KR102023724B1 - Skin-moisturizing hydrogel capsules and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a hydrogel capsule for skin moisturizing and a method of manufacturing the same. Specifically, it contains a mixture of colostrum derived from colostrum excellent in moisturizing skin and a fat soluble component having skin moisturizing property in one hydrogel capsule, and delivers water-soluble protein and fat soluble component at the same time when applied to the skin. It relates to a hydrogel capsule and a method for producing the same that can enhance the efficacy and increase the efficiency absorbed by the skin.

Description

Hydrogel capsule for skin moisturizing and its manufacturing method {SKIN-MOISTURIZING HYDROGEL CAPSULES AND MANUFACTURING METHOD THEREOF}

The present invention relates to a hydrogel capsule for skin moisturizing and a method of manufacturing the same. Specifically, it contains a mixture of colostrum derived from colostrum excellent in moisturizing skin and a fat soluble component having skin moisturizing property in one hydrogel capsule, and delivers water-soluble protein and fat soluble component at the same time when applied to the skin. It relates to a hydrogel capsule and a method for producing the same that can enhance the efficacy and increase the efficiency absorbed by the skin.

The skin is divided into three layers of epidermis, dermis and subcutaneous tissue in order from the outside and protects the organs of the body from external stimuli such as changes in temperature and humidity, ultraviolet rays, air pollutants and chemicals. In particular, the skin plays an important role in controlling the evaporation of about 70% of the water in the body. Moisture carries a variety of biologically active substances necessary for the human body and helps keep the skin soft and moist.

The epidermis is separated from the outside in the order of the stratum corneum, granule layer, pole layer, basal layer, the cells of the stratum corneum acts like a brick, and the intercellular lipids between the stratum corneum acts like a mortar to form the skin barrier (J Invest.Dermatol. 80 (Suppl.), Pp. 44-49. 1983. In general, keratinocytes distributed in the human epidermis have high concentrations of natural moisturizing factors and help the skin retain moisture. However, due to factors such as living environment, stress, environmental pollution, aging, etc., the moisture in the stratum corneum decreases, resulting in the drying of the skin and the roughening of the skin. do.

Conventional moisturizers have a function of absorbing moisture or preventing moisture from evaporating from the skin and thus increasing moisture retention in the stratum corneum. However, the moisturizer does not maintain long-term continuous moisturizing performance and is inconvenient to use due to heterogeneity in the skin. Has been.

Meanwhile, as the frequency of various environmental skin diseases such as atopic dermatitis has recently increased, various skin-related products including skin moisturizers are required to cure and improve skin diseases as well as continuous moisturizing. Korean Patent Registration No. 10-0404072 discloses a method of applying a lipid complex or a steroid preparation for moisturizing and restoring the skin barrier, but its use is limited due to problems such as deterioration of skin safety and side effects.

In addition, such skin-related products for moisturizing and improving skin diseases have been developed in a variety of formulations, but the effect is different depending on the formulation, the effect is limited to the realization of the temporary and continuous performance of the improvement efficiency for the skin It has a low problem.

Therefore, there is a need for research and development of a carrier capable of long-term continuous skin care as well as improving the absorption rate of the active ingredient delivered to the skin while having excellent skin moisturizing and improving performance.

Korean Patent Publication No. 10-0404072 (2003.10.21)

An object of the present invention is to provide a hydrogel capsule containing a colostrum-derived protein mixture excellent in moisturizing skin and a fat-soluble component having skin moisturizing property in one hydrogel capsule.

Another object of the present invention is to provide a hydrogel capsule that effectively blocks off-odor present in colostrum-derived protein mixture, stably protects the protein mixture from various oxidative environments of oil and water phases, and suppresses denaturation. do.

It is another object of the present invention to provide a hydrogel capsule which contains an active ingredient that is effective in moisturizing the complex but is stable in the cosmetic formulation, and which can promote skin absorption efficiency and obtain a synergistic effect in moisturizing the skin. The purpose.

Another object of the present invention is to provide a hydrogel capsule containing water-soluble protein and a fat-soluble component in one hydrogel capsule, and at the same time by delivering a water-soluble protein and a fat-soluble component at the time of skin application to balance the oil and moisture. .

Another object of the present invention is to provide a method for producing a hydrogel capsule that can continuously produce a hydrogel capsule in water-in-oil-in-water form while having uniform physical properties.

One aspect of the present invention for achieving the above object is an aqueous phase comprising a protein mixture and a water-soluble polymer derived from colostrum; A fat-soluble intermediate layer located on the water core; It relates to a hydrogel capsule for skin moisturizing comprising; and a polymer hydrogel capsule layer located on the intermediate layer.

According to one aspect of the invention, the water-soluble polymer of the water phase core may include a water-soluble polysaccharide or a water-soluble synthetic polymer.

According to an aspect of the present invention, the fat-soluble intermediate layer may include one or two or more selected from unsaturated fatty acids, tocopheryl oils and vegetable oils.

According to an aspect of the present invention, the polymer hydrogel capsule layer may include a water-soluble polysaccharide.

According to one aspect of the present invention, the water-soluble polysaccharide may be one containing a carboxylic acid group-containing polysaccharide.

According to one aspect of the invention, the polymer hydrogel capsule layer may be to include a cross-linked water-soluble polysaccharide. According to one aspect of the invention, the polymer hydrogel capsule layer may further comprise a water-soluble polysaccharide substituted with a hydrophobic group.

According to one aspect of the invention, the diameter ratio of the water core and the hydrogel capsule may be 1: 1.5 to 1: 15.

According to one aspect of the invention, the aqueous core may further comprise an spirulina extract.

Another aspect of the present invention,

A water-in-oil emulsion forming step in which an aqueous phase droplet containing a protein mixture derived from colostrum and a water-soluble polymer forms an inner phase, and an oil phase containing a fat-soluble component forms an outer phase;

A dispersed phase injection step of injecting the water-in-oil emulsion through a dispersed phase injection hole;

A continuous phase injection step of injecting a continuous phase containing a water-soluble polysaccharide into an induction pipe communicating with the dispersion phase inlet port at an acute angle to the perpendicular direction at the same time as the dispersed phase injection step;

Discharging the water-in-oil emulsion through the continuous phase through a discharge port located opposite the injection direction of the dispersed phase to form an oil-in-water emulsion; And

It relates to a method for producing a hydrogel capsule comprising; encapsulating the discharged water-in-oil emulsion.

In the method for producing a hydrogel capsule according to an aspect of the present invention, the water-soluble polysaccharide of the continuous phase injection step may include a carboxylic acid group-containing polysaccharide.

In the method for producing a hydrogel capsule according to an aspect of the present invention, the encapsulation step may be to encapsulate the water-in-oil-in-water emulsion is crosslinked in an oil bath containing a crosslinking agent.

In the method for producing a hydrogel capsule according to an aspect of the present invention, the crosslinking agent may be a water-soluble salt containing calcium ions.

In the method of manufacturing a hydrogel capsule according to an aspect of the present invention, the continuous phase may further include hyaluronic acid substituted with a hydrophobic group or pectin substituted with a hydrophobic group.

In addition, another aspect of the present invention relates to a hydrogel capsule produced by the above production method.

Hydrogel capsules for skin moisturizing according to the present invention has the advantage of stably collecting the protein mixture and fat-soluble components derived from colostrum as an active ingredient in one hydrogel capsule.

In addition, the hydrogel capsule for skin moisturizing according to the present invention can effectively block off the odor present in colostrum to prevent the off-flavor in the cosmetic composition, to stably protect and denature the protein mixture from various oxidizing environments of oil and water Has the advantage of not being.

In addition, the hydrogel capsule for moisturizing the skin according to the present invention is stable even in the cosmetic formulation in which various surfactants and oils are present, and has an advantage of implementing high skin absorption efficiency and excellent moisturizing performance when applied to the skin.

In addition, the skin moisturizing hydrogel capsule according to the present invention has an advantage that can be adjusted to the oil-and-moisture balance of the skin when the skin is applied by including the water-soluble protein and fat-soluble components as an active ingredient in one hydrogel capsule.

In addition, the hydrogel capsule for moisturizing the skin according to the present invention has no irritation to the skin by including an active ingredient and a biocompatible material, and is applied to an environmental skin disease such as atopy, thereby realizing excellent performance in moisturizing and improving the skin. It has an excellent effect on skin soothing, moisturizing and healing for infants with atopic dermatitis.

Figure 1 schematically shows a skin moisturizing hydrogel capsule according to an aspect of the present invention.
Figure 2 schematically shows a device for producing a capsule in a continuous phase on the method of manufacturing a hydrogel capsule for skin moisturizing according to an aspect of the present invention.

Unless otherwise defined in the technical and scientific terms used in the present invention, those having ordinary skill in the art to which the present invention belongs have the meanings that are commonly understood, and the gist of the present invention is unnecessary in the following description. Descriptions of well-known functions and configurations that may be blurred are omitted.

As used herein, the singular forms “a”, “an” and “the” are intended to include the plural forms as well, unless the specification indicates otherwise.

The unit of% used without special mention in the present invention means weight% unless otherwise defined.

The inventors of the present invention, while studying the technology for the skin moisturizing composition for improving skin moisturizing and skin troubles, the development and combination of active ingredients effective in moisturizing and improving the skin and the development of an effective delivery system is an important factor It was recognized that. Based on this recognition, researches on delivery systems that can increase the absorption rate of the active ingredient absorbed by the skin and realize the long-term continuous efficacy have been intensified.

As a result, by providing a composite hydrogel capsule containing colostrum-derived protein mixture in the aqueous phase core, comprising a fat-soluble intermediate layer on the aqueous phase core, and comprising a polymer hydrogel capsule layer on the intermediate layer, An active ingredient containing a protein mixture and a fat-soluble component can achieve skin moisturizing and improving effects. Furthermore, by improving the efficiency of being absorbed into the skin, the skin moisturizing ability can be further strengthened. It was found that the present invention was completed.

Hydrogel capsules for skin moisturizing according to the present invention is an aqueous core comprising a protein mixture and a water-soluble polymer derived from colostrum; A fat-soluble intermediate layer located on the water core; It relates to a hydrogel capsule for skin moisturizing comprising; and a polymer hydrogel capsule layer located on the intermediate layer.

In the present invention, when the colostrum-derived protein mixture is absorbed into the skin, the protein mixture has properties that are effective for improving skin by promoting the self-cell activity of the skin as well as moisturizing the skin. However, the protein mixture derived from colostrum tends to be denatured when exposed to the outside, and when it is not reliably protected when applied to the skin, the skin moisturizing performance and skin improvement performance due to the deterioration are deteriorated or are not good for the skin. Can have

Accordingly, the present invention is to provide a hydrogel capsule for moisturizing the skin that can stably capture the protein mixture derived from colostrum to suppress the degeneration of the protein mixture.

Hydrogel capsules for skin moisturizing according to an aspect of the present invention comprises a protein mixture and a water-soluble polymer derived from colostrum in the water core, wrap the water core on the water core to form a fat-soluble intermediate layer, on the fat-soluble intermediate layer It includes the polymer hydrogel capsule layer is formed.

Specifically, as shown in Figure 1, the hydrogel capsule, the aqueous core 10, the fat-soluble intermediate layer 20 and the polymer hydrogel capsule layer 30 is made of a capsule shape having a three-layer structure, the capsule It is characterized in that it contains a water-soluble protein and a fat-soluble component at the same time stably. These components have a high absorption rate to the skin, and can provide a skin's oil and moisture balance, thereby achieving an excellent effect on skin moisturizing and skin improvement.

The aqueous core comprises a protein mixture derived from colostrum. The protein mixture derived from colostrum is obtained from colostrum, for example, may be obtained by separating and purifying from cow colostrum within 3 days immediately after birth, but is not limited thereto.

The protein mixture derived from colostrum is to enhance skin moisturizing performance, to maintain moisturizing performance for a long time, or to play a role of skin improvement, and the present invention does not limit the composition and content range in the protein mixture.

The aqueous core comprises a water soluble polymer with a protein mixture derived from colostrum. The water-soluble polymer may include a water-soluble polysaccharide or a water-soluble synthetic polymer. The water-soluble polysaccharide may be any one or more selected from the group consisting of chitosan, alginic acid, dextran, hydroxypropylmethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, pectin and hyaluronic acid, but is not necessarily limited thereto.

The water-soluble synthetic polymer may be any one or more selected from the group consisting of polyvinyl alcohol and polyethylene oxide. The water-soluble synthetic polymer may have a weight average molecular weight specifically, 10,000 to 1,000,000 g / mol, more specifically 50,000 to 500,000 g / mol, but is not limited thereto. If the above range is satisfied, the water-in-oil type dispersed phase can be kept stable, but this is only one non-limiting example and is not limited to the numerical range.

According to an aspect of the present invention, the aqueous core includes a protein mixture and a water-soluble polymer, the protein mixture may contain 5 to 80% by weight, specifically 20 to 60% by weight relative to the total weight of the aqueous core. If the above range is satisfied, the skin moisturizing and skin improvement as well as a combination of fat-soluble ingredients in capsules to easily adjust the oil and moisture balance of the skin, effective in terms of implementing long-term skin moisturizing performance, but this is only one non-limiting example It is not limited to the above numerical range.

The water-soluble polysaccharide or water-soluble synthetic polymer is not significantly limited in the content in the water core, and can be appropriately adjusted in consideration of the content of the protein mixture. Specifically, the water-soluble polysaccharide or the water-soluble synthetic polymer may include 5 to 80% by weight, specifically 10 to 70% by weight based on the total weight of the aqueous phase core, but is not limited thereto.

The aqueous core may further comprise spirulina extract. The water core has properties that can further enhance moisturizing performance through a combination of spirulina extract with the protein mixture. In particular, long-term continuous moisturizing performance and relieves the heterogeneity in the skin can not only increase the feeling of use, but also helps to soothe and cure environmental skin diseases such as atopic dermatitis, ultimately has an advantageous property for skin improvement.

The spirulina is a blue green cyanobacteria, which has a high protein content and is effective for enhancing skin moisturization and skin improvement desired in the present invention. In addition, the spirulina chelated with the amino acid contained minerals can further increase the skin absorption rate of the effective ingredients in the skin of the capsule, in particular, the compatibility with the protein mixture is excellent in terms of implementing the desired performance of the present invention More effective at

The spirulina is not particularly limited in kind, but may be any one or more selected from spirulina plantn (spirulinaplatensis) and spirulina maxima.

The spirulina extract may be used by pulverizing the collected spirulina extracted by a conventional method. Specifically, a powdery one obtained by mixing spirulina pulverized product with a solvent, followed by heat extraction and filtration and concentration under reduced pressure may be used. The extraction method may be used in combination with any one or two or more selected from hot water extraction, cold extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression, but is not limited thereto. In one embodiment, the mixture obtained by mixing the dried spirulina powder with ethanol in a 1: 5 to 1:20 weight ratio may be obtained from the process of heat extraction at 50 to 70 ° C, preferably 55 to 65 ° C for 6 to 12 hours. have. At this time, the dried spirulina powder and the mixture of ethanol is preferably sonicated to achieve a uniform mixing. In addition, it is more effective to sonicate even after extraction.

The spirulina extract may enhance the compatibility with the protein mixture in the composition by using the aging of the extract obtained by the extraction process at 5 to 20 ° C. for 1 to 7 days, and in view of maintaining long term moisturizing performance Has advantageous properties.

The powdered spirulina extract may be included in an amount of 20 to 200 parts by weight, preferably 50 to 150 parts by weight, based on 100 parts by weight of the protein mixture. When satisfying the above range is effective in terms of maximizing the moisturizing performance as well as long-term moisturizing performance according to the combination with the protein mixture. In addition, it has a skin improvement effect from environmental skin diseases and the like, but this is only one non-limiting example is not limited to the numerical range. The aqueous core may further include a conventional moisturizing component in addition to the protein mixture and the water-soluble polymer, but is not limited thereto.

The fat-soluble intermediate layer is formed to surround the water core, and stably protects the components in the water core, and at the same time has a property that the effective ingredients in the capsule can be quickly absorbed into the skin out of the capsule when the capsule is applied to the skin. .

The fat soluble intermediate layer may include any one or more fat soluble components selected from ceramides, unsaturated fatty acids, tocopheryl oils and vegetable oils.

The unsaturated fatty acid may include any one or more selected from the group consisting of linoleic acid, linoleic acid, docosahexaenoic acid, eicosapentaenoic acid, and oleic acid. It is not limited.

The tocopheryl-based oil may be a compound derived from tocopherol or tocopherol, for example, may be any one or more selected from tocopherol, tocopheryl acetate and tocopheryltinoate, but is not limited thereto.

The vegetable oils include Sacha Inchi oil and Caryodendron Orinocense seed oil, olive oil, camellia oil, castor oil, palm oil, jojoba oil, almond oil, grape seed oil, herbal oil, rose At least one selected from the group consisting of oil, coconut oil, moringa oil, rice bran oil, apricot seed oil, sunflower seed oil, macadamia oil, anato oil, palm oil, safflower oil, meadowfoam seed oil, and abyssinian oil. And the like, but are not limited thereto.

These fat-soluble ingredients have the property of long-term maintenance of the skin efficacy of the capsule according to the present invention in combination with the water-soluble ingredient in the capsule. Specifically, the fat-soluble component includes the components of the aqueous core that are collected therein so that the effective components in the capsule can be evenly dispersed in the skin and absorbed by the skin. At the same time, by absorbing the skin to form a film on the surface of the skin to suppress the evaporation of the active ingredients including moisture to control the oil and moisture balance of the skin, has the effect of implementing the long-term skin moisturizing performance.

Skin moisturizing hydrogel capsule according to an aspect of the present invention is encapsulated into a polymer hydrogel capsule layer. The polymer hydrogel capsule layer is formed on the fat-soluble intermediate layer formed on the water phase core, and may include a water-soluble polysaccharide. In one embodiment, the water-soluble polysaccharide may be a polysaccharide containing a carboxylic acid group. The water-soluble polysaccharide containing a carboxylic acid group has a property that the polymer chain is crosslinked by a reaction with a crosslinking agent, thereby making the capsule layer more robust.

Specific examples of the water-soluble polysaccharide containing a carboxylic acid group may be any one or more selected from the group consisting of pectin and hyaluronic acid. By including the polysaccharide in the capsule layer can not only have a role of a natural moisturizer, but also maintain the polymer hydrogel capsule layer firmly by the reaction with the cross-linking agent, and can stably protect the ingredients effective on the skin in the capsule, It is more effective in keeping the capsule form stable for a long time.

More preferably, the polymer hydrogel capsule layer may further include a water-soluble polysaccharide substituted with a hydrophobic group. In this case, the hydrophobic group may be any one or more hydrophobic functional groups consisting of aliphatic groups and cholesteryl groups, but is not limited thereto. In this case, the hydrophobic group may be substituted by 0.1 to 10 mol% of the repeating units of the water-soluble polysaccharide.

The water-soluble polysaccharide substituted with the hydrophobic group has a hydrophobic group and has an affinity with the fat-soluble intermediate layer inside the capsule layer to have a more stable effect of encapsulating and encapsulating the fat-soluble intermediate layer. In addition, by reducing the contact area with water in the water phase it is possible to reduce the size of the capsule and to increase the uniformity of the capsule.

The hydrophobic group substituted with the hydrophobic group may be any one selected from pectin substituted with a hydrophobic group and hyaluronic acid substituted with a hydrophobic group, and the hydrophobic group may be formed through an ionic bond or a covalent bond.

Specifically, the compound containing a hydrophobic group that can be introduced through the ionic bond is a non-limiting example, lecithin, coco betaine, cetyl trimethylammonium bromide (CTAB), cetyltrimethyl ammonium chloride and dimethyl Dioctadecyl ammonium chloride (Dimethyldioctadecyl ammonium chloride) may be any one or more selected from the group consisting of. These compounds are cationic hydrophobic compounds and have a structure in which hydrophobic groups are grafted with a hydrophobic group stably by ionic bonding with a water-soluble polysaccharide such as pectin or hyaluronic acid having an anionic group.

The compound containing a hydrophobic group which can be introduced through a covalent bond can be used without any limitation as long as it is a substituent that may have hydrophobicity, non-limiting examples, C6 ~ C20 alkyl group, C6 ~ C20 alkenyl group, C6 ~ C20 alkoxy It may be any one or more selected from the group consisting of a nil group and a cholesteryl group. Specifically, the molecule may include an amine group or an isocyanate group. In one embodiment, octadecylamine, palmitoylamine, octyl isocyanate, and the like, but are not limited thereto. The compound has a structure in which the amine group or the isocyanate group forms a covalent bond with the anionic group of pectin or hyaluronic acid through a coupling reaction, so that the hydrophobic group is grafted with the polymer chain stably.

Hydrogel capsules for skin moisturizing according to an aspect of the present invention is not limited in size, but specifically, the average diameter of the capsule may be 10 to 500㎛, more specifically 50 to 400㎛. If the above range is satisfied, it is easy to mix with the cosmetic composition when the capsule is applied to the cosmetic, and effective in moisturizing performance and skin improvement by uniformly applying and absorbing the effective ingredients in the capsule to the skin, but this is only one non-limiting example. It is not limited to the above numerical range.

In addition, the hydrogel capsule for skin moisturizing according to an aspect of the present invention may have a diameter of 1.5 to 15 times the diameter of the water core. That is, the diameter ratio of the water core and the hydrogel capsule may be 1: 1.5 to 1:15, specifically 1: 2 to 1:12. When satisfying the above range has the characteristics that can give the elastic surface and excellent feeling to the skin surface, this is only one non-limiting example is not limited to the numerical range.

Hydrogel capsules for skin moisturizing according to an aspect of the present invention may be applied to the cosmetic composition is applied to the skin. The cosmetic composition is not limited to the formulation, but is not limited to one, skin toner, lotion, cream, essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex It may be any one or more selected from the group consisting of a press powder, a loose powder and an eye shadow.

Another aspect of the present invention relates to a method for preparing a hydrogel capsule for skin moisturizing.

In the method for producing a skin moisturizing hydrogel capsule according to an aspect of the present invention, a water-in-oil type in which an aqueous phase droplet containing a protein mixture derived from colostrum and a water-soluble polymer forms an inner phase, and an oil phase containing a fat-soluble component forms an outer wound. Emulsion forming step;

A dispersed phase injection step of injecting the water-in-oil emulsion through a dispersed phase injection hole;

A continuous phase injection step of injecting a continuous phase containing a water-soluble polysaccharide into an induction pipe communicating with the dispersion phase inlet port at an acute angle to the perpendicular direction at the same time as the dispersed phase injection step;

Discharging the water-in-oil emulsion through the continuous phase through a discharge port located opposite the injection direction of the dispersed phase to form an oil-in-water emulsion; And

And encapsulating the discharged oil-in-water emulsion.

Method for producing a hydrogel capsule according to an aspect of the present invention has the characteristics that can be easily prepared according to the flow rate of the dispersion phase and the diameter of the injection port size of the capsule. In addition, the hydrogel capsule manufactured by the above method stably collects water-soluble and fat-soluble components including a protein mixture therein, and is applied to cosmetics to enhance skin moisturizing efficacy when applied to the skin as well as to be absorbed by the skin. Has the effect of increasing. In particular, it has the effect of effectively blocking off-odor present in the protein mixture derived from colostrum and suppressing denaturation of the protein mixture from various oxidative environments of oil and water phases present in the cosmetic composition.

Specifically, in the water-in-oil emulsion forming step, the aqueous phase containing a mixture of colostrum-derived protein mixture and a water-soluble polymer in distilled water is added to an oil phase containing a fat-soluble component to prepare a dispersed phase in which the aqueous phase droplets are dispersed. An enemy forms an internal phase, and a fat-soluble component surrounds the aqueous droplet to form an external phase to prepare a water-in-oil emulsion. At this time, the protein mixture, the water-soluble polymer and the fat-soluble component derived from colostrum are as described above, and description thereof will be omitted.

In the dispersed phase, the aqueous droplet may be included 5 to 40% by weight, specifically 8 to 30% by weight based on the total weight of the dispersed phase, it can be adjusted to the content range of the protein mixture, etc. in the capsule to be prepared.

The prepared water-in-oil emulsion undergoes a disperse phase injection step injected through a disperse phase inlet, followed by injecting a continuous phase containing a water-soluble polymer from an induction tube connected at an acute angle to a direction perpendicular to the disperse phase injection step. It is subjected to a continuous phase injection step.

Through the continuous phase injection step, the dispersed phase is made into a capsule having a two-layer structure of an aqueous core and a fat-soluble intermediate layer surrounding the aqueous core, which flows from the aqueous phase toward the outlet opposite the injection direction of the dispersed phase inlet. That is, the dispersed phase passing through the continuous phase forms a water-in-oil-in-water emulsion and is discharged through the outlet.

The water-in-oil emulsion may include a water-soluble polysaccharide containing a carboxylic acid group in its outermost phase. It is dropped into an oil bath containing a crosslinking agent through the outlet, and has the property of facilitating the capsule formation in the encapsulation process of the water-in-oil emulsion.

The encapsulation step of the discharged water-in-oil-in-water emulsion is a water-in-oil-in-water emulsion, in the oil phase of the oil bath wraps the outer layer of the two-layer structure of the aqueous core and fat-soluble intermediate layer with an aqueous phase containing a carboxylic acid group in the water phase, It is a process of forming a three-layered capsule consisting of a fat-soluble intermediate layer and a hydrogel capsule layer. In this case, the hydrogel capsule layer may include a water-soluble polysaccharide crosslinked through crosslinking of a water-soluble polysaccharide containing a crosslinking agent and a carboxylic acid group contained in an oil phase.

The method for producing a hydrogel capsule according to an aspect of the present invention may further include a water-soluble polysaccharide substituted with a hydrophobic group in a continuous phase. By further including a water-soluble polysaccharide substituted with a hydrophobic group in the continuous phase, the water-in-oil emulsion in water can be stably maintained because the water-soluble polysaccharide substituted with a hydrophobic group is located at an oil phase-water phase interface. The water-in-oil-in-water emulsion is discharged through an outlet and maintains a stable emulsion structure even when placed in an oil bath, so that the cross-linking agent included in the oil phase forms a stable form while forming a crosslink with a water-soluble polysaccharide containing a carboxylic acid group. By maintaining it has the advantage of finally obtaining a hydrogel capsule without loss of the active ingredient.

The crosslinking agent is not particularly limited as long as it can form a capsule, and specifically, water-soluble calcium salts may be mentioned. The water-soluble calcium salt is advantageous in improving the degree of crosslinking in the encapsulation process, and has properties that can minimize skin irritation. The non-limiting example of the water-soluble calcium salt may be any one or more selected from calcium carbonate, calcium chloride, calcium oxide and calcium hydroxide, but is not limited thereto.

The oil phase containing the crosslinking agent may comprise 0.1 to 3.0 M of calcium salt. Within this range, crosslinking with the carboxylic acid group-containing water-soluble polysaccharide can be efficiently performed smoothly, and a hydrogel capsule layer having a high degree of crosslinking can be formed, so that the effective components in the capsule are stored until delivered to the skin. It has excellent properties. In addition, when satisfying the above range, the hydrogel capsule layer has a characteristic that the degree of crosslinking becomes lower toward the inner center, so that the outermost portion of the hydrogel capsule layer can be easily collapsed by physical pressure upon delivery to the skin. This has the effect of making it easier to release the active ingredient inside the capsule.

The oil phase in which the encapsulation final process is performed may be stirred to facilitate the formation of the hydrogel capsule particles to be finally formed, and the final particles may be obtained by separation through filtering.

Method for producing a skin moisturizing hydrogel capsule according to an aspect of the present invention can be carried out using the microfluidic device disclosed in FIG. That is, the water-in-oil emulsion of the dispersed phase is injected through the dispersed phase inlet 40, and the continuous phase 60 is injected from the induction pipe 41 communicated with the dispersed phase inlet in an acute to right angle to the dispersed phase 50. The final hydrogel capsule is prepared by dropping the water-in-oil-in-water emulsion into the oil phase 70 in the oil bath 80 where the final encapsulation process is carried out, formed into an emulsion and flowing toward the outlet 42 opposite the dispersing phase inlet. Done. The method for producing a molecular hydrogel capsule using the microfluidic device has a property of controlling the size of the capsule produced by controlling the flow rate of the dispersed phase and the diameter of the inlet.

In the microfluidic device, the dispersing phase inlet may adjust the diameter according to the size of the capsule. In addition, the conditions such as the flow rate of the dispersed phase and the continuous phase injected into the microfluidic device, the volume flow rate, the interfacial tension of the dispersed phase and the continuous phase can be appropriately controlled by those skilled in the art, the present invention is not limited thereto.

Hereinafter, the present invention will be described in detail with reference to Examples. However, these are intended to explain the present invention in more detail, and the scope of the present invention is not limited by the following examples. The invention can be better understood by the examples which follow. The following examples are for illustrative purposes of the present invention and are not intended to limit the scope of protection defined by the appended claims.

(Example 1)

1,000 g of colostrum obtained by recovering the liquid from the mammary gland within 24 hours of delivery were subjected to delipidization using a conventional treatment method, and then proteins were selectively separated to obtain a protein mixture.

(Example 2)

100 g of dried spirulina platensis in the form of a dry powder was mixed with ethanol in a 1: 5 weight ratio, and the mixture was extracted for 6 hours at a temperature of 80 ° C. using 100 kHz ultrasonic waves to obtain an extract. The obtained spirulina extract was filtered and concentrated under reduced pressure, and then lyophilized to obtain a powdered spirulina extract.

(Example 3)

A hyaluronic acid solution was prepared by mixing 60 g of distilled water and 10 g of hyaluronic acid (Elizavica Milky Piggy Co., Ltd.), followed by stirring at 100 rpm. Subsequently, the water-based material was mixed with 30 g of the protein mixture according to Example 1 and 400 g of an oil composed of 20% by weight of ceramide, 30% by weight of oleic acid and 50% by weight of sachain oil. (water-in-oil) dispersed phases were prepared. Thereafter, an aqueous solution of hyaluronic acid was prepared in a continuous phase so that 25% by weight of hyaluronic acid was included in distilled water. Next, the dispersed phase and the continuous phase were simultaneously injected using the reactor shown in FIG. 2 (dispersion phase inlet: 300 μm) to form a water-in-oil emulsion after the dispersed phase passed through the continuous water-in-water-in-water emulsion. Dropped into a mineral oil bath containing (oil bath) to prepare the encapsulated particles, and filtered to prepare a hydrogel capsule for final skin moisturizing.

The oil bath was prepared by mixing mineral oil: 4M aqueous solution of calcium chloride at a volume ratio of 4: 1 and irradiating with ultrasonic waves for 1 minute, removing the phase separated water layer and removing water from the mineral oil at 75 ° C. in vacuum.

(Example 4)

In Example 3, the same method as in Example 3 was used except that 20 g of the protein mixture obtained in Example 1 and 20 g of the powdered spirulina extract prepared in Example 2 were mixed with 60 g of a hyaluronic acid solution as a water phase material. Was carried out.

(Example 5)

In Example 3, except that 25% by weight of hyaluronic acid in distilled water and 10% by weight of hydrophobic substituted hyaluronic acid grafted with 5 mole% of octadecylamine group based on the total number of moles of hyaluronic acid were used in a continuous phase. And the same method as in Example 3.

(Comparative Example 1)

60 g of distilled water and 10 g of hyaluronic acid (Elizavica Milky Piggy) were mixed and stirred and mixed at 100 rpm to prepare a hyaluronic acid solution, and then 30 g of the protein mixture according to Example 1 was mixed with the hyaluronic acid solution. The dispersed phase was prepared by dispersing the material in 500 g of an oil phase consisting of 20% by weight ceramide, 30% by weight oleic acid and 50% by weight quaternary oil. Thereafter, an aqueous solution of hyaluronic acid was prepared in a continuous phase so that 25% by weight of hyaluronic acid was included in distilled water. Next, the dispersed phase and the continuous phase were simultaneously injected into the reactor shown in FIG. 2 (dispersion phase inlet: 300 µm), and the dispersed phase passed through the continuous phase contained a protein mixture and hyaluronic acid inside, and an outer layer of the aqueous core. To prepare a capsule layer of a two-layer structure consisting of a fat-soluble intermediate layer consisting of a fat-soluble component.

(Example 6)

20% by weight of the hydrogel capsule prepared in Example 3, 1.5% by weight of glycerin monostearate, 1.5% by weight of cetearyl alcohol, 1.0% by weight of stearic acid, 1.5% by weight of polysorbate 60, 5% by weight of isostearyl isosterate %, Squalane 5% by weight, dimethicone 0.5% by weight, hydroxyethylcellulose 0.1% by weight, glycerin 3% by weight, ceramide 0.3% by weight, triethanolamine 0.3% by weight, mineral oil 25% by weight and the remaining purified water Essence was prepared.

(Example 7)

In Example 6, instead of the hydrogel capsule prepared in Example 3, was carried out in the same manner as in Example 6 except that the hydrogel capsule prepared in Example 4.

(Example 8)

In Example 6, instead of the hydrogel capsule prepared in Example 3, it was carried out in the same manner as in Example 6 except that the hydrogel capsule prepared in Example 5.

Comparative Example 2 In Example 6, instead of the hydrogel capsule prepared in Example 3, was carried out in the same manner as in Example 6 except that glycerin was used.

(Comparative Example 3)

In Example 6, instead of the hydrogel capsule prepared in Example 3, was carried out in the same manner as in Example 6 except that the hydrogel capsule prepared in Comparative Example 1.

(evaluation)

Thirty male and female testers (25 ~ 35 years old) were measured using a Tewameter (TM300, Courage and Khazaka Electronic). The skin barrier was partially removed by repeatedly removing and attaching the 3M tape three times to a predetermined area of the upper arm of each evaluation member. Thereafter, the amount of transdermal water loss was measured in an environment of 23 ° C. and 50% humidity. Select the area of 5 × 5 ㎝ from the constant area of the upper arm of each evaluation source, apply 20 mg of essences according to Examples 3 to 5, Comparative Examples 1 and 2, respectively, and after 6 hours and 12 hours, transdermal moisture again Loss was measured, and the change in transdermal moisture loss was calculated and shown in Table 1 below.

division Example 6 Example 7 Example 8 Comparative Example 2 Comparative Example 3 Transdermal moisture loss (g / hm ² ),
6 hours later 5.2 3.6 4.9 11.9 10.8 Transdermal moisture loss (g / hm ² ),
12 hours later 12.3 9.4 11.7 48.6 44.3

In addition, the skin elasticity performance test was carried out as an experiment on the skin improvement effect a week after using the essence according to Examples 3 to 5, Comparative Examples 1 and 2. The test conditions were 30 male and female testers (25-35 years old) in a constant temperature and humidity room at 22 ° C and a relative humidity of 45%. The measuring device was a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., German). In addition, the feeling of use was evaluated sensually. The evaluation items were described as the average value of the skin texture, the satisfaction with the moisturizing effect and the degree of skin irritation after 10 points of use, respectively, and the results are shown in Table 2 below.

division Example 6 Example 7 Example 8 Comparative Example 2 Comparative Example 3 Skin elasticity 58.8 68.3 66.4 32.8 36.5 Feeling sensory score 8.5 9.4 8.8 5.6 6.7

In addition, an experiment was conducted on the improvement effect on atopic dermatitis. Fifteen patients with atopic dermatitis aged 5 to 20 years old were treated. After washing at a fixed time every day, it was sufficiently applied to the affected area, and the degree of itching and improvement of atopic dermatitis was judged by paperweight and visual observation. The results are shown in Table 3 below. Mean values were described as 10 points out of 10 each for the lack of itching and for the effect of improving atopic dermatitis. As a result, the embodiments according to the present invention obtained very excellent scores on skin elasticity and feeling as well as efficacy on atopic dermatitis. On the other hand, the comparative examples showed a significantly lower score.

division Example 6 Example 7 Example 8 Comparative Example 2 Comparative Example 3 Itching 8.0 9.1 8.2 4.3 4.8 Atopic Dermatitis Improvement 8.4 8.9 8.6 4.2 4.6

Although the preferred embodiment of the present invention has been described above, it is clear that the present invention may use various changes, modifications, and equivalents, and that the above embodiments may be appropriately modified in the same manner. Accordingly, the above description does not define the scope of the invention as defined by the limitations of the following claims.

10: water core, 20: fat-soluble intermediate layer
30: polymer hydrogel capsule layer 40: dispersed phase inlet
41: guide pipe 42: outlet
50: dispersed phase 60: continuous phase
70: oil phase 80: oil bath

Claims (15)

An aqueous core comprising a colostrum derived protein mixture and a water soluble polymer;
A fat-soluble intermediate layer located on the water core; And
A hydrogel capsule for skin moisturizing having a water-in-oil-in-water form including a polymer hydrogel capsule layer positioned on the intermediate layer. The method of claim 1,
The water-soluble polymer of the water core is a hydrogel capsule for skin moisturizing comprising a water-soluble polysaccharide or a water-soluble synthetic polymer. The method of claim 1,
The fat-soluble intermediate layer is a skin moisturizing hydrogel capsule containing one or more selected from ceramides, unsaturated fatty acids, tocopheryl-based oils and vegetable oils. The method of claim 1,
The polymer hydrogel capsule layer is a skin moisturizing hydrogel capsule containing a water-soluble polysaccharide. The method of claim 4, wherein
The water-soluble polysaccharide is a skin moisturizing hydrogel capsule containing a carboxylic acid group-containing polysaccharide. The method of claim 1,
The polymer hydrogel capsule layer is a skin moisturizing hydrogel capsule comprising a cross-linked water-soluble polysaccharide. The method of claim 1,
The polymer hydrogel capsule layer is a hydrogel capsule for skin moisturizing further comprising a water-soluble polysaccharide substituted with a hydrophobic group. The method of claim 1,
The ratio of the diameter of the water core and the hydrogel capsule is 1: 1.5 to 1: 15 hydrogel capsule for skin moisturizing. The method of claim 1,
The water core is a hydrogel capsule for skin moisturizing further comprising spirulina extract. Forming a water-in-oil emulsion in which an aqueous phase droplet containing a protein mixture derived from colostrum and a water-soluble polymer forms an inner phase, and an oil phase containing a fat-soluble component forms an outer phase;
A dispersed phase injection step of injecting the water-in-oil emulsion through a dispersed phase injection hole;
A continuous phase injection step of injecting a continuous phase containing a water-soluble polysaccharide into an induction pipe communicating with the dispersion phase inlet port at an acute angle to the perpendicular direction at the same time as the dispersed phase injection step;
Discharging the water-in-oil emulsion through the continuous phase through a discharge port located opposite the injection direction of the dispersed phase to form an oil-in-water emulsion; And
The encapsulation step of the discharged oil-in-water-in-water emulsion. The method of claim 10,
The water-soluble polysaccharide of the continuous phase injection step of producing a hydrogel capsule containing a carboxylic acid group-containing polysaccharide. The method of claim 10,
The encapsulation step is a method for producing a hydrogel capsule in which the water-in-oil emulsion is crosslinked and encapsulated in an oil bath containing a crosslinking agent. The method of claim 12,
The crosslinking agent is a method for producing a hydrogel capsule is a water-soluble salt containing calcium ions. The method of claim 10,
The continuous phase is a method of producing a hydrogel capsule further comprises a hyaluronic acid substituted with a hydrophobic group or a pectin substituted with a hydrophobic group. Hydrogel capsule prepared by the method of any one of claims 10 to 14.

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