KR101997187B1 - A composition for identifying of mycobacterium and specimen container - Google Patents

Abstract

Provided are a composition for use in detecting mycobacterium and a specimen container comprising the same. The composition used for the mycobacterium detection includes sodium hydroxide, sodium hypochlorite, sodium citrate, and a solvent.

Description

A composition used to detect mycobacterium and a sample container comprising the same {A COMPOSITION FOR IDENTIFYING OF MYCOBACTERIUM AND SPECIMEN CONTAINER}

The present invention relates to a composition used for detecting mycobacterium and a sample container comprising the same, and more particularly, to a composition and a mycobacter used for detecting Mycobacterium tuberculosis in human sputum. It relates to a sample container used for detection of Leeum tuberculosis.

Tuberculosis is an infectious disease which is infected with a Mycobacterium (Mycobacterium) organisms expressing latent, progressive, activity. Specifically, the causative agents of tuberculosis ( mycobacterium tuberculosis ) include mycobacterium tuberculosis , mycobacterium bovis , and mycobacterium avium . Mycobacterium tuberculosis is the main causative agent. Mycobacterium tuberculosis is rod-shaped with 0.2-0.5 micrometer in thickness and 1-4 micrometers in length. Mycobacterium tuberculosis is an antioxidant bacterium that is long-lived in dry conditions and is resistant to strong acids and alkalis.

Tuberculosis is mainly transmitted from person to person through the air. When an infectious tuberculosis patient speaks, coughs, or sneezes, it is spread in the air in the form of droplets containing the tuberculosis bacteria. At this time, the scattered tuberculosis bacteria invade into the lungs of people around them to multiply and become infected.

Commonly used tests for the diagnosis of tuberculosis may include skin test, anti-acid staining and culture through smear, and chest X-ray.

Republic of Korea Patent No. 10-1129806

Among the tests used for the diagnosis of tuberculosis, the microbial smear test is a method in which a tube, such as a human sputum, is passed through a filter to remove impurities, and then thinly coated on a slide to selectively stain only tuberculosis bacteria and observed with a microscope. Collective smear test is a simple staining method for bacteriological diagnosis. As a result, infection can be quickly and easily detected and treated as active tuberculosis patients since it is relatively simple and quick to check for infection.

However, since the smear test performs a smear test by taking a sample from a liquid sample suspected of having M. tuberculosis, the inspector such as a clinician or a pathologist who carries out the test is always at risk of exposure to M. tuberculosis. For example, in the process of moving a sample provided from a patient, filtering a sample, collecting a sample, smearing a sample, or observing a slide on which a sample is smeared, the tuberculosis bacteria are propagated into the air, and the inspector is transmitted to the tuberculosis bacteria. There is a risk of infection.

Therefore, the development of a new test method for the relatively simple and rapid while suppressing the infection of Mycobacterium tuberculosis is required.

The problem to be solved by the present invention is to provide a composition used for the detection of mycobacterium that can suppress the infection of Mycobacterium tuberculosis.

Another problem to be solved by the present invention is to provide a sample container used for the detection of mycobacterium that can suppress the infection of Mycobacterium tuberculosis.

The objects of the present invention are not limited to the above-mentioned technical problem, and other technical problems not mentioned will be clearly understood by those skilled in the art from the following description.

The composition according to an embodiment of the present invention for solving the above problems, sodium hydroxide (sodium hydroxide), sodium hypochlorite (sodium hypochlorite), sodium citrate (sodium citrate), and a solvent.

The composition may include 110 to 180 parts by weight of sodium hypochlorite, and 60 to 90 parts by weight of sodium citrate, based on 100 parts by weight of sodium hydroxide.

In addition, the composition may include 3.5 wt% to 4.0 wt% of the sodium hydroxide, 4.5 wt% to 6.0 wt% of the sodium hypochlorite, and 2.4 wt% to 3.0 wt% of the sodium citrate.

In addition, the solvent is distilled water, the composition may include the sodium hydroxide, the sodium hypochlorite, the sodium citrate and 87.0% to 89.6% by weight of the distilled water.

The composition can be used to eliminate the infectivity of mycobacterium.

The sample container according to an embodiment of the present invention for solving the other problem is a first inner space sealed and accommodated with a composition comprising sodium hydroxide, sodium hypochlorite, sodium citrate and a solvent, and the first inner space and A second internal space configured to be detachable and openable.

The sample container may include a cap part having the first internal space, and a sample accommodating part coupled to the cap part to form the second internal space.

In addition, the cap portion has an annular fastening body having a coupling means that can be coupled to the sample receiving portion, the pressing portion disposed on the upper side of the inner peripheral surface of the annular fastening body, the side wall portion protruding downward from the pressing edge; A cutout portion protruding downward from the center of the pressing portion and inserted into the sidewall portion, and a sealing sheet disposed at a lower end of the sidewall portion and forming the first internal space together with the pressing portion and the sidewall portion. Can be.

The specimen container may be configured such that when the pressing portion moves downward, the cutting portion protruding from the pressing portion cuts the sealing sheet so that the composition contained in the first inner space moves to the second inner space side.

Specific details of other embodiments are included in the detailed description.

The composition according to an embodiment of the present invention can prevent the M. tuberculosis infection of the tester who performs the smear by selectively removing the infectivity of M. tuberculosis.

In addition, the specimen container according to an embodiment of the present invention can be completely sealed to the specimen suspected of having the tuberculosis bacteria can be prevented in advance to prevent the risk of transmission of the tuberculosis bacteria in the process of moving the specimen provided from the patient, By storing the composition in a separate space and selectively killing only infectious tuberculosis bacteria, the tuberculosis infection of the examiner can be prevented by opening the package immediately before smearing.

Effects according to embodiments of the present invention are not limited by the contents exemplified above, and more various effects are included in the present specification.

1 is a perspective view of a specimen container according to an embodiment of the present invention.
FIG. 2 is an exploded perspective view of the specimen container of FIG. 1. FIG.
3 is a cross-sectional perspective view of the specimen container of FIG. 1.
4 is a cross-sectional view of the specimen container of FIG. 1.
5 is a perspective view of the slit portion of FIG. 2.
6 is a cross-sectional view for explaining the operation of the specimen container of FIG.

Advantages and features of the present invention and methods for achieving them will be apparent with reference to the embodiments described below in detail with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various forms, and only the embodiments are provided to make the disclosure of the present invention complete, and those of ordinary skill in the art to which the present invention pertains. It is provided to fully inform the scope of the invention, and the invention is defined only by the scope of the claims.

As used herein, the term 'tuberculosis' refers to an infectious disease caused by infection by the Mycobacterium complex including Mycobacterium tuberculosis bacteria, bovine tuberculosis bacteria, and tuberculosis tuberculosis.

As used herein, the term 'to' refers to a numerical range including a numerical value described before and a numerical value described later as a lower limit and an upper limit, respectively.

Hereinafter, a composition used for detecting mycobacterium according to the present invention and a sample container including the same will be described in detail.

The composition used for detecting mycobacterium according to an embodiment of the present invention includes sodium hydroxide (NaOH), sodium hypochlorite (NaOCl), sodium citrate, and a residual amount of solvent. .

The sodium hydroxide acts as an emulsifier and decontamination agent so that when the sample contains a mucus material such as sputum, it can decompose the mucus material and inhibit the growth of bacteria. In addition, the viscosity of the sample can be lowered by decomposing the mucous substance (for example, mucin), thereby facilitating smearing of the sample on the slide, thereby obtaining a clearer image. In addition, as the viscosity of the sample is lowered, the filtering characteristics of the sample can be improved. On the other hand, if the content of sodium hydroxide is too small, the decomposition ability of the mucus material may be significantly reduced.

The sodium hypochlorite may have a selective killing or bactericidal action against mycobacterium. For example, hypochlorite (HClO), which occurs when sodium hypochlorite is diluted in a solvent (such as water), penetrates the cell membrane of mycobacterium to remove enzymatic action, and when sodium hypochlorite is diluted in the solvent. The generated oxygen (O) can maximize the induction of death by oxidizing the cell membrane.

In an exemplary embodiment, the content of sodium hypochlorite may be greater than the content of sodium hydroxide. For example, the upper limit of the content of sodium hypochlorite may be about 180 parts by weight, or about 170 parts by weight, or about 150 parts by weight based on 100 parts by weight of sodium hydroxide. In addition, the lower limit of the content of sodium hypochlorite may be about 110 parts by weight based on 100 parts by weight of sodium hydroxide. If the sodium hypochlorite is included in less than 110 parts by weight may lack the killing characteristics for mycobacterium. In addition, staining and smearing of samples may affect staining characteristics. If the sodium hypochlorite is included in more than 180 parts by weight, precipitates may occur during long-term storage of the composition may inhibit storage stability. In addition, the staining characteristics of the smeared sample may be degraded by destroying other bacteria or background cells other than Mycobacterium in the sample.

The sodium citrate chelates heavy metal ions in a sample, such as sputum, to promote the functionality of the sputum degrading enzyme and contribute to stabilization.

In an exemplary embodiment, the content of sodium citrate may be less than the content of sodium hydroxide. For example, the upper limit of the content of sodium citrate may be about 90 parts by weight, or about 80 parts by weight based on 100 parts by weight of sodium hydroxide. In addition, the lower limit of the content of sodium citrate may be about 60 parts by weight, or about 65 parts by weight based on 100 parts by weight of sodium hydroxide. If the sodium citrate is included in less than 60 parts by weight it may be difficult to completely chelate between heavy metal ions in the sample. In addition, when the sodium citrate is included in more than 90 parts by weight can reduce the killing characteristics of the sodium hypochlorite.

The solvent is not particularly limited as long as it is a substance capable of dissolving sodium hydroxide, sodium hypochlorite and sodium citrate, but may be, for example, distilled water or deionized water.

As a non-limiting example, the composition used for detecting mycobacterium according to the present embodiment is about 3.5% to 4.0% by weight sodium hydroxide, about 4.5% to 6.0% by weight sodium hypochlorite, and sodium citrate based on the total weight. And 2.4% to 3.0% by weight and the balance of the solvent. For example, the solvent is distilled water and the composition may be composed of only sodium hydroxide, sodium hypochlorite, sodium citrate and 87.0% to 89.6% by weight of the distilled water.

The composition according to the present embodiment can selectively kill only bacteria belonging to the mycobacterium group. For example, the composition according to the present embodiment may selectively kill only mycobacterium and not kill other bacteria when contacted with mycobacterium in human sputum, but the present invention is not limited thereto. As used herein, the term 'kill' means to maintain the unique shape of the target bacteria to be killed by visually identifiable, but to remove the infectivity of the target bacteria.

In addition, when the sample has a viscosity including mucus material like human sputum, when filtering the sputum as necessary, there is a problem that the filter is blocked or it is difficult to apply the sputum to the slide thinly. In addition, because the sputum has a viscosity, the surface of the smear is uneven and uneven. However, the composition according to the present embodiment may be mixed with sputum to lower the viscosity by decomposing mucin in sputum, thereby improving workability and having an effect of evenly forming a smear surface when smeared on a slide.

Hereinafter, the experimental example of this invention is demonstrated.

[Production example]

A composition was prepared comprising 4.0 wt% sodium hydroxide (NaOH), 2.9 wt% sodium citrate, 3.0 wt% sodium hypochlorite (NaOCl) and the balance of distilled water.

Comparative Example 1

The composition was prepared in the same manner as in the preparation example, except that the content of sodium hypochlorite was 6.0 wt% and the remaining amount of distilled water was included.

Comparative Example 2

The composition was prepared in the same manner as in Preparation Example, except that the content of sodium hypochlorite was 9.0 wt% and the remaining amount of distilled water was included.

Experimental Example

The viscosity and filtering characteristics of the compositions according to Preparation Examples and Comparative Examples 1 and 2 were evaluated and the results are shown in Table 1 below. In Table 1 below, ○ means low viscosity or excellent filtering characteristics, × means high viscosity or poor filtering characteristics, and Δ means the middle thereof.

In addition, the compositions according to Preparation Examples and Comparative Examples 1 and 2 were mixed with sputum of tuberculosis patients, respectively, and the mixture was smeared on a slide and stained. The results are shown in Table 1 below. In Table 1 below, ○ indicates excellent staining state, and easy detection of Mycobacterium tuberculosis, × means poor staining state, and mycobacterium tuberculosis detection is not easy, and △ means the middle thereof.

Composition Characterization Dyeing characteristic evaluation Viscosity Filtering properties Background cell state after smearing Image rating Production Example ○ △

○ Comparative Example 1 △ ×

△ Comparative Example 2 × ○

×

Hereinafter, with reference to the accompanying drawings will be described in detail with respect to the specimen container according to an embodiment of the present invention.

1 is a perspective view of a specimen container according to an embodiment of the present invention. FIG. 2 is an exploded perspective view of the specimen container of FIG. 1. FIG. 3 is a cross-sectional perspective view of the specimen container of FIG. 1. 4 is a cross-sectional view of the specimen container of FIG. 1.

1 to 4, the specimen container 1000 according to the present embodiment includes a cap part 100 and a cylindrical sample accommodating part 200 coupled to the cap part 100, so that the first internal space is sealed. And a second internal space S2 configured to be openable and closed. The specimen container 1000 may be a container used for storing and storing the specimen and killing mycobacterium.

The cap part 100 may have a first internal space S1 in which the composition CP used for detecting mycobacterium may be accommodated. In detail, the cap part 100 includes an annular fastening body 110 and a pressing part 120 disposed on an upper side of the inner circumferential surface of the annular fastening body 110 and a side wall part protruding downward from an edge of the pressing part 120. 130, a cutout portion 140 protruding downward from the center of the pressing part 120, and a sealing sheet 150 disposed at a lower end of the sidewall part 130. In a state where the cap part 100 and the sample accommodating part 200 are fastened, the cap part 100 may be located at least partially inside the sample accommodating part 200.

The fastening body 110 may be a portion that is in contact with the specimen receiving part 200. For example, the annular fastening body 110 may have a reversible coupling means that can be coupled with the sample receiving portion 200. As used herein, the term 'reversible coupling means' means a coupling means that can be repeatedly engaged and released.

In an exemplary embodiment, the annular fastening body 110 may have a first thread 115 formed on the lower side of the inner circumferential surface and may be screwed with the second thread 205 of the upper outer circumferential surface of the sample accommodating part 200. . However, the present invention is not limited thereto, and in another embodiment, the fastening body 110 and the sample accommodating part 200 may be clip-coupled or slide-coupled.

The pressing unit 120 may be disposed in a circular shape on the upper side of the inner circumferential surface of the fastening body 110 to form a cover forming an upper end of the cap unit 100. In addition, the pressing unit 120 may be configured to deform the shape when an external force is applied. In an exemplary embodiment, the pressing portion 120 may include an elastic dome 125 positioned approximately in the center of the plane. The elastic dome 125 may have a convex upward shape so that the center is higher than the edge. When downward pressure is applied to the elastic dome 125, the pressing part 120 may be movable downward as a whole. In some embodiments, when the external force is removed, the elastic dome 125 may be restored to its original shape by the elastic force. In this case, in order to increase the restoring force of the elastic dome 125, the pressing part 120 may further include a restoring rib.

The side wall part 130 may protrude downward from the edge of the pressing part 120 to form a side wall of the cap part 100. The side wall part 130 may be inserted into the sample accommodating part 200. An upper end of the side wall part 130 may be connected to the pressing part 120. The cross section of the side wall portion 130 may have an annular shape, and the side wall portion 130 may have an approximately cylindrical shape with an open lower end. In this case, the outer diameter of the side wall portion 130 may be smaller than the inner diameter of the fastening body 110. That is, the outer wall of the side wall portion 130 may be spaced inward from the inner peripheral surface of the annular fastening body 110.

The cutout 140 may protrude downward from the center of the pressing part 120 and be inserted into the sidewall part 130. An upper end of the cutout 140 may be connected to the pressing part 120, and a lower end of the cutout 140 may be located above the sealing sheet 150. In the drawings, the lower end of the cutout 140 and the upper surface of the sealing sheet 150 are in contact with each other. However, in another embodiment, the lower end of the cutout 140 is spaced upward from the upper surface of the sealing sheet 150. May be When the pressing unit 120 moves downward by an external force, the cutout unit 140 connected to the pressing unit 120 may move downward with the pressing unit 120. Through this, the cutout 140 may cut a substantially central portion of the sealing sheet 150.

The sealing sheet 150 may be disposed at the lower end of the side wall part 130 to form a cover that forms the lower end of the cap part 100. The sealing sheet 150 may be made of a material that does not penetrate the liquid composition. For example, the sealing sheet 150 may be made of silver foil or a synthetic resin thin film. The sealing sheet 150 may form the first internal space S1 of the cap part 100 together with the pressing part 120 and the side wall part 130.

In the first internal space S1, the composition CP according to an embodiment of the present invention described above may be accommodated and sealed. In an exemplary embodiment, the composition (CP) may be a composition used for mycobacterium detection, including sodium hydroxide, sodium hypochlorite, sodium citrate and a solvent. For example, the composition (CP) may be a composition that selectively kills mycobacterium. Since the composition (CP) has been described in detail above, a detailed description thereof will be omitted.

The sample receiving part 200 may be a cylindrical container having an open top. The sample receiving unit 200 may provide a space in which a sample, for example, a human sputum is stored. The sample accommodating part 200 may have a reversible coupling means that may be coupled to the cap part 100, specifically, the fastening body 110 of the cap part 100. In an exemplary embodiment, the sample receiving part 200 may be screwed with the first thread 115 formed on the lower side of the inner peripheral surface of the fastening body 110 with the second thread 205 formed on the upper outer peripheral surface. In another embodiment, the sample receiving portion 200 and the fastening body 110 may be clip coupled or slide coupled.

The specimen accommodating part 200 may have a locking step 215 disposed below the inner circumferential surface. The locking jaw 215 may protrude from the inner wall of the sample accommodating part 200 to form a step. At least a portion of the side wall 130 and the cutout 140 and the sealing sheet 150 may be positioned in the sample accommodating part 200 in a state in which the cap part 100 and the sample accommodating part 200 are fastened. In addition, the inner wall of the sample accommodating part 200, the lower surface of the pressing part 120, the outer wall of the side wall part 130, and the lower surface of the sealing sheet 150 form a second internal space S2 of the specimen container 1000. Can be formed. A specimen (not shown) may be stored in the second internal space S2 separated from the first internal space S1. The second internal space S2 may be configured to be opened and closed by fastening and releasing between the sample accommodating part 200 and the fastening body 110.

In some embodiments, the specimen container 1000 may further include a slit portion 300 coupled with the lower portion of the cap portion 100 and positioned within the sample receiving portion 200. The slit part 300 may be located on a path through which the composition CP accommodated in the first internal space S1 moves toward the second internal space S2. In a state where the cap part 100 and the sample accommodating part 200 are fastened, the locking jaw 215 may be in contact with the slit part 300 to stably support the slit part 300.

Hereinafter, the slit part 300 will be described in detail with reference to FIG. 5. 5 is a perspective view of the slit portion of FIG. 2.

1 to 5, the slit part 300 protrudes from the lower side of the inner circumferential surface of the annular slit body 310 and the slit body 310 having an inner diameter larger than the outer diameter of the side wall part 130 of the cap part 100. First slits 325 formed substantially radially on the first plate 320, and protruding further downward from an inner edge of the first plate 320 to be substantially radially formed on the second plate 330 having a step. It may include a plurality of partitions 340 formed between the two slits 335 and the first plate 320 and the second plate 330 to protrude upward.

The slit body 310 may be a portion that is in contact with the cap 100. For example, the annular slit body 310 may be reversibly coupled to the lower end of the side wall portion 130 of the cap portion 100. In an exemplary embodiment, the inner circumferential surface of the slit body 310 and the outer circumferential surface of the side wall portion 130 may be fitted. However, the present invention is not limited thereto, and in another embodiment, the slit body 310 and the sidewall 130 may be screwed, clipped, or slide coupled.

The first plate 320 may be annularly disposed on the lower side of the inner circumferential surface of the slit body 310. First slits 325 may be formed substantially radially in the first plate 320 to provide a path through which the composition CP and the air move.

In the state where the cap part 100 and the sample receiving part 200 are coupled, the locking jaw 215 of the sample receiving part 200 may be in contact with the slit body 310 and the first plate 320 at least partially. have. That is, the height of the locking jaw 215 is formed at the same level as the lower surface of the first plate 320 can stably support the slit 300.

The second plate 330 may protrude downward from the inner edge of the first plate 320 and may be disposed in a circular shape. The second plate 330 may have a step with the first plate 320. The upper surface of the second plate 330 may be located below the lower end of the cutout 140 when the cutout 140 is moved to the lower side as much as possible. Second slits 335 may be formed substantially radially in the second plate 330 to provide a path through which the composition CP and air move. The lower surface of the second plate 330 may be located at a level lower than the height of the locking step 215.

A plurality of partitions 340 may be disposed between the first plate 320 and the second plate 330. The plurality of partitions 340 are spaced apart from each other to partially partition the space above the first plate 320 and the space above the second plate 330, and the space above the first plate 320 and the second plate. The spaces above the 330 may be in fluid communication with each other. At the same time, the partition 340 may serve to guide the movement of the cutout 140 when the cutout 140 moves downward.

In an exemplary embodiment, the second slits 335 located at the center side relative to the first slits 325 move the composition CP from the first inner space S1 to the second inner space S2. Can provide a primary path. In this case, the second slits 335 may provide a path for air in the second internal space S2 to move to the first internal space S1. As a result, the first slits 325 and the second slits 335 having a small size may be prevented from being blocked by the composition CP. In addition, when the composition CP moves beyond the passage provided by the first slits 325, a portion of the overflowed composition CP may pass through the second slits 335. That is, the second slits 335 may provide a secondary path through which the composition CP moves from the first internal space S1 to the second internal space S2.

In addition, for example, when the sample stored in the sample accommodating part 200 has a viscosity like human sputum, the sputum and the composition (CP) may not be completely mixed even when contacted with the mycobacterium. have. However, the sputum and composition CP may be more easily dispersed and mixed by the plurality of first slits 325 and the second slits 335 formed in the slit part 300 and the mycobacterium in the sputum. It can be expected that this will be completely killed and the contagion removed.

Hereinafter, the operation of the specimen container 1000 according to the present embodiment will be described in detail with reference to FIG. 6. 6 is a cross-sectional view for explaining the operation of the specimen container of FIG.

1 to 6, when downward pressure is applied to the pressing part 120 of the specimen container 1000, the cutout part 140 connected to the elastic dome 125 and the pressing part 120 of the pressing part 120 are provided. Moves downwards. The lower end of the cutout 140 moved downward may partially cut a substantially central portion of the sealing sheet 150.

The composition CP contained in the first internal space S1 of the cap part 100, specifically, the first internal space S1 formed by the side wall part 130, the pressing part 120, and the sealing sheet 150. ) May move downward while the sealing sheet 150 is partially cut away. Specifically, the composition CP accommodated in the first internal space S1 passes through the slit part 300 to the second internal space S2 in the sample accommodating part 200 in which the sample SP is stored. I can move it. The sample SP is in contact with the composition CP in the second internal space S2 so that the mycobacterium of the sample SP may be selectively killed.

The specimen container 1000 according to the present embodiment is tightly coupled with the cap part 100 in a state in which the specimen SP is accommodated in the specimen accommodating part 200 to form a second internal space S2 in which contact with the outside is blocked. can do. That is, the sample SP accommodated in the second internal space S2 may be stored and transported in a state completely isolated from the outside. Through this, the mycobacterium possessed by the sample SP may be prevented from spreading to the outside in the process of moving the sample provided from the patient to the examiner.

In addition, the composition CP, which may be in contact with the human body, may remain sealed in the first internal space S1. Thus, the composition (CP) can not only be free from external contamination, but can also fundamentally block harmful effects on the patient.

In addition, in the process of contacting the sample (SP) and the composition (CP) used for detecting the mycobacterium, the sample (SP) and the second SP (S2) in which the sample SP is stored without opening separately The composition (CP) can be contacted. That is, the sample SP provided from the patient and sealed once in the second internal space S2 may remain completely blocked from the outside air until the infectiousness is removed by the composition CP. Applying pressure to the pressing portion 120 of the sample container 1000 to remove the infectivity of the mycobacterium of the sample SP is performed immediately after the sample is provided by the patient, or in the process of transferring the sample, Or by an inspector detecting mycobacterium.

On the other hand, the specimen (SP) having mycobacterium from which the infectivity has been removed may be smeared and stained on a slide to perform microscopic examination. The dyeing may be performed through acidic dyeing such as Ziehl-Neelsen method or fluorescent dyeing method. In particular, the composition used for detecting mycobacterium according to this embodiment may selectively kill mycobacterium and not kill other bacteria in the sample. For example, the composition used for detection of mycobacterium according to the present embodiment removes the infectivity of mycobacterium, while maintaining the mycobacterium-specific shape with the naked eye, and removes cell walls of other bacteria in the sample. May not be destroyed, but the present invention is not limited thereto. This enables control staining for other bacteria except Mycobacterium, and enables clear visual identification through a microscope.

Although described above with reference to the embodiments of the present invention, which is merely an example and not limiting the present invention, those skilled in the art to which the present invention pertains without departing from the essential characteristics of the embodiments of the present invention. It will be appreciated that various modifications and applications are not possible. For example, each component specifically shown in the embodiment of the present invention may be modified. And differences relating to such modifications and applications will have to be construed as being included in the scope of the invention defined in the appended claims.

100: cap
200: sample receiving unit
300: slit portion
1000: sample container

Claims (7)

delete delete delete delete delete A cap part having a first inner space in which the mycobacterium bactericidal composition is accommodated;
A sample accommodating part screwed with the cap to form a second internal space, wherein the second internal space is separated from the first internal space; And
Including a slit configured to be fitted with the cap portion,
The slit portion,
A first plate that is annular at a planar viewpoint and has a plurality of slits,
A second plate located inside the first plate at a planar view point, the second plate having a plurality of slits and forming a different level from the first plate, and
A plurality of partition walls disposed at a boundary between the first plate and the second plate and protruding toward the cap part;
The sterilizing composition comprises sodium hydroxide, sodium hypochlorite, sodium citrate and a solvent. The method of claim 6,
The cap portion,
Annular fastening body having a coupling means that can be screwed to the sample receiving portion,
Pushing portion disposed on the inner peripheral surface upper side of the annular fastening body,
A side wall portion protruding downward from the edge of the pressing portion;
A cutout portion protruding downward from the center of the pressing portion and inserted into the sidewall portion;
A sealing sheet disposed at a lower end of the side wall part, the sealing sheet forming the first internal space together with the pressing part and the side wall part;
When the pressing portion moves downward,
And the cutout portion projecting from the pressing portion is configured to cut the sealing sheet so that the sterilizing composition contained in the first inner space is moved to the second inner space side.

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