KR102001172B1 - Potable DNA Analyzer - Google Patents

Abstract

The present invention relates to a portable DNA analyzer comprising: a sample deck (100) provided in a case; a gene detection analysis module (200); an air injection module (300); a heating and cooling module (400); an image analysis and read module (500); and a control module (600) for automatically controlling the gene detection analysis module (200), the air injection module (300), the heating and cooling module (400), and the image analysis and read module (500), wherein the control module (600) comprises an air injection control unit (61), a motor control unit (62), a temperature control unit (63), an LED control unit (64), an electrophoresis control unit (65), and a power control unit (66). According to the present invention, the portable DNA analyzer can rapidly perform, in the field, a series of processes of extracting and amplifying a DNA genetic substance in a sample, separating a plurality of specific genes at once by using a capillary electrophoresis system, and reading the results from the separated images, through a single integrated device system. In addition, the portable DNA analyzer is configured to continuously perform gene amplification, electrophoresis, and analysis readout, thereby reducing a time required for the gene amplification, electrophoresis, and analysis readout.

Description

[0001] Portable DNA Analyzer [0002]

The present invention relates to a DNA analyzer, and more particularly, to a portable DNA analyzer configured to easily and easily perform a DNA analyzer in a real time where a DNA analysis is required.

In general, the PCR method is widely used for the analysis of DNA extracted from a subject. In order to proceed with such a PCR method, a thermal cycler device capable of amplifying the collected DNA and a control device for controlling the analysis of the collected DNA are required.

However, the real-time PCR apparatus using the conventional PCR method has been developed for a desktop and is very large in size, and is configured to operate in connection with an external computer for data processing, and the internal structure is designed to have low spatial efficiency. Therefore, it can be said that there are many ineffective and unnecessary parts to be used in actual field.

Particularly, in order to determine hereditary infectious diseases such as foot-and-mouth disease, analysis of the DNA of suspect animal in foot-and-mouth disease must be performed or the DNA must be moved to the place where the real-time PCR equipment is installed. There is a possibility of serious problem that it is difficult to manage the virus in accordance with the travel time.

In addition, there is a risk that a false positive result may be derived from an examiner or contamination, which is an inspection method capable of early diagnosis by confirming the presence or absence of a direct factor such as a genetic material of an infectious agent.

Recently, the necessity of on - site inspection for the diagnosis of infectious diseases has been emphasized in WHO, and on - site inspection systems using molecular biological methods have been developed steadily for about 10 years.

Furthermore, with the advent of lab-on-a-chip (LOC) technology that allows one system to perform all molecular diagnostic procedures, typically extraction of genetic material from a sample, gene amplification reaction, Research on possible high-efficiency and high-sensitivity systems is being actively pursued.

As a result of such research, a strip sensor module for a molecular diagnostic field inspection apparatus disclosed in Korean Patent No. 10-1767978, a genetic quantitative detection real time PCR and a gene analysis DNA microarray disclosed in Korean Patent No. 10-1302353 And a molecular diagnostic automatic analyzer that can be performed in a complex manner.

However, in the case of such a conventional molecular diagnostic field inspection apparatus, since the construction of a DNA chip and a measuring apparatus is generally complicated, the manufacturing cost of a product is high, and thus there is a disadvantage that the cost of a test use is high. In order to confirm the result of a PCR product Since there is a disadvantage in that the measurement reliability is lowered due to contamination in the transferring process due to transferring to a separate measuring device and initial determination is possible because gene extraction, amplification, separation and reading are not performed in one system, It takes a long time to obtain it and it is not suitable for on-site inspection.

In addition, real-time PCR has been developed for the on-site inspection. However, in Korea, the technology differs from the developed country in reality, and it is faced with limitations as a late buyer due to expensive equipment and reagents.

Korean Patent No. 10-1767978 Korean Patent No. 10-1302353

SUMMARY OF THE INVENTION The present invention has been made to solve the above-described problems, and an object of the present invention is to provide a method and apparatus for extracting and amplifying DNA genetic material from a specimen through a single integrated device system, and using a capillary electrophoresis apparatus, A portable DNA analyzing apparatus configured to perform a series of processes for separating a sample at one time and reading a result from a separated image in real time on the spot.

In order to solve the above-mentioned problems, the portable DNA analyzing apparatus according to the present invention comprises:

A sample deck 100 provided inside the case;

A gene detection and analysis module 200 which is seated and fixed on the sample deck 100, into which a DNA sample is injected for amplification and electrophoresis;

An air injection module 300 for supplying air into the DNA film chip 25 of the gene detection and analysis module 200 to discharge bubbles and an extra sample;

A heating and cooling module 400 for heating or cooling the gene amplification unit of the DNA film chip 25 of the gene detection and analysis module 200 from the top or bottom;

An image analysis reading module 500 for comparing and analyzing a plurality of linear images separated by the electrophoresis portion 25c of the gene detection and analysis module 200 with reference markers; And

An air injection control unit 61, a motor control unit 62, a temperature control unit 63, an LED control unit 64, an electrophoresis control unit 65 and a power source control unit 66. The gene detection analysis module 200 And a control module 600 for automatically controlling the air injection module 300, the heating and cooling module 400, and the image analysis reading module 500.

Further, in the portable DNA analyzer according to the present invention,

The sample deck (100)

A deck plate which is fixed to the inside of the front part of the case at one side and the deck plate which is connected to the deck sliding motor and slides in the back and forth direction, And a right groove having an electrophoretic contact portion on a bottom surface of the rectangular groove, the groove being divided into a left groove having a through groove and a right groove having an electrophoresis contact portion.

Further, in the portable DNA analyzer according to the present invention,

The gene detection and analysis module (200)

A top plate 20, a DNA film chip 25 and a bottom plate 27,

The upper plate (20)

A plate shape in which an air inlet 20a, a sample inlet 20b, a through hole 20c, a gel solution inlet 20d and a DNA collector 20e are sequentially formed from the upper end,

The DNA film chip (25)

The air and sample injecting section 25a, the gene amplifying section 25b and the electrophoresis section 25c are formed of microcapsules,

The lower plate (27)

A contact point to which electricity can be applied is provided at an upper end portion and a lower end portion so as to separate the nucleic acid of the amplified sample from the electrophoresis portion 25b and form an analysis read portion together with the upper plate 20,

The DNA film chip 25 is arranged to correspond to the rear surface of the upper plate 20 and the lower end of the film chip is coupled between the upper plate 20 and the lower plate 27 and is fixed by a fixing band .

Further, in the portable DNA analyzer according to the present invention,

The air injection module (300)

A syringe air pump 33 formed on the inner left side of the case and moved by a syringe drive motor 31 along a guide rail 32 fixed to the bottom of the case;

A support 34 fixedly connected to a support rod fixed to the bottom of the case; And

And is moved forward and backward by an air injection transfer motor 36 along a guide rod 35 rotatably fitted to the projecting arms 34a and 34b of the support stand 34. The syringe air pump 33, And an air block (37) connected to the air supply unit and having an air discharge nozzle (37a) formed therein.

Further, in the portable DNA analyzer according to the present invention,

The air block (37)

And the air discharge nozzle 37a is connected to the air inlet 20a through the plurality of air inlet ports 20a which are arranged adjacent to each other and move forward and backward along the guide rod to supply air through the nozzles.

Further, in the portable DNA analyzer according to the present invention,

The heating and cooling module (400)

And an upper heat-radiating portion 41 and a lower heat-radiating portion 42 facing each other,

The upper heat radiating part 41 and the lower heat radiating part 42 may be used alone or together at the same time.

Further, in the portable DNA analyzer according to the present invention,

The upper heat-radiating portion (41)

An upper heat sink 41b; An upper thermoelectric element 41c attached to the lower surface of the upper heat radiating plate 41b to heat or cool the gene amplifying unit disposed adjacent thereto; An upper heater cooling fan 41d provided on the upper heat radiating plate 41b; An upper press 41e provided on both sides of the upper heat radiating plate 41b and having a plurality of press tips at a lower portion thereof; And an upper heat transfer plate 41f attached to a lower surface of the upper thermoelectric element 41c,

The lower heat dissipation portion (42)

A lower heat sink 42b; A lower thermoelectric element 42c attached to the upper surface of the lower heat sink 42b to heat or cool the gene amplification unit disposed adjacent thereto; A lower heater cooling fan 42d provided below the lower heat sink 42b; And a lower heat transfer plate (42f) attached to the upper surface of the lower thermoelectric element (42c).

Further, in the portable DNA analyzer according to the present invention,

The upper heat-radiating portion (41)

And is connected to a support shaft fixed to the inner bottom of the case and fixed to the support plate 43 by the up / down drive motor 43a so as to be lifted and lowered together with the support plate 43. [

Further, in the portable DNA analyzer according to the present invention,

The upper thermoelectric element 41c and the lower thermoelectric element 42c,

A thermoelectric element that can raise or lower the heat to a predetermined temperature by a power source applied by the Peltier effect, or a planar heating element made of ceramic.

Further, in the portable DNA analyzer according to the present invention,

The upper press (41e)

The sample injection port 20b and the gel solution injection port 20d formed in the upper plate 20 of the gene detection and analysis module 200 are sealed and pressurized to prevent sample leakage during the amplification process.

Further, in the portable DNA analyzer according to the present invention,

The image analysis read module (500)

An imaging device (51) arranged to photograph an analysis reading part of the gene detection analysis module (200) which is seated in the right groove of the sample deck (100); And

And a light guide plate (52) provided below the groove on the right side of the sample deck (100) and serving as a lower illumination.

In addition, the portable DNA analyzing apparatus according to the present invention comprises:

A power supply terminal 700 for supplying power is further provided.

In addition, the portable DNA analyzing apparatus according to the present invention comprises:

The portable DNA analyzer 1 is configured to be capable of performing gene amplification, electrophoresis, and analysis reading continuously, thereby reducing the time required for amplification, the time required for electrophoresis, and the time required for analysis analysis.

The portable DNA analyzer according to the present invention extracts and amplifies a DNA genetic material from a specimen through an integrated unit system, separates a plurality of specific genes at one time using a capillary electrophoresis apparatus, In the field can be performed quickly in the field.

In addition, the portable DNA analyzer according to the present invention is capable of continuously performing gene amplification, electrophoresis, and analysis reading, thereby reducing the time required for amplification, the time required for electrophoresis, and the time required for analysis analysis.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a front elevation view showing a brief internal structure of a portable DNA analysis apparatus according to an embodiment of the present invention;
FIG. 2 is a plan view of the portable DNA analyzer 100 of FIG. 1,
FIG. 3 is a rear perspective view of the portable DNA analyzer 100 of FIG. 1,
FIG. 4 is a detailed perspective view of the air injection module of the portable DNA analyzer 100 of FIG. 1,
FIG. 5 is a schematic view illustrating a coupling structure of a heating and cooling module in a portable DNA analyzer according to an embodiment of the present invention. FIG.
FIG. 6 is a schematic view illustrating a structure of a heating and cooling module of a portable DNA analyzer according to an embodiment of the present invention;
7 is a view showing a role structure of a light guide plate and a camera of a portable DNA analyzer according to an embodiment of the present invention,
8A and 8B illustrate an example of a gene detection analysis module of a portable DNA analysis apparatus according to an embodiment of the present invention.
9 is a schematic internal configuration diagram of a portable DNA analyzer according to an embodiment of the present invention,
FIG. 10 is a view briefly showing the appearance of a portable DNA analyzer according to an embodiment of the present invention. FIG.

Hereinafter, preferred embodiments of the portable DNA analyzing apparatus of the present invention will be described in more detail with reference to the accompanying drawings.

2 is a plan view of the portable DNA analyzer 100 of FIG. 1; FIG. 3 is a top view of the portable DNA analyzer of FIG. 1; FIG. FIG. 4 is a detailed perspective view of an air injection module of the portable DNA analysis apparatus 100 of FIG. 1, and FIG. 5 is a perspective view of a portable DNA analysis apparatus according to an embodiment of the present invention. FIG. 6 is a schematic view illustrating a structure of a heating and cooling module of a portable DNA analyzer according to an embodiment of the present invention. FIG. 7 is a schematic view of a portable DNA analyzer according to an embodiment of the present invention. FIGS. 8A and 8B are views showing an example of a gene detection analysis module of a portable DNA analysis apparatus according to an embodiment of the present invention; FIGS. 8A and 8B are views showing role structures of a light guide plate and a camera of a DNA analysis apparatus; 9 is a portable D < RTI ID = 0.0 > FIG. 10 is a schematic view of an external appearance of a portable DNA analyzer according to an embodiment of the present invention. FIG.

1 and 10, a portable DNA analyzer 1 according to the present invention amplifies a DNA sample extracted from a collected specimen to a desired DNA region, and amplifies the amplified specimen in a gel solution (C); and a control unit for controlling the operation of the case (C); A sample deck 100; A gene detection analysis module 200; An air injection module 300; A heating and cooling module 400; An image analysis reading module 500 and a control module 600. [

Here, the case C has a rectangular shape, the front part OD is opened and closed by sliding, and one side of the sample deck 10 is fixedly coupled to the inner side of the front part. Of course, the present invention is not limited thereto and may be provided in various forms. In addition, the size of the case is preferably small so as to be portable, but is not limited in size.

The sample deck 100 is fixed on the inside of the front part of the case C and the other side is connected to the deck sliding motor 11 to slide in the forward and backward directions. 200 are arranged in parallel with the front surface of the case and are divided into a left groove having a through groove and a right groove having an electrophoresis contact portion on the bottom surface, The current is allowed to flow through the contact of the electrophoresis portion 25c, which will be described later.

The reason why the through hole is formed in the sample deck 100 is that the DNA film chip 25 of the gene detection and analysis module 200 located in the through groove can be heated and cooled in the vertical direction.

The gene detection and analysis module 200 comprises an upper plate 20, a DNA film chip 25 and a lower plate 27 through which the extracted DNA sample is charged and amplified and electrophoretically transferred.

The upper plate 20 is in the form of a transparent rectangular plate having a predetermined width and length and has an air inlet 20a, a sample inlet 20b, a through hole 20c, a gel solution inlet 20d and a DNA collector 20e, Are sequentially formed. Though the thickness of the upper plate is made to be approximately 3 mm, it is not limited thereto.

The DNA film chip 25 is formed such that the air and sample injecting section 25a, the gene amplifying section 25b and the linear electrophoresis section 25c are microcapsules. The DNA chip chip 25 is disposed on the rear surface of the upper plate, And the upper plate and the lower plate. The thickness of the film chip is approximately 430 μm, but it is not limited thereto.

The air and sample injecting section 25a is provided with an air injection hole and a sample injection hole communicating with the air injection port 20a and the sample injection port 20b of the upper plate 20 and supplied air is contained in the DNA film chip Allow residual bubbles and excess samples to be discharged, and allow DNA samples extracted from the specimen to be injected.

The gene amplification unit 25b amplifies a plurality of extracted DNAs with respect to a desired DNA region, and can be heated to a temperature controlled according to a DNA sample by a heat sink of the heating and cooling module 400. [

The linear electrophoresis unit 25c is formed by forming a plurality of microchip-based microcapsules based on injection molding and has a gel solution injection hole continuous to the gene amplification unit 25b and communicated with the gel solution injection port 20d. The nucleic acid of the DNA sample can be separated in the gel solution. At this time, the linear electrophoresis unit 25c includes a sample having DNA to be measured for reference and a size marker formed of a capillary of the same size equipped with a gel solution, To be separated under conditions.

By providing the size marker on the side surface and separating the sample together with the specimen sample in this way, there is no difference in analysis due to external environmental factors, separation and normal condition variation, and more accurate analysis and reading becomes possible.

Here, a polyacrylamide gel or an agarose gel is used as the gel solution, and the gel has functions of preventing convection, protecting the sample, and molecular sieving.

The lower plate 27 is in the form of a transparent rectangular plate having a predetermined width and a length and has contacts at the upper and lower ends to which electrical power can be applied to separate the nucleic acid of the amplified sample from the electrophoresis portion 25c by the applied electricity An analytical reading unit for comparing and analyzing a plurality of linear images separated by the linear electrophoresis unit 25c with reference markers can be formed so that comparative measurement is possible and the intervening film chips are fixed together with the upper plate .

The lower plate 27 has a length corresponding to the surface extending from the gel solution inlet 20d of the upper plate 20 to the lower end of the upper plate and the lower plate 27 has a thickness of about 5 mm However, the present invention is not limited thereto.

The fixed jointing band 28 fixes the upper plate and the lower plate and the rear portion of the film chip interposed therebetween from above the upper plate 20, and has a coupling protrusion formed inside thereof.

The coupling protrusion on the inner side not only enhances the coupling force between the upper plate and the lower plate and the film chip but also connects the gel solution inlet 20d to prevent the solution from leaking out during the process.

The air injection module 300 is provided on the inner left side of the case C for discharging residual bubbles and extra samples contained in the DNA film chip 25, A saddle air pump 33 which is moved along a guide rail 32 fixed to the floor of the main body C, a supporter 34 fixed to a fixed height of a support rod fixed to the floor, Is moved forward and backward by an air injection transfer motor (36) along a guide rod (35) rotatably fitted in a coupling hole formed in the protruding arms (34a, 34b) of the syringe air pump And an air block 37 which is supplied with air and has an air discharge nozzle 37a formed therein.

The air block 37 is rotated in the forward and backward directions along the guide rods so as to rotate at a plurality of adjacent air inlet ports 20a (for example, nine air ports) so that the air outlet nozzle 37a is connected to the air inlet 20a And supplies air through the nozzle.

The guide rails 32 and the guide rods 35 are arranged to be parallel to the upper end of the upper plate 20 of the gene detection and analysis module 200 so that the air discharge nozzle 37a of the air block 37 So that it can be precisely fitted to each of the plurality of air inlet ports 20a arranged adjacently in the turning movement.

The heating and cooling module 400 is an apparatus for heating and cooling the gene amplification unit 25b of the DNA film chip 25 of the gene detection and analysis module 200 from the upper side or the lower side to enhance the amplification efficiency, And a lower heat-radiating portion 42. The upper heat- Of course, the upper heat radiating portion 41 and the lower heat radiating portion 42 may be used alone or together at the same time.

The upper heat radiation portion 41 includes an upper cover 41a, an upper heat radiation plate 41b, an upper thermoelectric element 41c, an upper heater cooling fan 41d, an upper press 41e, and an upper heat transfer plate 41f, The lower heat radiation portion 42 includes a lower heat radiation plate 42b, a lower thermoelectric element 42c, a lower heater cooling fan 42d, and a lower heat transfer plate 42f. Of course, it is also possible to further include a lower cover (not shown) in the lower heat-radiating portion.

The upper cover 41a of the upper heat radiating portion 41 is connected to a support shaft fixed to the bottom of the inner right side of the case C and is vertically fixed to the support plate 43 by a vertical drive motor 43a So that the upper heat radiation portion 41 is raised and lowered together with the support plate 43.

The upper thermoelectric element 41c and the lower thermoelectric element 42c are attached to the lower surface and the upper surface of the upper heat radiating plate 41b and the lower heat radiating plate 42b to heat or cool the gene amplifying unit disposed adjacent thereto , A heat transfer element using a thermoelectric element or the like is arranged and the PCR is performed by the applied heat, so that the amplification time is greatly reduced. This is not a tubular type but a chamber type, so the surface area is widened to increase the PCR reaction speed and also to reduce the PCR time.

At this time, a thermoelectric element is provided and the temperature is raised to a predetermined temperature by a power source applied by the Peltier effect, and the temperature is maintained or lowered to provide a suitable temperature controlled according to the PCR cycle during the PCR. The heat transfer member is not limited to a thermoelectric element, and various surface heat emission elements may be applied. Further, a heat dissipation means may be further provided to lower or control the temperature.

The upper heat transfer plate 41f and the lower heat transfer plate 42f are attached to the lower surface of the upper thermoelectric element 41c and the upper surface of the lower thermoelectric element 42c to assist heat transfer. Do.

The upper press 41e is provided on the left and right sides of the upper heat radiating plate and is provided with a press tip having the same number of the sample injection port and the gel solution injection port on the lower edge thereof. 20 and the gel solution inlet 20d are sealed and pressurized to prevent the sample or the like from flowing out during the amplification process.

The upper heater cooling fan 41d and the lower heater cooling fan 42d are provided at upper and lower portions of the upper heat radiating plate 41b and the lower heat radiating plate 42b to rapidly diffuse the heat transmitted to the heat radiating plate will be.

The image analysis reading module 500 separates the nucleic acid of the amplified sample from the electrophoresis portion 25b so as to enable comparison measurement and compares the plurality of linear images separated by the electrophoresis portion 25c with the reference marker, An imaging device 51 fixed to the support plate 43 to photograph the analysis reading section of the gene detection analysis module 200 placed in the right groove of the sample deck 100, And an ultraviolet light guide plate 52 provided at the lower surface of the light guide plate 52 and serving as a lower illumination. The light guide plate 52 may include a blue LED or an ultraviolet LED. The pyramidal pyramid shape of Fig. 7 and Fig. 1 shows the angle of view (photographing area) of the image pickup apparatus.

The control module 600 includes an air injection control section 61, a motor control section 62, a temperature control section 63, an LED control section 64, an electrophoresis control section 65 and a power source control section 66, The gene detection and analysis module 200, the air injection module 300, the heating and cooling module 400, and the image analysis reading module 500 are automatically controlled and provided to the monitoring S / Display.

A power terminal 700 connected to an external power supply unit (not shown) supplies power to the components.

A portable DNA analyzer (1) according to the present invention is a portable DNA analyzer (1) which can be easily moved at any time and anywhere, amplifies a DNA sample extracted from a collected sample to a desired DNA region, and amplifies the sample according to a voltage And the process of analyzing and reading the separated sample images can be continuously processed in real time.

Hereinafter, the operation of the portable DNA analyzer 1 according to the present invention will be described.

First, as a method for extracting a gene from a specimen, a conventional one can be used. In order to extract DNA more efficiently, a system for extracting DNA from blood is provided with a mesh structure coated with a reagent capable of DNA binding One extractor can be constructed.

Exposing the chromosome of the blood extracted from the specimen and destroying the protein and RNA; Introducing an extractor having a mesh structure into blood in which protein and RNA are broken, and aggregating DNA; Washing the extractor with the DNA aggregated; And separating the DNA from the extractor in which the washed DNA is aggregated.

When the front door of the case C is pulled from the outside, the sample deck fixed to the inside of the door comes out along the door by the sliding drive motor.

When the case door is closed, the air block of the air injection module moves forward along the guide rod by the feed motor and rotates at the position of the first air inlet port so that the air discharge nozzle And is connected to the corresponding air injection port.

When the air supply to the first air inlet is completed, the air block is again rotated back to its original position and then advanced to supply air to the adjacent air inlet in the same manner. In this manner, air is supplied to each air inlet port to remove moisture and remove excess sample.

Then, the prepared DNA sample is injected into the capillary through a sample injection port (not shown) or through a dispenser or through activation of an injection operation button through a sample injection port.

When the injected DNA sample rapidly moves to the gene amplification part by the capillary phenomenon, the heating and cooling module is lowered or raised so that the press tip of the upper press seals the sample injection port and the gel solution injection port so that the sample is not leaked And the upper heat transfer plate provided on the lower surface of the heating and cooling module or the lower heat transfer plate provided on the upper surface heat and cool the gene amplification unit.

The sample amplified through the heating and cooling module is injected into the capillary tube of the electrophoresis portion 25c connected to the respective capillaries, and the gel solution is injected through the gel solution injection port.

Next, when a DC voltage is applied to the electrophoresis portion, the charged DNA sample moves from the charged gel, and the amplified DNA sample is separated and transferred by the charged particles. This is due to the movement of the charged particles and the moving speed of the particles depending on the molecular weight. In this case, the gel solution and the gene are rapidly moved forward due to the capillary phenomenon due to the capillary structure based on the microchip, and therefore the analysis is possible within a short time since the moving speed is fast and the probe is rapidly moved and diffused.

Next, a plurality of linear images measured from the electrophoresis section are photographed using an imaging device and an LED light of an image analysis read module, analyzed and read with a reference marker through an internal analysis program, analyzed and read by the analysis reader The resultant value may be displayed on a display device or a separate play means.

In such a compact and portable DNA analyzer, since the gene amplification unit, the electrophoresis unit, and the analysis read unit are integrated, the sample can be automatically injected into the electrophoresis pad after the PCR is performed, and the PCR separation can be performed simultaneously, It becomes possible to avoid the inconvenience of moving the PCR amplification substance and to prevent the contamination during the migration process. In addition, since the time required from gene amplification to analytical reading is greatly reduced, it is relatively easy to conduct researches, research institutes, local governments, Immediate inspection is possible.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

1: DNA analyzer
100: Sample deck
10: Deck plate 11: Deck sliding motor
200: gene detection analysis module
20: upper plate 20a: air inlet
20b: sample inlet 20c: through hole
20d: Gel solution inlet 20e: DNA collector
25: DNA film chip 25a: air and sample injection part
25b: gene amplification unit 25c: linear electrophoresis unit
27: lower plate 28:
300: air injection module
31: Syringe drive motor 32: Guide rail
33: syringe air pump 34: support
34a, 34b: protruding arm 35: guide rod
36: Air injection feed motor 37: Air block
37a: Air discharge nozzle
400: Heating and cooling module
41: upper heat radiating part 42: lower heat radiating part
41a: upper cover 41b: upper heat sink
41c: upper thermoelectric element 41d: upper heater cooling fan
41e: upper press 41f: upper heat transfer plate
42b: lower heat sink 42c: lower thermoelectric element
42d: lower heater cooling fan 42f: lower heat transfer plate
500: Image analysis read module
51: imaging device 52: light guide plate
600: control module
61: air injection control section 62: motor control section
63: temperature control unit 64: LED control unit
65: electrophoresis control unit 66: power control unit
700: Power terminal C: Case
P: Cooling fan t: Press tip

Claims (13)

A sample deck 100 provided inside the case;
A gene detection and analysis module 200 which is seated and fixed on the sample deck 100, into which a DNA sample is injected for amplification and electrophoresis;
An air injection module 300 for supplying air into the DNA film chip 25 of the gene detection and analysis module 200 to discharge bubbles and an extra sample;
A heating and cooling module 400 for heating or cooling the gene amplification unit of the DNA film chip 25 of the gene detection and analysis module 200 from the top or bottom;
An image analysis reading module 500 for comparing and analyzing a plurality of linear images separated by the electrophoresis portion 25c of the gene detection and analysis module 200 with reference markers; And
An air injection control unit 61, a temperature control unit 63, an electrophoresis control unit 65 and a power source control unit 66. The gene detection and analysis module 200, the air injection module 300, And a control module (600) for automatically controlling the cooling analysis module (400) and the image analysis reading module (500).
The apparatus of claim 1, wherein the sample deck (100)
A deck plate which is fixed to the inside of the front part of the case at one side and the deck plate which is connected to the deck sliding motor and slides in the back and forth direction, Wherein a rectangular groove having a through groove is formed in parallel with the front surface of the case in the longitudinal direction and is divided into a left groove having a through groove and a right groove having an electrophoresis contact portion on the bottom surface.
2. The method of claim 1, wherein the gene detection analysis module (200)
A top plate 20, a DNA film chip 25 and a bottom plate 27,
The upper plate (20)
A plate shape in which an air inlet 20a, a sample inlet 20b, a through hole 20c, a gel solution inlet 20d and a DNA collector 20e are sequentially formed from the upper end,
The DNA film chip (25)
The air and sample injecting section 25a, the gene amplifying section 25b and the electrophoresis section 25c are formed of microcapsules,
The lower plate (27)
A contact point to which electricity can be applied is provided at an upper end portion and a lower end portion so as to separate the nucleic acid of the amplified sample from the electrophoresis portion 25b and form an analysis read portion together with the upper plate 20,
The DNA film chip 25 is arranged to correspond to the rear surface of the upper plate 20 and the lower end of the film chip is coupled between the upper plate 20 and the lower plate 27, Portable DNA analyzer.
The air injection module according to claim 1, wherein the air injection module (300)
A syringe air pump 33 formed on the inner left side of the case and moved by a syringe drive motor 31 along a guide rail 32 fixed to the bottom of the case;
A support 34 fixedly connected to a support rod fixed to the bottom of the case; And
And is moved forward and backward by an air injection transfer motor 36 along a guide rod 35 rotatably fitted to the projecting arms 34a and 34b of the support stand 34. The syringe air pump 33, And an air block (37) to which air is supplied and an air discharge nozzle (37a) is formed.
The airbag (37) according to claim 4, wherein the airblock (37)
(20a) that are moved in the forward and backward directions along the guide rods, respectively, to connect the air discharge nozzle (37a) to the air inlet (20a) and to supply air through the nozzles DNA analyzer.
The method of claim 1, wherein the heating and cooling module (400)
And an upper heat-radiating portion 41 and a lower heat-radiating portion 42 facing each other,
The portable DNA analyzer according to any one of the preceding claims, wherein the upper and lower radiating parts (41, 42) can be used singly or simultaneously.
The method according to claim 6,
The upper heat-radiating portion (41)
An upper heat sink 41b; An upper thermoelectric element 41c attached to the lower surface of the upper heat radiating plate 41b to heat or cool the gene amplifying unit disposed adjacent thereto; An upper heater cooling fan 41d provided on the upper heat radiating plate 41b; An upper press 41e provided on both sides of the upper heat radiating plate 41b and having a plurality of press tips at a lower portion thereof; And an upper heat transfer plate 41f attached to a lower surface of the upper thermoelectric element 41c,
The lower heat dissipation portion (42)
A lower heat sink 42b; A lower thermoelectric element 42c attached to the upper surface of the lower heat sink 42b to heat or cool the gene amplification unit disposed adjacent thereto; A lower heater cooling fan 42d provided below the lower heat sink 42b; And a lower heat transfer plate (42f) attached to an upper surface of the lower thermoelectric element (42c).
8. The heat sink according to claim 7, wherein the upper heat-
Is connected to a support shaft fixed to an inner bottom of the case and is fixedly coupled to and supported by a support plate (43) by means of a vertical drive motor (43a), and moves up and down together with the support plate (43) .
8. The method of claim 7, wherein the upper thermoelectric element (41c) and the lower thermoelectric element (42c)
Wherein the thermoelectric element is a thermoelectric element capable of raising or lowering heat to a predetermined temperature by a power source applied by a Peltier effect, or a surface heating element made of ceramic.
8. The apparatus according to claim 7, wherein the upper press (41e)
Wherein the sample injection port (20b) and the gel solution injection port (20d) formed in the upper plate (20) of the gene detection and analysis module (200) are sealed and pressurized to prevent sample leakage during the amplification process.
The apparatus of claim 1, wherein the image analysis reading module (500)
An imaging device (51) arranged to photograph an analysis reading part of the gene detection analysis module (200) which is seated in the right groove of the sample deck (100); And
And a light guide plate (52) provided below the groove on the right side of the sample deck (100) and serving as a lower illumination unit.
12. The method according to any one of claims 1 to 11,
And a power terminal (700) for supplying power to the portable DNA analyzer.
12. The method according to any one of claims 1 to 11,
The portable DNA analyzer (1) is configured to be capable of performing gene amplification, electrophoresis, and analysis reading continuously, thereby reducing the time required for amplification, the time required for electrophoresis, Analysis device.

Images