KR102022241B1 - Pharmaceutical composition for preventing and treating type 2 diabetes and non-alcoholic fatty liver - Google Patents

Abstract

The present invention relates to the invention using the extract of the seedling tree, more specifically, it is confirmed that there is a suppression of your biosynthesis and lipid deposition of liver cells of the seedling tree seed extract, and using the same, it is used for diabetes or non-alcoholic fatty liver pharmaceutical The present invention relates to a composition, which can provide a dietary supplement.

Description

Pharmaceutical composition for preventing and treating type 2 diabetes and non-alcoholic fatty liver}

The present invention relates to a method for producing a seedwood seed extract and a composition for preventing and treating diabetic or non-alcoholic fatty liver comprising a seedwood seed extract prepared by the method as an active ingredient, a health functional food.

Diabetes is becoming a major chronic disease year after year. In particular, type 2 diabetes due to obesity is characterized by the destruction of beta cells from insulin resistance (Insulin resistance in type 2 diabetes and obesity: implications for endothelial function). Among these, glucose homeostasis plays a very important role. When glucose homeostasis is broken, hyperglycemia, which is one of the main characteristics of diabetes, occurs. Elevated blood sugars mostly result from abnormal biosynthesis in your liver (Metformin and insulin suppresshepatic gluconeogenesis through phosphorylation of CREB binding protein). AMP-activated protein kinase (AMPK) plays a very important role in energy homeostasis, especially glucose metabolism. AMPK is known to regulate blood glucose by appropriately inhibiting hepatic glycemic synthesis through inhibition of Phosphoenolpyruvate carboxykinase (PEPCK) and Glucose-6-phosphatase (G6Pase) antibodies (Berberine improves glucose metabolism in diabetic rats by inhibition of hepatic gluconeogenesis).

In addition, unsaturated fatty acids are known as a very powerful treatment in the treatment of diabetes (Dietary fats and prevention of type 2 diabetes). However, the mechanism of action of seed tree seed extract on your biosynthesis and lipid synthesis in liver cells has not been elucidated.

Fatty liver disease is also classified into alcoholic fatty liver disease caused by excessive alcohol consumption and nonalcoholic fatty liver disease. Non-alcoholic fatty liver disease is a condition in which triglycerides accumulate in the liver regardless of drinking, and include nonalcoholic fatty liver disease (steatosis) and nonalcoholic steatohepatitis (steatohepatitis).

Nonalcoholic fatty liver disease is highly associated with obesity or diabetes. Since both obese and diabetic patients have been increasing rapidly in recent years, nonalcoholic fatty liver disease is emerging as a disease that is attracting attention in modern society. The incidence of nonalcoholic fatty liver disease rises significantly, from 10 to 15% in people with normal weight and more than 80% in people with overweight. In particular, the prevalence of obesity over the age of 20 increased from 26.3% in 1998 to 31.5% in 2005, and over 30 years increased from 29.1% to 34.8%. Nonalcoholic fatty liver disease is usually recovered by proper intake of nutrients and control of related metabolic diseases. However, if left untreated, hepatitis is followed by fibrosis or hardening of the liver, resulting in poor prognosis. Therefore, in order to prevent the progression of non-alcoholic fatty liver disease to cirrhosis, it is necessary to prevent the accumulation of fat in the liver.However, to prevent the accumulation of fat in the liver, there are other treatment options other than reducing calorie intake or increasing calorie consumption through exercise This situation is not presented.

Korean Registered Notification No. 10-1767604 (2017.08.07) Korean Laid-Open Patent No. 10-2007-0022936 (2007.02.28)

The present inventors have tried to find new natural materials that are effective in preventing and / or treating diabetes. As a result, we find that the seed extract of Shish tree is effective in preventing and / or treating type 2 diabetes, but also in preventing and / or treating nonalcoholic fatty liver. Thus, the present invention has been completed. That is, the present invention is to provide a pharmaceutical composition for the prevention and treatment of diabetes and / or non-alcoholic fatty liver using the Shiraku seed extract.

The present invention to solve the problem relates to a method for producing a seed extract of a tree, a step of washing and drying the seed tree; Two steps of crushing the dried sheath seed; Sheath seed extract may be prepared by performing a process comprising; three steps of obtaining an extract from the shredded tree seed by high temperature milking, low temperature milking or solvent extraction.

In a preferred embodiment of the present invention, the solvent extraction method of step 3 may be obtained by immersing the pulverized seedling tree seed in an organic solvent, and then filtering.

It is another object of the present invention to provide a pharmaceutical composition for preventing and treating diabetes, comprising the extract of Shiraku seed prepared by the method as an active ingredient.

In a preferred embodiment of the present invention, the diabetes may be type 2 diabetes.

In a preferred embodiment of the present invention, by inhibiting the expression of Phosphoenolpyruvate carboxykinase (PEPCK) and G6Pase (Glucose-6-phosphatase) expression, by inhibiting your biosynthesis in liver cells, prevention of type 2 diabetes and It can be cured.

In one preferred embodiment of the present invention, the seed extract of the bark tree may inhibit the expression of caspase-3, which is an apoptosis enzyme.

In a preferred embodiment of the present invention, the seed extract of Shish tree comprises a fatty acid, the fatty acid is 9.70 ~ 10.00% by weight of palmitic acid (palmitoleic acid), 5.00 ~ 5.40 weight of palmitic acid %, Stearic acid 2.50-2.85 wt%, oleic acid 20-23 wt%, linoleic acid 36.00-40.00 wt% and gamma linolenic acid 19-00-23.50 wt% It may include.

It is another object of the present invention to provide a pharmaceutical composition for preventing and treating non-alcoholic fatty liver, which comprises the extract of Shish tree prepared by the method as an active ingredient.

In one preferred embodiment of the present invention, the pharmaceutical composition for preventing and treating non-alcoholic fatty liver is based on the iso-propanol extraction method to reduce the triglyceride accumulation rate of liver cells. As measured by Equation 1 below, when the concentration of the seed extract of the bark tree is 5 μl, when the concentration of TG accumulation is 25% or more, and when the concentration of the seed extract of the bark tree is 10 μl, the rate of TG accumulation is 40% or more. When the seed extract concentration is 20 μl, the TG accumulation reduction rate may be 60% or more.

[Equation 1]

TG accumulation reduction rate (%) = (T ₀ -T ₁ ) / T ₀ Х100 (%)

In Equation 1, T ₀ is the amount of triglyceride accumulation in hepatocytes not treated with Shish seed extract, and T ₁ is the amount of triglyceride accumulation in hepatocytes treated with Shish seed extract.

In addition, the present invention is to provide a dietary supplement for preventing or improving diabetic or non-alcoholic fatty liver, comprising a seed extract of Shish.

Shish seed extract, which is an active ingredient of the present invention, contains a large amount of fatty acids, in particular, palmitic acid, and the shish seed extract suppresses hepatocyte death, inhibits PEPCK (Phosphoenolpyruvate carboxykinase) expression, and G6Pase (Glucose-6-phophatase) expression. It is possible to suppress, it was confirmed that the biosynthesis of the liver (liver) cells, it is possible to provide a composition for preventing and treating diabetes, it is also possible to reduce the triglyceride accumulation of liver (liver) cells Bar, it is possible to provide a composition for preventing and treating non-alcoholic fatty liver.

1 is a result of measuring your biosynthesis-related gene expression inhibitory effect carried out in Experimental Example 2, A is the result of measuring PEPCK expression inhibitory effect, B is the result of measuring G6Pase expression inhibitory effect.
Figure 2 is a result of measuring your biosynthesis inhibition through glucose production rate in liver cells carried out in Experimental Example 3.
Figure 3 is a test result of apoptosis inhibitory effect carried out in Experimental Example 4.
Figure 4 is a test result of the caspase-3 inhibitory effect of apoptosis enzyme carried out in Experimental Example 5.
5 is a result of the inhibition of lipid deposition by measuring the rate of reduction of TG accumulation (Triglyceride accumulation) of liver cells carried out in Experimental Example 6.

Hereinafter, the present invention will be described in more detail.

The present invention is a study on the use of the seed of the tree, the seed extract of the tree contains a large amount of fatty acids such as palmitic acid, the seed extract of the tree is a prophylaxis and treatment for type 2 diabetes and / or non-alcoholic fatty liver It was confirmed that the present invention was completed.

Eu-diadaniellii Hemsl is a deciduous broad-leaved small arborescent tree, belonging to the family of the Hyanghwi family. officinalis Dode ), sewage oil is almost the same shape as shish tree, but is a different species with a small number of small leaves, hairs on the back of the leaves, and round fruits. Seeds of sheath are oval, black, glossy, used as kerosene, mature in October. Shish oil has been used as a head oil, skin disease, or pest control drug in the past, but has not been used as a material for preventing and / or treating diabetes, non-alcoholic fatty liver, and the present invention relates to the invention using such shik wood.

Seed tree seed extract used as an active ingredient of the present invention comprises one step of washing and drying the seed (seed) of the tree; Two steps of crushing the dried sheath seed; It can be prepared by carrying out a process comprising; three steps to obtain an extract from the shredded tree seed.

The pulverization of the second step may be carried out through a general grinding method used in the art, and the method is not particularly limited.

Extraction of the three stages can be carried out through a general method used in the art, a preferred example, it can be obtained by extracting the ash tree seed by high temperature milking, low temperature milking or solvent extraction. In more detail with respect to the extraction method through the solvent extraction method, after immersing the pulverized bark seeds in an organic solvent for 20 to 48 hours, preferably 22 to 30 hours under 15 ℃ ~ 35 ℃, immersion liquid It is possible to obtain the seed extract of the bark tree by filtering. In addition, the extraction process may be repeated 2 to 5 times, preferably 2 to 4 times.

As the extraction solvent, ethanol, methanol, hexane, chloroform, acetone and one or two or more organic solvents selected from these may be used. Preferably, one or two or more organic solvents selected from hexane, chloroform and ethanol may be used. .

The shiitake seed extract prepared in this way contains a large amount of fatty acids, in particular, 9.70 to 10.00 wt% of palmitic acid, 5.00 to 5.40 wt% of palmitoleic acid, and stearic acid (stearic) acid) 2.50-2.85 wt%, 20-23 wt% oleic acid, 36.00-40.00 wt% linoleic acid and 19.00-23.50 wt% gamma linolenic acid, preferably palmitic Acids 9.75 to 9.90 weight percent, palmitoleic acid 5.00 to 5.30 weight percent, stearic acid 2.52 to 2.76 weight percent, oleic acid 20.70 to 22.30 weight percent, linoleic acid 36.50 to 39.60 weight percent and the balance of gamma linolenic acid, more preferably Palmitic acid from 9.75 to 9.85% by weight, palmitoleic acid from 5.00 to 5.20% by weight, stearic acid from 2.55 to 2.72% by weight, oleic acid from 20.80 to 22.10% by weight, linoleic acid 37.20 to 39.50% by weight and the balance of gamma linolenic acid.

The pharmaceutical composition for preventing and treating diabetes mellitus of the present invention comprises a seed extract of Shish tree extracted by the method described above as an active ingredient.

The seed extract of S. chinensis not only inhibits the expression of Phosphoenolpyruvate carboxykinase (PEPCK), which activates the biosynthesis of liver cells, but also inhibits the expression of G6Pase (Glucose-6-phosphatase), thereby inhibiting the expression of gluconeogenesis in liver cells. ) Can be inhibited to prevent and / or treat type 2 diabetes.

In addition, the bark seed extract inhibits caspase-3 (caspase-3), an apoptosis enzyme, and has an effect of inhibiting apoptosis of hepatocytes.

In addition, the present invention can provide a pharmaceutical composition for preventing and / or treating non-alcoholic fatty liver, comprising the extract of Shish-tree seed extracted by the above-described method as an active ingredient, and the non-alcoholic fatty liver prevention and / or treatment effect is It can be confirmed by reducing the amount of TG accumulation (Triglyceride accumulation) of liver cells by the extract treatment. In this case, the liver cells may be preferably HepG2 cells.

More specifically, on the basis of the iso-propanol extraction method (iso-propanol extraction method), by measuring the rate of reduction of triglyceride accumulation of liver cells (Triglyceride accumulation) and calculated by the following equation 1, When the concentration of the seed extract of the bark tree is 5 μl, the TG accumulation reduction rate may be 25% or more, preferably 27% to 35%, more preferably 27.5% to 33%.

In addition, when the concentration of the seed extract of the tree is 10 μl, the TG accumulation reduction rate is 40% or more, preferably 42% to 52%, more preferably 43% to 50%.

In addition, when the concentration of the seed extract of Shish tree is 20 μl, the TG accumulation decrease rate may be 60% or more, preferably 63% to 75%, and more preferably 65% to 73%.

[Equation 1]

TG accumulation reduction rate (%) = (T ₀ -T ₁ ) / T ₀ Х100 (%)

In Equation 1, T ₀ is the amount of triglyceride accumulation in hepatocytes not treated with Shish seed extract, and T ₁ is the amount of triglyceride accumulation in hepatocytes treated with Shish seed extract.

In addition to the pharmaceutical composition using the seed extract of the bark of the present invention, it may also be provided as a nutraceutical for preventing and / or improving diabetes or non-alcoholic fatty liver.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the following Examples are not intended to limit the scope of the present invention, which will be construed as to help the understanding of the present invention.

[ Example ]

Example  One : Tree  Preparation of Seed Extract

Seeds of shii were prepared in October 2016 from the shii plantation in Wolah test forest of the National Forest Research Institute.

Next, the seedlings were washed thoroughly with water and then dried sufficiently.

Next, the dried sheath seed was pulverized using a pulverizer to obtain a pulverized product, and the pulverized product was immersed in a nucleic acid (Hexane) at 25 to 26 ° C. for 24 hours, and then filtered to obtain an extract. Then, the same extraction process was repeated three times to obtain a final seed extract.

Experimental Example  One : Tree  Seed Extract Ingredient Analysis

Shish seed extract of Example 1 was analyzed using gas chromatography, the results are shown in Table 1 below.

fatty acid Fatty acid content (% by weight) Abbreviation Common name C16: 0 Palmitic acid, palmitate 9.80 ± 0.01 C16: 1 Palmitoleic acid 5.04 ± 0.01 C18: 0 Stearic acid 2.61 ± 0.01 C18: 1 Oleic acid 21.40 ± 0.09 C18: 2 Linoleic acid 38.96 ± 0.04 C18: 3 Gamma-linolenic acid 21.95 ± 0.04

Looking at the Table 1, it was confirmed that the seed extract of Shish tree contains various fatty acids, it contains a large amount of palmitic acid.

Experimental Example  2 : Tree  Of seed extract You biosynthesis  Measurement of related gene suppression effect

(1) After receiving HepG2 cells from Korea Cell Line Bank, 10% FBS (fetal bovin serum, Gibco, Life technologies, China), penicillin (Penicillin, 40 unit / ml) and streptomycin (streptomycin, 40 ug / ml) HepG2 cells were inoculated in DMEM (Glucose-Pyruvate free Dulbecco`s modified Eagle's medium) containing the same and cultured in a cell incubator at 37 ° C. with 5% CO ₂ .

(2) The cultured HepG2 cells were treated according to the concentration-dependent concentrations (0 [mu] l, 5 [mu] l, 10 [mu] l, 20 [mu] l) of Shish seed extract prepared in Example 1, followed by quantitative PCR (polymerase chain reaction) method PEPCK (Phosphoenolpyruvate carboxykinase) expression inhibition degree was measured using, and the results are shown in Figure 1A.

(3) In addition, the cultured HepG2 cells were treated with concentrations (0 μl, 5 μl, 10 μl, 20 μl) of the Shish seed extract prepared in Example 1, and then G6Pase ( The degree of inhibition of Glucose-6-phosphatase) expression was measured, and the results are shown in B of FIG. 1.

In Figures A and B of the control group was treated with the solvent used in the seed extract of the tree, PEPCK expression inhibition measurement was measured and calculated the relative amount of PEPCK mRNA expression by quantitative PCR method, G6Pase mRNA expression measurement by the quantitative PCR method Relative G6Pase mRNA expression was measured and calculated.

Looking at A and B of Figure 1, it can be seen that the amount of PEPCK mRNA expression and G6Pase mRNA expression decreases as the concentration of Shish seed extract increases.

Experimental Example  3: through the production rate of glucose in liver cells You biosynthesis  Inhibition measurement experiment

HepG2 cells were incubated for 24 hours at 2 × 10 ⁵ CFU per well, followed by an additional 24 hours in serum free medium.

Next, after wiping twice with PBS, 1 mM sodium pyruvate, 20 mM sodium lactate, 15 mM HEPESdl (4- (2-hydroxyethyl) -1- HepG2 cells are incubated for 4 hours in phenol red-free DMEM (Dulbecco's modified Eagle's medium) containing piperazineethanesulfonic acid.

100 μl of this culture medium was treated with the concentration of the Shiraku seed extract prepared in Example 1 by concentration (0 μl, 5 μl, 10 μl, 20 μl), and then Amplex Red Glucose / Glucose Oxygen. Glucose production in HepG2 cells was measured using a multidose measurement kit (Amplex Red Glucose / Glucose oxidase Assay Kit, Sigma), and the results are shown in FIG. 2 and Table 2 below. In this case, the production rate of the glucose is calculated by percentage based on the control. At this time, the control group was treated with the solvent used in the seed tree seed extract.

Shea Seed Extract Concentration Glucose Production Rate (%) Control 100% 0 μl 263.4% 5 μl 203.7% 10 μl 171.7% 20 μl 107.6%

Looking at the Table 2 and Figure 2, it can be seen that there is a tendency to decrease the intracellular glucose production rate in dependence of the concentration of Shish seed extract, through which the Shish seed extract has an effect of inhibiting your biosynthesis in liver cells Could.

Experimental Example  4: measurement of apoptosis inhibitory effect

After treatment with concentration-dependent concentrations (0 μl, 5 μl, 10 μl, 20 μl) of the Syrup seed extract prepared in Example 1 in 100 μl culture medium, the cells caused by palmitic acid for HepG2 cells The effect of inhibiting death was measured, and the results are shown in Table 3 and FIG. 3. At this time, the control group was treated with the solvent used in the seed extract of the tree, the cell viability was HepG2 using MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay (Sigma) method The cells were counted.

Shea Seed Extract Concentration Liver cell survival rate (%) Control 100% 0 μl 45.1% 5 μl 67.8% 10 μl 73.7% 20 μl 92.3%

Looking at the Table 3 and Figure 3, it can be seen that the survival rate of the hepatocytes tends to increase depending on the concentration of the Shii seed extract, it was confirmed that the Shii seed extract has an effect of inhibiting the death of HepG2 cells. .

Experimental Example  5: Caspase -3 ( caspase -3) inhibition effect measurement

After treating the seed extract prepared in Example 1 in concentration-dependent manner (0 μl, 5 μl, 10 μl, 20 μl) in 100 μl culture medium, the enzyme related to cell death due to palmitic acid Inhibition of Caspase-3 The effect was measured and the results are shown in Table 4 and FIG. 4. At this time, the control group was treated with the solvent used for the seed extract of Cassia, caspase-3 activity was calculated using the Caspase 3 activity assay kit (Abcam).

Shea Seed Extract Concentration Caspase-3 activity (unit) Control 0.077 0 μl 0.383 5 μl 0.138 10 μl 0.235 20 μl 0.217

Looking at the Table 4 and Figure 4, it can be seen that there is a tendency to decrease caspase-3 activity dependent on the concentration of Shish seed extract, through which the Shish seed extract inhibits the activity of apoptosis-related enzymes of HepG2 cells. It was confirmed that there is an effect of suppressing death.

Experimental Example  6: intracellular liver Geological deposition  Inhibitory effect measurement

The amount of triglyceride accumulation in HepG2 cells after 100% of culture medium was treated in a concentration-dependent manner (0µl, 5µl, 10µl, 20µl) of the Syrup seed extract prepared in Example 1 Was measured, and the results are shown in Table 5 and FIG. 5. At this time, the control group was treated with the solvent used in the seed tree seed extract, TG accumulation (Triglyceride accumulation) reduction rate was calculated based on the following equation (1).

 [Equation 1]

TG accumulation reduction rate (%) = (T ₀ -T ₁ ) / T ₀ Х100 (%)

In Equation 1, T ₀ is the amount of triglyceride accumulation in hepatocytes not treated with Shish seed extract, and T ₁ is the amount of triglyceride accumulation in hepatocytes treated with Shish seed extract.

Shea Seed Extract Concentration TG accumulation unit TG accumulation reduction rate (%) Control 0.221 - 0 μl 0.646 0% 5 μl 0.450 30.34% 10 μl 0.351 45.67% 20 μl 0.210 67.49%

Looking at the Table 5 and Figure 5, it can be seen that the concentration of TG accumulation decreases depending on the concentration of Shish seed extract, and that the TG accumulation decrease tends to increase significantly as the Shish seed extract increases. Inhibition of lipid deposition in HepG2 cells, it was confirmed that it can be used as a pharmaceutical composition for preventing and treating non-alcoholic fatty liver.

Through the above examples and experimental examples, the bark seed extract not only has an inhibitory effect on PEPCK expression and G6Pase expression, which are genes related to your biosynthesis, but also has an inhibitory effect on caspase-3, an apoptosis enzyme. It was confirmed that there is a prophylactic and / or therapeutic effect. In addition, it was confirmed that the seed extract of S. chinensis inhibits TG accumulation in liver cells, that is, inhibits lipid deposition, thereby preventing and treating nonalcoholic fatty liver. Such, Shish seed extract can be applied to a variety of pharmaceutical compositions and / or dietary supplements for the prevention and treatment of diabetes or non-alcoholic fatty liver.

Claims (12)

delete delete Eodia daniellii Hemsl seed extract obtained by extraction with a solvent using a solvent containing at least one selected from hexane, chloroform and ethanol as an active ingredient,
The Seed Tree Seed Extract contains a fatty acid, the fatty acid is 9.70 to 10.00% by weight of palmitic acid, 5.00 to 5.40% by weight of palmitoleic acid, and stearic acid 2.50 ~ 2.85 wt%, 20-23 wt% oleic acid, 36.00-40.00 wt% linoleic acid and 19.00-23.50 wt% gamma linolenic acid,
A pharmaceutical composition for preventing and treating type 2 diabetes, characterized by inhibiting your biosynthesis in liver cells through inhibition of Phosphoenolpyruvate carboxykinase (PEPCK) and Glucose-6-phosphatase (G6Pase) expression.
4. The pharmaceutical composition for preventing and treating type 2 diabetes, according to claim 3, which inhibits the expression of caspase-3, which is an apoptosis enzyme.
delete delete It comprises as an active ingredient the seed tree seed extract obtained by extraction with a solvent extraction method using a solvent containing at least one selected from hexane, chloroform and ethanol,
The Seed Tree Seed Extract contains a fatty acid, the fatty acid is 9.70 to 10.00% by weight of palmitic acid, 5.00 to 5.40% by weight of palmitoleic acid, and stearic acid 2.50 ~ 2.85 wt%, 20-23 wt% oleic acid, 36.00-40.00 wt% linoleic acid and 19.00-23.50 wt% gamma linolenic acid,
Based on the iso-propanol extraction method, when the rate of reduction of triglyceride accumulation of liver cells is measured by Equation 1 below,
When the concentration of the seed extract of the bark tree is 5 µl, the decrease in TG accumulation is 25% or more,
When the concentration of the seed extract of the bark tree is 10 μl, the reduction rate of TG accumulation is more than 40%,
Non-alcoholic fatty liver prophylaxis and treatment, characterized in that when the concentration of the seed extract of Shish tree is 20 μl, TG accumulation reduction rate is more than 60%;
[Equation 1]
% Reduction in TG accumulation = (T ₀ -T ₁ ) / T ₀ Х100%
In Equation 1, T ₀ is the amount of triglyceride accumulation in hepatocytes not treated with Shish seed extract, and T ₁ is the amount of triglyceride accumulation in hepatocytes treated with Shish seed extract.
delete delete delete Eodia daniellii Hemsl seed extract obtained by extraction with a solvent using a solvent containing at least one selected from hexane, chloroform and ethanol as an active ingredient,
The Seed Tree Seed Extract contains a fatty acid, the fatty acid is 9.70 to 10.00% by weight of palmitic acid, 5.00 to 5.40% by weight of palmitoleic acid, and stearic acid 2.50 ~ 2.85 wt%, 20-23 wt% oleic acid, 36.00-40.00 wt% linoleic acid and 19.00-23.50 wt% gamma linolenic acid,
A health functional food for preventing and improving type 2 diabetes, characterized by inhibiting your biosynthesis in liver cells by inhibiting Phosphoenolpyruvate carboxykinase (PEPCK) and G6Pase (Glucose-6-phosphatase) expression.
It comprises as an active ingredient the seed tree seed extract obtained by extraction with a solvent extraction method using a solvent containing at least one selected from hexane, chloroform and ethanol,
The Seed Tree Seed Extract contains a fatty acid, the fatty acid is 9.70 to 10.00% by weight of palmitic acid, 5.00 to 5.40% by weight of palmitoleic acid, and stearic acid 2.50 ~ 2.85 wt%, 20-23 wt% oleic acid, 36.00-40.00 wt% linoleic acid and 19.00-23.50 wt% gamma linolenic acid,
Based on the iso-propanol extraction method, when the rate of reduction of triglyceride accumulation of liver cells is measured by Equation 1 below,
When the concentration of the seed extract of the bark tree is 5 µl, the decrease in TG accumulation is 25% or more,
When the concentration of the seed extract of the bark tree is 10 μl, the reduction rate of TG accumulation is more than 40%,
Non-alcoholic fatty liver prevention and improvement health functional food, characterized in that when the concentration of the seed extract of the bark tree is 20 μl, the TG accumulation reduction rate is more than 60%.

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