KR101956403B1 - Eye fatigue suppression composition and food and drink containing same - Google Patents

Abstract

The present invention provides an eye fatigue inhibiting composition comprising resveratrol as an active ingredient. The inventors of the present invention have found that resveratrol has an effect of suppressing eye fatigue as a result of repeated research, and completed a composition for inhibiting eye fatigue using the resveratrol of the present invention as an active ingredient. The composition of the present invention is absorbed from the oral cavity, and effectively suppresses eye fatigue.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a composition for suppressing eye fatigue,

The present invention relates to a composition for suppressing eye fatigue and a food and drink containing the same. In addition, the present invention relates to the above composition, which is administered in a form in which it exhibits an immediate effect on eye fatigue and, moreover, is absorbed from the oral cavity, and a food and beverage containing it.

About eye fatigue

According to reports (non-patent documents 1 and 2) of the EMF Expert Committee of the Association of Electronic Information Technology Industries, Inc., eye fatigue (eye fatigue) is explained as follows. Eye fatigue is fatigue that is recovered in a relatively short period of time by taking a certain amount of rest due to general eye fatigue, such as heavy eyes and blurred objects. It develops as a stable fatigue by accumulation of eye fatigue. Stable fatigue refers to a condition in which the fatigue state is remarkably morbid and can not be restored even when resting. By continuing to see, complaints of discomfort, uncomfort, total discomfort, pressure, headache, diminished vision, dizziness, nausea.

Specifically, eye fatigue consists of two types of muscle fatigue in the eye control system, such as the eyebrows that affect the focus control function, and central fatigue in the visual information processing that affects the cognitive function. One of the causes of myopia in the eye control system is to keep a close eye on eyes for a long time. When a close-up object such as a PC display or a book is observed for a long time, a specific muscle of the eye continues to be tense, and muscle fatigue material accumulates and appears as eye fatigue. The achievement of the present invention relates to eye fatigue.

The importance of eye fatigue suppression

In recent years, people who appeal to eye fatigue have been increasingly demanded, and a variety of products, such as a product that gives a warmth or a cold feeling, and a massage device around the eyes, have been developed. The cause of eye fatigue is caused by overtightness or mental strain of eyes such as reading, watching work, observation work, etc. Among them, VDT (Visual Display Terminal) work which has increased sharply with the spread of personal computer The eye fatigue caused by the eye is greatly increased, and a solution thereof is desired. However, there is no way to improve the eye fatigue state in an easy and simple manner even during an operation, and a new improvement method has been demanded.

Compounds with reported eye fatigue action

Astaxanthin (Non-Patent Documents 3 and 4) and Crocetin (Non-Patent Document 5) are examples of compounds that have been reported to have effects on eye fatigue and stable fatigue so far. And an immediate effect can not be expected. Cassis anthocyanins (non-patent documents 6 and 7), which have been reported to be relatively quickly identified, take several hours to show their effects, and the effects are not sufficient.

About Resveratrol

Resveratrol, or 3,5,4'-trihydroxystilbene, is a compound belonging to the stilbene phytorelacin group, which includes various trihydro and tetrahydroxystilbene groups. Resveratrol is contained in grapes, roots, peanuts, etc., and is most abundantly present in red grapes and known as a substance having desirable pharmacological effects. So far, there has been a report on the pharmacological effect of resveratrol. Patent Document 1 discloses resveratrol as an oral composition which is useful for preventing arthralgia of the joints of the extremities, preventing recurrence of back pain and sciatica, and relieving symptoms. Patent Document 2 discloses resveratrol as a pharmaceutical composition which inhibits the circulatory mechanical disorder by peroxisome proliferation-responsive receptor a activation. Patent Document 3 discloses resveratrol as a composition for the prevention and treatment of symptoms related to cold and flu-like type diseases. Patent Document 4 discloses resveratrol as a food composition that improves edema and cold of legs. Patent Document 5 discloses resveratrol as a substance having an AMPK activating action and also discloses that a food or drink containing resveratrol has a lipid metabolism activator, an obesity inhibitor, a diabetes preventive improvement agent, a liver hypertrophy inhibitor, and a fatty liver inhibitory effect. Patent Document 6 discloses a food or the like containing resveratrol as an anti-inflammatory nutritional supplement composition. Patent Document 7 discloses a food or drink containing resveratrol as having a blood glucose lowering effect. Patent Document 8 discloses that resveratrol has an effect of improving endurance. Patent Document 9 discloses resveratrol as a composition for fine skin. Patent Document 10 discloses resveratrol as an adiponectin production promoter.

In terms of eye diseases, a method has been disclosed in which resveratrol activates sirtuin and thereby treats various symptoms of eyes (Patent Documents 11, 12 and 13). However, these documents have taken concrete examples of long-term administration to patients suffering from pathological conditions such as glaucoma or macular degeneration, and effects of direct injection or implantation on the eye, and to solve eye fatigue easily and promptly There is no disclosure or suggestion about anything. Non-patent document 8 shows that resveratrol has antioxidative and hyperproliferative actions in human retinal pigment epithelial cells, and Non-Patent Document 9 discloses that resveratrol has an action of expanding the retinal artery of a pig extracted with resveratrol Report. However, resveratrol can not be expected to have an immediate effect on eye fatigue unless it is injected directly into the eye because resveratrol is virtually absent as resveratrol itself due to absorption by the intestinal tract due to intestinal absorption and by glucuronic acid incorporation .

Japanese Patent Application Laid-Open No. 2001-72582 Japanese Patent Application Laid-Open No. 2003-300904 Japanese Patent Publication No. 2003-505500 Japanese Patent Application Laid-Open No. 2005-052085 Japanese Patent Application Laid-Open No. 2006-273834 Japanese Patent Publication No. 2006-528950 Japanese Patent Application Laid-Open No. 2007-126390 Japanese Patent Application Laid-Open No. 2007-145809 Japanese Patent Application Laid-Open No. 2008-088123 Japanese Patent Application Laid-Open No. 2008-255040 Japanese Patent Application Laid-Open No. 2006-298876 Japanese Patent Publication No. 2008-538215 Japanese Patent Publication No. 2009-529538

 Technical report: JEITA-EMF-R0504 Investigation report including use of the term "stable fatigue"  Human Interface Symposium, RONBUN No.2332, 2006  Clinical Ophthalmology 58 (6), 1051-1054, 2004  Clinical Medicine 21 (4), 431-436, 2005  Visual Science 28 (2), 77-84, 2007  New Ophthalmology 23 (1), 129-133, 2006  New Medicine and Clinics 56 (2), 180-188, 2007  Chemico-biological interactions, Jan 15, 151 (2), 143-149, 2005  Investigative ophthalmology & visual science, Sep 48 (9), 4232-4239, 2007

It is an object of the present invention to provide a composition for suppressing eye fatigue caused by VDT work or the like.

The present invention provides an eye fatigue inhibiting composition comprising resveratrol as an active ingredient.

The inventors of the present invention have found that resveratrol has an effect of suppressing eye fatigue as a result of repeated research, and completed a composition for inhibiting eye fatigue using the resveratrol of the present invention as an active ingredient. The composition of the present invention absorbs from the oral cavity, and effectively suppresses eye fatigue.

The composition of the present invention has a surprising immediate effect of suppressing eye fatigue by only taking a single time in 10 minutes or less. In addition, the composition of the present invention has a remarkable effect of predominating compared with 50 mg of cassis anthocyanin at an intake of 1 to 10 mg. More surprisingly, the composition of the present invention is absorbed from the oral mucosa, and is effective only by staying in the oral cavity.

In addition, resveratrol is a substance contained in grape, hoof roots, and peanut, and is high in safety and has good consumer image.

Fig. 1 shows the result of the near-point measurement in the test for confirming the effect of resveratrol on a subject-by-subject basis.
FIG. 2 is a graph summarizing the results of the measurement of the near point system in the test for confirming the effect of resveratrol.
Fig. 3 shows the result of the near-point measurement in the test for confirming the concentration dependence of the effect of resveratrol on a subject-by-subject basis.
FIG. 4 is a graph summarizing the results of the near point measurement in the test for confirming the concentration dependence of the effect of resveratrol.
5 is a graph showing the results of a test to confirm that resveratrol is absorbed in the oral cavity.

The present invention provides an eye fatigue inhibiting composition comprising resveratrol as an active ingredient. Resveratrol is a compound represented by the following structural formula.

[Chemical Formula 1]

The dose per dose is preferably 1 to 10 mg, more preferably 1 to 6 mg.

In addition, the present invention provides the eye fatigue inhibiting composition characterized by exhibiting an effect on eye fatigue immediately. The term " effective " refers to the effect within 5 minutes to 1 hour from administration, and more preferably within 10 minutes to 30 minutes from administration.

In addition, the present invention provides the eye fatigue inhibiting composition, which is administered in a form absorbed from the oral cavity. The form absorbed from the oral cavity refers to a form in which the composition is taken from the oral cavity. Specifically, when the solution containing the composition of the present invention is stirred for 1 minute to 10 minutes in the oral cavity, 10 to 30% . However, since the composition of the present invention is safe for a living body, it may be swallowed later, and it does not inhibit the absorption from the organs other than digestive organs or the like other than oral cavity.

Further, the present invention provides a food and drink containing the eye fatigue inhibiting composition. There is no limitation to food and drink, for example, foods such as soft drinks, confectionery, ice cream, dairy products, alcoholic beverages and meat. Considering that the composition of the present invention is absorbed from the oral cavity, it is particularly preferable to use chewing gum, candy, or jelly, which has a long time to stay in the oral cavity. It is also preferable that the food product is prepared so that an intake amount of resveratrol per one time is 1 to 10 mg, more preferably 1 to 6 mg.

Hereinafter, embodiments of the present invention will be described in more detail with reference to examples.

Example 1

Measuring the effect of resveratrol on eye fatigue

<Test Method>

Resveratrol used VINEATROL (R) WD from Sun Bright. This product contains 0.30% resveratrol in the glycerin preparation (analysis value). For each dose, 2 g of the glycerin preparation (containing 6 mg as resveratrol) was added to 10 ml of distilled water.

Cassis anthocyanin used "Cassis extract 35" (powder) manufactured by Tama Biochemical Co., Ltd. This powder contains 35.0% or more of the total of four kinds of delphinidin-3-rutinoside, cyanidin-3-rutinoside, delphinidin-3-glucoside and cyanidin-3-glucoside in the powder value). For taking one dose per day, 142.9 m of the powder, which was adjusted to 10 ml with distilled water, was used so that the total of the four kinds was 35.0% and 50 mg as an anthocyanin was contained.

Subjects were prepared for 2 out of 3 subjects for a control period of 18 days, a resveratrol intake period of 9 days, a washout period of 14 days, and a cassis anthocyanin intake period of 9 days. For one subject, a control period of 5 days, a resveratrol intake period of 5 days, a washout period of 5 days, and a cassis anthocyanin intake period of 5 days were prepared. During the test period, the following measurements were performed every evening.

First, as a preload measurement, a questionnaire about the near point measurement, eye fatigue, and body fatigue was performed. Next, a load of 20 minutes was applied. That is, a -2 to -3 diopter lens was worn and a PC operation (document input) was performed for 20 minutes in the bright room. During this period, resveratrol solution was used for the resveratrol intake period, cage anthocyanin solution was used for the cassis anthocyanin intake period, and the mixture was stirred in the oral cavity (vaginal absorption) using the tongue or cheek , Respectively. After that, the input operation was continued for another 10 minutes. Immediately after the end of the load test, a questionnaire about the near-point measurement, eye fatigue, and body fatigue was performed as a post-load measurement.

Measurements of the myofibers were made using D'ACOMO (Work Co., Ltd.), a binocular open-type positive refraction near-point system, according to the instruction manual. Specifically, the indicator is slowly approached to the subject to be examined (in the superiority) and the subject is instructed to continue to specify, and the position of the indicator when the focus begins to blur is read. When this is assumed to be a cm, 100 / a is the refraction value of the near point (the unit is Diopter; D), and the analysis is carried out using this measurement value. In general, it is said that when the eye becomes fatigued, the closest distance (the nearest adjustment distance) in which the object is clearly visible from the lowering of the focus control is gradually getting distant, and the eye fatigue can be measured based on this value. In addition, the near point measurement was performed five times in one measurement, and the average value was used for analysis.

On the other hand, concerning questionnaire about eye fatigue, body fatigue, item about eye fatigue tears eyes, eyes are sick, eyes become sick, tears come out, eyes become sick, eyes dry, eyelids heavily For 7 items of feeling, we do not feel at all about four items of head and shoulders are stiff, head is heavy, head is heavy in item about physical fatigue, shoulder is stiff, feels slightly, feels slightly, feels slightly strong, feels very strongly The evaluation was carried out in five steps. The score was scored as 0 for no feeling and 4 for very strong feeling.

The interpretation of these experimental results was carried out by performing a multiple test (Tukey) of the mean population for the comparison between the groups and setting the significance level to 0.05. The paired t test was used to test the test results before and after the load test, and the test was conducted at a significance level of 0.05.

<Test Results>

The control period of 2 subjects was 18 times, the reseratrol and cassis anthocyanin intake period was 9 times, and the results of 5 measurements of each period of one subject were as shown in Table 1 below. The graph is shown in Fig.

Table 2 summarizes the results of the near-point measurement of three subjects. This is shown in FIG. 2 as a graph.

Compared with the control group, the resveratrol group showed significant improvement in the lowered refractive index by the addition of the load. Cassis anthocyanin intake group was significantly improved, but the improvement effect of resveratrol was significantly higher.

On the other hand, the results of the questionnaire regarding eye fatigue and body fatigue were as shown in Table 3.

When the analysis of three subjects was performed for the amount of change obtained by subtracting the post-load value from before the load, the result was as shown in Table 4.

In the control group, each item was negative value, and deterioration was confirmed. In the resveratrol group, 4 items of eye fatigue, dry eyes, heavy eyelids, and heavy head were significantly improved. In the group of cassis anthocyanin ingestion, the eyes were dry. In comparison between the resveratrol group and the cassis anthocyanin intake group, significant differences were found in feeling eyelids heavily, and it became clear that resveratrol was high in body sensibility.

[Example 2]

Determination of the concentration dependence of the effect of resveratrol on eye fatigue

<Test Method>

For the two subjects, a control period of 5 days, a 3 mg resveratrol intake period of 5 days, and a 1 mg resveratrol intake period of 5 days were prepared. During this test period, the same measurements as in Example 1 were performed every evening. However, unlike Example 1, water was vortically absorbed during the control period.

Resveratrol was prepared by taking 1 g of glycerin (containing 3 mg as resveratrol) or 0.33 g (1 mg as resveratrol) in 10 ml of distilled water for each dose.

<Test Results>

As a result of comparing the effects of the resveratrol with 1 or 3 mg of resveratrol on the fatigued eyes compared with the case of varenicidal absorption of water as a control, This is shown in Fig. 3 as a graph.

The results of the two subjects are summarized in Table 6. This is shown in FIG. 4 as a graph.

The intake of 1, 3 mg resveratrol showed a tendency to improve the refraction value in a dose-dependent manner, and each of them was significantly improved in control.

On the other hand, the results of the questionnaire regarding eye fatigue and body fatigue were as shown in Table 7.

In addition, two subjects were analyzed for the amount of change obtained by subtracting the post-load value from the load value, and the results shown in Table 8 were obtained.

In the control group, the eye became tired, the eyelids felt heavy, and the head was heavy. On the other hand, the intake of 1 mg of resveratrol, eye fatigue, heavy head is significantly improved two items. Although no dose-dependent improvement trends were observed, improvement trends in both groups were confirmed as compared with control, and it became clear that eye fatigue and body fatigue were improved by ingesting 1 to 3 mg.

Example 3

Confirmation of oral absorbability of resveratrol by vocal absorption test

To confirm that resveratrol was absorbed from the oral cavity, when the resveratrol solution was stirred for a certain period of time so as not to swallow the tongue or cheek in the oral cavity, a vocal absorption test was conducted to investigate the residual rate of resveratrol in the discharged liquid.

<Test Method>

The requisite amount of resveratrol preparation was weighed to give an intake of resveratrol of 1 or 2 mg per dose, 5 mM phosphoric acid buffer (pH 6.5) warmed to 37 DEG C was added, and the solution was adjusted to 10 mL to be a test solution . Subjects (2) were rinsed with distilled water and stabilized for 5 minutes. Thereafter, 10 ml of the above test solution was put into the mouth, and the cheek or tongue was used with stirring at a time of 1 time / 1 second so as not to swallow for a predetermined time. The solution in the buccal cavity was dispensed as completely as possible into a 50 ml Falcon tube and immediately rinsed 3 times with 5 mM phosphate buffer (pH 6.5) (using 20 ml Buffer in total 3 times). The amount of resveratrol remaining after the discharged liquid was added was measured. Specifically, the discharged solutions were combined and adjusted to 50 ml with 5 mM phosphate buffer (pH 6.5), and the same amount of acetonitrile was added. After centrifugation at 3000 rpm, 10 minutes, and 4 ° C, the supernatant was adjusted to 0.45 μm Filtered, and subjected to HPLC analysis.

<Test Results>

As a result of analyzing the results of the triplicate test, the results shown in Table 9 were obtained. This is shown in Fig. 5 as a graph.

From these test results, it was found that the absorption amount of resveratrol in the oral cavity was concentration-dependent, and the absorption rate of 20 to 25% was expected in the stirring test of 1 to 2 mg / 10 ml for 5 minutes.

Hereinafter, the present invention will be described in more detail with reference to examples, but the scope of the present invention is not limited thereto.

Using preparations containing about 8% resveratrol, chewing gum, candies, and confectioneries were prepared in a conventional manner. The prescription was shown below.

The amount of intake of each food and drink was adjusted to 1 to 10 mg of resveratrol by using one (3 g) of chewing gum, one (5.4 g) of candy, and four (4) of tablets as the ingestion amount.

Example 4

Chewing gum was prepared according to the following prescription.

Gum base 20.0%

Sugar 54.7

Glucose 15.0

Syrup 9.3

Fragrance 0.5

Resveratrol  Containing formulation 0.5

                                  100.0%

Example 5

Chewing gum was prepared according to the following prescription.

Gum base 20.0%

Sugar 54.0

Glucose 10.0

Starch syrup 13.0

Fragrance 0.5

Resveratrol  Containing formulation 2.5

                                  100.0%

Example 6

Chewing gum was prepared according to the following prescription.

Gum base 20.0%

Sugar 50.5

Glucose 14.0

Starch syrup 13.0

Fragrance 0.3

Resveratrol  Containing formulation 1.2

                                  100.0%

Example 7

Candies were prepared according to the following prescription.

Sugar 50.0%

Starch syrup 33.0

Citric Acid 1.0

Fragrance 0.2

L-menthol 1.0

Resveratrol formulation 0.3

Water 14.5

                                  100.0%

Example 8

Candies were prepared according to the following prescription.

Sugar 38.0%

Starch syrup 41.0

Citric Acid 1.0

Fragrance 0.2

Resveratrol formulation 1.5

Water 18.3

                                  100.0%

Example 9

The preparation was prepared according to the following prescription.

Sugar 76.3%

Glucose 19.0

Sucrose fatty acid ester 0.2

Fragrance 0.2

Resveratrol formulation 0.3

Water 4.0

                                  100.0%

Example 10

The preparation was prepared according to the following prescription.

Sugar 74.7%

Lactose 18.9

Sucrose fatty acid ester 0.15

Resveratrol formulation 2.0

Water 4.25

                                  100.0%

Example 11

The preparation was prepared according to the following prescription.

Sugar 73.7%

Lactose 18.9

Sucrose fatty acid ester 0.15

Resveratrol formulation 3.12

Water 4.13

                                  100.0%

This application is based upon and claims the benefit of priority from Japanese Patent Application No. 2011-144389 filed on June 29, 2011, the contents of which are incorporated herein by reference.

Claims (12)

An eye fatigue inhibiting composition comprising resveratrol as an active ingredient. The method according to claim 1,
Wherein the composition exhibits an effect on eye fatigue within 5 minutes to 1 hour after administration. The method according to claim 1,
Wherein the composition is administered in a form absorbed from the oral cavity. 3. The method of claim 2,
Wherein the composition is administered in a form absorbed from the oral cavity. A food composition for suppressing eye fatigue, which contains the eye fatigue inhibiting composition according to claim 1. An eye fatigue-suppressing food composition comprising the eye fatigue-preventing composition according to claim 2. An eye fatigue suppressing food composition comprising the eye fatigue inhibiting composition according to claim 3. An eye fatigue-suppressing food composition comprising the eye fatigue-reducing composition according to claim 4. 6. The method of claim 5,
Wherein an intake amount of resveratrol per one time is 1 to 10 milligrams. The method according to claim 6,
Wherein an intake amount of resveratrol per one time is 1 to 10 milligrams. 8. The method of claim 7,
Wherein an intake amount of resveratrol per one time is 1 to 10 milligrams. 9. The method of claim 8,
Wherein an intake amount of resveratrol per one time is 1 to 10 milligrams.

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