KR101942255B1 - An apparatus and a method for genotyping - Google Patents

Abstract

An embodiment of the present invention is an apparatus for automatically feeding a plurality of solutions to a lab-on-a-chip sequentially, performing an electrochemical reading on a solution in which PCR and hybridization have been performed while passing through a lab-on-a- To provide a method for identifying a gene using the gene. A gene discrimination apparatus according to an embodiment of the present invention includes: a lab-on-a-chip in which a plurality of plate materials having different shapes are laminated and a plurality of kinds of solutions are introduced, mixed, and discharged; A tray for fixing the lab-on-a-chip to the upper surface, and a gene reading module for performing electrochemical reading of the gene introduced into the lab-on-a-chip. A solution supply module located in a direction of a lower surface of the tray and having a plurality of scans, and operating each of the plurality of scans to supply a solution to the lab-on-a-chip; A hybridization module for generating a readout solution to be read and delivering the readout solution to an operation mechanism module so that a readout solution is provided to the lab-on-a-chip; And a solution is supplied to the lab-on-a-chip, and a solution stored in each of the plurality of scans is supplied to the lab-on-a-chip to perform an electrochemical reading on the gene in the reading solution. And a control unit for performing control on the control unit.

Description

TECHNICAL FIELD [0001] The present invention relates to a gene discrimination apparatus,

The present invention relates to a gene discrimination apparatus and a gene discrimination method using the same, and more particularly, to a method and apparatus for automatically supplying a plurality of solutions to a lab-on-a-chip, The present invention relates to a device for performing electrochemical reading and transmitting results, and a method for identifying genes using the same.

Lap-on-a-chip (Lap-on-a-chip) is a type of biochip that allows researchers to conduct research in a laboratory with a small chip. A chip made of plastic, glass, or silicon to make microchannels of nanometer (nm) or less, which enables rapid substitution of experiments or research processes that can be done in existing laboratories with only very small samples or samples. to be.

Since the process such as pretreatment, reaction, separation, and detection of biomaterials or samples can be performed continuously on a single chip, it is excellent in terms of speed, price, and efficiency. The lab-on-a-chip technology is used in a variety of fields such as drug discovery in the pharmaceutical industry, medical diagnostic equipment, health screening equipment, chemical or biological process monitoring, portable environmental pollutant analysis equipment and unmanned chemical / biological agent detection / Can be applied.

In recent years, there has been an increase in the use of such lab-on-a-chip to analyze genes for marine fish species and to provide information by identifying the origin of a living organism. Accordingly, , And the demand for a miniaturized gene discrimination device for the convenience of movement for the efficiency of gene reading is increasing.

Korean Patent No. 10-1302353 entitled "Genetic Quantitative Detection Real-Time PCR and Gene Analysis", the molecular diagnostic automatic analyzer capable of performing a complex DNA microarray) comprises a device frame; A block for a polymerase chain reaction (PCR) in which the DNA chip is mounted, the block being installed on the frame for the device; Real-time PCR quantitative detection means for detecting in real time a fluorescence signal generated from a sample sample amplified in the DNA chip disposed in the PCR chain; And DNA microarray analysis means for detecting and analyzing a fluorescence signal generated by hybridizing a sample sample gene-amplified in the DNA chip disposed in the PCR chain with a target gene attached to the surface of the DNA chip (PCR) and DNA (microarray) analysis.

Korean Patent No. 10-1302353

SUMMARY OF THE INVENTION An object of the present invention is to provide a device for automatically supplying a solution to a lab-on-a-chip and reading a gene for the solution.

It is an object of the present invention to provide a gene discrimination apparatus which comprises a unit for supplying a solution to a lab-on-a-chip, a structure for performing gene reading for the solution, It is to be miniaturized.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

To achieve the above object, according to the present invention, there is provided a wrap-on-a-chip in which a plurality of plate members having different shapes are laminated and a plurality of kinds of solutions are introduced, mixed, and discharged; An operation mechanism module including a tray for fixing the lab-on-a-chip to an upper surface thereof, and a gene reading unit for performing electrochemical reading of genes introduced into the lab-on-a-chip; A solution supply module located in a direction of a lower surface of the tray and having a plurality of scans, and supplying solution to the lab-on-a-chip by operating each of the plurality of scans; A hybridization module that generates a reading solution to be read and transfers the reading solution to the operation mechanism module so that the reading solution is provided to the lab-on-a-chip; And the read solution is supplied to the lab-on-a-chip, and a solution stored in each of the plurality of scans is supplied to the lab-on-a-chip to perform electrochemical readout of the genes in the read solution, A solution supply module, and a control unit for performing control on the hybridization module.

In the embodiment of the present invention, the operation mechanism module may include a cover part covering the tray, a heating part coupled to the cover part and providing heat energy to the wrap-on-the-hand chip, On chip for controlling the flow of the read-out solution introduced into the lab-on-a-chip, and a control unit for controlling the operation of the lab- And a readout solution supply port for supplying the readout solution to the lab-on-a-chip.

In the embodiment of the present invention, the valve pressurizing portion may include a first pressurizing rod that pressurizes or releases a portion of the valve portion, a second pressurizing portion that pressurizes or releases the other portion of the valve portion, And a pressurizing operation portion for selectively operating the first pressurizing bar or the second pressurizing bar.

In the embodiment of the present invention, the gene reading unit includes a detecting electrode unit contacting the electrode unit provided in the lab-on-a-chip to energize the solution flowing in the lab-on-a-chip, a current sensed by the sensing electrode unit And a change measuring unit for measuring a change value of the change signal and transmitting the change signal to the control unit.

In the embodiment of the present invention, the solution supply module may include: a first scanning unit for supplying a washer fluid to the lab-on-a-chip; a second scanning unit for supplying a buffer solution to the lab-on-a-chip; A position adjusting section for adjusting a position of the first scanning section, the second scanning section and the discharge liquid storage section, and a position adjusting section for adjusting the position of the pushing part of the first scanning section, And a pushing moving part for selectively pushing and moving the pushing part.

In the embodiment of the present invention, the position adjustment unit may include a support for supporting and supporting the first scan unit, the second scan unit, and the discharge liquid storage unit, and a guide for guiding the support unit to move up and down, And a support pedestal driving part coupled to the support pedestal and providing power to move the support pedestal up and down.

In the embodiment of the present invention, the pushing unit may include a first pushing portion for pushing and moving the pushing portion of the first scanning portion, a second pushing portion for pressing and moving the pushing portion of the second scanning portion, A first conveying screw which passes through the body of the pusher pushing portion and rotates to move the first pusher portion while being threaded through the body of the second pusher portion and rotates to move the second pusher portion A second conveyance screw, and a screw motor unit for rotating the first conveyance screw and the second conveyance screw.

In one embodiment of the present invention, the hybridization module is provided with at least one kind of first solution containing a gene, and a control solution for controlling the acidity or salinity of the first solution is supplied to perform hybridization, A pressure cylinder connected to the hybridization storage unit for pressurizing the reading solution inside the pressure cylinder by movement of a pressure piston combined with the pressure cylinder, a pressure cylinder connected to the pressure piston, And a linear motion motor that is connected to the piston moving part and causes the piston moving part to reciprocate linearly.

In a preferred embodiment of the present invention, the fluid supply module, the hybridization module, and the frame load plate on which the control unit is installed, and the lower surface of the upper plate, And a frame part having a frame column that engages with an upper surface of the lower plate.

To achieve the above object, according to the present invention, there is provided a wrap-on-a-chip in which a plurality of plate members having different shapes are laminated and a plurality of kinds of solutions are introduced, mixed, and discharged; An operation mechanism module including a tray for fixing the lab-on-a-chip to an upper surface thereof, and a gene reading unit for performing electrochemical reading of genes introduced into the lab-on-a-chip; A solution supply module located in a direction of a lower surface of the tray and having a plurality of scans, and supplying solution to the lab-on-a-chip by operating each of the plurality of scans; A hybridization module that generates a reading solution to be read and transfers the reading solution to the operation mechanism module so that the reading solution is provided to the lab-on-a-chip; The read solution is supplied to the lab-on-a-chip and a solution stored in each of the plurality of scans is supplied to the lab-on-a-chip to perform an electrochemical readout of the genes in the read solution, A controller for controlling the supply module and the hybridization module, and a display unit for displaying an electrochemical readout result of the gene reading unit on a screen.

In an embodiment of the present invention, the apparatus further includes a memory unit for storing an electrochemical reading result performed by the gene reading unit, and a wireless communication unit for transmitting an electrochemical reading result performed by the gene reading unit to an external electronic device can do.

In an embodiment of the present invention, the external electronic device may be any one or more of a smart phone, a tablet PC, a computer, and a video device.

According to another aspect of the present invention, there is provided a method of manufacturing a semiconductor device, comprising: i) fixing the lab-on-a-chip to the tray; ii) bringing the cover portion of the operating mechanism module into close contact with the tray so that a readout solution supply port is connected to the first inlet of the lab-on-a-chip; on-the-fly chip, iii) the plurality of syringes move to connect the first scan portion to the second inlet of the lab-on-a-chip, the second scan portion to the third inlet of the lab- On-chip chip; iv) flowing the readout solution discharged from the hybridization module through the readout solution supply port to the first inlet portion; v) the reading solution is stopped from flowing inside the lab-on-a-chip, receiving thermal energy from the heat treatment provided in the operation mechanism module, and performing PCR; vi) performing the electrochemical reading through the electrode portion of the lab-on-a-chip connected to the gene reading portion, wherein the reading solution in which the step v) is performed is performed; And vii) displaying an electrochemical readout performed by the gene reading unit.

In an embodiment of the present invention, in the step vi), the reading solution flows sequentially while mixing with the washer fluid supplied from the first scanning part and the buffer fluid supplied from the second scanning part, and flows to the electrode part can do.

The effect of the present invention with such a structure as described above is that the plural solutions are automatically supplied to the lab-on-a-chip, the gene reading for the solution to be read is automatically performed, The read time can be reduced.

Further, the effect of the present invention can be attained by making each of the apparatuses necessary for gene reading using the lab-on-a-chip included in one apparatus to miniaturize the gene discrimination apparatus, thereby improving the mobility of the gene discrimination apparatus, It is possible to increase it.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

1 is a perspective view of an outside of a gene discrimination apparatus according to an embodiment of the present invention.
FIG. 2 is a perspective view illustrating an example of use of a gene discrimination apparatus according to an embodiment of the present invention.
FIG. 3 is a perspective view showing each configuration of a gene discrimination apparatus according to an embodiment of the present invention.
FIG. 4 is a perspective view of each configuration of a gene discrimination apparatus according to an embodiment of the present invention. FIG.
5 is a front view of the inside of the gene discrimination apparatus according to an embodiment of the present invention.
6 is a rear view of the inside of the gene discrimination apparatus according to an embodiment of the present invention.
7 is a side view of the inside of the gene discrimination apparatus according to an embodiment of the present invention.
8 is a perspective view of a lab-on-a-chip according to an embodiment of the present invention.
9 is a plan view of a lab-on-a-chip according to an embodiment of the present invention.
10 is an exploded perspective view of a lab-on-a-chip according to an embodiment of the present invention.
11 is a plan view of a channel plate according to an embodiment of the present invention.
12 is a plan view of a top plate according to an embodiment of the present invention.
13 is a plan view of a bottom plate according to an embodiment of the present invention.
14 is a plan view of a mixer plate and a mixer cover according to an embodiment of the present invention.
15 is a plan view of a valve plate and a valve cover according to an embodiment of the present invention.
FIG. 16 is a plan view of a reading passage plate and a reading plate according to an embodiment of the present invention. FIG.
17 is a cross-sectional view of a lab-on-a-chip in which a filter is closely contacted according to an embodiment of the present invention.
18 is a cross-sectional view of a mixer according to an embodiment of the present invention.
19 is a sectional view of a valve unit according to an embodiment of the present invention.
20 is a perspective view of an operating mechanism module according to an embodiment of the present invention.
21 is a plan view of an operating mechanism module according to an embodiment of the present invention.
22 is an image of an operating mechanism module according to an embodiment of the present invention.
23 is a perspective view of an operation mechanism module in a state that a cover is opened according to an embodiment of the present invention.
24 is an image of a gene reading unit according to an embodiment of the present invention.
25 is a front view of a first pressure bar and a second pressure bar according to an embodiment of the present invention.
26 is a perspective view of a scanning unit and a position adjusting unit according to an embodiment of the present invention.
27 is a perspective view of a plunger according to an embodiment of the present invention.
28 is an image of a scan unit according to an embodiment of the present invention.
29 is a perspective view of a hybridization module according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" (connected, connected, coupled) with another part, it is not only the case where it is "directly connected" "Is included. Also, when an element is referred to as "comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

2 is a perspective view of an example of the use of a gene discrimination apparatus according to an embodiment of the present invention, and FIG. 3 is a view showing the arrangement of a gene discrimination apparatus according to an embodiment of the present invention. FIG. 2 is a perspective view showing each configuration of a gene discrimination apparatus according to an embodiment. FIG.

FIG. 4 is a perspective view of a gene discrimination apparatus according to an embodiment of the present invention, and FIG. 5 is a front view of the interior of the gene discrimination apparatus according to an embodiment of the present invention. FIG. 2 is a rear view of the inside of the gene discrimination apparatus according to an embodiment of the present invention. FIG. 7 is a side view of the interior of the gene discrimination apparatus according to an embodiment of the present invention.

As shown in FIGS. 1 to 7, the gene discrimination apparatus of the present invention comprises: a lab-on-a-chip 1000 formed by stacking a plurality of plate materials having different shapes, and in which a plurality of kinds of solutions are introduced, mixed and discharged; An operation mechanism module 2000 including a tray 2200 for fixing the lab-on-a-chip 1000 to the upper surface thereof, and a gene reading unit 2100 for performing electrochemical reading on the gene introduced into the lab- ); A solution supply module 3000 located in the lower surface direction of the tray 2200 and having a plurality of scan portions and operating each of the plurality of scan portions to supply the solution to the lab-on-a-chip 1000; A hybridization module 4000 that generates a read solution to be read and transfers the read solution to the operation mechanism module 2000 so that a read solution is provided to the lab-on-a-chip 1000; And a read-out solution is supplied to the lab-on-a-chip 1000, and a solution stored in each of the plurality of scan portions is supplied to the lab-on-a-chip 1000 to perform an electrochemical reading on the gene in the read- And a control unit 6000 for performing control on the solution supply module 3000 and the hybridization module 4000.

2 (a) shows a state in which the cover part 2300 and the cover part case 5220 coupled to the cover part 2300 are open. FIG. 2 (b) And a plurality of lab-on-a-chip chips 1000 are stored in the package.

As shown in FIG. 1 and FIG. 2, the gene discrimination apparatus of the present invention has respective components installed therein, and a case 5200 may be provided on the outside in order to protect the components. The tray 2200 is configured to expose a portion of the upper surface of the case 5200 when the cover portion 2300 is opened and the lab-on-a-chip 1000 can be positioned on the tray 2200. When the cover part 2300 is closed and a switch is pressed, gene reading can be automatically performed.

On the upper surface of the case 5200, a storage part 5230 is provided to store a plurality of the lab-on-a-chip 1000 in the storage part 5230, and the lab-on-a-chip 1000 can be used immediately if necessary.

3 to 7, an operation mechanism module 2000 is provided adjacent to an upper surface of the case 5200, and a solution supply module 3000 is provided to supply a solution to the lower end of the operation mechanism module 2000. [ A hybridization module 4000 may be installed apart from the solution supply module 3000 and a control unit 6000 may be installed between the solution supply module 3000 and the hybridization module 4000.

One of the objects of the present invention is to provide mobility and convenience by installing all the structures capable of performing gene reading in a small-sized space, so that it can be realized with the above-described installation structure.

The lab-on-a-chip 1000 is connected to the gene discrimination apparatus of the present invention to perform gene reading. Hereinafter, the lab-on-a-chip 1000 of the present invention will be described.

FIG. 8 is a perspective view of a lab-on-a-chip 1000 according to an embodiment of the present invention, FIG. 9 is a plan view of a lab-on-a-chip 1000 according to an embodiment of the present invention, On-a-chip 1000 according to an example.

(The lab-on-a-chip 1000 in FIGS. 8-10 is inverted in shape for being fixed to the tray 2200.)

FIG. 11 is a plan view of a channel plate according to an embodiment of the present invention, FIG. 12 is a plan view of an upper plate according to an embodiment of the present invention, and FIG. 13 is a plan view of a lower plate according to an embodiment of the present invention.

Figs. 8 and 9 are diagrams showing a state in which the respective components are coupled. Fig.

In Figs. 8 and 9, each component is indicated by a solid line, because each plate is a transparent or translucent material, and each component is projected and visually confirmed.

(The channel in the present invention means a channel formed by perforation of the channel plate 1110 and having upper and lower openings. The channel in the present invention is a channel in which the channel plate 1110, the upper plate 1120, Quot; means a flow path of the liquid formed by the close contact of the liquid material 1130).

As shown in FIGS. 8 to 13, the lab-on-a-chip 1000 for gene reading of the present invention is a lab-on-a-chip 1000 formed by laminating a plurality of plate materials having different shapes, A channel part 1100 for providing a flow path; A valve portion 1400 for providing a valve passage to the liquid flowing through the channel portion 1100 and closing or opening the valve passage; A mixer 1300 provided in the channel unit 1100 and adapted to flow the liquid in a direction perpendicular to the flow direction of the flow path; A filter 1200 coupled to the channel portion 1100 and contacting the channel; And a reading unit 1500 that provides a readout channel 1511 to the liquid flowing through the channel unit 1100 and reads a gene that passes through the readout channel 1511. [

Here, the mixer 1300 is provided with at least one vortex space 1140 for turbulently moving the liquid flowing in the passage, the vortex space 1140 has a predetermined shape for vortex generation, The bubble generator 1200 can remove bubbles of liquid flowing in the flow path.

The channel portion 1100 is in close contact with the upper surface of the channel plate 1110 and the upper surface of the channel plate 1110. The upper surface of the channel portion 1100 is a plate member in which the upper plate mixer hole portion and the upper plate channel 1127 are punched, And a lower plate 1130 which is a sheet material adhered to the lower surface of the channel plate 1110. [

A filter 1200 may be attached to the top plate channel 1127 of the top plate 1120.

At least one of the filters 1200 may be provided in the channel portion 1100.

A bonding portion 1110 for attaching the filter 1100 may be formed between the upper plate channel 1241 and the filter 1100. This will be described later in detail.

The filter 1200 may be a hydrophobic material.

The reading solution flowing along the flow path may comprise protein particles, and the reading solution may be a liquid in which one or more hydrophilic liquids are mixed with water. Accordingly, the filter 1200 may have a function of passing gas without passing water and a hydrophilic liquid. Accordingly, the filter 1200 can perform a function of separating and removing only the gas using a hydrophobic material.

The filter 1200 may be at least one material selected from the group consisting of polytetrafluoroethylene (PTFE), polyethylene terephthalate (PET), and polyvinyl chloride.

The channel part 1100 is formed by the channel plate 1110, the upper plate 1120 and the lower plate 1130 in close contact with each other as described above. However, the present invention is not limited to this, The channel plate 1110 and the lower plate 1130 may have the same shape as that of the plate. Even in this case, the upper plate 1120 can be closely contacted to the surface on which the grooves are formed, and a flow path through which the test liquid flows can be formed.

The channel portion 1100 further includes a mixer plate 1310 which is in close contact with the upper surface of the upper plate 1120 and is a plate material in which a mixer plate hole portion is perforated, and the vortex space 1140 includes a channel plate mixer hole 1111 The upper plate mixer hole 1121 and the mixer plate hole 1311 may be combined with each other.

(As shown in FIG. 12, in an embodiment of the present invention, a plurality of upper plate mixer holes 1121 may be formed in two rows in the upper plate 1120.)

14 is a plan view of a mixer plate 1310 and a mixer cover 1320 according to an embodiment of the present invention. FIG. 14A is a view of the mixer plate 1310, and FIG. 7B is a view of the mixer cover 1320. FIG.

One or more mixers 1300 may be provided in the channel unit 1100.

The mixer 1300 may further include a mixer cover 1320 which is in close contact with the upper surface of the mixer plate 1310.

In the mixer 1300, a liquid containing a specific substance and a wash liquid may be mixed.

The specific substance may be a biomaterial such as DNA, and the wash liquid may prevent the separated DNA from recombining.

The mixer 1300 may have one or more vortex spaces 1140 and the vortex space 1140 may have a predetermined shape for vortex generation.

The liquid flows from one side 1111-a of the channel plate mixer hole to one mixer hole 1121-a through one upper mixer hole 1121-a to a mixer plate hole 1311 connected to one upper mixer hole 1121-a, It may flow from the planar hole 1311 to another upper plate mixer hole 1121-b and to the other side 1111-b of the channel plate mixer hole.

This will be described in detail in the rear end mixing method.

15 is a plan view of a valve plate 1410 and a valve cover 1420 according to an embodiment of the present invention. FIG. 15A is a view of the valve plate 1410, and FIG. 15B is a view of the valve cover 1420. FIG.

The channel portion 1100 includes a top plate hole 1122 and the valve portion 1400 is a plate member having a valve plate hole 1411 formed into a predetermined shape and a valve plate hole 1411 is formed on the top plate hole 1122 And a valve cover 1420 that is a plate member that is in close contact with the upper surface of the valve plate 1410. The valve cover 1420 is a plate member that is in close contact with the upper surface of the valve plate 1410. [

The channel unit 1100 includes the upper plate 1120, the channel plate 1110 and the lower plate 1130 and the upper plate 1122 may be provided on the upper plate 1120.

At this time, the valve passage can be closed or opened by pressing or releasing the pressure against the valve cover 1420.

The valve cover 1420 may be formed of a silicone material so as to have excellent stretchability with respect to volume or area change.

The channel part 1100 may have a plurality of upper plate holes 1122. Here, a plurality of upper plate holes 1122 may be provided in the upper plate 1120, depending on the configuration of the channel unit 1100.

The upper plate 1120 has a plurality of upper plate holes 1122 and the fluid flowing through the channel flows from one side of the flow path through the first upper plate hole 1122a to the valve plate hole 1411 And can be discharged to the other side of the flow path from the valve plate hole 1411 through the second upper plate hole 1122b.

As shown in FIG. 10, the flow path may be disconnected in a predetermined section, and the first upper plate hole 1122a and the second upper plate hole 1122b may be connected to the respective flow paths on both sides of the divided flow path section. have. The liquid that flows into the valve inlet flow path 1113 and then flows through one side of the flow path can flow to the valve plate hole 1411 through the first upper plate hole 1122a. The liquid that has flowed into the valve plate hole 1411 is discharged to the other side of the flow path from the valve plate hole 1411 by the pressure supplied to the valve inlet flow path 1113 and can continue to flow through the flow path. At this time, when the valve cover 1420 which is in close contact with the valve plate hole 1411 is pressed by a predetermined mechanism, the path of the liquid flowing from the one side of the flow path to the other side of the flow path is closed .

The area of the valve plate hole 1411 located on the first upper plate hole 1122a may be different from the area of the portion located on the second upper plate hole 1122b.

As described above, when the path of the liquid flowing from the one side of the flow path to the other side of the flow path is closed, the pressing region of the valve cover 1420 which is in close contact with the valve plate hole 1411 can be specified. The area of the valve plate hole 1411 located on the first upper plate hole 1122a may be smaller than the area of the portion located on the second upper plate hole 1122b.

The pressing portion of the valve cover 1420 which is in contact with the valve plate hole 1411 can be a portion located on the first upper plate hole 1122a.

16 is a plan view of the reading flow path plate 1510 and the reading plate 1520 according to the embodiment of the present invention. FIG. 16A is a view of the readout flow path plate 1510, and FIG. 16B is a view of the readout plate 1520. FIG.

The reading unit 1500 includes a readout channel plate 1510 formed by punching and forming the readout channel 1511 and a readout plate 1520 which is in close contact with the upper surface of the readout channel plate 1510, ), ≪ / RTI > The electrode portion may include a counter electrode 1521, a reference electrode 1522, and a working electrode 1523.

As shown in FIG. 16B, a plurality of counter electrodes 1521 are arranged on the right side of the reading plate 1520, and the counter electrodes 1521 can be connected to an external device to perform an electric signal acquisition function.

The reference electrode 1522 is connected to each of the working electrodes 1523 as shown in FIG. 16B, and can perform a function of supplying a current to the working electrode 1523.

16 (b), a plurality of working electrodes 1523 are arranged on the left side of the reading plate 1520, and each of the working electrodes 1523 is connected to the reference electrode 1522 and is connected to each of the working electrodes 1523 Different probe DNAs can be planted.

The liquid sufficiently mixed in the mixer 1300 and passed through the hybridization flow path 1112 flows into the readout flow path 1511 from one side of the channel portion 1100 connected to the readout flow path 1511, ) Can be carried out by the electrode portion of the electrode. The liquid that has passed through the readout flow path 1511 can flow to the flow path side of the channel portion 1100 connected to the readout flow path 1511 from the readout flow path 1511.

The liquid may then be discharged to the outlet 1126.

As shown in FIG. 16, the electrode portion may be formed of a portion where a plurality of electrodes are arranged and a single electrode having a predetermined shape.

Here, the single electrode may have a protruding portion so as to be in contact with the liquid passing through the readout passage 1511, and energizing the liquid passing through the readout passage 1511 through the protruded portion .

The plurality of electrodes arranged may be provided in the reading plate 1520 such that the respective ends of the plurality of electrodes are in contact with the liquid passing through the reading passage 1511. As a result, the plurality of electrodes can conduct electricity to the liquid passing through the readout flow path 1511.

The positive electrode (+) may be in contact with a plurality of arranged electrodes, and the negative electrode (-) may be in contact with a single electrode. On the contrary, the negative electrode (-) is brought into contact with a plurality of arranged electrodes, and the positive electrode (+) is brought into contact with a single electrode.

In the liquid that has passed through the readout channel 1511 and made in contact with the electrode portion, the gene reading using the electrochemical detection signal can be performed.

At this time, the electrochemical detection can be used in both a labeling method and a non-labeling method. This will be described later in detail.

The lab-on-a-chip 1000 for reading genes of the present invention may further include an inflow portion into which the liquid flows or a discharge portion 1126 through which the liquid is discharged.

3, the first inlet 1123, the second inlet 1124, the third inlet 1125, and the outlet 1126 are connected to the lab-on-a-chip 1000 for gene reading of the present invention .

The cap 1150 can be coupled to the second inflow portion 1124, the third inflow portion 1125, or the discharge portion 1126 such that the inflow or outflow of liquid can be performed by the scanning device.

At this time, the cap 1150 may be formed of rubber or silicon. However, the present invention is not limited to this, and it is possible to use any material that does not leak liquid when the needle passes.

The reading solution containing the gene to be read may be introduced into the first inlet 1123 and the washer fluid may be introduced into the second inlet 1124. [ The buffer solution may be introduced into the third inlet 1125.

The washer fluid joins with the readout solution in which the gene amount has been increased by the polymerase chain reaction (PCR), and consequently, the concentration lowering effect can be given to the readout solution having the increased concentration.

The buffer solution functions to enable gene reading using an electrochemical detection signal for the reading solution, and can flow to the reading part 1500 by mixing with the reading solution.

The read-out read-out solution can be discharged to the outside through the discharge portion 1126.

The channel plate 1110, the top plate 1120, the bottom plate 1130, the mixer plate 1310, or the mixer cover 1320 may be in the form of a film.

Then, the valve plate 1410, the reading channel plate 1510, or the reading plate 1520 may be in the form of a film.

Since the channel is formed by laminating the plate materials in the form of a film, it is easy to reduce the channel size and the shape of the channel can be varied.

The channel plate 1110, the upper plate 1120, the lower plate 1130, the mixer plate 1310, the mixer cover 1320, the valve plate 1410, the reading channel plate 1510 or the reading plate 1520 are formed of synthetic resin .

The synthetic resin forming the channel plate 1110, the upper plate 1120, the lower plate 1130, the mixer plate 1310, the mixer cover 1320, the valve plate 1410, the reading channel plate 1510 or the reading plate 1520 Polyimide (PI), polyethylene terephthalate (PET), polypropylene (PP), ABS resin (Acrylonitrile Butadiene Styrene), polycarbonate (PC), polystyrene (SAN), ethylene vinyl acetate (EVA), polyamide (Nylon) resin (Polyamide, PA), polyethylene terephthalate May be one or more synthetic resin materials.

The channel plate 1110, the upper plate 1120, the lower plate 1130, the mixer plate 1310, the mixer cover 1320, the valve plate 1410, the reading channel plate 1510 or the reading plate 1520 are formed of metal .

The channel plate 1110, the upper plate 1120, the lower plate 1130, the mixer plate 1310, the mixer cover 1320, the valve plate 1410, the reading channel plate 1510 or the reading plate 1520 are formed of glass .

In the embodiment of the present invention, the channel plate 1110, the upper plate 1120, the lower plate 1130, the mixer plate 1310, the mixer cover 1320, the valve plate 1410, the reading channel plate 1510, (1520) are formed of the materials listed above, the present invention is not limited thereto.

Hereinafter, the lab-on-a-chip 1000 for gene reading having the hybridization flow path 1112 will be described.

A lab-on-a-chip 1000 having a hybridization channel 1112 is a lab-on-a-chip 1000 formed by laminating a plurality of plates having different shapes, A channel unit 1100 for providing the data; A valve portion 1400 for providing a valve passage to the liquid flowing through the channel portion 1100 and closing or opening the valve passage; A mixer 1300 provided in the channel unit 1100 and adapted to flow the liquid in a direction perpendicular to the flow direction of the flow path; A filter 1200 coupled to the channel portion 1100 and contacting the channel; A hybridization channel 1112 as a predetermined section of the channel in contact with the filter 1200 and performing hybridization; And a reading unit 1500 that provides a readout channel 1511 to the liquid flowing through the channel unit 1100 and reads a gene that passes through the readout channel 1511. [

Here, the mixer 1300 is provided with at least one vortex space 1140 for turbulently moving the liquid flowing in the passage, the vortex space 1140 has a predetermined shape for vortex generation, The bubble generator 1200 can remove bubbles of liquid flowing in the flow path.

The hybridization flow path may have a zigzag shape.

The hybridization channel may be a channel formed by coupling the channel plate hybrid channel 1112 and the top plate channel 1127 as shown in FIGS.

Hybridization can be defined as the process of heat-treating two double-stranded DNAs of different origins into a single strand and then making the double-stranded DNA hybridized.

In order to facilitate hybridization, the hybridization flow path 1112 has a zigzag shape and may be formed to have a wider area than other flow path portions. This can facilitate the hybridization by reducing the velocity of the flowing liquid. If the velocity of the flowing liquid is reduced, the probability of contact between single strands of DNA can increase.

The filter 1200 may be positioned to be able to contact any portion of the entire flow path, but it may be desirable to be positioned to contact the hybridization flow path 1112 where the flow rate is slow to increase the bubble removal rate.

In an embodiment of the present invention, the filter 1100 may be in contact with the liquid flowing through the top plate channel 1241, which is part of the hybridization flow path.

Hereinafter, the lab-on-a-chip 1000 for reading genes having the chamber 1116 will be described.

A lab-on-a-chip 1000 having a chamber 1116 is a lab-on-a-chip 1000 formed by laminating a plurality of plates having different shapes, A channel unit 1100; A valve portion 1400 for providing a valve passage to the liquid flowing through the channel portion 1100 and closing or opening the valve passage; A chamber 1116 for temporarily storing fluid as a predetermined section of the flow path; A mixer 1300 provided in the channel unit 1100 and adapted to flow the liquid in a direction perpendicular to the flow direction of the flow path; A filter 1200 coupled to the channel portion 1100 and contacting the channel; And a reading unit 1500 that provides a readout channel 1511 to the liquid flowing through the channel unit 1100 and reads a gene that passes through the readout channel 1511. [

Here, the mixer 1300 may include at least one vortex space 1140 to turbulently move the liquid flowing in the passage, and the vortex space 1140 may have a predetermined shape for vortex generation . The filter 1200 can remove bubbles of liquid flowing in the channel, and a polymerase chain reaction (PCR) can be performed in the chamber 1116.

The chamber 1116 may be a zone of zigzag shape in the flow path.

The chamber 1116 functions to temporarily store the fluid, and thus may be formed in various shapes. In the embodiment of the present invention, the chamber 1116 is formed in a predetermined section of the channel, but the present invention is not limited thereto. The channel 1116 may be connected to one side of the channel and the other side of the channel, As shown in FIG.

The lab-on-a-chip 1000 for gene reading of the present invention is formed by piercing the channel part 1100 in a shape surrounding the valve part 1400 and the chamber 1116, And a heat transfer prevention trough 1117 that blocks transmission to the rest of the portion 1100.

Heat is supplied only to the chamber 1116 and a part of the valve portion 1400 by the heat transfer preventing tread 1117 and other portions of the channel portion 1100 are not affected by heat so that in the chamber 1116, And the liquid flowing through the other part of the channel part 1100 except for the chamber 1116 can flow while preventing deterioration due to excessive heat.

In addition, the heat supplied to the chamber 1116 by the heat-transfer preventing tread 1117 is not dispersed to the surroundings, so that the speed and efficiency of the polymerase chain reaction can be improved.

Hereinafter, a lab-on-a-chip 1000 for gene reading having a heat plate will be described.

A lab-on-a-chip 1000 having a heat plate portion is a lab-on-a-chip 1000 (Lap on a chip) formed by laminating a plurality of plates having different shapes, (1100); A valve portion 1400 for providing a valve passage to the liquid flowing through the channel portion 1100 and closing or opening the valve passage; A mixer 1300 provided in the channel unit 1100 and adapted to flow the liquid in a direction perpendicular to the flow direction of the flow path; A filter 1200 coupled to the channel portion 1100 and contacting the channel; A reading unit 1500 that provides a reading channel 1511 to the liquid flowing through the channel unit 1100 and reads a gene that passes through the reading channel 1511; And a heating plate for heating or cooling a part of the upper surface of the channel part 1100 or a part of the lower surface of the channel part 1100.

Here, the mixer 1300 may include at least one vortex space 1140 to turbulently move the liquid flowing in the passage, and the vortex space 1140 may have a predetermined shape for vortex generation . The filter 1200 can remove bubbles of liquid flowing in the flow path.

The heat plate portion can contact the remaining portion of the top plate 1120 except for the position where the valve cover 1420 is in close contact when the top plate 1120 is contacted.

10, 12 and 15, the valve plate 1410 includes a first valve plate hole 1411-a and a second valve plate hole 1411-b, and the upper plate 1120 includes a first valve plate hole 1411- A hole 1122a, a second upper plate hole 1122b, a third upper plate hole 1122c, and a fourth upper plate hole 1122d.

The first valve plate hole 1411-a, the first upper plate hole 1122a and the second upper plate hole 1122b are used to control the flow of the liquid flowing in the valve inlet flow path 1113 toward the chamber 1116 . The path of the liquid flowing in the direction of the valve discharge passage 1114 from the chamber 1116 side by using the second valve plate hole 1411-b, the third upper plate hole 1122c and the fourth upper plate hole 1122d is Can be controlled.

When the valve cover 1420 located on the first valve plate hole 1411-a and the second valve plate hole 1411-b is pressed, the liquid containing DNA can be temporarily stored in the chamber 1116.

When the liquid containing DNA is temporarily stored in the chamber 1116, a polymerase chain reaction (PCR) can be performed by heating by contact of the heat plate and cooling by releasing the contact of the heat plate.

The valve plate 1410 is provided with a first valve plate hole 1411-a and a second valve plate hole 1411-b and a fluid flowing through the first valve plate hole 1411-a and a second valve plate hole 1411- -b) may have different flow direction.

The heat plate portion may be formed of a thin metal plate.

At this time, the heat plate portion can generate heat by energizing the metal thin plate itself. Alternatively, a heat ray may be provided on a part of the thin metal plate to generate heat.

17 is a sectional view of a lab-on-a-chip 1000 to which a filter 1200 according to an embodiment of the present invention is closely attached.

17, the upper plate 1120, the channel plate 1110 and the lower plate 1250 are stacked and the filter 1200 can be brought into contact with the upper plate channel 1241 of the upper plate 1120. As shown in FIG. Accordingly, the filter 1200 is in close contact with the upper portion of the part of the hybridization flow path, and is in direct contact with the liquid flowing through the flow path, thereby removing bubbles present in the liquid.

At this time, a bonding portion for bonding may be formed between a portion of the upper plate 1120 on which the filter 1200 and the upper plate channel 1127 are formed. In order to contact the liquid and the filter 1200, the same rudder as the formation of the upper plate channel 1120 may be formed. Further, the bonding portion may be formed of a double-sided tape

Hereinafter, a mixing method in the mixer 1300 will be described.

18 is a cross-sectional view of a mixer 1300 according to an embodiment of the present invention.

(The arrow in Fig. 18 indicates the flow path of the liquid).

18, the liquid flowing through the channel plate 1110 passes through one of the upper plate mixer holes 1121-a from one side 1111-a of the channel plate mixer hole as one side of the channel plate 1110 And then flows through the other mixer hole 1121-b from the mixer plate hole 1311 to the channel side of the channel plate 1220 through the other upper plate mixer hole 1121- And can flow to the other side 1111-b of the channel plate mixer hole. At this time, the upper plate mixer hole 1122 can be formed into a predetermined shape such as a cross shape or a straight shape, and the liquid flowing through the upper plate mixer hole 1122 can turbulently move.

As shown in FIG. 18, one upper plate mixer hole 1121, a mixer plate hole 1311 corresponding to the upper plate mixer hole 1121, and a channel plate mixer hole (corresponding to one upper plate mixer hole 1121) 1111 may be combined to form an eddy space 1230. At least one of the vortex spaces 1140 may be formed.

When the liquid flows, the mixer 1300 may be positioned so that liquid passes first through the mixer 1300 rather than through the hybridization flow path 1112. In the mixer 1300, the vortex motion for mixing is performed a plurality of times, so that bubble generation can be increased. The mixer 1300 may be positioned such that the liquid passes first through the mixer 1300 rather than through the hybridization channel 1112 in order to efficiently remove the generated bubbles and test or inspect.

11, the vortex space 1140 can be arranged in two rows, the vortex space 1140 on the right side in Fig. 11 is located in one row, and the vortex space 1140 in the other row Lt; RTI ID = 0.0 > 1140 < / RTI > Accordingly, arrows are displayed upward to display the liquid flowing into the vortex space 1140 of the other row.

In the embodiment of the present invention, it is explained that the upper plate mixer hole 1121 is formed in a cross shape, a straight shape, and the like, but it is not limited to this, and various shapes can be formed.

Hereinafter, an operation method of the valve for the lab-on-a-chip 1000 according to the present invention will be described.

19 is a sectional view of a valve portion 1400 according to an embodiment of the present invention.

Fig. 19 (a) is a view showing a state in which the valve cover 1420 is not pressed, and Fig. 6 (b) is a view showing a state in which the valve cover 1420 is pressed. (Arrows indicate whether or not the liquid flows.)

First, a readout solution can be introduced.

As shown in FIG. 19 (a), the introduced read solution may flow to the first valve plate hole 1411-a through the first top plate hole 1122-a by reaching the flow path interruption period 1115 . The reading solution can flow from the first valve plate hole 1411-a through the second upper plate hole 1122-b to the flow path again by the pressure provided to the valve inlet flow path 1113.

19B, the portion of the valve cover 1420 located on the first valve plate hole 1411-a and the second upper plate hole 1122-b is connected to the first pressure rod 2610, Or the second pressurizing bar 2620, the reading solution introduced into the valve inflow passage 1113 can be stopped at the passage interruption interval 1115.

When the pressurization of the valve cover 1420 located on the first valve plate hole 1411-a and the second upper plate hole 1122-b is released, the flow- Flow through the flow path may be possible.

Although the pressing portion of the valve cover 1420 is described as a valve cover 1420 located on the first valve plate hole 1411-a and the second upper plate hole 1122-b in the embodiment of the present invention, The present invention is not limited thereto and pressure may be applied to the valve cover 1420 located on the first valve plate hole 1411-a and the first upper plate hole 1122-a.

The flow path can be controlled on the same principle in the second valve plate hole 1411-b, the third upper plate hole 1122-c, and the fourth upper plate hole 1122-d.

Hereinafter, a gene reading method using the lab-on-a-chip 1000 of the present invention will be described.

The target liquid containing the target DNA for the purpose of gene reading, which has undergone the polymerase chain reaction process and the mixing process with another liquid, can be read through the reading unit 1500.

To do this, the probe DNA may be preliminarily planted on the working electrode 1523.

The reading target liquid in flow can contact the counter electrode 1521 and the working electrode 1523 of the reading plate 1520 while passing through the reading channel 1511.

At this time, the probe DNA which is present on any one of the working electrodes 1523 and capable of binding to the target DNA and the target DNA of the target liquid for reading can be combined. With such a coupling, the current value for each electrode of the reading plate 1520 is changed, and the target DNA type of the reading purpose liquid can be read according to the signal level indicating the degree of such change.

Hereinafter, each component of the gene discrimination apparatus of the present invention will be described.

3 to 7, the gene discrimination apparatus of the present invention includes a frame float plate 5110 on which an operation mechanism module 2000 is mounted on a top surface, a solution supply module 3000 and a hybridization module 4000 and a control unit 6000 and a frame part 5130 having a lower surface of the frame floating plate 5110 and a frame column 5130 engaging with the upper surface of the frame load plate 5120 5100).

In addition, the case 5200 may be combined with the frame 5100 serving as a skeleton to form the gene discrimination apparatus of the present invention.

FIG. 20 is a perspective view of an operation mechanism module 2000 according to an embodiment of the present invention, FIG. 21 is a plan view of an operation mechanism module 2000 according to an embodiment of the present invention, and FIG. An example of an operation mechanism module 2000 according to an example. 23 is a perspective view of the operation mechanism module 2000 with the cover part 2300 opened according to an embodiment of the present invention.

20-23, the operating mechanism module 2000 includes a cover portion 2300 that covers the tray 2200, a cover portion 2300 that couples to the cover portion 2300 and provides thermal energy to the lab- A cooling section 2500 for cooling the lab-on-a-chip 1000 by engaging with the cover section 2300 and rotating the fan, a cooling section 2500 for controlling the flow of the read- A valve portion 2600 for pressurizing or releasing the valve portion 1400 provided in the on-chip 1000 and a read-out solution supplying portion 265 for supplying the read-out solution transferred from the hybridization module 4000 to the lab- And a sphere 2800, as shown in Fig.

(In the above description of the lab-on-a-chip 1000, the heat plate portion may include the heat treatment 2400 or the cooling portion 2500.)

The cover portion 2300 is hinged to the upper surface of the frame floating plate 5110 or the case 5200 so that the cover portion 2300 can be opened and closed with respect to the tray 2200 by rotating around the hinge. When the cover portion 2300 is closed, the heat application 2400 contacts the outer surface of the lab-on-a-chip 1000 formed with the chamber 1116, the valve pressing portion 2600 is positioned on the valve portion 1400, The solution supply port 2800 may be connected to the first inlet to prepare for supplying the read-out solution to the lab-on-a-chip 1000. The cooling unit 2500 is coupled to the upper end of the heating unit 2400 so that the cooling unit 2500 can perform air cooling with the fan when the heating unit 2400 does not provide thermal energy.

Here, the heat treatment member 2400 may be formed of a Peltier element or a hot wire.

The valve pressing portion 2600 is provided with a first pressing rod 2610 for performing pressing or releasing of pressure on one portion of the valve portion 1400 and a second pressing rod 2610 for performing pressurization or pressure releasing to other portions of the valve portion 1400 And a pressurizing operation portion for selectively operating the second pressure bar 2620 and the first pressure bar 2610 or the second pressure bar 2620. [

The pressure actuating portion may include a first pressure actuator for linearly moving the first pressure bar 2610 and a second pressure actuator for linearly moving the second pressure bar 2620. [ The first pressure bar 2610 may be positioned on the first valve plate hole and the second pressure bar 2620 may be positioned on the second valve plate hole.

First, when the read-out solution flows into the chamber 1116 of the lab-on-a-chip 1000, the first pressure bar 2610 maintains the pressure release state against the first valve plate hole and the second pressure bar 2620 It is possible to perform pressurization to the two-valve plate hole. Then, when the readout solution is stored in the chamber 1116, the first pressurizing rod 2610 performs the pressurization to the first valve plate hole, and the polymerase chain reaction (PCR) for the readout solution in the chamber 1116 is performed .

Thereafter, after the polymerase chain reaction, the second pressurizing rod 2620 is released from pressure against the second valve plate hole, and the read solution in the state of gene amplification is mixed with the washer liquid and the buffer solution along the flow path, It can flow.

During the polymerase chain reaction, the thermal treatment (2400) can provide thermal energy into the chamber (1116). The temperature sensor 2400 may be equipped with a temperature sensor. The temperature sensor senses the temperature of the chamber 1116 in real time and transmits the sensed temperature to the control unit 6000. The control unit 6000 performs temperature control of the heating unit 2400 using the temperature of the sensed chamber 1116 .

In the above description, the heating unit 2400 is combined with the cover unit 2300 and brought into contact with the lab-on-a-chip 1000 to provide thermal energy. However, as shown in FIG. 5, 5110 to provide thermal energy to the chamber 1116. [ The cooling unit 2500 can be coupled to the heating unit 2400 combined with the frame floating plate 5110.

Each of the first pressing bar 2610 and the second pressing bar 2620 is provided with a ball bearing 2630 so that the ball bearing 2630 can perform a damping action when pressed.

The gene reading unit 2100 includes a detecting electrode unit 2110 and a detecting electrode unit 2110 which are in contact with electrode units provided in the lab-on-a-chip 1000 so as to energize the solution flowing in the lab- And a change measuring unit 2120 for measuring a change value of the current detected by the current measuring unit and transmitting the change signal to the controller 6000. [

The principle in which the electrochemical reading of the gene is performed by the gene reading unit 2100 may be the same as the electrochemical reading in the description of the lab-on-a-chip 1000 described above.

When the cover portion 2300 is closed, the detection electrode portion 2110 contacts the counter electrode 1521 provided in the lab-on-a-chip 1000 and the reference electrode 1522, and the detection electrode portion 2110 May transmit a change in current to the change measuring unit 2120. [ Thereafter, the change measuring unit 2120 transmits a change signal obtained by signaling the change of the current to the control unit 6000, and the control unit 6000 can analyze the change signal to determine the kind of the gene in the read solution. In this case, the control unit 6000 receives the reference gene information as a reference for gene discrimination, compares the gene of the reading solution with the reference gene information, and determines the genetic information of the reading solution based on the matching.

FIG. 24 is an image of the gene reading unit 2100 according to an embodiment of the present invention, and FIG. 25 is a graph showing the relationship between the first pressing rod 2610 and the second pressing rod 2620 according to an embodiment of the present invention. And FIG. 26 is a perspective view of a scanning unit and a position adjusting unit 3200 according to an embodiment of the present invention.

FIG. 27 is a perspective view of a plunger according to an embodiment of the present invention, and FIG. 28 is an image of a scan unit according to an embodiment of the present invention.

24 to 28, the solution supply module 3000 includes a first scanning unit 3110 for supplying a washer liquid to the lab-on-a-chip 1000, a second scanning unit 3110 for supplying a buffer solution to the lab- On-the-fly chip 1000, a liquid discharge unit 3130 for storing and storing the liquid discharged from the lab-on-a-chip 1000, a first scan unit 3110, a second scan unit 3120, A position adjusting section 3200 for adjusting the position of the first scanning section 3130 and a pushing moving section 3300 for selectively pressing and moving the pushing of the first scanning section 3110 or the second scanning section 3120 can do.

The position adjuster 3200 allows the respective scans to be close to the lab-on-a-chip 1000 and the needles of the first scans 3110 pass the cap of the second inlet to supply the washer fluid to the flowpath, The needle of the injection section 3120 passes through the cap of the third inlet to supply the buffer solution to the flow passage, the needle of the discharge solution storage section 3130 passes through the cap of the discharge section, .

The pushing unit 3300 sequentially presses the plunger of the first scanning unit 3110 and the plunger of the second scanning unit 3120 under the control of the control unit 6000, The buffer solution may be supplied from the second scanning portion 3120 to the lab-on-a-chip chip 1000 after the washer fluid is supplied from the lab-on-a-chip chip 1000 to the flow-

The position adjusting unit 3200 is coupled to a supporting base 3210 and a supporting base 3210 which are supported by being coupled with the first scanning unit 3110, the second scanning unit 3120 and the discharged liquid storage unit 3130, A support base guide portion 3220 for guiding the support base 3210 to move up and down and a support base drive portion 3230 for coupling with the support base 3210 and providing power to move the support base 3210 up and down.

The supporting rod guide portion 3230 includes a first rack gear 3231 which is engaged with the support base 3210 and has a thread formed on a part of the first rack gear 3232, a first rack gear 3231 spaced apart from the first rack gear 3231, A second rack gear 3232 in which a thread is formed so as to face the thread of the first rack gear 3231 and a second rack gear 3232 which meshes with the first rack gear 3231 and the second rack gear 3232, And a position adjustment motor unit 3233 for linearly reciprocating the rack gear 3231 and the support base 3210. [

The position adjustment unit 3200 includes a first position sensor, and can sense the position of the support base 3210 and transmit the sensed position to the control unit 6000. The control unit 6000 controls the position adjusting unit 3200 so that each scanning unit performs a linear movement to be connected to the lab-on-a-chip 1000 after the cover unit 2300 is closed and the switch is turned on Can be controlled.

The position adjustment unit 3200 may be fixed to the frame load plate 5120 and the support unit 3240 may be coupled between the support unit guide unit 3220 and the frame load plate 5120, 3220 may be supported.

The throttle moving part 3300 includes a first pushing part 3310 for pushing and moving the push of the first scanning part 3110 and a second pushing part for pushing and moving the pushing part of the second scanning part 3120 3320), a first conveying screw 3330 that passes through the body 3311 of the first pressurizing portion while being screwed and rotates to move the first pressurizing portion 3310, a body 3321 of the second pressurizing portion A second conveyance screw 3340 that passes through the second pushing portion 3320 while being screwed and rotates to move the second pushing portion 3320 and a screw motor portion (not shown) that rotates the first conveying screw 3330 and the second conveying screw 3340 3400, < / RTI >

The first conveying screw 3330 and the second conveying screw 3340 are formed with male threads on the outer circumferential surface and the through hole of the body 3311 of the first pushing portion and the penetrating portion of the body 3321 of the second pushing portion, The male thread of the first feed screw 3330 and the female screw thread of the penetration portion of the body 3311 of the first pushing portion mate with each other and the male thread of the second feed screw 3340 and the body 3321 of the second pushing portion, The female threads of the penetration portions can be matched.

The first abutting pressing portion 3310 performs a linear reciprocating motion by the rotation of the first feeding screw 3330 and the second abutting pressing portion 3320 is rotated by the rotation of the second feeding screw 3340, A reciprocating motion can be performed. In this case, the screw motor unit 3400 includes a first screw motor for rotating the first conveyance screw 3330 and a second screw motor for rotating the second conveyance screw 3340, The screw motor can be controlled to selectively move one of the first pushing press portion 3310 or the second pushing press portion 3320. [

And a second position sensor may be provided on the oscillating diaphragm 3300. The second position sensor may sense the position of the first pusher 3310 or the second pusher 3320 and may transmit the position of each pusher to the control unit 6000.

The amount of the washer fluid supplied by the first scanning unit 3110 can be determined by the change in the position of the first pusher 3310 and the position of the second scanning unit 3120 can be determined by the change in the position of the second pusher 3320. [ It is possible to control the supply amount of the washer fluid and the supply amount of the buffer solution by the operation of the scale shifting section 3300 by the control section 6000. [

The hybridization module 4000 includes a hybridization storage unit 4100 in which a first solution containing a gene is supplied and a control solution for controlling the acidity or salinity of the first solution is supplied to the hybridization module 4000, A pressurizing cylinder portion 4200 having a pressurizing cylinder 4210 connected to the hybridization storing portion 4100 and pressurizing the initial solution by the movement of the pressurizing piston 4220 combined with the pressurizing cylinder 4210, A piston moving part 4300 for moving the pressure piston 4220 in combination with the piston moving part 4300 and a linear moving motor 4400 connected to the piston moving part 4300 to make the piston moving part 4300 linearly reciprocate can do.

The linear motion motor 4400 can be connected to the piston moving part 4300 by the connecting bar 4500 and if the linear motion motor 4400 makes the piston moving part 4300 reciprocate linearly, The pressure piston 4220 coupled to the piston 4300 can reciprocate linearly.

The readout solution discharged from the hybridization storage portion 4100 may flow to the readout solution supply port 2800 through the flexible tube.

A plurality of types of genes may be mixed in the initial solution, and the gene discrimination apparatus of the present invention can perform reading of a plurality of kinds of genes.

The acidity or salinity of the initial solution can be adjusted to improve the accuracy of the gene reading, and the initial solution stored in the hybridization depressant can be additionally supplied with a control solution to adjust the acidity or salinity. A readout solution may be formed by mixing the initial solution with the control solution.

The hybridization module 4000 may include a third position sensor that senses a change in the position of the piston moving part 4300 and a pressure sensor that senses a pressure change inside the hybridization storage part 4100. The third position sensor and the pressure sensor can transmit the sensed information to the control unit 6000.

The pressure of the reading solution flowing into the first inlet of the lab-on-a-chip 1000 due to the pressure inside the hybridization storage unit 4100 can be calculated by calculating the pressure of the reading solution by the positional change of the piston moving part 4300 And the control unit 6000 can control the supply amount and the pressure of the reading solution.

The gene discrimination apparatus of the present invention comprises: a lab-on-a-chip 1000 formed by laminating a plurality of plate materials having different shapes, wherein a plurality of kinds of solutions are introduced, mixed, and discharged; An operation mechanism module 2000 including a tray 2200 for fixing the lab-on-a-chip 1000 to the upper surface thereof, and a gene reading unit 2100 for performing electrochemical reading on the gene introduced into the lab- ); A solution supply module 3000 located in the lower surface direction of the tray 2200 and having a plurality of scan portions and operating each of the plurality of scan portions to supply the solution to the lab-on-a-chip 1000; A hybridization module 4000 that generates a read solution to be read and transfers the read solution to the operation mechanism module 2000 so that a read solution is provided to the lab-on-a-chip 1000; A readout solution is supplied to the lab-on-a-chip 1000 and a solution stored in each of the plurality of scans is supplied to the lab-on-a-chip 1000 to perform an electrochemical readout on the genes in the readout solution. A control unit 6000 for controlling the solution supply module 3000 and the hybridization module 4000 and a display unit 5210 for displaying the results of the electrochemical reading performed by the gene reading unit 2100 on the screen, . ≪ / RTI >

The gene discrimination apparatus of the present invention includes a memory unit for storing the electrochemical readout result performed by the gene reading unit 2100 and a wireless communication unit for transmitting the electrochemical readout result performed by the gene reading unit 2100 to the external electronic device , ≪ / RTI >

The external electronic device may be any one or more of a smart phone, a tablet PC, a computer, and a video device.

The electrochemical readout result for the gene can be expressed in numerical or graphical form on the display unit 5210 and the matching information of the existing gene information stored in the control unit 6000 and the genetic information of the readout solution can be expressed.

When the USB device is inserted into the gene discrimination apparatus of the present invention, the information stored in the memory unit can be transmitted to the USB device.

In addition, the information stored in the memory unit is data-converted, and the genetically-read information of the data can be wirelessly transmitted to the electronic device through the wireless communication unit. The above-mentioned wireless information transmission can be performed in real time.

Hereinafter, a gene discrimination method using the gene discrimination apparatus of the present invention will be described.

In a first step, the lab-on-a-chip 1000 may be secured to the tray 2200. [

On this occasion, the lab-on-a-chip 1000 can be positioned so that the second inlet, the third inlet, and the outlet are facing the tray 2200.

In the second step, the cover portion 2300 of the operation mechanism module 2000 is brought into close contact with the tray 2200 so that the read-out solution supply port 2800 can be connected to the first inlet of the lab-

On the other hand, in the third step, the plurality of scanning units move so that the first scanning unit 3110 is connected to the second inlet of the lab-on-a-chip 1000, and the second scanning unit 3120 is connected to the second 3, and a discharge liquid storage portion 3130 may be connected to the discharge portion of the lab-on-a-chip 1000. [

In the fourth stage, the readout solution discharged from the hybridization module 4000 may flow to the first inlet through the readout solution supply port 2800. [

In the fifth step, the PCR solution can be performed by stopping the flow of the reading solution inside the lab-on-a-chip 1000 and providing the thermal energy from the heating unit 2400 provided in the operation mechanism module 2000. After completion of the polymerase chain reaction, the cooling unit 2500 can perform cooling for the lab-on-a-chip 1000.

In the sixth step, the read-out solution in which the fifth step has been performed may pass through the electrode portion of the lab-on-a-chip 1000 connected to the gene reading portion 2100, and electrochemical reading may be performed.

Here, the read solution may flow sequentially while being mixed with the washer liquid supplied from the first scanning unit 3110 and the buffer solution supplied from the second scanning unit 3120, and may flow to the electrode unit.

In the seventh step, the electrochemical readout performed by the gene reading unit 2100 can be displayed.

The fish species classification and transfer system may be provided, wherein the fish identified by the fish species is moved to a storage matched with the fish using the gene discrimination apparatus of the present invention.

The fish to be judged, which is the fish to be discriminated, can move to the discriminating section provided with the gene discriminating apparatus of the present invention by the conveyor belt. Then, the fish species of the fish to be judged can be discriminated by using the gene discrimination apparatus of the present invention, and the bar code can be attached to the fish to be judged. The fish to be judged to which the bar code is attached moves again to the classification section by the conveyor belt, and can be moved to and stored in a storage matching the fish to be judged in the classification section.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

1000: lab-on-a-chip
1100: channel portion 1110: channel plate
1111: Channel plate mixer hole 1112: Channel plate mixed channel
1113: valve inlet flow path 1114: valve discharge flow path
1115: Interruption interval 1116: Chamber
1117: Heat transfer prevention tread 1120: Top plate
1121: top plate mixer hole 1122: top plate hole
1122-a: first upper plate hole 1122-b: second upper plate hole
1122-c: third upper plate hole 1122-d: fourth upper plate hole
1123: first inlet portion 1124: second inlet portion
1125: third inlet portion 1126: outlet portion
1127: upper plate channel 1130: lower plate
1140: vortex space 1150: cap
1200: filter 1300: mixer
1310: Mixer plate 1311: Mixer plate hole
1320: Mixer cover 1400:
1410: valve plate 1411: valve plate hole
1411-a: first valve plate hole 1411-b: second valve plate hole
1420: valve cover 1500:
1510: Reading channel plate 1511:
1520: reading plate 1521: counter electrode
1522: reference electrode 1523: working electrode
2000: motion mechanism module
2100: gene reading unit 2110: detecting electrode unit
2120: Change measuring part 2200: Tray
2300: cover part 2400:
2500: Cooling section 2600:
2610: first pressure bar 2620: second pressure bar
2630: ball bearing 2800: reading solution supply port
3000: solution supply module
3110: first scanning section 3120: second scanning section
3130: discharge liquid storage part 3200: position adjustment part
3210: Support base 3220: Support base guide part
3230: Supporting rod guide 3231: First rack gear
3232: second rack gear 3233: positioning motor section
3240: Support part 3300:
3310: first pushing portion 3311: first pushing portion body
3320: second pushing portion 3321: second pushing portion body
3330: first feed screw 3340: second feed screw
3400: screw motor section
4000: Hybridization module
4100: hybridization storage section 4200: pressure cylinder section
4210: pressure cylinder 4220: pressure piston
4300: Piston moving part 4400: Linear moving motor
4500: Connection bar
5100: frame part 5110: frame floating board
5120: frame load plate 5130: frame column
5200: Case 5210:
5220: cover part case 5230: storage part
6000:

Claims (15)

A lab-on-a-chip in which a plurality of plate materials having different shapes are laminated and a plurality of kinds of solutions are introduced, mixed, and discharged;
An operation mechanism module including a tray for fixing the lab-on-a-chip to an upper surface thereof, and a gene reading unit for performing electrochemical reading of genes introduced into the lab-on-a-chip;
A solution supply module located in a direction of a lower surface of the tray and having a plurality of scans, and supplying solution to the lab-on-a-chip by operating each of the plurality of scans;
A hybridization module that generates a reading solution to be read and transfers the reading solution to the operation mechanism module so that the reading solution is provided to the lab-on-a-chip; And
The read solution is supplied to the lab-on-a-chip and a solution stored in each of the plurality of scans is supplied to the lab-on-a-chip to perform an electrochemical readout of the genes in the read solution, And a control unit for performing control on the supply module and the hybridization module,
The liquid supply module includes a first scanning part for supplying a washer liquid to the lab-on-a-chip, a second scanning part for supplying a buffer solution to the lab-on-a-chip, a discharge A position adjusting section for adjusting a position of the liquid storage section, the first scanning section, the second scanning section, and the discharge liquid storage section, and a pushing rod for selectively pushing and pushing the plunger of the first scanning section or the plunger of the second scanning section And a gene coding region.
The method according to claim 1,
The operation mechanism module includes:
A cover portion covering the tray,
A heating unit coupled to the cover unit and providing thermal energy to the lab-on-a-chip,
A cooling unit coupled to the cover unit and rotating the fan to cool the lab-on-a-chip,
A valve portion for pressing or releasing the valve portion provided in the lab-on-a-chip to control the flow of the read-out solution introduced into the lab-
And a read-out solution supply port for supplying the read-out solution transferred from the hybridization module to the lab-on-a-chip.
The method of claim 2,
The valve-
A first pressurizing rod that pressurizes or pressurizes a portion of the valve unit,
A second pressure bar for performing pressurization or pressurization to other portions of the valve portion, and
And a pressure operating part for selectively operating the first pressure bar or the second pressure bar.
The method according to claim 1,
The gene reading unit includes:
On chip, a detecting electrode portion contacting the electrode portion provided on the lab-on-a-chip to energize the solution flowing in the lab-
And a change measuring unit for measuring a change value of the current detected by the detection electrode unit and transmitting a change signal to the control unit.
delete The method according to claim 1,
The position adjustment unit,
A support for supporting the first scan unit, the second scan unit, and the discharge liquid storage unit,
A support base guide portion coupled to the support base and guiding the support base to move up and down,
And a support pedestal driving unit coupled to the support pedestal and providing power to move the support pedestal up and down.
The method according to claim 1,
Wherein the pusher-
A first pressing portion for pressing and moving the platen of the first scanning portion,
A second pressing portion for pressing and moving the platen of the second scanning portion,
A first conveying screw that passes through the body of the first pusher portion while being screwed and rotates to move the first pusher portion,
A second conveying screw which is screwed while passing through the body of the second presser portion and rotates to move the second presser portion,
And a screw motor unit for rotating the first conveying screw and the second conveying screw.
The method according to claim 1,
The hybridization module includes:
A hybridization storage unit in which one or more kinds of initial solutions containing genes are supplied and a control solution for controlling the acidity or salinity of the initial solution is supplied so that hybridization is performed,
A pressurizing cylinder portion having a pressurizing cylinder connected to the hybridization storing portion and pressurizing the readout solution in the pressurizing cylinder by movement of a pressurizing piston coupled with the pressurizing cylinder,
A piston moving in association with the pressure piston to move the pressure piston, and
And a linear motion motor connected to the piston to allow the piston to linearly reciprocate.
The method according to claim 1,
A bottom plate on which the operation mechanism module is installed on the upper surface, a frame load plate on which the solution supply module, the hybridization module, and the control unit are installed on the upper surface, And a frame part having a frame column which is coupled to the surface of the frame.
A lab-on-a-chip in which a plurality of plate materials having different shapes are laminated and a plurality of kinds of solutions are introduced, mixed, and discharged;
An operation mechanism module including a tray for fixing the lab-on-a-chip to an upper surface thereof, and a gene reading unit for performing electrochemical reading of genes introduced into the lab-on-a-chip;
A solution supply module located in a direction of a lower surface of the tray and having a plurality of scans, and supplying solution to the lab-on-a-chip by operating each of the plurality of scans;
A hybridization module that generates a reading solution to be read and transfers the reading solution to the operation mechanism module so that the reading solution is provided to the lab-on-a-chip;
The read solution is supplied to the lab-on-a-chip and a solution stored in each of the plurality of scans is supplied to the lab-on-a-chip to perform an electrochemical readout of the genes in the read solution, A control module for performing control on the supply module and the hybridization module, and
And a display unit for displaying the result of electrochemical reading performed by the gene reading unit on a screen,
The liquid supply module includes a first scanning part for supplying a washer liquid to the lab-on-a-chip, a second scanning part for supplying a buffer solution to the lab-on-a-chip, a discharge A position adjusting section for adjusting a position of the liquid storage section, the first scanning section, the second scanning section, and the discharge liquid storage section, and a pushing rod for selectively pushing and pushing the plunger of the first scanning section or the plunger of the second scanning section And a gene coding region.
The method of claim 10,
A memory unit for storing electrochemical readout results obtained by the gene reading unit, and
And a wireless communication unit for transmitting an electrochemical reading result of the gene reading unit to an external electronic device.
The method of claim 11,
Wherein the external electronic device is at least one of a smart phone, a tablet PC, a computer, and a video device.
A gene discrimination method using the gene discrimination apparatus according to claim 1,
i) securing the lab-on-a-chip to the tray;
ii) bringing the cover portion of the operating mechanism module into close contact with the tray so that a readout solution supply port is connected to the first inlet of the lab-on-a-chip;
on-the-fly chip, iii) the plurality of syringes move to connect the first scan portion to the second inlet of the lab-on-a-chip, the second scan portion to the third inlet of the lab- On-chip chip;
iv) flowing the readout solution discharged from the hybridization module through the readout solution supply port to the first inlet portion;
v) the reading solution is stopped from flowing inside the lab-on-a-chip, receiving thermal energy from the heat treatment provided in the operation mechanism module, and performing PCR;
vi) performing the electrochemical reading through the electrode portion of the lab-on-a-chip connected to the gene reading portion, wherein the reading solution in which the step v) is performed is performed; And
vii) displaying the result of electrochemical reading performed by the gene reading unit.
14. The method of claim 13,
Wherein in the step vi), the reading solution flows sequentially while mixing with the washer fluid supplied from the first scanning part and the buffer solution supplied from the second scanning part, and flows into the electrode part .
A fish species classification transfer system according to claim 1, wherein the fish identified by the fish species is moved to a storage matched with the fish using the gene discrimination apparatus of claim 1.

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