KR101988588B1 - Artificial skin for makeup and preparation method thereof - Google Patents

Abstract

More particularly, the present invention relates to an artificial skin for dressing and a method for manufacturing the artificial skin for a makeup, which is an environmentally friendly and easy to attach and detach without attaching to the skin It can be applied as a dressing material.

Description

Technical Field [0001] The present invention relates to an artificial skin for makeup,

The present invention relates to artificial skin for makeup, and more particularly, to an artificial skin for makeup by applying an adhesive layer to a non-toxic artificial skin to produce an artificial skin for dressing, which is environmentally friendly, The present invention relates to a technique for application as a dressing material.

It is an essential element to make stage performances such as plays and musicals, and to make films such as dramas and movies. However, dressing materials may contain harmful and irritating ingredients to the human body, which may have an adverse effect on the health of actors who are dressed repeatedly over a long period of time. In addition, pain is accompanied by attachment or detachment of a special makeup such as a whisker used in a quasi-draining makeup. In addition, since the adhesive or solvent used is toxic, it is difficult for a person having sensitive skin to make a skin trouble and make a dress.

It has been developed as polyurethane material or silicone material for artificial skin for medical use, which is less toxic, and silicone materials are used for special dressing materials. However, the development of dressing materials which are non-toxic yet easy to attach and detach is reported .

Korean Patent Registration No. 10-1215311 discloses a method for producing artificial whiskers in the form of powders and artificial whiskers produced therefrom. However, when the artificial whiskers adhered to the human skin are desorbed, a great stimulus is given to the skin, It shows the problem of forming edema.

Accordingly, the inventors of the present invention have focused on the fact that artificial skin for a lotion which is coated with an adhesive layer on a non-toxic artificial skin can be produced and applied as a dressing material which is eco-friendly and easy to attach and detach without being stimulated to the skin, I have come to completion.

Patent Document 1. Korean Patent Registration No. 10-1215311

SUMMARY OF THE INVENTION The present invention has been made in view of the above problems, and it is a first object of the present invention to provide artificial skin for makeup which is non-toxic and easy to attach and detach without being stimulated to the skin.

A second object of the present invention is to provide a method for manufacturing artificial skin for dressing according to the present invention.

An aspect of the present invention relates to a thermoplastic elastomer composition comprising (A) an elastomeric film; And (B) an adhesive layer applied on the upper surface of the elastomeric membrane; Wherein the elastomeric membrane comprises 62.3 to 67.3 weight percent vinyl terminated poly (dimethyl) siloxane (CAS No. 68083-19-2), 10 to 15 weight percent acetylated lanolin, And 22 to 23% by weight of silicon dioxide; And 62.3 to 67.3 wt% of hydride terminated poly (dimethyl siloxane) (CAS No.:70900-21-9), 10 to 15 wt% of acetylatedonoline, and 22 to 23 wt% of silicon dioxide A first component comprising a curing agent containing weight%; And 70 to 75.1% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of hydroxyethylcellulose and 14 to 15% by weight of silicon dioxide ; And 70-175.1 wt% of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10-15 wt% of hydroxyethyl cellulose, and 14-15 wt% of silicon dioxide ≪ / RTI > The weight ratio of the subject and the curing agent of the first component is 1: 0.5-1; The weight ratio of the subject and the curing agent of the second component is 1: 0.5-1; Wherein the weight ratio of the first component to the second component is 1: 2-3; Said adhesive layer comprising: 90 to 99% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2) and 0.1 to 1.5% by weight of dipotassium glycyrrhizate; And 70 to 80% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 20% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9) and 1 to 10% by weight of methylhydrosiloxane, dimethylsiloxane copolymer, methylhydrosiloxane (dimethylsiloxane co-polymer, trimethylsiloxane terminated) (CAS No.: 68037-59-2); The weight ratio of the subject of the adhesive layer and the curing agent is 1: 0.5-1; Wherein the elastomer film and the adhesive layer have a thickness ratio of 1: 0.2-0.7.

Another aspect of the present invention is a composition comprising (a) 62.3 to 67.3% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of acetylatedonoline and 22 to 23% ≪ / RTI > And a curing agent containing 62.3 to 67.3% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10 to 15% by weight of acetylatedisonine and 22 to 23% by weight of silicon dioxide, A first component comprising at a weight ratio of 0.5-1; And 70.1 to 75.1% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of acetylatedonoline and 14 to 15% by weight of silicon dioxide; And a curing agent containing 70.1 to 75.1 wt% of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10 to 15 wt% of acetylatedonoline, 14 to 15 wt% of silicon dioxide, Mixing the first component and the second component at a weight ratio of 1: 2 to 1: 2, and coating the resultant mixture at room temperature for 2 to 5 hours to prepare an elastomeric film; And (b) from 90 to 99% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2) and from 0.1 to 1.5% by weight of dipotassium glycyrrhizate; And 70 to 80% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 20% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9) and A pressure-sensitive adhesive mixture obtained by mixing a curing agent containing 1 to 10% by weight of methylhydroxylsiloxane, dimethylsiloxane copolymer and trimethylsiloxane terminate (CAS NO .: 68037-59-2) at a weight ratio of 1: 0.5-1, Pouring and coating the polymer film on the upper surface thereof, then primary curing at 45 to 60 DEG C for 40 to 80 seconds, and secondary curing at room temperature for 30 to 50 minutes, .

According to an embodiment of the present invention, the coating of step (a) and step (b) may be bar coating.

According to the present invention, it is possible to provide artificial skin for a makeup which is non-toxic and easy to attach and detach without stimulation to the skin.

In addition, it is possible to provide a method of manufacturing artificial skin for dressing according to the present invention.

FIG. 1 is an image of a manufacturing process of artificial skin for a dressing according to Example 1 of the present invention. (A) an elastomer film, (b) a step of roll coating (bar coating) an adhesive layer on the upper surface of the elastomer film, ) Artificial skin for makeup]
Fig. 2 is an image showing the method of measuring the adhesive force of the artificial skin for dressing prepared from the elastomer film produced in Production Example 1 of the present invention, and Examples 1 to 4 and Comparative Examples 4 to 7, All.
FIG. 3 is an image of adhesion and desorption test for human skin of artificial skin for a makeup prepared according to Examples 1 to 4 of the present invention. FIG.
Fig. 4 is a schematic view showing a process for producing a stock solution of artificial skin exudates prepared from Preparation Example 1 and Examples 1 to 4 of the present invention.
Fig. 5 is an image of Live / Dead staining method of artificial skin prepared from Preparation Example 1 and Examples 1 to 4 of the present invention [(a) Production Example 1, (b) Example 1, (c) 2, (d) Example 3, (e) Example 4, (f) 1 vol.% Ethanol.
Fig. 6 is an image of a skin animal skin irritation test result of artificial skin prepared from Preparation Example 1 and Examples 1 to 4 of the present invention [(a) Production Example 1, (b) Example 1, (c) Example 2, (d) Example 3, (e) Example 4, (f) 1 vol.% Ethanol.
Fig. 7 is an image showing adhesion and desorption test of a net cloth, which is a special pulp material of an elastomer film prepared from Production Example 1 of the present invention. Fig.
Fig. 8 is an image obtained by attaching a mesh hair, which is a special makeup material, to artificial skin for makeup produced in Example 1 of the present invention.

In the following, various aspects and various embodiments of the present invention will be described in more detail.

An aspect of the present invention relates to a thermoplastic elastomer composition comprising (A) an elastomeric film; And (B) an adhesive layer applied on the upper surface of the elastomeric membrane; Wherein the elastomeric membrane comprises 62.3 to 67.3 weight percent vinyl terminated poly (dimethyl) siloxane (CAS No. 68083-19-2), 10 to 15 weight percent acetylated lanolin, And 22 to 23% by weight of silicon dioxide; And 62.3 to 67.3 wt% of hydride terminated poly (dimethyl siloxane) (CAS No.:70900-21-9), 10 to 15 wt% of acetylatedonoline, and 22 to 23 wt% of silicon dioxide A first component comprising a curing agent containing weight%; And 70 to 75.1% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of hydroxyethylcellulose and 14 to 15% by weight of silicon dioxide ; And 70-175.1 wt% of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10-15 wt% of hydroxyethyl cellulose, and 14-15 wt% of silicon dioxide ≪ / RTI > The weight ratio of the subject and the curing agent of the first component is 1: 0.5-1; The weight ratio of the subject and the curing agent of the second component is 1: 0.5-1; Wherein the weight ratio of the first component to the second component is 1: 2-3; Said adhesive layer comprising: 90 to 99% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2) and 0.1 to 1.5% by weight of dipotassium glycyrrhizate; And 70 to 80% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 20% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9) and 1 to 10% by weight of methylhydrosiloxane, dimethylsiloxane copolymer, methylhydrosiloxane (dimethylsiloxane co-polymer, trimethylsiloxane terminated) (CAS No.: 68037-59-2); The weight ratio of the subject of the adhesive layer and the curing agent is 1: 0.5-1; Wherein the elastomer film and the adhesive layer have a thickness ratio of 1: 0.2-0.7.

The artificial skin for dressing according to the present invention is advantageous in that an adhesive layer is added on a non-toxic elastomer to easily adhere to and detach from a human body.

In particular, although not explicitly described in the following examples or comparative examples, an elastomer is obtained by varying the weight ratio of the first component and the second component, and the ratio of the thickness of the obtained elastomer and the pressure- The storage stability (viscosity change) and durability (heat resistance, moisture resistance) were measured by making artificial skin.

As a result, unlike the other numerical ranges, the storage stability (viscosity change) and durability (heat resistance, moisture resistance, tear strength, and impact strength) , It was confirmed that it can be used for multi-use rather than single-use.

However, when one of the following conditions is not satisfied, the rate of change of viscosity is very large, and durability such as bubble or peeling is remarkably deteriorated.

(i) the weight ratio of the first component and the second component is 1: 2-3, (ii) the thickness ratio of the elastomeric film and the adhesive layer is 1: 0.2-0.7.

Another aspect of the present invention relates to a method of making artificial skin for makeup comprising the following steps.

(a) a subject containing 62.3 to 67.3% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of acetylatedonoline and 22 to 23% by weight of silicon dioxide; And a curing agent containing 62.3 to 67.3% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10 to 15% by weight of acetylatedisonine and 22 to 23% by weight of silicon dioxide, A first component comprising at a weight ratio of 0.5-1; And 70.1 to 75.1% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of acetylatedonoline and 14 to 15% by weight of silicon dioxide; And a curing agent containing 70.1 to 75.1 wt% of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10 to 15 wt% of acetylatedonoline, 14 to 15 wt% of silicon dioxide, Mixing the first and second components at a weight ratio of 1: 2 to 1: 2, and coating the resultant mixture at room temperature for 2 to 5 hours to prepare an elastomer film; And (b) from 90 to 99% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2) and from 0.1 to 1.5% by weight of dipotassium glycyrrhizate; And 70 to 80% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 20% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9) and A pressure-sensitive adhesive mixture obtained by mixing a curing agent containing 1 to 10% by weight of methylhydroxylsiloxane, dimethylsiloxane copolymer and trimethylsiloxane terminate (CAS NO .: 68037-59-2) at a weight ratio of 1: 0.5-1, Pouring and coating on top of the polymer film, primary curing at 45 to 60 ° C for 40 to 80 seconds, and secondary curing at room temperature for 30 to 50 minutes.

The coating of step (a) and step (b) may be carried out by a method such as bar coating, spin coating, brush coating, spray coating, dip coating, Die coating, roll-to-roll coating, and the like, but is not limited thereto.

In particular, when the coating method except for the bar coating is applied, the adhesive layer becomes very thin and it is difficult to have a uniform thickness, so that the bar coating method can be preferably performed.

Also, it has been confirmed that the dipotassium glycyrrhizate contained in the subject of the pressure-sensitive adhesive mixture plays a role of imparting flexibility by improving the tensile strength and elongation of the artificial skin for dressing according to the present invention.

In addition, it was confirmed that when either of the primary and secondary curing was not performed, residues were generated, film thickness retentivity was decreased, or film curling occurred.

Particularly, when the primary curing temperature is lower than 45 ° C or higher than 60 ° C, it is confirmed that the peeling strength is rapidly lowered.

Hereinafter, production examples and embodiments according to the present invention will be described in detail with reference to the accompanying drawings.

Production Example 1: Preparation of elastomer film

A subject containing 65% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 13% by weight of acetylatedisonine and 22% by weight of silicon dioxide; And 65 weight percent of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 13 weight percent of acetylatedisonine and 22 weight percent of silicon dioxide in a weight ratio of 1: 1 A first component; And 73% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 13% by weight of hydroxyethyl cellulose and 14% by weight of silicon dioxide; And a hardening agent containing 73 wt% of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 13 wt% of hydroxyethyl cellulose and 14 wt% of silicon dioxide in a weight ratio of 1: 1 Was mixed at a weight ratio of 1: 2, and bubbles generated in the mixing process were removed using a desiccator and a vacuum pump. The mixture of the mucilous state in which the bubbles were removed was poured onto the polypropylene film on the glass plate, and roll coated (bar coated) using a support rod of 1 mm thickness and a cylindrical bar and then cured at room temperature for 2 hours. The cured elastomer was cut to a certain size to prepare an elastomer film having a thickness of 0.7 mm.

Example 1: Fabrication of artificial skin for make-up

A subject containing 98.7% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2) and 1.3% by weight of dipotassium glycyrrhizate; And 79% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 12% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9) and methyl hydrosiloxane, A curing agent containing 9% by weight of dimethylsiloxane copolymer and trimethylsiloxane terminate (CAS NO .: 68037-59-2) was mixed in a weight ratio of 1: 1, and the bubbles generated in the mixing process were mixed in a desiccator, And a vacuum pump. The pressure sensitive adhesive mixture in the mucilage state from which the air bubbles had been removed was poured on the elastomeric film of Preparation Example 1 and roll coated (bar coated) using a support rod of 1.5 mm in thickness and a cylindrical bar and placed on a 60 ° C electric heater for 1 minute After the primary curing, secondary curing was performed at room temperature for 30 minutes to prepare artificial skin for dressing, in which an adhesive layer was formed on the upper surface of the elastomer film.

Examples 2 to 4

And the primary curing was carried out at 45 ° C, 50 ° C, or 55 ° C in the same manner as in Example 1, and artificial skin for dressing was prepared to give Example 2, Example 3 or Example 4, respectively.

Comparative Example 1

Using the same procedure as in Example 1 except that 100 wt% of vinyl-terminated polydimethylsiloxane (CAS No.: 68083-19-2) except dipotassium glycyrrhizate was used, .

Comparative Example 2

The procedure of Example 1 was followed except that only primary hardening was carried out to prepare artificial skin for dressing.

Comparative Example 3

The procedure of Example 1 was followed except that only secondary hardening was carried out to prepare artificial skin for dressing.

Comparative Examples 4 to 7

Comparative Example 4, Comparative Example 5, Comparative Example 6, or Comparative Example 6 were prepared in the same manner as in Example 1 except that the primary curing was performed at 35 ° C, 40 ° C, 65 ° C, or 70 ° C, 7.

Table 1 below shows the results of confirming residuals, film thickness retention, and curling of artificial skin for a makeup manufactured from Example 1 and Comparative Examples 2 and 3.

Referring to Table 2, in Example 1 in which primary curing was performed at 60 ° C for 1 minute and then secondary curing was performed at room temperature for 30 minutes, no residue was present, the film thickness remained uniform, It can be confirmed that it has not occurred. On the other hand, in Comparative Example 2 in which only the primary curing was performed at 60 占 폚 for 1 minute, there was no residue but the film thickness was uneven and the curling of the film occurred. Further, in Comparative Example 3 in which only the secondary curing was performed at room temperature for 30 minutes, residues were generated, the thickness of the film was uneven, and the membrane was pushed, and it was confirmed that it was not suitable as artificial skin for dressing.

Therefore, by performing both primary and secondary curing, it is possible to manufacture artificial skin for a makeup which is easy to use as a dressing material.

Residue Film thickness retention Curl Example 1 Good Good Good Comparative Example 2 Good Bad Bad Comparative Example 3 Bad Bad Bad

Table 2 shows the results of measurement of the adhesive strength of the above Production Example 1, Examples 1 to 4 and Comparative Examples 4 to 7 with respect to the thickness and the substrate.

As shown in Fig. 2, the artificial skin for dressing was pressed on the substrate, and the load applied while being desorbed was measured. The measurement results were obtained by measuring the peel strength when the film was separated from the substrate by using the load (gf) and the width (mm) of the artificial skin.

As shown in Table 2, in the case of Production Example 1 in which no adhesive layer was formed, quantitative measurement was impossible because the peel strength was lower than the measurement limit range of the measuring apparatus. In Examples 1 to 4 and Comparative Examples 4 to 7 in which the adhesive layer was formed The peel strength is measured and it is confirmed that the adhesion is good.

It can also be seen that the peel strength is drastically lowered in the case of Comparative Examples 4 to 5 in which the first heat treatment at less than 45 ° C is performed and in Comparative Examples 6 to 7 in which the first heat treatment is performed at more than 60 ° C.

Therefore, it has been confirmed that the first heat treatment is preferably performed at a temperature of 45 to 60 캜.

Thickness (mm) Peel strength (gf / mm) Production Example 1 0.7 ± 0.1 Not measurable Example 1 1.1 ± 0.1 1.3 ± 0.04 Example 2 1.1 ± 0.1 1.4 ± 0.04 Example 3 1.1 ± 0.1 1.3 ± 0.08 Example 4 1.1 ± 0.1 1.3 ± 0.08 Comparative Example 4 1.1 ± 0.1 0.2 ± 0.08 Comparative Example 5 1.1 ± 0.1 0.3 ± 0.04 Comparative Example 6 1.1 ± 0.1 0.2 ± 0.08 Comparative Example 7 1.1 ± 0.1 0.2 ± 0.04

Table 3 shows the thicknesses of the above Preparation Example 1, Examples 1 to 4, and Comparative Examples 4 to 7 and the measurement results of adhesion to small animal skin.

As shown in Fig. 2, the skin-adhered skin was surface-treated on one side of the artificial skin for a dressing in the form of a film, pressed on the skin of the small animal squeezed on the substrate, and then peeled and measured for detachment strength. The measurement results were obtained by measuring the peel strength when the artificial skin for a dressing surface treated with a skin adherent substance was separated from a substrate and a small animal skin, using the load (gf) and the width (mm) of the artificial skin.

As shown in Table 3, in the case of Production Example 1 in which the adhesive layer was not formed, quantitative measurement was impossible because the peel strength was lower than the measurement limit range of the measuring device. In Examples 1 to 4 and Comparative Examples 4 to 7 in which the adhesive layer was formed The peel strength is measured and it is confirmed that the adhesion is good.

It can also be seen that the peel strength is drastically lowered in the case of Comparative Examples 4 to 5 in which the first heat treatment at less than 45 ° C is performed and in Comparative Examples 6 to 7 in which the first heat treatment is performed at more than 60 ° C.

Therefore, it has been confirmed that the first heat treatment is preferably performed at a temperature of 45 to 60 캜.

Thickness (mm) Peel strength from substrate
(gf / mm) Peel strength from skin
(gf / mm) Production Example 1 0.7 ± 0.1 Not measurable Not measurable Example 1 1.1 ± 0.1 0.9 ± 0.20 1.6 ± 0.36 Example 2 1.1 ± 0.1 0.8 ± 0.20 1.6 ± 0.16 Example 3 1.1 ± 0.1 0.9 ± 0.12 1.5 ± 0.08 Example 4 1.1 ± 0.1 0.8 ± 0.04 1.6 ± 0.24 Comparative Example 4 1.1 ± 0.1 0.1 ± 0.04 0.3 ± 0.08 Comparative Example 5 1.1 ± 0.1 1.2 ± 0.08 0.3 ± 0.04 Comparative Example 6 1.1 ± 0.1 0.2 ± 0.04 0.4 ± 0.08 Comparative Example 7 1.1 ± 0.1 0.0 ± 0.04 0.4 ± 0.04

Also, as shown in FIG. 3, it can be confirmed that the artificial skin for dressing of Examples 1 to 4 can be attached and detached to human skin.

Table 4 shows the tensile strengths (JIS K6301, K6911 ASTM 638-84) and elongation ratios (JIS K6301, K6911, ASTM 638-84) of Example 1 and Comparative Example 1.

Referring to Table 4, it can be seen that the tensile strength and the elongation percentage of the synthetic artificial skin of Comparative Example 1 except dipotassium glycyrrhizate were significantly lowered than those of Example 1 in the production of the adhesive layer. As a result, it can be confirmed that the dipotassium glycyrrhizate significantly improves the flexibility of artificial skin for a makeup.

Tensile strength (km / cm ² ) Elongation (%) Example 1 75 210 Comparative Example 1 32 101

Next, the cytotoxicity of artificial skin prepared from Preparation Example 1 and Examples 1 to 4 was evaluated through a dissolution test according to the International Organization for Standardization (ISO 10993) standard. The eluate of artificial skin for the dressing was added to the cells to be cultured for 24 hours, and then the eluate was removed. Then, acridine orange (AO) showing green fluorescence in living cells and propidium Cell viability was measured by Live / Dead staining using iodide (PI).

Fig. 4 is a schematic view showing a process for producing a stock solution of artificial skin exudates prepared from Preparation Example 1 and Examples 1 to 4 of the present invention. The specimens were washed in a 70% aqueous ethanol solution for 30 minutes and sterilized by irradiation with ultraviolet rays for 15 minutes. The elution was carried out using a medium containing 1% antibiotics, which did not contain serum, and eluted at 37 ± 2 ° C for 48 hours in an area / volume ratio of 3 cm ² / mL according to the thickness of the specimen.

Fig. 5 is an image of Live / Dead staining method of artificial skin prepared from Preparation Example 1 and Examples 1 to 4 of the present invention [(a) Production Example 1, (b) Example 1, (c) 2, (d) Example 3, (e) Example 4, (f) 1 vol.% Ethanol. Balb / c 3T3 cells were plated in 96-well plates at a density of 1 × 10 ⁴ cells / well. After culturing for 24 hours, 200 μL of the eluate was added to the wells and cultured for 24 hours. After 24 hours, the eluate was removed and the cell culture medium containing 0.67 μM of AO and 75 μM of PI was added to the wells and kept in a cell culture incubator for 30 minutes in the absence of light After that, the number of viable cells (green) and dead cells (red) were counted through a microscope and cell viability was measured. As a control, serum-free medium containing ethanol at a concentration of 1% was used.

Table 5 shows cell viability.

Referring to Table 5, it can be confirmed that the eluate membrane prepared from Preparation Example 1 and the elution products of artificial skin for dressing prepared from Examples 1 to 4 have almost no toxicity to cells, and the cell survival rate is 95% Respectively.

Survival rate (%) Production Example 1 97.6 ± 1.0 Example 1 98.3 ± 0.5 Example 2 98.7 ± 0.5 Example 3 98.2 ± 1.7 Example 4 98.7 ± 0.4 1 vol% ethanol 30.1 ± 2.0

Next, the skin irritation properties of the artificial skin for dressing prepared in Production Example 1 and Examples 1 to 4 of the present invention were examined.

First, a small animal skin irritation test was conducted on artificial skin for makeup. Skin irritation test by the International Organization for Standardization (ISO 10993) was carried out to evaluate skin irritation of artificial skin for makeup.

The artificial skin of 0.5 cm × 0.5 cm in size was washed with 70% ethanol aqueous solution for 30 minutes, irradiated with ultraviolet rays for 15 minutes and sterilized, and then epilated using a hair clipper. The skin was contacted with a mouse and fixed with a band tape to expose the skin to artificial skin for 24 hours. Twenty-four hours later, the artificial skin was removed and the presence of erythema and oedema was observed, and the grade was judged and added to confirm skin irritation. As a control group, an aqueous solution of 70 vol.% Ethanol was soaked in gauze.

Table 6 below shows skin irritancy ratings due to erythema and edema formation.

reaction Irritability rating Eschar and eschar forms  No erythema 0  Very mild erythema (hard to detect) One  Pronounced erythema 2  Common erythema 3  Severe erythema 4 Oedema type  No edema 0  Very mild edema (hard to detect) One  Pronounced edema 2  Usually this edema 3  Severe edema 4 Maximum possible stimulus rating 8

Fig. 6 shows images (a), (b), and (c) of Example 1 and Example (c) of a skin animal skin irritation test result of artificial skin for make up prepared from Production Example 1 and Examples 1 to 4 of the present invention 2, (d) Example 3, (e) Example 4, (f) 1 vol.% Ethanol]. Table 7 shows the skin irritation grade from FIG. 6 and Table 6.

Referring to Table 7, it can be confirmed that the synthetic artificial skin prepared from Preparation Example 1 and Examples 1 to 4 is less than the first grade, which is much lower than the target value of the irritancy grade for the skin of the small animal, which is the third grade.

Rating Production Example 1 0 ± 0 Example 1 0 ± 0 Example 2 0 ± 0 Example 3 0 ± 0 Example 4 0 ± 0 1 vol% ethanol 2

Secondly, human skin irritation test was performed on artificial skin for makeup.

Ten women of 20 to 29 years old were attached to the facial skin (U zone, T zone) for 3 hours, once per week, respectively, for the dressing skin prepared in Preparation Example 1 and Examples 1 to 4, and then skin irritation Were evaluated on the basis of erythema reaction and itching. As a result of the evaluation, no skin eruption reaction or itching was observed on human skin.

Therefore, it was confirmed that the artificial scalp skin of the present invention had no skin irritation.

The number of viable microorganisms on the surface of the elastomer film of Production Example 1 of the present invention before adhesion to the skin was measured. 1 mL of the bacterial suspension is collected with a micropipette to obtain Pertirilm Aerobic Count Plates for general bacteria, Pertirilm Escherichia coli Plates for E. coli, Pertirilm Yeast and Mold Count Plates ), Pertirilm Staphylococcus aureus Count Plates for Pseudomonas aeruginosa, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Pseudomonas aeruginosa, and Pseudomonas aeruginosa.

Table 8 shows microbial standards of domestic cosmetics (finished products).

division Korea Total number of aerobic live bacteria For children ≪ 500 CFU / g Products used around the eyes ≪ 500 CFU / g Other products ≪ 10 ³ CFU / g Pathogenic microorganism E. coli
P. aeruginosa
S. aureus
No detection at 0.1 g of product

Table 9 below shows the results of measurement of microbial viable count on the surface of the elastomer membrane of Production Example 1 of the present invention before adhesion to the skin based on Table 8 [* aerobic bacteria = general bacteria + yeast / mold].

As shown in Table 9, all the test items were measured below the reference value, and it was confirmed that no microbial contamination was observed when the elastomer film was worn on the face.

* Aerobic bacteria Escherichia coli
(E. coli) Yellow vinegar
(S. aureus) P. aeruginosa
(P. aeruginosa) Production Example 1 Below standard Below standard Below standard Below standard

Meanwhile, FIG. 7 shows an image showing adhesion and desorption test of the special polymer material of the elastomer film prepared from Production Example 1 of the present invention.

One of the special makeup tools used in the historical drama, the mens whiskers (correct name is the whiskers), was used to test whether the special makeup tools were easy to attach and detach onto the elastomer. A net whisker is a mustache that is planted one by one in the same direction as a skill needle embroidered on a thin network of skin color. The net whiskers have a relatively short dressing time and easy connection of the whiskers. It can be used many times, so it is widely used not only in stage such as opera but also in photography.

As a result of the test, it was confirmed that the mesh whisker can be adhered to and detached from the elastomer membrane by using Spirit gum.

Fig. 7 is an image obtained by attaching a mesh hair, which is a special makeup material, to artificial skin for makeup produced in Example 1 of the present invention.

As shown in FIG. 8, the artificial skin for a lotion with a nettle beard is attached to the skin of the human body with a mustache (upper part of the figure) and a beard (lower part of the figure) and is attached and detached several times. As a result, there was.

Therefore, according to the present invention, it is possible to provide an artificial skin for a lotion which is coated with an adhesive layer on a non-toxic artificial skin, which can be applied as a dressing material which is eco-friendly and can be attached and detached easily without stimulation to the skin.

Claims (3)

(A) an elastomer film; And
(B) an artificial skin for a dressing comprising an adhesive layer applied on an upper surface of the elastomer film;
Said elastomeric membrane comprising 62.3 to 67.3% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of acetylatedonoline and 22 to 23% by weight of silicon dioxide; And 62.3 to 67.3% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10 to 15% by weight of acetylatedonoline and 22 to 23% by weight of silicon dioxide A first component; And
A subject containing 70.1 to 75.1% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of hydroxyethyl cellulose and 14 to 15% by weight of silicon dioxide; And 70-175.1 wt% of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10-15 wt% of hydroxyethyl cellulose, and 14-15 wt% of silicon dioxide ≪ / RTI >
The weight ratio of the subject and the curing agent of the first component is 1: 0.5-1;
The weight ratio of the subject and the curing agent of the second component is 1: 0.5-1;
Wherein the weight ratio of the first component to the second component is 1: 2-3;
Wherein the adhesive layer comprises a mixture of 90 to 99% by weight of vinyl terminated polydimethylsiloxane (CAS No. 68083-19-2) and 0.1 to 1.5% by weight of dipotassium glycyrrhizate; And 70 to 80% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 20% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9) and 1 to 10% by weight of methylhydroxylsiloxane, dimethylsiloxane copolymer, trimethylsiloxane terminate (CAS NO .: 68037-59-2);
The weight ratio of the subject of the adhesive layer and the curing agent is 1: 0.5-1;
Wherein the thickness ratio of the elastomer film and the adhesive layer is 1: 0.2-0.7. (a) a subject containing 62.3 to 67.3% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of acetylatedonoline and 22 to 23% by weight of silicon dioxide; And a curing agent containing 62.3 to 67.3% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10 to 15% by weight of acetylatedisonine and 22 to 23% by weight of silicon dioxide, A first component comprising at a weight ratio of 0.5-1; And
A subject containing 70.1 to 75.1% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 15% by weight of acetylatedonoline and 14 to 15% by weight of silicon dioxide; And a curing agent containing 70.1 to 75.1 wt% of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9), 10 to 15 wt% of acetylatedonoline, 14 to 15 wt% of silicon dioxide, Mixing the first component and the second component at a weight ratio of 1: 2 to 1: 2, and coating the resultant mixture at room temperature for 2 to 5 hours to prepare an elastomeric film; And
(b) a subject containing from 90 to 99% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2) and from 0.1 to 1.5% by weight of dipotassium glycyrrhizate; And 70 to 80% by weight of vinyl terminated polydimethylsiloxane (CAS No.: 68083-19-2), 10 to 20% by weight of hydride terminated polydimethylsiloxane (CAS No.:70900-21-9) and A pressure-sensitive adhesive mixture obtained by mixing a curing agent containing 1 to 10% by weight of methylhydroxylsiloxane, dimethylsiloxane copolymer and trimethylsiloxane terminate (CAS NO .: 68037-59-2) at a weight ratio of 1: 0.5-1, Pouring and coating the polymer film on the upper surface thereof, and then primary curing at 45 to 60 DEG C for 40 to 80 seconds, followed by secondary curing at room temperature for 30 to 50 minutes. 3. The method of claim 2,
Wherein the coating of step (a) and step (b) is bar coating.

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