KR101915136B1 - Compositions for preventing or treating andropause-related symptoms - Google Patents

Abstract

The present invention relates to a composition for preventing or alleviating andropause-related symptoms, wherein the composition may include a black garlic extract and a Lepidium meyenii extract as active ingredients. According to the composition for alleviating the andropause-related symptoms of the present invention, it is possible to prevent and alleviate the andropause-related symptoms while containing natural extracts as the active ingredients.

Description

Technical Field [0001] The present invention relates to compositions for the prevention and improvement of menopausal symptoms syndrome,

The present invention relates to a composition for preventing and improving male menopausal symptoms. More particularly, the present invention relates to a composition for preventing and / or ameliorating menopausal symptoms in men, which comprises black germ extract, maca extract, olive extract and Krachaidam extract as an active ingredient and has an effect of preventing or ameliorating symptoms according to menopausal symptoms .

Menopause is a syndrome caused by endocrine or mental physical changes in old men and women, and is a characteristic syndrome of women and men according to changes in hormones and the like.

Men's menopause, in particular, is a process in which men experience a general decline in physical and mental aspects as the body ages. Men have been referred to as menopausal women for symptoms such as nervousness, emotional distress, depression, dizziness, facial flushing, sweating, sleep disturbances, decreased vitality, decreased memory, decreased work capacity, and decreased sexual desire (Werner AA. J Am Med Assoc 1939; 112: 1441-1443). Men's menopause means that men's characteristics, overall vitality and mood are gradually deteriorated due to the deterioration of male hormones. As with women, men also begin to develop menopausal symptoms from around the age of 50. As the age increases, the incidence of adrenal gland function, sperm, fertility, Leydig cell function declines and serum testosterone decreases with age Menopausal symptoms will appear. According to ISSAM (International Society for the Study of the Aging Male), depression of male hormone leads to a decrease in sexual desire, a decrease in erectile function, a decrease in mood accompanied by depression and irritation, a decrease in memory, And skin aging, decreased bone density, and increased visceral fat (The Korean Academy of Sexual Surgery. In addition to other environmental factors, male hormone replacement therapy has been known as the main therapy for the menopausal decline of male hormones. Male hormone replacement therapy includes eating or applying drugs, sticking patches, and injecting therapy.

Symptoms caused by a decrease in male hormones in more than 50 males are reported worldwide, with a reported rate of about 0.5%. Examples of such symptoms include sexual desire, decreased foot function, increased fatigue, decreased vitality, decreased muscle mass and increased body fat, depression, and male osteoporosis. Male hormone replacement therapy is used as a countermeasure against the above symptoms, or a health food containing drugs or other natural substances for improving erectile dysfunction symptoms is used.

However, the treatment of male menopausal disease through male hormone therapy increases the size of the prostate gland to increase the size of the prostate cancer that has already occurred, or the side effects such as worsening of sleep apnea, gynecomastia, erythropoiesis, and increased risk of cardiovascular disease You can follow.

Therefore, a composition for alleviating or improving male menopausal period using natural products has been studied, and a combination prescription of natural products that can achieve a certain range of effects without side effects has been spotlighted. In addition, by using a composite composition of various natural extracts, various natural products and mixtures that have higher effects with less content and no side effects have been actively studied.

KR 10-2017-0054682 A

It is an object of the present invention to provide a composition for preventing and ameliorating male menopausal syndrome, which comprises a natural extract as an active ingredient and has an effect of preventing and improving male menopausal symptoms.

In addition, the composition for prevention and improvement of male menopausal period syndrome includes natural extract as an active ingredient so as to have a remarkably excellent effect for prevention and improvement of menopausal period without side effects.

Another object of the present invention is to provide a functional food composition using the composition for preventing and improving menopause.

In order to achieve the above object, the composition for preventing and improving male menopausal symptoms according to one embodiment of the present invention comprises black garlic extract and maca extract as an active ingredient.

The composition for preventing and improving male menopausal symptoms may further comprise an olive extract.

The composition for preventing and improving male menopausal symptoms may further comprise Krachaidam extract.

The composition for preventing and / or ameliorating male menopausal symptoms may further comprise any one selected from the group consisting of peony extract, jelly extract, sugar beet tree extract, Namchun extract, and mixtures thereof.

The composition for preventing and improving male menopausal symptoms may further comprise a fennel extract.

The pharmaceutical composition according to another embodiment of the present invention may include a composition for preventing and improving male menopausal symptoms.

The food composition according to another embodiment of the present invention may include a composition for preventing and improving male menopausal syndrome.

Hereinafter, the present invention will be described in more detail.

The composition for preventing and improving male menopausal symptoms according to one embodiment of the present invention includes black garlic extract and maca extract as an active ingredient.

The male menopausal syndrome of the present invention refers to symptoms accompanied by symptoms such as depression, nervousness, fatigue, muscle weakness, memory or concentration deterioration, osteoporosis and sexual dysfunction due to a gradual decrease in male hormone. In 2004, In addition to 'late-onset hypogonadism (LOH)' as determined at the International Society for the Study of Aging Men, 'age-associated testosterone deciency syndrome (TDS)' it means. Men's Menopausal Syndrome is a clinical and biochemical syndrome associated with typical symptoms and serum testosterone deficiency experienced in men with increasing age, which can impair quality of life and negatively affect the function of various organs .

In the present invention, the term "garlic" refers to garlic which is blackened through browning by aging at a high temperature of garlic (white garlic). The black color of black garlic, which is aged at high temperature, can be obtained by heat treatment at a high temperature during processing, such as amino acid peptide, caramel reaction, non-enzymatic browning reaction by reaction of α-amino group of protein with sugar, Lt; RTI ID = 0.0 > non-enzymatic browning of < / RTI > In addition, the organic matter of garlic is decomposed during the ripening process, and the smell of garlic is reduced. The term "black garlic" as used in the present invention includes all of garlic showing a browning phenomenon through high-temperature aging processing, and its production method is defined to include all those processed in a conventional manner in the field.

The term maca (Lepidium meyenii) referred to in the present invention refers to a cruciferous biennial plant originating in the Peruvian Andes. The maca is the only one of its kind in the daddy cranium, which has a fleshy hypocotyl, coupled with a straight root to form the body. The size and shape of the macaques varies, and the defecation has gold, white, red, purple, blue, black, green, etc. The seeds of the mother plant have the same roots as the mother tree, . White roots are also the most popular, and the size and sweetness of white roots is popular in Peru.

When the black garlic extract and the maca extract are mixed, the effect of preventing and improving male menopausal symptoms can be enhanced by the synergistic effect. In particular, it has a higher synergistic effect than the case of using the black garlic extract or the maca extract alone, so that it can exert a high effect with a smaller amount and has no side effect problem.

As used herein, the term " extract " means that it is used in the art as a crude extract as described above, but broadly includes fractions obtained by further fractionating the extract. That is, the maca extract includes not only those obtained by using the above-described extraction solvent but also those obtained by further applying a purification process thereto. For example, a fraction obtained by passing the above extract through an ultrafiltration membrane having a constant molecular weight cut-off value, and a separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) The fraction obtained by the purification method is also included in the maca extract of the present invention.

The extract used in the present invention can be prepared in a powder state by an additional process such as vacuum distillation and freeze-drying or spray drying.

As used herein, the term " comprising as an active ingredient " is meant to include an amount sufficient to achieve efficacy or activity of the following maca extract. Since the present invention is a composition extracted from maca, which is a natural plant material, there is no adverse effect on the human body even when it is administered in an excessive amount. Thus, the quantitative upper limit of the amount of maca extract contained in the composition of the present invention can be selected by a person skilled in the art within a suitable range.

The composition for preventing and improving male menopausal symptoms may further comprise an olive extract.

The olive in the present invention refers to the fruit of an olive tree (olea europaea). Olives are commonly used in Italian and other Mediterranean watersheds and play a very important role in their food culture. Although raw olives have a bitter taste, bitterness disappeared in water, salt water or alkaline solution for a certain period of time, and the original flavor is renewed and the texture is softened.

When the black garlic extract and maca extract further include the olive extract, there is an advantage that the effective ingredient for preventing and improving male menopausal syndrome increases and the improvement effect of male menopausal syndrome is greatly increased.

The composition for preventing and improving male menopausal symptoms may further comprise Krachaidam extract.

The traditional herbal medicine produced in Thailand, Laos, etc., which is called Krachaidam or King hammer in the present invention, is Kaempferia parviflora.

In the case of the black garlic extract, the maca extract and the olive extract, the active ingredient for preventing and ameliorating male menopausal syndrome is increased and the improvement effect of male menopausal syndrome is greatly enhanced when the extract contains the above-mentioned extract.

The composition for preventing and / or ameliorating male menopausal symptoms may further comprise any one selected from the group consisting of peony extract, jelly extract, sugar beet tree extract, Namchun extract, and mixtures thereof.

The peony (Paeonia suffruticosa) is also called Mokan (牧 丹), and its height is about 2m, and its branches are thick and hairless. Leaves are three-ply, small leaves are ovate, split into 2 to 5, leaf surface has no hairs, backside has fine hairs and often white.

Clematis mandshurica Ruprecht is a deciduous plant that grows in the mountains and fields of each country. Growth environment grows in high soil fertility and high soil fertility.

Berberis poiretii, also known as Tangkorubi, grows in the mountains and branches with thin, hairs-free branches, a slightly dull line, purplish brown, and a height of 2m. Visibility is simple, or it is divided into 3 pieces and is 0.5 ~ 1cm in length. Leaves are alternate in young branches and are short-lived, and they are 2 ~ 4cm long in inverted shape. The front side of the leaf is green, the back side is gray green, and the edge has no saw tooth.

The Namcheon (Nandina domestica) is also called Namcheon-gu, Namcheon-myeon, and is native to China. It is planted in the gardens in the southern part and bonsai in the northern part. It grows about 3m in height. Several trees grow from the bottom, but the wood is yellow.

When the peony extract, the jujube extract, the sugar beet tree extract, the Namcheon extract, or a mixture thereof is used, the effect of improving the male menopausal syndrome is enhanced, so that it has the advantage of achieving a higher effect with a smaller dose.

Preferably, the composition for preventing and ameliorating male menopausal period syndrome may further comprise peony extract, jelly extract, sugar beet tree extract and Namchun extract.

When the peony extract, the jelly extract, the sugar beet tree extract and the Namcheon extract are included, the synergistic effect of mixing may be high.

More preferably, the composition for preventing and / or ameliorating male menopausal symptoms comprises 10 to 70 parts by weight of the peony extract, 100 parts by weight of the maca extract, 10 to 70 parts by weight of the above-mentioned udder extract; 10 to 70 parts by weight of the sugar beet extract and 10 to 70 parts by weight of the Namchun extract.

According to the above range, the synergistic effect of the combination of peony extract, jujube extract, sugar beet tree extract and Namchun extract promotes the secretion of testosterone, thereby greatly enhancing the effect of preventing and improving male menopausal period.

The composition for preventing and improving male menopausal symptoms may further comprise a fennel extract.

The fennel (Foeniculum vulgare) comes from the Latin foenum, which means 'hay', the name which originates from the peculiar hay smell of the fennel. The height is 1.5 to 2 m. The stem grows straight and the genus is empty. Leaves are long, sheath-like, pointed end, thin like 3 ~ 4 like feathers.

When the fennel extract is included, the synergistic effect of mixing can exert a synergistic effect on male menopausal symptoms compared with the single extract.

The pharmaceutical composition according to another embodiment of the present invention may include a composition for preventing and improving male menopausal symptoms.

The food composition according to another embodiment of the present invention may include a composition for preventing and improving male menopausal syndrome.

The preferred dosage of the extract of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the administration route and the period of time, but can be appropriately selected by those skilled in the art. However, for the desired effect, the extract is preferably administered at a dose of 0.01 mg / kg to 10 g / kg per day, preferably 1 mg / kg to 1 g / kg per day. The administration may be carried out once a day or divided into several doses. Therefore, the dose is not intended to limit the scope of the present invention in any aspect.

&Quot; Health functional food "as defined herein means food prepared and processed using raw materials or ingredients having functionality useful to the human body in accordance with Law No. 6727 on Health Functional Foods." Functional " Structure and function of the nutrient to control or physiological effects, such as to obtain a beneficial effect for health is intended to eat.

When the extract according to the present invention is used as a food additive, the extract may be directly added or used together with other food or food ingredients, and may be suitably used according to a conventional method. Examples of the food to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

According to the composition for improving menopausal age of the present invention, it is possible to prevent and ameliorate male menopausal syndrome while containing natural extract as an active ingredient.

In addition, the composition for prevention and improvement of male menopausal syndrome includes a natural extract as an active ingredient, and can exert a considerably superior effect for prevention and improvement of male menopausal age without side effects.

The composition for preventing and ameliorating male menopausal period syndrome may be used as a food composition or the like to provide various functional foods, beverages and the like.

Figure 1 relates to the results of cytotoxicity experiments for the examples according to the present invention.
FIG. 2 is a graph showing the test results of increasing the secretion of testosterone in TM3 cells according to the example of the present invention.
FIG. 3 relates to an experiment for increasing testosterone secretion in an oxidative stress environment in TM3 cells according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

[ Manufacturing example : Preparation of extract]

One. Black garlic  Preparation of extract

The extract was subjected to hot water extraction at 100 ° C for 4 hours in a volume ratio of 20 times to the black garlic, and the extract was filtered with a filter paper and dried under reduced pressure to prepare a black garlic extract (A).

2. Preparation of other extracts

(D), peony extract (E), jelly extract (F), sugar beet tree extract (G), and sugar extract (B), olive extract (C) Namchun extract (H) and fennel extract (I) were prepared.

3, Preparation of extract mixture

The extracts of black garlic extract (A), maca extract (B), olive extract (C), Krachaidam extract (D), peony extract (E), jelly extract (F) ), Namchun extract (H) and fennel extract (I) were mixed at the same ratios as in the following Table 1 to prepare Examples X1 to X14.

A B C D E F G H I X1 10 10 X2 10 10 10 X3 10 10 10 10 X4 10 10 10 10 10 X5 10 10 10 10 10 X6 10 10 10 10 10 X7 10 10 10 10 10 X8 10 10 10 10 One One One One X9 10 10 10 10 2 2 2 2 X10 10 10 10 10 3 3 3 3 X11 10 10 10 10 5 5 5 5 X12 10 10 10 10 7 7 7 7 X13 10 10 10 10 10 10 10 10 X14 10 10 10 10 5 5 5 5 10

(Unit: parts by weight)

[ Experimental Example: Individual  The extract and Example  Cytotoxicity confirmation]

1. Identification of cytotoxicity

The cytotoxicity test for the individual extract and extract mixture of the present invention was performed. The cytotoxicity of the individual extracts and mixed extracts was measured in a TM3 cell line derived from rat testis and a ladig cell that secretes testosterone, a male hormone. TM3 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin / streptomycin at 37 ° C and 5% CO2.

TM3 cells were cultured in a 96-well plate at a concentration of 1 × 10 ⁵ cells / ml at 37 ° C and 5% CO ₂ . Each of the individual extracts of A to I and the mixture of extracts of X1 to X14 were treated at a concentration of 20 占 퐂 / ml and cultured for 24 hours.

MTT solution was added. After 3 hours, the supernatant was removed. 100 μl of DMSO was added to each well. Absorbance was measured at 540 nm using an ELISA reader. The results are shown in FIG. Referring to FIG. 1, it can be confirmed that the extract of the individual extract and the mixture of extracts did not show cytotoxicity against TM3 cells at a concentration of 20 μg / ml.

2. Experimental study on testosterone increase effect of individual extract and extract mixture

TM3 cells were cultured in DMEM supplemented with 10% FBS at 37 ° C and 5% CO ₂ at a concentration of 1 × 10 ⁵ cells / ml in a 24-well plate. Each of the individual extracts of A to I and the mixture of extracts of X1 to X14 were treated at a concentration of 20 占 퐂 / ml and cultured for 24 hours. Testosterone ELISA kit (ADI-900-065, ENZO Life Sciences, USA) was used to measure the testosterone content. The results are shown in FIG. As a control, TM3 cells cultured under the same conditions without any addition were used.

As a result, referring to FIG. 2, it can be seen that the mixture of the extract according to the present invention promotes the secretion of testosterone from TM3 cells by the synergistic effect upon mixing, as compared with each individual extract. X1 to X3, a synergistic effect appears.

On the other hand, when X4 to X7 are referred to, it is more advantageous to mix them in a certain mixing range, such as X8 to X12, rather than mixing peony extract, jujube extract, sugar beet tree extract and Namchun extract alone. It can be seen that it can be put out.

On the other hand, it can be seen that X 14 is most effective in increasing testosterone secretion of TM3 cells.

3. Experimental study on increase of testosterone in individual extracts and extract mixture under hydrogen peroxide induction condition

TM3 cells were cultured in DMEM supplemented with 10% FBS at 37 ° C and 5% CO ₂ at a concentration of 1 × 10 ⁵ cells / ml in a 24-well plate. The individual extracts of A to I and the extract mixture of X1 to X14 were treated in each well at a concentration of 20 占 퐂 / ml and treated with H ₂ O ₂ at a concentration of 100 μM for 24 hours. Testosterone ELISA kit (ADI-900-065, ENZO Life Sciences, USA) was used to measure the testosterone content. The results are shown in FIG.

Referring to FIG. 3, it can be seen that testosterone secretion is promoted in an oxidative stress environment induced by hydrogen peroxide when the extract mixture of the present invention is used. In particular, it can be seen that not only the synergistic effect of X1 to X4 is high but also the effect of X3 and X4 is as much as or better than the normal control group which does not treat hydrogen peroxide.

On the other hand, in the case of X5 to X7, the synergistic effect according to the mixing use is lowered in the oxidative stress environment induced by hydrogen peroxide. However, in the case of X8 to X12 and X14 according to mixing use, even in the oxidative stress environment induced by hydrogen peroxide, It can be seen that the synergistic effect is largely maintained.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (7)

Black garlic extract;
Maca extract and
Containing an olive extract as an active ingredient
A composition for preventing and improving male menopausal symptoms. delete delete delete delete A pharmaceutical composition for preventing and improving male menopausal symptoms comprising the composition according to claim 1. A food composition comprising the composition for preventing and improving male menopausal symptoms according to claim 1.

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