KR102063646B1 - SNP marker set for discrimination of subspecies in rice and uses thereof - Google Patents

SNP marker set for discrimination of subspecies in rice and uses thereof Download PDF

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KR102063646B1
KR102063646B1 KR1020180131005A KR20180131005A KR102063646B1 KR 102063646 B1 KR102063646 B1 KR 102063646B1 KR 1020180131005 A KR1020180131005 A KR 1020180131005A KR 20180131005 A KR20180131005 A KR 20180131005A KR 102063646 B1 KR102063646 B1 KR 102063646B1
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고희종
서정환
김백기
김탁
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Abstract

본 발명은 벼의 아종 감별용 SNP 마커 세트 및 이의 용도에 관한 것으로, 본 발명의 192개 SNP 마커는 벼의 12개 염색체 전체에 분포하며, 대량으로 신속, 정확하게 벼의 아종을 감별할 수 있으므로, 벼의 품종 및 계통의 아종 구분, 아종간 잡종후대의 형질연관유전자좌 분석 및 품종 육성 등에 다양하게 활용될 수 있을 것이다.The present invention relates to a set of SNP markers for discriminating a subspecies of rice and its use. The 192 SNP markers of the present invention are distributed over all 12 chromosomes of rice, and can discriminate subspecies of rice quickly and accurately in large quantities. Subspecies classification of rice varieties and strains, analysis of transgenic gene loci after hybridization between subspecies, and breeding may be used.

Description

벼의 아종 감별용 SNP 마커 세트 및 이의 용도{SNP marker set for discrimination of subspecies in rice and uses thereof}SNP marker set for discrimination of subspecies in rice and uses approximately}

본 발명은 벼의 아종 감별용 SNP 마커 세트 및 이의 용도에 관한 것이다.The present invention relates to a set of SNP markers for discriminating subspecies of rice and their use.

벼는 세계 4대 식량작물의 하나로, 식물학적으로 벼속(Oryza)에는 야생벼를 포함해 24개의 종이 있다. 이 가운데 재배벼는 전 세계적으로 분포하는 아시아종(Oryza sativa L.)과 서아프리카 일부지역에서 밭벼로 재배하는 아프리카종(Oryza glaberrima Steud.) 두 가지 종뿐이다. 아프리카종은 유전적 변이가 단순한 반면, 아시아종은 매우 다양한 생태형(ecotype)으로 분화하여 각 지역으로 전파, 재배지역이 확대되면서 자연환경에 적응하기 위한 오랜 진화과정을 통해 자연적으로 이뤄졌다.Rice is one of the world's four largest food crops. Botanically, Oryza has 24 species, including wild rice. There are only two types of cultivated rice, the Asian species ( Oryza sativa L.), which are distributed worldwide, and the African species ( Oryza glaberrima Steud.), Which is grown in some parts of West Africa. While African species have a simple genetic variation, Asian species have evolved into a wide variety of ecotypes, spreading and spreading to various regions, and have been naturally developed through long evolutionary processes to adapt to the natural environment.

벼는 품종들간의 잡종불임 정도와 형태적 차이 등에 따라 일명 안남미로 불리는 인디카(indica)와 한국과 일본인이 주로먹는 자포니카(japonica)로 구분되는 두 아종(subspecies)이 있고, 상기 두 아종은 유전체 염기서열에 있어서 차이가 있는 것으로 보고되었다. 또한, 인디카 품종들은 쌀알이 길쭉하고 끈기가 없어 부슬부슬 흩어지는 열대 및 아열대 지역에 적응한 열대형으로서, 전세계 쌀 생산량의 90%를 차지하며 병해충 저항성이 강한 특징이 있고, 온대 자포니카 품종들은 온대에 적응한 형태로서 낟알이 짧고 둥글며 기름지고 찰기가 많으며, 수량성은 다소 떨어지지만 내냉성이 좋고, 식미가 우수한 특징이 있다. 육종학자들은 이러한 인디카와 자포니카의 장점들을 결합시켜 수량성이 높고, 병해충 저항성이 강하며, 내냉성이 강하고 식미가 우수한 이상적인 품종을 육성하고자 노력해왔다. 그러나, 인디카와 자포니카간 교배된 잡종후대에서는 불임이 심하게 발생하고 우량형질들간에 재조합이 잘 일어나지 않아 이들의 육종이 성공적이지 못하였으나, 지금까지 아종간 재조합이 제약되는 원인조차 구명되어 있지 못하고 있다. 따라서 인디카와 자포니카간 재조합이 잘 일어나지 않는 유전학적인 원인 구명과, 재조합 정도를 측정할 수 있는 마커의 개발이 요구되고 있다.Rice has two subspecies, which are classified into indica, which is called Annam South, and japonica, which are mainly eaten by Koreans and Japanese, depending on the degree of hybrid infertility and morphological differences among varieties. It has been reported that there is a difference in sequence. Indica varieties are tropical and subtropical, with grains of rice that are long and sticky and scattered. They account for 90% of the world's rice production and are resistant to pests, and temperate japonica varieties are temperate. Adapted form is short, round, oily and sticky, with low water quality but good cold resistance and good taste. Breeders have tried to combine these indicar and japonica advantages to produce ideal varieties with high yields, pest resistance, cold resistance and good taste. However, in the hybrid progeny hybridized between Indica and Japonica, infertility is severe and recombination does not occur well between superior traits, but their breeding has not been successful, but until now, the cause of restriction between subspecies has not been determined. Therefore, there is a need to identify genetic causes in which recombination between indica and japonica does not occur and to develop markers capable of measuring the degree of recombination.

본 발명자들은 이전의 연구를 통해 벼의 아종 특이적 마커를 보고한 바 있으나, 기존에 보고된 벼의 아종 특이적 마커는 벼 전체 유전체를 포함하지 못하여 실제 벼 육종 및 유전연구 활동에 한계가 있었으며, 겔 기반의 PCR 마커로 대량의 시료와 마커 분석에 시간과 노동력이 많이 소비되는 단점이 있었다. 이에 본 발명에서는, 벼 12개 염색체 전체에서 두 아종인 인디카와 자포니카를 효율적으로 감별할 수 있는 192개의 아종 특이적 SNP(single nucleotide polymorphism) 마커를 선별하였고, 다수의 시료에서 다수의 표적 핵산 서열을 분석할 수 있는 플루다임(Fluidigm) 시스템을 이용하여, 벼의 두 아종을 효과적으로 감별할 수 있음을 확인하였다.The present inventors have reported subtype specific markers of rice through previous studies, but previously reported subtype specific markers of rice did not include the entire genome of rice and had limitations in actual rice breeding and genetic research activities. Gel-based PCR markers have the disadvantage of consuming a lot of time and labor in analyzing a large amount of samples and markers. Therefore, in the present invention, 192 subspecies specific SNP (single nucleotide polymorphism) markers capable of efficiently discriminating two subspecies, Indica and Japonica, were selected from all 12 chromosomes of rice. Using an analytical fluidigm system, it was confirmed that two subspecies of rice could be effectively discriminated.

최근에는 여러 작물에서 NGS(next generation sequencing)를 통해 해독된 유전체 정보를 기반으로 분자마커를 발굴하여 대량의 분자마커를 빠르게 확보하고 있으며, 분자마커는 DNA 서열에서 일어나는 변이의 종류에 따라 나뉘는데, 최근 NGS 기술의 발달과 더불어 이용되는 대표적인 분자마커는 SNP, SSR(simple sequence repeat), InDel(insertion/deletion) 등이 있다. 이 중, SNP는 개체 간의 DNA에 나타나는 하나의 염기 차이로, 매우 다양한 종의 게놈에 걸쳐서 존재한다. 식물체 게놈에 나타나는 빈번한 SNP는 유전자 지도제작(mapping), 분자표지 보조 육종(marker assisted breeding) 및 유전자지도 기반 클로닝을 가능하게 하였다.Recently, a large number of molecular markers are quickly obtained by discovering molecular markers based on genomic information decoded through next generation sequencing (NGS) in various crops, and molecular markers are divided according to types of mutations occurring in DNA sequences. Representative molecular markers used with the development of NGS technology include SNP, simple sequence repeat (SSR), and insertion / deletion (InDel). Of these, SNPs are one base difference that appears in DNA between individuals, and exist across a wide variety of genomes. Frequent SNPs appearing in the plant genome have enabled gene mapping, marker assisted breeding, and genetic map based cloning.

한편, 한국등록특허 제0736152호에는 '벼에서의 아종 감별용 STS 마커 세트'가 개시되어 있고, 한국등록특허 제0844468호에는 '국내 및 일본 자포니카 벼 품종판별용 마이크로새틀라이트 마커 조합 및 이의 코드'가 개시되어 있으나, 본 발명의 벼의 아종 감별용 SNP 마커 세트 및 이의 용도에 대해서는 기재된 바가 없다.Meanwhile, Korean Patent No. 0836152 discloses 'STS marker set for discriminating subspecies in rice', and Korean Patent No. 0844468 describes 'combination of microsatellite markers for discriminating domestic and Japanese japonica rice varieties and codes thereof'. Is disclosed, but there is no description of the SNP marker set for subspecies discrimination of rice of the present invention and its use.

본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명자들은 벼 12개 염색체 전체에서 두 아종(subspecies)인 인디카(indica)와 자포니카(japonica)를 효율적으로 감별할 수 있는 192개의 아종 특이적 SNP(single nucleotide polymorphism) 마커를 선별하였고, 상기 SNP 마커를 확인할 수 있는 플루다임(Fluidigm) 시스템용 프라이머 세트를 제작하여 다양한 지리적 기원의 30개 벼 품종, 및 인디카와 자포니카 품종의 교잡으로 육성된 F2 190개체에 대해 유전자형을 분석한 결과, 모든 시료에 대해서 벼의 두 아종을 효과적으로 감별할 수 있음을 확인함으로써, 본 발명을 완성하였다.The present invention is derived from the above requirements, and the present inventors can effectively distinguish between two subspecies, indica and japonica, from all 12 chromosomes of rice. (single nucleotide polymorphism) were selected for the marker, the 30 rice varieties of different geographical origin to produce a fluorenyl dime (Fluidigm) system primer sets that can determine the SNP marker, and grown by the hybridization of the Indica and japonica varieties F 2 As a result of genotyping of 190 individuals, the present invention was completed by confirming that two subspecies of rice could be effectively discriminated against all samples.

상기 과제를 해결하기 위해, 본 발명은 서열번호 1 내지 768로 표시된 올리고뉴클레오티드에서 인접한 4개의 올리고뉴클레오티드가 하나의 프라이머 세트인 것을 특징으로 하는 192개의 올리고뉴클레오티드 프라이머 세트를 포함하는, 벼의 아종(subspecies) 감별용 프라이머 세트를 제공한다.In order to solve the above problems, the present invention is a subspecies of rice comprising 192 oligonucleotide primer sets, characterized in that four adjacent oligonucleotides in the oligonucleotides represented by SEQ ID NOS: 1 to 768 are one primer set. ) Provides a primer set for differentiation.

또한, 본 발명은 상기 프라이머 세트; 및 증폭 반응을 수행하기 위한 시약을 포함하는 벼의 아종 감별용 키트를 제공한다.In addition, the present invention is a primer set; And it provides a rice seed discriminating kit comprising a reagent for performing the amplification reaction.

또한, 본 발명은 벼 시료에서 게놈 DNA를 분리하는 단계; 상기 분리된 게놈 DNA를 주형으로 하고, 상기 프라이머 세트를 이용하여 증폭 반응을 수행하여 표적 서열을 증폭하는 단계; 및 상기 증폭 단계의 산물의 유전자형을 결정하는 단계;를 포함하는, 벼의 아종을 감별하는 방법을 제공한다.In addition, the present invention comprises the steps of separating genomic DNA from rice samples; Amplifying a target sequence by using the isolated genomic DNA as a template and performing an amplification reaction using the primer set; And determining the genotype of the product of the amplification step. It provides a method for discriminating subspecies of rice.

종래 보고된 벼의 아종 감별 분자마커는, 벼 전체 유전체를 포함하지 못하여 실제 벼 육종 및 유전연구 활용에 한계가 있었다. 그러나, 본 발명의 192개 SNP 마커는 벼의 12개 염색체 전체에 분포하며, 상기 SNP 마커를 이용하여 대량으로 신속, 정확하게 벼의 아종을 감별할 수 있으므로, 벼의 품종 및 계통의 아종 구분, 아종간 잡종후대의 형질연관유전자좌 분석 및 품종 육성 등에 다양하게 활용될 수 있을 것이다.The previously reported molecular markers for discriminating subspecies of rice do not include the entire genome of rice, and thus have practical limitations on rice breeding and genetic research. However, the 192 SNP markers of the present invention are distributed over all 12 chromosomes of rice, and by using the SNP markers, the subspecies of rice varieties and strains can be distinguished quickly and accurately in large quantities. It can be used for analysis of transgene genetic locus and breeding of varieties.

도 1은 벼 12개 염색체 상에서의 192개 SNP 마커의 물리적 위치를 보여주는 도식이다.
도 2는 본 발명의 192개 SNP 마커를 이용한 30개 벼 품종의 유전자형 분석 결과를 UPGMA(Unweighted Pair Group Method with Arithmetic Mean)를 이용하여 분석한 계통수 도식이다.
도 3은 본 발명의 192개 SNP 마커를 이용한 TN1(인디카 품종)과 도봉(자포니카 품종)의 교잡으로 육성된 F2 190개체의 유전자형 분석 결과로, A는 산점도를, B는 call map을 나타낸다. 빨간색: XX allele, 녹색: YY allele, 파란색: XY(hetero) allele.
도 4는 본 발명의 192개 SNP 마커 세트를 이용하여 작성한, TN1과 도봉의 교잡으로 육성된 F2 190개체 집단의 유전연관지도이다.
도 5는 본 발명의 192개 SNP 마커 세트를 이용하여 양친과 BC2F1(Hopum*3/Dular) 두 개체의 유전자형 분석 결과이다.1 is a schematic showing the physical location of 192 SNP markers on 12 chromosomes of rice.
2 is a phylogenetic diagram of genotype analysis results of 30 rice varieties using 192 SNP markers of the present invention using UPGMA (Unweighted Pair Group Method with Arithmetic Mean).
FIG. 3 shows genotyping results of 190 F 2 190 individuals grown by hybridization of TN1 (Indica cultivar) and Dobong (japonica cultivar) using 192 SNP markers of the present invention, where A is a scatter plot and B is a call map. Red: XX allele, Green: YY allele, Blue: XY (hetero) allele.
4 is a genetic association map of a population of F 2 190 individuals grown by hybridization of TN1 and Dobong, prepared using the 192 SNP marker sets of the present invention.
5 shows genotyping results of two parents and two individuals of BC2F1 (Hopum * 3 / Dular) using the 192 SNP marker sets of the present invention.

본 발명의 목적을 달성하기 위하여, 본 발명은 서열번호 1 내지 768로 표시된 올리고뉴클레오티드에서 인접한 4개의 올리고뉴클레오티드가 하나의 프라이머 세트인 것을 특징으로 하는 192개의 올리고뉴클레오티드 프라이머 세트를 포함하는, 벼의 아종(subspecies) 감별용 프라이머 세트를 제공한다.In order to achieve the object of the present invention, the present invention is a subspecies of rice comprising 192 oligonucleotide primer sets, characterized in that four adjacent oligonucleotides in the oligonucleotides represented by SEQ ID NOS: 1-768 are one primer set. (subspecies) Provide a set of primers for differentiation.

본 발명에 따른 프라이머 세트에 있어서, 상기 벼의 아종은 자포니카(Oryza sativa L. subsp. japonica K.) 및 인디카(O. sativa L. subsp. indica K.)인 것을 특징으로 한다.In the primer set according to the present invention, the subspecies of rice are japonica ( Oryza sativa L. subsp . Japonica K.) and indica ( O. sativa L. subsp . Indica K.).

본 발명의 프라이머 세트는 연속되는 서열번호 4개의 올리고뉴클레오티드가 하나의 프라이머 세트를 이루며, 4개의 올리고뉴클레오티드 프라이머는 각각 ASP(SNPtype assay allele specific primer)1, ASP2, LSP(SNPtype assay locus specific primer) 및 STA(SNPtype assay specific target amplification primer) 프라이머이다. STA와 LSP는 목표 염기서열 증폭을 위해 사용되는 프라이머 세트이며, LSP와 ASP는 SNP 위치 염기를 확인하기 위해 사용되는 프라이머 세트이고, ASP1 및 ASP2는 SNP(single nucleotide polymorphism) 위치 염기에서 나타난 다형성에 대해 각각의 대립 형질에 특이적인 올리고뉴클레오티드 프라이머로, ASP 프라이머의 3' 말단 최종 염기가 SNP 위치 염기를 나타낸다(표 2 내지 표 7 참고).In the primer set of the present invention, a sequence of four oligonucleotides consisting of contiguous SEQ ID NOs comprises one primer set, and the four oligonucleotide primers are SNPtype assay allele specific primers (ASP) 1, ASP2, SNPtype assay locus specific primers (LSP), and STA (SNPtype assay specific target amplification primer) primer. STA and LSP are primer sets used for amplification of target sequences, LSP and ASP are primer sets used to identify SNP position bases, and ASP1 and ASP2 are polymorphisms expressed at single nucleotide polymorphism (SNP) position bases. As oligonucleotide primers specific for each allele, the 3 'terminal final base of the ASP primer represents the SNP position base (see Tables 2-7).

본 발명에 따른 192개의 프라이머 세트는 SNP 위치염기의 유전형을 결정할 수 있는 프라이머 세트로, 표 2 내지 표 7에 개시된 각 SNP의 allele에서 앞에 기술된 염기는 자포니카 아종의 염기를, 뒤에 기술된 염기는 인디카 아종의 염기를 나타낸다.192 primer set according to the present invention is a primer set that can determine the genotype of the SNP position base, the base described in the allele of each SNP shown in Table 2 to Table 7 is the base of the japonica subspecies, The base of the indica subspecies is shown.

또한, 본 발명의 상기 프라이머는 서열번호 1 내지 768;의 염기서열의 부가, 결실 또는 치환된 서열도 포함할 수 있다.In addition, the primer of the present invention may include a sequence of addition, deletion or substitution of the nucleotide sequence of SEQ ID NOs: 1 to 768;

본 발명에 있어서, "프라이머"는 카피하려는 핵산 가닥에 상보적인 단일 가닥 올리고뉴클레오티드 서열을 말하며, 프라이머 연장 산물의 합성을 위한 개시점으로서 작용할 수 있다. 상기 프라이머의 길이 및 서열은 연장 산물의 합성을 시작하도록 허용해야 한다. 프라이머의 구체적인 길이 및 서열은 요구되는 DNA 또는 RNA 표적의 복합도(complexity)뿐만 아니라 온도 및 이온 강도와 같은 프라이머 이용 조건에 의존할 것이다.In the present invention, "primer" refers to a single stranded oligonucleotide sequence that is complementary to the nucleic acid strand to be copied and may serve as a starting point for the synthesis of the primer extension product. The length and sequence of the primers should allow to start the synthesis of extension products. The specific length and sequence of the primer will depend on the primer utilization conditions such as temperature and ionic strength as well as the complexity of the DNA or RNA target required.

본 발명에 있어서, 프라이머로서 이용된 올리고뉴클레오티드는 또한 뉴클레오티드 유사체(analogue), 예를들면, 포스포로티오에이트(phosphorothioate), 알킬포스포로티오에이트 또는 펩티드 핵산 (peptide nucleic acid)을 포함할 수 있거나 또는 삽입 물질(intercalating agent)을 포함할 수 있다. 또한, 프라이머는 DNA 합성의 개시점으로 작용하는 프라이머의 기본 성질을 변화시키지 않는 추가의 특징을 혼입할 수 있다. 본 발명의 프라이머 핵산 서열은 필요한 경우, 분광학적, 광화학적, 생화학적, 면역화학적 또는 화학적 수단에 의해 직접적으로 또는 간접적으로 검출 가능한 표지를 포함할 수 있다. 표지의 예로는, 효소(예를 들어, HRP (horse radish peroxidase), 알칼리 포스파타아제), 방사성 동위원소(예를 들어, 32P), 형광성 분자, 화학그룹(예를 들어, 비오틴) 등이 있다. 프라이머의 적합한 길이는 사용하고자하는 프라이머의 특성에 의해 결정하지만, 통상적으로 15 내지 30bp의 길이로 사용한다. 프라이머는 주형의 서열과 정확하게 상보적일 필요는 없지만 주형과 혼성복합체(hybrid-complex)를 형성할 수 있을 정도로 상보적이어야만 한다.In the present invention, oligonucleotides used as primers may also comprise nucleotide analogues such as phosphorothioate, alkylphosphothioate or peptide nucleic acid, or It may comprise an intercalating agent. In addition, the primers may incorporate additional features that do not change the basic properties of the primers that serve as a starting point for DNA synthesis. Primer nucleic acid sequences of the present invention may comprise a label, if necessary, detectable directly or indirectly by spectroscopic, photochemical, biochemical, immunochemical or chemical means. Examples of labels include enzymes (eg horse radish peroxidase (HRP), alkaline phosphatase), radioactive isotopes (eg 32 P), fluorescent molecules, chemical groups (eg biotin), and the like. have. The suitable length of the primer is determined by the characteristics of the primer to be used, but is usually used in the length of 15 to 30bp. The primers need not be exactly complementary to the sequence of the template but must be complementary enough to form a hybrid-complex with the template.

본 발명은 또한, 상기 프라이머 세트; 및 증폭 반응을 수행하기 위한 시약을 포함하는 벼의 아종 감별용 키트를 제공한다. 상기 증폭 반응을 수행하기 위한 시약은 DNA 폴리머라제, dNTPs, 및 버퍼를 포함할 수 있으나, 이에 제한되는 것은 아니다. 상기 dNTPs는 dATP, dCTP, dGTP, dTTP를 포함하며, DNA 폴리머라제는 내열성 DNA 중합효소로서 Taq DNA 폴리머라제, Tth DNA 폴리머라제 등 시판되는 폴리머라제를 이용할 수 있다. 또한, 본 발명의 키트는 최적의 반응 수행 조건을 기재한 사용자 설명서를 추가로 포함할 수 있다. 안내서는 키트 사용법, 예를 들면, 역전사 완충액 및 PCR 완충액 제조 방법, 제시되는 반응 조건 등을 설명하는 인쇄물이다. 안내서는 팜플렛 또는 전단지 형태의 안내 책자, 키트에 부착된 라벨, 및 키트를 포함하는 패키지의 표면상에 설명을 포함한다. 또한, 안내서는 인터넷과 같이 전기 매체를 통해 공개되거나 제공되는 정보를 포함한다.The invention also, the primer set; And it provides a rice seed discriminating kit comprising a reagent for performing the amplification reaction. Reagents for performing the amplification reaction may include, but are not limited to, DNA polymerase, dNTPs, and buffers. The dNTPs include dATP, dCTP, dGTP, dTTP, and DNA polymerase may use commercially available polymerases such as Taq DNA polymerase and Tth DNA polymerase as heat resistant DNA polymerase. In addition, the kit of the present invention may further include a user manual describing the conditions for performing the optimal reaction. The guide is a printed document that explains how to use the kit, such as how to prepare reverse transcription buffer and PCR buffer, and the reaction conditions presented. The instructions include brochures in the form of pamphlets or leaflets, labels affixed to the kit, and instructions on the surface of the package containing the kit. In addition, the guide includes information that is disclosed or provided through electronic media such as the Internet.

본 발명은 또한,The present invention also provides

벼 시료에서 게놈 DNA를 분리하는 단계;Separating genomic DNA from the rice sample;

상기 분리된 게놈 DNA를 주형으로 하고, 본 발명의 프라이머 세트를 이용하여 증폭 반응을 수행하여 표적 서열을 증폭하는 단계; 및Amplifying a target sequence by using the isolated genomic DNA as a template and performing an amplification reaction using the primer set of the present invention; And

상기 증폭 단계의 산물의 유전자형을 결정하는 단계;를 포함하는, 벼의 아종을 감별하는 방법을 제공한다.It provides a method for discriminating the subspecies of rice, comprising; determining the genotype of the product of the amplification step.

본 발명의 방법은 벼 시료에서 게놈 DNA를 분리하는 단계를 포함한다. 상기 게놈 DNA를 분리하는 방법은 당업계에 공지된 방법을 이용할 수 있으며, 예를 들면, CTAB 방법을 이용할수도 있고, Wizard prep 키트(Promega 사)를 이용할 수도 있다. 상기 분리된 게놈 DNA를 주형으로 하고, 본 발명의 일 실시예에 따른 프라이머 세트를 프라이머로 이용하여 증폭 반응을 수행하여 표적 서열을 증폭할 수 있다. 표적 핵산을 증폭하는 방법은 중합효소연쇄반응(polymerase chain reaction; PCR), 리가아제 연쇄반응(ligase chain reaction), 핵산 서열 기재 증폭(nucleic acid sequence-based amplification), 전사 기재 증폭시스템(transcription-based amplification system), 가닥 치환 증폭(strand displacement amplification) 또는 Qβ 복제효소(replicase)를 통한 증폭 또는 당업계에 알려진 핵산 분자를 증폭하기 위한 임의의 기타 적당한 방법이 있다. 이 중에서, PCR이란 중합효소를 이용하여 표적 핵산에 특이적으로 결합하는 프라이머 쌍으로부터 표적 핵산을 증폭하는 방법이다. 이러한 PCR 방법은 당업계에 잘 알려져있으며, 상업적으로 이용가능한 키트를 이용할 수도 있다.The method includes separating genomic DNA from a rice sample. The genomic DNA may be separated by a method known in the art. For example, the CTAB method may be used, or the Wizard prep kit (Promega) may be used. Using the isolated genomic DNA as a template, the target sequence may be amplified by performing an amplification reaction using a primer set according to an embodiment of the present invention as a primer. Methods for amplifying target nucleic acids include polymerase chain reaction (PCR), ligase chain reaction, nucleic acid sequence-based amplification, and transcription-based amplification systems. Amplification systems, strand displacement amplification or amplification via Qβ replicase or any other suitable method for amplifying nucleic acid molecules known in the art. Among these, PCR is a method of amplifying a target nucleic acid from a pair of primers specifically binding to the target nucleic acid using a polymerase. Such PCR methods are well known in the art, and commercially available kits may be used.

상기 증폭 단계 산물의 유전자형을 결정하는 방법은 당업계에 알려진 다양한 방법에 의하여 이루어질 수 있다. 예를 들면, DNA 칩, 모세관 전기영동 등을 통해 수행될 수 있으나, 이에 제한되지 않는다. 모세관 전기영동은 예를 들면, ABI Sequencer를 이용할 수 있다. 또한, 디데옥시법에 의한 직접적인 핵산의 뉴클레오티드 서열의 결정을 통하여 이루어지거나, SNP(single nucleotide polymorphism) 부위의 서열을 포함하는 프로브 또는 그에 상보적인 프로브를 상기 DNA와 혼성화시키고 그로부터 얻어지는 혼성화 정도를 측정함으로써 다형성 부위의 뉴클레오티드 서열을 결정하는 방법 등이 이용될 수 있으나, 이에 제한되지는 않는다.
The method of determining the genotype of the amplification step product may be made by various methods known in the art. For example, it may be performed through DNA chips, capillary electrophoresis, and the like, but is not limited thereto. Capillary electrophoresis can use, for example, an ABI Sequencer. In addition, by determining the nucleotide sequence of the nucleic acid directly by the dideoxy method, or by hybridizing a probe comprising a sequence of a single nucleotide polymorphism (SNP) site or a probe complementary thereto with the DNA and measuring the degree of hybridization obtained therefrom. A method of determining the nucleotide sequence of the polymorphic site may be used, but is not limited thereto.

이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.
Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

실시예 1. 각 96개 아종특이적 SNP(single nucleotide polymorphism)로 구성된 마커 세트의 개발Example 1 Development of a Marker Set Composed of 96 Subspecies-Specific Single Nucleotide Polymorphisms (SNPs)

Rice Diversity SNP 데이터(http://www.ricediversity.org/data), HapRice world SNP 데이터(http://qtaro.abr.affrc.go.jp/qsnp/snp) 및 서울대학교 작물분자육종연구실에서 보유한 벼 16품종의 리시퀀싱(resequencing) 데이터에서 SNP 정보를 수집하였다. 수집한 SNP 중 기능유전자에 있거나, 밀접히 연관된 14개 SNP를 포함하여, 벼의 12개 염색체 상에서 2~3Mb 또는 약 10cM 간격으로 인디카와 자포니카 사이에서 다형성을 보이는 SNP 192개를 선발하였다. 상기 192개의 벼 아종 특이적 SNP 마커의 벼 염색체 상에서의 분포는 하기 표 1 및 도 1과 같다.Rice Diversity SNP data (http://www.ricediversity.org/data), HapRice world SNP data (http://qtaro.abr.affrc.go.jp/qsnp/snp) and Seoul National University Crop Molecular Breeding Laboratory SNP information was collected from resequencing data of 16 rice varieties. Among the SNPs collected, 192 SNPs were selected that showed polymorphism between indica and japonica at intervals of 2 to 3 Mb or about 10 cM on 12 chromosomes of rice, including 14 SNPs that are functionally or closely related. Distribution of the 192 rice subspecies specific SNP markers on rice chromosomes is shown in Table 1 and FIG. 1.

Figure 112018107378740-pat00001
Figure 112018107378740-pat00001

Fluidigm D3 Assay Design 도구(https://d3.fluidigm.com)를 이용하여 선발된 192개 SNP의 Fluidigm SNP genotyping assay를 개발하였다. 192개 SNP를 기반으로 한 Fluidigm SNP genotyping assay는 2개의 96-plex 인디카/자포니카 SNP 세트로 나누어 제작하였다. 상기 각 assay는 하나의 SNP 마커에 대해 ASP1(allele specific primer 1), ASP2, LSP(locus specific primer) 및 STA(specific target amplification)의 총 4개의 올리고뉴클레오티드 프라이머가 한 세트로 구성된 프라이머 세트 96개를 포함하도록 제작하였다. 벼의 아종 특이적 Fluidigm SNP genotyping assay의 각 세트 정보는 하기 표 2 내지 표 7과 같다.A Fluidigm SNP genotyping assay of 192 SNPs was developed using the Fluidigm D3 Assay Design tool (https://d3.fluidigm.com). The Fluidigm SNP genotyping assay, based on 192 SNPs, was divided into two 96-plex indica / japonica SNP sets. Each assay was performed on 96 primer sets consisting of a set of four oligonucleotide primers, a total of four oligonucleotide primers (ASP1), ASP2, locus specific primer (LSP), and specific target amplification (STA) for one SNP marker. Made to include. Each set of the subspecies specific Fluidigm SNP genotyping assay of rice is shown in Table 2 to Table 7 below.

Figure 112018107378740-pat00002
Figure 112018107378740-pat00002

Figure 112018107378740-pat00003
Figure 112018107378740-pat00003

Figure 112018107378740-pat00004
Figure 112018107378740-pat00004

Figure 112018107378740-pat00005
Figure 112018107378740-pat00005

Figure 112018107378740-pat00006
Figure 112018107378740-pat00006

Figure 112018107378740-pat00007
Figure 112018107378740-pat00007

실시예 2. 벼의 아종 특이적 SNP 마커 세트를 이용한 유전자형 분석Example 2. Genotyping Using Subspecies Specific SNP Marker Set of Rice

아종 특이적 SNP 마커 세트를 이용하여 다양한 지리적 기원지의 30개 벼 품종에 대한 유전자형을 분석하였다. 본 발명에 사용된 벼 품종의 정보는 하기 표 8과 같다.Genotypes were analyzed for 30 rice varieties of various geographical origins using subspecies specific SNP marker sets. Information of rice varieties used in the present invention is shown in Table 8.

Figure 112018107378740-pat00008
Figure 112018107378740-pat00008

유전자형 분석 결과를 토대로 벼 품종 간 유전적 거리를 분석한 결과 30개 벼 품종은 인디카와 자포니카 두 그룹으로 구별되었을 뿐만 아니라, 본 발명의 아종 특이적 SNP 마커는 30개 벼 품종 모두를 특이적으로 구별 가능한 것이 확인되었다(도 2).As a result of analyzing the genetic distance between rice varieties based on genotyping results, not only 30 rice varieties were distinguished into two groups, Indica and Japonica, but the subspecies specific SNP marker of the present invention specifically distinguished all 30 rice varieties. It was confirmed that it was possible (FIG. 2).

또한, 아종 특이적 SNP 마커 세트를 이용하여 인디카 아종인 TN1 품종과 자포니카 아종인 도봉 품종의 교잡으로 육성된 F2 190개체의 유전자형을 분석한 결과, 모부본인 인디카 및 자포니카의 대립 형질뿐만 아니라 헤테로(hetero)의 대립 형질도 정확히 구별할 수 있음이 관찰되었다(도 3).
In addition, genotypes of 190 F 2 190 individuals grown by hybridization between subtidal TN1 strains and Japonica subspecies Dobong cultivars using subspecies specific SNP marker sets showed that heterozygous as well as alleles of parental indica and japonica were identified. It was observed that alleles of hetero) can be accurately distinguished (FIG. 3).

실시예 3. 벼의 아종 특이적 SNP 마커 세트를 이용한 유전자형 분석Example 3. Genotyping Using Rice Subspecies Specific SNP Marker Set

상기 실시예 2의 F2 190개체의 유전자형 결과를 이용하여 F2 190개체 집단의 12개 염색체의 유전연관지도를 QTL IciMapping 4.1을 이용하여 작성하였다(도 4). 이 연관지도는 벼 12개 염색체 전체를 포함하여 농업형질 QTL 분석 및 이상 분리(segregation distortion) 분석 등에 활용될 수 있다.Genetic correlation maps of 12 chromosomes of the F 2 190 population using the genotyping results of 190 F 2 190 individuals of Example 2 were prepared using QTL IciMapping 4.1 (FIG. 4). This map can be used for agricultural quality QTL analysis and segregation distortion analysis, including all 12 chromosomes of rice.

두 개의 목표 유전자에 대해 foreground selection을 진행한 Dular(인디카, 공여친)와 Hopum(자포니카, 반복친)을 여교배 한 BC2F1 두 개체(HP12068-12 및 HP12068-17)의 유전적 배경(background)을 본 발명의 아종특이적 SNP 마커 세트로 분석한 결과, BC2F1 개체의 호품(Hopum) background 회복 정도를 확인할 수 있었다(도 5).Genetic backgrounds of two BC2F1 individuals (HP12068-12 and HP12068-17) that were cross-crossed with Dular (indica, donor) and Hopum (japonica, repeat) who performed foreground selection on two target genes As a result of analyzing the subspecies specific SNP marker set of the present invention, it was able to confirm the degree of recovery of Hop background of BC2F1 individuals (FIG. 5).

<110> Seoul National University R&DB Foundation <120> SNP marker set for discrimination of subspecies in rice and uses thereof <130> PN18361 <160> 768 <170> KoPatentIn 3.0 <210> 1 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 1 gacatttgca agcaacttat actgaca 27 <210> 2 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 2 acatttgcaa gcaacttata ctgacc 26 <210> 3 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 3 tgcttcagag atggcctcga a 21 <210> 4 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 4 gatgatacag gtacaaatga catttgc 27 <210> 5 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 5 gaaatacgcc ggccaaca 18 <210> 6 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 6 gaaatacgcc ggccaacg 18 <210> 7 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 7 tgagaggaag cttggaaagg gag 23 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 8 ttctgttacc atgagtagga gagg 24 <210> 9 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 9 cgtgttaaaa atagagggca ttgaatcaa 29 <210> 10 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 10 cgtgttaaaa atagagggca ttgaatcag 29 <210> 11 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 11 gccctactta ttctatacac caggct 26 <210> 12 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 12 ggtttcccac ttacataaac gtgttaa 27 <210> 13 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 13 gtgctcatct ttagattgat ttgtccat 28 <210> 14 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 14 gtgctcatct ttagattgat ttgtccag 28 <210> 15 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 15 gatggcactt cacattggcc t 21 <210> 16 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 16 gaaccatctt tgaggtgctc at 22 <210> 17 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 17 tctcaattta acaacagcat ctgaagc 27 <210> 18 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 18 gatctcaatt taacaacagc atctgaaga 29 <210> 19 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 19 ccgttgcttt gttggtcgga 20 <210> 20 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 20 aggaaatggg atttctaaat gatctcaa 28 <210> 21 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 21 aatagcatct aaaaacatgt tttacaccga a 31 <210> 22 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 22 agcatctaaa aacatgtttt acaccgag 28 <210> 23 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 23 cggagggatg aaaatttaat ttttctctcc aa 32 <210> 24 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 24 catgaatatg ctaataggaa caatagcatc t 31 <210> 25 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 25 caggctggac attttgactg ac 22 <210> 26 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 26 tcaggctgga cattttgact gat 23 <210> 27 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 27 gtcaagaatc tctgtaagaa atagacaata tgtcagta 38 <210> 28 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 28 gagtcagttt atatggtttc aggct 25 <210> 29 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 29 cgctagtgta gagcacgaac atg 23 <210> 30 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 30 cgctagtgta gagcacgaac atc 23 <210> 31 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 31 ggaccacggt gagccaga 18 <210> 32 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 32 gagatttaca acaagctatg caacg 25 <210> 33 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 33 agggacagat gctgctttgt taa 23 <210> 34 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 34 gggacagatg ctgctttgtt ag 22 <210> 35 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 35 ccgcaacagt aaccagcaca a 21 <210> 36 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 36 tgcgtacttt acgatgaagg ga 22 <210> 37 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 37 gcttcaggat ttcagtagga tccac 25 <210> 38 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 38 gcttcaggat ttcagtagga tccag 25 <210> 39 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 39 cccgctgaag aacgtgctg 19 <210> 40 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 40 tcactagtca tcactaaatc agagctt 27 <210> 41 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 41 gttgatggtc cttgctaaaa gtgg 24 <210> 42 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 42 agttgatggt ccttgctaaa agtga 25 <210> 43 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 43 atcatcacct tcagatttca aacaaaaaga ca 32 <210> 44 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 44 agcacctaat atgaaaagtt gatggtc 27 <210> 45 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 45 agcaatcctg agggccataa c 21 <210> 46 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 46 agcaatcctg agggccataa t 21 <210> 47 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 47 cgtctcatgg caaaggctga 20 <210> 48 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 48 gtcagatatc ctagcagcct ca 22 <210> 49 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 49 ctggtgctgg tttaagcagt aa 22 <210> 50 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 50 ctggtgctgg tttaagcagt ag 22 <210> 51 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 51 ctagaatcat cgtaattact actcatatat gtcaccattt 40 <210> 52 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 52 tcattcttaa ttttactgct ggtgct 26 <210> 53 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 53 aggtggctca cttgaactgt at 22 <210> 54 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 54 aggtggctca cttgaactgt ag 22 <210> 55 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 55 ggaggttcgg ccaaacgttc 20 <210> 56 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 56 gcctatatag aagcttcgat cgatca 26 <210> 57 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 57 ttctgaaact tagcagccag gtaa 24 <210> 58 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 58 tctgaaactt agcagccagg tag 23 <210> 59 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 59 gccccaatac atagctgctg g 21 <210> 60 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 60 gcacaggaag cttgacgttc 20 <210> 61 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 61 acaccgataa acacgttatg acacat 26 <210> 62 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 62 caccgataaa cacgttatga cacac 25 <210> 63 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 63 cccagcagta gcagatccag a 21 <210> 64 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 64 gcataattag catactatga caccga 26 <210> 65 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 65 gatcgatcac ctcgctgct 19 <210> 66 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 66 atcgatcacc tcgctgcc 18 <210> 67 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 67 cgctggtcgc tgccg 15 <210> 68 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 68 cggaggaaaa cacagtagga tc 22 <210> 69 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 69 cagacaatct tcaggcaaag ctc 23 <210> 70 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 70 cagacaatct tcaggcaaag ctt 23 <210> 71 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 71 agtctgaaga tgccgagggt c 21 <210> 72 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 72 atccgaatca gatgaaagat tgca 24 <210> 73 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 73 agtagaaaat gtattcacag ttccagct 28 <210> 74 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 74 agtagaaaat gtattcacag ttccagca 28 <210> 75 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 75 aaaaagttct ggccgtcgca a 21 <210> 76 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 76 gcgtgtaacg ttttatgcag tagaa 25 <210> 77 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 77 tgatacgatt acaggacgtg tactact 27 <210> 78 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 78 tgatacgatt acaggacgtg tactaca 27 <210> 79 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 79 actacaaacc taactaatcg gagatgaaaa tcg 33 <210> 80 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 80 tgatctggac aataatagtc atgatacga 29 <210> 81 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 81 ccgtgtactc gcatgtgaca at 22 <210> 82 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 82 cgtgtactcg catgtgacaa c 21 <210> 83 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 83 gggtgtcagt ttgcccttgc 20 <210> 84 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 84 ttgaggcatg ttcttgccg 19 <210> 85 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 85 tgcctttaat ggagcatcat gga 23 <210> 86 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 86 gcctttaatg gagcatcatg gg 22 <210> 87 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 87 ggattgcatg accatcaagg ca 22 <210> 88 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 88 cccttgctca atttgctgtt g 21 <210> 89 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 89 gttgtttgac tgtacccaaa tgtact 26 <210> 90 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 90 gttgtttgac tgtacccaaa tgtacc 26 <210> 91 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 91 agcaacatct atagttgtcc agggatttt 29 <210> 92 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 92 aaaggtggtg ctatgttgtt gt 22 <210> 93 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 93 gatccgggtg gtgtcagtt 19 <210> 94 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 94 gatccgggtg gtgtcagtc 19 <210> 95 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 95 cagagtaatg ggaaggcatg ca 22 <210> 96 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 96 gagattttac ttgagaaagc agatctctt 29 <210> 97 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 97 cctgttctac cgtcaagcga c 21 <210> 98 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 98 cctgttctac cgtcaagcga t 21 <210> 99 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 99 tccgtgatga tgctaatttt tatttatttt tactaattgt 40 <210> 100 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 100 gatgtacacc atccaatttt acctgt 26 <210> 101 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 101 gaagaagatg ccaagtttgc tgtt 24 <210> 102 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 102 aagaagatgc caagtttgct gtc 23 <210> 103 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 103 agggaagctt cagccatcgt 20 <210> 104 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 104 gacttgcctg aatcctcaga a 21 <210> 105 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 105 tcaaagattc ggatggttcc ca 22 <210> 106 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 106 tcaaagattc ggatggttcc cg 22 <210> 107 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 107 gctgaagctg atccattgca ca 22 <210> 108 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 108 gctgagttgt tcaactcaag ct 22 <210> 109 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 109 ttgccactgt gtgcaacatt t 21 <210> 110 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 110 ttgccactgt gtgcaacatt c 21 <210> 111 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 111 catcggcaac gagacgaaac a 21 <210> 112 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 112 gcatgtgtga atacatggcg t 21 <210> 113 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 113 tcaatctgca catacgggat aaatgt 26 <210> 114 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 114 caatctgcac atacgggata aatgc 25 <210> 115 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 115 ggcgatatat tctcttcatt catgctaaag tg 32 <210> 116 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 116 ctgaggagag atatatcctc tgtcaat 27 <210> 117 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 117 agttaaacaa gtagttactc taaccgttga a 31 <210> 118 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 118 agttaaacaa gtagttactc taaccgttga t 31 <210> 119 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 119 actagctaga aagaaatctc tgactttttg tgt 33 <210> 120 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 120 ggctccatgc atatctatag ttaaacaa 28 <210> 121 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 121 cattccgtgt gaaccacgtt tg 22 <210> 122 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 122 cattccgtgt gaaccacgtt tc 22 <210> 123 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 123 gtgctccaaa tttgccttgg tga 23 <210> 124 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 124 cctccattac ctatgacaaa cacattc 27 <210> 125 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 125 ctcctcgcag aacggctac 19 <210> 126 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 126 ctcctcgcag aacggctaa 19 <210> 127 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 127 cgtccaaggc gctcttgtc 19 <210> 128 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 128 gacggctccg acgatga 17 <210> 129 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 129 ctgaaataga aagggctgtt ttggt 25 <210> 130 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 130 tgaaatagaa agggctgttt tggc 24 <210> 131 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 131 aacataactc tagcaaaaga attggtaggg ta 32 <210> 132 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 132 ggaacatgta tggagtatag tactgaaat 29 <210> 133 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 133 catgccccta attttgcaga act 23 <210> 134 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 134 catgccccta attttgcaga aca 23 <210> 135 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 135 caactgctga gaatttaaat cccatcca 28 <210> 136 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 136 gttagggtgt gcaccaaaca 20 <210> 137 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 137 catgcgttgg tcattgccta c 21 <210> 138 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 138 catgcgttgg tcattgccta a 21 <210> 139 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 139 agtatggcaa accgggagca 20 <210> 140 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 140 catcacattt ttacggggtt atacca 26 <210> 141 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 141 gcttgctggt ttgtcaagtg c 21 <210> 142 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 142 gcttgctggt ttgtcaagtg t 21 <210> 143 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 143 agtttcttta tcttcgcatc ggatgga 27 <210> 144 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 144 ggattgttgt ggacaatttc aactg 25 <210> 145 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 145 ttcaggtact gatgccaagt acc 23 <210> 146 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 146 gattcaggta ctgatgccaa gtact 25 <210> 147 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 147 tgtggactag ccctacgtac tct 23 <210> 148 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 148 tctaaatctg atggtccctg attca 25 <210> 149 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 149 agcagcctcc tcttctgtg 19 <210> 150 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 150 cagcagcctc ctcttctgtt 20 <210> 151 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 151 atgcgaagct tgggcgtg 18 <210> 152 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 152 cccgcctgct ccaaatac 18 <210> 153 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 153 ttccttttca ctgcattaca aatcagac 28 <210> 154 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 154 cttccttttc actgcattac aaatcagat 29 <210> 155 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 155 ggaatatcat catattatag gttgactagc tactcc 36 <210> 156 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 156 ctttcgatcc agtacttcct tttca 25 <210> 157 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 157 accaaactat tgttgtatag gagtaagagc 30 <210> 158 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 158 caccaaacta ttgttgtata ggagtaagag t 31 <210> 159 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 159 cggccagggt tctgtcaaa 19 <210> 160 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 160 cagatcaaca aagtgacacc aaacta 26 <210> 161 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 161 cgtgcaagac catttgaagc tc 22 <210> 162 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 162 cgtgcaagac catttgaagc ta 22 <210> 163 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 163 gggcgccaac accaaaacc 19 <210> 164 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 164 gacaacagcc atgatggacc 20 <210> 165 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 165 gcctgaaaat gcttgtttct ctgt 24 <210> 166 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 166 gcctgaaaat gcttgtttct ctga 24 <210> 167 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 167 tgttcattaa ccgatcggat tccgaat 27 <210> 168 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 168 caaaggtaca tacatataca tctctagcc 29 <210> 169 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 169 tggcaaaggt aaggcctcac 20 <210> 170 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 170 tggcaaaggt aaggcctcaa 20 <210> 171 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 171 cagtgggcaa tcgagcgt 18 <210> 172 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 172 agctcaggga ggtggc 16 <210> 173 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 173 gaactgtccc tttcttggtc atc 23 <210> 174 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 174 ggaactgtcc ctttcttggt catt 24 <210> 175 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 175 gacgcgtgct tgacagcat 19 <210> 176 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 176 aaggcgttga tcagaatgga ac 22 <210> 177 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 177 gccaaacttt gtcagaaaca ggtt 24 <210> 178 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 178 gccaaacttt gtcagaaaca ggtc 24 <210> 179 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 179 gcatgatact tctatcatgt acactcctga 30 <210> 180 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 180 cttcagtact gttagtaacg ttgcc 25 <210> 181 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 181 gcaagccgat tgttacgaac tt 22 <210> 182 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 182 gcaagccgat tgttacgaac tc 22 <210> 183 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 183 gctttgctat gtggttggtg ataca 25 <210> 184 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 184 cgcaaccttc gttagcagtg 20 <210> 185 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 185 agtttcatag atcaaccagt tcgagt 26 <210> 186 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 186 gtttcataga tcaaccagtt cgagc 25 <210> 187 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 187 agatactcga agcgtctgtg ttctttt 27 <210> 188 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 188 ttgccactaa cttgaagcag ttt 23 <210> 189 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 189 cgtaataatg atataataac cgcgctgaat tac 33 <210> 190 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 190 cgtaataatg atataataac cgcgctgaat taa 33 <210> 191 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 191 gctgttgtga tgcgtgggat 20 <210> 192 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 192 gtactccggc ttacgtaata atgat 25 <210> 193 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 193 aaaaaaccta cctgatcaga tccact 26 <210> 194 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 194 aaaaacctac ctgatcagat ccaca 25 <210> 195 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 195 gagatgatat tttatagtgt agtcttacca tcaatttctt 40 <210> 196 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 196 aacctatctc acacacaaaa aacct 25 <210> 197 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 197 acaatccatg gtcggttgat gaa 23 <210> 198 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 198 acaatccatg gtcggttgat gat 23 <210> 199 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 199 gcctgcatga ccatcggc 18 <210> 200 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 200 cccaggacga ctgcac 16 <210> 201 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 201 aacagaagct acaacttcgt gagat 25 <210> 202 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 202 acagaagcta caacttcgtg agac 24 <210> 203 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 203 catctaaaag aaatgcattg ttgctttggc 30 <210> 204 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 204 gcctgatagg tagaggacaa aaca 24 <210> 205 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 205 gtcaaatttc ggcctgttaa ccc 23 <210> 206 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 206 gtcaaatttc ggcctgttaa cct 23 <210> 207 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 207 ccccacaaat acttagcagg cc 22 <210> 208 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 208 ctccaaaaac ctgaaaattt gagtgt 26 <210> 209 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 209 ctcaggaagg ttatcgttgc cta 23 <210> 210 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 210 tcaggaaggt tatcgttgcc tg 22 <210> 211 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 211 caccaactat tcagaatatc gcaagaattt tga 33 <210> 212 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 212 gccgaacaaa atcattcttc aaaaact 27 <210> 213 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 213 caaaatccct tatattattg ctattaaacc tttgat 36 <210> 214 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 214 caaaatccct tatattattg ctattaaacc tttgac 36 <210> 215 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 215 aattacatta ctgtatattt tatattccta tgttgtcaaa 40 <210> 216 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 216 gcaagcaaaa actcaaaatc ccttatat 28 <210> 217 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 217 gccacacata cacatcagca taca 24 <210> 218 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 218 ccacacatac acatcagcat acg 23 <210> 219 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 219 aaaaattcaa atagaaatat atatatcctg tgagcaaaca 40 <210> 220 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 220 acaacaataa cataactgac ctgcc 25 <210> 221 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 221 ggttggtttg gcgatctcat ct 22 <210> 222 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 222 gttggtttgg cgatctcatc a 21 <210> 223 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 223 aggtgctcca gtgctgtaag g 21 <210> 224 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 224 ccatggcttg ttacaagtta cagtg 25 <210> 225 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 225 actgatgtga agaacagtct agagc 25 <210> 226 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 226 cactgatgtg aagaacagtc tagagt 26 <210> 227 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 227 tgcttcatgc catcgatcag ga 22 <210> 228 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 228 gtaggcaaac tgtagtgtca ctg 23 <210> 229 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 229 cggaatctca ttttcttaag ctgcata 27 <210> 230 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 230 cggaatctca ttttcttaag ctgcatt 27 <210> 231 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 231 gtaagaaaat atattggtct attagcctat acaatcctgt 40 <210> 232 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 232 tcatcaattc aacacggaat ctcatt 26 <210> 233 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 233 acgtggctca agctcactg 19 <210> 234 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 234 aacgtggctc aagctcacta 20 <210> 235 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 235 tgcgcaaatc gcagtgtgac 20 <210> 236 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 236 cttgtggagg tggtgca 17 <210> 237 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 237 cataaatcag aattacaggt tgcagct 27 <210> 238 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 238 cataaatcag aattacaggt tgcagca 27 <210> 239 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 239 aaaccttgga ggaatgtcat cgtct 25 <210> 240 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 240 ttcacagttt cacacaggac ataaa 25 <210> 241 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 241 aggcaaaatt ggcagccaaa 20 <210> 242 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 242 aggcaaaatt ggcagccaac 20 <210> 243 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 243 agtgctcatt gggaattttg taattctttg tt 32 <210> 244 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 244 catacctaaa aaggcaaaat tggca 25 <210> 245 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 245 atcagcttga ggtagcttca tctc 24 <210> 246 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 246 tcagcttgag gtagcttcat ctg 23 <210> 247 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 247 gcaggcaaag atgattctta tttattagac cc 32 <210> 248 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 248 tgatatgcag aatgtgtgcc atc 23 <210> 249 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 249 ccaacgggtc gctggtg 17 <210> 250 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 250 ccaacgggtc gctggta 17 <210> 251 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 251 acaggagttc atcatagatt tgcctgt 27 <210> 252 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 252 agaccttgtg tttagcgatt tagc 24 <210> 253 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 253 tcaaggtggt cctcgacact a 21 <210> 254 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 254 caaggtggtc ctcgacactc 20 <210> 255 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 255 gtattgcgct atgccacggt 20 <210> 256 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 256 ctcaagaaag ccaccgacat tt 22 <210> 257 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 257 ggttatcgct tcagcattga atgag 25 <210> 258 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 258 ggttatcgct tcagcattga atgaa 25 <210> 259 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 259 gtgtcttgga caaccatgat ttctgg 26 <210> 260 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 260 ctgagtcata ttccagaaca tggtt 25 <210> 261 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 261 accattttac gtgacccgag aa 22 <210> 262 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 262 accattttac gtgacccgag ag 22 <210> 263 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 263 ctgtcagtgc gtggtgtgt 19 <210> 264 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 264 cagtttatag acggagggag tacc 24 <210> 265 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 265 ctcattggtg ataatgcact tgtatgaat 29 <210> 266 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 266 tcattggtga taatgcactt gtatgaac 28 <210> 267 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 267 gcattctcac aaagtgtata taatgccaga tc 32 <210> 268 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 268 gatgccagta tgtcactcat tgg 23 <210> 269 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 269 tcgttacagc atgtcagtag cc 22 <210> 270 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 270 ctcgttacag catgtcagta gca 23 <210> 271 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 271 gtgcacttcg ttccaaggct 20 <210> 272 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 272 cactgtgctg acgagttaaa atct 24 <210> 273 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 273 caaggcaagt acacttttct ccaat 25 <210> 274 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 274 caaggcaagt acacttttct ccaac 25 <210> 275 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 275 gaccacatgt aatcttagtt tacatgataa aatcgg 36 <210> 276 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 276 caagttgctg caccttttac aag 23 <210> 277 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 277 tgctcctcaa tacatttgat aacagct 27 <210> 278 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 278 gctcctcaat acatttgata acagcc 26 <210> 279 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 279 gaacgcgagt tgtctgcctt 20 <210> 280 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 280 gatatttctc atcaagtttg tgctcc 26 <210> 281 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 281 tgcattgctc cgattgatag gag 23 <210> 282 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 282 gcattgctcc gattgatagg ac 22 <210> 283 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 283 tctattctgg actacagtcc gaacatatca 30 <210> 284 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 284 gcattagaaa gtttcactat atttgtgca 29 <210> 285 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 285 aagatggtga aagccacctg a 21 <210> 286 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 286 agatggtgaa agccacctgg 20 <210> 287 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 287 agggcaacca aaagcattgg tc 22 <210> 288 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 288 cagaaaatta ggttgggacc ctaa 24 <210> 289 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 289 accacatgac ctgatctttg ca 22 <210> 290 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 290 ccacatgacc tgatctttgc g 21 <210> 291 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 291 cagctagaag aatttttgat gcaactcca 29 <210> 292 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 292 gcatatccag atatcattga ggacc 25 <210> 293 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 293 atagaagttc tgaatctgct aacactga 28 <210> 294 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 294 gaagttctga atctgctaac actgc 25 <210> 295 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 295 ttgaaatcgc cggttaccgc 20 <210> 296 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 296 cacagcaccg agcatagaag 20 <210> 297 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 297 gaacaagtcg ctgcatttgg tatta 25 <210> 298 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 298 acaagtcgct gcatttggta ttg 23 <210> 299 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 299 gcgtcaccat tggccct 17 <210> 300 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 300 tggtggccta gtacatgaac a 21 <210> 301 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 301 gctgtcacac tcgaagtgct t 21 <210> 302 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 302 gctgtcacac tcgaagtgct c 21 <210> 303 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 303 gctgacaacg aggtcggtga 20 <210> 304 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 304 ccgacctcgc tgtcac 16 <210> 305 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 305 ccaacttatc caggccatcc a 21 <210> 306 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 306 ccaacttatc caggccatcc c 21 <210> 307 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 307 ttccgcttag ccctctgctt 20 <210> 308 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 308 tggcactgtt gatttgggc 19 <210> 309 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 309 aaaccataaa gctgcttgac agattaaat 29 <210> 310 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 310 aaccataaag ctgcttgaca gattaaag 28 <210> 311 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 311 ggttacctta cattcatgca acatggaa 28 <210> 312 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 312 agcccacctc tcttaaacca ta 22 <210> 313 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 313 gaatgagtca gtcaggcagt gta 23 <210> 314 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 314 tgagtcagtc aggcagtgtg 20 <210> 315 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 315 gcaaactaaa ctaaaaaata tgtctgacag tctca 35 <210> 316 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 316 aagttggatt aacacctgaa atgaaga 27 <210> 317 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 317 agcggctatg ctatgtccg 19 <210> 318 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 318 gagcggctat gctatgtcca 20 <210> 319 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 319 ttttcttatt aaaattacag agaaccttat agcttcagtc 40 <210> 320 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 320 gctagagatg aaaacaaaat gtgagc 26 <210> 321 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 321 tctaggtgca aatggtgttg tca 23 <210> 322 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 322 ctaggtgcaa atggtgttgt cc 22 <210> 323 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 323 ggaagcatgg cactcttgaa agt 23 <210> 324 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 324 acactgatgg atctaggtgc aa 22 <210> 325 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 325 cttctcaaat cgctcaaatg atcct 25 <210> 326 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 326 cttctcaaat cgctcaaatg atcca 25 <210> 327 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 327 tccatggcca agatggggt 19 <210> 328 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 328 ggctttggca ttggcttc 18 <210> 329 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 329 atttgataaa atcgtcatcc aaaccgt 27 <210> 330 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 330 ttgataaaat cgtcatccaa accgc 25 <210> 331 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 331 ggccaacaca agatccccag 20 <210> 332 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 332 ccttatgtag atctagtaaa cactttacat ttga 34 <210> 333 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 333 gtaatcgaca ataatctcaa gttgccg 27 <210> 334 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 334 gtaatcgaca ataatctcaa gttgcca 27 <210> 335 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 335 gactgttcaa cttcagcagg ca 22 <210> 336 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 336 cagtcctgtg tcgtcgtaat c 21 <210> 337 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 337 caattttctg tgagtctttg cgga 24 <210> 338 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 338 aattttctgt gagtctttgc ggg 23 <210> 339 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 339 aagcctgagg gtgcgtaca 19 <210> 340 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 340 aaaatacaat tttctgtgag tctttgcg 28 <210> 341 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 341 gcgcgcgtgt aattttgaaa c 21 <210> 342 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 342 tgcgcgcgtg taattttgaa at 22 <210> 343 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 343 tttgacccct actctaacat gttgct 26 <210> 344 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 344 caatgctggc ctttcttttg tat 23 <210> 345 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 345 tttctgagtc ttgccctgtg t 21 <210> 346 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 346 tctgagtctt gccctgtgc 19 <210> 347 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 347 gatccttgtg ctctccagcc t 21 <210> 348 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 348 ccttggtata ccttgatctt cctttttatt tat 33 <210> 349 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 349 tgcagtagaa agatacaccg cc 22 <210> 350 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 350 atgcagtaga aagatacacc gca 23 <210> 351 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 351 gatcagatgt ggagtttttg tcacttcaa 29 <210> 352 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 352 aagactgtga agaggtcagg at 22 <210> 353 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 353 ttggcccatc gcagaagat 19 <210> 354 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 354 tggcccatcg cagaagac 18 <210> 355 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 355 tgacctctgg agtttcagtg ctc 23 <210> 356 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 356 agggcaacat ccctggg 17 <210> 357 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 357 ctcgtcacag cattagcata tactct 26 <210> 358 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 358 tcgtcacagc attagcatat actcc 25 <210> 359 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 359 aggctatgcg cctatgcca 19 <210> 360 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 360 gctttgtgaa cactaatcac tcgt 24 <210> 361 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 361 acacaccatg ctctaatggc tc 22 <210> 362 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 362 acacaccatg ctctaatggc tt 22 <210> 363 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 363 aggatcgtgg ctcagcatgt 20 <210> 364 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 364 acccaataag gcttttccaa tgaa 24 <210> 365 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 365 agagcttctt ggctccaaga at 22 <210> 366 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 366 agagcttctt ggctccaaga ac 22 <210> 367 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 367 ggccagagct acctgacct 19 <210> 368 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 368 catcccgatc ctctggca 18 <210> 369 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 369 ggagttgtgg gcttaacttt tctc 24 <210> 370 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 370 ggagttgtgg gcttaacttt tctt 24 <210> 371 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 371 ccggcctcaa gatgctcca 19 <210> 372 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 372 aggagttgtg ggcttaactt ttc 23 <210> 373 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 373 gcatcgacag agatccgaaa tgac 24 <210> 374 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 374 gcatcgacag agatccgaaa tgat 24 <210> 375 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 375 gcgacagatg aaatactgga caagc 25 <210> 376 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 376 cacattgctg ctactttttt gca 23 <210> 377 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 377 tcgtcttaaa atataatggt gcacaccta 29 <210> 378 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 378 cgtcttaaaa tataatggtg cacacctc 28 <210> 379 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 379 aaaatattat caacaatatt attttcttaa acacgcatga 40 <210> 380 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 380 tcctttttaa ctctcgtctc gtctta 26 <210> 381 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 381 ggggccactt gttttcaatc a 21 <210> 382 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 382 ggggccactt gttttcaatc c 21 <210> 383 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 383 gggaagtgtt ccctccaggt 20 <210> 384 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 384 actatggatg gcgtgcatat ga 22 <210> 385 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 385 ttatagggca tgtacacacg ct 22 <210> 386 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 386 gggcatgtac acacgcg 17 <210> 387 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 387 gctattctat aaaccttatc aggatttatg gtgaatct 38 <210> 388 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 388 gagcaactga tttgtattgt ctatttttgt 30 <210> 389 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 389 gcatatatta tttcatagga tagtccgcat ct 32 <210> 390 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 390 gcatatatta tttcatagga tagtccgcat cc 32 <210> 391 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 391 aatatgatat gaatacgaat atggtatccc taaaattcaa 40 <210> 392 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 392 gcatatatta tttcatagga tagtccgca 29 <210> 393 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 393 ctcaagtgat cttagtgcca tccta 25 <210> 394 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 394 tcaagtgatc ttagtgccat cctc 24 <210> 395 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 395 actcaatata tgcaccaatt agttctcaca tct 33 <210> 396 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 396 tcttacatgg ccaagatcat actca 25 <210> 397 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 397 cgatgccaat actagccaat gtca 24 <210> 398 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 398 gatgccaata ctagccaatg tcg 23 <210> 399 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 399 tggacatata accgattgct caaaacct 28 <210> 400 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 400 atgccgatac cagccga 17 <210> 401 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 401 cggtgtccag gtaaccgg 18 <210> 402 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 402 cggtgtccag gtaaccga 18 <210> 403 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 403 ggtcacaatc agccgtgcc 19 <210> 404 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 404 tgatcaccaa agtgttgatg cc 22 <210> 405 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 405 ttgatgctaa aaacgccagt gac 23 <210> 406 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 406 gttgatgcta aaaacgccag tgat 24 <210> 407 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 407 tgtgggtagg catcataaaa aggaact 27 <210> 408 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 408 gtactttcgt ttgatactct tcagagtt 28 <210> 409 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 409 tccacggtgg gcttgac 17 <210> 410 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 410 ttccacggtg ggcttgat 18 <210> 411 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 411 acgcccatgg acttcgagt 19 <210> 412 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 412 gcatgatgct cagcatccc 19 <210> 413 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 413 aggtgcttct gatgttagct acg 23 <210> 414 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 414 caggtgcttc tgatgttagc tact 24 <210> 415 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 415 ccaggctgtt taggagttgg c 21 <210> 416 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 416 gaccgtccat tgatgtctca g 21 <210> 417 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 417 gcatttggtg gtacgtgtat catga 25 <210> 418 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 418 catttggtgg tacgtgtatc atgc 24 <210> 419 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 419 agggttcggc gatgatggt 19 <210> 420 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 420 ccatctcgtc caaagatcct tag 23 <210> 421 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 421 catgccaagc caagatatgg g 21 <210> 422 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 422 ccatgccaag ccaagatatg ga 22 <210> 423 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 423 ccaacgagga ggagaacgca 20 <210> 424 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 424 accagactcg gtcatgcc 18 <210> 425 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 425 gcattttcga gaccagacca gg 22 <210> 426 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 426 gcattttcga gaccagacca gt 22 <210> 427 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 427 gcctaggggg cctgaaagta 20 <210> 428 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 428 acatcatgtg caaactagaa ttaagca 27 <210> 429 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 429 gggccaagga gtacacca 18 <210> 430 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 430 gggccaagga gtacacct 18 <210> 431 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 431 tctgctcggc aggcact 17 <210> 432 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 432 atcctgctct acgccgtt 18 <210> 433 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 433 gcttagctag tactacagct aatttgacat 30 <210> 434 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 434 gcttagctag tactacagct aatttgacac 30 <210> 435 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 435 tttgtttatt tagtttttta ttttgttgag gaatgaatga 40 <210> 436 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 436 ggaacaacac ctagcttagc tagt 24 <210> 437 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 437 agcttgtaga tcagctggca aat 23 <210> 438 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 438 gcttgtagat cagctggcaa aa 22 <210> 439 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 439 tgctcgtgca gtagcaggat 20 <210> 440 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 440 ctacatgaaa gtttacatgc aagtagc 27 <210> 441 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 441 cgaagatctt ccacccccg 19 <210> 442 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 442 cgaagatctt ccaccccca 19 <210> 443 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 443 acatttgtat ggtaatttga gatccgattg ttttt 35 <210> 444 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 444 ccaaccaata aggaacatct ttttcc 26 <210> 445 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 445 caacttagtg caacttttaa acatatcgaa aaa 33 <210> 446 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 446 caacttagtg caacttttaa acatatcgaa aac 33 <210> 447 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 447 gcaagcaacc aatgatctaa gataatgaag c 31 <210> 448 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 448 caactggcca acttagtgca 20 <210> 449 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 449 ggcttagcta cttcatcgtt tctagt 26 <210> 450 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 450 gcttagctac ttcatcgttt ctagc 25 <210> 451 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 451 atgcctcttt tagcagtcga ggaa 24 <210> 452 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 452 tccatttggc ttcaaggctt ag 22 <210> 453 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 453 gagtgctgtc ataatattgc actgg 25 <210> 454 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 454 gagtgctgtc ataatattgc actgc 25 <210> 455 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 455 acaacgagca attttatcat gaaaccaaca 30 <210> 456 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 456 cagcagatta atgctattcc agagt 25 <210> 457 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 457 cttgaccgca aaaccggata t 21 <210> 458 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 458 cttgaccgca aaaccggata c 21 <210> 459 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 459 ctcgtttgcc cggttttggt a 21 <210> 460 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 460 ccagtccgat tttcgtccc 19 <210> 461 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 461 ccactcaaag aaggaatcag cg 22 <210> 462 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 462 gccactcaaa gaaggaatca gca 23 <210> 463 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 463 tcaagccccg gcctcc 16 <210> 464 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 464 cgacaggagc agtgcc 16 <210> 465 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 465 gttacagcat aaaggaaagg aaaaccc 27 <210> 466 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 466 gttacagcat aaaggaaagg aaaacct 27 <210> 467 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 467 tccggctcac tgggtatgg 19 <210> 468 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 468 agcaaagtaa atggataatg ttacagca 28 <210> 469 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 469 tccacacatg tcttgtttaa aaccc 25 <210> 470 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 470 gtccacacat gtcttgttta aaacca 26 <210> 471 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 471 cctccttttt ctttctatct gtgagcatct 30 <210> 472 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 472 acatgggtgt tcgtccaca 19 <210> 473 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 473 ttgatcaaag cacacagaaa gcg 23 <210> 474 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 474 cttgatcaaa gcacacagaa agca 24 <210> 475 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 475 tgtggcgtcg atactttaca gttga 25 <210> 476 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 476 caggtgggct tgtgctt 17 <210> 477 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 477 tctgaaaata tgataagacc gggcg 25 <210> 478 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 478 tctgaaaata tgataagacc gggca 25 <210> 479 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 479 gccggttgca ttgcattctc t 21 <210> 480 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 480 ctgactttta ccctattatc gtctctg 27 <210> 481 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 481 gctctgctct ctctgtctcg a 21 <210> 482 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 482 ctctgctctc tctgtctcgg 20 <210> 483 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 483 gggacacgga cagaaaggaa aaaa 24 <210> 484 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 484 gtagcatgaa gtagtgctct gc 22 <210> 485 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 485 ctgcctccag atgctgc 17 <210> 486 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 486 gctgcctcca gatgctga 18 <210> 487 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 487 acagcaggct ggcttactct 20 <210> 488 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 488 aaattcaatc gaagggatcc acg 23 <210> 489 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 489 ggaaggctct gtgcttacgt tta 23 <210> 490 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 490 gaaggctctg tgcttacgtt tc 22 <210> 491 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 491 ccacaagcct gaatacaagg ca 22 <210> 492 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 492 tgttggagga aggctctgt 19 <210> 493 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 493 gatggcgagt ttgacacaga tcta 24 <210> 494 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 494 tggcgagttt gacacagatc tg 22 <210> 495 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 495 tgggaggtcg tcgtcgc 17 <210> 496 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 496 tggtatctgc tctatgctcg at 22 <210> 497 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 497 ccggtccaat tatccgacgt 20 <210> 498 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 498 ccggtccaat tatccgacgc 20 <210> 499 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 499 ccggggaggc taagttgct 19 <210> 500 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 500 gctcctcctg gacatcctc 19 <210> 501 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 501 tgtggaggtc caaacattgt gt 22 <210> 502 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 502 tgtggaggtc caaacattgt ga 22 <210> 503 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 503 ctaggagtct tagaatgttg atctctgaca 30 <210> 504 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 504 cagaatcttt gtggaggtcc aa 22 <210> 505 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 505 cttacaactc actctctcat gctct 25 <210> 506 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 506 ttacaactca ctctctcatg ctcc 24 <210> 507 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 507 gccatgcaaa agatttttat atagattgtg gc 32 <210> 508 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 508 tcgatgatta aaagcgaaag cttaca 26 <210> 509 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 509 tctgaagatc acgagaggaa ggt 23 <210> 510 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 510 ctgaagatca cgagaggaag gc 22 <210> 511 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 511 gttcacatgg gcctccgc 18 <210> 512 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 512 ctaaacgaga gtatgaagaa gctga 25 <210> 513 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 513 actgcacaac aatcaagatc agc 23 <210> 514 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 514 cactgcacaa caatcaagat caga 24 <210> 515 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 515 ctgactgaaa attgtgcatt gtactcact 29 <210> 516 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 516 aaccagaaag tggtgtaaca ctg 23 <210> 517 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 517 tttcgtcgtc tctatcgcca c 21 <210> 518 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 518 ctttcgtcgt ctctatcgcc at 22 <210> 519 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 519 tgacgatgat cagtgcggat ga 22 <210> 520 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 520 accgcagctt tgccac 16 <210> 521 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 521 aggtggtgtt cttttttaat taagacagat tc 32 <210> 522 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 522 aggtggtgtt cttttttaat taagacagat tt 32 <210> 523 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 523 accatttgac agtttgaaaa ggatggtaat aaaaa 35 <210> 524 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 524 gcagaacata aggtggtgtt ctt 23 <210> 525 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 525 tggagttgaa agttgaaact catcaac 27 <210> 526 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 526 gtggagttga aagttgaaac tcatcaat 28 <210> 527 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 527 ggaggtcagt caaatttcta taatctttag aagca 35 <210> 528 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 528 tggagttgaa agttgaaact catca 25 <210> 529 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 529 tcagtgtggc tggaagaagt c 21 <210> 530 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 530 catcagtgtg gctggaagaa gta 23 <210> 531 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 531 gcaagtcacc tctttgttgt tttaacga 28 <210> 532 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 532 tgcagtgtct tcctcagca 19 <210> 533 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 533 gctggatcta tagcccccc 19 <210> 534 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 534 tgctggatct atagccccct 20 <210> 535 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 535 aaatccaaca cgccggaagc 20 <210> 536 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 536 gcttacgcgt ccttttcgt 19 <210> 537 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 537 aaagccacca gcaattgtca ttt 23 <210> 538 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 538 aagccaccag caattgtcat tc 22 <210> 539 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 539 acgtcggaca acaaattgaa tgaaaatatg at 32 <210> 540 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 540 agcaagacac ataaaattga gcctaa 26 <210> 541 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 541 tctgtatctc actgtagtcc tggt 24 <210> 542 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 542 tctgtatctc actgtagtcc tggg 24 <210> 543 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 543 ggatctcaca gtagcctatc aaatgga 27 <210> 544 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 544 ctggagcatt gctgcttct 19 <210> 545 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 545 caaaacggcg gcagaattgt 20 <210> 546 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 546 aaacggcggc agaattgc 18 <210> 547 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 547 gacaaagcta ggattattag atatgatcac caagt 35 <210> 548 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 548 aatgtggcag ctgacacac 19 <210> 549 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 549 aaagctccta tgcaccccta at 22 <210> 550 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 550 aaagctccta tgcaccccta ag 22 <210> 551 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 551 ccatctgctt gatagactaa ttgggca 27 <210> 552 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 552 gctggtacat gagtaccatc ca 22 <210> 553 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 553 catctggcaa cttctgttct atactagta 29 <210> 554 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 554 catctggcaa cttctgttct atactagtt 29 <210> 555 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 555 cagctcgatc gaggatgata gaaca 25 <210> 556 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 556 gttcccttct tcatctggca a 21 <210> 557 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 557 gcgtgccaat attcctgtag tg 22 <210> 558 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 558 atgcgtgcca atattcctgt agta 24 <210> 559 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 559 tgcggaacgc agcctaacta 20 <210> 560 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 560 attatttttt gaacatgcgt gccaa 25 <210> 561 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 561 ggatacatgt gtgcgagtgc a 21 <210> 562 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 562 gatacatgtg tgcgagtgcg 20 <210> 563 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 563 gggaagaaca gggccacg 18 <210> 564 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 564 gtcaaccgat gaggttgtac ttt 23 <210> 565 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 565 gtatatgatt cccctgacca ggaataag 28 <210> 566 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 566 gtatatgatt cccctgacca ggaataat 28 <210> 567 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 567 ggcattagca gcatctttcc tttgt 25 <210> 568 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 568 cgagccatgc agcagtatat g 21 <210> 569 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 569 cagccgtcca aacggttc 18 <210> 570 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 570 cagccgtcca aacggttt 18 <210> 571 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 571 gtacagcaaa ctcttctata tatgatctgt tcgt 34 <210> 572 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 572 acgccgtcct gatcatcg 18 <210> 573 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 573 tgtacgttgt ccaaatacat ctctagaaa 29 <210> 574 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 574 atgtacgttg tccaaataca tctctagaat 30 <210> 575 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 575 gcatgtcctg aagtactact cctgt 25 <210> 576 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 576 gaggatgtat atctttgaca tgtacgtt 28 <210> 577 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 577 gggagggaaa ggactccc 18 <210> 578 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 578 agggagggaa aggactcca 19 <210> 579 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 579 agtattaaac aaacagaaga aaggataatg atactctgt 39 <210> 580 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 580 cgctgctgct gcatcaaa 18 <210> 581 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 581 gcttcttttg ttaatgatga ttagttcata cagta 35 <210> 582 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 582 gcttcttttg ttaatgatga ttagttcata cagtc 35 <210> 583 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 583 cacctttctt tcttctttct tttctctgaa tgt 33 <210> 584 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 584 gtgcttcttt tgttaatgat gattagttca 30 <210> 585 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 585 tcatcaggaa gaacatctgc aagt 24 <210> 586 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 586 catcaggaag aacatctgca agg 23 <210> 587 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 587 tcgatctgaa taagagggga aacaaagaat 30 <210> 588 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 588 tgcttgtgtt gttctgttgt tca 23 <210> 589 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 589 ccgaattagg cataaatttg gcacg 25 <210> 590 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 590 ccgaattagg cataaatttg gcacc 25 <210> 591 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 591 tgctgcacag attgcacgac 20 <210> 592 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 592 tgatcatcag ctaattccac cga 23 <210> 593 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 593 tgaggaataa tcgacgatca gcaaa 25 <210> 594 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 594 tgaggaataa tcgacgatca gcaag 25 <210> 595 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 595 ccaggggaaa caattcggct t 21 <210> 596 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 596 cgcaagctat aagatatcat aggatgag 28 <210> 597 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 597 ctgatactaa gggaaacaaa atgcatgaa 29 <210> 598 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 598 tgatactaag ggaaacaaaa tgcatgac 28 <210> 599 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 599 ctcctgggca ttggcatgaa 20 <210> 600 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 600 ctccagaggt aatttttgga ccat 24 <210> 601 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 601 tgaagcatgg ccttgaagac t 21 <210> 602 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 602 gaagcatggc cttgaagacc 20 <210> 603 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 603 acagtgtgtg gagatcaagg acat 24 <210> 604 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 604 cggtgatgta ccagtgtaaa tgaaa 25 <210> 605 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 605 aaattgtgaa ggacaaaaac atccgtta 28 <210> 606 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 606 ttgtgaagga caaaaacatc cgttt 25 <210> 607 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 607 agtgctgtga tgagtagcaa tcattga 27 <210> 608 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 608 ccaagtgaaa aatggtaaga aattgtga 28 <210> 609 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 609 cttactatgc tcccaaatta agcgtc 26 <210> 610 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 610 tcttactatg ctcccaaatt aagcgtt 27 <210> 611 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 611 accagggcac gcaggt 16 <210> 612 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 612 acaagagtaa tactccactg ttcttact 28 <210> 613 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 613 tccacacgga cacggtttc 19 <210> 614 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 614 tccacacgga cacggtttt 19 <210> 615 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 615 acaaaacaat aatggaatgg atctgaaaag tgt 33 <210> 616 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 616 acacactcaa agagaagatc tatggt 26 <210> 617 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 617 tgaacttatt ccttcacatt attgccaac 29 <210> 618 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 618 gtgaacttat tccttcacat tattgccaat 30 <210> 619 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 619 agcacgagca cgagaactca 20 <210> 620 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 620 agggacctag agtgaactta ttcc 24 <210> 621 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 621 caatgctgga gtccatatca aaatcaa 27 <210> 622 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 622 caatgctgga gtccatatca aaatcat 27 <210> 623 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 623 cttgttcctt gatattttag atcttaatta actttgtgta 40 <210> 624 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 624 cccgatcgta atgcaatgct 20 <210> 625 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 625 acctgtactc ctgtaggttt aggtt 25 <210> 626 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 626 tacctgtact cctgtaggtt taggta 26 <210> 627 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 627 cgtcttgcca agcaagcca 19 <210> 628 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 628 ctagctagct actgtacatg tacct 25 <210> 629 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 629 gattcattta gctcagctta gctgtg 26 <210> 630 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 630 gattcattta gctcagctta gctgtc 26 <210> 631 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 631 cccacactag ctgactgtcc t 21 <210> 632 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 632 cggcctccat cttaatcgat tc 22 <210> 633 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 633 ccccaaacta ttacagttgc tcg 23 <210> 634 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 634 accccaaact attacagttg ctca 24 <210> 635 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 635 gggtgttaaa tatctagtat ttgagtttaa ggaggta 37 <210> 636 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 636 gggaaaaaag aagtttgaat tacccc 26 <210> 637 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 637 aattaatgtt tgtcctcacc tatttcgttt ag 32 <210> 638 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 638 aattaatgtt tgtcctcacc tatttcgttt aa 32 <210> 639 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 639 tttacgtggt tttttcaaag aaaataataa taatgaagga 40 <210> 640 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 640 atgtttgtcc tcacctattt cgttt 25 <210> 641 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 641 tgctctttct tctgctttga ctttg 25 <210> 642 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 642 gtgctctttc ttctgctttg actttt 26 <210> 643 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 643 gcgaatggac acggcagc 18 <210> 644 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 644 cgtcaatgca ttgcagtgct 20 <210> 645 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 645 caaagccttc tcaagggaag aac 23 <210> 646 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 646 caaagccttc tcaagggaag aag 23 <210> 647 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 647 gcttcttgga ctcttcgaga cct 23 <210> 648 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 648 tcctggagga agaacaagaa ca 22 <210> 649 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 649 actgacaaat acctgccaca ca 22 <210> 650 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 650 ctgacaaata cctgccacac g 21 <210> 651 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 651 tcaaactcca acttttattt tgaacactgc t 31 <210> 652 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 652 cataccgtta acatgcaaaa caact 25 <210> 653 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 653 cagataacag ggacatagtg gacata 26 <210> 654 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 654 agataacagg gacatagtgg acatg 25 <210> 655 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 655 gagccgccta agattttcct ttcg 24 <210> 656 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 656 ggccgaccta tggatatata cagat 25 <210> 657 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 657 gcagtgggat ggcagc 16 <210> 658 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 658 tgcagtggga tggcagt 17 <210> 659 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 659 cggcgctttg ctgcagg 17 <210> 660 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 660 tggtggtatc tcatcgtatt tttgaag 27 <210> 661 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 661 ctatataaaa aaagaggggc gatcctaaac 30 <210> 662 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 662 tctatataaa aaaagagggg cgatcctaaa a 31 <210> 663 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 663 tctttcttaa gataattcat agcataatag gaggaggt 38 <210> 664 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 664 ctatataaaa aaagaggggc gatcctaa 28 <210> 665 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 665 atctctagta aaagggtgcc atacg 25 <210> 666 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 666 gatctctagt aaaagggtgc cataca 26 <210> 667 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 667 agcctatagc cataatgatg tcattgtga 29 <210> 668 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 668 catactacag ccatggaaat gatct 25 <210> 669 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 669 ccacaaagac actgacacca att 23 <210> 670 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 670 ccacaaagac actgacacca atc 23 <210> 671 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 671 ctgcccccgg ccaatg 16 <210> 672 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 672 gtgtcaggac aggtgaatcc 20 <210> 673 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 673 tcttccacca aacttgcatg aattc 25 <210> 674 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 674 tcttccacca aacttgcatg aattt 25 <210> 675 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 675 agaaaatacc atcctcaagg agcaca 26 <210> 676 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 676 actccaaatt tgttgcacct ctt 23 <210> 677 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 677 ctcaaggccg tacatccata tgta 24 <210> 678 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 678 tcaaggccgt acatccatat gtg 23 <210> 679 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 679 ccttctctaa tattcacgtc ttttagattt acgct 35 <210> 680 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 680 gatagttgct tactcttctc aaggc 25 <210> 681 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 681 atctaaaggc gagcaaagag gt 22 <210> 682 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 682 ctaaaggcga gcaaagaggc 20 <210> 683 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 683 tcctttagct cggcaactcg t 21 <210> 684 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 684 gatgatagta ataaatctaa aggcgagca 29 <210> 685 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 685 tgagcccgag cttgacataa 20 <210> 686 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 686 tgagcccgag cttgacatac 20 <210> 687 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 687 gctgaggctc aaagctaggg t 21 <210> 688 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 688 ccgatcccct ctcgtgt 17 <210> 689 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 689 atggatcaac aaaattgtgc tcctattt 28 <210> 690 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 690 gatggatcaa caaaattgtg ctcctatta 29 <210> 691 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 691 caaagtgagc aagggtcagc a 21 <210> 692 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 692 ctaaacacca gggatatgat ggatc 25 <210> 693 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 693 gcccaagcac actcactaat tttta 25 <210> 694 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 694 gcccaagcac actcactaat ttttt 25 <210> 695 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 695 tactagtatt aaactatagg ggattagtgt agatcatttt 40 <210> 696 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 696 ggctggagta aaatctgccc 20 <210> 697 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 697 ctactcctac gtgttctgtt ccaat 25 <210> 698 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 698 actcctacgt gttctgttcc aac 23 <210> 699 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 699 gctctctgaa atagtatacg tgtaaagctt agt 33 <210> 700 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 700 ctttctctgt gttgtgtgct act 23 <210> 701 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 701 agaaattccc tttcaccttt tgcg 24 <210> 702 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 702 aagaaattcc ctttcacctt ttgca 25 <210> 703 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 703 agcgtgagga tggatccaac a 21 <210> 704 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 704 caatacgtct tgatcaggaa ctgac 25 <210> 705 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 705 aactgcaatt ctaggagtgg ct 22 <210> 706 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 706 aactgcaatt ctaggagtgg ca 22 <210> 707 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 707 cgaggctatg ttatgttcta tgttcatgac t 31 <210> 708 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 708 gaacatgggc ataatctcat atagaca 27 <210> 709 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 709 ctatatatta ttattaattg cttacacctc gttttc 36 <210> 710 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 710 ctatatatta ttattaattg cttacacctc gttttt 36 <210> 711 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 711 gtggtcaaat taaagtcaca tttaaaatac catacca 37 <210> 712 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 712 ctcaccagct tgctgtacta tatattatta tt 32 <210> 713 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 713 cgaaagagag ggactactat ttatttatga tgtc 34 <210> 714 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 714 cgaaagagag ggactactat ttatttatga tgta 34 <210> 715 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 715 tggacggagc tagcattgtc at 22 <210> 716 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 716 catcctctaa tacgaaagag aggga 25 <210> 717 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 717 tgagggcaat gcatttgtga tacta 25 <210> 718 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 718 agggcaatgc atttgtgata ctg 23 <210> 719 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 719 ccatcaataa tgcatggagt catgatgag 29 <210> 720 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 720 gctaacacct gattggttag ctt 23 <210> 721 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 721 gttgcgtgaa gacctcagaa attta 25 <210> 722 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 722 ttgcgtgaag acctcagaaa tttg 24 <210> 723 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 723 gttgttttct tcccggtata aatcactgt 29 <210> 724 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 724 acaaccttga ggtgttgcg 19 <210> 725 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 725 aatttagatt tcatccagaa ttgagttgca 30 <210> 726 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 726 aaatttagat ttcatccaga attgagttgc t 31 <210> 727 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 727 ctggattgat tgatatatag atatgtttga ttgcttatta 40 <210> 728 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 728 aggcaagtaa atttagattt catccaga 28 <210> 729 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 729 ttggattttg tcaaagacaa atggtca 27 <210> 730 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 730 tggattttgt caaagacaaa tggtcg 26 <210> 731 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 731 acgagtccat caaagatgag tggag 25 <210> 732 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 732 ctcacctttg acggatttgg at 22 <210> 733 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 733 caaaactgca aaatgcacat ggg 23 <210> 734 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 734 tcaaaactgc aaaatgcaca tgga 24 <210> 735 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 735 gtgcaaacga aaaaactggc aaca 24 <210> 736 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 736 ttggatggat tgatgcagtc aaa 23 <210> 737 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 737 aatgacaatt aagcacaaac ctgaaagata 30 <210> 738 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 738 tgacaattaa gcacaaacct gaaagatg 28 <210> 739 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 739 gaggctgcca cttgcaca 18 <210> 740 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 740 gcattaggaa gaagccaaat gacaat 26 <210> 741 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 741 gctggcatcc gacgaagg 18 <210> 742 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 742 gctggcatcc gacgaaga 18 <210> 743 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 743 agtgtttccg ttgtgctgcc 20 <210> 744 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 744 agaagtacaa actcattgac accg 24 <210> 745 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 745 cgtaccatca ttcattgatt catgcg 26 <210> 746 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 746 cgtaccatca ttcattgatt catgca 26 <210> 747 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 747 tggacaatgg tgacccgatg t 21 <210> 748 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 748 ccttatgcaa caactcagcg tac 23 <210> 749 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 749 ttttgtctgg tccatttttg ctgt 24 <210> 750 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 750 ttgtctggtc catttttgct gc 22 <210> 751 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 751 atcccctgac tccacgcttt 20 <210> 752 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 752 tggtataaat gttacccaat ttcggttt 28 <210> 753 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 753 aggtttggag gagcagaaca tatc 24 <210> 754 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 754 aggtttggag gagcagaaca tatt 24 <210> 755 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 755 tcaagttctg ctcatgtaat tgaatctcat ca 32 <210> 756 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 756 tttctagttt ctgctcattt tcaaggt 27 <210> 757 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 757 tgaaccttct tatgctcagc gtaag 25 <210> 758 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 758 tgaaccttct tatgctcagc gtaaa 25 <210> 759 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 759 ggttccaaac aacaagcacc aaac 24 <210> 760 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 760 agtgaataat catgcttttg aaccttct 28 <210> 761 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 761 ggaatttctt gtgctcatcg ct 22 <210> 762 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 762 ggaatttctt gtgctcatcg cc 22 <210> 763 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 763 tggcagaatc gtaataatct atttaacgag gg 32 <210> 764 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 764 atggtgggcc gcagg 15 <210> 765 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 765 cagcaacttg agggaagcg 19 <210> 766 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 766 tcagcaactt gagggaagca 20 <210> 767 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 767 ctccttttcc acctctaact agacgg 26 <210> 768 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 768 cactgaaaga actagaacac atggt 25 <110> Seoul National University R & DB Foundation <120> SNP marker set for discrimination of subspecies in rice and uses          about <130> PN18361 <160> 768 <170> KoPatentIn 3.0 <210> 1 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 1 gacatttgca agcaacttat actgaca 27 <210> 2 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 2 acatttgcaa gcaacttata ctgacc 26 <210> 3 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 3 tgcttcagag atggcctcga a 21 <210> 4 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 4 gatgatacag gtacaaatga catttgc 27 <210> 5 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 5 gaaatacgcc ggccaaca 18 <210> 6 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 6 gaaatacgcc ggccaacg 18 <210> 7 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 7 tgagaggaag cttggaaagg gag 23 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 8 ttctgttacc atgagtagga gagg 24 <210> 9 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 9 cgtgttaaaa atagagggca ttgaatcaa 29 <210> 10 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 10 cgtgttaaaa atagagggca ttgaatcag 29 <210> 11 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 11 gccctactta ttctatacac caggct 26 <210> 12 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 12 ggtttcccac ttacataaac gtgttaa 27 <210> 13 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 13 gtgctcatct ttagattgat ttgtccat 28 <210> 14 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 14 gtgctcatct ttagattgat ttgtccag 28 <210> 15 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 15 gatggcactt cacattggcc t 21 <210> 16 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 16 gaaccatctt tgaggtgctc at 22 <210> 17 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 17 tctcaattta acaacagcat ctgaagc 27 <210> 18 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 18 gatctcaatt taacaacagc atctgaaga 29 <210> 19 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 19 ccgttgcttt gttggtcgga 20 <210> 20 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 20 aggaaatggg atttctaaat gatctcaa 28 <210> 21 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 21 aatagcatct aaaaacatgt tttacaccga a 31 <210> 22 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 22 agcatctaaa aacatgtttt acaccgag 28 <210> 23 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 23 cggagggatg aaaatttaat ttttctctcc aa 32 <210> 24 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 24 catgaatatg ctaataggaa caatagcatc t 31 <210> 25 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 25 caggctggac attttgactg ac 22 <210> 26 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 26 tcaggctgga cattttgact gat 23 <210> 27 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 27 gtcaagaatc tctgtaagaa atagacaata tgtcagta 38 <210> 28 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 28 gagtcagttt atatggtttc aggct 25 <210> 29 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 29 cgctagtgta gagcacgaac atg 23 <210> 30 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 30 cgctagtgta gagcacgaac atc 23 <210> 31 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 31 ggaccacggt gagccaga 18 <210> 32 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 32 gagatttaca acaagctatg caacg 25 <210> 33 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 33 agggacagat gctgctttgt taa 23 <210> 34 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 34 gggacagatg ctgctttgtt ag 22 <210> 35 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 35 ccgcaacagt aaccagcaca a 21 <210> 36 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 36 tgcgtacttt acgatgaagg ga 22 <210> 37 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 37 gcttcaggat ttcagtagga tccac 25 <210> 38 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 38 gcttcaggat ttcagtagga tccag 25 <210> 39 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 39 cccgctgaag aacgtgctg 19 <210> 40 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 40 tcactagtca tcactaaatc agagctt 27 <210> 41 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 41 gttgatggtc cttgctaaaa gtgg 24 <210> 42 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 42 agttgatggt ccttgctaaa agtga 25 <210> 43 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 43 atcatcacct tcagatttca aacaaaaaga ca 32 <210> 44 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 44 agcacctaat atgaaaagtt gatggtc 27 <210> 45 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 45 agcaatcctg agggccataa c 21 <210> 46 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 46 agcaatcctg agggccataa t 21 <210> 47 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 47 cgtctcatgg caaaggctga 20 <210> 48 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 48 gtcagatatc ctagcagcct ca 22 <210> 49 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 49 ctggtgctgg tttaagcagt aa 22 <210> 50 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 50 ctggtgctgg tttaagcagt ag 22 <210> 51 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 51 ctagaatcat cgtaattact actcatatat gtcaccattt 40 <210> 52 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 52 tcattcttaa ttttactgct ggtgct 26 <210> 53 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 53 aggtggctca cttgaactgt at 22 <210> 54 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 54 aggtggctca cttgaactgt ag 22 <210> 55 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 55 ggaggttcgg ccaaacgttc 20 <210> 56 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 56 gcctatatag aagcttcgat cgatca 26 <210> 57 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 57 ttctgaaact tagcagccag gtaa 24 <210> 58 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 58 tctgaaactt agcagccagg tag 23 <210> 59 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 59 gccccaatac atagctgctg g 21 <210> 60 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 60 gcacaggaag cttgacgttc 20 <210> 61 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 61 acaccgataa acacgttatg acacat 26 <210> 62 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 62 caccgataaa cacgttatga cacac 25 <210> 63 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 63 cccagcagta gcagatccag a 21 <210> 64 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 64 gcataattag catactatga caccga 26 <210> 65 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 65 gatcgatcac ctcgctgct 19 <210> 66 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 66 atcgatcacc tcgctgcc 18 <210> 67 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 67 cgctggtcgc tgccg 15 <210> 68 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 68 cggaggaaaa cacagtagga tc 22 <210> 69 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 69 cagacaatct tcaggcaaag ctc 23 <210> 70 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 70 cagacaatct tcaggcaaag ctt 23 <210> 71 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 71 agtctgaaga tgccgagggt c 21 <210> 72 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 72 atccgaatca gatgaaagat tgca 24 <210> 73 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 73 agtagaaaat gtattcacag ttccagct 28 <210> 74 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 74 agtagaaaat gtattcacag ttccagca 28 <210> 75 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 75 aaaaagttct ggccgtcgca a 21 <210> 76 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 76 gcgtgtaacg ttttatgcag tagaa 25 <210> 77 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 77 tgatacgatt acaggacgtg tactact 27 <210> 78 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 78 tgatacgatt acaggacgtg tactaca 27 <210> 79 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 79 actacaaacc taactaatcg gagatgaaaa tcg 33 <210> 80 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 80 tgatctggac aataatagtc atgatacga 29 <210> 81 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 81 ccgtgtactc gcatgtgaca at 22 <210> 82 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 82 cgtgtactcg catgtgacaa c 21 <210> 83 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 83 gggtgtcagt ttgcccttgc 20 <210> 84 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 84 ttgaggcatg ttcttgccg 19 <210> 85 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 85 tgcctttaat ggagcatcat gga 23 <210> 86 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 86 gcctttaatg gagcatcatg gg 22 <210> 87 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 87 ggattgcatg accatcaagg ca 22 <210> 88 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 88 cccttgctca atttgctgtt g 21 <210> 89 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 89 gttgtttgac tgtacccaaa tgtact 26 <210> 90 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 90 gttgtttgac tgtacccaaa tgtacc 26 <210> 91 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 91 agcaacatct atagttgtcc agggatttt 29 <210> 92 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 92 aaaggtggtg ctatgttgtt gt 22 <210> 93 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 93 gatccgggtg gtgtcagtt 19 <210> 94 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 94 gatccgggtg gtgtcagtc 19 <210> 95 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 95 cagagtaatg ggaaggcatg ca 22 <210> 96 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 96 gagattttac ttgagaaagc agatctctt 29 <210> 97 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 97 cctgttctac cgtcaagcga c 21 <210> 98 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 98 cctgttctac cgtcaagcga t 21 <210> 99 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 99 tccgtgatga tgctaatttt tatttatttt tactaattgt 40 <210> 100 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 100 gatgtacacc atccaatttt acctgt 26 <210> 101 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 101 gaagaagatg ccaagtttgc tgtt 24 <210> 102 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 102 aagaagatgc caagtttgct gtc 23 <210> 103 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 103 agggaagctt cagccatcgt 20 <210> 104 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 104 gacttgcctg aatcctcaga a 21 <210> 105 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 105 tcaaagattc ggatggttcc ca 22 <210> 106 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 106 tcaaagattc ggatggttcc cg 22 <210> 107 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 107 gctgaagctg atccattgca ca 22 <210> 108 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 108 gctgagttgt tcaactcaag ct 22 <210> 109 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 109 ttgccactgt gtgcaacatt t 21 <210> 110 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 110 ttgccactgt gtgcaacatt c 21 <210> 111 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 111 catcggcaac gagacgaaac a 21 <210> 112 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 112 gcatgtgtga atacatggcg t 21 <210> 113 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 113 tcaatctgca catacgggat aaatgt 26 <210> 114 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 114 caatctgcac atacgggata aatgc 25 <210> 115 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 115 ggcgatatat tctcttcatt catgctaaag tg 32 <210> 116 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 116 ctgaggagag atatatcctc tgtcaat 27 <210> 117 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 117 agttaaacaa gtagttactc taaccgttga a 31 <210> 118 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 118 agttaaacaa gtagttactc taaccgttga t 31 <210> 119 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 119 actagctaga aagaaatctc tgactttttg tgt 33 <210> 120 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 120 ggctccatgc atatctatag ttaaacaa 28 <210> 121 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 121 cattccgtgt gaaccacgtt tg 22 <210> 122 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 122 cattccgtgt gaaccacgtt tc 22 <210> 123 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 123 gtgctccaaa tttgccttgg tga 23 <210> 124 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 124 cctccattac ctatgacaaa cacattc 27 <210> 125 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 125 ctcctcgcag aacggctac 19 <210> 126 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 126 ctcctcgcag aacggctaa 19 <210> 127 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 127 cgtccaaggc gctcttgtc 19 <210> 128 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 128 gacggctccg acgatga 17 <210> 129 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 129 ctgaaataga aagggctgtt ttggt 25 <210> 130 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 130 tgaaatagaa agggctgttt tggc 24 <210> 131 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 131 aacataactc tagcaaaaga attggtaggg ta 32 <210> 132 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 132 ggaacatgta tggagtatag tactgaaat 29 <210> 133 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <133> 133 catgccccta attttgcaga act 23 <210> 134 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 134 catgccccta attttgcaga aca 23 <210> 135 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 135 caactgctga gaatttaaat cccatcca 28 <210> 136 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 136 gttagggtgt gcaccaaaca 20 <210> 137 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 137 catgcgttgg tcattgccta c 21 <210> 138 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 138 catgcgttgg tcattgccta a 21 <139> <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 139 agtatggcaa accgggagca 20 <210> 140 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 140 catcacattt ttacggggtt atacca 26 <210> 141 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 141 gcttgctggt ttgtcaagtg c 21 <210> 142 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 142 gcttgctggt ttgtcaagtg t 21 <210> 143 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 143 agtttcttta tcttcgcatc ggatgga 27 <210> 144 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 144 ggattgttgt ggacaatttc aactg 25 <210> 145 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 145 ttcaggtact gatgccaagt acc 23 <210> 146 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 146 gattcaggta ctgatgccaa gtact 25 <210> 147 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 147 tgtggactag ccctacgtac tct 23 <210> 148 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 148 tctaaatctg atggtccctg attca 25 <210> 149 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 149 agcagcctcc tcttctgtg 19 <210> 150 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 150 cagcagcctc ctcttctgtt 20 <210> 151 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 151 atgcgaagct tgggcgtg 18 <210> 152 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 152 cccgcctgct ccaaatac 18 <210> 153 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 153 ttccttttca ctgcattaca aatcagac 28 <210> 154 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 154 cttccttttc actgcattac aaatcagat 29 <210> 155 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 155 ggaatatcat catattatag gttgactagc tactcc 36 <210> 156 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 156 ctttcgatcc agtacttcct tttca 25 <210> 157 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 157 accaaactat tgttgtatag gagtaagagc 30 <210> 158 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 158 caccaaacta ttgttgtata ggagtaagag t 31 <210> 159 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 159 cggccagggt tctgtcaaa 19 <210> 160 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 160 cagatcaaca aagtgacacc aaacta 26 <210> 161 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 161 cgtgcaagac catttgaagc tc 22 <210> 162 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 162 cgtgcaagac catttgaagc ta 22 <210> 163 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 163 gggcgccaac accaaaacc 19 <210> 164 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 164 gacaacagcc atgatggacc 20 <210> 165 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 165 gcctgaaaat gcttgtttct ctgt 24 <210> 166 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 166 gcctgaaaat gcttgtttct ctga 24 <210> 167 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 167 tgttcattaa ccgatcggat tccgaat 27 <210> 168 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 168 caaaggtaca tacatataca tctctagcc 29 <210> 169 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 169 tggcaaaggt aaggcctcac 20 <210> 170 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 170 tggcaaaggt aaggcctcaa 20 <210> 171 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 171 cagtgggcaa tcgagcgt 18 <210> 172 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 172 agctcaggga ggtggc 16 <210> 173 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 173 gaactgtccc tttcttggtc atc 23 <210> 174 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 174 ggaactgtcc ctttcttggt catt 24 <175> 175 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 175 gacgcgtgct tgacagcat 19 <210> 176 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 176 aaggcgttga tcagaatgga ac 22 <210> 177 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 177 gccaaacttt gtcagaaaca ggtt 24 <210> 178 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 178 gccaaacttt gtcagaaaca ggtc 24 <210> 179 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 179 gcatgatact tctatcatgt acactcctga 30 <210> 180 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 180 cttcagtact gttagtaacg ttgcc 25 <210> 181 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 181 gcaagccgat tgttacgaac tt 22 <210> 182 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 182 gcaagccgat tgttacgaac tc 22 <210> 183 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 183 gctttgctat gtggttggtg ataca 25 <210> 184 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 184 cgcaaccttc gttagcagtg 20 <210> 185 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 185 agtttcatag atcaaccagt tcgagt 26 <210> 186 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 186 gtttcataga tcaaccagtt cgagc 25 <210> 187 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 187 agatactcga agcgtctgtg ttctttt 27 <210> 188 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 188 ttgccactaa cttgaagcag ttt 23 <210> 189 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 189 cgtaataatg atataataac cgcgctgaat tac 33 <210> 190 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 190 cgtaataatg atataataac cgcgctgaat taa 33 <210> 191 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 191 gctgttgtga tgcgtgggat 20 <210> 192 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 192 gtactccggc ttacgtaata atgat 25 <210> 193 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 193 aaaaaaccta cctgatcaga tccact 26 <210> 194 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 194 aaaaacctac ctgatcagat ccaca 25 <210> 195 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 195 gagatgatat tttatagtgt agtcttacca tcaatttctt 40 <210> 196 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 196 aacctatctc acacacaaaa aacct 25 <210> 197 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 197 acaatccatg gtcggttgat gaa 23 <210> 198 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 198 acaatccatg gtcggttgat gat 23 <210> 199 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 199 gcctgcatga ccatcggc 18 <210> 200 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 200 cccaggacga ctgcac 16 <210> 201 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 201 aacagaagct acaacttcgt gagat 25 <210> 202 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 202 acagaagcta caacttcgtg agac 24 <210> 203 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 203 catctaaaag aaatgcattg ttgctttggc 30 <210> 204 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 204 gcctgatagg tagaggacaa aaca 24 <210> 205 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 205 gtcaaatttc ggcctgttaa ccc 23 <206> 206 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 206 gtcaaatttc ggcctgttaa cct 23 <210> 207 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 207 ccccacaaat acttagcagg cc 22 <210> 208 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 208 ctccaaaaac ctgaaaattt gagtgt 26 <210> 209 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 209 ctcaggaagg ttatcgttgc cta 23 <210> 210 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 210 tcaggaaggt tatcgttgcc tg 22 <210> 211 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 211 caccaactat tcagaatatc gcaagaattt tga 33 <210> 212 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 212 gccgaacaaa atcattcttc aaaaact 27 <210> 213 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 213 caaaatccct tatattattg ctattaaacc tttgat 36 <210> 214 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 214 caaaatccct tatattattg ctattaaacc tttgac 36 <210> 215 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 215 aattacatta ctgtatattt tatattccta tgttgtcaaa 40 <210> 216 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 216 gcaagcaaaa actcaaaatc ccttatat 28 <210> 217 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 217 gccacacata cacatcagca taca 24 <210> 218 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 218 ccacacatac acatcagcat acg 23 <210> 219 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 219 aaaaattcaa atagaaatat atatatcctg tgagcaaaca 40 <210> 220 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 220 acaacaataa cataactgac ctgcc 25 <210> 221 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 221 ggttggtttg gcgatctcat ct 22 <210> 222 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 222 gttggtttgg cgatctcatc a 21 <210> 223 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 223 aggtgctcca gtgctgtaag g 21 <210> 224 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 224 ccatggcttg ttacaagtta cagtg 25 <210> 225 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 225 actgatgtga agaacagtct agagc 25 <210> 226 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 226 cactgatgtg aagaacagtc tagagt 26 <210> 227 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 227 tgcttcatgc catcgatcag ga 22 <210> 228 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 228 gtaggcaaac tgtagtgtca ctg 23 <210> 229 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 229 cggaatctca ttttcttaag ctgcata 27 <210> 230 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 230 cggaatctca ttttcttaag ctgcatt 27 <210> 231 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 231 gtaagaaaat atattggtct attagcctat acaatcctgt 40 <210> 232 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 232 tcatcaattc aacacggaat ctcatt 26 <210> 233 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 233 acgtggctca agctcactg 19 <210> 234 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 234 aacgtggctc aagctcacta 20 <210> 235 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 235 tgcgcaaatc gcagtgtgac 20 <210> 236 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 236 cttgtggagg tggtgca 17 <210> 237 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 237 cataaatcag aattacaggt tgcagct 27 <210> 238 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 238 cataaatcag aattacaggt tgcagca 27 <210> 239 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 239 aaaccttgga ggaatgtcat cgtct 25 <210> 240 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 240 ttcacagttt cacacaggac ataaa 25 <210> 241 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 241 aggcaaaatt ggcagccaaa 20 <210> 242 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 242 aggcaaaatt ggcagccaac 20 <210> 243 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 243 agtgctcatt gggaattttg taattctttg tt 32 <210> 244 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 244 catacctaaa aaggcaaaat tggca 25 <210> 245 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 245 atcagcttga ggtagcttca tctc 24 <210> 246 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 246 tcagcttgag gtagcttcat ctg 23 <210> 247 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 247 gcaggcaaag atgattctta tttattagac cc 32 <210> 248 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 248 tgatatgcag aatgtgtgcc atc 23 <210> 249 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 249 ccaacgggtc gctggtg 17 <210> 250 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 250 ccaacgggtc gctggta 17 <210> 251 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 251 acaggagttc atcatagatt tgcctgt 27 <210> 252 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 252 agaccttgtg tttagcgatt tagc 24 <210> 253 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 253 tcaaggtggt cctcgacact a 21 <210> 254 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 254 caaggtggtc ctcgacactc 20 <210> 255 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 255 gtattgcgct atgccacggt 20 <210> 256 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 256 ctcaagaaag ccaccgacat tt 22 <210> 257 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 257 ggttatcgct tcagcattga atgag 25 <210> 258 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 258 ggttatcgct tcagcattga atgaa 25 <210> 259 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 259 gtgtcttgga caaccatgat ttctgg 26 <210> 260 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 260 ctgagtcata ttccagaaca tggtt 25 <210> 261 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 261 accattttac gtgacccgag aa 22 <210> 262 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 262 accattttac gtgacccgag ag 22 <210> 263 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 263 ctgtcagtgc gtggtgtgt 19 <210> 264 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 264 cagtttatag acggagggag tacc 24 <210> 265 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 265 ctcattggtg ataatgcact tgtatgaat 29 <210> 266 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 266 tcattggtga taatgcactt gtatgaac 28 <210> 267 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 267 gcattctcac aaagtgtata taatgccaga tc 32 <210> 268 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 268 gatgccagta tgtcactcat tgg 23 <210> 269 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 269 tcgttacagc atgtcagtag cc 22 <210> 270 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 270 ctcgttacag catgtcagta gca 23 <210> 271 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 271 gtgcacttcg ttccaaggct 20 <210> 272 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 272 cactgtgctg acgagttaaa atct 24 <210> 273 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 273 caaggcaagt acacttttct ccaat 25 <210> 274 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 274 caaggcaagt acacttttct ccaac 25 <210> 275 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 275 gaccacatgt aatcttagtt tacatgataa aatcgg 36 <210> 276 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 276 caagttgctg caccttttac aag 23 <210> 277 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 277 tgctcctcaa tacatttgat aacagct 27 <210> 278 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 278 gctcctcaat acatttgata acagcc 26 <210> 279 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 279 gaacgcgagt tgtctgcctt 20 <210> 280 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 280 gatatttctc atcaagtttg tgctcc 26 <210> 281 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 281 tgcattgctc cgattgatag gag 23 <210> 282 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 282 gcattgctcc gattgatagg ac 22 <210> 283 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 283 tctattctgg actacagtcc gaacatatca 30 <210> 284 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 284 gcattagaaa gtttcactat atttgtgca 29 <210> 285 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 285 aagatggtga aagccacctg a 21 <210> 286 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 286 agatggtgaa agccacctgg 20 <210> 287 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 287 agggcaacca aaagcattgg tc 22 <210> 288 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 288 cagaaaatta ggttgggacc ctaa 24 <210> 289 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 289 accacatgac ctgatctttg ca 22 <210> 290 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 290 ccacatgacc tgatctttgc g 21 <210> 291 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 291 cagctagaag aatttttgat gcaactcca 29 <210> 292 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 292 gcatatccag atatcattga ggacc 25 <210> 293 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 293 atagaagttc tgaatctgct aacactga 28 <210> 294 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 294 gaagttctga atctgctaac actgc 25 <210> 295 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 295 ttgaaatcgc cggttaccgc 20 <210> 296 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 296 cacagcaccg agcatagaag 20 <210> 297 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 297 gaacaagtcg ctgcatttgg tatta 25 <210> 298 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 298 acaagtcgct gcatttggta ttg 23 <210> 299 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 299 gcgtcaccat tggccct 17 <210> 300 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 300 tggtggccta gtacatgaac a 21 <210> 301 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 301 gctgtcacac tcgaagtgct t 21 <210> 302 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 302 gctgtcacac tcgaagtgct c 21 <210> 303 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 303 gctgacaacg aggtcggtga 20 <210> 304 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 304 ccgacctcgc tgtcac 16 <210> 305 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 305 ccaacttatc caggccatcc a 21 <210> 306 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 306 ccaacttatc caggccatcc c 21 <210> 307 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 307 ttccgcttag ccctctgctt 20 <210> 308 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 308 tggcactgtt gatttgggc 19 <210> 309 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 309 aaaccataaa gctgcttgac agattaaat 29 <210> 310 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 310 aaccataaag ctgcttgaca gattaaag 28 <210> 311 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 311 ggttacctta cattcatgca acatggaa 28 <210> 312 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 312 agcccacctc tcttaaacca ta 22 <210> 313 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 313 gaatgagtca gtcaggcagt gta 23 <210> 314 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 314 tgagtcagtc aggcagtgtg 20 <210> 315 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 315 gcaaactaaa ctaaaaaata tgtctgacag tctca 35 <210> 316 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 316 aagttggatt aacacctgaa atgaaga 27 <210> 317 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 317 agcggctatg ctatgtccg 19 <210> 318 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 318 gagcggctat gctatgtcca 20 <210> 319 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 319 ttttcttatt aaaattacag agaaccttat agcttcagtc 40 <210> 320 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 320 gctagagatg aaaacaaaat gtgagc 26 <210> 321 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 321 tctaggtgca aatggtgttg tca 23 <210> 322 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 322 ctaggtgcaa atggtgttgt cc 22 <210> 323 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 323 ggaagcatgg cactcttgaa agt 23 <210> 324 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 324 acactgatgg atctaggtgc aa 22 <210> 325 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 325 cttctcaaat cgctcaaatg atcct 25 <210> 326 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 326 cttctcaaat cgctcaaatg atcca 25 <210> 327 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 327 tccatggcca agatggggt 19 <210> 328 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 328 ggctttggca ttggcttc 18 <210> 329 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 329 atttgataaa atcgtcatcc aaaccgt 27 <210> 330 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 330 ttgataaaat cgtcatccaa accgc 25 <210> 331 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 331 ggccaacaca agatccccag 20 <210> 332 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 332 ccttatgtag atctagtaaa cactttacat ttga 34 <210> 333 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 333 gtaatcgaca ataatctcaa gttgccg 27 <210> 334 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 334 gtaatcgaca ataatctcaa gttgcca 27 <210> 335 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 335 gactgttcaa cttcagcagg ca 22 <210> 336 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 336 cagtcctgtg tcgtcgtaat c 21 <210> 337 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 337 caattttctg tgagtctttg cgga 24 <210> 338 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 338 aattttctgt gagtctttgc ggg 23 <210> 339 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 339 aagcctgagg gtgcgtaca 19 <210> 340 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 340 aaaatacaat tttctgtgag tctttgcg 28 <210> 341 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 341 gcgcgcgtgt aattttgaaa c 21 <210> 342 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 342 tgcgcgcgtg taattttgaa at 22 <210> 343 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 343 tttgacccct actctaacat gttgct 26 <210> 344 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 344 caatgctggc ctttcttttg tat 23 <210> 345 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 345 tttctgagtc ttgccctgtg t 21 <210> 346 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 346 tctgagtctt gccctgtgc 19 <210> 347 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 347 gatccttgtg ctctccagcc t 21 <210> 348 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 348 ccttggtata ccttgatctt cctttttatt tat 33 <210> 349 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 349 tgcagtagaa agatacaccg cc 22 <210> 350 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 350 atgcagtaga aagatacacc gca 23 <210> 351 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 351 gatcagatgt ggagtttttg tcacttcaa 29 <210> 352 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 352 aagactgtga agaggtcagg at 22 <210> 353 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 353 ttggcccatc gcagaagat 19 <210> 354 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 354 tggcccatcg cagaagac 18 <210> 355 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 355 tgacctctgg agtttcagtg ctc 23 <210> 356 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 356 agggcaacat ccctggg 17 <210> 357 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 357 ctcgtcacag cattagcata tactct 26 <210> 358 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 358 tcgtcacagc attagcatat actcc 25 <210> 359 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 359 aggctatgcg cctatgcca 19 <210> 360 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 360 gctttgtgaa cactaatcac tcgt 24 <210> 361 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 361 acacaccatg ctctaatggc tc 22 <210> 362 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 362 acacaccatg ctctaatggc tt 22 <210> 363 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 363 aggatcgtgg ctcagcatgt 20 <210> 364 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 364 acccaataag gcttttccaa tgaa 24 <210> 365 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 365 agagcttctt ggctccaaga at 22 <210> 366 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 366 agagcttctt ggctccaaga ac 22 <210> 367 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 367 ggccagagct acctgacct 19 <210> 368 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 368 catcccgatc ctctggca 18 <210> 369 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 369 ggagttgtgg gcttaacttt tctc 24 <210> 370 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 370 ggagttgtgg gcttaacttt tctt 24 <210> 371 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 371 ccggcctcaa gatgctcca 19 <210> 372 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 372 aggagttgtg ggcttaactt ttc 23 <210> 373 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 373 gcatcgacag agatccgaaa tgac 24 <210> 374 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 374 gcatcgacag agatccgaaa tgat 24 <210> 375 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 375 gcgacagatg aaatactgga caagc 25 <210> 376 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 376 cacattgctg ctactttttt gca 23 <210> 377 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 377 tcgtcttaaa atataatggt gcacaccta 29 <210> 378 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 378 cgtcttaaaa tataatggtg cacacctc 28 <210> 379 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 379 aaaatattat caacaatatt attttcttaa acacgcatga 40 <210> 380 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 380 tcctttttaa ctctcgtctc gtctta 26 <210> 381 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 381 ggggccactt gttttcaatc a 21 <210> 382 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 382 ggggccactt gttttcaatc c 21 <210> 383 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 383 gggaagtgtt ccctccaggt 20 <210> 384 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 384 actatggatg gcgtgcatat ga 22 <210> 385 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 385 ttatagggca tgtacacacg ct 22 <210> 386 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 386 gggcatgtac acacgcg 17 <210> 387 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 387 gctattctat aaaccttatc aggatttatg gtgaatct 38 <210> 388 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 388 gagcaactga tttgtattgt ctatttttgt 30 <210> 389 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 389 gcatatatta tttcatagga tagtccgcat ct 32 <210> 390 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 390 gcatatatta tttcatagga tagtccgcat cc 32 <210> 391 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 391 aatatgatat gaatacgaat atggtatccc taaaattcaa 40 <210> 392 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 392 gcatatatta tttcatagga tagtccgca 29 <210> 393 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 393 ctcaagtgat cttagtgcca tccta 25 <210> 394 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 394 tcaagtgatc ttagtgccat cctc 24 <210> 395 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 395 actcaatata tgcaccaatt agttctcaca tct 33 <210> 396 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 396 tcttacatgg ccaagatcat actca 25 <210> 397 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 397 cgatgccaat actagccaat gtca 24 <210> 398 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 398 gatgccaata ctagccaatg tcg 23 <210> 399 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 399 tggacatata accgattgct caaaacct 28 <210> 400 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 400 atgccgatac cagccga 17 <210> 401 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 401 cggtgtccag gtaaccgg 18 <210> 402 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 402 cggtgtccag gtaaccga 18 <210> 403 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 403 ggtcacaatc agccgtgcc 19 <210> 404 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 404 tgatcaccaa agtgttgatg cc 22 <210> 405 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 405 ttgatgctaa aaacgccagt gac 23 <210> 406 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 406 gttgatgcta aaaacgccag tgat 24 <210> 407 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 407 tgtgggtagg catcataaaa aggaact 27 <210> 408 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 408 gtactttcgt ttgatactct tcagagtt 28 <210> 409 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 409 tccacggtgg gcttgac 17 <210> 410 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 410 ttccacggtg ggcttgat 18 <210> 411 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 411 acgcccatgg acttcgagt 19 <210> 412 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 412 gcatgatgct cagcatccc 19 <210> 413 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 413 aggtgcttct gatgttagct acg 23 <210> 414 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 414 caggtgcttc tgatgttagc tact 24 <210> 415 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 415 ccaggctgtt taggagttgg c 21 <210> 416 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 416 gaccgtccat tgatgtctca g 21 <210> 417 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 417 gcatttggtg gtacgtgtat catga 25 <210> 418 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 418 catttggtgg tacgtgtatc atgc 24 <210> 419 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 419 agggttcggc gatgatggt 19 <210> 420 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 420 ccatctcgtc caaagatcct tag 23 <210> 421 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 421 catgccaagc caagatatgg g 21 <210> 422 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 422 ccatgccaag ccaagatatg ga 22 <210> 423 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 423 ccaacgagga ggagaacgca 20 <210> 424 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 424 accagactcg gtcatgcc 18 <210> 425 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 425 gcattttcga gaccagacca gg 22 <210> 426 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 426 gcattttcga gaccagacca gt 22 <210> 427 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 427 gcctaggggg cctgaaagta 20 <210> 428 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 428 acatcatgtg caaactagaa ttaagca 27 <210> 429 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 429 gggccaagga gtacacca 18 <210> 430 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 430 gggccaagga gtacacct 18 <210> 431 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 431 tctgctcggc aggcact 17 <210> 432 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 432 atcctgctct acgccgtt 18 <210> 433 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 433 gcttagctag tactacagct aatttgacat 30 <210> 434 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 434 gcttagctag tactacagct aatttgacac 30 <210> 435 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 435 tttgtttatt tagtttttta ttttgttgag gaatgaatga 40 <210> 436 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 436 ggaacaacac ctagcttagc tagt 24 <210> 437 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 437 agcttgtaga tcagctggca aat 23 <210> 438 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 438 gcttgtagat cagctggcaa aa 22 <210> 439 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 439 tgctcgtgca gtagcaggat 20 <210> 440 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 440 ctacatgaaa gtttacatgc aagtagc 27 <210> 441 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 441 cgaagatctt ccacccccg 19 <210> 442 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 442 cgaagatctt ccaccccca 19 <210> 443 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 443 acatttgtat ggtaatttga gatccgattg ttttt 35 <210> 444 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 444 ccaaccaata aggaacatct ttttcc 26 <210> 445 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 445 caacttagtg caacttttaa acatatcgaa aaa 33 <210> 446 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 446 caacttagtg caacttttaa acatatcgaa aac 33 <210> 447 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 447 gcaagcaacc aatgatctaa gataatgaag c 31 <210> 448 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 448 caactggcca acttagtgca 20 <210> 449 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 449 ggcttagcta cttcatcgtt tctagt 26 <210> 450 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 450 gcttagctac ttcatcgttt ctagc 25 <210> 451 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 451 atgcctcttt tagcagtcga ggaa 24 <210> 452 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 452 tccatttggc ttcaaggctt ag 22 <210> 453 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 453 gagtgctgtc ataatattgc actgg 25 <210> 454 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 454 gagtgctgtc ataatattgc actgc 25 <210> 455 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 455 acaacgagca attttatcat gaaaccaaca 30 <210> 456 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 456 cagcagatta atgctattcc agagt 25 <210> 457 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 457 cttgaccgca aaaccggata t 21 <210> 458 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 458 cttgaccgca aaaccggata c 21 <210> 459 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 459 ctcgtttgcc cggttttggt a 21 <210> 460 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 460 ccagtccgat tttcgtccc 19 <210> 461 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 461 ccactcaaag aaggaatcag cg 22 <210> 462 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 462 gccactcaaa gaaggaatca gca 23 <210> 463 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 463 tcaagccccg gcctcc 16 <210> 464 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 464 cgacaggagc agtgcc 16 <210> 465 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 465 gttacagcat aaaggaaagg aaaaccc 27 <210> 466 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 466 gttacagcat aaaggaaagg aaaacct 27 <210> 467 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 467 tccggctcac tgggtatgg 19 <210> 468 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 468 agcaaagtaa atggataatg ttacagca 28 <210> 469 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 469 tccacacatg tcttgtttaa aaccc 25 <210> 470 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 470 gtccacacat gtcttgttta aaacca 26 <210> 471 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 471 cctccttttt ctttctatct gtgagcatct 30 <210> 472 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 472 acatgggtgt tcgtccaca 19 <210> 473 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 473 ttgatcaaag cacacagaaa gcg 23 <210> 474 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 474 cttgatcaaa gcacacagaa agca 24 <210> 475 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 475 tgtggcgtcg atactttaca gttga 25 <210> 476 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 476 caggtgggct tgtgctt 17 <210> 477 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 477 tctgaaaata tgataagacc gggcg 25 <210> 478 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 478 tctgaaaata tgataagacc gggca 25 <210> 479 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 479 gccggttgca ttgcattctc t 21 <210> 480 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 480 ctgactttta ccctattatc gtctctg 27 <210> 481 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 481 gctctgctct ctctgtctcg a 21 <210> 482 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 482 ctctgctctc tctgtctcgg 20 <210> 483 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 483 gggacacgga cagaaaggaa aaaa 24 <210> 484 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 484 gtagcatgaa gtagtgctct gc 22 <210> 485 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 485 ctgcctccag atgctgc 17 <210> 486 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 486 gctgcctcca gatgctga 18 <210> 487 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 487 acagcaggct ggcttactct 20 <210> 488 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 488 aaattcaatc gaagggatcc acg 23 <210> 489 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 489 ggaaggctct gtgcttacgt tta 23 <210> 490 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 490 gaaggctctg tgcttacgtt tc 22 <210> 491 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 491 ccacaagcct gaatacaagg ca 22 <210> 492 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 492 tgttggagga aggctctgt 19 <210> 493 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 493 gatggcgagt ttgacacaga tcta 24 <210> 494 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 494 tggcgagttt gacacagatc tg 22 <210> 495 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 495 tgggaggtcg tcgtcgc 17 <210> 496 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 496 tggtatctgc tctatgctcg at 22 <210> 497 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 497 ccggtccaat tatccgacgt 20 <210> 498 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 498 ccggtccaat tatccgacgc 20 <210> 499 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 499 ccggggaggc taagttgct 19 <210> 500 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 500 gctcctcctg gacatcctc 19 <210> 501 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 501 tgtggaggtc caaacattgt gt 22 <210> 502 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 502 tgtggaggtc caaacattgt ga 22 <210> 503 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 503 ctaggagtct tagaatgttg atctctgaca 30 <210> 504 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 504 cagaatcttt gtggaggtcc aa 22 <210> 505 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 505 cttacaactc actctctcat gctct 25 <210> 506 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 506 ttacaactca ctctctcatg ctcc 24 <210> 507 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 507 gccatgcaaa agatttttat atagattgtg gc 32 <210> 508 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 508 tcgatgatta aaagcgaaag cttaca 26 <210> 509 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 509 tctgaagatc acgagaggaa ggt 23 <210> 510 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 510 ctgaagatca cgagaggaag gc 22 <210> 511 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 511 gttcacatgg gcctccgc 18 <210> 512 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 512 ctaaacgaga gtatgaagaa gctga 25 <210> 513 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 513 actgcacaac aatcaagatc agc 23 <210> 514 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 514 cactgcacaa caatcaagat caga 24 <210> 515 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 515 ctgactgaaa attgtgcatt gtactcact 29 <210> 516 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 516 aaccagaaag tggtgtaaca ctg 23 <210> 517 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 517 tttcgtcgtc tctatcgcca c 21 <210> 518 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 518 ctttcgtcgt ctctatcgcc at 22 <210> 519 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 519 tgacgatgat cagtgcggat ga 22 <210> 520 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 520 accgcagctt tgccac 16 <210> 521 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 521 aggtggtgtt cttttttaat taagacagat tc 32 <210> 522 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 522 aggtggtgtt cttttttaat taagacagat tt 32 <210> 523 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 523 accatttgac agtttgaaaa ggatggtaat aaaaa 35 <210> 524 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 524 gcagaacata aggtggtgtt ctt 23 <210> 525 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 525 tggagttgaa agttgaaact catcaac 27 <210> 526 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 526 gtggagttga aagttgaaac tcatcaat 28 <210> 527 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 527 ggaggtcagt caaatttcta taatctttag aagca 35 <210> 528 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 528 tggagttgaa agttgaaact catca 25 <210> 529 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 529 tcagtgtggc tggaagaagt c 21 <210> 530 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 530 catcagtgtg gctggaagaa gta 23 <210> 531 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 531 gcaagtcacc tctttgttgt tttaacga 28 <210> 532 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 532 tgcagtgtct tcctcagca 19 <210> 533 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 533 gctggatcta tagcccccc 19 <210> 534 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 534 tgctggatct atagccccct 20 <210> 535 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 535 aaatccaaca cgccggaagc 20 <210> 536 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 536 gcttacgcgt ccttttcgt 19 <210> 537 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 537 aaagccacca gcaattgtca ttt 23 <210> 538 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 538 aagccaccag caattgtcat tc 22 <210> 539 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 539 acgtcggaca acaaattgaa tgaaaatatg at 32 <540> 540 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 540 agcaagacac ataaaattga gcctaa 26 <210> 541 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 541 tctgtatctc actgtagtcc tggt 24 <210> 542 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 542 tctgtatctc actgtagtcc tggg 24 <210> 543 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 543 ggatctcaca gtagcctatc aaatgga 27 <210> 544 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 544 ctggagcatt gctgcttct 19 <210> 545 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 545 caaaacggcg gcagaattgt 20 <210> 546 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 546 aaacggcggc agaattgc 18 <210> 547 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 547 gacaaagcta ggattattag atatgatcac caagt 35 <210> 548 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 548 aatgtggcag ctgacacac 19 <210> 549 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 549 aaagctccta tgcaccccta at 22 <210> 550 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 550 aaagctccta tgcaccccta ag 22 <210> 551 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 551 ccatctgctt gatagactaa ttgggca 27 <210> 552 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 552 gctggtacat gagtaccatc ca 22 <210> 553 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 553 catctggcaa cttctgttct atactagta 29 <210> 554 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 554 catctggcaa cttctgttct atactagtt 29 <210> 555 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 555 cagctcgatc gaggatgata gaaca 25 <210> 556 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 556 gttcccttct tcatctggca a 21 <210> 557 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 557 gcgtgccaat attcctgtag tg 22 <210> 558 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 558 atgcgtgcca atattcctgt agta 24 <210> 559 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 559 tgcggaacgc agcctaacta 20 <210> 560 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 560 attatttttt gaacatgcgt gccaa 25 <210> 561 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 561 ggatacatgt gtgcgagtgc a 21 <210> 562 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 562 gatacatgtg tgcgagtgcg 20 <210> 563 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 563 gggaagaaca gggccacg 18 <210> 564 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 564 gtcaaccgat gaggttgtac ttt 23 <210> 565 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 565 gtatatgatt cccctgacca ggaataag 28 <210> 566 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 566 gtatatgatt cccctgacca ggaataat 28 <210> 567 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 567 ggcattagca gcatctttcc tttgt 25 <210> 568 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 568 cgagccatgc agcagtatat g 21 <210> 569 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 569 cagccgtcca aacggttc 18 <210> 570 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 570 cagccgtcca aacggttt 18 <210> 571 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 571 gtacagcaaa ctcttctata tatgatctgt tcgt 34 <210> 572 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 572 acgccgtcct gatcatcg 18 <210> 573 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 573 tgtacgttgt ccaaatacat ctctagaaa 29 <210> 574 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 574 atgtacgttg tccaaataca tctctagaat 30 <210> 575 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 575 gcatgtcctg aagtactact cctgt 25 <210> 576 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 576 gaggatgtat atctttgaca tgtacgtt 28 <210> 577 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 577 gggagggaaa ggactccc 18 <210> 578 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 578 agggagggaa aggactcca 19 <210> 579 <211> 39 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 579 agtattaaac aaacagaaga aaggataatg atactctgt 39 <210> 580 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 580 cgctgctgct gcatcaaa 18 <210> 581 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 581 gcttcttttg ttaatgatga ttagttcata cagta 35 <210> 582 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 582 gcttcttttg ttaatgatga ttagttcata cagtc 35 <210> 583 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 583 cacctttctt tcttctttct tttctctgaa tgt 33 <210> 584 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 584 gtgcttcttt tgttaatgat gattagttca 30 <210> 585 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 585 tcatcaggaa gaacatctgc aagt 24 <210> 586 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 586 catcaggaag aacatctgca agg 23 <210> 587 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 587 tcgatctgaa taagagggga aacaaagaat 30 <210> 588 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 588 tgcttgtgtt gttctgttgt tca 23 <210> 589 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 589 ccgaattagg cataaatttg gcacg 25 <210> 590 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 590 ccgaattagg cataaatttg gcacc 25 <210> 591 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 591 tgctgcacag attgcacgac 20 <210> 592 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 592 tgatcatcag ctaattccac cga 23 <210> 593 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 593 tgaggaataa tcgacgatca gcaaa 25 <210> 594 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 594 tgaggaataa tcgacgatca gcaag 25 <210> 595 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 595 ccaggggaaa caattcggct t 21 <210> 596 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 596 cgcaagctat aagatatcat aggatgag 28 <210> 597 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 597 ctgatactaa gggaaacaaa atgcatgaa 29 <210> 598 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 598 tgatactaag ggaaacaaaa tgcatgac 28 <210> 599 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 599 ctcctgggca ttggcatgaa 20 <210> 600 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 600 ctccagaggt aatttttgga ccat 24 <210> 601 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 601 tgaagcatgg ccttgaagac t 21 <210> 602 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 602 gaagcatggc cttgaagacc 20 <210> 603 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 603 acagtgtgtg gagatcaagg acat 24 <210> 604 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 604 cggtgatgta ccagtgtaaa tgaaa 25 <210> 605 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 605 aaattgtgaa ggacaaaaac atccgtta 28 <210> 606 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 606 ttgtgaagga caaaaacatc cgttt 25 <210> 607 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 607 agtgctgtga tgagtagcaa tcattga 27 <210> 608 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 608 ccaagtgaaa aatggtaaga aattgtga 28 <210> 609 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 609 cttactatgc tcccaaatta agcgtc 26 <210> 610 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 610 tcttactatg ctcccaaatt aagcgtt 27 <210> 611 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 611 accagggcac gcaggt 16 <210> 612 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 612 acaagagtaa tactccactg ttcttact 28 <210> 613 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 613 tccacacgga cacggtttc 19 <210> 614 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 614 tccacacgga cacggtttt 19 <210> 615 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 615 acaaaacaat aatggaatgg atctgaaaag tgt 33 <210> 616 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 616 acacactcaa agagaagatc tatggt 26 <210> 617 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 617 tgaacttatt ccttcacatt attgccaac 29 <210> 618 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 618 gtgaacttat tccttcacat tattgccaat 30 <210> 619 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 619 agcacgagca cgagaactca 20 <210> 620 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 620 agggacctag agtgaactta ttcc 24 <210> 621 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 621 caatgctgga gtccatatca aaatcaa 27 <210> 622 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 622 caatgctgga gtccatatca aaatcat 27 <210> 623 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 623 cttgttcctt gatattttag atcttaatta actttgtgta 40 <210> 624 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 624 cccgatcgta atgcaatgct 20 <210> 625 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 625 acctgtactc ctgtaggttt aggtt 25 <210> 626 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 626 tacctgtact cctgtaggtt taggta 26 <210> 627 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 627 cgtcttgcca agcaagcca 19 <210> 628 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 628 ctagctagct actgtacatg tacct 25 <210> 629 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 629 gattcattta gctcagctta gctgtg 26 <210> 630 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 630 gattcattta gctcagctta gctgtc 26 <210> 631 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 631 cccacactag ctgactgtcc t 21 <210> 632 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 632 cggcctccat cttaatcgat tc 22 <210> 633 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 633 ccccaaacta ttacagttgc tcg 23 <210> 634 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 634 accccaaact attacagttg ctca 24 <210> 635 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 635 gggtgttaaa tatctagtat ttgagtttaa ggaggta 37 <210> 636 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 636 gggaaaaaag aagtttgaat tacccc 26 <637> 637 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 637 aattaatgtt tgtcctcacc tatttcgttt ag 32 <210> 638 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 638 aattaatgtt tgtcctcacc tatttcgttt aa 32 <210> 639 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 639 tttacgtggt tttttcaaag aaaataataa taatgaagga 40 <210> 640 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 640 atgtttgtcc tcacctattt cgttt 25 <210> 641 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 641 tgctctttct tctgctttga ctttg 25 <210> 642 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 642 gtgctctttc ttctgctttg actttt 26 <210> 643 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 643 gcgaatggac acggcagc 18 <210> 644 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 644 cgtcaatgca ttgcagtgct 20 <210> 645 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 645 caaagccttc tcaagggaag aac 23 <210> 646 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 646 caaagccttc tcaagggaag aag 23 <210> 647 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 647 gcttcttgga ctcttcgaga cct 23 <210> 648 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 648 tcctggagga agaacaagaa ca 22 <210> 649 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 649 actgacaaat acctgccaca ca 22 <210> 650 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 650 ctgacaaata cctgccacac g 21 <210> 651 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 651 tcaaactcca acttttattt tgaacactgc t 31 <210> 652 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 652 cataccgtta acatgcaaaa caact 25 <210> 653 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 653 cagataacag ggacatagtg gacata 26 <654> 654 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 654 agataacagg gacatagtgg acatg 25 <210> 655 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 655 gagccgccta agattttcct ttcg 24 <210> 656 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 656 ggccgaccta tggatatata cagat 25 <210> 657 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 657 gcagtgggat ggcagc 16 <210> 658 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 658 tgcagtggga tggcagt 17 <210> 659 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 659 cggcgctttg ctgcagg 17 <210> 660 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 660 tggtggtatc tcatcgtatt tttgaag 27 <210> 661 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 661 ctatataaaa aaagaggggc gatcctaaac 30 <210> 662 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 662 tctatataaa aaaagagggg cgatcctaaa a 31 <210> 663 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 663 tctttcttaa gataattcat agcataatag gaggaggt 38 <210> 664 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 664 ctatataaaa aaagaggggc gatcctaa 28 <665> 665 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 665 atctctagta aaagggtgcc atacg 25 <210> 666 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 666 gatctctagt aaaagggtgc cataca 26 <210> 667 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 667 agcctatagc cataatgatg tcattgtga 29 <210> 668 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 668 catactacag ccatggaaat gatct 25 <210> 669 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 669 ccacaaagac actgacacca att 23 <210> 670 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 670 ccacaaagac actgacacca atc 23 <210> 671 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 671 ctgcccccgg ccaatg 16 <210> 672 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 672 gtgtcaggac aggtgaatcc 20 <210> 673 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 673 tcttccacca aacttgcatg aattc 25 <210> 674 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 674 tcttccacca aacttgcatg aattt 25 <210> 675 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 675 agaaaatacc atcctcaagg agcaca 26 <210> 676 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 676 actccaaatt tgttgcacct ctt 23 <210> 677 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 677 ctcaaggccg tacatccata tgta 24 <210> 678 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 678 tcaaggccgt acatccatat gtg 23 <210> 679 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 679 ccttctctaa tattcacgtc ttttagattt acgct 35 <210> 680 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 680 gatagttgct tactcttctc aaggc 25 <210> 681 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 681 atctaaaggc gagcaaagag gt 22 <210> 682 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 682 ctaaaggcga gcaaagaggc 20 <210> 683 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 683 tcctttagct cggcaactcg t 21 <210> 684 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 684 gatgatagta ataaatctaa aggcgagca 29 <210> 685 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 685 tgagcccgag cttgacataa 20 <210> 686 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 686 tgagcccgag cttgacatac 20 <210> 687 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 687 gctgaggctc aaagctaggg t 21 <210> 688 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 688 ccgatcccct ctcgtgt 17 <210> 689 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 689 atggatcaac aaaattgtgc tcctattt 28 <210> 690 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 690 gatggatcaa caaaattgtg ctcctatta 29 <210> 691 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 691 caaagtgagc aagggtcagc a 21 <210> 692 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 692 ctaaacacca gggatatgat ggatc 25 <210> 693 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 693 gcccaagcac actcactaat tttta 25 <210> 694 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 694 gcccaagcac actcactaat ttttt 25 <210> 695 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 695 tactagtatt aaactatagg ggattagtgt agatcatttt 40 <210> 696 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 696 ggctggagta aaatctgccc 20 <210> 697 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 697 ctactcctac gtgttctgtt ccaat 25 <210> 698 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 698 actcctacgt gttctgttcc aac 23 <210> 699 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 699 gctctctgaa atagtatacg tgtaaagctt agt 33 <210> 700 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 700 ctttctctgt gttgtgtgct act 23 <210> 701 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 701 agaaattccc tttcaccttt tgcg 24 <210> 702 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 702 aagaaattcc ctttcacctt ttgca 25 <210> 703 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 703 agcgtgagga tggatccaac a 21 <210> 704 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 704 caatacgtct tgatcaggaa ctgac 25 <210> 705 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 705 aactgcaatt ctaggagtgg ct 22 <210> 706 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 706 aactgcaatt ctaggagtgg ca 22 <210> 707 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 707 cgaggctatg ttatgttcta tgttcatgac t 31 <210> 708 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 708 gaacatgggc ataatctcat atagaca 27 <210> 709 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 709 ctatatatta ttattaattg cttacacctc gttttc 36 <210> 710 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 710 ctatatatta ttattaattg cttacacctc gttttt 36 <210> 711 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 711 gtggtcaaat taaagtcaca tttaaaatac catacca 37 <210> 712 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 712 ctcaccagct tgctgtacta tatattatta tt 32 <210> 713 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 713 cgaaagagag ggactactat ttatttatga tgtc 34 <210> 714 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 714 cgaaagagag ggactactat ttatttatga tgta 34 <210> 715 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 715 tggacggagc tagcattgtc at 22 <210> 716 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 716 catcctctaa tacgaaagag aggga 25 <210> 717 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 717 tgagggcaat gcatttgtga tacta 25 <210> 718 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 718 agggcaatgc atttgtgata ctg 23 <210> 719 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 719 ccatcaataa tgcatggagt catgatgag 29 <210> 720 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 720 gctaacacct gattggttag ctt 23 <210> 721 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 721 gttgcgtgaa gacctcagaa attta 25 <210> 722 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 722 ttgcgtgaag acctcagaaa tttg 24 <210> 723 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 723 gttgttttct tcccggtata aatcactgt 29 <210> 724 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 724 acaaccttga ggtgttgcg 19 <210> 725 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 725 aatttagatt tcatccagaa ttgagttgca 30 <210> 726 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 726 aaatttagat ttcatccaga attgagttgc t 31 <210> 727 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 727 ctggattgat tgatatatag atatgtttga ttgcttatta 40 <210> 728 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 728 aggcaagtaa atttagattt catccaga 28 <210> 729 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 729 ttggattttg tcaaagacaa atggtca 27 <210> 730 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 730 tggattttgt caaagacaaa tggtcg 26 <210> 731 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 731 acgagtccat caaagatgag tggag 25 <210> 732 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 732 ctcacctttg acggatttgg at 22 <210> 733 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 733 caaaactgca aaatgcacat ggg 23 <210> 734 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 734 tcaaaactgc aaaatgcaca tgga 24 <210> 735 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 735 gtgcaaacga aaaaactggc aaca 24 <210> 736 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 736 ttggatggat tgatgcagtc aaa 23 <210> 737 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 737 aatgacaatt aagcacaaac ctgaaagata 30 <210> 738 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 738 tgacaattaa gcacaaacct gaaagatg 28 <210> 739 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 739 gaggctgcca cttgcaca 18 <210> 740 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 740 gcattaggaa gaagccaaat gacaat 26 <210> 741 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 741 gctggcatcc gacgaagg 18 <210> 742 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 742 gctggcatcc gacgaaga 18 <210> 743 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 743 agtgtttccg ttgtgctgcc 20 <210> 744 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 744 agaagtacaa actcattgac accg 24 <210> 745 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 745 cgtaccatca ttcattgatt catgcg 26 <746> 746 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 746 cgtaccatca ttcattgatt catgca 26 <210> 747 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 747 tggacaatgg tgacccgatg t 21 <210> 748 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 748 ccttatgcaa caactcagcg tac 23 <210> 749 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 749 ttttgtctgg tccatttttg ctgt 24 <750> 750 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 750 ttgtctggtc catttttgct gc 22 <210> 751 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 751 atcccctgac tccacgcttt 20 <210> 752 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 752 tggtataaat gttacccaat ttcggttt 28 <210> 753 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 753 aggtttggag gagcagaaca tatc 24 <210> 754 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 754 aggtttggag gagcagaaca tatt 24 <210> 755 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 755 tcaagttctg ctcatgtaat tgaatctcat ca 32 <210> 756 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 756 tttctagttt ctgctcattt tcaaggt 27 <210> 757 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 757 tgaaccttct tatgctcagc gtaag 25 <210> 758 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 758 tgaaccttct tatgctcagc gtaaa 25 <210> 759 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 759 ggttccaaac aacaagcacc aaac 24 <210> 760 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 760 agtgaataat catgcttttg aaccttct 28 <210> 761 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 761 ggaatttctt gtgctcatcg ct 22 <210> 762 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 762 ggaatttctt gtgctcatcg cc 22 <210> 763 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 763 tggcagaatc gtaataatct atttaacgag gg 32 <210> 764 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 764 atggtgggcc gcagg 15 <210> 765 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 765 cagcaacttg agggaagcg 19 <210> 766 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 766 tcagcaactt gagggaagca 20 <210> 767 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 767 ctccttttcc acctctaact agacgg 26 <210> 768 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 768 cactgaaaga actagaacac atggt 25

Claims (5)

서열번호 1 내지 768로 표시된 올리고뉴클레오티드에서 인접한 4개의 올리고뉴클레오티드가 하나의 프라이머 세트인 것을 특징으로 하는 192개의 올리고뉴클레오티드 프라이머 세트를 포함하는, 자포니카(Oryza sativa L. subsp. japonica K.) 및 인디카(O. sativa L. subsp. indica K.) 벼의 감별용 프라이머 세트.Japonica ( Oryza sativa L. subsp . Japonica K.) and Indica, comprising 192 oligonucleotide primer sets, characterized in that four adjacent oligonucleotides in the oligonucleotides represented by SEQ ID NOS: 1-768 are one primer set. O. sativa L. subsp.indica K.) A set of primers for discriminating rice. 삭제delete 제1항의 프라이머 세트; 및 증폭 반응을 수행하기 위한 시약을 포함하는 자포니카(Oryza sativa L. subsp. japonica K.) 및 인디카(O. sativa L. subsp. indica K.) 벼의 감별용 키트.A primer set of claim 1; And a kit for discriminating japonica ( Oryza sativa L. subsp . Japonica K.) and indica ( O. sativa L. subsp . Indica K.) rice comprising a reagent for carrying out an amplification reaction. 제3항에 있어서, 상기 증폭 반응을 수행하기 위한 시약은 DNA 폴리머라제, dNTPs 및 버퍼를 포함하는 것을 특징으로 하는 자포니카 및 인디카 벼의 감별용 키트.The kit of claim 3, wherein the reagent for performing the amplification reaction comprises DNA polymerase, dNTPs and a buffer. 벼 시료에서 게놈 DNA를 분리하는 단계;
상기 분리된 게놈 DNA를 주형으로 하고, 제1항의 프라이머 세트를 이용하여 증폭 반응을 수행하여 표적 서열을 증폭하는 단계; 및
상기 증폭 단계의 산물의 유전자형을 결정하는 단계;를 포함하는, 자포니카(Oryza sativa L. subsp. japonica K.) 및 인디카(O. sativa L. subsp. indica K.) 벼를 감별하는 방법.Separating genomic DNA from the rice sample;
Amplifying a target sequence by using the isolated genomic DNA as a template and performing an amplification reaction using the primer set of claim 1; And
Determining the genotype of the product of the amplification step; comprising, japonica ( Oryza sativa L. subsp . Japonica K.) and indica ( O. sativa L. subsp . Indica K.).
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JP2006174714A (en) * 2004-12-21 2006-07-06 Plant Genome Center Co Ltd Genome polymorphism marker of oryza sativa and use thereof

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JP2006174714A (en) * 2004-12-21 2006-07-06 Plant Genome Center Co Ltd Genome polymorphism marker of oryza sativa and use thereof

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Thomson et al, Plant Breed. Biotech. 2014, 2(3):195-212

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