KR102055148B1 - Aquatic plant culture system and aquatic plant culture method using the same - Google Patents

Abstract

The present invention relates to a myelin cultivation system and a method for cultivating a myelin sheath using the same, the cultivation of the mycelium in the culture medium without pesticides simple structure, the present invention is provided with an accommodating space therein and the accommodating space on the top It is provided with a communication opening 102, transparent culture vessel 104 made of glass or synthetic resin material; A culture solution 106 positioned at a predetermined height on an inner bottom upper surface of the culture vessel 104 to provide nutrition to a few seconds; Sealing the opening 102 of the culture vessel 104, but having a first through hole 108 in communication with the receiving space of the culture vessel 104 on one side of the inside and from the first through hole 108 A stopper 112 made of a silicone material having a second through hole 110 communicated with a receiving space of the culture vessel 104 at a predetermined interval at an inner side thereof; A first injection pipe 114 inserted into the first through hole 108 of the stopper 112 and having one end spaced from the culture solution 106 at a predetermined interval; A connection hose 116 made of a silicon material connected to one end of the first injection pipe 114 and positioned in the culture solution 106; A second injection pipe 118 connected to an end of the first injection pipe 114; A third injection pipe 120 positioned at a predetermined interval from the second injection pipe 118; It is connected to the third injection pipe 120, and includes an air injection pump 122, such as to inhale the outside air to inject the sucked air into the third injection pipe (120).

Description

Aquatic plant culture system and aquatic plant culture method using the same

The present invention relates to a myelin cultivation system, and more particularly, to a myelin cultivation system and a method of cultivating a myelin cultivation using the same, which is simple in structure but cultivated aseptically without pesticides in a culture medium.

Plants (水草) means grass that grows in or near water. Compared with the land plants, the aquatic plants have a unique appearance such as roots, roots, leaves, and flowers.

However, due to the property of growing in the water or in the water, the water plants need a large amount of water in the growing environment, requires the administration of a fertilizer composition different from the land plants, there is a problem that is vulnerable to pests, to grow the plants For this purpose, very delicate management is required in terms of water change and fertilizer composition addition.

Therefore, there is an urgent need for a method of producing clean plants without sterilization so that anyone can easily grow plants.

Looking at the patent literature indirectly related to the myelin culture structure, KR Patent Application No. 10-2000-0067450 (name of the invention: sewage purification system for sewage purification), the main raw material is produced by recycling waste plastic, contained in the sewage Separators and sediments for segregation and sedimentation apparatuses for sedimentation of sand and gravel (80) and sedimentation apparatuses (120) for sedimentation of soil or heavy metal residues not precipitated in aggregate sedimentation apparatus (88) Purifier to purify the debris from entering the incubator, and cultivate plants and purify sewage using plants. Disclosed is a water purification system for sewage purification, including a water culture system for discharging clean water downstream, and KR Patent Application No. 10-2000-0064074 (Plastic water culture apparatus for waste water purification) discloses a water-based culture apparatus (10). ) And a buoyancy type myelin culture device (40) capable of curing a large number of plants on the water and a fixed device 20 to fix the myelin sheath culture device (10) on the waterway, and also a buoyancy type myelin culture device (40) To lose or destroy due to the resistance of water

Disclosed is a wastewater purification apparatus for treating wastewater comprising a fixing device 100 to prevent and a moving device 103 which allows the myelin culture device 40 to move in accordance with the change of the water level, KR patent application No. 10- In 2002-0038556 (name of the invention: the natural purification system of sewage or wastewater using four seasons without greenhouses), the treated water or waste water which has undergone basic pretreatment such as biological treatment or sedimentation method in sewage, wastewater, sewage, salt wastewater, etc. A distribution tank 10 for directly receiving contaminated river water and evenly transferring the contaminated river water to the lower part of the aquatic plant tank; A distribution layer 51, a support layer 52, a sacrificial layer 53 and a filtration layer 54 are provided from the lower part to the upper part, and the myelin sheath 20 is planted in the filtration layer, and the treated water which flowed in from the said distribution tank was sequentially A plurality of aquatic plant tanks for upflow filtration; And a main drain pipe 11 including a lower drain pipe 111 and an upper drain pipe 112 to remove foreign substances in the lower and upper portions of the aquatic plant tank; sewage or wastewater using four seasons The natural purification system of this is disclosed.

However, there is no disclosure of a patent document about a myelin culture structure or a myelin culture composition that can cultivate sterile clean plants in large quantities.

In order to solve the above problem, it was filed in the Patent Office with the patent application No. 10-2014-0105726 dated August 14, 2014 (name of the invention: myelin culture structure), the claims "100 parts by weight of soil composition, the Based on 100 parts by weight of the soil composition of Coco pit and spagnum moss in a ratio of 7: 3 by weight of the filler prepared by mixing 30-120 parts by weight and 10-50 parts by weight of the fertilizer composition, the soil The composition comprises cultured soil and loess in a ratio of 1: 1 by weight, and the fertilizer composition is prepared by mixing the compost composition and EM solution in a ratio of 5: 1 by weight and then aging.

The aging temperature is 5-40 ℃, the maturation period is 30-100 days, the fertilizer composition is 1 to 1 parts by weight of tourmaline powder and limestone powder based on 100 parts by weight of the fertilizer composition 1: 1 to 1 to An inorganic fertilizer further comprises a ratio of 1: 10, wherein the inorganic fertilizer is a composition for cultivating myelin (水草) containing additional 0.1-1 parts by weight of magnesium carbonate (MgCO3) based on 100 parts by weight of the inorganic fertilizer. " to be.

However, the conventional myelin sheath culture structure has a problem in that not only the structure is complicated but also the myelin can be cultured aseptically.

Accordingly, the present invention has been made in order to solve the above-mentioned problems according to the prior art, the object of the present invention is a simple myelin culture system for cultivating the myelin without a pesticide in the culture medium in the water while the structure is simple and To provide a used myelin culture method.

However, the object of the present invention is not limited to the above-mentioned object, other objects that are not mentioned will be clearly understood by those skilled in the art from the following description.

Myelin sheath culture system according to the present invention for achieving the above object,

A transparent culture vessel 104 having an accommodation space therein and having an opening 102 communicating with the accommodation space at the top thereof, wherein the transparent culture vessel is made of glass or synthetic resin material;

A culture solution 106 positioned at a predetermined height on an inner bottom upper surface of the culture vessel 104 to provide nutrition to a few seconds;

Sealing the opening 102 of the culture vessel 104, but having a first through hole 108 in communication with the receiving space of the culture vessel 104 on one side of the inside and from the first through hole 108 A stopper 112 made of a silicone material having a second through hole 110 communicated with a receiving space of the culture vessel 104 at a predetermined interval at an inner side thereof;

A first injection pipe 114 inserted into the first through hole 108 of the stopper 112 and having one end spaced from the culture solution 106 at a predetermined interval;

A connection hose 116 made of a silicon material connected to one end of the first injection pipe 114 and positioned in the culture solution 106;

A second injection pipe 118 connected to an end of the first injection pipe 114;

A third injection pipe 120 positioned at a predetermined interval from the second injection pipe 118;

An air injection pump 122 connected to the third injection pipe 120 and injecting external air to suck the outside air into the third injection pipe 120;

It is installed between the end of the second injection pipe 118 and the end of the third injection pipe 120, filtering the bacteria from the outside air injected from the third injection pipe 120 and the second injection of the filtered outside air It includes a first filter 124 to be injected into the tube 118, the first injection tube 114, the connection hose 116.

And, myelin culture method using the myelin culture system according to the present invention for achieving the above object,

30 seconds by weight of water and ethanol: 70 parts by weight of the first mixture is mixed in about 10 to 60 seconds after the first sterilization treatment, the first sterilized water and 98 parts by weight of sodium hypochlorite: A pre-culture step of sterilization by adding about 10 to 60 seconds to the second mixture mixed in 2 parts by weight;

After culturing the pre-cultivation step, cutting the stem nodes of the myelin and aseptic body securing step of cultivating the myelin in a mixture consisting of MS (murashige & skoog medium) medium containing inorganic nutrients and vitamins, carbon source, water;

After the aseptic body securing step, after opening the stopper 112 is inserted into the opening 102 of the culture vessel 104, MS (murashige & skoog medium) containing the inorganic nutrients and vitamins, carbon source, water A myelin sheath step of introducing the myelin cultivated in the mixture into the culture solution 106 accommodated in the accommodation space of the culture vessel 104 through the opening 102;

After the water input step, the air injection pump 122 is driven to filter the bacteria in the outside air with the first filter and inject the filtered outside air into the culture vessel 104 to better melt the air in the culture solution. In addition, by driving the LED irradiation unit 134 to irradiate the light to the culture vessel 104 to cause the myelin sheath photosynthesis comprises a proliferation culture step of propagating the myelin sheath in the culture vessel (104).

As described above, the myelin culture system and the myelin culture method using the same according to the present invention have a simple structure and have the effect of culturing a large number of myelin in a short period of time.

In particular, the present invention has the effect that can be cultured aseptic state without agrochemicals in the culture medium.

In addition, the present invention not only has the effect of efficiently cultivating a large number of plants in a narrow space, but also has the effect of being able to be produced without being influenced by the season by being cultured indoors.

In addition, the present invention is faster than the growth rate of 8 to 10 times more than the conventional myelin culture system, it is possible to produce a healthy myelin without the use of pesticides without aquatic plants, produced in a sterile hermetic environment, bacteria, snails It is effective in preventing external pollutants such as parasites and parasites.

In addition, the present invention is not only effective in the import and export of aseptic culture for plant resources that were difficult to import and export due to bacteria in a few seconds, as well as the effect of preservation and propagation of rare aquatic plant resources such as endangered species. have.

1 is a view showing a myelin sheath culture system according to the present invention,
2 to 3 is an enlarged view of the main part of the myelin sheath culture system of FIG.
Figure 4 is an exploded view of the main part of the myelin sheath culture system of Figure 1,
5 is a view schematically showing a state of use of the myelin sheath culture system according to the present invention,
6 is a view showing a myelin photograph before incubation in the myelin culture system according to the present invention,
7 is a view showing a picture of the myelin sheath cultured in the myelin sheath culture system according to the present invention.

Hereinafter, a preferred embodiment of the myelin sheath culture system and the myelin sheath culture method using the same according to the present invention.

In the following description of the present invention, detailed descriptions of related known functions or configurations will be omitted when it is determined that the detailed description of the present invention may unnecessarily obscure the subject matter of the present invention.

1 is a view showing the myelin sheath culture system according to the present invention, Figures 2 to 3 is an enlarged view of the main part of the myelin sheath culture system of Figure 1, Figure 4 is a principal decomposition of the myelin sheath culture system of Figure 1.

As shown in Figure 1 to 4 myelin sheath culture system 100 according to the present invention,

A transparent culture vessel 104 having an accommodation space therein and having an opening 102 communicating with the accommodation space at the top thereof, wherein the transparent culture vessel is made of glass or synthetic resin material;

A culture solution 106 positioned at a predetermined height on an inner bottom upper surface of the culture vessel 104 to provide nutrition to a few seconds;

Sealing the opening 102 of the culture vessel 104, but having a first through hole 108 in communication with the receiving space of the culture vessel 104 on one side of the inside and from the first through hole 108 A stopper 112 made of a silicone material having a second through hole 110 communicated with a receiving space of the culture vessel 104 at a predetermined interval at an inner side thereof;

A first injection pipe 114 inserted into the first through hole 108 of the stopper 112 and having one end spaced from the culture solution 106 at a predetermined interval;

A connection hose 116 made of a silicon material connected to one end of the first injection pipe 114 and positioned in the culture solution 106;

A second injection pipe 118 connected to an end of the first injection pipe 114;

A third injection pipe 120 positioned at a predetermined interval from the second injection pipe 118;

An air injection pump 122 connected to the third injection pipe 120 and injecting external air to suck the outside air into the third injection pipe 120;

It is installed between the end of the second injection pipe 118 and the end of the third injection pipe 120, filtering the bacteria from the outside air injected from the third injection pipe 120 and the second injection of the filtered outside air A first filter 124 injected into the pipe 118, the first injection pipe 114, and the connection hose 116;

The air stone is connected to the end of the connection hose 116 to convert the filtered outside air into fine air to inject the fine air into the culture medium 106 and at the same time circulating the culture solution to make the air dissolved in the culture medium better ( 126);

Is inserted into the second through-hole 110 of the stopper 112, one end from the culture solution 106 is positioned at a predetermined interval to discharge the internal air generated in the culture vessel 104 to the second discharge pipe One discharge pipe 128;

A second discharge pipe 130 connected to an end of the first discharge pipe 128 to discharge internal air discharged from the first discharge pipe 128 to a second filter;

A second filter 132 installed at an end of the second discharge pipe 130 to filter bacteria from the internal air discharged from the second discharge pipe 130 and to discharge the filtered internal air to the outside;

The LED irradiator 134 is provided with an LED (not shown) supporting the culture vessel 104 and irradiating light with the culture solution accommodated in the culture vessel so that plants can photosynthesize.

And, the myelin sheath culture system 100 according to the present invention,

It further includes a control unit (not shown) for controlling the myelin culture system according to a predetermined program,

The control unit is preferably provided on one side of the LED irradiation unit 134.

Here, the air injection pump 122 and the LED irradiation unit 134 is electrically connected to the control unit is controlled by the control signal of the control unit.

On the other hand, the culture solution 106,

MS (murashige & skoog medium) containing inorganic nutrients and vitamins, carbon sources, water, plant growth regulators are mixed.

Referring to the assembly process of the myelin sheath culture system according to the present invention configured as described above are as follows.

Here, the assembly process of the myelin sheath culture system according to the present invention can be changed as much as the assembler.

First, the culture vessel 104 having an accommodation space therein and having an opening 102 in communication with the accommodation space at the top.

Here, the culture vessel 104 is preferably made of transparent glass or transparent synthetic resin material.

In addition, MS (murashige & skoog medium) medium containing carbon and inorganic nutrients and vitamins that provide nutrition in a few seconds to the opening space of the culture vessel 104 to a predetermined height in the receiving space, carbon, water, plant growth regulator Pour the mixed culture solution 106.

In addition, the stopper 112 having the first through hole 108 on the inner side and the second through hole 110 on the inner side with a predetermined distance from the first through hole 108 is positioned. . Here, the stopper 112 is preferably made of a silicon material.

Then, the first injection pipe 114 is forcibly fitted into the first through hole 108 of the stopper 112, and then the connection hose 116 is connected to one end of the first injection pipe 114. Here, the connection hose 116 is preferably made of a silicon material.

Then, the fine air is injected into the culture medium 106 at the end of the connection hose 116 and the air stone 126 is connected to circulate the culture solution so that the air is better dissolved in the culture solution.

Then, the second injection pipe 118 is connected to the end of the first injection pipe 114.

Then, the first discharge pipe 128 is forcibly fitted into the second through hole 110 of the stopper 112, and then the second discharge pipe 130 is connected to the end of the first discharge pipe 128.

Then, the stopper 112 is forcibly inserted into the opening 102 of the culture vessel 104, and then the third injection pipe 120 is positioned at a predetermined interval from the second injection pipe 118.

Then, after positioning the air injection pump 122, the third injection pipe 120 is connected to one side of the air injection pump 122.

Then, after placing the first filter 124 for filtering the bacteria, the first filter 124 is installed between the end of the second injection pipe 118 and the end of the third injection pipe 120.

Then, after placing the second filter 132 for filtering the bacteria, the second filter 132 is installed at the end of the second discharge pipe (130).

In addition, after positioning the LED irradiation unit 134 in which the control unit (not shown) is installed, and placing the culture vessel 104 on the upper surface of the LED irradiation unit 134, the air injection pump 122 in the control unit. ) And the LED irradiation unit 134 electrically. Here, the LED irradiation unit 134 has a plurality of LEDs (not shown).

Looking at the myelin sheath culture method using the myelin sheath culture system assembled as follows.

First, the myelin sheath is added to the first mixture of 30 parts by weight and 70 parts by weight of ethanol for about 30 seconds, and the first sterilization treatment is carried out.

Then, the first sterilized myelin is added to a second mixture of water and sodium hypochlorite 98 parts by weight: 2 parts by weight for about 30 seconds, the second sterilization treatment is pulled out and treated before incubation.

Then, after cutting the stem node of the pre-treated myelin sheath, as shown in Figure 6, the myelin is incubated in a mixture consisting of MS (murashige & skoog medium) medium containing inorganic nutrients and vitamins, carbon source, water.

Then, after opening the stopper 112, myelin cultivated in a mixture consisting of the MS (murashige & skoog medium) medium containing the inorganic nutrients and vitamins, carbon source, water through the opening 102, as shown in FIG. Into the culture solution 106 contained in the receiving space of the culture vessel 104. In this case, the culture solution 106 is a mixture of MS (murashige & skoog medium) medium containing inorganic nutrients and vitamins, carbon source, water, plant growth regulator.

After inserting the stopper 112 into the opening 102 of the culture vessel 104, the air injection pump 122 and the LED irradiation unit 134 are driven.

And, according to the driving of the air injection pump 122,

The air injection pump 122 sucks outside air and injects the sucked outside air into the first filter 124 through the third injection pipe 120.

In addition, the first filter 124 filters the bacteria from the injected external air and transfers the filtered external air to the second injection pipe 118, the first injection pipe 114, the connection hose 116, and the air stone 126. )

The air stone 126 converts the filtered outside air into fine air, injects fine air into the culture solution 106, and simultaneously circulates the culture solution to proliferate and culture plants. Here, the reason for circulating the culture is to allow the air to melt better in the culture.

On the other hand, according to the driving of the LED irradiation unit 134,

The LED (not shown) installed in the LED irradiator 134 irradiates light to allow photosynthesis with the culture solution accommodated in the culture vessel 104.

Therefore, the LED irradiation unit 134 propagates and cultures the myelin sheath to enable the myelin sheath photosynthesis.

On the other hand, the first discharge pipe 128 discharges the internal air generated in the culture vessel 104 to the second discharge pipe,

The second discharge pipe 130 discharges the internal air discharged from the first discharge pipe 128 to the second filter.

The second filter 132 filters bacteria from the internal air discharged from the second discharge pipe 130 and discharges the filtered internal air to the outside.

The detailed description of the invention is merely exemplary of the invention, which is used only for the purpose of illustrating the invention and is not intended to limit the scope of the invention as defined in the meaning or claims. Therefore, those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.

100: water plant culture system 102: opening
104: culture vessel 106: culture solution
108: first through hole 110: second through hole
112: stopper 114: first injection tube
116: connecting hose 118: second injection pipe
120: third injection pipe 122: air injection pump
124: first filter 126: air stone
128: first discharge pipe 130: second discharge pipe
132: second filter 134: LED irradiation unit

Claims (5)

A transparent culture vessel 104 having an accommodation space therein and having an opening 102 communicating with the accommodation space at the top thereof, wherein the transparent culture vessel is made of glass or synthetic resin material; A culture solution 106 positioned at a predetermined height on an inner bottom upper surface of the culture vessel 104 to provide nutrition to a few seconds; Sealing the opening 102 of the culture vessel 104, but having a first through hole 108 in communication with the receiving space of the culture vessel 104 on one side of the inside and from the first through hole 108 A stopper 112 made of a silicone material having a second through hole 110 communicated with a receiving space of the culture vessel 104 at a predetermined interval at an inner side thereof; A first injection pipe 114 inserted into the first through hole 108 of the stopper 112 and having one end spaced from the culture solution 106 at a predetermined interval; A connection hose 116 made of a silicon material connected to one end of the first injection pipe 114 and positioned in the culture solution 106; A second injection pipe 118 connected to an end of the first injection pipe 114; A third injection pipe 120 positioned at a predetermined interval from the second injection pipe 118; In the myelin cultivation system connected to the third injection pipe 120, including an air injection pump 122 for sucking the outside air and injecting the sucked air into the third injection pipe 120,
It is installed between the end of the second injection pipe 118 and the end of the third injection pipe 120, filtering the bacteria from the outside air injected from the third injection pipe 120 and the second injection of the filtered outside air A first filter 124 injected into the pipe 118, the first injection pipe 114, and the connection hose 116;
An air stone which is connected to the end of the connection hose 116 and converts the filtered outside air into fine air to inject the fine air into the culture medium 106 and circulates the culture solution to better melt the air in the culture solution ( 126);
Is inserted into the second through-hole 110 of the stopper 112, the one end is located at a predetermined interval from the culture solution 106 to discharge the internal air generated in the culture vessel 104 to the second discharge pipe One discharge pipe 128;
A second discharge pipe 130 connected to an end of the first discharge pipe 128 to discharge internal air discharged from the first discharge pipe 128 to a second filter;
A second filter 132 installed at an end of the second discharge pipe 130 to filter bacteria from the internal air discharged from the second discharge pipe 130 and to discharge the filtered internal air to the outside;
Further comprising an LED irradiation unit 134 supporting the culture vessel 104 and having an LED for irradiating light to the myelin sheath photosynthesis into the culture solution contained in the culture vessel,
Here, the culture solution 106, the myelin culture system, characterized in that the mixture of MS (murashige & skoog medium) medium containing inorganic nutrients and vitamins, carbon source, water, plant growth regulator.
30 seconds by weight of water and ethanol: 70 parts by weight of the first mixture was mixed in about 30 seconds after the first sterilization, water and sodium hypochlorite 98 parts by weight: 2 weight A pretreatment treatment step of adding a second mixture to the second mixture by mixing for about 30 seconds to sterilize the second mixture;
After culturing the pre-cultivation step, the stem section of the myelin sheath cutting and sterile agent for culturing the myelin in a mixture consisting of MS (murashige & skoog medium) medium containing inorganic nutrients and vitamins, carbon source, water;
After the aseptic agent securing step, the stopper 112 is opened and the myelin sheath incubated in a mixture consisting of MS (murashige & skoog medium) medium containing inorganic nutrients and vitamins, a carbon source, and water of the culture vessel 104 through the opening 102. Injected into the culture solution 106 accommodated in the receiving space, the culture solution 106 is a myelin sheath that is mixed with MS (murashige & skoog medium) medium containing inorganic nutrients and vitamins, carbon source, water, plant growth regulator;
After the water input step, the air injection pump 122 is driven to filter the bacteria in the outside air with the first filter and inject the filtered outside air into the culture vessel 104 to better melt the air in the culture solution. In addition, by driving the LED irradiation unit 134 and irradiated with light to the culture vessel 104 so that the myelin occurs photosynthesis, the myelin sheath comprising a proliferation culture step of propagating the myelin sheath in the culture vessel 104 Weed Cultivation Method Using Culture System

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