KR102013274B1 - Skin-whitening cosmetic composition containing centella asiatica extract obtained using focused ultrasonic treatment - Google Patents

Abstract

The present invention relates to a Centella asiatica extract comprising phenolic compounds in a large quantity having effects of inhibiting tyrosinase activity and melanin formation. In particular, the extract is produced by a method comprising the steps of: a) mixing Centella asiatica with water; b) applying focused ultrasound to the mixture obtained in the step a); c) ultrasonically treating the mixture treated with focused ultrasound in the step b); d) applying focused ultrasound to the mixture ultrasonically treated in the step c); and e) ultrasonically treating the mixture treated with focused ultrasound in the step d). Furthermore, the Centella asiatica extract according to the present invention, due to the effects of inhibiting tyrosinase activity and melanin formation, can be beneficially used as a skin-brightening cosmetic material.

Description

SKIN-WHITENING COSMETIC COMPOSITION CONTAINING CENTELLA ASIATICA EXTRACT OBTAINED USING FOCUSED ULTRASONIC TREATMENT}

The present invention relates to Centella asiatica extract containing a large amount of active compound phenolic compound prepared using focused ultrasound. In addition, the present invention relates to a cosmetic composition for skin whitening comprising the extract Centella, since the extract contains a large amount of phenolic compounds having inhibitory effects on tyrosinase activity and melanin production among the components contained in the centella It is excellent in utility as a cosmetic for whitening.

Melanin is a phenolic polymer material widely present in tissues such as skin or eyes of various animals and is a generic term for black to brown pigment.

Melanin is synthesized by the continuous oxidation of tyrosinase enzymes in the melanosomes in the melanocytes of the epidermal basal layer, and has a positive function of protecting the skin by blocking a certain amount of ultraviolet rays, but in modern society aesthetics It is also known as one of the causes of blemishes, freckles, blotch formation and promoting skin aging. Because of these characteristics, the determination of the presence of whitening effect is mainly made by experiments on the ability to inhibit tyrosinase activity and the ability to inhibit melanin production.

Tyrosinase synthesizes melanin precursor by acting as tyrosine hydroxyase which oxidizes tyrosine to make DOPA and DOPA oxidase which oxidizes DOPA to make DOPAquinone, thus acting as an important enzyme in the final synthesis of melanin Done. Specifically, tyrosine is oxidized by tyrosinase to L-3,4-dihydroxyl-Lphenylalanine (L-DOPA), then to DOPAquinone, and then DOPAquinone to DOPAchrome, DOPAchrome to Indole-5,6-quinone or Indole-5, 6-quinone carboxylic acid is finally synthesized melanin.

Phenolic compounds are generally known to have an inhibitory effect on tyrosinase activity and melanin production.Hydroquinone, ascorbic acid and kojic acid are known as inhibitors of tyrosinase activity so far. ), Arbutin, glucosamine and the like. Arbutin and kojic acid are added to the whitening agent in limited amounts due to problems such as skin stability.

Meanwhile, as interest in health and beauty has increased recently, consumers have increased their preference for products containing natural extracts as ingredients rather than synthetic products. As described above, phenolic compounds having inhibitory effects on tyrosinase activity and melanin production are known to be contained in the centella. Therefore, Centella asiatica extract containing phenolic compounds has high utility value as a raw material for cosmetics, foodstuffs and pharmaceuticals.

Accordingly, the need for a method of preparing extracts containing large amounts of phenolic compounds from small amounts of natural products is greatly increasing in the art.

Commonly used methods for extracting natural products include hot water extraction, hot water extraction to elute the active ingredients contained in natural products, solvent extraction using alcohols, and solvents to dissolve and separate the active ingredients in solid or liquid samples. Supercritical extraction using a critical fluid. However, various problems such as low yield, high temperature heat applied to natural products, active ingredients are destroyed or denatured, or organic solvents remaining after extraction do not satisfy the degree of safety suitable for use in human cosmetics. Are present.

In addition, as a method used for extracting natural products, a method of extracting using ultrasonic waves is also disclosed. For example, Korean Unexamined Patent Publication No. 10-2014-0031662 (2014.03.13) discloses a method for preparing red ginseng extract of an organic saponin component, and extracts red ginseng residue after heating red ginseng at high temperature for 4 to 6 times. And adding the organic acid mixed with citric acid and succinic acid and aging, and extracting while giving ultrasonic vibration and microbubbles.

As such, a method of extracting using ultrasonic waves is already known. However, if an organic solvent is used in the extraction process, it is impossible to completely remove the organic solvent from the final product extract, and thus the organic solvent remaining in the extract does not meet the criteria for use as a cosmetic for application to human skin. In addition, when water is used as the extraction solvent without using the organic solvent, the yield is significantly reduced.

In particular, natural extracts vary greatly in the extraction yield and the active ingredient content depending on which plant is the raw material, so even if the extraction method is effective for a particular plant, the same or similar effect may be applied to other plants. In most cases it is not shown.

Publication No. 10-2014-0031662 (2014.03.13)

The present inventors are aware of the problems of the prior art, and to find a method for producing a centrifugal extract containing a large amount of phenolic compound, which is an active ingredient having a whitening effect due to the inhibition of tyrosinase activity and the inhibition of melanin production. The study was repeated through trial and error. As a result, even if water is used as a solvent, it has the effect of inhibiting tyrosinase activity and inhibiting melanin production, thereby demonstrating specific conditions for producing Centella asiatica extract containing phenolic compound having skin whitening effect in a significantly high yield. The present invention has been completed.

According to one aspect of the invention, the invention provides a Centella extract, prepared according to a method comprising the following steps:

a) mixing the centellae with water;

b) irradiating focused ultrasound to the mixture obtained in step a);

c) ultrasonicating the focused ultrasound treated mixture in step b);

d) focusing ultrasonic treatment on the mixture sonicated in step c); And

e) sonicating the focused ultrasonically treated mixture in step d).

The centella is also called tiger grass, clam grass, etc., the scientific name is Centella asiatica . It is a perennial herb in the genus of the herbaceous genus, which has a scale like leaf, 2-5 cm in diameter, inhabiting humid areas such as the southern island of the Korean peninsula. In Korea, the outpost of Centella as medicinal herb is called medicinal herb, and it is known to have the effect of clearing, diarrhea, seedling and detoxification.

According to one aspect of the present invention, Centella asiatica extract using the focused ultrasound of the present invention is characterized in that it has a skin whitening effect, preferably due to the effect of inhibiting tyrosinase activity and inhibiting melanin production, skin whitening effect It is characterized by having.

Each step of the method for producing a centellar extract containing a large amount of phenolic compounds as active ingredients of Centella as a focusing ultrasonic wave according to the present invention will be described in detail.

In the preparation method according to the invention, step a) is a step of mixing the centella and the solvent.

In the manufacturing method according to the present invention, the centellae of step a) may be, but not limited to, a centellae pulverized form.

In the production method according to the invention, the solvent is water. Preferably, organic solvents such as methanol, ethanol, hexane, chloroform, ethyl acetate and the like are excluded from the preparation method according to the present invention.

It is common to use organic solvents as extraction solvents to achieve higher extraction yields of the active ingredients compared to using water. However, the Centella asiatica extract according to the present invention has a purpose for use in cosmetics, so the use of an organic solvent in the extraction process is excluded and water is used as the only extraction solvent. The organic solvent may cause denaturation of the active ingredient in the centella as the extraction proceeds, or may be reacted with the components of the centella to produce adducts, or the organic solvent or a derivative thereof may be added to the extract obtained as a final product. Because it remains, the extract obtained as the final product does not meet the criteria for use as a cosmetic. If the final product obtained by the organic solvent extraction method is used as a cosmetic, an additional step is required to prove that there is no harmful substance to the human body in the final product, or a tablet which completely removes the harmful substance to the human body from the final product. Since further steps are required, it is disadvantageous in terms of the efficiency of the overall process for the production of cosmetics.

The term focused ultrasound used in the present invention is a high-intensity focused ultrasound (HIFU, High Intensity Focused Ultrasound) of 1,000 W / cm ² or more depending on the intensity of the focusing region and low intensity focused ultrasound in the range of 10 mW / cm ² to 50 W / cm ² ( LIFU, Low Intensity Focused Ultrasound). The focused ultrasound used in the method of the present invention is preferably a high intensity focused ultrasound. In particular, the focused ultrasound used in the method of the present invention is more preferably a high intensity focused ultrasound of 1,500 W / cm ³ to 2,000 W / cm ³ . In contrast, the term "ultrasound" refers to sound waves exceeding 20 kHz in the audible range of human hearing.

As shown in the following examples, the inventors performed experiments to measure the extraction yield of the phenolic compound and the content of the phenolic compound in the extract when high-intensity focused ultrasound and general ultrasonic waves were used. As a result, the extraction yield of the phenolic component and the content of the phenolic compound in the extract increased when preparing the centella extract according to the method of the present invention.

In analyzing the rationale for obtaining this result without being bound to a specific theory, focused ultrasound stimulates the cell wall and cell wall space of the plant pool to increase the outflow of active ingredients and increase the fluidity of the solvent. It is believed that phenolic compounds with whitening effect can be more selectively and intensively separated due to the inhibitory effect of tyrosinase activity and melanin pigment production among the components of the Centella as well. .

The focused ultrasound apparatus used in the method according to the present invention includes an ultrasound generating module capable of generating focused ultrasound having a focus at a specific position, the ultrasound generating module including an ultrasonic transducer having an elliptical oscillator.

Preferably, the focused ultrasound apparatus continuously agitates the pool containing solution to prevent the temperature of the medium from rising and the destruction of active ingredient of the centella as the focused ultrasound is focused on a specific position and the focused ultrasound is irradiated. The apparatus can be provided. The stirring device may be, for example, a stirring blade.

Preferably, the focused ultrasound device may be equipped with a cooling device so as to be able to maintain a certain range of temperatures when the method according to the invention is carried out. The cooling device suppresses the temperature rise of the medium by ultrasonic waves or focused ultrasonic waves.

Specific examples of the focused ultrasound device are shown in FIGS. 1 to 7, but are not limited to the devices shown in these drawings.

1 is an example of a focused ultrasound device. As shown in FIG. 1, the ultrasonic processing apparatus 100 includes a processing container 102 in which raw materials to be ultrasonicated are stored, and an ultrasonic wave generating module for irradiating focused ultrasound having a focus on a specific position of the processing container 102 ( 110). For convenience of understanding in FIG. 1, the processing container 102, the ultrasonic wave generating module 110, and the like are shown in cross-sectional view. The ultrasonic wave generating module 110 includes an ultrasonic transducer 116, a cartridge housing 112, an ultrasonic medium 113, and the like. The ultrasonic transducer 116 is provided with an ellipsoidal oscillator 117 to generate focused ultrasound. An ultrasonic transducer 116 is installed inside the cartridge housing 112. The cartridge housing 112 has an opening formed at a lower portion thereof, and a cartridge window 114 for transmitting ultrasonic waves is attached to the opening. The ultrasonic medium 113 is filled inside the cartridge housing 112 and delivers the ultrasonic waves generated by the ultrasonic transducer 116 to the cartridge window 114. In the processing container 102, a coupling part 104 in which the ultrasonic wave generating module 110 is detachably mounted is installed, and the raw material to be ultrasonicated is stored. The ultrasonic wave generating module 110 irradiates the focused ultrasound to extract the active ingredient from the natural product bottle pool stored in the processing container 102. A moving mechanism 118 for moving the ultrasonic transducer 116 is installed inside the ultrasonic wave generating module 110 so that the focused ultrasound can be evenly irradiated to the raw material stored in the processing container 102, or the processing container 102 is provided. A raw material flow device (not shown) that transmits and shakes vibration to the raw materials therein is installed in the processing container 102. The ultrasonic wave generating module 110 is installed such that the cartridge window 114 through which ultrasonic waves pass is in contact with the water surface of the raw material 103 or partially immersed in the raw material. Therefore, the ultrasonic waves generated by the ultrasonic wave generation module 110 are irradiated onto the raw material 103 without passing through the air. The cartridge window 114 is a film of translucent or transparent material that can transmit ultrasonic waves. In this embodiment, the cartridge window 114 may be a Kapton film or another material known to smoothly pass ultrasonic waves. The moving mechanism 118 moves the ultrasonic transducer 116 so that the focused ultrasound generated by the ultrasonic transducer 116 is evenly irradiated to the raw material 103 in the processing container 102. The moving mechanism 118 includes an X axis moving part 118a for moving the ultrasonic transducer 116 in the X axis direction, a Y axis moving part 118b for moving in the Y axis direction, and a Z axis moving for moving in the Z axis direction. It consists of a part 118c. The moving mechanism 118 is configured so that the ultrasonic transducer 116 performs linear movement along the X / Y / Z axis, but the ultrasonic transducer 116 may be configured to move in various forms such as pivot movement, clock movement, spiral movement, and the like. . The moving mechanism 118 is located inside the cartridge housing 112 and is configured to be connected to the ultrasonic transducer 116 to move the ultrasonic transducer 116, but the moving mechanism 118 is the cartridge housing 112. It may be configured to move the cartridge housing 112 to the outside of the. In addition, a raw material flow device (not shown) may be provided to shake the raw material in the processing container 102 so that the focused ultrasound can be evenly irradiated onto the raw material. The raw material flow device may be configured such that the processing vessel 102 performs a clockwise movement, a rotary movement, and the like. In addition, a stirring blade (not shown) for stirring the raw material may be provided in the processing container 102. When the focused ultrasound is irradiated to the raw material 103 in the processing container 102, the temperature of the raw material 103 may rise to generate gas. A gas outlet (not shown) may be formed in the coupling part 104 to discharge the gas. When the ultrasonic wave generating module 110 generates focused ultrasound for a long time, the temperature of the ultrasonic medium 113 is increased, and thus, a cooling mechanism for cooling the ultrasonic medium 113 is required. The medium cooling mechanism is inserted into the cartridge housing 112 into a metal plate 120 in contact with the ultrasonic medium 113 and a heat sink 122 for dissipating heat transferred through the metal plate 120 into the air. It is composed. Peltier elements may be used in place of the heat sink 122. Raw material containers 130, 132, 134 may be further provided. The raw material containers 130 and 132 store raw materials before ultrasonication. The raw material container 134 stores raw materials after ultrasonication. The raw material container 130 is connected to the raw material inlet 137 of the processing container 102 through the transfer pipe 136, and the raw material transfer through the transfer pipe 136 is controlled through the opening / closing valve 144. The raw material container 132 is connected to the raw material inlet 139 of the processing container 102 through the transfer pipe 138, and the raw material transfer through the transfer pipe 138 is controlled through the opening / closing valve 146. The raw material container 134 is connected to the raw material outlet 141 of the processing container 102 through the transfer pipe 140, and the raw material transfer through the transfer pipe 140 is controlled through the opening / closing valve 148. The raw material container 130 and the raw material container 134 are connected through the transfer pipe 142, and the raw material transfer through the transfer pipe 142 is controlled through the opening / closing valve 150. The raw materials stored in the raw material containers 130 and 132 are transferred into the processing container 102 through a pump (not shown) with the open / close valves 144 and 146 open for ultrasonic processing. The raw material ultrasonicated in the processing container 102 is transferred to the raw material container 134 by opening and closing the valve 148. If it is not necessary to perform the ultrasonic treatment repeatedly, once the ultrasonic treatment is not sufficient, the opening and closing valve 150 is opened so that the raw material stored in the raw material container 134 after the ultrasonic treatment to the transfer pipe 142 Transfer to the raw material container 130 through. The raw material transferred to the raw material container 130 is transferred to the processing container 102 through the transfer pipe 136 and ultrasonicated again. That is, the raw material is circulated in the order of the raw material container 134, the transfer pipe 142, the raw material container 130, the processing container 102. In the present embodiment, since the ultrasonic transducer 116 does not directly contact the raw material in the processing container 102, it is possible to suppress generation of harmful components during ultrasonic processing. The focused ultrasound can be uniformly irradiated to the raw material stably stored in the processing container 102 through the moving mechanism 118.

2 is another example of a focused ultrasound device. As illustrated in FIG. 2, the ultrasonic processing apparatus 200 includes a processing container 202 in which raw materials to be ultrasonicated are stored, and an ultrasonic wave generating module for irradiating focused ultrasound having a focus on a specific position of the processing container 202 ( 210. The ultrasonic processing apparatus 200 is different from the ultrasonic processing apparatus 100 shown in FIG. 1 in that the ultrasonic generating module 210 is located below the processing container 202. The ultrasonic wave generating module 210 includes an ultrasonic transducer 216, a cartridge housing 212, an ultrasonic medium 213, and the like. The ultrasonic transducer 216 includes an ellipsoidal oscillator 217 to generate focused ultrasound. An ultrasonic transducer 216 is installed inside the cartridge housing 212, and an opening is formed at an upper portion thereof, and a cartridge window 214 is attached to the opening. The ultrasonic medium 213 fills the interior of the cartridge housing 212 and delivers the ultrasonic waves generated by the ultrasonic transducer 216 to the cartridge window 214. A moving mechanism 218 for moving the ultrasonic transducer 216 is installed in the ultrasonic wave generation module 210 or the raw material in the processing container 202 so that the focused ultrasound can be evenly irradiated to the raw material stored in the processing container 202. A raw material flow device (not shown) that transmits and shakes vibrations is installed in the processing container 202. A coupling part 204 is formed to detachably mount the processing container 202 to the ultrasonic wave generating module 210. The bottom surface of the processing container 202 is provided with a container window 206 through which ultrasonic waves pass. Since the ultrasonic wave generating module 210 is installed to face upward, sag may occur in the cartridge window 214, and a gap may be formed between the cartridge window 214 and the container window 206. When a gap is formed between the windows 206 and 214, ultrasonic waves cannot be delivered in the air and a large loss occurs. To prevent this phenomenon, a medium capable of transmitting ultrasonic waves is filled between the windows 206 and 214. Alternatively, the container window 206 is formed of a film material in which sagging occurs due to the load of the raw material filled in the processing container 202 and is in close contact with the cartridge window 214. That is, the container window 206 is formed by the film material to be in close contact with the cartridge window 214 where the deflection is similar, so that no empty space is formed between the windows 206 and 214. Since the ultrasonic generation module 210 cannot be completely filled with the ultrasonic medium, the cartridge housing 212 may be configured such that the cartridge window 214 is positioned lower than the normal water surface of the ultrasonic medium 213 in the cartridge housing 212. There is a need. To this end, the cartridge housing 212 forms an air pocket 226 such that the upper periphery 225 has a step compared to the cartridge window 214. The moving mechanism 218 moves the ultrasonic transducer 216 so that the focused ultrasound generated by the ultrasonic transducer 216 is evenly irradiated to the raw material in the storage container 202. In this embodiment, the moving mechanism 218 moves in the X-axis direction, the X-axis moving part 218a for moving the ultrasonic transducer 216 in the X-axis direction, the Y-axis moving part 218b for moving in the Y-axis direction, and moving in the Z-axis direction. It consists of the Z-axis moving part 218c. A raw material flow device (not shown) may be provided to shake the raw material in the processing container 202 so that the focused ultrasound can be evenly irradiated onto the raw material. The raw material flow device may be configured such that the processing vessel 202 performs a clockwise movement, a rotary movement, and the like. In addition, a stirring blade (not shown) for stirring the raw material may be provided inside the processing container 202. When focused ultrasound is irradiated to the raw material in the processing container 202, the temperature of the raw material may rise and gas may be generated. A gas discharge port 252 may be formed in the upper portion of the processing container 202 to discharge the gas. In addition, the gas outlet 252 may be provided with a check valve 254 that opens at a pressure of a predetermined size or more. When the ultrasonic wave generating module 210 generates focused ultrasound for a long time, the temperature of the ultrasonic medium 213 is increased, and thus a cooling mechanism for cooling the ultrasonic medium 213 is required. In this embodiment, the medium cooling mechanism is a metal plate 220 inserted into the cartridge housing 112 and in contact with the ultrasonic medium 113, and a heat sink 222 for dissipating heat transferred through the metal plate 220 into the air. It is composed. A Peltier element may be used instead of the heat sink 222. Raw material containers 230, 232, and 234 may be further provided. The raw material containers 230 and 232 store raw materials before ultrasonication. The raw material container 234 stores raw materials after ultrasonication. The raw material container 230 is connected to the raw material inlet 237 of the processing container 202 through the transfer pipe 236, and the raw material transfer through the transfer pipe 236 is controlled through the opening / closing valve 244. The raw material container 232 is connected to the raw material inlet 239 of the processing container 202 through the transfer pipe 238, and the raw material transfer through the transfer pipe 238 is controlled through the open / close valve 246. The raw material container 234 is connected to the raw material outlet 241 of the processing container 202 through the transfer pipe 240, and the raw material transfer through the transfer pipe 240 is controlled through the open / close valve 248. The raw material container 230 and the raw material container 234 are connected through the transfer pipe 242, and the raw material transfer through the transfer pipe 242 is controlled through the opening / closing valve 250. In this embodiment, since the ultrasonic transducer 216 is not in direct contact with the raw material in the processing container 202, it is possible to suppress the generation of harmful components during the ultrasonic processing. In addition, since it is easy to separate the ultrasonic wave generating module 210 from the processing container 202, it is easy to replace the ultrasonic transducer 216. In addition, in the present embodiment, the focused ultrasonic waves may be uniformly irradiated to the raw material stably stored in the processing container 202 through the moving mechanism 218.

3 is another example of a focused ultrasound device. As illustrated in FIG. 3, the ultrasonic processing apparatus 300 includes an ultrasonic wave generating module for irradiating focused ultrasonic waves having a focus on a processing container 202 and a specific position of the processing container 302 in which the raw material to be ultrasonicated is stored ( 310). The ultrasonic wave generating module 310 includes an ultrasonic transducer 316, a cartridge housing 312, an ultrasonic medium 313, and the like. The cartridge housing 312 has a cartridge window 314 formed thereon. The ultrasonic medium 313 fills the interior of the cartridge housing 312 and delivers the ultrasonic waves generated by the ultrasonic transducer 316 to the cartridge window 214. The focus of the ultrasonic wave generating module 310 is that the transducer 316 is aligned with the bottom surface of the processing container 302, that is, near the top surface of the container window 306. An isolation chamber 370 is installed at the bottom of the processing container 302. The isolation chamber 370 has a window through which ultrasonic waves pass. In this embodiment, since the ultrasonic wave generating module 310 is located below the processing container 302, a window is formed at the bottom of the isolation chamber. The focal point of the ultrasonic wave generating module 310 is aligned with the interior of the isolation chamber 370. The isolation chamber 370 includes an inlet 372 through which the raw material flows from the processing container 302, and an outlet 374 through which the raw material flows into the processing container 302. The inlet 372 is provided with an inlet door 376 that opens into the isolation chamber 370, and the outlet 374 is provided with an outlet door 378 that opens to the outside of the isolation chamber 370. In the vicinity of the inflow door 376 or the outflow door 378, raw material conveying fans 380 and 382 may be installed to flow the raw material. The raw materials in the processing container 302 are introduced into the isolation chamber 370 through the inlet 372 by the operations of the raw material conveying fans 380 and 382. The raw material introduced into the isolation chamber 370 is processed by the focused ultrasound while being transported along the isolation chamber, and flows out through the outlet 374. When the focused ultrasound is irradiated to the raw material in the processing container 302, the temperature of the raw material may rise and gas may be generated. A gas discharge port 352 may be formed at an upper portion of the processing container 302 to discharge the gas. In addition, the gas outlet 352 may be provided with a check valve 354 that opens at a pressure of a predetermined size or more. In the present embodiment, the isolation chamber 370 is provided, and the raw material 303 in the processing container 302 is transferred to the isolation chamber 370 and subjected to ultrasonic treatment, whereby the ultrasonic wave can be irradiated more uniformly to the raw material 303.

4 is another example of a focused ultrasound device. As shown in FIG. 4, the ultrasonic processing apparatus 400 includes a processing container 402 in which raw materials to be ultrasonicated are stored, and an ultrasonic wave generating module for irradiating focused ultrasound having a focus on a specific position of the processing container 202 ( 410. The ultrasonic wave generation module 410 includes ultrasonic transducers 416a, 416b, and 416c, an ultrasonic medium 413, and a main housing 411. The ultrasonic medium 413 fills the interior of the main housing 412 and delivers the ultrasonic waves generated by the ultrasonic transducers 416a, 416b, 416c. A moving mechanism 418 for moving the ultrasonic transducers 416a, 416b, and 416c is installed in the ultrasonic wave generating module 410 so that the focused ultrasonic waves can be evenly irradiated to the raw material stored in the processing container 402. An opening is formed in an upper surface of the main housing 412. One end of the inclination adjustment columns 464a and 464b supports the bottom surface of the processing container 402. The inclination adjustment parts 462a and 462b can adjust the inclination of the bottom surface of the processing container 402 with respect to the ground by adjusting the heights of the inclination adjustment columns 464a and 464b. As shown in FIG. 4, when the left side of the bottom surface of the processing container 402 is higher than the right side, the raw material moves from left to right along the bottom surface, so that the raw material is transferred in the processing container 402. There is no need to provide a separate mechanism. The bottom of the processing container 402 is provided with a container window 406 through which ultrasonic waves pass. The processing vessel 402 is installed in the opening of the main housing 412 and the vessel window 406 is immersed in the ultrasonic medium 413. If the bottom surface of the processing vessel 402 is inclined with respect to the ground, the raw material does not accumulate on the bottom surface of the processing vessel 402 and flows along the inclined surface, so the focus of the ultrasonic transducers 416a, 416b, and 416c is located directly above the inclined surface. It is desirable to. The plurality of ultrasonic transducers 416a, 416b, and 416c may be disposed along the inclined surface such that the same raw material is irradiated to the ultrasonic wave several times. Raw material containers 430, 432, and 434 may be further provided. The raw material containers 430 and 432 store raw materials before ultrasonication. The raw material container 434 stores raw materials after ultrasonication. The raw material container 430 is connected to the raw material inlet 437 of the processing container 402 through the transfer pipe 436, and the raw material transfer through the transfer pipe 436 is controlled through the opening / closing valve 444. The raw material container 432 is connected to the raw material inlet 439 of the processing container 402 through the transfer pipe 438, and the raw material transfer through the transfer pipe 438 is controlled through the opening / closing valve 446. The raw material container 434 is connected to the raw material outlet 441 of the processing container 402 through the transfer pipe 440, and the raw material transfer through the transfer pipe 440 is controlled through the opening / closing valve 448. The raw material container 430 and the raw material container 434 are connected through the transfer pipe 442, and the raw material transfer through the transfer pipe 442 is controlled through the opening / closing valve 450. With respect to the delivery tube 436, the delivery tube 438 is connected to the same side of the processing vessel 402 and the delivery tube 440 is connected to the opposite side. That is, the raw material inlets 470 and 472 are formed on the same side of the processing container 402, and the raw material outlet 474 is formed on the opposite side. The guide member 474 is further provided so that the raw material injected through the raw material injection holes 470 and 472 flows down the wall instead of directly falling to the bottom surface of the processing container 402.

FIG. 5 is another example of the focused ultrasound apparatus, and FIG. 5A is a configuration diagram of the ultrasonic processing apparatus 500, and FIG. 5B is a configuration diagram of the ultrasonic generation module 504 used in the ultrasonic processing apparatus 500. As illustrated in FIG. 5A, the ultrasonic processing apparatus 500 includes a processing container 502 in which raw materials to be ultrasonicated are stored, and an ultrasonic wave generating module for irradiating focused ultrasound having a focus on a specific position of the processing container 502 ( 504 and an ultrasonic reflection member 510. The ultrasonic reflective member 510 includes an ultrasonic transducer 516, a cartridge housing 512, an ultrasonic medium 513, and the like. The cartridge housing 512 has a cartridge window 514 formed at the bottom thereof, and an ultrasonic transducer 516 is installed therein. The ultrasonic medium 513 fills the interior of the cartridge housing 512 and delivers the ultrasonic waves generated by the ultrasonic transducer 516 to the cartridge window 514. A moving mechanism 506 is provided to move the ultrasonic wave generation module 504 so that the focused ultrasound can be evenly irradiated to the raw material stored in the processing container 502. In the ultrasonic processing apparatus 500, at least a part of the ultrasonic wave generating module 504 is moved by the moving mechanism 506 in a state of being immersed in the raw material inside the processing container 502. The ultrasonic reflection member 510 is connected to and installed in the ultrasonic generation module 504. The ultrasonic reflection member 510 moves together with the ultrasonic wave generation module 504 and is located in the ultrasonic irradiation direction of the ultrasonic wave generation module 504 to reflect the ultrasonic waves past the focus of the focused ultrasonic wave in the opposite direction. The ultrasonic reflection member 510 may be formed in a “c” shape. The ultrasonic reflection member 510 is installed so that one surface thereof faces in the irradiation direction of the ultrasonic waves to reflect the ultrasonic waves. The ultrasonic reflection member 510 allows the ultrasonic waves to be more uniformly irradiated into the raw material. The stirring unit 508 is installed outside the ultrasonic wave generating module 504. The stirring unit 508 mixes the raw materials when the ultrasonic wave generating module 504 is moved by the moving mechanism 506 so that ultrasonic waves are uniformly irradiated onto the raw materials. As shown in FIG. 5B, the ultrasonic wave generating module 504 is connected by the moving mechanism 506 through the moving shaft 518, and the stirring unit 508 is installed to protrude outside the ultrasonic wave generating module 504. do. The stirring parts 508a, 508b, 508c, and 508d are radially symmetrically installed with respect to the ultrasonic wave generating module 504, and may have different directions of a spoon-shaped end portion. In this embodiment, since the ultrasonic generating module 504 is moved by the moving mechanism 506 while a part of the ultrasonic generating module 504 is locked to the raw material inside the processing container 502, the ultrasonic generating module 504 is the processing container. A function of stirring the raw material in 502 is performed. Therefore, the ultrasonic processing apparatus 500 can uniformly irradiate ultrasonic waves to a raw material with a simple structure.

6 is another example of a focused ultrasound device. As illustrated in FIG. 6, the ultrasonic processing apparatus 600 includes a processing container 602 for storing raw materials to be ultrasonicated, and an ultrasonic wave generating module 604 for generating focused ultrasound. The ultrasonic processing apparatus 600 is provided with the raw material raising / lowering mechanism 606 which moves the processing container 602 up / down. In addition, the ultrasonic processing apparatus 600 further includes a support 608 for supporting the processing container 602 and the ultrasonic wave generating module 604. The support 608 includes a base portion 610 in which the processing container 602 is positioned at an upper portion thereof, and a support portion 612 standing on one side of the base portion 610 to support the ultrasonic wave generating module 604. A moving wheel 614 is installed below the base part 610. The support part 612 is provided with an ultrasonic moving mechanism 616 for moving the ultrasonic wave generating module 604. The ultrasonic wave generating module 604 is installed to the moving mechanism 616 through the support 626. A stirring member 618 is further provided for stirring the raw materials in the processing container 602. The stirring member 618 is rotated by a motor (not shown), has a propeller shape, and a plurality of stirring members 618 may be installed at regular intervals. The stirring member 618 is connected with the ultrasonic wave generating module 604 by the support 624, and moves together when the ultrasonic wave generating module 604 is moved by the moving mechanism 616 to allow the raw materials to be mixed better. The ultrasonic processing apparatus 600 further includes a temperature sensor 620 for sensing the temperature of the raw material in the processing container 602 and a cooling unit 622 for cooling the temperature of the raw material. One example of the cooling unit 622 is installed in the processing vessel 602 to directly cool raw materials stored in the processing vessel 602. The cooling unit 622 may be implemented with, for example, a Peltier element. Another example of the cooling unit 622 is as illustrated in FIG. 1 or 2, while cooling the raw material while transferring the raw material in the processing container 602 to an external storage container through a transfer pipe, and back to the processing container 602. It may consist of injecting.

FIG. 7 is a conceptual diagram of the ultrasonic wave generating module 604 applied to the ultrasonic processing apparatus 600 of FIG. 6. The ultrasonic generating module 604 includes a plurality of ultrasonic transducers 706 in the cartridge housing 702 filled with the ultrasonic medium 704. The plurality of ultrasonic transducers 706 are located at different heights or different radii of curvature of the ultrasonic oscillator have different focal lengths from the cartridge window 708. In addition, the plurality of ultrasonic transducers 706 may generate ultrasonic waves at different intensities. The ultrasonic transducer 706 is located within the cartridge housing 702 and may be installed to irradiate the ultrasonic wave to the upper side or the lower side of the cartridge housing 702. As in the present embodiment, when the ultrasonic transducer 706 irradiates the lower side of the cartridge housing 702, the cartridge window 708 is installed on the lower surface of the cartridge housing 702. When the ultrasonic transducer 706 irradiates ultrasonic waves to the upper side of the cartridge housing 702, the cartridge window is installed on the upper surface of the cartridge housing 702. The ultrasonic wave generation module 604 supplies power to the plurality of ultrasonic transducers 706 through the power line 708 and a control signal from a controller (not shown) through the control line 710. Further comprising a medium circulation line 714 capable of circulating the ultrasonic medium 703 in the cartridge housing 702, the medium circulation line 714 is provided with a temperature sensor 712 for measuring the temperature of the ultrasonic medium. . The medium circulation line 714 may be configured by tying the power line 708 and the control line 710 into one cable form. The medium circulation line 714 receives temperature information from the temperature sensor 712, and when the medium inside the cartridge housing 702 is heated above a predetermined temperature by ultrasonic waves, the medium is circulated to the outside to appropriately adjust the temperature of the medium. Keep at temperature. The ultrasonic generation module 604 is located in the cartridge housing 702 and further includes a check valve 716. The check valve 716 discharges gas generated inside the cartridge housing 702. The ultrasonic medium can be heated by ultrasonic waves to produce a gas. The gas will be present as bubbles inside the cartridge housing 702, thus preventing the transmission of ultrasonic waves.

In the method according to the invention, in the step a) the centella and water are mixed in a weight ratio of 1: 1 to 1:15, preferably, the weight ratio is 1:10. At this weight ratio, the centellae is best mixed without being immersed in a solvent, and focused ultrasound is irradiated to the cell wall of the centellae so that the active ingredient is best eluted.

In the method according to the invention, the ultrasonic waves in steps c) and e) are preferably ultrasonic waves in the range of 10 mW / cm ² to 500 W / cm ² . Ultrasound is believed to play a role in creating an environment that stimulates the cell walls of the pools so that active ingredients can be more easily eluted when focused ultrasound is irradiated.

In the method according to the present invention, the steps b) to e) are preferably performed at 27 ° C. to 70 ° C., more preferably at a temperature of 35 ° C. to 45 ° C. The application of heat at a temperature above 70 ° C. increases the extraction yield or shortens the extraction process time. However, raw materials such as plants or natural products, in particular the active ingredients contained therein, are generally modified by heat. There is an important problem. Thus, performing steps c) to f) at a low temperature of 27 ° C. to 70 ° C., in terms of yield and purity, as compared to other processes requiring heat above 70 ° C., and the integrity of the active ingredient in the final product Very advantageous in terms of

In the method according to the present invention, a separate device such as a cooling device may be necessary to perform the steps b) to e) at a low temperature of 27 ° C to 70 ° C. Irradiation with ultrasound or focused ultrasound often raises the temperature of the medium, which must be kept within a certain range.

In the method according to the invention, the steps b) to e) may be performed for 5 to 30 minutes respectively. The most preferred time in terms of yield and purity of the active ingredients of the final product and the efficiency and productivity of the overall extraction process is about 15 minutes.

According to the present invention, the centellar extract containing the active ingredient phenolic compound in high yield prepared by using focused ultrasound extracts of the phenolic compound compared to the centella extract prepared by other hot water extraction, organic solvent extraction, ultrasonic extraction, etc. The yield and the content of phenolic compounds in the extract are greatly increased.

In addition, the Centella extract prepared using focused ultrasound according to the present invention, since the phenolic compounds directly related to the inhibition of tyrosinase activity and the inhibition of melanogenesis among the active ingredients contained in the Centella are selectively and intensively extracted. It can have an excellent effect on skin whitening. Therefore, Centella asiatica extract using the focused ultrasound according to the present invention is excellent in utility as a cosmetic for skin whitening.

1 to 7 show examples of focused ultrasound devices that can be used in the method according to the invention.
8 shows the results of content analysis of phenolic compounds of Centella asiatica extract prepared in Example 1 and Comparative Example 1. Focused ultrasonic extract refers to an extract extracted using focused ultrasonic waves, ultrasonic extract refers to an extract extracted using ultrasonic extraction, and hot water extract refers to an extract extracted using hot water extraction.
Figure 9 shows the results of the content analysis of the phenolic compound of the soybean extract prepared in Comparative Example 2. Focused ultrasonic extract refers to an extract extracted using focused ultrasonic waves, and hot water extract refers to an extract extracted using a hydrothermal extraction method.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples. It is also to be understood that the terms not specifically defined herein have a meaning commonly used in the technical field to which the present invention belongs.

All experimental results in the present specification were represented by the average value repeated three or more times, and the statistical significance test between the control group and the experimental group was performed at the p <0.05 level using the One-way ANOVA test.

Example 1

The dried centella was washed with purified water and then ground and added to 10-fold weight of distilled water.

Then, the focused ultrasound generated 1500 ~ 2000 W / cm ² through the ellipsoidal oscillator provided in the ultrasonic transducer of Figure 1 was irradiated with the centrifugal solution at 40 ℃ for 15 minutes. At this time, the centellar solution was continuously stirred so that the focused ultrasound was uniformly irradiated on the entire centellar solution.

Thereafter, the focused sonicated centellar solution was sonicated at 40 ° C. for 10 minutes.

Subsequently, while stirring the sonicated centellar solution, focused ultrasonic waves of 1500-2000 W / cm ² generated through an elliptical oscillator were irradiated at 40 ° C. for 15 minutes.

Thereafter, the focused sonicated centellar solution was sonicated at 40 ° C. for 5 minutes.

The resulting Centellar solution was filtered using qualitative filter paper No. 2 (manufacturer: Whatman), and then concentrated under reduced pressure using a rotary evaporator (EYELA, Japan) to completely remove distilled water, and then freeze-dried to -20. Stored at ℃ was used.

Comparative Example 1

In order to compare the extraction yield of phenolic compounds according to the extraction method, the content of phenolic compounds in the extract, the inhibition rate of tyrosinase activity and the melanin production rate, the centella extracts were prepared by the ultrasonic extraction method and the hot water extraction method.

1-1. Preparation of Extracts Using Ultrasonic Extraction

The dried centella was washed with purified water and then ground and added to 10-fold weight of distilled water.

Thereafter, the obtained centrifugal solution was sonicated at 40 ° C. for 45 minutes using an ultrasonic extractor (Ilshin Lab, Daejeon, Korea). In Comparative Example 1-1, ultrasonic waves of 500 W / cm ² were used.

The resulting Centellar solution was filtered using qualitative filter paper No. 2 (manufacturer: Whatman), and then concentrated under reduced pressure using a rotary evaporator (EYELA, Japan) to completely remove distilled water, and then freeze-dried to -20. Stored at ℃ was used.

1-2. Preparation of Extract Using Hot Water Extraction Method

The dried centella was washed with purified water and then ground and added to 10-fold weight of distilled water.

Then, the centellar solution was extracted with hot water at 40 ° C. for 45 minutes.

The resulting Centellar solution was filtered using qualitative filter paper No. 2 (manufacturer: Whatman), and then concentrated under reduced pressure using a rotary evaporator (EYELA, Japan) to completely remove distilled water, and then freeze-dried to -20. Stored at ℃ was used.

Comparative Example 2

To compare tyrosinase activity inhibition rate and melanin production rate according to raw materials, soybean extracts were prepared by using focused ultrasound extraction and hot water extraction.

2-1. Preparation of Extract Using Focused Ultrasound

Soybean extract was prepared by treating dried soybeans in the same manner as in Example 1.

2-2. Preparation of Extract Using Hot Water Extraction Method

Soybean extract was prepared by treating dried soybean in the same manner as in Comparative Example 1-2.

Experimental Example 1. Determination of phenolic compound extraction yield and content

The phenolic compound extraction yields and contents of the active ingredient were confirmed for each of the Centella extract extracts obtained in Example 1 and Comparative Example 1, which are shown in Table 1 and FIG. 8.

sample menstruum Ultrasonic Power (W) Extraction temperature (℃) Active ingredient yield (%) Example 1 water 1800 40 11.89 ± 0.58% Comparative Example 1-1 water 500 40 9.24 ± 0.88% Comparative Example 1-2 water - 40 5.23 ± 0.72%

As can be seen in Table 1, the centella extract prepared in Comparative Example 1-2 according to the hot water extraction method was calculated to contain 5.23 ± 0.72% by weight of the phenolic compound as an active ingredient.

According to the ultrasonic extraction method, the Centella extract prepared in Comparative Example 1-1 was about 1.76 times higher in extraction yield of the phenolic compound as an active ingredient than the Centella extract prepared in Comparative Example 1-2.

According to the method of the present invention using focused ultrasound, Centella extract prepared in Example 1 was confirmed that the extraction yield of the active ingredient is about 2.27 times higher than in Comparative Example 1-2, compared to Comparative Example 1-1 It was confirmed that the extraction yield of the phenolic compound as an active ingredient significantly increased.

Accordingly, the Centella asiatica extract prepared according to the method of the present invention using focused ultrasound is proved to have a high extraction yield of the phenolic compound as an active ingredient as compared to the Centella asiatica extract prepared using another extraction method.

In addition, the Folin Ciocalteau method was performed according to the following procedure to confirm the content of the phenolic compound in the extract.

50 μl of test sample was mixed with 100 μl 50% Folin Ciocalteau reagent and left at room temperature for 2 minutes. Then, after mixing with 2 ml 2% sodium carbonate and allowed to stand at room temperature for 30 minutes, the content of phenolic compound was measured at absorbance 720 nm. Gallic acid was used as a standard of the calibration curve, and the content of the phenolic compound in the extract was quantified in comparison with Gallic acid equivalents (GAE), and the results are shown in FIG. 8.

As can be seen in Figure 8, the centella extract prepared in Example 1 according to the production method of the present invention using a focused ultrasound has a content of the phenolic compound compared to the centella extract prepared in Comparative Example 1-2 according to the hot water extraction method It was confirmed that about 2.5 times more, and it was confirmed that the content of phenolic compounds was significantly increased in comparison with the centella extract prepared in Comparative Example 1-1 according to the ultrasonic extraction method.

Accordingly, the Centella asiatica extract obtained according to the preparation method of the present invention using focused ultrasound has proved that the content of the phenolic compound in the extract is higher than that of the extract prepared by other extraction methods.

Experimental Example 2. Comparison of Tyrosinase Activity Inhibition Rate

2-1. Inhibition of Tyrosinase Activity in vitro

Inhibition of tyrosinase activity was measured using the dopachrome method according to the following procedure.

0.2 ml of mushroom tyrosinase (110 U / ml) was added to 0.5 ml of 0.175 M sodium phosphate buffer (pH 6.8), 0.2 ml of 10 mM L-DOPA and 0.1 ml of sample solution. Dopachrome was measured at a wavelength of 475 nm.

The inhibition rate of tyrosinase activity was calculated by the following formula, and the inhibition rate of 100% means that the activity of the enzyme was completely inhibited by sample addition.

Tyrosinase activity inhibition rate (%) = [(C-D)-(A-B) / (C-D)] × 100

A: absorbance of the sample addition solution after the reaction

B: absorbance of the sample addition solution before the reaction

C: Inclusion degree of sample-free solution before reaction

D: absorbance of sample-free solution after reaction

2-2. Comparison of Tyrosinase Activity Inhibition Rate by Different Extraction Methods

The tyrosinase activity inhibition rate was confirmed for each of the Centella extract samples obtained in Example 1 and Comparative Example 1, which is shown in Table 2.

Inhibition rate of tyrosinase activity (%) Example 1 61.52 ± 0.72% Comparative Example 1-1 51.11 ± 1.02% Comparative Example 1-2 53.22 ± 0.78%

As can be seen in Table 2, the centella extract prepared in Example 1 according to the method of the present invention using focused ultrasound compared to the centella extract prepared in Comparative Example 1-2 according to the hot water extraction method inhibition rate of tyrosinase activity This is an increase of about 8.3%.

It is particularly noteworthy that the Centella extract prepared in Comparative Example 1-1 according to the ultrasonic extraction method has about 1.9 times more content of the phenolic compound in the extract compared to the Centella extract prepared in Comparative Example 1-2 according to the hot water extraction method (Fig. Despite this, tyrosinase activity inhibition was found to be reduced by about 2.11%. This means that the content of total phenolic compounds in the extract has increased but not the selective and intensive extraction of phenolic compounds directly related to tyrosinase inhibition. Accordingly, when using focused ultrasound for the preparation of Centella asiatica extract, it is demonstrated that phenolic compounds directly related to the inhibition of tyrosinase activity can be selectively and intensively extracted, which is according to the preparation method of the present invention Proves to be an excellent whitening effect due to inhibition of tyrosinase activity.

2-3. Comparison of Tyrosinase Activity Inhibition Rate with Different Raw Materials

The tyrosinase activity inhibition rate was confirmed for each soybean extract sample obtained in Comparative Example 2, which is shown in Table 3. In addition, the content of the phenolic compound in the soybean extract is shown in FIG.

Inhibition rate of tyrosinase activity (%) Example 1 61.52 ± 0.72% Comparative Example 1-2 53.22 ± 0.78% Comparative Example 2-1 56.26 ± 0.98% Comparative Example 2-2 53.28 ± 0.87%

As can be seen in Table 3 and Figure 9, the soybean extract prepared in Comparative Example 2-1 according to the production method of the present invention using the focused ultrasound compared to the soybean extract prepared in Comparative Example 2-2 according to the hot water extraction method Phenolic compound content in the extract showed a 1.9-fold increase, but the tyrosinase activity inhibition rate increased only about 2.98%. In the case of Example 1 using the centella as a raw material, the content of the phenolic compound in the extract increased 2.5 times compared to Comparative Example 1-2, but the tyrosinase activity inhibition rate is shown to be significantly different from that of about 8.3% more.

This means that when soybean is used as a raw material, the content of the phenolic compound in the total extract is increased, but the phenolic compound directly related to the inhibition of tyrosinase activity is not selectively and intensively extracted. Accordingly, it is demonstrated that the use of centella as a raw material and focused ultrasound for the preparation of centella extract can selectively and intensively extract phenolic compounds directly related to the inhibition of tyrosinase activity. The Centella asiatica extract prepared according to the method is able to exert an excellent whitening effect due to the inhibitory effect of tyrosinase activity.

Experimental Example 3. Comparison of melanin biosynthesis inhibition rate

3-1. Melanin biosynthesis inhibition rate measurement

Clone M-3 (mouse, Cloudman S91, skin, melanoma) cells, a mouse-derived malignant melanoma cell line, used in this experiment were incubated at 37 ° C with 5% CO ₂ in RPMI 1640 medium supplemented with 10% FBS and antibiotics. Incubated at. Cells were passaged after growth to about 80% of the culture dish every 2-3 days.

Cultured M-3 cells were treated with extracts after dispensing 1 × 10 5 cells / well of cells per well into 24-well plates and incubated for 48 hours in a 5% CO ₂ incubator at 37 ° C. Thereafter, each well was washed with PBS, treated with trypsin-EDTA, centrifuged at 5,000 rpm for 10 minutes, and then the supernatant was removed, and the pellet was dried at 60 ° C., followed by 400 μl of 0.2N NaOH solution. 10% DMSO) was added and dissolved at 60 ° C. for 1 hour.

Then, the absorbance was measured at 405 nm with a Microplatereader (Molecular Devices, USA) to calculate the amount of melanin produced from the cells, and then the relative melanin production rate was calculated by the following equation.

Relative melanin production rate (%) = (A / B) × 100

A: amount of melanin after extract addition (ug / mL)

B: amount of melanin without extract (ug / mL)

3-2. Comparison of Melanin Production Rate by Extraction Method

The melanin production rate of each of the Centella extract extracts obtained in Example 1 and Comparative Example 1 was confirmed and is shown in Table 4.

Melanin production rate (%) Example 1 74.51 ± 0.41% Comparative Example 1-1 79.61 ± 0.11% Comparative Example 1-2 82.45 ± 0.43%

As can be seen in Table 4, the centella extract prepared in Comparative Example 1-2 according to the hot water extraction method was calculated to produce a melanin of about 82.45 ± 0.43%.

According to the ultrasonic extraction method, the centella extract prepared in Comparative Example 1-1 was found to have reduced melanin production rate by about 2.84% compared to the centella extract prepared in Comparative Example 1-2.

According to the preparation method of the present invention using focused ultrasound, the Centella extract prepared in Example 1 was confirmed that the melanin production rate was reduced by about 7.94% compared to Comparative Example 1-2, compared to Comparative Example 1-1 It was confirmed that melanin production rate was reduced by about 5.1%.

Accordingly, it is proved that the use of focused ultrasound for the preparation of centella extract effectively inhibits melanin production in comparison with the extract extracted by the ultrasonic extraction method and the hot water extraction method, which is a centrifugal extract prepared using the focused ultrasound of the present invention. Proves to have an excellent whitening effect due to inhibition of melanin production.

3-3. Comparison of Melanin Production Rate by Raw Material

The melanin production rate was confirmed for each of the soybean extract samples obtained in Comparative Example 2, which are shown in Table 5. In addition, the content of the phenolic compound in the soybean extract is shown in FIG.

Melanin production rate (%) Example 1 74.51 ± 0.41% Comparative Example 1-2 82.45 ± 0.43% Comparative Example 2-1 89.28 ± 0.21% Comparative Example 2-2 91.25 ± 0.56%

As can be seen in Table 5 and Figure 9 soybean extract prepared in Comparative Example 2-1 according to the production method of the present invention using the focused ultrasound extract in comparison with the soybean extract prepared in Comparative Example 2-2 according to the hot water extraction method The content of the phenolic compound was 1.9 times increased, but melanin production was reduced only by 1.97%. In the case of Example 1 using the centella as a raw material, the content of the phenolic compound in the extract increased 2.5 times compared to Comparative Example 1-2, but the melanin production rate is about 7.94% further shows a big difference. This means that when soybean is used as a raw material, the content of the total phenolic compound in the extract is increased, but the selective and intensive extraction of the related phenolic compounds directly related to the inhibition of melanogenesis is not possible. Accordingly, it is demonstrated that when using the centella as a raw material and using focused ultrasound for the preparation of centella extract, it is possible to selectively and intensively extract phenolic compounds directly related to the inhibition of melanogenesis, which uses the focused ultrasound of the present invention. It is proved that Centella extract prepared by the present invention can exert an excellent whitening effect due to the melanin production inhibitory effect.

Claims (10)

a) mixing the centellae with water;
b) irradiating high intensity focused ultrasound to the mixture obtained in step a);
c) treating the high-strength focused ultrasound treated mixture with ultrasonic waves in step b);
d) irradiating high intensity focused ultrasound to the mixture sonicated in step c); And
e) a soybean extract prepared according to the method comprising the step of sonicating the high intensity focused ultrasound treated mixture in step d). The method of claim 1,
Centella extract of the a) step is characterized in that the pulverized. The method of claim 1,
Centella extract, characterized in that for stirring the mixture while irradiating high-intensity focused ultrasound in step b) and d). The method of claim 1,
In the step a) Centella and water extract Centella extract, characterized in that mixing in a weight ratio of 1:10. The method of claim 1,
Wherein b) to e) Centella extract, characterized in that carried out at a temperature of 40 ℃. The method of claim 1,
Wherein b) to e) step Centella extract, characterized in that performed for 5 to 30 minutes each. The method of claim 1,
Centella extract further comprising the step of filtration after step e). A cosmetic composition for skin whitening comprising the centella extract of any one of claims 1 to 7. The method of claim 8,
The Centella extract is a cosmetic composition for skin whitening, characterized in that it inhibits tyrosinase activity and inhibits melanin production. delete

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