CN201051091Y - A chip lab including ultrasonic system - Google Patents
A chip lab including ultrasonic system Download PDFInfo
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- CN201051091Y CN201051091Y CNU2006201522705U CN200620152270U CN201051091Y CN 201051091 Y CN201051091 Y CN 201051091Y CN U2006201522705 U CNU2006201522705 U CN U2006201522705U CN 200620152270 U CN200620152270 U CN 200620152270U CN 201051091 Y CN201051091 Y CN 201051091Y
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Abstract
The utility model relates to a chip experiment chamber which at least comprises a sample area, a hybrid detection area, a supersonic source, and a signal detection and control device, wherein the sample area comprises at least a sample pool and a supersonic energy converter which is in direct contact with the sample pool and has the same number as the sample pool, the hybrid detection area comprises at least a hybrid detection pool in which one or multiple biological probe base materials complementing target molecules are placed, and at least a supersonic wave energy converter arranged on the upper surface or surrounding surface of the detection pool, and the supersonic energy converter can generate supersonic waves under the action of adjustable supersonic source. The chip experiment chamber has high detection accuracy and sensitivity.
Description
Technical field
The utility model relates generally to be used for chemistry and biochemical analysis, chemistry and bio-sensing, the synthetic and miniature instrument that detects, relates in particular to the chip lab that contains ultrasonic system that a kind of biology, chemistry and medicine are tested detection range.
Background technology
Laboratory operation processes such as traditional biology, chemistry and medicine comprise that specimen preparation, chemical/biological chemical conversion, sample fractionation, information signal detect and data processing.Wherein, usually need with each testing liquid by measure precision measure, proportioning and mixing, insert that (or in instrument) transforms or fractionation in one or more different physics or the chemical environment.This traditional lab analysis is the analytic process of very complicated, and obtain chemistry and biochemical information data needs expensive equipment, special laboratory and the technician who was subjected to specialized training.The characteristics of this lab analysis are that labour cost height, reagent consumption are big, reciprocal time long, the personal error probability is big, and even analysis cost height, analysis out of true and poor repeatability.
Closely during the last ten years, people constantly attempt, study a kind of height integrated form, integration microscale experiment chamber, promptly chip lab (Lab-on-chip) solves the problems referred to above, has obtained great success.This chip lab is to utilize semiconductor and micro-processing technology etc. to make functional units such as fluid channel, little valve, microreactor, microflow sensor, little detecting device on the long-pending small chip of face (body), constitute miniature biochemical system, full biochemistry/chemical process such as the pre-treatment of sample (sample), biology/chemical reaction, determinand separation, detection is integrated in realizes on the small chip, and rely on some detection methods such as electrochemical assay and optical detection, convert chemical signal to electric signal, machine or other signal processing apparatus make an explanation as calculated again.
Yet the detection effect of the chip lab of Chu Xianing it is still to fail very satisfied so far, and its defective is mainly reflected in that detection sensitivity is lower, accuracy of detection is still not too high.
Summary of the invention
The utility model proposes a kind of chip lab that contains ultrasonic system, this chip lab combines ultrasonic technology.The utility model purpose is: overcome the not high defective of detection effect, raising chip lab detection sensitivity and the degree of accuracy of chip lab of the prior art, enlarge the range of application of existing chip lab and enlarge its test sample kind.
The technical solution of the utility model is applicable to the chip lab (containing the biochip laboratory) of any kind of, is particularly useful for analyzing and detecting the chip lab of the bacterium with tough and tensile cell membrane.
Whole technical proposal of the present utility model is made up of following two parts, but be not limited to use simultaneously this two parts technology to same chip lab, using arbitrary part in this two parts technology all can be in the purpose that obtains to improve detection sensitivity and precision in varying degrees.
1) chain amplification region (PCR district) the sample area setting before at chip lab directly contacts the ultrasonic transducer of (need not liquid medium) with sample cell, this transducer can produce ultrasound wave under adjustable ultrasonic-frequency power supply effect, sample cell etc. or shear the wherein big molecule of target in the more effective broken lysate sample pond.Transducer is similar to sample cell contact position shape, can be various simple geometric shape, but preferably has the concave surface sphere of focusing function.
2) outer wall of the hybridization detection zone after the chain amplification region of chip lab (liquid flows into end and outflow end) is provided with the ultrasonic transducer of (need not liquid medium) that directly contact with this outer wall, this transducer can produce ultrasound wave under adjustable ultrasonic-frequency power supply effect, cleaning in the district after promoting hybridization reaction and quickening to strengthen hybridization reaction.Ultrasonic transducer also can be arranged on the inside surface of hybridization detection zone, but outside wall surface preferably, to avoid producing cross-infection etc.
The chip lab that contains ultrasonic system that the utility model relates to comprises sample area at least, hybridization detection zone and a ultrasonic sound source of cover and an input control device; Wherein, the sample area ultrasonic transducer that directly contacts with sample cell that comprises at least one sample cell, respective number.Also comprise: the cover plate of chip lab, sample flow into (or injection) runner, and runner also can design on the cover board to feed the sample cell cavity, base plate, pilot is fixed with the probe matrix of at least one probe, cleaning liquid (as water) inlet, sample liquid or cleaning fluid are discharged stomatodeum, sample cell cavity.
The related sample cell of the utility model is concave surface sphere, ellipse, cylindrical, conical or other rotation builds.
The related hybridization detection zone of the utility model comprises at least one can be built-in a kind of hybridization detection cell of fixing the bioprobe matrix of one or more and target molecule complementation, at least one is placed in hybridization detection cell top outside surface or the ultrasonic transducer on the outside surface all around.This ultrasonic transducer can produce ultrasound wave under adjustable ultrasonic-frequency power supply effect.
The related inner chamber with the contacted ultrasonic transducer of sample cell of the utility model has and sample cell appearance shape similar shapes, is concave surface sphere, ellipse, cylindrical, conical or other rotation builds; The inner chamber of related hybridization detection cell is rectangle, rectangle, rhombus, ellipse, cylindrical, conical or other rotation builds.
The related sample cell of the utility model and hybridization detection cell are integral type or the combined type made respectively.Their manufactured materials comprises macromolecular material, glass, quartz glass, pottery, metal and their any composition.
An embodiment of the present utility model is: the related chip lab that contains ultrasonic system comprises sample area, chain amplification region and hybridization detection zone.After sample liquid flows into the interior sample cell of (or being injected into) sample area, quilt such as sample cell is fully broken or/and after being sheared under the ultrasound wave effect, outside under the power drive, enter the chain amplification region by runner, there, sample liquid molecule (as the DNA that is separated out) is under different temperatures and reaction time, amplified repeatedly and increased chain, after reaching predetermined purpose, enter by runner in the cell cavity of hybridization detection cell, under ultrasound wave/sound field power effect that ultrasonic transducer produces, determinand in the sample liquid be fixed on matrix (probe) and go up molecule and actively hybridize, produce better hybridization reaction effect, thereby cause detection signal remarkable, detection sensitivity is enhanced.Behind the hybridization reaction, cleaning fluid enters from the filter washing water inlet, and under the ultrasound wave effect cell cavity internal memory thing is cleaned, afterwards through the discharge cell cavity of engaging in this profession.
Another embodiment of the present utility model is: the related chip lab that contains ultrasonic system can not contain the chain amplification region, only contains sample area and hybridization detection zone.So that quilt under the ultrasound wave effect such as the sample cell of some sample class is fully broken or/and after being sheared, directly under the power drive outside, enter by runner in the cell cavity of hybridization detection cell, there, under ultrasound wave/sound field power effect that ultrasonic transducer produces, determinand in the sample liquid be fixed on matrix (probe) and go up molecule and actively hybridize, produce better hybridization reaction effect, thereby cause detection signal remarkable, after detection sensitivity is enhanced hybridization reaction, cleaning fluid enters from the filter washing water inlet, and under the ultrasound wave effect cell cavity internal memory thing is cleaned, afterwards through the discharge cell cavity of engaging in this profession.
Description of drawings
Fig. 1 is the synoptic diagram of analysing and observe the cross section that contains of the related chip lab that contains ultrasonic system of the utility model.
Fig. 2 be the related chip lab that contains ultrasonic system of the utility model another embodiment (not containing the chain amplification region) contain the synoptic diagram of analysing and observe the cross section.
Embodiment
Below in conjunction with drawings and Examples the utility model is further specified.
As shown in the figure, the utility model embodiment comprises the cover plate (1) of this chip lab, sample flows into (or injection) runner (2), runner also can design on the cover board to feed sample cell cavity, sample cell (3), ultrasonic transducer (4), base plate (5), pilot (6,7), ultrasonic sound source and input control device (8), be fixed with the probe matrix (9) of at least one probe, hybridization detection cell (10), cleaning liquid (as water) inlet (11) (joining switch valve, not shown), sample liquid or cleaning fluid are discharged stomatodeum (12), ultrasonic transducer (13), pilot (14,15), sample cell cavity (20), sample area (100), chain amplification region (200) (specifically not illustrating), hybridization detection zone (300).
With reference to Fig. 1: after sample liquid flows into the sample cell cavity (20) of (or being injected into) sample cell (3) from the runner (2) of sample area (200), by predefined ultrasonic generation time and intensity etc., by comprising that ultrasonic sound source and input control device (8) are by lead (6) and (7) control ultrasonic transducers (4) work, after reaching the schedule time, quilt under the ultrasound wave effect such as the sample cell in the sample cell cavity (20) is fully broken or/and be sheared, then outside power (as the liquid pump, illustrate) drive down, be broken or/and the sample liquid that is sheared enters chain amplification region (200) by runner (20), there, sample liquid molecule (as the DNA that is separated out) is under different temperatures and reaction time, amplified repeatedly and increased chain, after reaching predetermined purpose, enter by runner (22) in the cell cavity (30) of hybridization detection cell (10), there, under the ultrasound wave/sound field power effect that produces by lead (14) and (15) control ultrasonic transducers (13) work by ultrasonic sound source and input control device (8), determinand in the sample liquid be fixed on matrix (9) and go up (probe) molecule and actively hybridize, produce better hybridization reaction effect, thereby cause detection signal remarkable, detection sensitivity is enhanced (the respective detection system is not shown).Behind the hybridization reaction, cleaning fluid enters from inlet (11), and under the ultrasound wave effect cell cavity (30) internal memory thing is cleaned, and discharges cell cavity (30) through engage in this profession (12) afterwards.
Another specific embodiment of the utility model is the chip lab that contains ultrasonic system that does not contain chain amplification region (200), and it is embodied as:
With reference to Fig. 2: after sample liquid flows into (or being injected into) sample cell (20) from the runner (2) of sample area (200), by predefined ultrasonic generation time and intensity etc., by comprising that ultrasonic sound source and input control device (8) are by lead (6) and (7) control ultrasonic transducers (4) work, after reaching the schedule time, quilt under the ultrasound wave effect such as the sample cell in the sample cell cavity (20) is fully broken or/and be sheared, then outside power (as the liquid pump, not shown) drive down, be broken or/and the sample liquid that is sheared enters in the cell cavity (30) of hybridization detection cell (10) by runner (22), there, under the ultrasound wave/sound field power effect that produces by lead (14) and (15) control ultrasonic transducers (13) work by ultrasonic sound source and input control device (8), determinand in the sample liquid be fixed on matrix (9) and go up (probe) molecule and actively hybridize, produce better hybridization reaction effect, thereby cause detection signal remarkable, detection sensitivity is enhanced (the respective detection system is not shown).Behind the hybridization reaction, cleaning fluid enters from inlet (11), and under the ultrasound wave effect cell cavity (30) internal memory thing is cleaned, and discharges cell cavity (30) through engage in this profession (12) afterwards.
Claims (9)
1. chip lab that contains ultrasonic system, it is characterized in that: this chip lab comprises sample area at least, hybridization detection zone and a ultrasonic sound source of cover and an input control device; Wherein, the sample area ultrasonic transducer that directly contacts with sample cell that comprises at least one sample cell, respective number.
2. a kind of chip lab that contains ultrasonic system according to claim 1 is characterized in that: described sample cell is concave surface sphere, ellipse, cylindrical, conical or other rotation builds.
3. a kind of chip lab that contains ultrasonic system according to claim 1 is characterized in that: described hybridization detection zone comprises at least one can be built-in a kind of hybridization detection cell of fixing the bioprobe matrix of one or more and target molecule complementation, at least one is placed in hybridization detection cell top outside surface or the ultrasonic transducer on the outside surface all around.
4. according to claim 1 or 3 described a kind of chip labs that contain ultrasonic system, it is characterized in that: described ultrasonic transducer can produce ultrasound wave under adjustable ultrasonic-frequency power supply effect.
5. a kind of chip lab that contains ultrasonic system according to claim 1, it is characterized in that: described have and sample cell appearance shape similar shapes with the inner chamber contacted ultrasonic transducer of sample cell, is concave surface sphere, ellipse, cylindrical, conical or other rotation builds.
6. a kind of chip lab that contains ultrasonic system according to claim 3 is characterized in that: the inner chamber of described hybridization detection cell is rectangle, rectangle, rhombus, ellipse, cylindrical, conical or other rotation builds.
7. a kind of chip lab that contains ultrasonic system according to claim 6 is characterized in that: hybridization detection cell and sample cell are the integral type or the combined type of manufacturing respectively.
8. according to the described a kind of chip lab that contains ultrasonic system of claim 1,2 or 7, it is characterized in that: the manufactured materials of sample cell comprises macromolecular material, glass, quartz glass, pottery, metal and their any composition.
9. a kind of chip lab that contains ultrasonic system according to claim 6 is characterized in that: the manufactured materials of hybridization detection cell comprises macromolecular material, glass, quartz glass, pottery, metal and their any composition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2006201522705U CN201051091Y (en) | 2006-12-31 | 2006-12-31 | A chip lab including ultrasonic system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2006201522705U CN201051091Y (en) | 2006-12-31 | 2006-12-31 | A chip lab including ultrasonic system |
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| Publication Number | Publication Date |
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| CN201051091Y true CN201051091Y (en) | 2008-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNU2006201522705U Expired - Fee Related CN201051091Y (en) | 2006-12-31 | 2006-12-31 | A chip lab including ultrasonic system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107362837A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | Be easy to disassemble applies the hypotype swine flu detection device of cheap material |
| CN107362838A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | The readily removable controller used in syphilis diagnosis micro fluidic device removed of liquid stream driving building blocks of function |
-
2006
- 2006-12-31 CN CNU2006201522705U patent/CN201051091Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107362837A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | Be easy to disassemble applies the hypotype swine flu detection device of cheap material |
| CN107362838A (en) * | 2016-05-13 | 2017-11-21 | 李榕生 | The readily removable controller used in syphilis diagnosis micro fluidic device removed of liquid stream driving building blocks of function |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080423 Termination date: 20121231 |