KR101906766B1 - Composition comprising an extract of Artemisia lvandulaefolial or Aster scaberl for treating insect disease - Google Patents

Abstract

TECHNICAL FIELD The present invention relates to a composition for treating diseases caused by insects, which comprises an extract of Chrysanthemums as an active ingredient. The chrysanthemum extract according to the present invention is useful for the treatment of bee diseases such as Nogema spores and amoebas because it has an effect of inhibiting Nogema spores or amoeba cysts. In addition, the chrysanthemum extract of the present invention has no toxicity as a natural substance and can be used as a therapeutic agent for bee disease, thereby improving the productivity of honey and improving the fertility of plants.

Description

[0001] The present invention relates to a composition for treating diseases caused by insects, which comprises an extract of Artemisia sp.

TECHNICAL FIELD The present invention relates to a composition for treating diseases caused by insects, which comprises an extract of Chrysanthemums as an active ingredient.

Bees are one of the oldest insects that survived without evolution, and are group insects. These bees have used honey, pollen, royal jelly, and propolis from nature to protect them from breeding and external environment. Bees used by humans provide humans with honey, and humans prepare specimens that dilute 1: 1 sugar and water to provide them as food for bees. However, while the honey contains various micronutrients and vitamins, the specimen does not contain any nutrients other than sugar, which causes the bees to lose their immunity, which is a bad influence on honey production and pollen activity .

The scientists' claim that 'humans can not eventually survive if bees can not live' is not the story of yesterday. In Korea, for example, there is a widespread damage to the farmhouse due to the presence of bee blooms all over Korea, including the Gyeongnam area and the Gyungchang area. In the past, the disease of bees in Korea was caused by the American buzzers that rotten about, the European buzzer bottle, the plaster of the bee larvae sprinkled with lime, the hardened plaster bottle, the neze, the paralysis bottle and the amoeba. However, most of them were treated simply when they were given medicines purchased through an amicel.

However, recent honey bee disease is a serious disease, such as a heavy beetle which is known to be strong in some species or diseases. Although the treatment of a serious honey disease by introducing a therapeutic agent by various chemical synthesis has been introduced, it has not been solved fundamentally but it has been gradually spreading, and research is needed to effectively treat the disease of honey bee.

Korean Patent Publication No. 2014-0017291 Japanese Patent No. 5186254

Accordingly, the inventors of the present invention have confirmed that an excellent effect is obtained in the treatment of Nezema or amoebiasis, which is an insect-induced disease, in the flavor extract.

Accordingly, an object of the present invention is to provide a pharmaceutical composition for preventing and treating a bee's nosema disease or amoeba disease, which contains an Aster scaber extract as an active ingredient.

Another object of the present invention is to provide a bee-growing feed for the prevention and treatment of bee's nosema disease or amoeba disease containing an Aster scaber extract as an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing and treating a bee's nosema disease or amoeba disease, which contains an Aster scaber extract as an active ingredient .

In one embodiment of the present invention, the flavor extract may be extracted with a solvent selected from the group consisting of water, methanol, ethanol, hexane, butanol, ethyl acetate, chloroform and a mixed solvent thereof.

In one embodiment of the present invention, the composition may inhibit spores of the Nogema's disease or cysts of the amoeba disease.

The present invention also provides bee-fed diets for the prevention and treatment of bee's nosema disease or amoeba disease containing an Aster scaber extract as an active ingredient.

The chrysanthemum extract according to the present invention is useful for the treatment of bee diseases such as Nogema spores and amoebas because it has an effect of inhibiting Nogema spores or amoeba cysts. In addition, the chrysanthemum extract of the present invention has no toxicity as a natural substance and can be used as a therapeutic agent for bee disease, thereby improving the productivity of honey and improving the fertility of plants.

Fig. 1 shows a photograph of a Nogema spore (microscope 400 magnification, red arrow) isolated from a midgut of a bee.
Fig. 2 shows photographs of Nogema spores infected with IPL-L65Y (microscope 400 magnification, red arrow).
Fig. 3 shows photographs of amoeba cyst (microscope 400 magnification, red arrow) infected with amoeba cyst and IPL-L65Y isolated from bee's midium.
Fig. 4 is a graph showing the anti-Najema effect of the colostrum extract.
FIG. 5 is a graph showing the effect of anti-Nemema on the level of the gene (mRNA) of the colostrum extract.
6 is a graph showing the anti-amoeba effect of the marine extract.

The present invention is characterized by providing a composition for preventing and treating bee's nosema disease or amoeba disease, which contains an Aster scaber extract as an active ingredient.

The ' Aster scaber l' of the present invention is a perennial plant of Asteraceae and is known to be effective for bruising, enteritis, poisoning, blood circulation and inflammation.

It is known that the above-mentioned functions are present in the conventional anesthesia, but it is not known that the insect-induced diseases can be prevented or treated.

Accordingly, the present invention was firstly confirmed that insect-induced diseases can be prevented or cured by confirming the effect of suppressing spore or amoeba cysts of Nogema's disease in the flavor extracts.

Accordingly, the present invention can provide a composition for preventing and treating bee's nosema disease or amoeba disease, which contains an Aster scaber extract as an active ingredient.

The chrysanthemum extract according to the present invention can be obtained by extracting and separating from nature using an extraction and separation method known in the art, and the 'extract' defined in the present invention can be extracted from an animal And includes, for example, crude extracts of azaleas, polar solvent-soluble extracts, and non-polar solvent-soluble extracts.

As an appropriate solvent for extracting the extract from the asteraceae, any pharmaceutically acceptable organic solvent may be used, and water or an organic solvent may be used. Examples of the solvent include, but are not limited to, purified water, methanol acetone, ether, benzene, chloroform (such as methanol, ethanol, propanol, isopropanol, and butanol) chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane may be used alone or in combination.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the method for producing the fragrance extract of the present invention, and any known method can be used.

For example, the flavor extract contained in the composition of the present invention can be prepared into a powdery state by an additional process such as vacuum distillation, freeze-drying, or spray-drying, with the primary extract extracted by the above hot water extraction or solvent extraction method . Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, the fragrance extract is a concept including all the extract, fraction and purified product obtained in each step of extraction, fractionation or purification, a diluted solution thereof, a concentrate or a dried product.

In addition, the present invention can provide a beekeeping feed for the prevention and treatment of bee's nosema disease or amoeba disease containing an Aster scaber extract as an active ingredient.

Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are intended to further illustrate the present invention, and the scope of the present invention is not limited to these examples.

≪ Example 1 >

How to Separate Spore

The midgut (medium) was isolated from the live bee infected with Nogema and then pulverized with a homogenizer. The mixture was suspended in PBS and centrifuged at 1,000 rpm for 10 minutes to remove large foreign particles. The supernatant was collected And centrifuged at 3,000 rpm for 10 minutes. The precipitated Nemema was collected, suspended in PBS, and counted.

≪ Example 2 >

How to separate amebic cysts

After the live bees infected with amoebic cysts were stunned, the medium was isolated, pulverized with a homogenizer, suspended in PBS, centrifuged at 1,000 rpm for 10 minutes to remove large particles, and the supernatant was recovered After centrifuging at 3,000 rpm for 10 minutes, the precipitated amoeba was recovered and suspended in PBS to count the number of cysts.

≪ Example 3 >

Anger  Extract Antioxidant  effect

<3-1> Anger  Method of manufacturing extract

200 g of dried seaweed was pulverized and then extracted with 2 L of 50% ethanol at room temperature for 24 hours. The extract was subjected to primary filtering with 8 μm filter paper, followed by secondary filtering with 3 μm filter paper. The final filtered extract solution was concentrated with a vacuum rotary evaporator and then powdered with a freeze dryer. The powdered sample is dissolved in 100% ethanol and used in the experiment.

<3-2> Anger  Extract Antioxidant  effect

Insect-derived cells in IPL-LD-65Y cells (IPL) and Jose town spore culture to 40,000 (FBS 10%, 250 units / ml penicillin, 250 ug / ml streptomycin TC-100 culture medium) was added to the Aster scaber (Aster scaber , 20 / / ml) and Artemisia lvandulaefolia , 20 / / ml), respectively. After 72 hours of incubation, the number of remaining NEGEMA was counted and the killing effect was tested.

As a result, it was found that the number of spores of N. jema was decreased when the sea urchin and mushroom extract were added to IPL (IPL-LD-65Y) cells as compared with the case where only N. japonica spore was added to IPL (LDL-65Y) cells.

<3-3> Antinodeagonal gene verification

The IPL-LD-65Y cells (IPL) and 40,000 spores of insect cells were added to the culture medium (FBS 10%, 250 units / ml penicillin, 250 ug / ml streptomycin TC-100 medium) ( Aster scaber , 20 占 퐂 / ml) and Artemisia lvandulaefolia , 20 [mu] g / ml).

After incubation for 72 hours, the surviving NEGEMA was recovered and DNA (GeneAll Exgene Plant SV mini) was extracted. The extracted DNA was amplified by NEGAME-specific primers (sense primer TTA CAG TAA GAG TGA GAC CTA TCA GC, anti-sense primer AAG TTA CCT CGC GTT GAG TC), followed by PCR (94 ° C for 15 seconds, 60 ° C for 30 seconds, and 72 ° C for 45 seconds).

As shown in Fig. 5, the expression of the Najema gene was reduced by the treatment of anchovy and mushroom extract (see Fig. 5).

<Example 4>

Anger  Extract Hang Ameba  effect

The IPL-LD-65Y cells (IPL) and 40,000 amebic cysts were added to the culture medium (FBS 10%, 250 units / ml penicillin, 250 ug / ml streptomycin TC-100 medium) Aster scaber , 20 占 퐂 / ml) and Artemisia lvandulaefolia , 20 / / ml). After 72 hours of incubation, the number of remaining amebic cysts was counted to determine the killing effect.

As a result, it was found that the number of amoeba cysts was remarkably reduced when the azalea extract was added to IPL (IPL-LD-65Y) cells (see FIG. 6).

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (5)

A pharmaceutical composition for preventing and treating a bee's nosema disease or amoeba disease, which contains an Aster scaber extract as an active ingredient. The method according to claim 1,
Wherein the flavor extract is extracted with a solvent selected from the group consisting of water, methanol, ethanol, hexane, butanol, ethyl acetate, chloroform and a mixed solvent thereof. The method according to claim 1,
Wherein said composition inhibits spore or amoebic cysts of Nogema &apos; s disease. A bee for the prevention and treatment of bee's nosema disease or amoeba disease containing an Aster scaber extract as an active ingredient. 5. The method of claim 4,
Wherein the beekeeping feed inhibits a spore of the Nogema disease or a cyst of the amoeba disease.

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