an active ingredient.

In addition, another object of the present invention to provide a cosmetic composition for preventing or improving muscle damage comprising a red bean extract or a red bean-derived peptide as an active ingredient.

Another object of the present invention is to provide a pharmaceutical composition for promoting regeneration of injured muscle comprising red bean extract or red bean derived peptide as an active ingredient.

It is another object of the present invention to provide a food composition for promoting regeneration of injured muscle comprising red bean extract or red bean-derived peptide as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for promoting regeneration of injured muscle comprising red bean extract or red bean derived peptide as an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or treating muscle damage, comprising red beans extract or red beans derived peptide as an active ingredient.

In order to achieve the another object of the present invention, it provides a food composition for preventing or improving muscle damage comprising a red bean extract or a red bean-derived peptide as an active ingredient.

In order to achieve the another object of the present invention, it provides a cosmetic composition for preventing or improving muscle damage comprising a red bean extract or a red bean-derived peptide as an active ingredient.

In order to achieve another object of the present invention, there is provided a pharmaceutical composition for promoting regeneration of injured muscle comprising red bean extract or red bean-derived peptide as an active ingredient.

In order to achieve the another object of the present invention, it provides a food composition for promoting the regeneration of injured muscle comprising red beans extract or red beans derived peptide as an active ingredient.

In order to achieve the another object of the present invention, it provides a cosmetic composition for promoting the regeneration of injured muscle comprising red beans extract or red beans derived peptide as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for preventing or treating muscle damage, comprising red bean extract or red bean-derived peptide as an active ingredient.

The pharmaceutical composition according to the present invention may be a composition comprising an adzuki bean extract or an adzuki bean-derived peptide as an active ingredient, or may be a composition consisting of an adzuki bean extract or an adzuki bean-derived peptide as an active ingredient, or an adzuki bean extract or an adzuki bean as an active ingredient It may also be a composition in which the derived peptide consists essentially.

As used herein, the term 'comprising' is used in the same sense as 'including' or 'characterized by,' specifically referring to the composition or method according to the present invention. It does not exclude additional components or steps of the method that are not. In addition, the term 'consisting of' means to exclude additional elements, steps or components, etc., unless otherwise noted. The term 'essentially consisting of' means that in the scope of the composition or method, it may include the substance or step described, as well as the substance or step which does not substantially affect its basic properties. .

As used herein, “muscle” is a generic term for tendons, muscles, and tendons, and is not limited to skeletal muuscles, but includes cardiac muscles and smooth muscles.

As used herein, “muscle regeneration” refers to the rapid recovery of muscles when they are physically or chemically damaged, and refers generically to the process and duration of time until the damaged muscles can function normally.

As used herein, 'red bean ( Vigna angularis )' is a red bean ( V.angularis WFWight), black bean (V. angularis var. Angularis) or other belonging to the genus Vigna spp. The seeds of plants are dried and may be used alone or in combination.

 As used herein, the term “red bean extract” refers to an extract obtained by extracting red beans. The preparation method of the red bean extract may be obtained by extracting the red bean with one or more solvents selected from the group consisting of water, an organic solvent of C1 to C6, a subcritical fluid and a supercritical fluid. More specifically, the solvent is water, alcohol having 1 to 6 carbon atoms (alcohol), acetone (acetone), ether (ether), benzene (benzene), chloroform (chloroform), ethyl acetate (ethyl acetate), methylene chloride (methylene chloride), hexane, cyclohexane, and petroleum ether, but may be one or more selected from the group consisting of, but is not limited thereto.

In addition, the red bean extract of the present invention can be obtained by extracting and refining the red bean using purified water, ethanol and subcritical water or supercritical carbon dioxide suitable for food processing, or by separating and refining from the oil obtained by directly compressing the red bean. . Red beans extract according to the present invention may be obtained by ultra-high pressure treatment and extraction for 1 to 24 hours at a temperature of 40 to 90 ℃ red beans at a pressure of 100 to 1000 MPa, but is not limited thereto.

In addition, the red bean extract is selected from the group consisting of Alcalase, Flavorzyme, Neutrase, Protamex and Protease-NP It can be prepared by treating any one or more proteolytic enzymes, it is possible to activate the enzyme at a pH condition of 2 to 8 and temperature conditions of 30 to 60 ℃, but is not limited thereto.

The extract of the present invention may be used as a liquid by filtration and / or concentration, and may be used by solidifying through a conventional drying process such as spray drying or lyophilization. In the drying process may be mixed by spray drying or dextrin prior to freeze drying.

The composition according to one embodiment of the present invention may promote the activation of muscle satellite cells. Specifically, when muscles are damaged, muscle satellite cells are activated through Pax7 and moved to the damaged areas of the muscles to induce recovery of the damaged areas. As a result of evaluating the muscle regeneration activity of the red beans in order to confirm the activation of the muscle satellite cells, the peptide isolated from the red beans not only increased the mRNA of Pax7, but also the red bean extract quickly recovered the muscles, thus making the muscle satellite as normal. The nucleus of the cell was placed at the edge of the muscle fiber. Therefore, it was confirmed that the red bean extract quickly recovered muscles from damaged muscles. This means that the red bean extract according to the present invention has an advantage in strengthening muscle regeneration or recovery.

As used herein, the term “red bean-derived peptide” means one obtained by treating proteolytic enzymes on red bean protein isolated from red beans.

In the present specification, the 'hydrolase' is composed of Alcalase, Flavorzyme, Flavorzyme, Neutrase, Protamex, and Protease-NP. It may be any one or more proteolytic enzyme selected from the group, preferably protamex.

In the present specification, 'red bean-derived peptide' may be specifically obtained by the method consisting of the following steps:

i) milling the dried red beans and extracting with hexane as a solvent;

ii) removing the hexane extract extracted in step i) and adding water to the foil and leaving it at pH 7.0 to 10.0;

iii) centrifuging the solution left in step ii) to obtain a supernatant;

iv) leaving the supernatant obtained in step iii) again at pH 2.0 to 6.0;

v) centrifuging the solution left in step iv) to obtain precipitated precipitate as red beans derived protein;

vi) adding a hydrolase to the red bean-derived protein obtained in step v) and performing an enzymatic reaction, followed by filtration to remove the precipitate; And

vii) lyophilizing the filtrate from which the precipitate is removed in step vi) to obtain a peptide.

In the present specification, "muscle damage" may be exercise-induced muscle damage. Muscle damage can generally be defined as loss of muscle function due to physical destruction of muscle structure after activity that causes certain injuries, which can cause trauma, excessive temperature, injury from mytoxins, ischemia, muscle Related diseases, inflammation, exercise, etc. can be various. Among these, exercise-induced muscle injury can be classified as primary muscle damage causing morphological or structural changes in the muscle, and secondary muscle damage causing inflammatory reactions and loss of calcium homeostasis. Can be. Primary muscle injuries include sarcomere and structural destruction of cytoskeletal elements, weakening the development of muscle immediately after muscle injury. Secondary muscle damage occurs in the process of repairing primary damage, which causes delayed onset muscle soreness (DOMS) by the inflammatory response and not only releases muscle protein into the plasma, but also results in persistent muscle loss, resulting in temporary muscle function. It will cause a loss.

In addition, the muscle injury may include muscle strain, muscle rupture, muscle tearing, contusion, distortion, roator cuff syndrome, and myositis. It may be selected from the group consisting of, but is not limited thereto.

The pharmaceutical composition for preventing or treating muscle injury of the present invention may further include a pharmaceutically acceptable carrier.

Pharmaceutically acceptable carriers may further include, for example, carriers for oral administration or carriers for parenteral administration. Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid and the like. Carriers for parenteral administration may also include water, suitable oils, saline, aqueous glucose, glycols, and the like. In addition, stabilizers and preservatives may be further included. Suitable stabilizers include pharmaceutically acceptable carriers such as, for example, sodium bisulfite, sodium sulfite or ascorbic acid, and may further include, for example, carriers for oral administration or carriers for parenteral administration. Carriers for oral administration may include lactose, starch, cellulose derivatives, magnesium stearate, stearic acid and the like. Carriers for parenteral administration may also include water, suitable oils, saline, aqueous glucose, glycols, and the like. In addition, stabilizers and preservatives may be further included. Suitable stabilizers include antioxidants such as sodium hydrogen sulfite, sodium sulfite or ascorbic acid. Suitable preservatives include benzalkonium chloride, methyl- or propyl-parabens and chlorobutanol. Other pharmaceutically acceptable carriers may be referred to those described in the following literature (Remington's Pharmaceutical Sciences, 19th ed., Mack Publishing Company, Easton, PA, 1995).

The pharmaceutical compositions of the present invention can be administered to any mammal, including humans. For example, it can be administered orally or parenterally, parenteral administration methods include, but are not limited to, intravenous, intramuscular, intraarterial, intramedullary, intradural, intracardiac, transdermal, subcutaneous, intraperitoneal , Intranasal, intestinal, topical, sublingual or rectal administration.

The pharmaceutical composition of the present invention may be formulated into a preparation for oral or parenteral administration according to the route of administration as described above. When formulated, one or more buffers (e.g. saline or PBS), antioxidants, bacteriostatic agents, chelating agents (e.g. EDTA or glutathione), fillers, extenders, binders, adjuvants (e.g. aluminum hydroxide) Side), suspending agents, thickening humectants, disintegrants or surfactants, diluents or excipients.

Solid form preparations for oral administration include tablets, pills, powders, granules, solutions, gels, syrups, slurries, suspensions, or capsules, and the like may include at least one excipient in the pharmaceutical composition of the present invention. For example, starch (including corn starch, wheat starch, rice starch, potato starch, etc.), calcium carbonate, sucrose, lactose, dextrose, sorbitol, mannitol, xylitol, erythritol maltitol, It can be prepared by mixing cellulose, methyl cellulose, sodium carboxymethyl cellulose and hydroxypropylmethyl-cellulose or gelatin. For example, tablets or dragees can be obtained by combining the active ingredients with solid excipients and then grinding them, adding suitable auxiliaries and processing them into a granule mixture.

In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Oral liquid preparations include suspensions, solutions, emulsions, or syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, or preservatives, in addition to commonly used simple diluents, water or liquid paraffin. .

In addition, in some cases, crosslinked polyvinylpyrrolidone, agar, alginic acid or sodium alginate may be added as a disintegrant, and may further include anti-coagulants, lubricants, wetting agents, fragrances, emulsifiers, and preservatives. .

When administered parenterally, the pharmaceutical compositions of the present invention may be formulated according to methods known in the art in the form of injections, transdermal and nasal inhalants with suitable parenteral carriers. Such injections must be sterile and protected from contamination of microorganisms such as bacteria and fungi. Examples of suitable carriers for injections include, but are not limited to, solvents or dispersion media comprising water, ethanol, polyols (e.g., glycerol, propylene glycol and liquid polyethylene glycols, etc.), mixtures thereof and / or vegetable oils Can be. More preferably, suitable carriers include Hanks solution, Ringer's solution, phosphate buffered saline (PBS) containing triethanol amine or sterile water for injection, 10% ethanol, 40% propylene glycol and 5% dextrose Etc. can be used. In order to protect the injection from microbial contamination, it may further include various antibacterial and antifungal agents such as parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In addition, the injection may in most cases further comprise an isotonic agent, such as sugar or sodium chloride.

In the case of transdermal administrations, ointments, creams, lotions, gels, external preparations, pasta preparations, linen preparations, air rolls and the like are included. As used herein, "transdermal administration" means that the pharmaceutical composition is applied to the skin topically so that an effective amount of the active ingredient contained in the pharmaceutical composition is delivered into the skin.

For inhaled dosages, the red bean extract or red bean derived peptide of the invention is pressurized using a suitable propellant such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. It can be conveniently delivered in aerosol spray form from a pack or nebulizer. In the case of a pressurized aerosol, the dosage unit can be determined by providing a valve to deliver a metered amount. For example, gelatin capsules and cartridges used for inhalers or blowers can be formulated to contain a mixture of the compound and a suitable powder base such as lactose or starch. Formulations for parenteral administration are described in Remington's Pharmaceutical Science, 15th Edition, 1975. Mack Publishing Company, Easton, Pennsylvania 18042, Chapter 87: Blaug, Seymour, a prescription generally known in all pharmaceutical chemistries.

The pharmaceutical composition for preventing and treating muscle damage of the present invention may provide a desirable muscle damage preventing and treating effect when an effective amount of red bean extract or red bean-derived peptide is included. As used herein, the term 'effective amount' refers to an amount that exhibits a higher response than a negative control, and preferably an amount sufficient to recover and regenerate damaged muscle. The pharmaceutical composition of the present invention may contain 0.001 to 99.99% of red beans extract or red beans derived peptides, and the remaining amount may be occupied by a pharmaceutically acceptable carrier. The effective amount of the red bean extract or red bean derived peptide contained in the pharmaceutical composition of the present invention will vary depending on the form of the composition and the like.

More preferably, the dose of the red bean extract or the red bean-derived peptide in the composition according to the present invention may be included at a concentration of 0.001% to 50%, but is not limited thereto.

In this case, when the red bean extract or the red bean-derived peptide is less than the concentration range, there is a problem that it is difficult to exert the effect of preventing or treating muscle damage, and when the red bean extract or the red bean-derived peptide exceeds the concentration range, the toxicity including cytotoxicity There may be concerns.

Preferred dosages of the pharmaceutical composition for preventing or treating muscle injury according to the present invention vary depending on the condition, body weight, degree of disease, drug form, route of administration and duration of the patient, and may be appropriately selected by those skilled in the art. However, for the desired effect, it may be administered at 0.0001 to 2,000 mg / kg, preferably at 0.001 to 2,000 mg / kg. Administration may be administered once a day or may be divided several times. However, the scope of the present invention is not limited by the above dosage. The pharmaceutical composition according to the present invention is not particularly limited to its formulation, route of administration and method of administration as long as the effect of the present invention is shown.

The pharmaceutical composition for preventing or treating muscle damage according to the present invention can be administered to mammals such as mice, mice, livestock, humans, etc. by various routes. All modes of administration can be administered, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

The pharmaceutical composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy or biological response modifiers.

As used herein, "treatment" refers to a clinical procedure to alter the natural process of the individual or cell being treated, and may also be performed for the prevention of clinical pathology. Desirable effects of treatment include: inhibiting the occurrence or recurrence of the disease, alleviating symptoms, reducing any direct or indirect pathological consequences of the disease, decreasing the rate of disease progression, improving the disease state, improving, alleviating or improving the prognosis, and the like. Include. The term 'prevention' also means any action that inhibits the onset or delays the progression of a disease.

The pharmaceutical composition of the present invention may also be provided in a formulation of an external preparation comprising red beans extract or red beans derived peptide as an active ingredient.

When the pharmaceutical composition of the present invention is used as an external preparation for skin, it is additionally used for fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, interfaces. Skin such as active agents, water, ionic emulsifiers, nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic active agents, lipophilic active agents or lipid vesicles It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients commonly used in external preparations. The ingredients may also be introduced in amounts generally used in the field of dermatology.

When the pharmaceutical composition of the present invention is provided as an external preparation for skin, it may be a formulation such as, but not limited to, ointment, patch, gel, cream or spray.

In another aspect, the present invention provides a food composition for preventing or improving muscle damage comprising a red bean extract or a red bean-derived peptide as an active ingredient.

The red bean extract for preparing the food composition is as described above. The food composition of the present invention includes all forms such as functional food, nutritional supplement, health food, food additives and feed, and includes animals including humans or livestock. It is for eating. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

Food compositions of this type can be prepared in various forms according to conventional methods known in the art. General foods include, but are not limited to, beverages (including alcoholic beverages), fruits and processed foods (e.g. canned fruit, canned foods, jams, marmalade, etc.), fish, meat and processed foods (e.g. hams, sausages) Cornbeans, etc.), breads and noodles (e.g. udon, soba noodles, ramen, spagate, macaroni, etc.), fruit juices, various drinks, cookies, malts, dairy products (e.g. butter, cheese), edible vegetable oils, margarine It may be prepared by adding the red bean extract to vegetable protein, retort food, frozen food, various seasonings (eg, miso, soy sauce, sauce, etc.). In addition, as a nutritional supplement is not limited to this can be prepared by adding the red bean extract to capsules, tablets, pills. In addition, as a health functional food, for example, the red bean extract or the red bean-derived peptide itself is prepared in the form of tea, juice and drink to be liquefied, granulated, encapsulated and powdered for drinking (health drink). It can be consumed by becoming angry. In addition, in order to use the red bean extract or the red bean-derived peptide in the form of a food additive, it may be prepared and used in powder or concentrate form. In addition, the red bean extract or the red bean-derived peptide may be mixed with a known active ingredient known to have a restorative effect on the injured muscle, and thus prepared in the form of a composition.

In general, the red bean extract may be added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less based on 100 parts by weight of the raw material in the manufacture of food or beverage. However, in the case of long-term intake for health and hygiene or for health control, the amount may be below the above range, and the present invention has no problem in terms of safety in terms of using fractions from natural products. The above amount can also be used.

The beverage in the food according to the present invention may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general drinks. The natural carbohydrates described above may be glucose, monosaccharides such as fructose, maltose, disaccharides such as sucrose and polysaccharides such as dextrin, cyclodextrin, sugar alcohols such as xylitol, sorbitol, erythritol. As the sweetener, natural sweeteners such as tautin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like can be used. The ratio of the natural carbohydrate may be about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 mL of the beverage according to the present invention.

In addition to the above, the food for preventing or improving muscle damage according to the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acids, protective colloid thickeners, and pH adjusting agents. , Stabilizers, preservatives, glycerin, alcohols or carbonating agents and the like. In addition, the health food of the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage or vegetable beverage. These components can be used independently or in combination. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

In another aspect, the present invention provides a cosmetic composition for preventing or improving muscle damage comprising a red bean extract or a red bean-derived peptide as an active ingredient.

The red bean extract or red bean derived peptide for preparing the cosmetic composition is as described above. The cosmetic composition of the present invention contains an adzuki bean extract or an adzuki bean-derived peptide as an active ingredient, and with a dermatologically acceptable excipient, a basic cosmetic composition (washing agent such as cosmetic water, cream, essence, cleansing foam and cleansing water, pack, body oil) , Color cosmetic compositions (foundation, lipstick, mascara, makeup base), hair product compositions (shampoos, rinses, hair conditioners, hair gels), soaps and the like.

The excipients include, but are not limited to, emollients, skin penetration enhancers, colorants, fragrances, emulsifiers, thickeners and solvents. In addition, fragrances, pigments, fungicides, antioxidants, preservatives and humectants may be further included, and may include thickeners, inorganic salts, synthetic polymer materials and the like for the purpose of improving the properties. For example, in the case of preparing the face wash and the soap with the cosmetic composition of the present invention, it can be easily prepared by adding the red bean extract to a conventional face wash and soap base. When the cream is prepared, it can be prepared by adding a red bean extract or a salt thereof to a cream base of a general oil-in-water type (O / W). To this, synthetic or natural materials such as proteins, minerals, vitamins, etc., for the purpose of improving physical properties, such as flavors, chelating agents, pigments, antioxidants, and preservatives, may be added. The content of the red bean extract contained in the cosmetic composition of the present invention is not limited thereto, but is preferably 0.001 to 10% by weight, and more preferably 0.01 to 5% by weight based on the total weight of the composition. If the content is less than 0.001% by weight, the desired damage muscle treatment / improvement effect cannot be expected, and if it is more than 10% by weight, there may be difficulty in preparing safety or formulation.

The present invention also provides a pharmaceutical composition for promoting regeneration of injured muscle comprising red bean extract or red bean derived peptide as an active ingredient.

The method for preparing the red bean extract or the red bean-derived peptide and the red bean extract or the red bean-derived peptide are as described above, and may be included in the pharmaceutical composition according to the present invention. The method of administration such as the amount is as described above.

The pharmaceutical composition according to the present invention may be a composition comprising an adzuki bean extract or an adzuki bean-derived peptide as an active ingredient, or may be a composition consisting of an adzuki bean extract or an adzuki bean-derived peptide as an active ingredient, or an adzuki bean extract as an active ingredient The composition may consist essentially of.

In addition, the present invention provides a food composition for promoting regeneration of damaged muscle comprising red bean extract or red bean derived peptide as an active ingredient.

The method of preparing the red bean extract or the red bean-derived peptide and the red bean extract or the red bean-derived peptide are the same as described above, and the same as the above-described examples and contents of the food composition.

In addition, the present invention provides a cosmetic composition for promoting regeneration of damaged muscle comprising red bean extract or red bean derived peptide as an active ingredient.

The method for preparing the red bean extract or the red bean-derived peptide and the red bean extract or the red bean-derived peptide are the same as described above, and the same as the above-described examples and contents of the cosmetic composition.

Accordingly, the present invention has been confirmed that the effect of red beans extract or red beans derived peptides on the damaged muscle cells, the red beans extract or red beans derived peptides promote the recovery of muscles by promoting the activation of muscle satellite cells in the damaged muscles during administration It can be usefully used as a pharmaceutical composition for preventing or treating muscle damage, a food composition for preventing or improving, a cosmetic composition and a pharmaceutical composition for promoting regeneration of damaged muscle, a food composition or a cosmetic composition.

1 is a result confirming the effect of red beans peptides on Pax7 mRNA expression in muscle satellite cells isolated from the hind legs of the mouse.
Figure 2 is a result confirming the change in the calf muscle tissue causing muscle damage according to the treatment of red bean 50% ethanol extract (300 mg / kg or 600 mg / kg concentration).
Figure 3 is a result of confirming the change in the calf muscle tissue causing muscle damage according to the treatment of red bean extract (300 mg / kg or 600 mg / kg concentration) treated with protamex.

Hereinafter, the present invention will be described in detail.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Example 1 Preparation of Red Bean Extract

<1-1> methanol extract

The dried red beans were ground with a mixer, and then 100 g of the ground red beans were placed in 1 L of 100% methanol and extracted at room temperature for 3 hours. The extracted sample was filtered under reduced pressure with Whatman No. 2 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to remove the solvent component to obtain a red bean methanol extract.

<1-2> ethanol extract

100% ethanol, 70% ethanol, 50% ethanol, 30% ethanol, 10% ethanol was used as the solvent, except that the extract was carried out at 80 ° C in the same manner as in Example 1-1) Ethanol extract was obtained.

<1-3> ethyl acetate extract

Red bean ethyl acetate extract was obtained in the same manner as in Example 1-1, except that 100% ethyl acetate was used as the solvent.

<1-4> Hexane Extract

Except for using 100% hexane as a solvent was carried out in the same manner as in Example 1-1) to obtain a red bean hexane extract.

<1-5> hydrothermal extract

Except for using water as a solvent was carried out in the same manner as in Example 1-2) to obtain a red bean hot water extract.

<1-6> ultra high pressure extract

The dried red beans were ground with a mixer, and then, 76 mL of 18% ethanol was put in a polyethylene pack, sealed, and extracted using an ultrahigh pressure extractor (Frescal MFP-7000; Mitsubishi Heavy Industries, Tokyo, Japan). Ultra-high pressure extraction conditions were set to 320 MPa extraction time, 5 minutes extraction time. The extracted sample was filtered using Whatman No. 2 filter paper, and the filtered extract was concentrated with a vacuum rotary concentrator to remove the solvent component, thereby obtaining a red bean ultra high pressure extract.

<1-7> supercritical extract

The dried red beans were ground with a mixer, and then 1 g of the ground red beans samples were filled into a sample cartridge and extracted using a supercritical device (SFX 3560, Isco Inc., Lincoln, NE, USA). Supercritical fluid extraction conditions were set to 40 MPa extraction pressure, 50 ℃ extraction temperature, 60 mL / min of the flow rate of supercritical carbon dioxide, extraction time was set to 60 minutes. When the supercritical extraction was completed, the pressure of the extraction device was lowered to release the supercritical fluid state to obtain a red bean supercritical extract.

<1-8> subcritical extract

50 g of crushed red beans were placed in a subcritical water reactor of a subcritical extraction device (Biovan, Gyeonggi, Korea) together with 1 L of water. After sealing, the temperature of the reactor was increased to 200 ° C., and the pressure was increased to 20 MPa. When the temperature of the reactor reached 200 ° C., the temperature was maintained for 20 minutes and extracted. After 20 minutes, the extract was transferred to a storage tank supplied with cooling water and rapidly cooled to 30 ° C., and the supernatant was taken by centrifugation at 3,600 rpm for 30 minutes to separate the floating residue. Red beans subcritical extract was prepared by removing all water using a lyophilizer (ilShin Lab Co. Ltd., Seoul, Korea).

<1-9> Extract Treated with Proteinase

The dried red beans were ground with a mixer, and then 100 g of the ground red beans were placed in 1 L of water and extracted at 80 ° C. for 4 hours. Prior to filtration, 0.1% Alcalase, 0.1% Flavorzyme, 0.1% Neutrase, 0.1% Protamex (Novozymes, Bagsvaerd, Denmark) and 0.1% proteolysis Each of enzyme-NP (Protease-NP; Bioland, Asan, Korea) was reacted with red bean hot water extract for 6 hours under conditions of pH 6.5 and 40 ° C. After 6 hours, the enzyme was inactivated at 90 ° C. for 15 minutes, and after filtration, concentrated with a rotary concentrator (CEP-20S; Okawara Co., Tokyo, Japan). Then 70% ethanol was added and extracted for 12 hours at 45 ℃. After extraction, the resultant was filtered and concentrated by a rotary concentrator (CEP-20S; Okawara Co.), and mixed with hydrothermal extract treated with proteolytic enzyme. The mixture was completely removed with a spray dryer (MSD-60-N; Niro Korea, Chungcheongnam-do, Korea) to prepare a red bean extract treated with proteolytic enzymes.

Example 2 Isolation of Red Bean-Derived Proteins and Peptides

<2-1> Isolation of Red Bean-Derived Protein

After the dried red beans were ground, 1 kg of the ground red beans sample was placed in 1 L hexane and extracted with stirring at room temperature for 4 hours. After removing the hexane extract, water was added to the remaining gourd and left for 30 minutes at pH 9, 25 ℃. The supernatant was collected by centrifugation at 6,000 rpm for 30 minutes, and the supernatant was left to stand again for 30 minutes at a pH of 4.5 and 25 ° C. The precipitated protein was obtained by centrifugation at 6,000 rpm for 30 minutes, and the precipitated protein was dissolved in water and neutralized to remove all water by lyophilizer (ilShin Lab Co. Ltd., Seoul, Korea). Red bean protein was obtained.

<2-2> Preparation of Red Bean Peptides by Alcalase Treatment

Proteolytic enzyme 1% alkalase (Novozymes) was added to the red bean protein obtained in Example <2-1> and subjected to enzymatic reaction at 50 ° C for 6 hours. After 6 hours, the enzyme was inactivated at 90 ° C. for 20 minutes and filtered through Whatman 2 filter paper to remove precipitate. The filtered liquid was removed by using a lyophilizer (ilShin Lab Co. Ltd., Seoul, Korea) to remove all of the water to obtain adzuki beans peptides by alkalase treatment.

<2-3> Preparation of Red Bean Peptides by Flavorzyme Treatment

Flavorzyme-treated red bean peptide was obtained by treating flavozyme instead of alkalase in the same manner as in Example <2-2>.

<2-4> Preparation of Red Bean Peptides by Neutrase Treatment

In the same manner as in Example <2-2>, by treating neutrases (Novozymes) instead of alkalase to obtain a red bean peptide by neutrases treatment.

<2-5> Preparation of Red Bean Peptides by Protamex Treatment

In the same manner as in Example <2-2>, instead of the alkalase was treated with Procamex (Novozymes) to obtain a red bean peptide by the protamex treatment.

<2-6> Preparation of Red Bean Peptides by Protease-NP Treatment

Protease-NP (Bioland, Asan, Korea) instead of alkalase was treated in the same manner as in Example <2-2> to obtain adzuki beans peptides by protease-NP treatment.

Example 3 Evaluation of Activity of Muscle Satellite Cell Pax7 of Red Bean Peptides

Muscle satellite cells were isolated from the hind limbs using 7 week old male rats (C57BL / 6N; Daehan Biolink, Chungcheongbukdo, Korea). Animals were sacrificed via cardiac blood after anesthesia by intraperitoneal injection of 325 mg / kg tribromoethanol (Sigma-aldrich, St Louis, MO, USA). Muscle tissue was removed from the hind limbs and chopped using a razor and dissection scissors, 2.5 U / mL dispase II (Roche Diagnostics, Mannheim, Germany), 2.5 U / mL collagenase B (Roche Diagnostics), 2.5 U / mL Collagenase A (Roche Diagnostics) enzyme was added to the tissue and operated for 1 hour at 37 ° C. to allow the muscle satellite cells to be separated from the muscle tissue. After 1 hour, the suspension was filtered using a 70 μm cell strainer (Falcon, Corning, NY, USA) and a 40 μm cell strainer (Falcon). The collected filter liquid was centrifuged at 33 g for 5 minutes, and then the supernatant was removed. Cells were treated with Ham'F-10 medium (Gibco) consisting of 20% fetal bovine serum (FBS; Gibco, Waltham, Mass., USA), 2.5 ng / mL fibro growth factor (FGF; R & D systems, Minneapolis, MN, USA). After 24 hours of incubation at 37 ° C. and 5% CO ₂ , the cultures were recovered and cultured again for 24 hours in a culture dish coated with collagen (Corning, Corning, NY, USA).

Muscle satellite cells isolated from the hind limbs were dispensed into 6 well plates, and then cultured in medium at 37 ° C. and 5% CO ₂ for 24 hours. The red bean peptide prepared in Example 2-5) was replaced with Dulbecco Modified Eagle Medium (DMEM; Gibco) medium consisting of 5% horse serum (HS; Gibco) containing 50 ug / mL or 100 ug / mL. After 3 days, total RNA was isolated using TRIzol reagent (Takara Bio. Inc., Otsu, Japan). Total RNA isolated was quantified using Nanodrop (NanoDrop 1000; Thermo Fisher Scientific Inc., MA, USA). Quantified 16 μL of RNA was subjected to Reverse Transcriptase Premix (ELPIS-Biotech, Daejeon, Korea) and PCR machine (Gene Amp PCR System 2700; Applied Biosystems, Foster city, CA, USA) It was synthesized by cDNA under the condition of minutes. Of 16 μL of cDNA generated, 4 μL of cDNA, the following specific primers (Bioneer, Daejeon, Korea) and PCR premix (ELPIS-Biotech) were used for 30 seconds at 95 ° C, 1 minute at 60 ° C, and 1 minute at 72 ° C. PCR was repeated 30 times, and the primers used are shown in Table 1 below.

gene SEQ ID NO: Sequence Pax7 One Forward primer 5'-ACTAGAATGGCTTGTATCTGTGGTT-3 ' 2 Reverse primer 5'-TTGAAGTTCCTGCTCCTAAAGTGTA-3 ' β-Actin 3 Forward primer 5'-ACTGTTACTGAGCTGCGTTTTACAC-3 ' 4 Reverse primer 5'-GAGGGACTTCCTGTAACCACTTATT-3 '

The amplified cDNA was isolated by electrophoresis with 1.5% agarose gel, and the cDNA band was identified using G; BOX EF imaging system (Syngene, Cambridge, UK). The results are shown in FIG. 1.

As a result, as shown in Figure 1 it can be seen that the mRNA expression of Pax7 in the muscle satellite cells increases as the red bean peptide is treated. This means that the red bean peptide of the present invention has an excellent ability to promote muscle satellite cell activity through increased expression of Pax7 mRNA, a muscle precursor cell marker, in damaged muscle cells.

Example 4 Evaluation of Impaired Muscle Regeneration Activity of Red Bean Ethanol Extract

As a test animal, a 7-week-old male rat (C57BL / 6N; Young Bio, Chungcheongbuk-do, Korea) was purchased and tested. All animals were raised at Yonsei Laboratory Animal Reaserch Center (YLARC, Seoul, Korea) and the environment was maintained at 23 ± 2 ℃ and 55 ± 10% relative humidity. Before starting the experiment, a total of 20 rats were randomly divided into the normal group, the muscle injury group, the red bean 50% ethanol extract 300 mg / kg concentration group, and the red bean 50% ethanol extract 600 mg / kg concentration group so as to have 5 rats per group. . After adaptation for 1 week, anesthesia was induced by intraperitoneal injection of 325 mg / kg tribromoethanol (Sigma-aldrich). After anesthesia, the right hindlimb gastrocnemius muscle and the right foot of the rat in the muscle injury group and the red bean 50% ethanol extract group were stapled using a skin stapler (Unidos, Chungcheongbuk-do, Korea). This condition was maintained for a week by damaging the muscles with the shim and fixing the right hind legs. A week later, the stapler core fixed in the calf muscle and the sole of the foot was removed, and the red bean 50% ethanol extract prepared in Example 1-2) was again orally administered daily at a concentration of 300 mg / kg or 600 mg / kg for one week. Regeneration was induced. At this time, the normal group and muscle injury group was orally administered saline instead of red beans extract. After the oral administration period, 325 mg / kg tribromoethanol (Sigma-aldrich) was intraperitoneally injected into the experimental animals and euthanized through cardiac blood after anesthesia. After confirming that the heart had stopped, the tissue of the right calf muscle was removed. A part of the tissue of the extracted right calf muscle was removed and fixed with 10% formalin to make a paraffin block. After fixation, hematoxylin & eosin staining was performed to measure muscle recovery in the histological aspect, and the stained tissues were photochemical microscopes equipped with an eXcope T500 camera (DIXI Science, Daejeon, Korea). (CK40; Olymupus, Tokyo, Japan).

As a result, as shown in Figure 2, compared to the normal group in the muscle damage group was confirmed that the nucleus of the satellite cells is located in the center of the muscle fibers, not the edge of the muscle fibers. In contrast, when the red bean extract was administered, the nucleus was located at the edge of the muscle fiber. This means that the red bean 50% ethanol extract of the present invention has an effect of rapidly regenerating muscles.

Example 5 Evaluation of Damaged Muscle Regeneration Activity of Red Bean Extract Treated with Protease

Evaluation of muscle regeneration activity in the same manner as in Example 4, except that 300 mg / kg and 600 mg / kg oral administration of the protamex-treated red bean extract prepared in Example 1-9) It was.

As a result, as shown in Figure 3, compared to the normal group, muscle nuclei in the muscle damage group was confirmed that the center of the muscle fibers, not the edge of the muscle fibers. On the other hand, when the red bean extract treated with proteolytic enzymes was administered, the nucleus was located at the edge of the muscle fiber. This means that the red bean extract treated with the proteolytic enzyme of the present invention has an effect of rapidly recovering muscles.

Hereinafter, an example of the preparation of the composition containing the red bean extract prepared in Example 1 of the present invention, but the present invention is not intended to limit it, it is intended to explain in detail only.

Formulation Example 1 Preparation of Powder

Red Bean Extract 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients were mixed and filled in airtight cloth to prepare a powder.

Formulation Example 2: Preparation of Tablet

Red Bean Extract 10 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

Formulation Example 3: Preparation of Capsule

Red Bean Extract 10 mg

3 mg of microcrystalline cellulose

Lactose carb 14.8 mg

Magnesium Stearate 0.2mg

After mixing the above components, it was filled in gelatin capsules according to the conventional method for producing capsules to prepare a capsule.

Formulation Example 4 Preparation of Injection

Red Bean Extract 10 mg

Mannitol 180 mg

Sterile distilled water for injection 2974 mg

Monohydrogen phosphate 26 mg

After mixing the above components, it was prepared in the above ingredient content per ampoules (2 mL) according to the conventional method for preparing injections.

Formulation Example 5 Preparation of Liquid

Red Bean Extract 10 mg

Isomerized sugar 10 mg

Mannitol 5 mg

Purified water

Lemon flavor

The above components are dissolved in purified water according to a conventional manufacturing method, dissolved in purified water, lemon juice is added in an appropriate amount, adjusted to 100 mL by adding purified water, and sterilized to fill a brown bottle to prepare a liquid formulation.

Formulation Example 6 Preparation of Health Functional Food

Red Bean Extract 10 mg

Vitamin Mixture

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B ₁ 0.13 mg

Vitamin B ₂ 0.15mg

Vitamin B ₆ 0.5 mg

0.2 μg of vitamin B ₁₂

Vitamin C 10 mg

10 μg biotin

Nicotinamide 1.7 mg

50 μg folic acid

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium phosphate monobasic 15 mg

Dicalcium Phosphate 55 mg

Potassium Citrate 30 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a composition suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation Example 7 Preparation of Health Beverage

Red Bean Extract 10 mg

15 g of vitamin C

100 g of vitamin E (powder)

Iron lactate 19.75 g

3.5 g of zinc oxide

3.5 g of nicotinic acid amide

0.2 g of vitamin A

Vitamin B ₁ 0.25 g

0.3 g of vitamin B ₂

Purified Water Quantification

After mixing the above components according to a conventional healthy beverage manufacturing method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and refrigerated Used to prepare the healthy beverage composition of the invention.

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

Hereinafter, the preparation example of the cosmetic composition containing the red bean extract of the present invention will be described, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

Preparation Example 1: Nutrients (Milk Lotion)

Red beans extract 2.0% by weight of the present invention

Squalane 5.0 wt%

Beeswax 4.0 wt%

Polysorbate60 1.5 wt%

Sorbanthesquioleate 1.5 wt%

0.5% by weight of liquid paraffin

Caprylic / Capric Triglycerides 5.0 wt%

Glycerin 3.0 wt%

Butylene Glycol 3.0 wt%

Propylene Glycol 3.0 wt%

Carboxy vinyl polymer 0.1 wt%

0.2% by weight of triethanolamine

Preservatives, colorings, flavors

Purified water to 100% by weight

Although the blending ratio is a composition that is relatively suitable for nutritional longevity in a preferred embodiment, the blending ratio may be arbitrarily modified, it can be prepared according to the manufacturing method in the general cosmetic field.

Preparation Example 2 Softener (Skin Lotion)

Red beans extract 2.0% by weight of the invention

Glycerin 3.0 wt%

Butylene glycol 2.0% by weight

Propylene Glycol 2.0 wt%

Carboxy vinyl polymer 0.1 wt%

PEG 12 nonylphenylether 0.2% by weight

Polysorbate 80 0.4% by weight

Ethanol 10.0 wt%

Triethanolamine 0.1% by weight

Preservatives, colorings, flavors

Purified water to 100% by weight

Although the above-mentioned compounding ratio is mixed and formulated in a preferred embodiment with a component suitable for a relatively softening longevity, the compounding ratio may be arbitrarily modified, and can be prepared according to the manufacturing method in the general cosmetic field.

Preparation Example 3 Nutrition Cream

Red beans extract 2.0% by weight of the present invention

Polysorbate60 1.5 wt%

Solbitan Sesquioleate 0.5% by weight

PEG60 Cured Castor Oil 2.0% by weight

10% by weight of liquid paraffin

Squalane 5.0 wt%

Caprylic / Capric Triglyceride 5.0 wt%

Glycerin 5.0 wt%

Butylene glycol 3.0% by weight

Propylene Glycol 3.0 Weight%

Triethanolamine 0.2% by weight

Preservative

Pigment amount

Spices

Purified water to 100% by weight

Although the above-mentioned compounding ratio is mixed with a component suitable for nourishing cream in a preferred embodiment, the compounding ratio may be arbitrarily modified, and can be prepared according to a conventional manufacturing method in the cosmetic field.

Preparation Example 4 Massage Cream

Red beans extract 1.0% by weight of the present invention

Beeswax 10.0 weight%

Polysorbate60 1.5 wt%

PEG 60 Cured Castor Oil 2.0% by weight

Sorbanthesquioleate 0.8 wt%

40.0% by weight of liquid paraffin

Squalane 5.0 wt%

Caprylic / Capric Triglyceride 4.0 wt%

Glycerin 5.0 wt%

Butylene glycol 3.0% by weight

Propylene Glycol 3.0 Weight%

Triethanolamine 0.2% by weight

Preservatives, colorings, flavors

Purified water to 100% by weight

Although the above-mentioned compounding ratio is mixed with a component suitable for a massage cream in a preferred embodiment, the compounding ratio may be arbitrarily modified, and can be prepared according to a conventional manufacturing method in the cosmetic field.

Preparation Example 5 Pack

Red beans extract 1.0% by weight of the invention

Polyvinyl alcohol 13.0 wt%

Sodium Carboxymethylcellulose 0.2% by weight

Glycerin 5.0 wt%

Allantoin 0.1 wt%

Ethanol 6.0 wt%

PEG 12 nonylphenylether 0.3 wt%

Polysorbate60 0.3 wt%

Preservatives, colorings, flavors

Purified water to 100% by weight

Although the above-mentioned compounding ratio is mixed with a component suitable for a pack in a preferred embodiment, the compounding ratio may be arbitrarily modified, and can be prepared according to a manufacturing method in the general cosmetic field.

Preparation Example 6 Gel

0.5% by weight red bean extract of the present invention

Ethylenediamine sodium acetate 0.05% by weight

Glycerin 5.0 wt%

Carboxy vinyl polymer 0.3 wt%

Ethanol 5.0 wt%

PEG 60 Cured Castor Oil 0.5% by weight

0.3% by weight of triethanolamine

Preservatives, colorings, flavors

Purified water to 100% by weight

Although the above-mentioned compounding ratio is mixed with a component suitable for a gel in a preferred embodiment, the compounding ratio may be arbitrarily modified, and can be prepared according to a manufacturing method in the general cosmetic field.

Although the compounding ratio is a relatively suitable composition for the cosmetic composition is mixed in a preferred embodiment, it can be applied to a variety of cosmetics, including other color cosmetics, according to the efficacy that can be applied to a thin coating on the human body, that is, It can be used for manufacturing as ointment, and the compounding ratio may be arbitrarily modified according to regional and national preferences such as demand hierarchy, demand country, and usage.

The above description of the present invention is intended for illustration, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive.

As described above, peptides isolated from red beans not only increased the mRNA of Pax7, but also the red beans extract quickly recovered muscles, so that the nucleus of muscle satellite cells was located at the edge of muscle fibers as normal. Therefore, the red bean extract of the present invention may activate the satellite cells, alleviate the decrease in muscle function due to muscle damage, and may promote recovery from muscle damage, preventing or improving muscle damage, treating or regenerating damaged muscle. It can be usefully used for promotion.

<110> NewTree Co., Ltd.          Industry-Academic Cooperation Foundation, Yonsei University <120> Composition for preventing, improving or treating muscular damage          comprising Vigna angularis extract <130> NP17-0119P <150> 10-2017-0168646 <151> 2017-12-08 <160> 4 <170> KoPatentIn 3.0 <210> 1 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Pax7 primer forward <400> 1 actagaatgg cttgtatctg tggtt 25 <210> 2 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Pax7 primer reverse <400> 2 ttgaagttcc tgctcctaaa gtgta 25 <210> 3 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> beta-Actin primer forward <400> 3 actgttactg agctgcgttt tacac 25 <210> 4 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> beta-Actin primer reverse <400> 4 gagggacttc ctgtaaccac ttatt 25

Claims (11)

Muscle strain, muscle rupture, muscle tearing, contusion, sprain and rotator cuff syndrome, including red bean extract treated with proteolytic enzyme as an active ingredient Pharmaceutical composition for preventing or treating motor-induced muscle injury selected from the group consisting of (roator cuff syndrome).
The composition of claim 1, wherein the red bean extract is an extract by at least one solvent selected from the group consisting of water, an organic solvent having 1 to 6 carbon atoms, a subcritical fluid, a supercritical fluid, and mixtures thereof.
The composition of claim 1, wherein the red bean extract is obtained by treating and extracting red beans at an ultrahigh pressure for 1 to 24 hours at a temperature of 40 to 90 ° C. at a pressure of 100 to 1000 MPa.
The method according to claim 1, wherein the proteolytic enzyme is Alcalase, Flavorzyme, Neutrase, Protamex and Protease-NP At least one composition selected from the group consisting of.
delete delete Muscle strain, muscle rupture, muscle tearing, contusion, sprain and rotator cuff syndrome, including red bean extract treated with proteolytic enzyme as an active ingredient (roator cuff syndrome) food composition for preventing or improving muscle damage selected from the group consisting of.
Muscle strain, muscle rupture, muscle tearing, contusion, sprain and rotator cuff syndrome, including red bean extract treated with proteolytic enzyme as an active ingredient (roator cuff syndrome) cosmetic composition for preventing or improving muscle damage selected from the group consisting of.
Muscle strain, muscle rupture, muscle tearing, contusion, sprain and rotator cuff syndrome, including red bean extract treated with proteolytic enzyme as an active ingredient Pharmaceutical composition for promoting regeneration of damaged muscles by exercise-induced muscle injury selected from the group consisting of (roator cuff syndrome).
Muscle strain, muscle rupture, muscle tearing, contusion, sprain and rotator cuff syndrome, including red bean extract treated with proteolytic enzyme as an active ingredient (Roator cuff syndrome) A food composition for promoting regeneration of damaged muscles by exercise-induced muscle damage selected from the group consisting of.
Muscle strain, muscle rupture, muscle tearing, contusion, sprain and rotator cuff syndrome, including red bean extract treated with proteolytic enzyme as an active ingredient Cosmetic composition for promoting regeneration of damaged muscles by exercise-induced muscle damage selected from the group consisting of (roator cuff syndrome).

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