KR102053222B1 - Pet food composition comprising onion peel - Google Patents

Abstract

The present invention relates to a pet food composition comprising onion peel. The composition of the present invention can regulate blood lipid levels through the inhibition of lipase activity in the body, thereby having an excellent effect of improving obesity. In addition, the composition fundamentally improves inflammation through the inhibition of inflammation-related factors and causes side effects during administration by using a plant harmless to the living material as a raw material, thereby being safe.

Description

Pet food composition containing onion peel {PET FOOD COMPOSITION COMPRISING ONION PEEL}

Disclosed herein is a pet food composition comprising onion peel.

The pet market is growing steadily due to the aging of the population and the increase in the number of single-person households. Specifically, the domestic pet market has developed rapidly since the late 90s, and is currently reported to be worth 1 trillion won, and overseas markets such as the US, Japan, and Europe are currently worth about US $ 60 billion, with annual growth of 1.3%. Doing.

The strongest sector of the pet market is the pet food market. In particular, in the United States, dogs and cats that ate Chinese feeds died in 2007 due to acute renal failure. Doing. In addition, as the movement of pets decreases due to changes in human living spaces and changes in living patterns, pets are suffering from modern diseases such as obesity and arteriosclerosis as well as humans. have.

KR 10-2017-0143356 A

In one aspect, an object of the present invention is to provide a composition for companion food without side effects.

In one aspect, an object of the present invention is to prepare a food composition for pets, utilizing onion peel discarded without being used as a food material.

In one aspect, an object of the present invention is to provide a food composition for pets having an anti-inflammatory effect and having an anti-inflammatory effect.

In order to achieve the above object, the present invention provides an animal food composition comprising an onion extract, paprika extract and tomato extract in one aspect.

Animal food composition, which is an aspect of the present invention, can regulate blood lipid levels through the inhibition of lipase activity in the body, thereby improving obesity. In addition, the composition may fundamentally improve inflammation through inhibition of inflammation related factors. In addition, since the composition uses a plant harmless to the living material as a raw material, side effects do not occur during administration, so it is safe.

Hereinafter, the present invention will be described in detail.

In one aspect, the present invention is an animal food composition comprising onion extract, paprika extract and tomato extract.

The composition may include one or more of the following properties:

i) anti-inflammatory;

ii) lowering cholesterol in the blood; And

iii) improving obesity.

In one aspect, the onion may include an onion peel.

In the present specification, the "extract" may include not only crude extract, but also fractions obtained by fractionating the crude extract. That is, the extract includes not only those obtained by using the above-described extraction solvent, but also those obtained by additionally applying a purification process. The extract of the present embodiment prepared as described above may be implemented in various forms such as solid and liquid.

In one aspect, the composition may further comprise black barley extract and Sinyi extract.

"Shinyi" may mean a bud of magnolia and plants.

In the same aspect as above, the composition may include onion extract, paprika extract, tomato extract, black barley extract and sinyi extract, in order, 1: 1 to 3: 5 to 7: 0.5 to 1: 0.5 to 1 It may be included in the weight ratio of. The composition, specifically, onion extract, paprika extract, tomato extract, black barley extract and Sinyi extract may include a weight ratio of 1: 1.8 ~ 2.2: 5.5 ~ 6.5: 0.6 ~ 0.8: 0.6 ~ 0.8.

When extracts in the composition are included in the weight ratio, the composition may exhibit the most excellent obesity improvement effect and anti-inflammatory effect.

In one aspect, the composition may inhibit the activity of lipase in vivo, thereby inhibiting the digestion and absorption of fat in the body, thereby improving the excessive absorption and accumulation of fat or lipids in the body.

In one aspect, the lipid may comprise cholesterol.

In one aspect, the composition may be a composition for preventing or improving various cardiovascular diseases. For example, it can be used as a composition for preventing or improving hyperlipidemia, angina pectoris, myocardial infarction, arteriosclerosis, ischemic stroke and the like.

In addition, the composition can inhibit the production of inflammation-related factors. Specifically, by inhibiting the production or secretion of inkeryukin-8 (IL-8), it is possible to fundamentally inhibit or improve inflammation.

In one aspect, the extraction solvent of the extract may include water, an organic solvent or an organic solvent aqueous solution.

The organic solvent may be hexane, methylene chloride, alcohol, or the like as the organic solvent, but is not limited thereto. In the above, water includes distilled water or purified water, and the organic solvent is a group consisting of alcohol, acetone, ether, ethyl acetate, diethyl ether, methanol, ethyl methyl ketone, and chloroform, for example, a lower alcohol having ₁ to ₅ carbon atoms. It may include one or more selected from.

When the extraction solvent is water, heat extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction may be used, and preferably may be warm extraction, preferably about 40 to 120 ℃, more preferably about Extraction may be performed at a temperature of 70 ° C., but is not limited thereto. Extraction time may preferably be performed for about 1 to 4 hours, more preferably about 2 hours, but is not limited thereto.

Preferably, the extraction solvent of the onion extract, paprika extract, and wasabi extract may be an alcohol, more preferably, the alcohol may be ethanol.

Also, preferably, the ethanol may include an aqueous ethanol solution, and may include about 20-40% aqueous ethanol solution.

In addition, in one aspect, the extraction solvent of the black barley extract and Sinyi extract, preferably, may be water.

In one aspect, the onion extract, paprika extract, tomato extract, black barley extract and shinyi extract, based on the total weight of the composition, may be included in 0.1 to 99% by weight.

There is no particular limitation on the kind of food. Examples of the food to which the extract of the present invention may be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionary, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, teas, Drinks, alcoholic beverages, and vitamin complexes.

In addition to the above, the extract of the present invention contains various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonic acid. Carbonating agents and the like used in beverages.

The composition of the present invention may be prepared as a pharmaceutical composition in addition to the food composition. The pharmaceutical composition may be prepared according to conventional methods, and in the preparation of the dosage form, it is preferable to mix or dilute the active ingredient with the carrier or to enclose it in a carrier in the form of a container. When the carrier is used as a diluent, it may be a solid, semisolid or liquid substance which acts as a carrier, excipient or medium for the active ingredient. Thus, the formulation may be in the form of tablets, pills, powders, sachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, sterile injectables, sterile powders and the like.

Examples of suitable carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propyl Hydroxybenzoate, talc, magnesium stearate and mineral oil. The formulation may further comprise fillers, anti-coagulants, lubricants, wetting agents, fragrances, emulsifiers, preservatives and the like. Compositions of the present invention may be formulated using methods well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal.

The pharmaceutical composition of the present invention may be administered through several routes including oral, transdermal, subcutaneous, intravenous, abdominal, muscle, topical application, patch and iontophoresis, of which topical application and oral administration are preferred. Do.

Typical daily dosages of the compositions may range from 1 to 100 mg / kg body weight, preferably 5 to 70 mg / kg body weight, and may be administered once or in several portions. However, it should be understood that the actual dosage of the composition should be determined in light of several related factors such as the disease to be treated, the route of administration, age, sex and weight, and the severity of the disease, and thus the dosage may be It does not limit the scope of the invention.

In addition, in one aspect, the composition may be a food composition. The health food may be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, and the like, but is not limited thereto and may be manufactured and processed in any form according to the law.

The mixed amount of the active ingredient may be suitably determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, each of the extracts of the present invention is added in an amount of 15% by weight or less, preferably 10% by weight or less, based on the raw materials, in the manufacture of food or beverage. However, in the case of long-term intake for health and hygiene or health control purposes, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety.

In one aspect, the onion may comprise red onions, the paprika may comprise red paprika.

In addition, in the above aspect, the animal may include a companion animal, for example, may include a mammal such as a dog, a cat, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to the following Preparation Examples, Examples and Experimental Examples. However, these Examples and Experimental Examples are provided only for the purpose of illustration to help the understanding of the present invention is not limited to the scope and scope of the present invention by the following examples.

[Production Example] Preparation of Onion, Paprika, Tomato, Black Barley, Sour Extract

Preparation Example 1 Preparation of Ethanol Extract

Onion skin, paprika, tomato, black barley, and sour each dried and crushed 30g of powder obtained by grinding each into 600g of 30% ethanol and left for 2 hours at 70 ℃, filtered on a non-woven fabric having a hole of 6μm It was. The filtered solution was concentrated at 140 ° C. at 140 rpm, and the produced concentrate was prepared as a powder using a lyophilization method.

Preparation Example 2 Preparation of Hot Water Extract

After peeling onion skin, paprika, tomato, black barley and sour, each 30g of each powder obtained by grinding is put into 600g of purified water and left at 70 ℃ for 2 hours, and then filtered on a nonwoven fabric having a hole of 6μm. It was. The filtered solution was concentrated to 140 rpm at 65 ° C., and then each extract in powder form was prepared by lyophilization.

Preparation Example 3 Preparation of Composition

The extracts were mixed to prepare a composition in the ratios described in Table 1 below. The extracts described in Table 1 used ethanol as the extraction solvent.

Onion peel paprika tomato Black barley God Example 1 One 2 6 - - Example 2 One 2 6 0.7 0.7 Example 3 One 1.5 6 0.7 0.7 Example 4 One 2.5 6 0.7 0.7 Example 5 One 2 5 0.7 0.7 Example 6 One 2 7 0.7 0.7 Example 7 One 2 6 0.5 0.7 Example 8 One 2 6 One 0.7 Example 9 One 2 6 0.7 0.5 Example 10 One 2 6 0.7 One

Experimental Example 1 Confirming the Inhibitory Effect of Lipase Activity

Experimental Example 1-1 Effect of Extract Content

Lipase inhibitory activity was assessed using previously reported methods (Nakai, N. et al. Inhibitory effects of oolong tea polyphenols on pancreatic lipase in vitro. J. Agric. Food Chem. 53, 4593-4598 (2005). ). Enzyme solution was prepared by dissolving porcine pancreatic lipase (Sigma, St. Louis, MO) in 0.1M Tris-HCl buffer (pH 8).

The extracts were mixed in enzyme buffer to a concentration of 0.5 ug / mL at the compounding ratios described in Table 1 below, and 100 ul was left standing at 37 ° C. for 15 minutes. After standing, 10 uL of 10 mM para-nitrophenylbutyrate (p-NPB) was added and the enzyme reaction was performed at 37 ° C. for 15 minutes. Lipase activity was observed by measuring the hydrolysis of p-NPB to para-nitrophenol at 405 nm using a microplate reader.

The following equation was used to calculate activity inhibition.

Relative pancreatic lipase activity (%) =

 (Activity of compound in substrate-activity of negative control substance in the absence of substrate) / (activity of compound in substrate and activity of negative control substance in the absence of compound) Х 100.

Tertiary distilled water was used as a negative control. The lipase activity inhibition rate was calculated according to Equation 1 and the results are shown in Table 2.

As a result, it was found that the effect of the composition of Example 2 was the most excellent.

Relative pancreatic lipase activity (%) Example 1 100.2 ± 3.2 Example 2 82.1 ± 3.01 Example 3 95.1 ± 1.44 Example 4 89.2 ± 4.23 Example 5 93.1 ± 2.88 Example 6 88.4 ± 2.43 Example 7 94.1 ± 5.18 Example 8 87.1 ± 3.26 Example 9 94.7 ± 4.47 Example 10 86.9 ± 2.83

Experimental Example 1-2 Effect of Extract on Extraction Solvent

In order to examine whether the type of the extraction solvent used in the preparation of the extract affects the effect of the composition, by adjusting the extraction solvent of each extract as shown in Table 3, to prepare a composition and the experiment in the same manner as in Experiment 1-1 Was carried out. The content of the extract contained in the composition was mixed in the same ratio as in Example 2.

Onion peel paprika tomato Black barley God Example 11 water ethanol ethanol ethanol ethanol Example 12 ethanol water ethanol ethanol ethanol Example 13 ethanol ethanol water ethanol ethanol Example 14 ethanol ethanol ethanol water ethanol Example 15 ethanol ethanol ethanol ethanol water Example 16 water water ethanol ethanol ethanol Example 17 water ethanol water ethanol ethanol Example 18 water ethanol ethanol water ethanol Example 19 water ethanol ethanol ethanol water Example 20 ethanol water water ethanol ethanol Example 21 ethanol water ethanol water ethanol Example 22 ethanol water ethanol ethanol water Example 23 ethanol ethanol water water ethanol Example 24 ethanol ethanol water ethanol water Example 25 ethanol ethanol ethanol water water

As a result, as can be seen in Table 4, the onion peel, paprika and black barley were found to have the best lipase activity inhibitory effect, including those extracted with ethanol, tomatoes and sour water.

Relative pancreatic lipase activity (%) Example 11 79.1 ± 3.21 Example 12 80.7 ± 1.11 Example 13 78.7 ± 3.27 Example 14 78.3 ± 2.65 Example 15 78.9 ± 1.79 Example 16 77.7 ± 4.23 Example 17 79.1 ± 3.13 Example 18 78.0 ± 2.19 Example 19 78.5 ± 3.95 Example 20 78.0 ± 2.25 Example 21 77.9 ± 4.86 Example 22 78.8 ± 1.95 Example 23 79.3 ± 4.67 Example 24 73.1 ± 1.08 Example 25 79.3 ± 4.12

Experimental Example 2 Inflammation Control Effect

Experimental Example 2-1 Confirmation of Effect According to Extract Content

One day before the experiment, normal human skin keratinocytes (NHEK, obtained from Lonza) were dispensed into 96 well plates at 5x10 ⁴ cells / well, and then in a 37 ° C., 5% CO ₂ incubator. Incubated for 24 hours. After 24 hours, the cells were washed twice with PBS and changed to serum-free keratinocyte basement media (KBM). In each well, the composition mixed at the compounding ratio shown in Table 1 was treated at a concentration of 500 ppm for 30 minutes, and then treated with 1 ppm of LPS (lypopolysaccaride), a substance known as a major cause of inflammation.

Then, after incubation for 24 hours at 37 ℃, 5% CO ₂ incubator, the culture medium was taken and subjected to ELISA for Interleukin-8 (Interleukin-8, IL-8), the results are shown in Table 5 below. ELISA used the experimental method of the manufacturer (BD science).

As a result, the treatment of the composition of Example 2 was able to confirm the best IL-8 secretion control effect.

IL-8 secretion (pg / ml) Untreated control 912 LPS treatment group 7005.13 Example 1 3005.12 Example 2 1152.33 Example 3 2103.56 Example 4 1678.92 Example 5 2278.3 Example 6 1730.30 Example 7 2158.25 Example 8 1711.67 Example 9 2105.22 Example 10 1645.2

[Experimental Example 2-2] Confirm the effect of the extract solvent

In order to examine whether the type of the extraction solvent used in the preparation of the extract affects the effect of the composition, by adjusting the extraction solvent of each extract as shown in Table 3, the composition was prepared and the experiment was carried out as in Experimental Example 2-1. Was carried out. The content of the extract contained in the composition was mixed in the same ratio as in Example 2.

As a result, onion peel, paprika and black barley were found to have the best inhibitory effect of IL-8 secretion, including those extracted with ethanol, tomatoes and sour water.

IL-8 secretion (pg / ml) Untreated control 912 LPS treatment group 7005.13 Example 11 1009.3 Example 12 1080.21 Example 13 1082.33 Example 14 1079.36 Example 15 1100.08 Example 16 1058.92 Example 17 1103 Example 18 1075.24 Example 19 1066.33 Example 20 1089.33 Example 21 1101.82 Example 22 1065.33 Example 23 1088.17 Example 24 926.58 Example 25 998.23

Claims (7)

Regarding a composition comprising onion extract, paprika extract, tomato extract, black barley extract and shinyi extract,
The onion extract, paprika extract, tomato extract, black barley extract and shinyi extract are included in a weight ratio of 1: 1-3: 5-7: 0.5-1: 0.5-1,
The composition comprises an animal food composition comprising one or more of the following properties:
i) anti-inflammatory;
ii) reducing blood cholesterol; And
iii) improving obesity. delete delete The method of claim 1,
Extraction solvent of the extract, animal food composition comprising water, an organic solvent or an organic solvent aqueous solution. The method of claim 1,
Extraction solvent of the onion extract, paprika extract, and black barley extract includes ethanol,
The extraction solvent of the tomato extract and god shin extract comprises water, animal food composition. The method of claim 1,
The onion, the animal food composition comprising an onion peel. The method of claim 1,
The animal, a pet food composition comprising a pet.