KR101900366B1 - Arsenic removal method of Hizikia fusiforme using organic acids - Google Patents

Abstract

The present invention relates to a method for removing arsenic from seaweeds. More specifically, the present invention relates to a method for removing arsenic by the following steps: adding an organic acid solution to seaweed and then treating ultrasound; adding water to the seaweed and treating heat; and adding an EDTA·2Na solution to the seaweed and then stirring the same thereafter. According to the method for removing arsenic, it is possible to remove 80 to 90% of a large amount of arsenic contained in the seaweed, thereby effectively reducing arsenic content, which has been a problem in exporting abroad. In addition, the arsenic removal method of the present invention can be useful in food fields without causing harm to human body with no chemical treatment undergone while minimizing deformation of the raw seaweed.

Description

(Arsenic removal method of Hizikia fusiforme using organic acids)

The present invention relates to a method for removing arsenic from seaweeds, and more particularly, to a method for removing arsenic from seaweeds, more particularly, a method of adding an organic acid solution to seaweeds followed by an ultrasonic treatment, a process of adding water to seaweeds followed by heat treatment, And then removing the arsenic by stirring the mixture.

It is rich in minerals such as calcium and iodine, and it contains a large amount of alginic acid and fucoidan as a main region in the southern coast of Korea (Jeju, Wando, Goheung, Jindo etc.) Prevention, has a variety of beauty effects.

In Japan, 4,500 ~ 5,000 tons of dry weight per year is used as a traditional food material consumed in Asia. In particular, it has been traditionally used in the form of rice dumplings or side dishes. In 2004, the Food Standard Agency 'FSA ), Tott has a lot of weapons arsenic, so it is advised to prohibit food.

Arsenic (AsIII), arsenate (AsV), arsenic monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenocholine (AsC) and arsenobetaine .

The toxicity of arsenic (AsIII), arsenate (AsV), arsenic monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenocholine (AsC) and arsenobetaine Exposure to large amounts of arsenic can lead to poisoning symptoms such as dizziness, headache, systemic pain, dyspnea, pigmentation, skin keratinization, diarrheal disorders, kidney and peripheral neuropathy.

Arsenic is present in the form of compounds in the water together with sulfur, copper, cobalt, lead and zinc, and is released into the environment in pesticides, pesticides, leather preservatives and glass manufacturing processes. .

Arsenic is present in the form of compounds in the water together with sulfur, copper, cobalt, lead and zinc, and is released into the environment in pesticides, pesticides, leather preservatives and glass manufacturing processes. .

The amount of arsenic present in seaweeds is rather high. Especially, such harmful arsenic is present in the contents of about 60 ~ 150ppm, and the treatment technique (arsenic removal technology) for it is insufficient. Therefore, an effective arsenic method is required for mass production of tofu.

In the case of seaweed, it has various parameters such as area, sunshine, salinity, and sampling time. Therefore, even if it is the same algae paper, it is likely that the arsenic removal parameters will vary because of different minerals, amino acids and general components.

Accordingly, the present inventor has established a method for removing arsenic contained in pots which can be scaled up as compared with the prior art, and completed the present invention as a technique that can be utilized as safe seaweed food.

Korean Patent Publication No. 10-2006-0096391

It is therefore an object of the present invention to provide a method for effectively removing arsenic from seaweed using an organic acid solution.

It is still another object of the present invention to provide a method for effectively removing arsenic from seaweed using heat treatment.

It is still another object of the present invention to provide a method for effectively removing arsenic from seaweeds using an EDTA · 2Na solution.

In order to achieve the above-mentioned object of the present invention,

The present invention provides a method for removing arsenic from seaweeds, comprising the step of adding an organic acid solution to the seaweed and then treating the ultrasonic waves.

In one embodiment of the present invention, the algae are selected from the group consisting of a foxtail, a foxtail, a tortoise, a dog gambler, a round spinning, a foxtail, a foxtail, a foxtail, a cortex, a gambler, a real gambler, , Seokgok, Jinyouari, Seaweed, Kelp, Vinegum, Minjitsu, Lacquer, Ringworm, Seaweed, Sephadose, Myphypia, Rhubarb, And may be at least one selected from the group consisting of parasitic, auditory, beaded hearing, jade and rosary.

In one embodiment of the present invention, the organic acid may be at least one selected from the group consisting of malic acid, tartaric acid, ascorbic acid, and citric acid.

In one embodiment of the present invention, the organic acid solution may be at a concentration of 1% (w / v).

In one embodiment of the present invention, the ultrasonic treatment may be ultrasonic treatment at a frequency of 30 to 50 KHz for 1 to 60 minutes.

In one embodiment of the present invention, the ultrasonic treatment may be ultrasonic treatment at a frequency of 40 KHz for 5 minutes.

In one embodiment of the present invention, the addition ratio of the algae and the organic acid solution may be 1: 500 (w / v).

The present invention also provides a method for removing arsenic from seaweeds, comprising adding water to seaweeds and then heat-treating the seaweeds at a temperature of 40 to 80 DEG C for 50 to 70 minutes.

In one embodiment of the present invention, the addition ratio of the algae and water may be 1: 100 (w / v).

In addition, the present invention provides a method for removing arsenic from seaweed, comprising adding seaweed to a solution of EDTA · 2Na and stirring for 1 to 12 hours.

In one embodiment of the present invention, the EDTA · 2Na solution may have a concentration of 0.01M to 0.1M.

In one embodiment of the present invention, the addition ratio of the seaweed and the EDTA · 2Na solution may be 1: 100 (w / v).

The arsenic removal method of the present invention can remove 80 to 90% of a large amount of arsenic contained in seaweeds, thereby effectively reducing arsenic content, which has been a problem in exporting from abroad. In addition, the arsenic removal method of the present invention has an advantage that the deformation of the seaweed basin is minimized and the chemical treatment is not performed, so that it is not harmful to the human body and can be usefully used in the food field.

(A), (b), (C), (C), and (C), respectively. FIG. 1 is a SEM photograph of the surface of the roots prepared in one embodiment of the present invention. (D) Ultrasonically treated toothpaste in 1% tartaric acid.
(A), (b), (c), (d), and (d) 0.1M EDTA, respectively. In the present invention, · Dipped in 2Na solution, (d) immersed in 0.01M EDTA · 2Na solution.
FIG. 3 shows the structural formula of the arsenic species of the present invention.
Fig. 4 shows the principle of processing the lead oxide prepared in the embodiment of the present invention.
5 shows the structural formula of the organic acid used in the present invention.
FIG. 6 is a schematic diagram showing the arsenic removal method used in the present invention.

The present invention is characterized by providing a method for effectively removing arsenic from seaweeds.

The seaweed of the present invention may be selected from the group consisting of red algae, brown algae and green algae.

The red algae may be exemplified by the following: red ginseng, red ginseng, red ginseng, round ginseng, ginseng, ginseng, ginseng, ginseng, gambling, gashu, silkworm, .

The brown algae can be exemplified by seaweed, kelp, horses, horsetail, horsetail, horsetail, horsetail, horsetail, moxa, rhubarb, southern cousin, moth, hornfish,

Examples of the green algae include chrysanthemum, chestnut, chestnut, hearth, beard, jade, and salt weekly.

Accordingly, the seaweed of the present invention can be used as a seaweed extract of the present invention, which can be applied to a variety of seaweeds such as mugwort, kim, kotoni, dog gambling, round spinning, There are many kinds of seaweeds such as seaweed, seaweed, kelp, horses, horses, lobsters, horsetail, seaweed, Bee hearing, jade, and rosemary. In the following examples, experiments were conducted to remove arsenic from roots.

First, the present invention provides a method for removing arsenic from seaweed, comprising adding an organic acid solution to seaweeds and then treating ultrasonic waves.

In one embodiment of the present invention, the organic acid may be selected from the group consisting of malic acid, tartaric acid, ascorbic acid and citric acid, and the organic acid solution may be a concentration of 1 to 5% (w / v).

In another embodiment of the present invention, the treated ultrasonic treatment conditions may be ultrasonic treatment at 290 to 350 W intensity and 30 to 50 KHz frequency for 1 to 60 minutes, preferably at 290 to 350 W and 40 KHz, To treat the ultrasonic wave for ~ 60 minutes, more preferably to treat the ultrasonic wave for 5 minutes at the 290 ~ 350W intensity and the 40KHz frequency.

In another embodiment of the present invention, the ratio of the algae to the organic acid solution may be 1:20 to 1: 500 (w / v), preferably 1: 500 (w / v).

The present invention also provides a method for removing arsenic from seaweeds, comprising adding water to seaweeds followed by heat treatment.

In detail, arsenic can be removed from the seaweed by adding water to the seaweed and then heat-treating it at a temperature of 40 to 80 ° C for 50 to 70 minutes.

In one embodiment of the invention, the addition rate of seaweed and water may be 1: 100 (w / v).

Further, there is provided a method for removing arsenic from seaweed, comprising adding seaweed to a solution of EDTA · 2Na and stirring for 1 to 12 hours.

In one embodiment of the present invention, the EDTA · 2Na solution may have a concentration of 0.01M to 0.1M.

In another embodiment of the present invention, the addition ratio of the seaweed to the EDTA · 2Na solution may be 1: 100 (w / v).

In another embodiment of the present invention, the stirring step may be carried out at a temperature of 25 to 35 DEG C under stirring at 100 to 200 rpm for 10 to 15 hours.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example >

material

In the following experiment, Tott was purchased directly from Wando; Malic acid, tartaric acid, ascorbic acid, citric acid and EDTA · 2Na were purchased from Daejeong Chemical.

< Example  1>

Component analysis

<1-1> General composition analysis

General compositional analysis was performed on the roots. Analysis of the components was carried out on moisture, protein, fat, carbohydrate and ash, and the results are shown in Table 1 below. Moisture, fat, protein and ash (%) were measured according to the AOAC method (1990).

Common components of potato ingredient
sample moisture Ash protein Fat carbohydrate Leaves 10.58 14.85 11.04 0.4 63.13 Raw stalk 11.67 15.50 9.49 0.6 62.74

As a result of the analysis, there was almost no difference between the general components of the leaves and stems of the green tea leaves, and the carbohydrate component was the most important value.

<1-2> Mineral composition analysis

First, the change of minerals content of the pottery was analyzed by immersion treatment at the ratio of 1: 100 to the general distilled water. In addition, the change of mineral content of red pepper was analyzed according to the addition ratio of red pepper and distilled water for 1 hour at room temperature. Mineral content was measured using ICP-MS (Nexion®300X, Perkin Elmer).

Changes in mineral content over time after soaking in distilled water ingredient Sample Raw material Distilled water
30 minutes Distilled water
1 hours Distilled water
3 hours Distilled water
6 hours Distilled water
12 hours Fe 480.10 180.59 88.00 146.95 130.10 207.11 Cr 1.49 5.90 3.08 3.80 2.96 2.23 Cu 2.66 7.11 7.77 3.42 3.46 12.52 Mn 25.93 29.52 17.45 19.61 18.61 28.49 Ni 4.68 3.60 2.86 2.98 3.02 4.58 Zn 11.53 18.73 10.28 10.06 10.14 13.30 CD 2.10 1.90 1.94 1.64 1.45 2.39 Pb 0.51 0.78 0.32 0.34 0.32 0.58 As 91.12 54.13 31.71 29.63 30.66 36.91 Co 0.40 0.57 0.31 0.31 0.33 0.44 P 1994.00 929.50 859.50 808.00 848.50 1099.50 Na 10090.00 10835.00 14400.00 14170.00 13510.00 10730.00 Ca 13270.00 16435.00 24610.00 23740.00 23000.00 16565.00 Mg 5283.00 8430.00 14980.00 13945.00 14210.00 8400.00 K 54950.00 29465.00 52450.00 50850.00 49630.00 28890.00

As a result of analysis, it was confirmed that the content of arsenic contained in Tott is 91.12 ~ 117.65ppm, which is about 100ppm of arsenic. It was confirmed that the arsenic content of the roots was reduced to 30 ppm by the water treatment after immersion treatment in the distilled water at room temperature. In case of immersion treatment at room temperature, it was confirmed that the equilibrium was almost equal to the amount of arsenic after 1 hour.

According to the analysis in Table 3 above, when the dipping treatment was carried out at room temperature for 1 hour, it was determined that the content of minerals was proportional to the ratio of water to water.

Changes in Mineral Content of Chrysanthemum indicum according to the Addition Rate of Chopped and Distilled Water ingredient The addition ratio (w / v) 1:25 1:50 1:75 1: 100 Fe 268.27 171.77 154.18 88.00 Cr 4.02 3.49 3.89 3.08 Cu 11.69 8.74 13.87 7.77 Mn 49.74 37.26 34.84 17.45 Ni 4.84 3.33 6.99 2.86 Zn 7.74 8.23 8.95 10.28 CD 2.12 2.65 1.93 1.94 Pb 1.44 1.07 1.06 0.32 As 34.48 34.70 27.77 31.71 Co 0.73 0.59 0.60 0.31 P 2049.60 1932.80 2009.20 859.50 Na 11122.60 11304.60 11969.40 14400.00 Ca 9707.40 9688.20 9912.20 24610.00 Mg 7725.40 7789.20 7903.00 14980.00 K 30122.40 30989.60 29121.00 52450.00

As a result, the lowest arsenic content was confirmed at a ratio of 1:75, and the ratio of 1:25 was judged to be suitable for the treatment of a large amount of tars, since there was no significant difference at other ratios.

< Example  2>

How to remove arsenic from brown algae

To remove arsenic from brown algae effectively, arsenic removal by ultrasonic treatment, arsenic removal by heat treatment at various temperature conditions, and removal of arsenic by using EDTA · 2Na solution I looked at it.

&Lt; 2-1 >

The tops were soaked in normal temperature water for 1 hour and then washed three times. The swelled tops were used in the following experiments.

<2-2> Application of various organic acids to ultrasonic treatment Arsenic removal

The swelled tablets prepared in Example <2-1> were immersed in a solution of various 1% (w / v) organic acids (malic acid, tartaric acid, ascorbic acid, citric acid), and then ultrasonic devices ( POWERSONIC 420, HWASHIN ) Ultrasonic treatment was performed at a frequency of 290 to 350 W at a frequency of 40 KHz for 1 to 60 minutes. The addition ratio of top and organic acid solution was 1: 500 (10 g of top, 5 L of each solution).

<2-3> Removal of arsenic by heat treatment at various temperature conditions

The swollen roots prepared in Example <2-1> were immersed in water and then heat-treated at room temperature (15 to 25 ° C.), 40, 50, 60, 70, and 80 ° C. for 1 hour, respectively. The addition ratio of water to water was 1: 100 (10 g of water, 1 L of water).

<2-4> EDTA · 2Na  Solution Arsenic removal

The swollen roots prepared in Example <2-1> were immersed in 0.01M to 0.1M EDTA · 2Na solution and stirred at 100 rpm for 1 to 12 hours in a shaking stirrer at 30 ° C. The addition ratio of the solution of chitosan and EDTA · 2Na solution was 1: 100 (10 g of total solution, 1 L of EDTA · 2Na solution).

< Example  3>

The application of organic acid to the ultrasound- Arsenic removal  effect

<3-1> Changes in Mineral Content of Chamaecyparis obtusa according to Organic Acid

The changes in mineral content of the red pepper after the treatment of Example <2-2> were measured, and the results are shown in Table 4 below. Ultrasonic waves were treated for 1 hour.

The mineral content of the roots subjected to ultrasonic treatment by applying various organic acids ingredient Sample Distilled water Malian Tartaric acid Ascorbic acid Citric acid Fe 88.93 141.75 52.96 53.66 147.75 Cr 2.05 3.89 2.49 3.17 1.74 Cu 11.34 5.59 5.98 15.17 7.67 Mn 24.72 9.15 15.18 15.60 18.82 Ni 4.72 1.86 2.15 2.79 3.41 Zn 14.52 5.78 3.62 10.95 11.86 CD 2.01 0.84 0.53 1.21 1.60 Pb 0.50 0.65 0.65 2.51 0.67 As 30.55 14.39 13.52 13.29 13.07 Co 0.37 0.28 0.24 0.29 0.24 P 1083.50 715.00 600.00 537.50 732.00 Na 11240.00 649.50 549.00 1137.50 51600.00 Ca 17260.00 12605.00 11075.00 20030.00 16860.00 Mg 8845.00 5030.00 4711.50 10045.00 5280.00 K 29810.00 3002.50 2089.50 5005.00 2559.50

When ultrasonic treatment was performed on distilled water, a trace amount of arsenic was removed. On the other hand, when ultrasonic treatment was performed on each of ascorbic acid, malic acid, tartaric acid and citric acid as organic acids, low arsenic removal was effectively performed, I could.

<3-2> Changes in Mineral Content of Tarts with Ultrasonic Treatment Time

In the meantime, it has been confirmed through the above experiments that the ultrasonic treatment is effective in removing arsenic by immersing the organic acid solution in the organic acid solution compared to the distilled water. In addition, in order to determine the optimum ultrasonic time condition, The arsenic content of the red pepper was measured according to the ultrasonic time.

Mineral content of top in 1% ascorbic acid by ultrasonic treatment ingredient Ultrasonic treatment 1 minute 2 minutes 3 minutes 5 minutes 15 minutes 30 minutes 60 minutes Fe 405.50 255.34 178.05 191.54 220.87 102.69 53.66 Cr 4.27 3.54 2.75 4.43 4.43 3.41 3.17 Cu 8.41 8.29 7.49 22.11 32.31 23.45 15.17 Mn 27.00 27.32 25.81 45.78 37.05 21.05 15.60 Ni 2.50 2.87 2.66 4.77 4.03 3.39 2.79 Zn 20.17 27.78 14.35 8.71 8.29 6.34 10.95 CD 2.88 2.73 1.84 2.20 1.88 2.56 1.21 Pb ND ND ND 1.52 2.95 1.67 2.51 As 46.99 51.93 40.18 10.86 16.85 14.59 13.29 Co 0.34 0.41 0.39 0.75 0.68 0.48 0.29 P 730.50 894.00 853.50 1971.80 2008.60 1822.20 537.50 Na 6385.00 5850.00 6670.00 3826.80 1712.80 1106.40 1137.50 Ca 14485.00 15080.00 15660.00 4761.00 7676.20 6658.20 20030.00 Mg 6695.00 6885.00 7480.00 6443.60 4648.60 3866.20 10045.00 K 17820.00 15930.00 15640.00 9733.40 3685.60 1812.80 5005.00

As a result, as shown in Table 5, there was no significant difference up to 3 minutes after the ultrasonic treatment, and the remarkable superior arsenic removal effect was confirmed in the experimental group treated with the ultrasonic wave for 5 minutes. In particular, the effect of arsenic treatment was further reduced in the experimental group treated with ultrasound for 15 minutes, 60 minutes, and 5 minutes compared with the group treated with ultrasonic waves, and it was confirmed that the treatment efficiency of arsenic was not dependent on the ultrasonic treatment time. The ultrasonic treatment conditions as described above were judged to have significance as a threshold value in which the arsenic removal effect was abruptly changed with a specific numerical value as a boundary.

<3-3> Variation of mineral content of red pepper depending on the ratio of organic acid solution applied to red pepper

In order to determine the optimum ratio of 1% (w / v) ascorbic acid solution, the ratio of organic acid solution applied to the roots was varied and sonicated for 5 minutes to analyze the mineral content of roots.

Mineral content of top in 1% ascorbic acid by ratio sonication ingredient The ratio (w / v) of the solution of top and ascorbic acid 1:20 1:50 1: 100 1: 200 1: 500 Fe 751.26 168.95 246.10 141.37 191.54 Cr 5.99 2.97 4.90 3.99 4.43 Cu 9.64 4.76 14.13 10.90 22.11 Mn 39.70 36.78 36.01 28.30 45.78 Ni 3.83 2.64 3.57 4.80 4.77 Zn 30.25 14.95 32.03 16.62 8.71 CD 2.87 3.09 2.83 1.80 2.20 Pb 0.81 ND 0.14 0.03 1.52 As 53.75 44.90 54.63 35.48 10.86 Co 0.61 0.44 0.53 0.34 0.75 P 1035.50 714.00 918.50 574.50 1971.80 Na 6670.00 8800.00 4681.50 7905.00 3826.80 Ca 15745.00 17930.00 17350.00 15545.00 4761.00 Mg 7790.00 9240.00 7640.00 7650.00 6443.60 K 19075.00 24915.00 12570.00 21985.00 9733.40

The lowest arsenic content of 1: 500 was obtained by ultrasonication for 5 minutes at 1:20, 1:50, 1: 100, 1: 200 and 1: 500 ratio of 1% (w / v) ascorbic acid And there was no significant removal effect in the remaining ratio.

< Example  4>

Through heat treatment Arsenic removal  effect

The changes in the mineral content of the roots after the treatment of Example <2-3> were measured, and the results are shown in Table 7 below.

Mineral content of pottery during heat treatment at 40 ~ 80 ℃ ingredient Heat treatment temperature 40 ℃ 50 ℃ 60 ° C 70 ℃ 80 ℃ Fe 520.62 334.89 256.38 184.22 152.60 Cr 3.79 2.55 1.97 1.94 1.99 Cu 9.20 6.00 5.26 4.49 3.87 Mn 67.44 52.98 50.33 46.17 47.73 Ni 6.72 4.70 4.27 3.47 3.60 Zn 13.96 7.60 7.63 12.23 4.53 CD 2.57 1.64 1.33 1.15 0.88 Pb 0.56 0.35 0.21 0.22 0.16 As 48.18 25.50 19.41 13.42 11.50 Co 0.57 0.36 0.39 0.24 0.22 P 2212.80 1900.40 2214.40 2131.60 2189.80 Na 9588.40 11753.00 10957.60 12047.80 13077.00 Ca 8707.40 9342.80 9737.40 9780.40 10216.20 Mg 7173.40 8035.60 8318.60 8812.40 9377.20 K 24447.20 23985.40 26696.20 27022.80 26625.20

As a result, as shown in Table 7, it was confirmed that the arsenic content was lowered when the heat treatment was carried out from 40 ° C to 80 ° C. From these results, it can be confirmed that the heat treatment at high temperature after immersion in water is effective for removing arsenic.

< Example  5>

EDTA · 2Na  Through solution Arsenic removal  effect

The changes in the mineral content of the red pepper after the treatment of Example <2-4> were measured, and the results are shown in Table 8 below.

Mineral content of top in 0.1 M or 0.01 M EDTA · 2Na solution at 30 ° C ingredient Treatment condition 0.01M EDTA · 2Na
Immersion for 1 hour 0.01M EDTA · 2Na
3 hours immersion 0.01M EDTA · 2Na
6 hours immersion 0.01M EDTA · 2Na
12 hours immersion 0.1M EDTA · 2Na
12 hours immersion Fe 455.75 286.75 203.74 223.98 270.96 Cr 8.525 4.94 3.31 3.275 2.38 Cu 4.645 8.93 9.49 2.025 3.55 Mn 8.195 5.03 4.02 2.79 3.37 Ni 3.34 2.21 2.31 1.715 1.21 Zn 19.775 2.86 3.33 3.605 3.91 CD 0.325 0.11 0.08 0.07 0.10 Pb 0.275 0.63 0.62 0.09 0.13 As 48.315 25.52 23.55 15.36 25.15 Co 0.55 0.43 0.37 0.225 0.20 P 983.5 2002.80 2101.80 688.5 1064.50 Na 22115 23655.60 23122.80 27760 57350.00 Ca 13140 6727.20 6521.20 14120 2622.50 Mg 8180 7146.80 7250.80 12725 1892.00 K 18680 15782.60 19509.20 31900 8210.00

As a result, as shown in Table 8, low arsenic content was confirmed in 0.01M EDTA · 2Na solution than 0.1M EDTA · 2Na, and 12 hours was most suitable for EDTA · 2Na treatment.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (12)

(a) immersing seaweed in water for 1 hour, followed by washing with water to swell; And
(b) adding an ascorbic acid solution at a concentration of 1 to 5% (w / v) to the swollen seaweed, and then treating the ultrasonic wave at a frequency of 290 to 350 W and a frequency of 30 to 50 KHz for 5 minutes,
Wherein the mixing ratio of the seaweed swollen in step (b) and the ascorbic acid solution is 1: 500 (w / v). The method according to claim 1,
The above seaweeds may be selected from the group consisting of seaweeds, fennel, kotoni, dog gambling, round starch, , Heavenly horses, horses, horses, lobes, horseshoe, sea bass, horseshoe, mosquito, rhubarb, rhinoceros cousin, And at least one member selected from the group consisting of a salt and a salt. delete delete delete delete delete delete delete delete delete delete

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