KR102047338B1 - Apparatus for sterilizing microorganism using photoenergy and Sterilizing method using the same - Google Patents

Abstract

The present invention relates to a microbial disinfection device and a disinfection method, the disinfection device and disinfection method can safely remove microorganisms in food without deterioration of food quality and residues of chemicals, and the disinfection method is simple to simplify the process and There is a cost saving effect.

Description

Apparatus for sterilizing microorganism using photoenergy and Sterilizing method using the same}

The present invention relates to an apparatus for disinfecting microorganisms using light energy and a method for disinfecting agricultural products using the same.

In the recent well-being era, the consumption of fresh vegetables and fruits including sprout vegetables and baby vegetables is increasing rapidly. However, with the growth of the market related to fresh vegetables, the number of patients with food poisoning is increasing rapidly. Therefore, there is a need for an excellent food sterilization technique for preventing food poisoning.

In general, fresh agricultural products are shipped through screening, sterilization, washing, rinsing, dehydration, weighing and packaging. A typical process directly connected to the stability of fresh agricultural products is the sterilization and washing process. The most important purpose of the sterilization and washing process is to effectively remove microorganisms from raw materials such as agricultural products. In the case of lettuce, which is the main raw material of a salad that is conventionally distributed in a pack form, it shows a total bacterial count of about 10 ⁶ / g to 10 ⁹ / g, and it is impossible to remove 100% of these by a processing without heat treatment.

The conventional washing process is divided several times (1st to 3rd washing) and sterilization using sodium hypochlorite is performed at the same time. When washing is divided into 3 times, it is recommended to lower the number of microorganisms to 10 ⁵ / g immediately after manufacture. In case of using sodium hypochlorite, high concentration is required for a long time in order to obtain a bactericidal effect as low as 10 ³ / g.

However, it is difficult to obtain a stable sterilization effect in the actual processing step in which a large amount of raw materials are continuously washed, such that a large difference in sterilization effect occurs due to the severe variation in pH of the treatment liquid due to organic matter.

In addition, sodium hypochlorite is an inexpensive and effective sterilization method, but an attempt is being made to replace it due to the risk of residue and human safety.

Therefore, it is required to introduce a new microorganism removal technology with improved efficiency in terms of securing safety and cost through effective sterilization.

An object of the present invention relates to a device for disinfecting harmful microorganisms of food and a method for disinfecting agricultural products using the same, without reducing the freshness of food, deterioration of quality, loss of nutrition, resistance of microorganisms and risk of chemical residues. It is to provide a disinfection device for disinfecting harmful microorganisms.

The present invention to solve the above problems,

A container containing water;

A water inlet line formed at one side of the container and supplying water to be sterilized;

Water discharge line is formed on another side of the container, for discharging the sterilized water;

A partition wall formed inside the container to control the flow path of the water;

A photofunctional bead located between the partition walls in the container; And

Provided is a disinfection device including a light source device for supplying light spaced a distance from the container.

Disinfection apparatus and disinfection method according to the present invention can safely remove microorganisms in food without deterioration of food quality and residual chemicals, the disinfection method is simple, there is an effect of simplification and cost reduction.

1 is a schematic diagram of a disinfecting apparatus according to the present invention.
Figure 2 is a photograph of the disinfection device according to the present invention.
Figure 3 is a graph showing the disinfection efficacy of optical functional beads according to the conditions of Example 1, Comparative Example 1-1 and Comparative Example 1-2.
Figure 4 is a graph showing the disinfection efficacy of optical functional beads according to the conditions of Example 2, Comparative Example 2-1 and Comparative Example 2-2.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated more concretely.

The present invention relates to an apparatus for disinfecting microorganisms using light energy and a method for disinfecting agricultural products using the same.

Conventional microbial disinfection devices and methods of disinfection are complicated due to the need to perform several times of washing, and there is a risk of increase in costs. There was a problem.

The present invention has been made to solve the above problems, the microbial disinfection device using the light energy and the disinfection method using the same according to the present invention includes a simple process by including an optical functional bead to perform the antibacterial action by the light source supplied and Even at low cost, the number of microorganisms can be significantly reduced, effectively killing agricultural products.

Such, the disinfection device according to the present invention includes a container 10 for receiving water; A water inlet line formed at one side of the container 10 and supplying water to be sterilized; Is formed on another side of the container 10, the water outflow line for discharging the sterilized water; A partition wall formed inside the container 10 to control a flow path of water; Photo-functional beads positioned between the partition walls in the vessel 10; And a light source device spaced apart from the container 10 by a predetermined distance to supply light.

Figure 1 schematically shows the configuration of the disinfection apparatus according to the present invention, the disinfection apparatus according to the present invention is a container 10, water inlet line 20, water outlet line 30, partition 40, optical functionality And a bead 50 and a light source device 60.

As an example, the size of the container 10 may be about 130 mm in width (A), about 100 mm in length (B), and about 20 mm in height (C), but is not limited thereto.

The container 10 may have a structure in which a photosensitive agent is dispersed over a predetermined region of the inner surface. The container 10 is not particularly limited as long as it is a material that exhibits light transmittance. The container 10 may be composed of, for example, a photofunctional film or a photosensitive alloy film. The photofunctional film may refer to a film in which a photosensitive agent and a photocurable polymer resin are mixed. The photocurable polymer resin may include any one or more from the group consisting of silicone, latex, polyurethane, polyethylene terephthalate. By using a photocurable polymer resin, the container 10 can be manufactured by mixing the resin and the photosensitive agent to produce a photofunctional film.

The term "constant area" of the interior surface of the container 10 may mean including the bottom and / or side of the container 10, and in some cases both the bottom and / or side, or Some areas and / or some areas on the sides. For example, it includes a structure in which the region in which the photosensitive agent is dispersed and the region in which the photosensitive agent is not dispersed are repeated at the intersection.

Meanwhile, the photosensitive alloy film may refer to a film in which a photosensitive agent is ester-bonded to a metal surface. The metal may include any one or more selected from the group consisting of stainless steel, aluminum, magnesium, tin, titanium, tungsten and nickel.

The container 10 may serve to receive water to be sterilized or contaminated with water and harmful microorganisms. The size and volume of the container 10 are not particularly limited and can be appropriately set.

As an example, the size of the container 10 may be about 130 mm in width (A), about 100 mm in length (B), and about 20 mm in height (C), but is not limited thereto.

The container 10 including the photofunctional film or the photosensitive alloy film generates free radicals in oxygen by using light energy of a light source when irradiated with light, and the free radicals are harmful in water contained in the container 10. Microorganisms can be disinfected.

The harmful microorganism may include, for example, bacteria, food poisoning bacteria and the like. Specifically, the harmful microorganism may include any one or more selected from the group consisting of Escherichia coli, Staphylococcus aureus, cholera and Salmonella.

The partition wall is formed so that the flow of water to form a curved flow path, the first partition wall to form a flow path on one side inside the container (10); And a k + 1th partition that forms a flow path on an opposite side of the flow path formed by the first partition wall, wherein a position of the flow path formed by the kth partition wall is formed on the opposite side of the flow path formed by the k + 1th partition wall, k + 1 is n, k is an integer of 1 or more, and n may be 2 or more.

As one example, the flow path may be formed by the second partition wall opposite to the flow path formed by the first partition wall. At this time, the number n of partition walls is not particularly limited, and may be 2 to 30, 3 to 20, or 5 to 10. The water in the container 10 is introduced into the water inflow line 20 by the partition wall and may form an S-shaped curve while flowing out to the water outflow line 30.

The partition wall may have a structure in which a photosensitive agent is dispersed over a predetermined region of the inner surface. The partition wall is not particularly limited as long as the material exhibits light transmittance. The partition wall may be composed of, for example, a photofunctional film or a photosensitive alloy film. The photofunctional film may refer to a film in which a photosensitive agent and a photocurable polymer resin are mixed. The photocurable polymer resin may include any one or more from the group consisting of silicone, latex, polyurethane, polyethylene terephthalate. By using a photocurable polymer resin, the said resin and a photosensitive agent can be mixed, an optical functional film can be produced, and a partition can be manufactured.

In the present invention, the flow rate of water in the vessel 10 may be 0.01 to 0.1L / s or 0.05 to 0.08 L / s. If the flow rate of the water is in the above range, the water to be sterilized effectively increases the time to contact the photofunctional beads can easily perform the antibacterial and sterilization action by the photofunctional beads. At this time, the water can be circulated using a pump device having a flow rate of about 5L / min. The pump device facilitates circulation of the photofunctional beads 50 in the vessel 10 to facilitate further contamination prevention, recoverability and reuse. In addition, it is possible to control the light energy induced free radicals for killing harmful microorganisms.

In the present invention, the light source device 60 may irradiate light to at least one of the side, the bottom, and the top of the container 10, and serves to induce photodynamic inactivation (PDI) by irradiating light. do. As the light source device 60, a light emitting diode (LED), a laser xenon lamp, or the like can be used, for example, a green LED can be preferably used. The output (light quantity) of the light source device 60 may be, for example, 0.0001 to 20 mW / cm ² . Specifically, the output of the light source device 60 is 1 to 20 mW / cm ² , 1 to 15 mW / cm ² , 1 to 13 mW / cm ² , 1 to 10 mW / cm ² , 1 to 8 mW / cm ² , 0.1 to 8 mW / cm ² and 0.1 to 5 mW / cm ² .

In addition, the wavelength of the light source device 60 may be 400 to 700 nm. Specifically, the wavelength of the light source device 60 is 405 to 700 nm, 405 to 680 nm, 410 to 680 nm, 410 to 660 nm, 415 to 650 nm, 420 to 650 nm, 420 to 620 nm and 460 to 600 nm Can be. Since the photosensitive agent used in the present invention has a high absorption rate when the wavelength is 400 to 700 nm, the container 10, the partition 40, and the optical functional beads 50 using the light source device 60 in the above wavelength range are used. It can increase the efficacy of disinfecting harmful microorganisms.

The optical functional beads 50 may be to perform an antibacterial action by a light source supplied. In the present invention, the photofunctional beads may be those containing a photosensitizer in the polymer beads.

The optical functional beads 50 may be obtained by supporting the photosensitive agent in the polymer beads through a swelling method of absorbing the photosensitive solution in the polymer beads. The photofunctional beads 50 may be beads having bactericidal properties by active oxygen generated through photoenergy induction as polymer beads into which a photosensitive agent is introduced.

Specifically, the photosensitizer is tris (1,3-dichloroisopropyl) phosphate (Tris (1,3-dichloroisopropyl) phosphate, TDCPP), hematoporphyrin (HP), 5, 10, 15-triphenyl -20- (4-carboxyphenyl) -porphyrin platinum (5,10,15-triphenyl-20- (4-carboxyphenyl) -porphyrin platinum, PtCP), 5,15-bisphenyl-10,20-bis (4- Methoxycarbonylphenyl) -porphyrin platinum (5,15-bisphenyl-10,20-bis (4-methoxycarbonylphenyl) -porphyrin platinum, t-PtCP), tetraphenyl porphyrin (H ₂ TPP), protoporphyrin ( any one or more selected from the group consisting of proto porphyrin (PP), indocyanin green (ICG) and meso-tetrakis (p-sulfonatophenyl) porphyrin (TSPP) It may include, more specifically tetraphenyl porphyrin (tetraphenyl porphyrin, H ₂ TPP) may be included.

The polymer beads may be any one or more selected from the group consisting of polyurethane, polyurethane acrylate, and polyethylene phthalate. Preferably polypropylene can be used.

The diameter of the optical functional beads 50 may be 0.1 to 40mm. Specifically, the diameter of the optical functional beads 50 may be 0.1 to 35mm, 0.1 to 30mm, 0.5 to 30mm, 1.0 to 30mm, 0.5 to 25mm, 1.0 to 25mm, 1.5 to 25mm and 0.1 to 20mm. Optical functional beads 50 having a diameter in the above range are effective to disinfect water or sterilized objects to be sterilized without being entangled in water.

The optical functional beads may be elliptical in cross section, wherein the ratio of the short axis and the long axis of the cross section may range from 1: 1 to 1:10 or from 1: 1 to 1: 5. The photofunctional beads having this form can smoothly supply water.

The present invention provides a microbial disinfection method using the disinfection device.

Disinfection method according to the invention,

Introducing water to be sterilized into a water inflow line of the disinfection device according to the invention; And irradiating light.

As one example, the water to be disinfected may include a disinfectant, wherein the disinfectant may be at least one of vegetables and fruits. The disinfecting object is not particularly limited and may be used as long as it is food washed by water. Specifically, it may be agricultural products such as vegetables and fruits contaminated with harmful microorganisms.

In addition, the step of irradiating light may irradiate at least one of the side, bottom and top of the container 10 with a light source having a wavelength of 400 to 700nm. When the light source is irradiated, the photosensitive agent included in at least one of the vessel 10, the partition 40, and the photofunctional beads 50 may react with oxygen to generate active oxygen. The active oxygen may serve to sterilize harmful microorganisms in water or sterilization targets.

In the irradiating light, the light irradiation output may have a light irradiation time of 30 minutes or more. Specifically, it may be 30 to 200 minutes, 30 to 190 minutes, 35 to 180 minutes, 40 to 170 minutes, 45 to 160 minutes, 50 to 150 minutes, 55 to 140 minutes and 60 to 140 minutes. In the step of irradiating light, sufficient light may be irradiated to at least one kind of the container 10, the partition 40, and the photofunctional beads 50 for at least 30 minutes to sufficiently sterilize the water or sterilized object to be sterilized.

Hereinafter, the present invention will be described in more detail with reference to examples according to the present invention, but the scope of the present invention is not limited to the following examples.

Production Example  One

A photosensitizer solution was prepared by dissolving a photosensitizer tetraphenyl porphyrin (H ₂ TPP) in a solvent dichloromethane. Then, polypropylene beads were immersed in the photosensitizer solution at 60 ° C. for 24 hours to prepare photofunctional beads.

Production Example  2

The photocurable polymer resin polyurethane acrylate and the photosensitive tris (1,3-dichloroisopropyl) phosphate (TDCPP) were dissolved in a dichloromethane solvent to prepare a mixed solution. The mixed solution was poured into a rectangular mold and then irradiated with ultraviolet light to cure the polymer resin mixed solution to prepare a photofunctional film. After cutting the optical functional film to produce a container having a width of about 130 mm, a length of about 100 mm and a height of about 20 mm, five partition walls having a length of about 80 mm and a height of about 20 mm were formed in the container, and manufactured in Preparation Example 1 in the container. After storing the optical functional beads, a water inflow line and a water discharge line were formed on one side of the container to prepare a disinfection device as illustrated in FIGS. 1 and 2.

Example  One

Using the disinfection device prepared in Preparation Example 2, using the device and method of the specifications and conditions as shown in Table 1 below to evaluate the disinfection efficacy of the optical functional beads.

Sample H ₂ TPP Polymer Beads (B310) bacteria E. coli (ATCC 25922) Water Saline 1L Bacteria Resuspension Turbidity
(Bacteria resuspension turbidity) 0.5 (1.5x10 ⁸ CFU / ml) x1 / 100000 Flow rate 5L / min Experiment environment 25 ℃, Air Light source Green LED Quantity of light 2.9 mW / cm ² Survey time 0, 20, 40, 60 minutes Analytical Method Colony Count Method
(Colony count method, 100µl)

Example  2

Using the disinfection device prepared in Preparation Example 2, using the device and method of the specifications and conditions as shown in Table 2 to evaluate the disinfection efficacy of the optical functional beads.

Sample PPIX Zn (II) Polymer Beads (JM-380) bacteria E. coli (ATCC 25922) Water Saline 1L Bacteria Resuspension Turbidity
(Bacteria resuspension turbidity) 0.5 (1.5x10 ⁸ CFU / ml) x1 / 100000 Flow rate 5L / min Experiment environment 25 ℃, Air Light source Xe lamp (510nm cut-off filter) Quantity of light 12.4mW / cm ² Survey time 0, 20, 40, 60 minutes Analytical Method Colony Count Method
(Colony count method, 100µl)

Comparative example  1-1

The experiment was conducted in the same manner as in Table 1, except that the experiment was performed in a dark condition without a light source.

Comparative example  1-2

The experiment was conducted in the same manner as in Table 1 except that polypropylene beads containing no photosensitive agent were used instead of the photofunctional beads.

Comparative example  2-1

The experiment was conducted in the same manner as in Table 2, except that the experiment was performed in a dark condition without a light source.

Comparative example  2-2

The experiment was conducted in the same manner as in Table 2 except that polypropylene beads containing no photosensitizer in place of photofunctional beads and visible light instead of green LEDs were used.

Experimental Example  One

After the experiment according to Example 1, Comparative Example 1-1 and Comparative Example 1-2 proceeded to each experiment proceeding 0, 20, 40, 60 minutes after plating 100μL of wash water on a solid medium (plating) It was incubated in dark, 35 ° C conditions for hours. Thereafter, the number of bacteria was confirmed. The results are shown in FIG. 3. Experimental results When the visible light is irradiated in a dark place or a specific wavelength as in Comparative Example 1-1 (FIG. 3 b) and Comparative Example 1-2 (c of FIG. 3), E. Although no decrease in the number of coli was apparent, the conditions according to Example 1 (FIG. 3 a) decreased to 0 cfu in 60 minutes. This absorbing tetraphenyl porphyrin (H ₂ TPP) the wavelength of the light source of the green LED area photo-functional bead comprising the free radicals generated by means that the sterilizing microorganisms.

Experimental Example  2

After the experiment according to Example 2, Comparative Example 2-1 and Comparative Example 2-2 proceeded to each experiment proceeding 0, 20, 40, 60 minutes after plating 100μL of wash water on a solid medium (plating) It was incubated in dark, 35 ° C conditions for hours. Thereafter, the number of bacteria was confirmed. The results are shown in FIG. 4. Experimental results When the visible light was irradiated in a dark place or a specific wavelength as in Comparative Example 2-1 (FIG. 3 e) and Comparative Example 2-2 (FIG. 3 f), E. Although no decrease in the number of coli was apparent, the conditions according to Example 2 (d in FIG. 3) decreased to 0 cfu in 60 minutes. This absorbing tetraphenyl porphyrin (H ₂ TPP) the wavelength of the light source of the green LED area photo-functional bead comprising the free radicals generated by means that the sterilizing microorganisms.

Therefore, it was confirmed that the disinfection device and the disinfection method according to the present invention can effectively sterilize harmful microorganisms.

10: Container
20: water inlet line
30: water outflow line
40: bulkhead
50: photofunctional beads
60: light source device

Claims (8)

A container containing water;
A water inlet line formed at one side of the container and supplying water to be sterilized;
Water discharge line is formed on another side of the container, for discharging the sterilized water;
A partition wall formed inside the container to control the flow path of the water;
A photofunctional bead located between the partition walls in the container; And
A disinfection device including a light source device that supplies light at a distance from the container,
The optical functional beads contain a photosensitizer in the polymer beads, have a diameter of 0.1 to 35 mm, the ratio of the short axis and the long axis in the elliptical cross section has a ratio of 1: 1 to 1:10.
The method of claim 1,
The partition wall is formed so that the flow of water forms a curved flow path,
A first partition wall forming a flow path on one side of the container; And
A k + 1th partition wall forming a flow path on an opposite side of the flow path formed by the first partition wall,
The position of the flow path formed by the kth partition is formed on the opposite side of the flow path formed by the k + 1th partition,
The k + 1 is n, k is an integer of 1 or more, n is an integer of 2 or more.
The method of claim 1,
Disinfection device, characterized in that the flow rate of water in the container is 0.01 to 0.1L / s.
The method of claim 1,
Disinfection device, characterized in that the wavelength of the light source is 400 to 700 nm.
The method of claim 1,
Photofunctional beads are sterilizing device characterized in that to perform the antibacterial action by the light source supplied.
delete The method of claim 1,
Photosensitizers include tris (1,3-dichloroisopropyl) phosphate (TDCPP), hematoporphyrin (HP), 5,10,15-triphenyl-20- (4-carboxyphenyl) -porphyrin platinum (5,10,15-triphenyl-20- (4-carboxyphenyl) -porphyrin platinum, PtCP), 5,15-bisphenyl-10,20-bis (4-methoxycarba Bonylphenyl) -porphyrin platinum (5,15-bisphenyl-10,20-bis (4-methoxycarbonylphenyl) -porphyrin platinum, t-PtCP), tetraphenyl porphyrin (H ₂ TPP), proto porphyrin (proto porphyrin, PP), indocyanin green (ICG) and meso-tetrakis (p-sulfonatophenyl) porphyrin (meso-tetrakis (psulfonatophenyl) porphyrin (TSPP) containing any one or more selected from the group consisting of Disinfection device.
The method of claim 1,
Polymeric beads are any one or more selected from the group consisting of polypropylene, polyurethane, polyurethane acrylate, polyethylene phthalate.

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