KR102065357B1 - Manufacturing method of rice bran fermented beverage composition - Google Patents
Manufacturing method of rice bran fermented beverage composition Download PDFInfo
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- KR102065357B1 KR102065357B1 KR1020180089549A KR20180089549A KR102065357B1 KR 102065357 B1 KR102065357 B1 KR 102065357B1 KR 1020180089549 A KR1020180089549 A KR 1020180089549A KR 20180089549 A KR20180089549 A KR 20180089549A KR 102065357 B1 KR102065357 B1 KR 102065357B1
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- rice
- extract
- lactic acid
- weight
- acid bacteria
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- 235000009566 rice Nutrition 0.000 title claims abstract description 151
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
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- 240000007594 Oryza sativa Species 0.000 title 1
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 94
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- 235000019421 lipase Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C11/00—Milk substitutes, e.g. coffee whitener compositions
- A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
- A23C11/10—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/12—Replacer
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
Description
본 발명은 쌀눈발효음료 조성물 제조방법에 관한 것으로, 보다 상세하게는 미강에서 분리한 쌀눈에서 추출한 쌀눈 추출물을 유산균으로 발효시켜 쌀눈발효음료 조성물을 제조하는 쌀눈발효음료 조성물 제조방법에 관한 것이다.The present invention relates to a method for producing a fermented beverage composition for rice bran, and more particularly, to a method for producing a fermented beverage composition for brewing a fermented beverage composition by fermenting a rice berry extract extracted from rice bran isolated from rice bran with lactic acid bacteria.
쌀은 우리나라뿐만이 아니라 전 세계의 50 % 가 넘는 인구의 주식으로 사용되는 중요한 곡물이다. 쌀은 한국인의 주요 에너지원으로서 하루에 필요한 에너지의 30~40%를 쌀에서 섭취하는데, 쌀눈과 쌀겨를 중심으로 탄수화물, 단백질, 지방, 식이섬유, 미네랄 등 10여가지의 영양성분이 존재한다. 쌀의 많은 영양소가 현미와 쌀눈에 존재하며, 쌀은 일반적으로 왕겨가 20 % 내외, 쌀겨가 3 % 내외, 쌀눈 2% 내외로 구성되어 있다. Rice is an important grain that is used not only in Korea but also as a staple food for over 50% of the world's population. Rice is the main energy source for Koreans and consumes 30 ~ 40% of the energy needed per day from rice. There are 10 kinds of nutrients such as carbohydrates, proteins, fats, dietary fiber, and minerals. Many of the nutrients in rice are present in brown rice and rice eyes, and rice generally consists of about 20% rice husk, about 3% rice bran, and about 2% rice bran.
쌀을 주식으로 하는 국내에서 벼 생산량이 최근 약 552만톤 정도이며 도정시 발생하는 부산물인 왕겨는 약 118만톤, 미강은 약 47만톤, 쌀눈은 약 20만톤 내외로 발생되는 것으로 추정된다. 특히 쌀눈은 다양한 영양적 가치가 있는데도 불구하고 대부분이 미강에 혼입되어 사료로 사용되고 있다.In Korea, rice production is estimated at about 5.44 million tons, and rice production is estimated at about 1.18 million tons of rice hull, about 470,000 tons of rice bran, and about 200,000 tons of rice snow. In particular, although rice bran has various nutritional values, most of them are mixed with rice bran and used as feed.
쌀 구조 중 2%를 차지하는 쌀눈은 배유와 비교하였을 때 양질의 단백질과 20% 이상의 지방, 무기질 및 비타민 A, B, E등이 함유되어 있다. 이런 영양적 가치 때문에 쌀눈 기름, 쌀눈 비누, 숙취 음료 등의 제품이 출시되었으나 쌀겨로부터 쌀눈의 순수 분리가 어렵고 도정 후 lipase, lipoxydase의 작용으로 산패가 급격히 일어나, 쌀눈 고유의 곡물취로 인하여 쌀눈을 소비자들이 직접 섭취하기에는 어려움이 있었다.Rice bran, which accounts for 2% of the rice structure, contains more than 20% fat, minerals, vitamins A, B, and E, as well as high-quality protein. Due to this nutritional value, products such as rice flakes oil, rice flakes soap, and hangover drinks have been released, but it is difficult to purely separate rice flakes from rice bran, and rancidity occurs rapidly due to the action of lipase and lipoxydase. Difficult to eat directly.
이에, 등록특허 제10-1542667에서는 미강, 쌀눈 및 아마씨를 이용하여 쌀눈을 발효식품으로 섭취하고 있다.Thus, in Patent No. 10-1542667, rice bran is consumed as fermented food using rice bran, rice flakes, and flaxseed.
또한, 우유는 전통적으로 영양공급 또는 식사 대체용, 식품원료로서 널리 사용되고 있는 식품이다. 특히, 어린이나 노인, 회복기 환자등 영양이 부족하기 쉬운 사람들에게는 효과적인 영양공급식품으로서 이용되고 있으나 개중에는 유당을 분해이용할 수 없는 유당불내증을 가지고 있는 사람들이 있어 이런 사람들은 우유를 섭취할 경우 복부팽만감이나 설사 등의 이상반응을 보여 우유를 섭취할 수 없었다. 그래서 이렇게 우유 섭취를 못하는 사람들을 위해, 국제적으로 유당이 없는 새로운 형태의 우유를 만들기 시작했는데, 대표적인 것이 콩으로 만든 두유이다. 두유는 전통적으로 많이 제조되어 음용되어 오고 있으며, 국내 뿐만 아니라 전 세계적으로도 널리 이용되고 있으나, 콩의 알러지 유발 문제와 GMO 콩 문제 등으로 인해 최근에는 콩이 아닌 다른 식물성 식량자원으로부터 우유대체품을 만들려는 시도가 계속 있어왔다.In addition, milk is a food that has traditionally been widely used as a food source for nutrition or meal replacement. In particular, it is used as an effective nutritional food for people who are poor in nutrition such as children, the elderly, and recovery patients, but some people have lactose intolerance that cannot break down lactose. I could not consume milk because of adverse reactions such as diarrhea and diarrhea. So for those who can't drink milk, I started making a new type of milk that's lactose-free internationally, soy milk made from soybeans. Soy milk has traditionally been manufactured and consumed, and is widely used not only in Korea but also throughout the world.However, due to allergic problems of soybeans and GMO soybeans, we have recently tried to make milk substitutes from vegetable food sources other than soybeans. Has been trying.
따라서, 본 발명은 상기 문제를 해결하기 위해 안출된 것으로, 항산화 효능을 포함하고, 유산균을 이용하여 발효된 쌀눈발효 추출물 및 쌀 우유를 혼합하여 우유 성분을 포함하지 않는 쌀눈발효음료 조성물 제조방법을 제공하고자 한다.Therefore, the present invention has been made to solve the above problems, including the antioxidant effect, by mixing the fermented rice snow fermentation extract and rice milk fermented using lactic acid bacteria provides a method for producing a rice snow fermented beverage composition containing no milk ingredients I would like to.
상기 문제를 해결하기 위하여 본 발명은, (a)미강에서 분리한 쌀눈 및 물을 혼합한 후 80~100℃에서 2~6시간 추출한 쌀눈추출물을 제조하고, 상기 쌀눈추출물을 굴절 당도계 기준으로 1~10brix가 될 때까지 진공 농축하여 쌀눈추출농축액을 제조하는 단계; (b)상기 쌀눈추출농축액에 식물성 유산균을 접종한 후, 발효조에 첨가하여 30~40℃에서 15~25시간 동안 발효 배양하여 쌀눈추출 유산균 발효액을 제조하는 단계; 및 (c)상기 쌀눈추출 유산균발효액에 쌀 우유를 혼합하여 70~90℃에서 30~90분 동안 교반하여 쌀눈발효음료 조성물을 제조하는 단계;를 포함하는 쌀눈발효음료 조성물 제조방법을 제공한다.In order to solve the above problems, the present invention, (a) after mixing the rice snow and water separated from the rice bran to prepare a rice snow extract extracted for 2-6 hours at 80 ~ 100 ℃, the
또한, 상기 (a)단계는 상기 쌀눈 및 물의 혼합 비율이 상기 쌀눈 100중량부 대비 상기 물 500~1500중량부이고, 상기 (b)단계는 상기 식물성 유산균이 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주이고, 상기 쌀눈추출농축액 및 상기 혼합균주의 접종비율은 상기 쌀눈추출농축액 100중량부 대비 상기 혼합균주 1~5중량부이고, 상기 (c)단계는 상기 쌀눈추출 유산균 발효액 및 상기 쌀 우유의 혼합 비율이 상기 쌀눈추출 유산균 발효액 100중량부 대비 상기 쌀 우유 500~1000중량부인 것을 특징으로 하는 쌀눈발효음료 조성물 제조방법을 제공한다.In addition, the (a) step is the mixing ratio of the rice eye and water is 500 to 1500 parts by weight of water compared to 100 parts by weight of the rice eye, the step (b) is the plant lactic acid bacteria Lactobacillus Bulgaricus ( Lactobacillus bulgaricus ) and Streptococcus thermophilus ( Streptococcus thermophilus ) is a mixed strain, the inoculation ratio of the rice eye extract concentrate and the mixed strain is 1 to 5 parts by weight of the mixed strain compared to 100 parts by weight of the rice eye extract concentrate, step (c) The rice lance extract lactic acid bacteria fermentation broth and the ratio of the rice milk provides a method for producing a fermented beverage composition, characterized in that the rice milk 500 to 1000 parts by weight relative to 100 parts by weight of the rice lance extract lactic acid bacteria fermentation broth.
또한, 상기 (c)단계는 상기 쌀눈추출 유산균발효액 및 상기 쌀 우유 혼합물에 이소말토올리고당, 액산과당 또는 결정과당, 구연산, 천연 과즙 및 천연 과일향을 혼합하는 것을 특징으로 하는 쌀눈발효음료 조성물 제조방법을 제공한다.In addition, the step (c) is a rice snow fermented beverage composition, characterized in that the mixture of isomaltooligosaccharide, liquid acid fructose or crystalline fructose, citric acid, natural fruit juice and natural fruit flavor in the rice extract lactic acid bacteria fermentation broth and the rice milk mixture Provide a method.
본 발명에 따르면, 미강에서 분리된 쌀눈에서 추출한 쌀눈 추출물을 유산균을 이용하여 발효시킴으로써, 쌀눈 고유의 곡물취를 제거하여 항산화 효능을 가지고 있는 쌀눈의 영양분을 그대로 섭취할 수 있고, 기존 우유를 사용하는 식물성 발효음료와는 달리 쌀 우유를 사용함으로써, 유당불내증을 가지고 있는 사람들도 소화 불량 없이 섭취가능한 쌀눈발효음료 조성물을 제조할 수 있다. According to the present invention, by fermenting the rice eye extract extracted from the rice bran separated from the rice bran using lactic acid bacteria, it is possible to ingest the nutrients of the rice eye having antioxidant properties by removing the grain odor inherent in the rice bran, and using the existing milk Unlike plant fermented beverages, by using rice milk, people with lactose intolerance can prepare a fermented beverage composition for rice eye consumption without indigestion.
도 1은 본 발명의 실시예에서 제조된 쌀눈발효음료 조성물의 항산화 효능을 측정하기 위한 라디칼 소거능을 측정한 그래프이고,
도 2는 본 발명의 실시예에서 제조된 쌀눈발효음료 조성물의 항산화 효능을 측정하기 위한 총 폴리페놀의 함량을 측정한 그래프이다.Figure 1 is a graph measuring the radical scavenging ability for measuring the antioxidant efficacy of the rice eye fermented beverage composition prepared in the embodiment of the present invention,
Figure 2 is a graph measuring the content of the total polyphenols for measuring the antioxidant efficacy of the rice frost fermented beverage composition prepared in the embodiment of the present invention.
이하 바람직한 실시예를 통하여 본 발명을 상세히 설명하기로 한다. 이에 앞서, 본 명세서 및 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니 되며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념을 적절하게 정의할 수 있다는 원칙에 입각하여, 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다. 따라서, 본 명세서에 기재된 실시예의 구성은 본 발명의 가장 바람직한 일 실시예에 불과할 뿐이고 본 발명의 기술적 사상을 모두 대변하는 것은 아니므로, 본 출원시점에 있어서 이들을 대체할 수 있는 다양한 균등물과 변형 예들이 있을 수 있음을 이해하여야 한다. 또한, 명세서 전체에서, 어떤 부분이 어떤 구성요소를 "포함"한다고 할 때, 이는 특별히 반대되는 기재가 없는 한, 다른 구성요소를 제외하는 것이 아니라 다른 구성요소를 더 포함할 수 있음을 의미한다.Hereinafter, the present invention will be described in detail with reference to preferred embodiments. Prior to this, terms or words used in the specification and claims should not be construed as having a conventional or dictionary meaning, and the inventors should properly explain the concept of terms in order to best explain their invention in the best way possible. Based on the principle that it can be defined, it should be interpreted as meaning and concept corresponding to the technical idea of the present invention. Therefore, the configuration of the embodiments described herein is only one of the most preferred embodiments of the present invention and does not represent all of the technical idea of the present invention, various equivalents and modifications that can replace them at the time of the present application It should be understood that there may be In addition, throughout the specification, when a part is said to "include" a certain component, it means that it may further include other components, not to exclude other components, unless otherwise stated.
본 발명자들은, 쌀눈의 곡물취를 해결하고 쌀눈의 영양분을 손실없이 섭취하기 위하여 예의 노력을 한 결과, 쌀눈으로부터 쌀눈 추출물을 추출한 후 유산균 중 락토바실러스 불가리쿠스(Lactobacillus bulgaricus) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주를 이용하여 상기 쌀눈 추출물을 발효시키는 경우, 쌀눈 고유의 곡물취를 제거할 수 있어 소비자가 거부감 없이 섭취할 수 있고, 쌀눈 추출물을 이용함에 따라 쌀눈 고유의 영양분의 섭취가 가능함을 확인하고 본 발명에 이르게 되었다.The present inventors have made diligent efforts to solve the grain odor of rice bran and consume the nutrients of the rice bran without loss, and after extracting the rice bran extract from the rice bran, Lactobacillus bulgaricus and When fermenting the rice flakes extract using a mixed strain of Streptococcus thermophilus , it is possible to remove the grain odor inherent in the rice flakes, which can be consumed by consumers without objection. It was confirmed that the intake of nutrients has led to the present invention.
따라서, 본 발명은 (a)미강에서 분리한 쌀눈 및 물을 혼합한 후 80~100℃에서 2~6시간 추출한 쌀눈추출물을 제조하고, 상기 쌀눈추출물을 굴절 당도계 기준으로 1~10brix가 될 때까지 진공 농축하여 쌀눈추출농축액을 제조하는 단계; (b)상기 쌀눈추출농축액에 식물성 유산균을 접종한 후, 발효조에 첨가하여 30~40℃에서 15~25시간 동안 발효 배양하여 쌀눈추출 유산균 발효액을 제조하는 단계; 및 (c)상기 쌀눈추출 유산균발효액에 쌀 우유를 혼합하여 70~90℃에서 30~90분 동안 교반하여 쌀눈발효음료 조성물을 제조하는 단계;를 포함하는 쌀눈발효음료 조성물 제조방법에 관한 것이다.Therefore, the present invention (a) after mixing rice snow and water separated from rice bran to prepare a rice snow extract extracted for 2-6 hours at 80 ~ 100 ℃, until the rice snow extract is 1 ~ 10brix on the basis of refractive sugar meter Concentrating in vacuo to prepare a rice eye extract concentrate; (b) inoculating the vegetable lactic acid bacteria to the rice extract extract, and then added to the fermenter to fermentation culture for 15 to 25 hours at 30 ~ 40 ℃ to produce a rice eye extract lactic acid bacteria fermentation broth; It relates to a method for producing a fermented beverage composition comprising; and (c) mixing the rice milk in the fermented lactic acid bacteria fermentation broth and stirring for 30 to 90 minutes at 70 ~ 90 ℃.
상기 미강에서 분리한 쌀눈은 고유의 저 알레르기성 단백질을 14%나 포함하고 있으며, 분자량이 작은 편이므로 유산균 등 미생물 발효 시 질소원으로 이용하기에 적당한 품질을 가지고 있으며, 쌀눈 내 저 알레르기성 단백질을 활용하여 발효유를 제조할 경우 저자극성 어린이 영양에 좋은 발효유를 생산할 수 있다.The rice bran isolated from the rice bran contains 14% of its own low allergenic protein, and has a low molecular weight, so it has a quality suitable for use as a nitrogen source when fermenting microorganisms such as lactic acid bacteria, and utilizes a low allergenic protein in rice bran. In the case of fermented milk, it is possible to produce fermented milk that is good for hypoallergenic children.
또한, 쌀눈은 콜레스테롤을 줄이는 옥타코사놀, 항산화 기능의 알파토코페롤, 아토피에 좋은 리놀렌산, 장을 튼튼하게 하는 베타시스테롤 등을 포함하고 있다.Rice eyes also contain octacosanol to reduce cholesterol, alpha tocopherol with antioxidant function, linolenic acid good for atopy, and beta cysterol to strengthen the intestines.
상기 발효란, 효모나 세균 따위의 미생물이 유기 화합물을 분해하여 알코올류, 유기산류, 이산화탄소 따위를 생기게 하는 작용. 좁은 뜻으로는 산소가 없는 상태에서 미생물이 탄수화물을 분해하여 에너지를 얻는 작용을 말한다. The fermentation is a function in which microorganisms such as yeasts and bacteria decompose organic compounds to produce alcohols, organic acids, carbon dioxide and the like. In a narrow sense, it refers to the action of microbes decomposing carbohydrates and obtaining energy in the absence of oxygen.
상기 쌀 우유는 쌀을 이용하여 만든 것으로 쌀 우유는 에너지를 주고, 소화에도 좋고 세포를 성장하게 하고 노화를 늦춰주며, 유당불내증을 가지고 있는 사람이 섭취 시에도 소화불량에 걸리지 않는다.The rice milk is made using rice as rice milk gives energy, is good for digestion, makes cells grow and slows aging, and people with lactose intolerance do not suffer from indigestion even when ingested.
상기 (a)단계에서 상기 쌀눈 및 물의 혼합비율은 쌀눈 100중량부 대비 물 500~1500중량부일 수 있고, 바람직하게는 700~1300중량부일 수 있고, 더욱 바람직하게는 900~1100중량부일 수 있다.In the step (a), the mixing ratio of the rice eye and water may be 500 to 1500 parts by weight of water, preferably 700 to 1300 parts by weight, and more preferably 900 to 1100 parts by weight, relative to 100 parts by weight of rice eyes.
또한, 추출 시 온도는 바람직하게 85~95℃이고, 시간은 바람직하게 3~5시간이다. In addition, the temperature at the time of extraction is preferably 85 to 95 ° C, and the time is preferably 3 to 5 hours.
상기 쌀눈 및 물의 혼합비율이 쌀눈 100중량부 대비 물 500중량부 미만이고, 추출 시 온도가 100℃를 초과하거나, 추출 시간이 5시간을 초과하는 경우에는 쌀눈에서 추출되는 영양소의 손실이 일어날 수 있고, 소비자가 섭취 시 맛에 대한 만족도가 낮을 수 있고, 상기 쌀눈 및 물의 혼합비율이 쌀눈 100중량부 대비 물 1500중량부를 초과하고, 추출 시 온도가 80℃ 미만이거나, 추출 시간이 3시간 미만인 경우에는 쌀눈에서 영양소가 충분히 추출되지 않을 수 있고, 소비자가 섭취 시 맛에 대한 만족도가 낮을 수 있다.When the mixing ratio of the rice flakes and water is less than 500 parts by weight of water to 100 parts by weight of rice flakes, and the extraction temperature exceeds 100 ℃, or the extraction time exceeds 5 hours, loss of nutrients extracted from the rice flakes may occur , If the consumer is low in satisfaction with the taste, the mixing ratio of the rice eye and water is more than 1500 parts by weight of water compared to 100 parts by weight of rice, and when the extraction temperature is less than 80 ℃ or extraction time is less than 3 hours Nutrients may not be extracted enough from rice eyes, and consumers may be less satisfied with the taste when ingested.
또한, 상기 진공 농축 시, 진공농축기(경서산업, Cosmos660)를 이용하여 50~70℃, 바람직하게 55~65℃에서, -0.4~-0.7bar, 바람직하게 -0.5~-0.6bar의 압력으로 상기 쌀눈추출 농축액의 당도가 굴절당도계 기준으로 1~10birx, 바람직하게 3~7brix가 될 때까지 진공 농축할 수 있다.In addition, when the vacuum concentration, using a vacuum concentrator (Gyeongseo industry, Cosmos660) at 50 ~ 70 ℃, preferably 55 ~ 65 ℃, -0.4 ~ -0.7 bar, preferably -0.5 ~ -0.6 bar at the pressure The sugar content of the rice eye extract concentrate may be concentrated in vacuo until the sugar content becomes 1-10birx, preferably 3-7brix, based on the refractive sugar meter.
상기 진공 농축 후, 제조된 쌀눈추출농축액을 교반기에 넣고 4000~6000rpm, 바람직하게 4500~5500rpm으로, 5~15분 동안, 바람직하게 7~13분 동안 균질한 다음, 90~100℃, 바람직하게 92~98℃에서 10~20분 동안, 바람직하게 13~17분 동안 살균 공정을 진행할 수 있다.After the vacuum concentration, put the prepared rice eye extract concentrate in a stirrer at 4000 ~ 6000rpm, preferably 4500 ~ 5500rpm, homogeneous for 5-15 minutes, preferably 7-13 minutes, and then 90 ~ 100 ℃, preferably 92 The sterilization process may be performed at ˜98 ° C. for 10-20 minutes, preferably for 13-17 minutes.
상기 (b)단계에서 상기 식물성 유산균은 자연계에 널리 존재하며 탄수화물을 혐기적으로 이용하여 유산을 생산하고, 사람이나 동물의 장내에 존재할 뿐 아니라, 다양한 채소와 과일에서도 발견되며 우리나라의 김치나 요구르트와 같은 발효식품에서 그 발효과정에 중요한 역할을 담당한다.In the step (b), the vegetable lactic acid bacteria are widely present in nature and produce lactic acid by using carbohydrates anaerobically, and exist in the gut of humans and animals, as well as in various vegetables and fruits. In the same fermented foods play an important role in the fermentation process.
또한, 이들 유산균은 장내로 유입된 후 장내 상피세포에 착생하게 되어 유해 미생물의 장 정착을 방지하고 항균 물질을 분비함으로써 유해 미생물의 생육을 억제하고 설사와 변비를 개선할 뿐만 아니라, 면역활성 증진, 항암작용, 혈청 콜레스테롤 저하 등의 작용을 수행한다 또한, 일반적으로 이들 유산균은 직접 혹은 간접적으로 식품에 첨가되어 이들의 대사 산물인 유산에 의해 식품의 저장성을 향상시키며 식품의 향미와 조직을 개선한다고 알려져 있다. In addition, these lactic acid bacteria enters the intestinal epithelial cells and enters the intestinal epithelial cells, preventing the intestinal settlement of harmful microorganisms and secreting antimicrobial substances, thereby inhibiting the growth of harmful microorganisms and improving diarrhea and constipation, as well as enhancing immune activity, It is also known that these lactic acid bacteria are added directly or indirectly to foods to improve the shelf life of foods and improve the flavor and texture of foods by their metabolites, lactic acid bacteria. have.
또한, 본 발명에서는 상기 유산균으로 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주를 사용할 수 있고, 상기 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주는 Chr.Hansen사(YF-L811, Denmark)에서 구매할 수 있다.In the present invention, the lactobacillus Lactobacillus vulgaricus ( Lactobacillus bulgaricus ) and Streptococcus Thermo filler's can be used in a mixed culture (Streptococcus thermophilus), the Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus) and Streptococcus Thermo mixed culture of the filler's (Streptococcus thermophilus) is It is available from Chr. Hansen (YF-L811, Denmark).
또한, 상기 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주는 MRS broth배지에서 배양될 수 있고, MRS broth배지는 Dextrose 20g, Peptic digest of animal tissue 10g, Beef extract 10g, Sodium acetate 5g, Disodium phosphate 2g, Yeast extract 5g, Tween 80 1g, Magnesium sulfate 0.1g, Manganese sulfate 0.05g 으로 이루어져 있다. In addition, the Lactobacillus bulgaricus ( Lactobacillus bulgaricus ) and Streptococcus Thermo mixed culture of the pillar's (Streptococcus thermophilus) may be cultured in a MRS broth medium, MRS broth medium Dextrose 20g, Peptic digest of animal tissue 10g, Beef extract 10g, Sodium acetate 5g, Disodium phosphate 2g, Yeast extract It consists of 5g, Tween 80 1g, 0.1g Magnesium sulfate and 0.05g Manganese sulfate.
상기 (b)단계에서 상기 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주는 상기 쌀눈추출 농축액 100중량부 대비 1~5중량부가 접종될 수 있고, 바람직하게는 2~4중량부가 접종될 수 있다.In the step (b) the Lactobacillus vulgaricus ( Lactobacillus bulgaricus ) and Mixed strains of Streptococcus thermophilus ( Streptococcus thermophilus ) may be inoculated 1 to 5 parts by weight relative to 100 parts by weight of the rice extract extract, preferably 2 to 4 parts by weight may be inoculated.
상기 (b)단계에서 발효 시 온도는 바람직하게 33~37℃이고, 시간은 바람직하게 16~20시간이다. In the step (b), the temperature during fermentation is preferably 33 to 37 ° C, and the time is preferably 16 to 20 hours.
상기 (b)단계에서 상기 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주가 쌀눈추출 농축액 100중량부 대비 1중량부 미만이거나, 온도가 30℃ 미만인 경우, 또는 발효 시간이 15시간 미만인 경우에는 발효 공정이 충분히 진행되지 않아 쌀눈 고유의 곡물취가 제거되지 않을 수 있고, 상기 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주가 쌀눈추출 농축액 100중량부 대비 5중량부를 초과하거나, 상기 발효 공정에서 온도가 40℃를 초과하는 경우, 또는 발효 시간이 25시간을 초과하는 경우, 추가 발효 공정으로 인한 냄새가 발생할 수 있다.In the step (b) the Lactobacillus vulgaricus ( Lactobacillus bulgaricus ) and If the mixed strain of Streptococcus thermophilus is less than 1 part by weight relative to 100 parts by weight of the rice eye extract concentrate, or if the temperature is less than 30 ° C, or the fermentation time is less than 15 hours, the fermentation process does not proceed sufficiently. the inherent grain taken can not be removed, the Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus) and If more than Streptococcus Thermo filler's When mixed culture is more than 5 parts by weight against 100 parts by weight of ssalnun extract concentrate or the temperature exceeds 40 ℃ in the fermentation process, or a fermentation time of 25 hours (Streptococcus thermophilus), Odor may occur due to further fermentation processes.
상기 발효 공정으로 인하여 쌀눈 고유의 곡물취를 제거할 수 있다.Due to the fermentation process, it is possible to remove the grain odor inherent in rice eyes.
상기 (c)단계에서 상기 쌀눈추출 유산균 발효액 및 상기 쌀 우유의 혼합비율은 상기 쌀눈추출 유산균 발효액 100중량부 대비 쌀 우유 500~1000중량부일 수 있고, 바람직하게는 600~900중량부일 수 있다.In the step (c), the mixing ratio of the rice eye extract lactic acid bacteria fermentation broth and the rice milk may be 500 to 1000 parts by weight of rice milk, preferably 600 to 900 parts by weight, relative to 100 parts by weight of the rice eye extract lactic acid bacteria fermentation broth.
상기 쌀눈추출 유산균 발효액 및 상기 쌀 우유의 혼합비율이 쌀눈추출 유산균 발효액 100중량부 대비 쌀 우유의 함량이 500중량부 미만인 경우, 쌀눈추출 유산균 발효액의 함량이 너무 높아 경제적이지 못할 수 있고, 쌀눈추출 유산균 발효액 100중량부 대비 쌀 우유의 함량이 1000중량부를 초과하는 경우, 쌀눈추출 유산균 발효액의 함량이 너무 낮아 쌀눈에서 추출한 영양소의 함량이 감소할 수 있다.When the mixing ratio of the rice lance lactic acid bacteria fermentation broth and the rice milk is less than 500 parts by weight of rice milk relative to 100 parts by weight of rice lance lactic acid bacteria fermentation broth, the content of rice lance lactic acid bacteria fermentation broth may be too high and economical, rice lance lactic acid bacteria When the content of rice milk is more than 1000 parts by weight relative to 100 parts by weight of the fermentation broth, the content of the lactobacillus lactic acid bacteria fermentation broth is too low, the content of nutrients extracted from the rice eye may be reduced.
또한, 쌀눈추출 유산균 발효액 및 쌀 우유 혼합 시 온도는 바람직하게 75~85℃이고, 시간은 바람직하게 45~75분이다.In addition, the temperature at the time of mixing the lactic acid bacteria fermentation broth and rice milk is preferably 75 ~ 85 ℃, the time is preferably 45 ~ 75 minutes.
상기 혼합 시 온도가 70℃ 미만이거나, 시간이 30분 미만인 경우, 쌀눈추출 유산균 발효액에 쌀 우유가 충분히 혼합되지 않아 소비자가 상기 쌀눈발효음료 조성물 섭취 시 맛에 대한 만족도가 낮을 수 있고, 온도가 90℃를 초과하거나 시간이 90분을 초과하는 경우, 쌀눈추출 유산균 발효액에 포함되어 있는 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus)의 혼합균주로 인해 추가 발효공정이 진행되어 발효로 인한 냄새가 발생할 수 있다.If the mixing temperature is less than 70 ℃, or less than 30 minutes, rice milk is not sufficiently mixed in the fermentation broth of rice lanceolate fermentation broth may not have enough customer satisfaction when ingesting the fermented beverage composition, and the temperature is 90 if it exceeds ℃ or time exceeds 90 minutes, and Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus) that is included in the lactic acid fermentation liquor and extracted ssalnun Due to the mixed strain of Streptococcus thermophilus, an additional fermentation process may occur, resulting in odor from fermentation.
또한, 상기(c)단계에서 쌀눈추출 유산균 발효액 및 쌀 우유 혼합물에 이소말토올리고당, 액산 과당 또는 결정과당, 구연산, 천연 과즙 및 천연 과일향을 첨가하여 쌀눈발효음료 조성물을 제조할 수 있다.In addition, in the step (c) may be prepared by the addition of isomaltooligosaccharide, lactic acid fructose or crystalline fructose, citric acid, natural fruit juice and natural fruit flavor to the rice lance extract lactic acid bacteria fermentation broth and rice milk mixture.
또한, 상기 쌀눈발효음료 조성물은 쌀눈추출 유산균 발효액 5~15중량%, 쌀 우유 75~85중량%, 이소말토올리고당 1~5중량%, 결정과당 또는 액상과당 2~6중량%, 구연산 0.1~1중량%, 천연 과즙 0.5~1.5중량% 및 천연 과일향 0.015~0.15중량%를 포함할 수 있다.In addition, the fermented beverage composition of rice bran is 5-15% by weight of lactic acid bacteria fermentation broth, 75-85% by weight of rice milk, 1-5% by weight of isomaltooligosaccharide, 2-6% by weight of fructose or liquid fructose, 0.1-1 citric acid It may include weight percent, natural fruit juice 0.5-1.5% by weight and natural fruit flavor 0.015-0.15% by weight.
상기 (c)단계 이후, 상기 쌀눈발효음료 조성물을 180~220bar, 바람직하게는 190~210bar에서 고압질균기에 1~5회, 바람직하게는 1~3회 통과시켜 균질화 시키고, 70~100℃, 바람직하게 80~90℃에서 5~30분, 바람직하게 10~20분간 교반하여 살균시킬 수 있다.After the step (c), homogenizing the rice snow fermented beverage composition by passing 1 to 5 times, preferably 1 to 3 times through the autoclave at 180 to 220 bar, preferably 190 to 210 bar, and preferably 70 to 100 ° C. It can be sterilized by stirring at 80 ~ 90 ℃ 5-30 minutes, preferably 10-20 minutes.
이하, 실시예 및 실험예를 들어 본 발명을 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples.
실시예Example
미강에서 분리한 쌀눈((주)에이원이쌀눈, 쌀눈조아) 1중량부 대비 물 10중량부를 혼합기에 투입하여 90℃에서 4시간 동안 쌀눈 추출물을 추출하였다. 추출 후 굴정당도계 기준으로 당도가 5brix가 될 때까지 60℃, -0.5~-0.6bar에서 진공농축기(경서산업, Cosmos660)를 이용하여 쌀눈추출 농축액을 제조하였다. 상기 쌀눈추출 농축액을 교반기에서 5000rpm으로 10분간 균질한 후, 95℃에서 15분간 살균 처리하였다. 살균 후, 40℃로 방냉한 후, 상기 쌀눈추출 농축액에 MRS broth를 이용하여 배양한 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주를 쌀눈추출 농축액 100중량부 대비 3중량부를 접종하여 37℃에서 18시간 동안 혐기성 발효시켜 쌀눈추출 유산균 발효액을 제조하였다. 발효 공정 후, 상기 쌀눈추출 유산균 발효액 80g, 쌀 우유(서강유업(주), 라이스 밸리) 647.2g, 이소말토올리고당 28g, 결정과당 32g, 구연산 4g, 사과농축액 8g 및 사과향 0.8g을 60℃에서 1시간 동안 교반하여 혼합하여 쌀눈발효음료 조성물을 제조하였다. 혼합 공정 후, 200bar의 압력에서 고압균질기에 2회 통과시켜 균질시킨 후, 85℃에서 15분간 살균하였다. 상기 살균 공정 후, 살균된 쌀눈발효음료 조성물을 10℃까지 냉각하여 쌀눈발효음료 조성물을 제조하였다.10 parts by weight of water was added to a mixer with 1 g of rice snow (Co., Ltd. A-one rice snow, rice snow Joa) separated from rice bran, and the rice snow extract was extracted at 90 ° C. for 4 hours. After extraction, the rice extract extract concentrate was prepared using a vacuum concentrator (Gyeongseo, Cosmos660) at 60 ° C and -0.5 ~ -0.6bar until the sugar content reached 5brix. The rice extract concentrate was homogenized at 5000 rpm for 10 minutes in a stirrer, and then sterilized for 15 minutes at 95 ℃. After sterilization, and then allowed to cool to 40 ℃, the using the MRS broth extract concentrate for ssalnun a Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus) and incubated Streptococcus Thermo filler's (Streptococcus thermophilus) and a mixed culture inoculated with three parts by weight compared to 100 parts by weight of the concentrated solution was extracted ssalnun during anaerobic fermentation at 37 ℃ 18 hours to prepare a ssalnun extracted lactic acid fermentation liquor. After the fermentation process, 80 g of the above-mentioned rice lactic acid bacteria fermentation broth, rice milk (Seogang Dairy Co., Ltd., Rice Valley) 647.2 g, isomaltoligosaccharides 28 g, fructose 32 g, citric acid 4 g, apple concentrate 8 g and apple flavor 0.8 g at 60 ℃ The mixture was stirred for an hour to prepare a fermented beverage composition. After the mixing process, the mixture was passed through a high pressure homogenizer twice at a pressure of 200 bar and homogenized, and then sterilized at 85 ° C. for 15 minutes. After the sterilization process, the sterilized rice fermented beverage composition was cooled to 10 ° C. to prepare a rice fermented beverage composition.
상기 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus)의 혼합균주는 Chr.Hansen(YF-L811, Denmark)에서 구매하였다.The Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus) and Mixed strains of Streptococcus thermophilus It was purchased from Chr. Hansen (YF-L811, Denmark).
비교예 1Comparative Example 1
실시예에서 쌀눈 100중량부 대비 물 100중량부를 사용한 것을 제외하고는 실시예와 동일한 방법으로 쌀눈발효음료 조성물을 제조하였다.Except for using 100 parts by weight of water compared to 100 parts by weight of rice eye in the same manner as in Example was prepared a fermented beverage composition.
비교예 2Comparative Example 2
실시예에서 쌀눈 100중량부 대비 물 2000중량부를 사용한 것을 제외하고는 실시예와 동일한 방법으로 쌀눈발효음료 조성물을 제조하였다.Rice fermented beverage composition was prepared in the same manner as in Example, except that 2000 parts by weight of water relative to 100 parts by weight of rice was used.
비교예Comparative example 3 3
실시예에서 발효 공정 시 온도 25℃ 및 시간 10시간으로 설정하고, 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주를 쌀눈추출 농축액 100중량부 대비 0.5중량부를 접종한 것을 제외하고는 실시예와 동일한 방법으로 쌀눈발효음료 조성물을 제조하였다. Example settings in the fermentation process, the temperature and time of 10 hours, 25 ℃, Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus) and Streptococcus Thermo filler's ssalnun a fermented beverage composition of the mixed culture (Streptococcus thermophilus) in the same manner and is as in Example, except that 100 parts by weight of concentrated liquid extract was inoculated ssalnun than 0.5 parts by weight was prepared.
비교예Comparative example 4 4
실시예에서 발효 공정 시 온도 45℃ 및 시간 30시간으로 설정하고, 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주를 쌀눈추출 농축액 100중량부 대비 6중량부를 접종한 것을 제외하고는 실시예와 동일한 방법으로 쌀눈발효음료 조성물을 제조하였다. In the embodiment in the fermentation process temperature is set to 45 ℃ and time 30 hours, Lactobacillus vulgaricus ( Lactobacillus bulgaricus ) and Streptococcus Thermo filler's ssalnun a fermented beverage composition of the mixed culture (Streptococcus thermophilus) in the same manner and is as in Example 6 except that the parts of the inoculation, based on the weight of 100 parts by weight of ssalnun extract concentrate was prepared.
비교예Comparative example 5 5
실시예에서 쌀눈추출 유산균 발효액 및 쌀 우유 혼합 공정 시 온도 60℃, 시간 15분으로 설정하고, 쌀 우유를 쌀눈추출 유산균 발효액 100중량부 대비 300중량부를 첨가한 것을 제외하고는 실시예와 동일한 방법으로 쌀눈발효음료 조성물을 제조하였다.Except for setting the temperature of the eye extract lactic acid bacteria fermentation broth and rice milk in the embodiment at a temperature of 60 ℃, time 15 minutes, except that 300 parts by weight of rice milk extract lactic acid
비교예Comparative example 6 6
실시예에서 쌀눈추출 유산균 발효액 및 쌀 우유 혼합 공정 시 온도 100℃, 시간 105분으로 설정하고, 쌀 우유를 쌀눈추출 유산균 발효액 100중량부 대비 1200중량부를 첨가한 것을 제외하고는 실시예와 동일한 방법으로 쌀눈발효음료 조성물을 제조하였다.Except for setting the temperature of the rice eye extract lactic acid bacteria fermentation broth and rice milk at the
실험예 1Experimental Example 1
본 발명의 쌀눈발효음료 조성물의 관능성을 확인하기 위해 상기 실시예 및 비교예 1 내지 6에 따라 제조된 쌀눈발효음료 조성물에 대하여 대학생, 대학원생, 주부, 회사원 등의 무작위 인원 30명을 대상으로 향, 맛, 단맛 및 선호도 4가지 항목에 대한 관능평가를 실시하였다. 평점을 1점(매우나쁨)~5점(매우좋음) 사이에서 평가하도록 하는 5점 평점법을 사용하였고, 이에 대한 결과를 하기 표1에 나타내었다.In order to check the functionality of the rice frost fermented beverage composition of the present invention for the rice frost fermented beverage composition prepared according to Examples and Comparative Examples 1 to 6 to 30 random personnel, such as college students, graduate students, housewives, office workers Sensory evaluation was conducted on four items, taste, sweetness and preference. A five-point scoring method was used to evaluate the rating between 1 point (very bad) and 5 points (very good), and the results are shown in Table 1 below.
상기 표 1을 참고하면, 본 발명인 쌀눈발효음료 조성물(실시예)의 경우, 향, 맛, 단맛 및 선호도에 대한 전반적인 평가가 우수함을 알 수 있다.Referring to Table 1, in the case of the present invention rice fermented beverage composition (example), it can be seen that the overall evaluation of the flavor, taste, sweetness and preference is excellent.
이에 대하여, 쌀눈 및 물의 함량비를 1:1로 조절한 경우(비교예 1)는 모든 평가에 있어서, 본 발명(실시예)과 비교했을 때 점수가 낮음을 알 수 있고, 쌀눈 및 물의 함량비를 1:20으로 조절한 경우에는 신맛이 본 발명(실시예)보다 0.2점 높았으나, 다른 항목에서는 본 발명(실시예)과 비교할 때 전체적으로 낮은 점수를 받았음을 알 수 있다.On the other hand, when the ratio of the content of rice and water is adjusted to 1: 1 (Comparative Example 1), in all evaluations, it can be seen that the score is lower compared to the present invention (Example), and the content ratio of rice and water When adjusted to 1:20, the sour taste was 0.2 points higher than the present invention (Example), but it can be seen that the other item received a lower overall score compared to the present invention (Example).
또한, 발효 공정의 온도, 시간 및 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주의 함량을 조절한 경우(비교예 3 및 4)는 본 발명(실시예)과 비교할 때, 향과 단맛에서 매우 낮은 점수를 받았음을 알 수 있고, 이는 발효가 제대로 되지 않았거나(비교예 3), 추가 발효가 진행(비교예 4)되어 발효로 인한 냄새가 발생했음을 알 수 있다.Further, the temperature of the fermentation process, the time and Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus) and When adjusting the content of the mixed culture of Streptococcus Thermo filler's (Streptococcus thermophilus) (Comparative Examples 3 and 4) can know that I am a very low score on, flavor and sweet taste as compared to the present invention (Examples), This can be seen that the fermentation is not properly (Comparative Example 3), or further fermentation proceeds (Comparative Example 4), the odor caused by the fermentation.
또한, 혼합 공정의 온도, 시간 및 쌀 우유의 함량을 조절한 경우(비교예 5 및 6)는 본 발명(실시예)과 비교할 때, 향, 신맛, 단맛 및 외관에서 모두 낮은 점수를 받았음을 알 수 있다.In addition, when the temperature of the mixing process, the time and the content of the rice milk (Comparative Examples 5 and 6) compared to the present invention (Example), it was found that the score was lower in all the flavor, sour, sweet and appearance Can be.
실험예Experimental Example 2 2
본 발명의 쌀눈발효음료 조성물의 항상화 효능을 측정하기 위해 실시예에서 제조된 쌀눈발효음료 조성물(Sample) 및 양성대조군(Ascorbic acid)의 라디칼 소거능 실험을 하기와 같이 진행하였고, 그 결과를 도 1에 나타내었다.In order to measure the homeostatic efficacy of the fermented beverage composition of the present invention, the experiment of radical scavenging ability of the prepared rice eye fermented beverage composition (Sample) and the positive control group (Ascorbic acid) was carried out as follows. Shown in
하기 실험에 이용되는 2,2-diphenyl-1-picrylhydrazyl(DPPH) 및 Ascorbic acid는 Sigma-Aldrich(Korea)에서 구입하여 사용하였다. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ascorbic acid used in the following experiment were purchased from Sigma-Aldrich (Korea).
실험방법Experiment method
1. DPPH를 95(v/v)% ethanol에 녹여서 0.1 mM DPPH 시약을 제조하였으며, 양성 대조군으로는 Ascorbic acid을 0.025~0.5 mg/ml 농도로 제조하여 사용하였다. 1. A 0.1 mM DPPH reagent was prepared by dissolving DPPH in 95 (v / v)% ethanol. As a positive control, Ascorbic acid was prepared at a concentration of 0.025 to 0.5 mg / ml.
2. 실시예(sample) 및 양성 대조군을 각각 200㎕로 제조하여 0.1 mM DPPH 시약 800㎕과 혼합하여 3분 동안 vortexing으로 균일하게 섞어준 뒤 암실에 30분 동안 방치하여 반응시켰다. 2. A sample and a positive control were prepared in 200 μl each, mixed with 800 μl of 0.1 mM DPPH reagent, and then uniformly mixed by vortexing for 3 minutes, and left in the dark for 30 minutes for reaction.
3. DPPH 라디칼 소거능은 517㎚에서 흡광도를 측정하여 나타내었으며, 하기 계산식을 통하여 백분율로 나타내었다.3. DPPH radical scavenging ability was measured by absorbance at 517 nm, and expressed as a percentage through the following formula.
[계산식][formula]
도 1을 참조하면, 실시예(sample)의 경우, 양성 대조군으로 사용한 ascorbic acid에 비해서 낮은 항산화 활성이 확인되었다. Ascorbic acid의 경우 0.1㎎/㎖의 농도에서 95.6%의 높은 라디칼 소거능을 나타냈으며 그 이상의 농도에서는 차이가 없었다. 실시예(sample)의 경우, 농도 의존적으로 라디칼 소거능이 증가하는 것을 확인할 수 있었고, 실시예(sample)의 경우, 0.1㎎/㎖의 농도에서 61.8%의 라디칼 소거능을 나타냈으며, 그 이상의 농도에서는 차이가 없었다.Referring to FIG. 1, in the case of the sample, low antioxidant activity was confirmed as compared to ascorbic acid used as a positive control. Ascorbic acid showed a high radical scavenging activity of 95.6% at the concentration of 0.1 ㎎ / mL, and no difference was observed at higher concentrations. In the case of the sample, it was confirmed that the radical scavenging ability was increased in a concentration-dependent manner, and in the case of the sample, the radical scavenging ability was 61.8% at the concentration of 0.1 mg / ml, and the difference was higher in the concentration above. There was no.
즉, 본 발명에 따라 제조된 실시예(sample)의 경우, 높은 수준의 항산화능이 있음을 알 수 있다.That is, in the case of the sample prepared according to the present invention, it can be seen that there is a high level of antioxidant capacity.
실험예Experimental Example 3 3
본 발명의 쌀눈발효음료 조성물의 항상화 효능을 측정하기 위해 실시예에서 제조된 쌀눈발효음료 조성물(Sample) 및 양성대조군(Ascorbic acid)의 총 폴리페놀의 함량을 하기와 같이 측정하였고, 그 결과를 도 2에 나타내었다.In order to measure the homeostatic efficacy of the fermented beverage composition of the present invention, the total polyphenol content of the fermented beverage composition (Sample) and the positive control group (Ascorbic acid) prepared in Examples was measured as follows. 2 is shown.
하기 실험에 이용되는 Na2CO3, Folin&Ciocalteus phenol reagent 및 Ascorbic acid는 Sigma-Aldrich(Korea)에서 구입하여 사용하였다. Na 2 CO 3 , Folin & Ciocalteus phenol reagent and Ascorbic acid used in the following experiment were purchased from Sigma-Aldrich (Korea).
측정방법How to measure
1. Na2CO3는 증류수에 희석하여 2중량% 농도로 제조하였다.1. Na 2 CO 3 was diluted to distilled water to prepare a concentration of 2% by weight.
2. 양성 대조군으로는 Ascorbic acid을 0.025~2.5㎎/㎖농도로 제조하였다.2. Ascorbic acid was prepared in a concentration of 0.025 ~ 2.5mg / ㎖ as a positive control.
3. 실시예(sample) 및 양성 대조군 100㎕에 2% Na2CO3 2㎖를 혼합하여 3분 동안 방치하였다. 3. 2 ml of 2% Na 2 CO 3 was mixed with 100 µl of the sample and the positive control and left for 3 minutes.
4. 50% Folin&Ciocalteu's phenol reagent 100㎕를 상기 실시예(sampel) 및 양성대조군에 혼합한 뒤 암실에서 30분 동안 방치한 후 750nm에서 흡광도를 측정하였다.4. 100 μl of 50% Folin & Ciocalteu's phenol reagent was mixed in the example (sampel) and the positive control group, and then left for 30 minutes in the dark to measure absorbance at 750 nm.
5. 실시예(sample)에 대한 총 폴리페놀 함량을 확인하기 위해 양성 대조군으로 사용된 ascorbic acid의 농도 범위를 분포하여 독립적으로 제조한 후, calibration curve를 이용하여 측정 범위 내 직선성을 확인하였고, 양성 대조군의 농도는 3회 반복 측정하여 평균치를 데이터로 사용하였다.5. Independently prepared by distributing the concentration range of ascorbic acid used as a positive control in order to confirm the total polyphenol content for the sample (sample), the linearity in the measurement range was confirmed using a calibration curve, The concentration of the positive control was measured three times and the average value was used as data.
도 2를 참조하면, 작성한 양성 대조군 ascorbic acid의 검량선 회귀식은 y= 1.2068x - 0.0324이며, 직선성 (r2) 값은 0.9973으로 높은 직선성을 나타냈고, 실시예(sample)의 총 폴리페놀 함량은 2.26㎎/㎖을 나타냈음을 알 수 있다.Referring to FIG. 2, the calibration curve regression equation of the prepared positive control ascorbic acid was y = 1.2068x-0.0324, and the linearity (r 2 ) value was 0.9973, indicating high linearity, and the total polyphenol content of the sample (sample). It can be seen that represents 2.26 mg / ml.
즉, 본 발명에 따라 제조된 실시예(sample)의 경우, 다량의 폴리페놀을 포함하고 있으므로, 뛰어난 항산화효능이 있음을 알 수 있다.That is, in the case of the sample prepared according to the present invention, since it contains a large amount of polyphenols, it can be seen that there is an excellent antioxidant effect.
이와 같이, 본 발명에 따라 항산화 효능을 갖는 쌀눈추출물을 락토바실러스 불가리쿠스(Lactobacillus bulgaricus ) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus )의 혼합균주로 발효시켜 곡물취를 제거한 쌀눈추출 유산균 발효액에 쌀 우유를 혼합함으로써, 우유의 첨가 없이도, 소비자의 관능성을 만족시키는 쌀눈발효음료 조성물을 제공할 수 있게 된다.As such, rice eye extract having antioxidant efficacy according to the present invention is Lactobacillus vulgaricus ( Lactobacillus bulgaricus ) and Fermented with a mixed strain of Streptococcus thermophilus ( Septococcus thermophilus ) by mixing the rice milk in the lactobacillus fermentation broth from which the grain odor is removed, it is possible to provide a rice eye fermentation beverage composition that satisfies the consumer's sensuality without the addition of milk Will be.
이상으로 본 발명의 제조예, 실험예 및 도면을 참고하여 상세하게 설명하였다. 본 발명의 설명은 예시를 위한 것이며, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자는 본 발명의 기술적 사상이나 필수적인 특징을 변경하지 않고서 다른 구체적인 형태로 쉽게 변형이 가능하다는 것을 이해할 수 있을 것이다.It has been described in detail with reference to the production examples, experimental examples and drawings of the present invention. The description of the present invention is for illustrative purposes, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present invention.
따라서, 본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허청구범위에 의하여 나타내어지며, 특허청구범위의 의미, 범위 및 그 균등 개념으로부터 도출되는 모든 변경 또는 변형된 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.Therefore, the scope of the present invention is shown by the claims below rather than the detailed description, and all changes or modifications derived from the meaning, scope, and equivalent concepts of the claims are included in the scope of the present invention. Should be interpreted.
Claims (3)
(b)상기 쌀눈추출농축액에 식물성 유산균을 접종한 후, 발효조에 첨가하여 30~40℃에서 15~25시간 동안 발효 배양하여 쌀눈추출 유산균 발효액을 제조하는 단계; 및
(c)상기 쌀눈추출 유산균발효액에 쌀 우유를 혼합하여 70~90℃에서 30~90분 동안 교반하여 쌀눈발효음료 조성물을 제조하는 단계;
를 포함하고,
상기 (a)단계는 상기 쌀눈 및 물의 혼합 비율이 상기 쌀눈 100중량부 대비 상기 물 500~1500중량부이고, 상기 (b)단계는 상기 식물성 유산균이 락토바실러스 불가리쿠스(Lactobacillus bulgaricus) 및 스트렙토코커스 써모필러스(Streptococcus thermophilus)의 혼합 균주이고, 상기 쌀눈추출농축액 및 혼합균주의 접종비율은 상기 쌀눈추출농축액 100중량부 대비 상기 혼합균주 1~5중량부이고, 상기 (c)단계는 상기 쌀눈추출 유산균 발효액 및 상기 쌀 우유의 혼합 비율이 상기 쌀눈추출 유산균 발효액 100중량부 대비 상기 쌀 우유 500~1000중량부이고,
항산화 효능이 있는 것을 특징으로 하는 쌀눈발효음료 조성물 제조방법. (a) after mixing the rice snow and water separated from the rice bran to prepare a rice snow extract extracted for 2-6 hours at 80 ~ 100 ℃, and extract the rice snow by vacuum concentration until the rice snow extract to 1 ~ 10brix on the basis of refractive sugar meter Preparing a concentrate;
(b) inoculating the vegetable lactic acid bacteria to the rice extract extract, and then added to the fermenter to fermentation culture for 15 to 25 hours at 30 ~ 40 ℃ to prepare a rice eye extract lactic acid bacteria fermentation broth; And
(c) mixing the rice milk with the lactose extract lactic acid fermentation broth to stir for 30 to 90 minutes at 70 to 90 ° C. to prepare a fermented rice beverage composition;
Including,
The step (a) is the mixing ratio of the rice eye and water is 500 to 1500 parts by weight of water compared to 100 parts by weight of the rice eye, the step (b) is the plant lactic acid bacteria Lactobacillus vulgaricus ( Lactobacillus bulgaricus) and Of Streptococcus thermophilus A mixed strain, the inoculation ratio of the rice extract extract and the mixed strain is 1 to 5 parts by weight of the mixed strain compared to 100 parts by weight of the rice extract extract concentrate, the step (c) is the mixing of the rice eye extract lactic acid bacteria fermentation broth and the rice milk The ratio is 500 to 1000 parts by weight of the rice milk relative to 100 parts by weight of the rice eye extract lactic acid bacteria fermentation broth,
Rice fermented beverage composition manufacturing method characterized by having an antioxidant effect.
상기 (c)단계는 상기 쌀눈추출 유산균발효액 및 상기 쌀 우유 혼합물에 이소말토올리고당, 액산 과당 또는 결정과당, 구연산, 천연 과즙 및 천연 과일향을 혼합하고, 항산화 효능이 있는 것을 특징으로 하는 쌀눈발효음료 조성물 제조방법.
The method of claim 1,
The step (c) is a rice snow fermented beverage, characterized in that the mixture is mixed with isomaltooligosaccharide, liquid acid fructose or fructose, citric acid, natural fruit juice and natural fruit flavor in the fermented lactic acid bacteria fermentation broth and rice milk mixture, and has an antioxidant effect Method for preparing the composition.
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