KR101938313B1 - Preparing method for undiluted solution of herb mixture and extracted liquor - Google Patents

Abstract

More particularly, the present invention relates to a method for producing leachate and a leach liquor for leaching mixed herbicidal extracts, and more particularly, to a leachate leachable leachate and leachate leach solution containing leachate, leavened timber, And (b) filtering the leach solution obtained in the step (a). The present invention also relates to a leach solution containing the leach solution prepared by the above method.
According to the present invention, it is possible to produce a leachate by immersing a mixed herb medicine containing a variety of active ingredients and having a useful pharmacological effect according to the present invention in a diluted alcohol to obtain an excellent It is possible to produce a mixed herbal leach with excellent functionality and functionality and further improve the sensibility and functionality by optimizing the content of the mixed herbal medicine, the leaching time, and the alcohol concentration of the diluted alcohol.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for preparing a crude herb extract,

More particularly, the present invention relates to a method for producing leachate and a leach liquor for leaching mixed herbicidal extracts, and more particularly, to a leachate leachable leachate and leachate leach solution containing leachate, leavened timber, And (b) filtering the leach solution obtained in the step (a). The present invention also relates to a leach solution containing the leach solution prepared by the above method.

Leachate is usually prepared by adding medicinal herbs such as ginseng or various fruits to the alcoholic beverages, etc., and aging. These leachers have unique herbs and fruit flavors, but they have the problem of causing headaches and hangover after drinking. In order to solve such a problem, leachate using functional vegetable has been developed. For example, Japanese Patent No. 10-0801195 discloses a method of producing a leach of Chunma using a cheongma, and Patent No. 10-1315406 discloses a method of producing corn beard Leachate and its production method are disclosed. Therefore, there is a continuing need for the development of leachate having both palatability and functionality using various functional vegetables.

On the other hand, wood senticosus (Eleutherococcus sessiliflorus ) is a deciduous broad-leaved shrub with a height of about 2m and has sharp thorns on branches. Leaves are short leaflets with five small leaves, and have long petiole. The flower is yellowish green, and when it is spring, it runs on the end of a long peduncle with an inflorescence. Each flower has 5 petals. The fruit is a ball with a diameter of 6-7㎜ and ripens black in September. It is distributed in various regions of the peninsula except for Gyeongnam and Chungnam. It is said that the shell of the root is edible. Leaves are divided into 5 small leaves, and they are also called acorns.

Leaflets (蘇葉, Perilla frutescens ), or foliage grasshopper, is a perennial plant belonging to the family Lamiaceae. It is a native of China and cultivated in the fields of various parts of Korea. It is also called Chazki or Chakugo. Its height is 20 to 80 centimeters. The whole is purple and fragrant, and the stem is square, straight and branches. Leaves are purple, with long petiole, broad ovate, sawtooth on edge, and hairs on both sides. Flowers are light purple, gunshot, corolla is tubular, lips, corolla and calyx.

Hunchback cordata ) is a perennial plant of Saururus chinensis. Leaves and stems are called fishy spots because they smell like fishy smell. It has various names such as Chinese herbal medicine, tea cup, Chinese medicine.

Achyranthes japonica NAKAI is a perennial plant with fleshy roots, also known as 牛,, 牛 莖, 골 骨 草, and 장 杖 根 root. Mogan stem grows straight up to a height of 1m with many branches. It has two petiole-shaped leaves facing each knot like a cow's knee and has short petiole. Leaves are elliptical, with sharp ends. Leaf length is about 10 ~ 15cm and there is no sawtooth on the edge and it is plain. Diuretic, penetration, and analgesic effects. The disease is known to be effective for the symptoms of poor urination, gonorrhea, hematuria, menopause, abdominal pain due to postpartum hemorrhage, pain in the knee, bruises.

Eucommia ulmoides OLIVER) is a plant called 仲), 사, 중), 사, 중), 사, 선 思 思,, 목, 棉 木 木 木, 棉棉,,, 석 선 석 석 석, 석 선 石,,, to be. Antihypertensive action, anti-aging, cholesterol lowering, anti-inflammation, sedation, pain relief, immune control, blood clotting, uterine contraction, antiallergic and antibacterial activity have been reported.

There have been cases in which the abovementioned phytopathogenic fungi, lobules, anilines, rice flies and mulberry trees have been used as herbal medicines, respectively.

DISCLOSURE OF THE INVENTION The present invention has been conceived in order to meet the needs of the prior art, and it is an object of the present invention to provide an ophthalmic solution, which has excellent functionality due to various active ingredients contained in mixed medicinal preparations comprising oak tree, lobule, And a process for producing a leached liquor.

Accordingly, it is an object of the present invention to provide a method for the production of (a) a process for the production of a plant, comprising the steps of: (a) dipping in a dilution alcoholic oak tree, lobules, And

(b) filtering the leach solution obtained in the step (a).

Another object of the present invention is to provide an infusible soju comprising a crude extract solution prepared according to the above method and an ophthalmic solution prepared by a method comprising the following steps:

(a) dissolving the whitening sugar in purified water to prepare a dissolution solution;

(b) immersing the dissolution liquid in an ophthalmic solution, an amniotic membrane or a lobule to effect leaching; And

(c) filtering the leach solution prepared in step (b).

In order to accomplish the object of the present invention, the present invention provides a method for producing a microorganism, comprising the steps of: (a) dipping in a dilution alcoholic oak tree, lobules, And

(b) filtering the leach solution obtained in the step (a).

In order to accomplish still another object of the present invention, there is provided an infusible leachate comprising a crude leach solution prepared according to the above method and an ophthalmic solution prepared by a method comprising the steps of: will be:

(a) dissolving the whitening sugar in purified water to prepare a dissolution solution;

(b) immersing the dissolution liquid in an ophthalmic solution, an amniotic membrane or a lobule to effect leaching; And

(c) filtering the leach solution prepared in step (b).

Hereinafter, the present invention will be described in more detail.

The present invention relates to a process for the production of (a) a step of dipping in a dilute alcoholic oak tree, a lobule, an egg plant, And

(b) filtering the leach solution obtained in the step (a).

(a) Leaching, by immersing in a diluting alcohol, an oak tree, lobules, anchovies,

In the present invention, the step (a) is a step of immersing five herbal medicine mixtures in a diluting alcoholic solution.

In the present invention, the oak tree may be at least one selected from the group consisting of leaves, branches, roots, and fruits. Preferably, the four oak trees are mixed at the same ratio.

In the present invention, the leaflets may be used for the leaflets, the whole plant may be used for the legs, the leaves and stems for the legs, and the bark for the legumes.

In the present invention, it is possible to include a step of drying for a predetermined time in order to remove water after washing the oak tree, lobules, pillows, knuckles and moss trees before the step (a).

That is, in the step (a) of the present invention, the oak tree, lobule, pill, knuckle and mugwort may be dried or free from moisture, preferably dried.

In the present invention, the diluted alcohol is an alcohol having an ethanol content of 30 to 50% (v / v), preferably 35 to 45% (v / v), most preferably 40 to 45% (v / v) It can be alcohol. A mixed herbal medicine is immersed in a dilution alcohol having the above range of ethanol concentration and leached to prepare a mixed herbal leavening stock solution properly containing a high quality aromatic component and having excellent functionality and containing a large amount of functional components such as phenols and flavonoids .

In the present invention, the diluted alcohol may be one obtained by mixing purified water with alcohol having an ethanol content of 95% and adding activated carbon. Preferably, the ethanol and the purified water having an ethanol content of 95% may be mixed in a volume ratio of 1: 1 to 1.5, and then the activated carbon may be added and left to stand for 1 to 5 days.

In the present invention, the leaching can be carried out at room temperature for 10 to 30 days, preferably 10 to 20 days. When leaching is carried out in the above range, there is a fear that the beneficial fragrance component will leak out, resulting in deterioration of the sensibility. In case of leaching under the above range, there is a problem that the desirable fragrance component and functional component are not sufficiently contained not.

In the present invention, the diluted alcohol is preferably used in an amount of 1 to 10% (w / v), 1 to 10% (w / v), 10 to 10% (w / v) / v) and 0.1 ~ 2% (w / v) of mulberry tree. Preferably 1 to 5% (w / v), 5 to 10% (w / v), 2 to 6% (w / v) 0.1 to 1% (w / v) of wood can be immersed to prepare a stock solution of mixed herb extract.

(b) filtering the leach solution obtained in the step (a)

The step (b) may be performed by filtering the crude herb extract solution obtained through step (a), wherein the filtration is preferably performed by putting a filter paper into a pressure filter and pressurizing the mixture at a pressure of 0.3 to 0.5 kgf / cm ² , But is not limited thereto and can be accomplished using filtration methods well known in the art. By carrying out the primary filtration in this way, it is possible to remove foreign matters and the like present in the leach solution.

The present invention also provides an infusible leachate comprising an extract of Echinosophora koreensis, an oil-in-water addition solution and a lyophilisation solution prepared according to the method comprising the following steps:

(a) dissolving the whitening sugar in purified water to prepare a dissolution solution;

(b) immersing an oak tree, an egg plant, or a leaflet in the solution, thereby leaching the plant; And

(c) filtering the leach solution prepared in step (b).

First, the production method of the above-mentioned alfalfa woody liquor, the weakly-milk-added liquid or the lyophilized-additive liquid will be described in detail.

(a) dissolving the whitening sugar in purified water to prepare a dissolution solution;

As the leaching solvent for preparing the additive liquid in the present invention, the above dissolution liquid is prepared by dissolving whitish white in purified water.

Concretely, the purified water is prepared by adding 0.2 to 1 kg of the whitening sugar to 1 L of the purified water. Preferably, the whitening sugar is added to the purified water at a ratio of 0.5 to 1 kg, and most preferably 0.6 to 0.9 kg, to 1 liter of purified water.

(b) immersing an oak tree, an egg plant, or a leaflet in the solution prepared in the step (a) and leaching the same;

In the step (b), the step (a) is carried out by immersing an oak tree, an egg plant, or a small leaf into the solution prepared in step (a).

The above-mentioned sites may be used for the acanthopanax japonica, the weevil millet or the lobules. The 1 L of the purified water contained in the solution may be dipped in a ratio of 0.2 to 1 kg, , It is possible to immerse at a rate of 0.5 to 1 kg, and most preferably 0.6 to 0.9 kg.

In the present invention, the leaching can be carried out at room temperature for 20 to 40 days, preferably 25 to 35 days.

(c) filtering the leach solution prepared in step (b);

The step (c) is a step of filtering each of the herbal solution was added through the step (b), the filtering is preferably a filter paper to be made to put the pressure filter to the pressure 0.3 ~ 0.5kgf / cm ² pressure However, the present invention is not limited thereto and can be carried out by using filtration methods well known in the art. By carrying out the primary filtration in this way, it is possible to remove foreign matters and the like present in the leach solution.

Thereafter, the leached liquor may be prepared by mixing the crude liquor detergent solution prepared according to the above-described method with the addition liquid of each herb medicine prepared by the above method.

In the present invention, the leavening stock may be characterized in that the stock solution for leaching, the solution of the solution of the oily substance, the solution of the solution of the weak alcohol, and the solution of the lobulated product are contained in a volume ratio of 1: 0.01-0.2: 0.01-0.2: 0.01-0.2.

The leached liquor of the present invention may further include various food additives in addition to the raw material for leaching and the liquid of addition of the crude drug, and the kind thereof is not limited. In one embodiment of the present invention, a jujube concentrate, glycine, refined salt, tartaric acid, and citric acid are added as the food additive to produce leachate.

In addition, the leach liquor of the present invention may be prepared by adding purified water to the leach solution after mixing the leach solution and the crude drug solution, so that the alcohol content of the final leachate can be controlled.

According to the present invention, it is possible to produce a leachate by immersing a mixed herb medicine containing a variety of active ingredients and having a useful pharmacological effect according to the present invention in a diluted alcohol to obtain an excellent It is possible to produce a mixed herbal leach with excellent functionality and functionality and further improve the sensibility and functionality by optimizing the content of the mixed herbal medicine, the leaching time, and the alcohol concentration of the diluted alcohol.

FIG. 1 is a graph showing the results of the electronic nasal chromatograms of four kinds of samples.
Fig. 2 is a graph showing the intensity of a selected peak in each sample.
3 is a view showing a perfume profile based on principal component analysis together with electronic nose analysis of each mainstream.
Fig. 4 is a diagram showing the results of electronic tongue analysis of each mainstream. Fig.

Hereinafter, the present invention will be described in detail with reference to preferred embodiments. Prior to this, terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms, and the inventor should appropriately interpret the concepts of the terms appropriately The present invention should be construed in accordance with the meaning and concept consistent with the technical idea of the present invention. Accordingly, it is to be understood that the constituent features of the embodiments described herein are merely the most preferred embodiments of the present invention, and are not intended to represent all of the inventive concepts of the present invention, so that various equivalents, And the like. Also, throughout the specification, when an element is referred to as "comprising ", it means that it can include other elements, not excluding other elements, unless specifically stated otherwise.

Hereinafter, the present invention will be described in detail.

< Example  1>

Mixed herbal medicine Of the undiluted solution  Produce

1) Step: Selection of herbal medicine

Oksigi trees, lobules, anchovies, kneecaps and mulberry trees suitable for food standards and specifications were purchased or collected, and only acceptable products were selected as raw materials except for defective products.

Leaf / branch / root / fruit were mixed in the same ratio. Leaf, leaf, stem, and bark were used as the raw material.

2) Step: washing, cutting and drying herbal medicine

The selected herbal raw materials were put in a washing machine, cleaned and then cut. After that, it was put in a dryer for drying for 36 hours to remove moisture of the raw material.

3) Step: Preparation of refined alcohol

200 g of ethanol of 95% (v / v) ethanol and 235 L of purified water were mixed, 200 g of activated carbon was added and the mixture was extracted for 48 hours to purify the alcohol. The final purified ethanol was 43% (v / v) ethanol.

4) Step: Leaching of mixed herbal medicine

The herbs washed and cut in step 2) were immersed in the purified water prepared in step 3) according to the composition shown in Table 1 for 14 days,

5) Step: Filtration

The herbal medicine was filtered out from the leach solution prepared in the step 4) to obtain a crude herb extract leach solution.

< Example  2>

Preparation of Fermentation Additives

1) Step: Selection of herbal medicine

Purchase or picking of oak tree, lobule and pill, which are in compliance with food standards and specifications. Only acceptable products excluding defective products were selected as raw materials.

Leaf / branches / roots / fruit were used for the oak tree, leaves were used for the leaves, and whole plants were used for the leaves.

2) Step: Preparation of solution

As shown in Table 2 below, white sugar was dissolved in purified water to dissolve.

3) Step: Leaching and fermentation

According to the composition shown in the following Table 2, the solution and the herb (raw) were put into each container and leached for 30 days:

4) Step: Filtration

The herbal medicine was sieved from the leach solution prepared in the step 3) to obtain each herbal fermented additive liquid.

< Example  3>

Mixed herbal medicine Leachate  Produce

The fermentation stock solution and additives prepared in Example 2 were blended in the amounts shown in Table 3 below, and 369 L of water was added to 410 L of the leavened stock solution prepared in Example 1 to prepare a mixed herb leach with 21% ethanol.

< Example  4>

Mixed herbal medicine Leachate Electronic nose  analysis

Electrical-nose analysis was conducted to objectively verify the flavor of the mixed herb leach produced in Example 3 above.

As a control, whiskey (comparative), Scotch blue, and mollusk were analyzed electronically with a mixed herb leach (strawberry yam) according to the present invention.

The headspace vial (22.5 x 75 mm, PTFE / silicon septum, aluminum cap) was added to the mixed herb leachate and 1 g of the control sample and the mixture was stirred at 70 rpm at 500 rpm for 20 minutes. (2m X 0.18mm DB5 / DB1701) and two flame ionization detectors (FIDs) were used for the analysis. ). The oven temperature was increased from 40 ° C to 250 ° C by 3 ° C per second and maintained at 250 ° C for 5 seconds. The flow rate of hydrogen gas was 1mL / min. The components of the separated peaks were estimated using AcoChembase (Alpha MOS) based on the Kovats index library, and the results of four replicates were used for principal component analysis.

The results are shown in FIG. 1 to FIG.

The results of comparing chromatograms of four kinds of liqueurs are shown in Fig. In order to compare the perfume patterns between the samples, the peak (Fig. 2) showing significant differences between samples in DB-5 was selected and analyzed by principal component analysis (Fig. 2) PCA).

As shown in FIG. 3, the data of the first principal component (PC1) was 99.09% of the data deviation and the data of the second principal component (PC2) was 0.55% of the total data deviation, respectively, as a result of PCA The total explanatory power was 99.64%.

The comparative samples (1), (2) and Scotch blue (3) were classified according to the yellowness pattern (4) and the PC1 pattern.

< Example  5>

Mixed herbal medicine Leachate Electronic tongue  analysis

The four samples used in the experiment in Example 4 were diluted 1/3 times with distilled water and filtered to use for the analysis of the electronic tongue.

The instrument used was ASTREE electronic tongue (Alpha MOS, Toulouse, France) and analyzed the sample's taste pattern by analyzing the components eluted in the liquid matrix. Each of the five sensors had five specific flavors (SRS, It is mainly involved in one of STS (salty taste), UMS (rich taste), SWS (sweet taste), BRS (bitter taste) representative).

Experimental methods for electronic tongue analysis are shown in Table 4 below:

The results are shown in FIG. 4 and Table 5 below.

The results of comparing the overall patterns of each taste sensor for the four main alcoholic tongue analyzes are shown in Table 4 and Table 5 below:

Claims (7)

(w / v), 1 ~ 10% (w / v), 1 ~ 10% (w / v) and 1 ~ 10% (w / v) and 0.1-2% (w / v) of Paralichthus edodes in the diluted alcohol; And
(b) filtering the leach solution obtained in the step (a). delete The method of claim 1, wherein the leaching is performed at room temperature for 10 to 30 days. The method of claim 1, wherein the diluted alcohol is a alcohol having an ethanol content of 30 to 50% (v / v). A leachate comprising a crude extract solution prepared according to the method of any one of claims 1, 3, and 4 and an extract of Ogaki bark,
(a) dissolving the whitening sugar in purified water to prepare a dissolution solution;
(b) immersing an oak tree, an egg plant, or a leaflet in the solution, thereby leaching the plant; And
(c) filtering the leach solution prepared in step (b). The leachate according to claim 5, wherein the leaching of step (b) is carried out at room temperature for 20 to 40 days. [6] The leachate according to claim 5, wherein the stock solution for leaching, the solution for the addition of the acetic acid, the solution for the weak acid solution and the solution for the lyophilization are contained in a volume ratio of 1: 0.01-0.2: 0.01-0.2: 0.01-0.2.

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