KR102037891B1 - Multiple discrimination device and method for tumor discrimination - Google Patents

Multiple discrimination device and method for tumor discrimination Download PDF

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KR102037891B1
KR102037891B1 KR1020120116207A KR20120116207A KR102037891B1 KR 102037891 B1 KR102037891 B1 KR 102037891B1 KR 1020120116207 A KR1020120116207 A KR 1020120116207A KR 20120116207 A KR20120116207 A KR 20120116207A KR 102037891 B1 KR102037891 B1 KR 102037891B1
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channel
cancer cells
mixed solution
ferromagnetic pattern
ferromagnetic
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KR20130103282A (en
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조성목
이대식
박정원
정문연
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한국전자통신연구원
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces

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Abstract

The present invention provides a multiple separation device and a method for separating cancer cells in the blood using the same. In this apparatus and method, a blood sample is placed in a microchannel and the magnetic force of its flow rate or ferromagnetic pattern is adjusted to separate cancer cells according to the type of cancer.

Figure R1020120116207

Description

Multiple discrimination device and method for tumor discrimination

The present invention relates to a multiple separation device and a method for separating cancer cells in the blood, including species.

Separation of cell types or intracellular components is required as a preparatory tool for end purposes or other analyses in the fields of diagnosis, treatment and research in the medical field. For example, it is necessary to analyze cancer cells. Blood cancer cells in the blood collectively refer to cancer cells present in the peripheral blood of cancer patients and are cancer cells dropped from primary or metastatic lesions. These blood cancer cells are expected to be a potent biomarker in cancer diagnosis, treatment prognosis analysis, microtransition analysis and the like. In addition, blood cancer cell analysis is very promising as a future cancer diagnosis method because it has the advantage of non-invasive method compared to conventional cancer diagnosis method. However, blood cancer cells are very difficult to accurately analyze and require very sophisticated analytical methods because the distribution rate in blood is very low, such as 1 cancer cell per 1 billion cells or 1 cancer cell per 10 6-10 7 leukocytes.

Various methods have been studied as a method for separating cancer cells in blood, but the test takes a long time and only provides information on the presence and quantity of cancer cells, and it is difficult to analyze cancer types. In addition, interference by nonspecifically bound blood cells becomes a problem.

Therefore, an object of the present invention is to provide a multiple separation device and a method for separating cancer cells in blood, including species.

Multiple separation apparatus according to the present invention for achieving the above object, the channel through which the mixed solution flows; And a ferromagnetic pattern disposed below the bottom of the channel, wherein the flow rate of the mixed solution or the magnetic force of the ferromagnetic pattern may vary depending on the position of the channel.

In one example, the multiple separation device may further include an inlet through which the mixed solution is input and an outlet through which the mixed solution is discharged. The flow rate of the mixed solution is faster and closer to the inlet side. Closer to the outlet can be slower.

The width of the channel may be wider as it approaches the outlet from the inlet. At this time, the magnetic force of the ferromagnetic pattern may be constant according to the position.

The multiple separation device may further include at least one permanent magnet disposed adjacent to the channel.

The permanent magnet may be disposed below or next to the channel.

The mixed solution includes a first material species having a first magnetization amount, a second material species having a second magnetization amount, and a third material species having a third magnetization amount, wherein the second magnetization amount is the first material species. Greater than the magnetization and less than the third magnetization, the third species are trapped in the ferromagnetic pattern adjacent the inlet, the first species are trapped in the ferromagnetic pattern adjacent to the outlet or discharged to the outlet, The second material species may be trapped in the ferromagnetic pattern between the inlet and the outlet.

In a specific example, the mixed solution may be blood, the first species may be normal cells, and the second species and the third species may be different kinds of cancer cells to which magnetic nanoparticles are bound.

The cancer cells may include different numbers of markers, the magnetic nanoparticles may bind to the markers, and the second and third species may include different numbers of magnetic nanoparticles. have.

In the multiple separation device according to another embodiment of the present invention, the magnetic force of the ferromagnetic pattern is changed according to the position of the channel, the flow rate of the mixed solution may be constant according to the position of the channel. Specifically, the distance between the ferromagnetic pattern and the bottom of the channel may be changed depending on the position to change the magnetic force of the ferromagnetic pattern on the mixed fluid. The multiple separation device further includes an inlet through which the mixed solution is input and an outlet through which the mixed solution is discharged, wherein the distance between the ferromagnetic pattern and the bottom of the channel is closer to the inlet and is closer to the outlet. The closer you can be.

In the blood cancer cell separation method according to the present invention for achieving the above another object, a mixed solution comprising cancer cells to which the magnetic nanoparticles are bound by mixing the magnetic nanoparticles to which the antibody specifically reacts with the cancer cells and blood to be tested Preparing a; Placing the mixed solution into a channel in which ferromagnetic patterns are disposed on the bottom surface; And capturing cancer cells according to the type by adjusting the flow rate of the mixed solution in the channel or changing the magnetic force of the ferromagnetic pattern.

The method may further comprise analyzing / identifying cancer cells according to the capture position of the ferromagnetic pattern.

Analyzing / identifying the cancer cells according to the capture position of the ferromagnetic pattern may include removing the mixed solution remaining in the channel; Separating the cancer cells; And analyzing DNA of the cancer cells.

Separating the cancer cells from the surface of the ferromagnetic pattern may include demagnetizing the ferromagnetic pattern by applying a magnetic field in a direction opposite to a magnetic field in which the ferromagnetic pattern is magnetized. In this case, the magnetic field may have a size corresponding to a coercive field of the ferromagnetic pattern.

Demagnetizing the ferromagnetic pattern may use a permanent magnet or an electromagnet.

In the multiple separation device and blood cancer cell separation method according to an embodiment of the present invention, it is possible to easily diagnose the cancer occurrence and to classify the cancer. In addition, since the interference effects of blood cells can be almost completely eliminated, specificity can be greatly improved compared to existing technologies.

1 is a flow chart showing a method for separating cancer cells in the blood according to an embodiment of the present invention.
2 shows material species included in the mixed solution according to an embodiment of the present invention.
3A is a plan view showing a multiple separation device according to Embodiment 1 of the present invention.
3B and 3C are cross-sectional views of FIG. 3A taken along line A-A 'and B-B', respectively.
FIG. 4 shows the movement of species particles in the multiple separation device of FIG. 3A.
5A is a plan view showing a multiple separation device according to Embodiment 2 of the present invention.
5B is a cross-sectional view taken along the line AA ′ of FIG. 5A, respectively.
6A is a plan view showing a multiple separation device according to Embodiment 3 of the present invention.
6B is a cross-sectional view taken along the line AA ′ of FIG. 6A, respectively.
7 and 8 are cross-sectional views of multiple separation apparatus according to other examples of the invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. As those skilled in the art would realize, the described embodiments may be modified in various ways, and various modifications may be made. Only, the description of the embodiments are provided to make the disclosure of the present invention complete and to fully inform the person skilled in the art the scope of the present invention. In the accompanying drawings, for convenience of description, the size of the components is larger than the actual drawings, and the ratio of each component may be exaggerated or reduced.

If a component is said to be "on" or "connected" to another component, it may be directly in contact with or connected to another component, but it is understood that another component may exist in between. Should be. On the other hand, if a component is described as "directly on" or "directly connected" to another component, it may be understood that there is no other component in between. Other expressions describing the relationship between the components, such as "between" and "directly between", and the like, may likewise be interpreted.

Terms such as first and second may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as the second component, and similarly, the second component may also be referred to as the first component.

Singular expressions include plural expressions unless the context clearly indicates otherwise. The terms "comprise" or "having" are intended to indicate that there is a feature, number, step, operation, component, part, or combination thereof described on the specification, and that one or more other features or numbers, It may be interpreted that steps, actions, components, parts or combinations thereof may be added.

Unless otherwise defined, terms used in the embodiments of the present invention may be interpreted as meanings commonly known to those of ordinary skill in the art. In addition, “at least one” is used in the same sense as at least one and may optionally refer to one or more.

1 is a flow chart showing a method for separating cancer cells in the blood according to an embodiment of the present invention. 2 shows material species included in the mixed solution according to an embodiment of the present invention. 3A is a plan view showing a multiple separation device according to Embodiment 1 of the present invention. 3B and 3C are cross-sectional views of FIG. 3A taken along line A-A 'and B-B', respectively.

1 and 2, in the blood cancer cell separation method according to an embodiment of the present invention, magnetic nanoparticles bound to antibodies specific for cancer cells are mixed with blood to be tested to prepare a mixed solution (first step). , S10). The blood may include normal cells such as leukocytes (first species, PU), different types of cancer cells A (second species, PS1) and cancer cells B (third species, PS2). When the types of cancer cells PS2 and PS3 are different, the number of markers (eg, antigens) expressed in the cancer cells is different. EpCAM markers represent about 500,000 EpCAM expressions per cell in breast cancer cell SKBr-3, about 50,000 EpCAM expressions per cell in prostate cancer cell PC3-9, and about 30 cells in bladder cancer cell T-24. The number of EpCAM expressions is about 2,000, and the number of markers expressed per cancer cell varies greatly depending on the carcinoma. Therefore, when the antibody that specifically reacts to EpCAM is bound to the magnetic nanoparticles and the magnetic nanoparticles and the blood of the cancer patient are mixed, a large difference occurs in the number of magnetic nanoparticles that are bound to the cancer cells according to the cancer types of the cancer cells. The difference in the number of magnetic nanoparticles bound per cell can be utilized to separate carcinomas using a magnetic field. The greater the number of magnetic nanoparticles, the greater the magnetization. The magnetic nanoparticles may nonspecifically bind to normal cells such as white blood cells as in FIG. 2, but the number of magnetic nanoparticles bound to white blood cells may be significantly smaller than the number of magnetic nanoparticles bound to markers of cancer cells. If the first material species PS1, the second material species PS2, and the third material species PS3 have a first magnetization amount, a second magnetization amount, and a third magnetization amount, respectively, the second magnetization amount Is greater than the first magnetization and less than the third magnetization.

In this way, the mixed solution consisting of blood containing the magnetic nanoparticles is separated using the multiple separation apparatus of Examples 1 to 3.

<Example 1>

3A to 3C, the multiple separation apparatus 100 according to the first embodiment of the present invention includes an inlet (1) into which the mixed solution is input, an outlet 4 through which the mixed solution is discharged, and the inlet ( 1) and a channel 2 connecting the outlet 4. In the channel 2, the mixed solution flows in the first direction X. The channel 2 is connected to the outlet 4; It may be provided by the substrate 6 and the lid 5 covering the substrate 6 and providing the inlet 1 and the groove 5. The substrate 6 and the lid 5 are less reactive. It may be formed of a material such as glass or plastic.

Ferromagnetic patterns 3 are disposed below the bottom surface of the channel 2. The ferromagnetic pattern 3 may be any material having ferromagnetic properties. Typically, Ni, Co, Fe, or compounds thereof may be used. The ferromagnetic pattern 3 serves to attract the magnetic particles in the fluid to hinder the flow of the particles.

It is preferable to dispose the permanent magnet 7 under the substrate 6 in order to magnetize the ferromagnetic pattern 3 and keep the magnetization state constant. The permanent magnet 7 may include a first pole P1 and a second pole P2 opposite to each other. The first pole P1 may be, for example, an N pole, and the second pole P2 may be, for example, an S pole. The ferromagnetic patterns 3 have an elongated bar shape in a second direction Y crossing the first direction X and are spaced at regular intervals with the same size. The ferromagnetic patterns 3 may have the same magnetic force.

The mixed solution includes a first species (PS1) that is normal cells, a second species (PS2) that is cancer cell A, and a third species (PS3) that is cancer cell B. In the present embodiment, but two types of cancer cells are illustrated, three or more are possible.

1 and 3A to 3C, the mixed solution is introduced into the inlet 1. That is, the ferromagnetic patterns 3 having a constant magnetic force in accordance with the position is put into the channel 2 disposed on the lower surface (S20). The cancer cells are captured according to the type by controlling the flow rate of the mixed solution or the magnetic force of the ferromagnetic pattern 3 in the channel (S30). Examples 1 and 2 disclose a method of capturing cancer cells according to the type by adjusting the flow rate of the mixed solution, and Example 3 discloses a method of capturing cancer cells according to the type by controlling the magnetic force of the ferromagnetic pattern applied to the mixed solution. .

Referring again to FIGS. 3A-3C, the widths W1, Wn of the channel 2 widen toward the outlet 4 from the inlet 1. That is, the width W1 of the channel 2 adjacent to the inlet 1 is narrower than the width Wn of the channel 2 adjacent to the outlet 4. The widths W1 and Wn of the channel 2 may be continuously widened from the inlet 1 toward the outlet 4. In all positions the bottom surface of the channel 2 may be flat and the thickness of the channel 2 may be constant. The flow rates v 1, v n of the mixed solution in the channel 2 are slowed from the inlet 1 to the outlet 4. That is, the flow rate v 1 of the mixed solution adjacent to the inlet 1 is faster than the flow rate v n of the mixed solution adjacent to the outlet 4.

FIG. 4 shows the movement of species particles in the multiple separation device of FIG. 3A.

1, 3A to 3C and 4, the force applied to the material species PS1, PS2, and PS3 adjacent to the ferromagnetic pattern 3 may be determined by the ferromagnetic pattern 3 being the material species PS1,. The magnetic force F m that attracts (to capture) PS2, PS3 and the force F d by the flow of the mixed solution in the opposite direction act. As the number of magnetic nanoparticles bonded to the material species PS1, PS2, and PS3 increases, the magnetization amount increases, and thus, the magnetization amount is attracted to the ferromagnetic pattern 3 well. Thus, for example, the magnetic force (F m ) acting on the third material species (PS3) having the largest magnetization amount may be greater than the force (F d ) due to the flow of the mixed solution, so that it is adjacent to the inlet (1) The probability of being trapped in the ferromagnetic patterns 3 becomes large. However, since the flow velocity is relatively high at this position, the flow of the mixed solution rather than the magnetic force F m is applied to the first and second species PS1 and PS2 having a smaller magnetization amount than the third species PS3. Force F d influences more, causing it to float along the channel 2. Then, the second material species PS2 may be captured in the ferromagnetic pattern 3 having a weaker flow rate. The first material species PS1 having the weakest magnetization amount may be trapped in the ferromagnetic pattern 3 adjacent to the outlet 4 or discharged to the outlet 4 without being captured. This can separate cancer cells into carcinomas according to the number of magnetic nanoparticles.

Subsequently, referring to FIG. 1, cancer cells are analyzed / identified according to the capture position of the ferromagnetic pattern 3 (S40). Specifically, after all the blood samples to be tested are flowed out, residual blood remaining in the channel 2 is removed by using a buffer solution such as saline solution. The flow rate of the buffer solution should be the same or weaker than the blood to prevent the escape or damage of the captured cancer cells.

After removing the remaining blood, it is possible to estimate the presence and type of cancer cells by analyzing the number and location of the cancer cells captured through image analysis of the chip captured by the cancer cells. At this time, since the capture position of the cancer cells is localized around the ferromagnetic pattern (3), it is sufficient to analyze only a narrow area around the ferromagnetic pattern during image analysis.

If additional DNA analysis is required using the captured cancer cells, the captured cancer cells should be collected separately from the ferromagnetic pattern (3). In order to separate and capture cancer cells captured by the ferromagnetic pattern 3, the ferromagnetic pattern 3 must be demagnetized. To this end, it is possible to demagnetize the ferromagnetic pattern 3 by applying a weak magnetic field in the opposite direction in which the ferromagnetic pattern 3 is magnetized. The demagnetization of the ferromagnetic pattern 3 can be performed by removing the lower permanent magnet 7 used in capturing cancer cells and arranging a magnet of a weak size in the opposite direction. At this time, the magnetic field applied to the ferromagnetic pattern 3 by the weak magnet should be a size corresponding to the characteristic coercive field of the magnetic material constituting the ferromagnetic pattern (3). Electromagnets can be used when fine adjustment of the applied magnetic field using a permanent magnet is difficult at the time of demagnetization of the ferromagnetic pattern 3. Remove the lower magnet and place the electromagnet to have the magnetic field in the opposite direction at the same position, and check the separation of the captured cells by gradually increasing the applied magnetic field by controlling the amount of current flowing through the electromagnet while flowing a buffer solution such as saline solution. Can be. The magnitude of the magnetic field at the moment when the captured cells are separated from the ferromagnetic pattern 3 is close to the coercive force of the pattern 3, and it is preferable to detach the cells at that size. When an excessively large magnetic field is applied, the ferromagnetic pattern 3 is magnetized in the opposite direction and attracts the detached cancer cells again. Therefore, the magnetic field applied when the cancer cells are detached should be maintained near the coercive force of the ferromagnetic pattern 3. The coercive force of the ferromagnetic material varies depending on the ferromagnetic material used, so it must be adapted to the ferromagnetic material used.

<Example 2>

5A is a plan view showing a multiple separation device according to Embodiment 2 of the present invention. 5B is a cross-sectional view taken along the line AA ′ of FIG. 5A, respectively.

5A and 5B, in the multiple separation apparatus 101 according to the second exemplary embodiment, the planar shape of the channel 2 has a curved concave-convex structure, and the widths W1 and Wn of the channel 2 are stepwise. As it is discontinuously widened. The ferromagnetic pattern 3 may have a plurality of line shapes that cross the channel 2 and extend in the first direction X. FIG. Depending on the location, the bottom surface of the channel 2 may be flat and the thickness may be constant. Since the widths W1 and Wn of the channel 2 increase, the flow velocities v 1 and v n of the mixed solution through them also become slower from the inlet 1 to the outlet 4. In this embodiment, there is an advantage that the reliability of the operation can be improved by arranging several ferromagnetic patterns 3 in the width section of the same flow path, that is, the same flow rate section. As described above, cancer cells can be separated into carcinomas according to the number of magnetic nanoparticles.

<Example 3>

6A is a plan view showing a multiple separation device according to Embodiment 3 of the present invention. 6B is a cross-sectional view taken along the line AA ′ of FIG. 6A, respectively.

6A and 6B, in the multiple separation apparatus 102 according to the third exemplary embodiment, the widths W1 and Wn and the thickness of the channel 2 may be constant according to positions. The channel 2 may be inclined. However, the top surfaces of the ferromagnetic patterns 3 are coplanar with the top surfaces of the substrate 6 and are horizontal. Therefore, the distance between the ferromagnetic patterns 3 and the lower surface of the channel 2 may become narrower from the inlet 1 to the outlet 4. Even when the channel 2 is inclined, the amount of the mixed fluid flowing in the channel 2 is so small that it is hardly influenced by gravity. Therefore, since the width and thickness of the channel 2 are constant, the flow velocity of the mixed fluid flowing in the channel 2 may be substantially constant depending on the position. However, the trapping force (or magnetic force (F m ) in FIG. 4) of the ferromagnetic pattern 3 on the mixed fluid of the channel 2 may increase from the inlet 1 to the outlet 4. . Therefore, as described with reference to FIG. 4, the first and second material species PS1 and PS2 having a smaller magnetization amount than the third material species PS3 are caused by the flow of the mixed solution rather than the magnetic force F m . The force F d affects more, causing it to float along the channel 2. In the position where the magnetic force is stronger, the second material species PS2 may be captured in the ferromagnetic pattern 3. The first material species PS1 having the weakest magnetization amount may be trapped in the ferromagnetic pattern 3 adjacent to the outlet 4 or discharged to the outlet 4 without being captured. This can separate cancer cells into carcinomas according to the number of magnetic nanoparticles.

7 and 8 are cross-sectional views of multiple separation apparatus according to other examples of the invention.

Referring to FIG. 7, in the multiple separation apparatus 103 according to the present example, two permanent magnets 7 may be disposed at both sides of the channel 2, respectively. Alternatively, referring to FIG. 8, in the multiple separation device 104 according to the present example, two permanent magnets 7 may be disposed below both sides of the channel 2, respectively. 3b, 5b, 6b and 8, when the permanent magnet 7 is located below the channel 2, the strength of the magnetic field weakens the farther away from the bottom surface of the channel (2) Species PS2 and PS3 are moved near the bottom surface of the channel 2, so that the species PS2 and PS3 can be well captured without variation in the channel height in the channel 2. The permanent magnet 7 may be arranged in various ways. Combinations of embodiments of the invention are also possible.

The embodiments of the present invention described above are not implemented only through the apparatus and the method, and such an implementation can be easily implemented by those skilled in the art from the description of the above-described embodiments.

Claims (18)

A channel through which the mixed solution flows;
An inlet connected to the channel and into which the mixed solution is introduced;
An outlet through which the mixed solution is discharged; And
A ferromagnetic pattern disposed below the bottom of the channel,
The flow rate of the mixed solution varies with the position of the channel,
The flow rate of the mixed solution is faster the closer to the inlet side, the slower the closer to the outlet,
The mixed solution includes a first material species having a first magnetization amount, a second material species having a second magnetization amount, and a third material species having a third magnetization amount,
The second magnetization amount is larger than the first magnetization amount and smaller than the third magnetization amount,
The third material species is trapped in a ferromagnetic pattern adjacent the inlet,
The first material species is trapped in the ferromagnetic pattern adjacent to the outlet or discharged to the outlet,
The second material species is trapped in the ferromagnetic pattern between the inlet and the outlet,
The mixed solution is blood,
The first species is normal cells,
The second species and the third species are different kinds of cancer cells combined with magnetic nanoparticles,
The cancer cells comprise different numbers of markers, the magnetic nanoparticles are bound to the markers,
And the second material species and the third material species comprise different numbers of magnetic nanoparticles. delete The method of claim 1,
And the width of the channel is wider as it is closer to the outlet from the inlet. The method of claim 1,
The magnetic separation force of the ferromagnetic pattern is a constant multiple separation device. The method of claim 1,
And at least one permanent magnet disposed adjacent said channel. The method of claim 5,
And the permanent magnet is disposed below or beside the channel. delete delete delete The method of claim 1,
And the magnetic force of the ferromagnetic pattern varies with the position of the channel. The method of claim 1,
And the distance between the ferromagnetic pattern and the bottom of the channel varies with location. The method of claim 11,
The multiple separation device further includes an inlet through which the mixed solution is input and an outlet through which the mixed solution is discharged, connected to the channel.
And the distance between the ferromagnetic pattern and the bottom of the channel narrows toward the outlet from the inlet. Preparing a mixed solution comprising cancer cells to which the magnetic nanoparticles are bound by mixing magnetic nanoparticles to which the antibody specifically reacts to cancer cells and blood to be tested;
Placing the mixed solution into a channel in which ferromagnetic patterns are disposed on the bottom surface; And
Plasma cancer cell separation comprising the step of capturing cancer cells according to the type by adjusting the flow rate of the mixed solution in the channel. The method of claim 13,
The method for separating cancer cells in the blood further comprising the step of analyzing / discriminating the cancer cells according to the capture position of the ferromagnetic pattern. The method of claim 14,
Analyzing / identifying the cancer cells according to the capture position of the ferromagnetic pattern,
Removing the mixed solution remaining in the channel;
Separating the cancer cells; And
Blood cancer cell separation method comprising the step of analyzing the DNA of the cancer cells. The method of claim 15,
Separating the cancer cells from the surface of the ferromagnetic pattern,
And demagnetizing the ferromagnetic pattern by applying a magnetic field in a direction opposite to a magnetic field in which the ferromagnetic pattern is magnetized. The method of claim 16,
The magnetic field is a method for separating cancer cells in the blood having a size corresponding to the coercive field (coercive field) of the ferromagnetic pattern. The method of claim 16,
Demagnetizing the ferromagnetic pattern is blood cancer cell separation method using a permanent magnet or electromagnet.
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KR101583017B1 (en) * 2015-07-17 2016-01-06 주식회사 지노바이오 MIP(Magnetic Iron Particles) Discrimination System
KR101720609B1 (en) 2015-07-17 2017-03-28 주식회사 지노바이오 MIP(Magnetic Iron Particles) Discrimination Device Using Magnetic Force Flow
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