KR102029531B1 - Preparation method of pickled foods - Google Patents

Preparation method of pickled foods Download PDF

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KR102029531B1
KR102029531B1 KR1020170050320A KR20170050320A KR102029531B1 KR 102029531 B1 KR102029531 B1 KR 102029531B1 KR 1020170050320 A KR1020170050320 A KR 1020170050320A KR 20170050320 A KR20170050320 A KR 20170050320A KR 102029531 B1 KR102029531 B1 KR 102029531B1
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pickles
pickled
extract
raw material
preparation
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KR1020170050320A
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KR20180117341A (en
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전향미
김명숙
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충주시
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/20Products from fruits or vegetables; Preparation or treatment thereof by pickling, e.g. sauerkraut or pickles
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • A23V2200/328Foods, ingredients or supplements having a functional effect on health having effect on glycaemic control and diabetes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts

Abstract

본 발명은 여주, 뽕잎 및 새싹보리의 군에서 선택된 적어도 1종의 식물추출액을 얻는 단계; 상기 얻어진 식물추출액을 절임의 대상이 되는 원료에 담금수로 첨가하여 담그는 단계; 및 상기 담금수에 담근 절임의 대상이 되는 원료를 숙성하여 절임식품을 제조하는 단계;를 포함하는 절임식품의 제조방법을 제공한다.The present invention comprises the steps of obtaining at least one plant extract selected from the group of bitter gourd, mulberry leaf and sprout barley; Immersing the obtained plant extract by adding immersion water to a raw material to be pickled; It provides a method for producing a pickled food comprising; and aging the raw material to be pickled in the immersion water to produce a pickled food.

Description

식물추출액 함유 담금수를 이용한 절임식품의 제조방법{PREPARATION METHOD OF PICKLED FOODS}Manufacturing method of pickled food using plant extract immersion water {PREPARATION METHOD OF PICKLED FOODS}

본 발명은 절임식품의 제조방법에 관한 것으로, 보다 상세하게는 항당뇨에 도움을 주는 뽕잎, 여주 및 새싹보리 추출액을 이용하여 용수를 제조하고, 염도가 낮은(2% 미만) 절임식품 예로, 저염장아찌 및 저염피클을 제조하여 관능적 품질과 저장성이 우수한 식물추출액 함유 담금수를 이용한 절임식품의 제조방법에 관한 것이다.
The present invention relates to a method for preparing pickled food, and more specifically, to prepare water using mulberry leaves, bitter melon and sprout barley extract to help anti-diabetic, low salt (less than 2%) pickled food example, low salt The present invention relates to a method for preparing pickled food using immersion water containing botanical extracts having excellent organoleptic quality and shelf life by preparing pickles and low salt pickles.

여주는 우리나라의 전역에서 자연적으로 자라며, 또는 재배되기도 한다. 줄기는 가늘고 길이 3∼7m로 자라며 더 크게 자랄 수도 있다. 잎은 어긋나고 잎자루가 길며, 열매는 긴 타원형이고 양끝이 좁으며 혹같은 돌기가 있고 처음엔 녹색이나 익어감에 따라 연두색, 연록색, 황적색으로 익으면 불규칙하게 갈라져서 선홍색 육질로 싸인 종자가 나온다. 어린 열매와 홍색 종피(種皮)는 주로 식용으로 하고 종자는 주로 약용으로 사용되어 왔으며, 당뇨병 환자에서 혈당강하효과가 있는 것으로 알려져 왔다(Grover and Yadav, 2004).Yeoju grows or grows naturally throughout Korea. Stems are thin, grow to 3-7m long, and may grow larger. The leaves are alternate, the petioles are long, the fruit is long oval, the both ends are narrow, and the bumps are bumps. At first, it grows green, ripe green or yellow red depending on the ripening. Young fruits and red paprika are mainly edible and seeds have been used mainly for medicinal purposes and have been known to have a hypoglycemic effect in diabetics (Grover and Yadav , 2004).

보리(Hordeum vulgare L.)에는 lutonarin, saponarin 등 다양한 폴리페놀과 플라보노이드가 함유되어 있으며, 수용성 식이섬유인 β-glycan, 아미노산, 탄수화물이 함유되어 있다. 또한, 보리 잎 중 풍부하게 들어있는 superoxide dismutase (SOD)와 보리잎에서 분리된 glycoprotein인 P4-D1, D1-G1은 강한 항염증작용에 대해 알려져 있으며(Lee et al, 2014), 항산화 및 항염증 효과를 가진 천연물 소재로 활용 가능하다(Eun et al, 2016).Barley (Hordeum vulgare L.) There is contained a variety of polyphenols and flavonoids such as lutonarin, saponarin, and may contain water-soluble dietary fiber is β-glycan, amino acids, carbohydrates. In addition, superoxide dismutase (SOD) abundant in barley leaves and glycoproteins P4-D1 and D1-G1 isolated from barley leaves are known for their strong anti-inflammatory activity (Lee et al, 2014). It can be used as a natural material with effects (Eun et al , 2016).

뽕잎(Morus alba leaf)은 항당뇨 소재로 일반인들에게 많이 알려져 있다. 뽕나무의 뽕잎은 다류 제품으로 많이 이용되고 있는데 뽕잎이 항산화 및 지방축적 억제효과가 있다는 연구가 보고되고 있어 기능성 식품소재로 그 가치가 높다(Choi et al, 1999. Do et al. 2011). Morus alba leaf is known to the general public as an antidiabetic material. Mulberry leaves of mulberry tree are widely used as a multi-product product, and research has been reported that mulberry leaves have antioxidant and fat accumulation inhibitory effects, which is highly valuable as a functional food material (Choi et al , 1999. Do et al. 2011).

더군다나 뽕잎은 병충해에 강해서 농약 및 충 제거제를 살포하지 않아도 잘 자랄 뿐만 아니라 전국적으로 널리 분포되어 있어 쉽게 구할 수 있다는 이점이 있기 때문에 이러한 뽕잎의 효능을 이용한 장아찌 제조방법이 공개 및 등록 특허에 개시되어 있다.Moreover, mulberry leaves are resistant to pests, so they can grow well without spraying pesticides and detoxifiers, and are widely distributed throughout the country, making them easy to obtain. Therefore, methods for preparing pickles using the efficacy of such mulberry leaves are disclosed in published and registered patents. .

이러한 종래의 뽕잎, 여주 및 새싹보리를 이용한 장아찌들은 대부분 간장, 고추장, 된장을 이용한 양념장에 버무려 숙성시켜 취식하도록 되어 있거나, 뽕잎, 여주 및 새싹보리에 혼합될 양념장을 제조할 때 숙성과정에서 연잎, 오미자 등을 추가하여 맛은 부드럽게 하고 영양을 극대화할 수 있는 효능들을 갖는 내용도 일부 개시되어 있다.Most of the conventional pickles using mulberry leaves, bitter melon and sprout barley are mixed with seasonings using soy sauce, red pepper paste and soybean paste to be ripened or eaten, or when preparing seasonings to be mixed with mulberry leaves, bitter melon and sprout barley in the ripening process. The addition of Schizandra chinensis and the like have some contents that have the effects of softening the taste and maximizing nutrition.

일반적으로 장아찌는 각 지역이나 계절에 따라 생산되는 우리나라 고유의 식품으로 중요한 밑반찬 역할을 담당하여 왔으며, 일반적으로 판매되고 있는 장아찌의 평균 염도는 5~6%(저염 장아찌의 경우 염도는 약 3%내외)으로 김치, 젓갈 등과 함께 고염식품군에 속한다. 우리나라 사람들의 하루 평균 나트륨 섭취량은 5,280 mg으로 WHO 권고기준인 2,000 mg의 2.5배 이상으로 나타났다. 나트륨 과잉섭취는 혈압상승의 주요인으로서 소금이 주원인 식품으로 인정되어 왔다. 이와 같은 종래의 장아찌는 자주 반복적으로 먹을 경우 비만, 성인병, 당뇨병, 뇌졸중, 심근경색증, 신장병 등의 원인이 되는 문제점이 있다.In general, pickles are unique foods produced in each region and season, and they have played an important role as a side dish.In general, the average salinity of pickles sold is 5 ~ 6% (in the case of low salt pickles, the salinity is around 3%). ) Belongs to the high salt food group along with kimchi and salted fish. The average daily sodium intake of Korean people is 5,280 mg, which is more than 2.5 times the WHO recommended standard of 2,000 mg. Sodium overdose has been recognized as a major food source of salt as a major cause of blood pressure rise. Such conventional pickles have a problem that causes frequent obesity, adult disease, diabetes mellitus, stroke, myocardial infarction, kidney disease and the like.

도 1에 도시한 바와 같은 일반적인 절임식품의 제조공정에서, 고춧가루, 고추장, 간장, 설탕, 물엿 및 물을 혼합하여 양념장을 만들거나, 다시마, 멸치, 북어, 뽕나무, 헛개나무 및 양파 등을 혼합하여 제조하는 내용도 일부 개시되어 있으나, 종래의 양념장은 대부분 물을 혼합하여 제조할 뿐 뽕잎, 여주, 새싹보리 추출액 등을 담금수로 사용하거나 담금수를 이용하여 제조한 저염 장아찌에 대한 내용은 개시되어 있지 않다.
In the manufacturing process of the general pickled food as shown in Figure 1, by making pepper sauce, red pepper paste, soy sauce, sugar, starch syrup and water to make a marinade, or kelp, anchovies, drumfish, mulberry, walnut tree and onion, etc. Although some of the contents are also disclosed, the conventional seasoning sauce is prepared by mixing mostly water, mulberry leaf, bitter gourd, sprout barley extract, etc. The contents of the low salt pickles prepared using immersion water or immersion water is disclosed. Not.

본 발명은 상기한 바와 같은 종래기술이 가지는 문제를 해결하기 위해 안출된 것으로, 그 목적은 항당뇨에 도움을 주는 뽕잎, 여주 및 새싹보리 추출액을 이용하여 용수를 제조하고, 염도가 낮은(2% 미만) 절임식품 예로, 저염장아찌 및 저염피클을 제조하여 관능적 품질과 저장성이 우수한 절임식품의 제조방법을 제공함에 있다.
The present invention has been made to solve the problems of the prior art as described above, the purpose is to prepare water using mulberry leaves, bitter gourd and sprout barley extract to help anti-diabetes, low salinity (2% Less) pickled foods, for example, to prepare a low pickled pickles and low salt pickles to provide a method for producing pickled foods excellent sensory quality and shelf life.

상기한 바와 같은 본 발명의 기술적 과제는 다음과 같은 수단에 의해 달성되어진다.The technical problem of the present invention as described above is achieved by the following means.

(1) 여주, 뽕잎 및 새싹보리의 군에서 선택된 적어도 1종의 식물추출액을 얻는 단계;(1) obtaining at least one plant extract selected from the group of bitter melon, mulberry leaf and sprout barley;

상기 얻어진 식물추출액을 절임의 대상이 되는 원료에 담금수로 첨가하여 담그는 단계; 및Immersing the obtained plant extract by adding immersion water to a raw material to be pickled; And

상기 담금수에 담근 절임의 대상이 되는 원료를 숙성하여 절임식품을 제조하는 단계;를 포함하는 절임식품의 제조방법.
A method of manufacturing a pickled food, comprising: aging the raw material to be pickled in the immersion water to produce a pickled food.

(2) 상기 (1)에 있어서, 상기 절임식품은 장아찌 혹은 피클인 것을 특징으로 하는 절임식품의 제조방법.
(2) The method of producing pickled food according to the above (1), wherein the pickled food is pickles or pickles.

(3) 상기 (1)에 있어서, 식물추출액은 30~60중량% 첨가되어짐을 특징으로 하는 절임식품의 제조방법.
(3) The method of producing pickled food according to the above (1), wherein the plant extract is added in an amount of 30 to 60% by weight.

(4) 상기 (2)에 있어서, 장아찌는 절임의 대상이 되는 원료에 간장 20~40중량%, 식초 20~30중량%, 설탕 10~20중량%, 및 잔여량의 식물추출액로 이루어진 담금수에 담궈 제조된 것을 특징으로 하는 절임식품의 제조방법.
(4) In the above (2), pickles are immersed in 20 to 40% by weight of soy sauce, 20 to 30% by weight of vinegar, 10 to 20% by weight of sugar, and the remaining amount of plant extracts. Method for producing pickled foods, characterized in that the preparation.

(5) 상기 (2)에 있어서, 피클은 절임의 대상이 되는 원료에 식초 25~40중량%, 설탕 10~25중량%, 및 잔여량의 식물추출액로 이루어진 담금수에 담궈 제조된 것을 특징으로 하는 절임식품의 제조방법.
(5) In the above (2), the pickle is prepared by immersing in immersion water consisting of 25-40% by weight of vinegar, 10-25% by weight of sugar, and the residual amount of plant extract in the raw material to be pickled Method of manufacturing pickled foods.

(6) 상기 (1)에 있어서, 절임의 대상이 되는 원료는 초석잠 뿐만 아니라 돼지감자, 야콘, 도라지, 더덕, 인삼, 오이, 무, 깻잎, 방울양배추, 양파, 마늘, 브로콜리, 취나물, 곰취, 곤드레, 명이나물, 엄나무순, 오가피순, 뽕잎, 두릅, 콩잎, 고구마줄기, 고구마잎, 고구마순, 팽이버섯, 표고버섯, 새송이버섯, 토마토, 밤, 가지, 연근, 고추, 깻잎 및 곤드레의 군에서 선택된 1종인 것을 특징으로 하는 절임식품의 제조방법.
(6) In the above (1), the raw materials to be pickled are not only corner stone but also pork potato, yacon, bellflower, deodeok, ginseng, cucumber, radish, sesame leaf, cabbage, onion, garlic, broccoli, broccoli, bear odor , Gondre, Sprouts, Umbrella, Ogapi, Mulberry, Aralia, Bean Leaf, Sweet Potato Stem, Sweet Potato Leaf, Sweet Potato, Enoki Mushroom, Shiitake, Pleurotus Mushroom, Tomato, Chestnut, Eggplant, Lotus Root, Pepper, Sesame Leaf and Gondre Method for producing pickled foods, characterized in that one selected from the group.

(7) 상기 (1) 내지 (6) 중 선택된 어느 하나의 방법에 의해 제조된 것을 특징으로 하는 절임식품.
(7) Pickled foods, characterized in that produced by any one of the method (1) to (6).

상기와 같이 본 발명에 의하면, 항당뇨에 도움을 주는 뽕잎, 여주 및 새싹보리 추출액을 이용하여 용수를 제조하고, 염도가 낮은(2% 미만) 절임식품 예로, 저염장아찌 및 저염피클을 제조하여 관능적 품질과 저장성이 우수한 절임식품을 제공한다.
According to the present invention as described above, using mulberry leaf, bitter gourd and sprout barley extract to help anti-diabetic, water is prepared, low salt (less than 2%) pickled food example, low salted pickles and low salt pickle Provides pickled foods with excellent quality and shelf life.

도 1은 절임식품의 일반적인 제조공정도이다.1 is a general manufacturing process of the pickled food.

이하, 본 발명의 내용을 보다 상세하게 설명하면 다음과 같다.Hereinafter, the content of the present invention in more detail as follows.

본 발명에 따른 절임식품의 제조방법은 여주, 뽕잎 및 새싹보리의 군에서 선택된 적어도 1종의 식물추출액을 얻는 단계;Method for producing a pickled food according to the present invention comprises the steps of obtaining at least one plant extract selected from the group of Yeoju, mulberry leaves and sprout barley;

상기 얻어진 식물추출액을 절임의 대상이 되는 원료에 담금수로 첨가하여 담그는 단계; 및Immersing the obtained plant extract by adding immersion water to a raw material to be pickled; And

상기 담금수에 담근 절임의 대상이 되는 원료를 숙성하여 절임식품을 제조하는 단계;를 포함한다.It comprises a step of preparing a pickled food by aging the raw material to be pickled in the immersion water.

상기 본 발명에서 담금수에 사용되는 식물추출액은 여주, 뽕잎 및 새싹보리의 군에서 선택된 적어도 1종의 식물이 사용되어진다.Plant extract used in the immersion water in the present invention is used at least one plant selected from the group of Yeoju, mulberry leaves and sprout barley.

본 발명에서는 이들의 열수추출액을 담금수로 이용하며, 바람직하게는 준비된 상기 식물 부피의 10~25배에 해당하는 정제수를 가한 후 70~90℃, 바람직하게는 80℃에서 30분~2시간, 바람직하게는 1시간 동안 쉐이킹해서, 열수 추출한 후, 각 시료를 100 mesh 거름망에 여과하여, 그 상등액을 식물 추출액으로 수득하여 담금수로써 사용한다.In the present invention, these hot water extracts are used as immersion water, and preferably, after adding purified water corresponding to 10 to 25 times the volume of the prepared plant, 70 to 90 ° C, preferably 80 minutes to 30 minutes to 2 hours, Preferably, after shaking for 1 hour, hot water extraction, each sample is filtered through a 100 mesh strainer, and the supernatant is obtained as a plant extract and used as immersion water.

상기 본 발명에서 절임의 대상이 되는 원료는 절임식품으로 통상적으로 섭취되고 있는 다양한 식재료들이 사용될 수 있어 특별한 한정을 요하지는 않으며, 다만 본 발명에서 바람직하게는 초석잠, 돼지감자, 야콘, 도라지, 더덕, 인삼, 오이, 무, 깻잎, 방울양배추, 양파, 마늘, 브로콜리, 취나물, 곰취, 곤드레, 명이나물, 엄나무순, 오가피순, 뽕잎, 두릅, 콩잎, 고구마줄기, 고구마잎, 고구마순, 팽이버섯, 표고버섯, 새송이버섯, 토마토, 밤, 가지, 연근, 고추, 깻잎 및 곤드레의의 군에서 선택된 1종을 들 수 있다.The raw material to be pickled in the present invention is a variety of ingredients that are commonly ingested as a pickled food can be used does not require a special limitation, but preferably in the present invention, seokseokja, pig potatoes, yacon, bellflower, deodeok , Ginseng, Cucumber, Radish, Sesame leaf, Brussels sprouts, Onion, Garlic, Broccoli, Oysters, Bear, Gondre, Sprouts, Umbrella, Ogapisun, Mulberry leaves, Arbor, Soybean leaves, Sweet potato stem, Sweet potato leaf, Sweet potato sprout, Enoki mushroom , Shiitake mushroom, matsutake mushroom, tomato, chestnut, eggplant, lotus root, red pepper, sesame leaf, and one selected from the group of gondre.

상기 본 발명에서 절임식품은 바람직하게는 장아찌 혹은 피클이다.Pickled food in the present invention is preferably pickles or pickles.

만일, 절임식품이 장아찌인 경우 절임의 대상이 되는 원료에 바람직하게는 간장 20~40중량%, 식초 20~30중량%, 설탕 10~20중량%, 및 잔여량의 식물추출액으로 이루어진 담금수가 이용될 수 있다.If the pickled food is pickled pickles, the soaked water consisting of 20 to 40% by weight of soy sauce, 20 to 30% by weight of vinegar, 10 to 20% by weight of sugar, and the residual amount of plant extract may be used as the raw material for pickling Can be.

이와는 달리 절임식품이 피클인 경우, 바람직하게는 절임의 대상이 되는 원료에 바람직하게는 식초 25~40중량%, 설탕 10~25중량%, 및 잔여량의 식물추출액로 이루어진 담금수가 이용될 수 있다.On the contrary, when the pickled food is a pickle, preferably immersion water consisting of 25-40% by weight of vinegar, 10-25% by weight of sugar, and the residual amount of plant extract may be used as a raw material to be pickled.

본 발명에서 절임의 대상이 되는 원료에 담금수로 첨가하여 담그는 과정에서, 세척된 절임의 대상이 되는 원료가 들어 있는 절임통에 담금수(혹은 절임액)를 바람직하게는 원료 부피의 1~3배 정도를 넣는다. In the present invention, in the process of immersion by adding to the raw material to be pickled as immersion water, immersion water (or pickling liquid) in the pickling container containing the raw material to be washed pickled (or pickling solution) is preferably 1 to 3 Put about twice as much.

이후, 절임통에서 절임하는 과정이 진행되며, 바람직하게는 절임 시 원료가 뜨지 않도록 탱크의 상부에 부식성이 없는 재질로 눌러 5~10℃의 온도에서 10~20일간 저장 숙성시킨 다음 실온에서 10일간 후숙시키는 과정을 진행한다.
Thereafter, the process of pickling in a pickling container is carried out, and preferably, the raw material does not float during pickling by pressing with a non-corrosive material on the top of the tank for 10-20 days at a temperature of 5-10 ° C., then aging for 10 days at room temperature. Go through the process of ripening.

이하, 본 발명의 내용을 실험예 및 실시예를 이용하여 상세하게 설명하고자 하나, 이는 본 발명의 이해를 위해 제시되어지는 것일 뿐 본 발명의 권리범위가 이들 실험예 및 실시예로 한정되는 것으로 해석되어질 수 없음은 당연하다.
Hereinafter, the contents of the present invention will be described in detail by using experimental examples and examples, which are presented for the understanding of the present invention, but the scope of the present invention is interpreted to be limited to these experimental examples and examples. Of course it can not be done.

[실시예 1] 여주추출액의 제조방법Example 1 Preparation of Litchi Extract

여주를 세척하고, 건조 및 분쇄하여 준비하였다. 그리고, 준비된 여주 부피의 10배에 해당하는 정제수를 가한 후 80℃에서 1시간 동안 쉐이킹해서, 열수 추출한 후, 각 시료를 100 mesh 거름망에 여과하여, 그 상등액을 여주 추출액로 수득하였다.
The litchi was washed, dried and ground to prepare. Then, after adding purified water corresponding to 10 times the volume of the prepared gourd, shaking for 1 hour at 80 ° C., extracting hot water, and filtering each sample through a 100 mesh sieve, the supernatant was obtained as a gourd extract.

[실시예 2] 뽕잎추출액의 제조방법Example 2 Preparation of Mulberry Leaf Extract

뽕잎를 세척하고, 건조 및 분쇄하여 준비하였다. 그리고, 준비된 뽕잎 부피의 10배에 해당하는 정제수를 가한 후 80℃에서 1시간 동안 쉐이킹해서, 열수 추출한 후, 각 시료를 100 mesh 거름망에 여과하여, 그 상등액을 뽕잎 추출액으로 수득하였다.
Mulberry leaves were washed, dried and ground to prepare. Then, after adding purified water corresponding to 10 times the volume of the mulberry leaf prepared, shaking for 1 hour at 80 ℃, hot water extraction, each sample was filtered through a 100 mesh strainer, the supernatant was obtained as a mulberry leaf extract.

[실시예 3] 새싹보리추출액의 제조방법Example 3 Preparation of Sprout Barley Extract

새싹보리를 세척하고, 건조 및 분쇄하여 준비하였다. 그리고, 준비된 새싹보리 부피의 10~25배에 해당하는 정제수를 가한 후 80℃에서 1시간 동안 쉐이킹해서, 열수 추출한 후, 각 시료를 100 mesh 거름망에 여과하여, 그 상등액을 새싹보리 추출액으로 수득하였다.
The barley was washed, dried and ground to prepare. Then, after adding purified water corresponding to 10-25 times the volume of the sprouted barley, shaking for 1 hour at 80 ° C., and extracting hot water, each sample was filtered through a 100 mesh strainer to obtain the supernatant as the sprouted barley extract. .

[실험예 1] 추출액의 항당뇨 활성Experimental Example 1 Antidiabetic Activity of Extract

당뇨병의 치료제 개발을 위한 여러 기전 중 하나인 α-glucosidase 저해제는 소장에 존재하는 이당류의 소화 효소인 α-glucosidase를 억제하여 이당류가 단당류로 분해되는 것을 막아 소장 융털에서의 흡수를 지연시켜 식후 혈당의 급격한 증가를 막아주어 약화된 췌장에서 분비되는 인슐린만으로도 혈당 조절을 가능하게 하는 기전을 이용하는 치료제이다(Adisakwattana S, 2009).Α-glucosidase inhibitor, one of several mechanisms for the development of a therapeutic agent for diabetes, inhibits the digestion of disaccharides in the small intestine, α-glucosidase, which prevents the breakdown of disaccharides into monosaccharides and delays absorption in the small intestine filament. It is a therapeutic agent that uses a mechanism that prevents a sharp increase and enables blood sugar control by only insulin released from the weakened pancreas (Adisakwattana S, 2009).

시료 10 ㎕에 1 unit/㎖ α-glucosidase 30 ㎕을 첨가하여 37℃에서 10분간 반응시켰다. 1 mM p-nitrophenyl α-D-glucopyranoside 30 ㎕을 첨가하여 37℃에서 20분 동안 반응시킨 후, 1M Na2CO3 130 ㎕을 첨가한 후 microplate reader (VERSAmax, Molecular Device, CA, USA)를 이용하여 405 nm에서 흡광도를 측정하였으며, 측정결과 얻어진 α-glucosidase 저해활성을 하기 표 1에 나타내었다.30 μl of 1 unit / ml α-glucosidase was added to 10 μl of the sample and allowed to react at 37 ° C. for 10 minutes. After 30 μl of 1 mM p-nitrophenyl α-D-glucopyranoside was added and reacted at 37 ° C. for 20 minutes, 130 μl of 1M Na 2 CO 3 was added, followed by using a microplate reader (VERSAmax, Molecular Device, CA, USA). The absorbance was measured at 405 nm, and the obtained α-glucosidase inhibitory activity is shown in Table 1 below.

추출액의 α-glucosidase 저해활성Α-glucosidase Inhibitory Activity of Extracts 추출액 농도Extract concentration 여주추출액Yeoju extract 뽕잎추출액Mulberry Leaf Extract 새싹보리추출액Sprout Barley Extract α-glucosidase 저해활성(%)α-glucosidase inhibitory activity (%) 1%One% 10.03±0.0210.03 ± 0.02 25.12±0.3525.12 ± 0.35 11.47±0.7211.47 ± 0.72 0.5%0.5% 6.60±0.356.60 ± 0.35 17.38±0.6017.38 ± 0.60 8.13±0.288.13 ± 0.28 0.25%0.25% 3.78±0.193.78 ± 0.19 10.84±0.0710.84 ± 0.07 4.12±0.044.12 ± 0.04

[실험예 2] 추출액의 폴리페놀 함량 측정Experimental Example 2 Polyphenol Content Measurement of Extract

시료 0.1 ㎖에 folin-ciocalteu reagent를 첨가하여 혼합한 후 microplate reader (VERSAmax, Molecular Device, CA, USA)를 이용하여 760 nm에서 흡광도를 측정하였다. 총 폴리페놀 함량은 chlorogenic acid를 이용하여 작성한 표준곡선으로부터 산출하였으며, 실험결과는 하기 표 2에 나타내었다.After adding and mixing the folin-ciocalteu reagent in 0.1 ml of the sample, the absorbance was measured at 760 nm using a microplate reader (VERSAmax, Molecular Device, CA, USA). Total polyphenol content was calculated from the standard curve prepared using chlorogenic acid, the experimental results are shown in Table 2 below.

추출액의 폴리페놀 함량Polyphenol Content in Extracts 여주추출액Yeoju extract 뽕잎추출액Mulberry Leaf Extract 새싹보리추출액Sprout Barley Extract 폴리페놀 함량(㎍/㎖)Polyphenol Content (µg / mL) 161.95±1.98161.95 ± 1.98 498.87±2.93498.87 ± 2.93 285.00±6.30285.00 ± 6.30

[실험예 3] 추출액의 DPPH radical 소거능 측정Experimental Example 3 Measurement of DPPH Radical Scavenging Capacity of Extract

추출액의 항산화 활성은 DPPH radical 소거능으로 평가하였다. 메탄올에 0.4 mM의 농도로 용해한 DPPH 용액 160 ㎕와 시료 40 ㎕를 첨가하여 암소에서 30분간 방치한 다음 515 nm에서 흡광도를 측정하였다. 양성 대조군으로 ascorbic acid를 사용하였으며, 측정결과는 하기 표 3에 나타내었다. The antioxidant activity of the extract was evaluated by DPPH radical scavenging activity. 160 μl of DPPH solution dissolved in methanol at a concentration of 0.4 mM and 40 μl of sample were added thereto, and left for 30 minutes in the dark. The absorbance was measured at 515 nm. Ascorbic acid was used as a positive control, and the measurement results are shown in Table 3 below.

추출액의 DPPH radical 소거능 DPPH radical scavenging ability of extract 추출액 농도Extract concentration 여주추출액Yeoju extract 뽕잎추출액Mulberry Leaf Extract 새싹보리추출액Sprout Barley Extract DPPH radical 소거능(%)DPPH radical scavenging ability (%) 1%One% 28.61±2.8228.61 ± 2.82 49.32±0.6649.32 ± 0.66 34.27±0.8934.27 ± 0.89 0.5%0.5% 18.23±1.2918.23 ± 1.29 33.19±0.9233.19 ± 0.92 21.32±1.0221.32 ± 1.02 0.25%0.25% 6.18±1.016.18 ± 1.01 20.84±0.2920.84 ± 0.29 13.58±0.0613.58 ± 0.06

[실시예 4] 저염장아찌 제조Example 4 Preparation of Low Salted Pickles

1) 추출액의 제조1) Preparation of Extract

뽕잎, 여주 및 새싹보리를 세척하고, 건조 및 분쇄하여 준비하였다. 그리고, 준비된 뽕잎, 여주 및 새싹보리 부피의 10배에 해당하는 정제수를 가한 후 80℃에서 1시간 동안 쉐이킹해서, 열수 추출한 후, 각 시료를 100 mesh 거름망에 여과하여, 그 상등액을 뽕잎, 여주 및 새싹보리의 추출액으로 수득하였다.
Mulberry leaves, bitter melon and shoot barley were washed, dried and ground to prepare. Then, after adding purified water corresponding to 10 times the volume of mulberry leaves, bitter melon and shoot barley, shaking at 80 ° C. for 1 hour, extracting hot water, and filtering each sample to 100 mesh strainer, the supernatant was added to mulberry leaf, bitter melon and Obtained as an extract of sprout barley.

2) 담금수의 제조2) Preparation of immersion water

저염 장아찌용 담금수는 하기 표 4~6에 나타낸 조성에 따라 뽕잎, 여주 및 새싹보리 추출액, 간장, 식초, 설탕으로 구성하여 제조하였다.Low salt pickled marinated water was prepared by mulberry leaf, Yeoju and Sprout barley extract, soy sauce, vinegar, sugar according to the composition shown in Tables 4-6.

뽕잎추출액 함유 장아찌의 담금수 조성(중량%)Immersion Composition of Pickles with Mulberry Leaf Extract (wt%) 원료Raw material 조성비Creation costs 실시예4-1Example 4-1 실시예4-2Example 4-2 실시예4-3Example 4-3 실시예4-4Example 4-4 간장Soy sauce 4040 3030 2020 2020 식초vinegar 2020 2525 2525 3030 설탕Sugar 2020 1515 1515 1010 뽕잎 추출액Mulberry Leaf Extract 3030 4040 4040 4040 정제수Purified water -- -- -- -- 합계Sum 100100 100100 100100 100100 염도(%)Salinity (%) 2.82.8 2.52.5 2.52.5 1.71.7

여주추출액 함유 장아찌의 담금수 조성(중량%)Immersion Composition of Pickles Containing Yeoju Extract (wt%) 원료Raw material 조성비Creation costs 실시예4-5Example 4-5 실시예4-6Example 4-6 실시예4-7Example 4-7 실시예4-8Example 4-8 간장Soy sauce 4040 3030 2020 2020 식초vinegar 2020 2525 2525 3030 설탕Sugar 2020 1515 1515 1010 여주 추출액Yeoju extract 3030 4040 4040 4040 정제수Purified water -- -- -- -- 합계Sum 100100 100100 100100 100100 염도(%)Salinity (%) 2.82.8 2.52.5 2.52.5 1.81.8

새싹보리추출액 장아찌의 담금수 조성(중량%)Immersion Composition of Sprout Barley Extract (wt%) 원료Raw material 조성비Creation costs 실시예4-9Example 4-9 실시예4-10Example 4-10 실시예4-11Example 4-11 실시예4-12Example 4-12 대조군Control 간장Soy sauce 4040 3030 2020 2020 4040 식초vinegar 2020 2525 2525 3030 2020 설탕Sugar 2020 1515 1515 1010 2020 새싹보리 추출액Sprout Barley Extract 3030 4040 4040 4040 정제수Purified water -- -- -- -- 3030 합계Sum 100100 100100 100100 100100 100100 염도(%)Salinity (%) 2.82.8 2.52.5 2.52.5 1.81.8 3.43.4

3) 장아찌의 숙성3) Aging of Pickles

상기 표 4~6에서와 같이, 담금수의 조성을 달리하여 배합한 것을 90℃에서 5분간 살균하고 60℃까지 냉각하여 담금수로 제조하였다. 각 담금수를 초석잠 부피의 2배로 하여 절임통에 넣고, 절임 시 초석잠이 뜨지 않도록 탱크의 상부에 부식성이 없는 재질로 눌러 5℃의 온도에서 10일간 저장 숙성시킨 다음 실온에서 10일간 후숙시켰다. 대조군은 식물추출액 대신에 통상 장아찌 제조 시 사용하는 정제수로 실험에 제공하였다.
As shown in Tables 4 to 6, the mixture of different immersion water was sterilized at 90 ° C. for 5 minutes and cooled to 60 ° C. to prepare immersion water. Each immersion water was added to the pickling container at twice the volume of salt, and then pressed with a non-corrosive material on the top of the tank so that the salt did not rise during pickling. . The control group was provided to the experiment as purified water used in the manufacture of pickles instead of plant extracts.

[실험예 4] 장아찌의 저장기간에 따른 pH변화Experimental Example 4 pH Change According to Storage Period of Pickles

대조군과 실시예4-1~4, 4-5, 4-9와 같이 제조된 저염 장아찌의 물성변화를 측정하기 위해 저장기간에 따른 pH의 변화를 측정하였다. 저장기간 동안의 실시예의 pH의 변화는 크게 나타나지 않았으나 대조군에서는 저장 28일차부터 pH가 높아지는 것을 확인하였다(표 7).In order to measure the change in physical properties of the low salt pickles prepared in the control and Examples 4-1 to 4, 4-5, 4-9 was measured the pH change according to the storage period. The pH of the Example was not significantly changed during the storage period, but the control group confirmed that the pH increased from the 28th day of storage (Table 7).

구분division 저장기간(일)에 따른 pH변화PH change according to storage period (days) 00 1414 2828 4242 5656 7070 실시예4-1Example 4-1 3.603.60 3.563.56 3.633.63 3.773.77 3.773.77 3.773.77 실시예4-2Example 4-2 3.603.60 3.453.45 3.583.58 3.603.60 3.773.77 3.863.86 실시예4-3Example 4-3 3.603.60 3.453.45 3.563.56 3.733.73 3.863.86 3.773.77 실시예4-4Example 4-4 3.603.60 3.533.53 3.603.60 3.563.56 3.583.58 3.603.60 실시예4-5Example 4-5 3.603.60 3.463.46 3.523.52 3.583.58 3.603.60 3.623.62 실시예4-9Example 4-9 3.603.60 3.453.45 3.543.54 3.613.61 3.623.62 3.703.70 대조군Control 3.553.55 3.563.56 3.733.73 3.863.86 3.773.77 3.853.85

[실험예 5] 장아찌의 저장기간에 따른 미생물(일반세균수) 변화Experimental Example 5 Change of Microorganisms (General Bacterial Count) According to Storage Period of Pickles

대조군과 실시예4-1~4, 4-5, 4-9와 같이 제조된 저염 장아찌의 세균변화를 확인하였다. 반투명한 폴리에틸렌 용기에 넣고 밀봉하여 10℃의 항온기에 넣고 세균수의 변화를 확인하였다. 대조군과 실시예4-1~4, 4-5, 4-9 모두 식품공전상의 절임류의 일반세균 규격은 1.0×105 cfu/g 으로 저장 기간 동안의 일반세균의 변화는 공전 규격상에는 적합함을 확인하였다. 대조군에서는 저장 28일차부터 일반세균수의 증가속도가 높은 것으로 나타났으나, 실시예 4-1~4, 4-5, 4-9의 경우 대조군보다 일반세균수의 변화가 작았다(표 8).Bacterial changes of low salt pickles prepared as controls and Examples 4-1 to 4, 4-5, and 4-9 were confirmed. The container was placed in a translucent polyethylene container, sealed, and placed in a thermostat at 10 ° C. to confirm the change in the number of bacteria. In the control group and in Examples 4-1 to 4, 4-5, and 4-9, the general bacterium specification of the pickles in the food industry was 1.0 × 10 5 cfu / g. Confirmed. In the control group, the increase rate of the general bacterial count was higher from the 28th day of storage, but in Examples 4-1 to 4, 4-5, and 4-9, the change of the general bacterial count was smaller than that of the control group (Table 8). .

구분division 저장기간(일)에 따른 일반세균수 변화Changes in General Bacterial Count According to Storage Period (days) 00 1414 2828 4242 5656 7070 실시예4-1Example 4-1 1.1×102 1.1 × 10 2 1.1×102 1.1 × 10 2 1.2×102 1.2 × 10 2 1.4×102 1.4 × 10 2 2.6×102 2.6 × 10 2 5.3×102 5.3 × 10 2 실시예4-2Example 4-2 1.1×102 1.1 × 10 2 1.1×102 1.1 × 10 2 1.3×102 1.3 × 10 2 1.5×102 1.5 × 10 2 3.5×102 3.5 × 10 2 5.7×102 5.7 × 10 2 실시예4-3Example 4-3 1.1×102 1.1 × 10 2 2.1×102 2.1 × 10 2 2.0×102 2.0 × 10 2 3.8×102 3.8 × 10 2 3.5×102 3.5 × 10 2 6.2×102 6.2 × 10 2 실시예4-4Example 4-4 1.1×102 1.1 × 10 2 1.0×102 1.0 × 10 2 1.0×102 1.0 × 10 2 2.1×102 2.1 × 10 2 5.7×102 5.7 × 10 2 5.3×102 5.3 × 10 2 실시예4-5Example 4-5 1.1×102 1.1 × 10 2 1.1×102 1.1 × 10 2 1.2×102 1.2 × 10 2 1.4×102 1.4 × 10 2 2.5×102 2.5 × 10 2 5.1×102 5.1 × 10 2 실시예4-9 Example 4-9 1.1×102 1.1 × 10 2 1.1×102 1.1 × 10 2 1.2×102 1.2 × 10 2 1.5×102 1.5 × 10 2 3.0×102 3.0 × 10 2 5.6×102 5.6 × 10 2 대조군Control 1.1×102 1.1 × 10 2 1.5×102 1.5 × 10 2 2.7×102 2.7 × 10 2 4.4×102 4.4 × 10 2 6.2×102 6.2 × 10 2 9.2×102 9.2 × 10 2

[실험예 6] 장아찌의 저장기간에 따른 관능검사Experimental Example 6 Sensory Evaluation According to Storage Period of Pickles

본 실시예에 따라 제조된 장아찌를 종래의 저염장아찌와 비교하여 관능평가를 수행하였다. 관능 평가는 맛, 조직감, 짠맛 선호도 및 전체 기호도에 대해 아래의 기준으로 평가하도록 하였다(표 9~11). 평가는 20~40대 20명을 대상으로 이루어졌다.The sensory evaluation was performed by comparing the pickles prepared according to the present example with conventional low salt pickles. Sensory evaluation was to evaluate the taste, texture, salty preference and overall preference based on the following criteria (Table 9-11). The evaluation was conducted on 20 people in their 20s to 40s.

[점수 기준] 9~7점: 매우 우수~우수, 6~4점: 보통, 3~1점: 나쁨~매우나쁨[Score] 9 ~ 7 points: Very good ~ Excellent, 6 ~ 4 points: Normal, 3 ~ 1 points: Bad ~ Very bad

구분division 실시예4-1Example 4-1 실시예4-2Example 4-2 실시예4-3Example 4-3 실시예4-4Example 4-4 flavor 7.57.5 7.07.0 8.28.2 8.38.3 조직감Organization 8.58.5 8.08.0 8.08.0 8.58.5 짠맛 선호도Salty Preference 6.06.0 6.56.5 7.07.0 9.09.0 전체 기호도Full symbol 7.57.5 7.07.0 8.28.2 8.58.5

구분division 실시예4-5Example 4-5 실시예4-6Example 4-6 실시예4-7Example 4-7 실시예4-8Example 4-8 flavor 7.47.4 7.17.1 8.18.1 8.28.2 조직감Organization 8.48.4 8.08.0 8.18.1 8.68.6 짠맛 선호도Salty Preference 6.16.1 6.46.4 7.07.0 9.19.1 전체 기호도Full symbol 7.47.4 7.17.1 8.18.1 8.68.6

구분division 실시예
4-9
Example
4-9
실시예
4-10
Example
4-10
실시예
4-11
Example
4-11
실시예
4-12
Example
4-12
A사제품A company product 대조군Control
flavor 7.47.4 7.27.2 7.67.6 8.28.2 8.58.5 7.07.0 조직감Organization 8.68.6 8.38.3 8.18.1 8.18.1 8.08.0 8.58.5 짠맛 선호도Salty Preference 6.16.1 6.46.4 7.07.0 9.19.1 6.56.5 7.07.0 전체 기호도Full symbol 7.37.3 7.27.2 8.18.1 8.28.2 8.08.0 7.07.0

맛, 조직감, 전체 기호도에서는 큰 차이를 보이지 않았으나 짠맛 선호도에서 종래의 제품과 대조군보다 높은 선호도가 나타남을 확인할 수 있었다. 이에 본 발명에 따르면 항당뇨(α-glucosidase 저해)활성 및 항산화활성이 높은 뽕잎추출액을 사용함으로써 기능성뿐만 아니라 염도를 낮춰 기호성이 강화된 저염장아찌를 제조할 수 있다.
Taste, texture, overall preference did not show a significant difference, but the salty preference was found to be higher preference than the conventional product and the control group. Therefore, according to the present invention, by using a mulberry leaf extract with high anti-diabetic (α-glucosidase inhibition) activity and antioxidant activity, low salted pickles with enhanced palatability can be prepared by lowering salinity as well as functionality.

[실시예 5] 저염피클 제조Example 5 Preparation of Low Salt Pickles

1) 추출액의 제조1) Preparation of Extract

뽕잎, 여주 및 새싹보리를 세척하고, 건조 및 분쇄하여 준비하였다. 그리고, 준비된 뽕잎, 여주 및 새싹보리 부피의 10배에 해당하는 정제수를 가한 후 80℃에서 1시간 동안 쉐이킹해서, 열수 추출한 후, 각 시료를 100 mesh 거름망에 여과하여, 그 상등액을 뽕잎, 여주 및 새싹보리의 추출액로 수득하였다.
Mulberry leaves, bitter melon and shoot barley were washed, dried and ground to prepare. Then, after adding purified water corresponding to 10 times the volume of mulberry leaves, bitter melon and shoot barley, shaking at 80 ° C. for 1 hour, extracting hot water, and filtering each sample to 100 mesh strainer, the supernatant was added to mulberry leaf, bitter melon and Obtained as an extract of sprout barley.

2) 담금수의 제조2) Preparation of immersion water

저염 피클용 담금수는 하기 표 12~14에 나타낸 조성에 따라 뽕잎, 여주 및 새싹보리 추출액, 식초, 설탕으로 구성하여 제조하였다.Low salt pickle immersion water was prepared by consisting of mulberry leaves, bitter gourd and Sprout barley extract, vinegar, sugar according to the composition shown in Tables 12-14.

뽕잎추출액 피클의 담금수 조성(중량%)Immersion Composition of Mulberry Leaf Extract Pickle (wt%) 원료Raw material 조성비Creation costs 실시예5-1Example 5-1 실시예5-2Example 5-2 실시예5-3Example 5-3 실시예5-4Example 5-4 식초vinegar 2525 2525 3030 4040 설탕Sugar 2525 1515 1515 1010 뽕잎 추출액Mulberry Leaf Extract 5050 6060 5555 5050 정제수Purified water -- -- -- -- 합계Sum 100100 100100 100100 100100

여주추출액 피클의 담금수 조성(중량%)Dipping Water Composition of Yeoju Extract Pickle (wt%) 원료Raw material 조성비Creation costs 실시예5-5Example 5-5 실시예5-6Example 5-6 실시예5-7Example 5-7 실시예5-8Example 5-8 식초vinegar 2525 2525 3030 4040 설탕Sugar 2525 1515 1515 1010 여주 추출액Yeoju extract 5050 6060 5555 5050 정제수Purified water -- -- -- -- 합계Sum 100100 100100 100100 100100

새싹보리추출액 피클의 담금수 조성(중량%)Immersion Composition of Sprout Barley Extract Pickle (wt%) 원료Raw material 조성비Creation costs 실시예5-9Example 5-9 실시예5-10Example 5-10 실시예5-11Example 5-11 실시예5-12Example 5-12 대조군Control 식초vinegar 2525 2525 3030 4040 2525 설탕Sugar 2525 1515 1515 1010 2525 새싹보리추출액Sprout Barley Extract 5050 6060 5555 5050 정제수Purified water -- -- -- -- 5050 합계Sum 100100 100100 100100 100100 100100

3) 피클의 숙성3) pickling ripening

상기 표 12~14에서와 같이, 담금수의 조성을 달리하여 배합한 것을 90℃에서 5분간 살균하고 60℃까지 냉각하여 담금수로 제조하였다. 각 담금수를 초석잠 부피의 2배로 하여 절임통에 넣고, 절임 시 초석잠이 뜨지 않도록 탱크의 상부에 부식성이 없는 재질로 눌러 5℃의 온도에서 10일간 저장 숙성시킨 다음 실온에서 10일간 후숙시켰다. 대조군은 추출액 대신에 통상 장아찌 제조 시 사용하는 정제수로 실험에 제공하였다.
As shown in Tables 12 to 14, the mixture of different immersion water was sterilized at 90 ° C. for 5 minutes and cooled to 60 ° C. to prepare immersion water. Each immersion water was added to the pickling container at twice the volume of salt, and then pressed with a non-corrosive material on the top of the tank so that the salt did not rise during pickling. . The control group was used in the experiment with purified water used for preparing pickles instead of extracts.

[실험예 7] 피클의 저장기간에 따른 pH변화Experimental Example 7 pH Change According to Storage Period of Pickles

대조군과 실시예 5-1~4, 5-5, 5-9와 같이 제조된 저염 피클의 물성변화를 측정하기 위해 저장기간에 따른 pH의 변화를 측정하였다. 저장기간 동안의 실시예의 pH의 변화는 크게 나타나지 않았으나 대조군에서는 저장 70일차부터 pH가 높아지는 것을 확인하였다(표 15).In order to measure the change in physical properties of the low salt pickle prepared as a control and Examples 5-1 to 4, 5-5, 5-9, the change in pH according to the storage period was measured. The pH of the Example was not significantly changed during the storage period, but the control group confirmed that the pH increased from the 70th day of storage (Table 15).

구분division 저장기간(일)에 따른 pH변화PH change according to storage period (days) 00 1414 2828 4242 5656 7070 실시예5-1Example 5-1 3.203.20 3.363.36 3.303.30 3.253.25 3.253.25 3.173.17 실시예5-2Example 5-2 3.203.20 3.203.20 3.283.28 3.103.10 3.223.22 3.243.24 실시예5-3Example 5-3 3.203.20 3.253.25 3.313.31 3.173.17 3.233.23 3.323.32 실시예5-4Example 5-4 3.203.20 3.233.23 3.203.20 3.253.25 3.313.31 3.423.42 실시예5-5 Example 5-5 3.203.20 3.223.22 3.283.28 3.133.13 3.243.24 3.253.25 실시예5-9Example 5-9 3.203.20 3.213.21 3.263.26 3.113.11 3.223.22 3.243.24 대조군Control 3.153.15 3.243.24 3.273.27 3.263.26 3.373.37 3.503.50

[실험예 8] 피클의 저장기간에 따른 미생물(일반 세균수)변화[Experimental Example 8] Change of microorganisms (general bacteria) according to the storage period of the pickle

대조군과 실시예 5-1~4, 5-5, 5-9와 같이 제조된 저염 피클의 세균변화를 확인하였다. 반투명한 폴리에틸렌 용기에 넣고 밀봉하여 10℃의 항온기에 넣고 세균수의 변화를 확인하였다(표 10). 대조군과 실시예 5-1~4, 5-5, 5-9 모두 식품공전상의 절임류의 일반세균 규격은 1.0×105 cfu/g 으로 저장 기간 동안의 일반세균의 변화는 공전 규격상에는 적합함을 확인하였다. 대조군에서는 저장 14일차부터 일반세균수가 비교대상 실시예의 경우보다 높은 것으로 나타났다.Bacterial changes of the low salt pickle prepared as a control and Examples 5-1 to 4, 5-5 and 5-9 were confirmed. It was placed in a translucent polyethylene container, sealed and placed in a thermostat at 10 ° C. to confirm the change in the number of bacteria (Table 10). In the control group and in Examples 5-1 to 4, 5-5, and 5-9, the general bacterium specification of the pickles in the food industry was 1.0 × 10 5 cfu / g. Confirmed. In the control group, the general bacterial count was higher than that of the comparative example from the 14th day of storage.

구분division 저장기간(일)에 따른 일반세균수 변화Changes in General Bacterial Count According to Storage Period (days) 00 1414 2828 4242 5656 7070 실시예5-1Example 5-1 1.0×101 1.0 × 10 1 2.5×101 2.5 × 10 1 3.0×101 3.0 × 10 1 1.0×102 1.0 × 10 2 3.5×102 3.5 × 10 2 3.4×102 3.4 × 10 2 실시예5-2Example 5-2 1.0×101 1.0 × 10 1 2.5×101 2.5 × 10 1 3.0×101 3.0 × 10 1 2.0×102 2.0 × 10 2 5.5×102 5.5 × 10 2 9.4×102 9.4 × 10 2 실시예5-3Example 5-3 1.0×101 1.0 × 10 1 2.5×101 2.5 × 10 1 1.3×101 1.3 × 10 1 1.6×102 1.6 × 10 2 3.9×102 3.9 × 10 2 4.3×102 4.3 × 10 2 실시예5-4Example 5-4 1.0×101 1.0 × 10 1 2.3×101 2.3 × 10 1 2.0×101 2.0 × 10 1 3.5×101 3.5 × 10 1 6.5×101 6.5 × 10 1 1.2×102 1.2 × 10 2 실시예5-5Example 5-5 1.0×101 1.0 × 10 1 2.4×101 2.4 × 10 1 2.2×101 2.2 × 10 1 2.2×102 2.2 × 10 2 3.8×102 3.8 × 10 2 4.0×102 4.0 × 10 2 실시예5-9Example 5-9 1.0×101 1.0 × 10 1 2.3×101 2.3 × 10 1 3.0×101 3.0 × 10 1 2.5×102 2.5 × 10 2 4.2×102 4.2 × 10 2 2.9×102 2.9 × 10 2 대조군Control 1.2×101 1.2 × 10 1 5.3×101 5.3 × 10 1 6.4×101 6.4 × 10 1 5.6×102 5.6 × 10 2 7.9×102 7.9 × 10 2 6.3×102 6.3 × 10 2

[실험예 9] 피클의 저장기간에 따른 관능검사Experimental Example 9 Sensory Evaluation According to Storage Period of Pickles

본 실시예에 따라 제조된 피클을 종래의 저염 피클과 비교하여 관능평가를 수행하였다. 관능 평가는 맛, 조직감, 짠맛 선호도 및 전체 기호도에 대해 아래의 기준으로 평가하도록 하였다(표 17~19). 평가는 20~40대 20명을 대상으로 이루어졌다.Sensory evaluation was performed by comparing the pickles prepared according to the present example with conventional low salt pickles. Sensory evaluation was to evaluate the taste, texture, salty preference and overall preference based on the following criteria (Table 17-19). The evaluation was conducted on 20 people in their 20s to 40s.

[점수 기준] 9~7점: 매우 우수~우수, 6~4점: 보통, 3~1점: 나쁨~매우나쁨[Score] 9 ~ 7 points: Very good ~ Excellent, 6 ~ 4 points: Normal, 3 ~ 1 points: Bad ~ Very bad

구분division 실시예5-1Example 5-1 실시예5-2Example 5-2 실시예5-3Example 5-3 실시예5-4Example 5-4 flavor 8.48.4 8.38.3 8.08.0 8.78.7 조직감Organization 9.19.1 8.28.2 7.67.6 8.68.6 짠맛 선호도Salty Preference 7.67.6 8.18.1 8.28.2 9.19.1 전체 기호도Full symbol 8.18.1 7.87.8 8.28.2 8.88.8

구분division 실시예5-5Example 5-5 실시예5-6Example 5-6 실시예5-7Example 5-7 실시예5-8Example 5-8 flavor 8.58.5 8.58.5 8.28.2 8.58.5 조직감Organization 9.09.0 8.58.5 8.08.0 8.58.5 짠맛 선호도Salty Preference 7.57.5 7.07.0 8.08.0 9.09.0 전체 기호도Full symbol 8.08.0 7.57.5 8.28.2 8.78.7

구분division 실시예5-9Example 5-9 실시예5-10Example 5-10 실시예5-11Example 5-11 실시예5-12Example 5-12 B사제품Company B 대조군Control flavor 8.48.4 8.68.6 8.48.4 8.58.5 8.58.5 8.08.0 조직감Organization 9.09.0 8.68.6 8.18.1 8.28.2 8.08.0 8.08.0 짠맛 선호도Salty Preference 7.57.5 7.57.5 8.18.1 8.88.8 7.07.0 6.56.5 전체 기호도Full symbol 8.08.0 8.68.6 8.38.3 8.68.6 8.58.5 8.18.1

상기 결과에서 나타난 바와 같이, 맛, 조직감에서는 큰 차이를 보이지 않았으나 짠맛 선호도 및 전체 기호도에서 종래의 제품과 대조군에서보다 높은 선호도가 나타남을 확인할 수 있었다.
As shown in the above results, the taste and texture did not show a big difference, but the salty preference and overall preference was found to be higher preference than the conventional products and the control group.

상기와 같이 본 발명에 따르면 항당뇨(α-glucosidase 저해)활성 및 항산화활성이 높은 식물추출액을 사용함으로서 기능성뿐만 아니라 염도를 낮춰 기호성이 강화된 저염장아찌 및 저염피클을 제조할 수 있어 절임용 식품에 담금수로서 매우 적합함을 확인할 수 있었다.
According to the present invention as described above, by using a plant extract with high anti-diabetic (α-glucosidase) activity and antioxidant activity, it is possible to produce low salt pickles and low salt pickles with enhanced palatability by lowering salinity as well as functionality, and to pickling foods. It was confirmed that it is very suitable as the immersion water.

[실시예 6] 저염장아찌 제조Example 6 Preparation of Low Salted Pickles

초석잠 대신 돼지감자를 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that porcine potatoes were used instead of salty nap.

[실시예 7] 저염장아찌 제조Example 7 Preparation of Low Salted Pickles

초석잠 대신 무를 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as in Example 4-1, except that radish was used instead of saltpeter.

[실시예 8] 저염장아찌 제조Example 8 Preparation of Low Salted Pickles

초석잠 대신 토마토를 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that tomatoes were used instead of saltpeter.

[실시예 9] 저염장아찌 제조Example 9 Preparation of Low Salted Pickles

초석잠 대신 밤을 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that chestnuts were used instead of saltpeter.

[실시예 10] 저염장아찌 제조Example 10 Preparation of Low Salted Pickles

초석잠 대신 가지를 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that branches were used instead of corner salt.

[실시예 11] 저염장아찌 제조Example 11 Preparation of Low Salted Pickles

초석잠 대신 연근을 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that lotus root was used instead of cornerstone.

[실시예 12] 저염장아찌 제조Example 12 Preparation of Low Salted Pickles

초석잠 대신 고추를 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that red pepper was used instead of saltpeter.

[실시예 13] 저염장아찌 제조Example 13 Preparation of Low Salted Pickles

초석잠 대신 깻잎을 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that sesame leaves were used instead of salt leaf.

[실시예 14] 저염장아찌 제조Example 14 Manufacture of Low Salted Pickles

초석잠 대신 우엉을 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that burdock was used instead of salt cake.

[실시예 15] 저염장아찌 제조Example 15 Preparation of Low Salted Pickles

초석잠 대신 콩잎을 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as in Example 4-1, except that the soybean leaves were used instead of the salty nap.

[실시예 16] 저염장아찌 제조Example 16 Preparation of Low Salted Pickles

초석잠 대신 곤드레를 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that Gondre was used instead of corner salt.

[실시예 17] 저염장아찌 제조Example 17 Preparation of Low Salted Pickles

초석잠 대신 고구마줄기를 이용한 것을 제외하고는 실시예 4-1의 것과 동일한 조건에 따라 장아찌를 제조하였다.
Pickles were prepared according to the same conditions as those of Example 4-1, except that sweet potato stems were used instead of corner salt.

[실시예 18] 저염피클 제조Example 18 Preparation of Low Salt Pickles

초석잠 대신 돼지감자를 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as those of Example 5-1, except that porcine potatoes were used instead of saltbough.

[실시예 19] 저염피클 제조Example 19 Preparation of Low Salt Pickles

초석잠 대신 무를 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as in Example 5-1, except that radish was used instead of saltpeter.

[실시예 20] 저염피클 제조Example 20 Preparation of Low Salt Pickles

초석잠 대신 토마토를 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as those of Example 5-1, except that tomatoes were used instead of salty nap.

[실시예 21] 저염피클 제조Example 21 Preparation of Low Salt Pickles

초석잠 대신 밤을 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as those of Example 5-1, except that chestnuts were used instead of salty nap.

[실시예 21] 저염피클 제조Example 21 Preparation of Low Salt Pickles

초석잠 대신 가지를 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as those of Example 5-1, except that branches were used instead of cornerstone.

[실시예 22] 저염피클 제조Example 22 Preparation of Low Salt Pickles

초석잠 대신 연근을 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as those of Example 5-1, except that lotus root was used instead of cornerstone.

[실시예 23] 저염피클 제조Example 23 Preparation of Low Salt Pickles

초석잠 대신 고추를 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as those of Example 5-1, except that pepper was used instead of saltpeter.

[실시예 24] 저염피클 제조Example 24 Preparation of Low Salt Pickles

초석잠 대신 깻잎을 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as in Example 5-1, except that sesame leaves were used instead of salt leaf.

[실시예 25] 저염피클 제조Example 25 Preparation of Low Salt Pickles

초석잠 대신 우엉을 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as those of Example 5-1, except that burdock was used instead of salty nap.

[실시예 26] 저염피클 제조Example 26 Preparation of Low Salt Pickles

초석잠 대신 콩잎을 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
Pickles were prepared according to the same conditions as those of Example 5-1 except for using soybean leaves instead of saltpeter.

[실시예 27] 저염피클 제조Example 27 Preparation of Low Salt Pickles

초석잠 대신 곤드레를 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.
A pickle was prepared according to the same conditions as in Example 5-1 except that Gondre was used instead of the cornerstone.

[실시예 28] 저염피클 제조Example 28 Preparation of Low Salt Pickles

초석잠 대신 고구마줄기를 이용한 것을 제외하고는 실시예 5-1의 것과 동일한 조건에 따라 피클을 제조하였다.Pickles were prepared according to the same conditions as those of Example 5-1, except that sweet potato stems were used instead of the salty nap.

상기와 같이, 본 발명의 바람직한 실시 예를 참조하여 설명하였지만 해당 기술 분야의 숙련된 당업자라면 하기의 특허청구범위에 기재된 본 발명의 사상 및 영역으로부터 벗어나지 않는 범위 내에서 본 발명을 다양하게 수정 및 변경시킬 수 있음을 이해할 수 있을 것이다.
As described above, it has been described with reference to a preferred embodiment of the present invention, but those skilled in the art various modifications and changes of the present invention without departing from the spirit and scope of the invention described in the claims below I can understand that you can.

Claims (7)

삭제delete 삭제delete 삭제delete 삭제delete 새싹보리의 부피의 10~25배에 해당하는 정제수를 가하여 70~90℃, 30분~2시간 동안 쉐이킹하여 열수추출하는 단계;
열수추출된 새싹보리의 추출액을 100mesh 거름망에 여과하여 그 상등액을 얻는 단계;
절임의 대상이 되는 원료가 들어있는 절임통에 식초 25~40중량%, 설탕 10~25중량%, 및 잔여량의 상기 상등액으로 이루어진 담금수를 상기 원료 부피의 1~3배로 하여 담그는 단계; 및
원료가 뜨지 않도록 절임통의 상부에 부식성이 없는 재질로 눌러 5~10℃ 온도에서 10~20일간 숙성하고, 실온에서 10일간 후숙시켜 피클을 제조하는 단계;를 포함하는 절임식품의 제조방법.
Adding purified water corresponding to 10 to 25 times the volume of sprout barley and shaking for 70 to 90 ° C. for 30 minutes to 2 hours to extract hot water;
Filtering the extract of hydrothermally extracted bud barley into a 100mesh sieve to obtain the supernatant;
Dipping immersion water consisting of 25-40% by weight of vinegar, 10-25% by weight of sugar, and the remaining amount of the supernatant in a pickling container containing the raw material to be picked 1 to 3 times the volume of the raw material; And
Pressing with a non-corrosive material on the top of the pickles so that the raw material does not float, aged for 10 to 20 days at a temperature of 5 ~ 10 ℃, ripening at room temperature for 10 days to prepare a pickle; manufacturing method of pickled foods comprising a.
제 5항에 있어서, 절임의 대상이 되는 원료는 초석잠, 돼지감자, 야콘, 도라지, 더덕, 인삼, 오이, 무, 깻잎, 방울양배추, 양파, 마늘, 브로콜리, 취나물, 곰취, 곤드레, 명이나물, 엄나무순, 오가피순, 뽕잎, 두릅, 콩잎, 고구마줄기, 고구마잎, 고구마순, 팽이버섯, 표고버섯, 새송이버섯, 토마토, 밤, 가지, 연근, 고추, 깻잎 및 곤드레의 군에서 선택된 1종인 것을 특징으로 하는 절임식품의 제조방법.According to claim 5, The raw materials for pickling are: Choseok nap, pork potato, yacon, bellflower, reddock, ginseng, cucumber, radish, sesame leaf, cabbage, onion, garlic, broccoli, broth, gom, gondre, famous 1 species selected from the group of, Umbrella, Ogapi, Mulberry, Aralia, Soybean Leaf, Sweet Potato Stem, Sweet Potato Leaf, Sweet Potato Shoot, Enoki Mushroom, Shiitake Mushroom, Pleurotus Mushroom, Tomato, Chestnut, Eggplant, Lotus Root, Pepper, Sesame Leaf and Gondre Method for producing a pickled food, characterized in that. 제 5항의 방법에 의해 제조된 것을 특징으로 하는 절임식품.
Pickled foods prepared by the method of claim 5.
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