KR101871314B1 - Composition for anti-obesity containing seeweed extract - Google Patents

Abstract

The present invention relates to an anti-obesity composition containing a seaweed extract for preventing and improving obesity. More particularly, the present invention relates to a composition for an anti-obesity containing a seaweed extract, a gentian extract, a garcinia cambogia extract, Extract, and the like, which can improve the preservability with improved weight loss and antioxidant effect, and which is composed of a plant extract and contains a marine extract harmless to human body.

Description

TECHNICAL FIELD The present invention relates to an anti-obesity containing seeweed extract,

The present invention relates to an anti-obesity composition containing a seaweed extract for preventing and improving obesity. More particularly, the present invention relates to a composition for an anti-obesity containing a seaweed extract, a gentian extract, a garcinia cambogia extract, Extract, and the like, which can improve the preservability with improved weight loss and antioxidant effect, and which is composed of a plant extract and contains a marine extract harmless to human body.

Obesity results in diverse metabolic diseases such as diabetes, hypertension and heart disease, which are caused by an increase in fat cell mass and an increase in adipose tissue mass due to an increase in fat cell size. Adipocyte differentiation leading to obesity is a complex process accompanied by changes in morphology, hormone sensitivity and gene expression. Fusion adipose precursor cells express adipocyte markers by clonal proliferation, growth arrest and hormone induction, . Therefore, as shown in the following Patent Literature, it is possible to inhibit the formation of fat from the fibroblast precursor cells into mature adipocytes and to induce the lipolysis in adipose tissue, that is, to reduce the amount of adipose tissue to prevent obesity Compositions are being developed.

<Patent Literature>

Patent Publication No. 10-0645385 (published on November 23, 2006) "Composition for inhibiting obesity"

However, the composition for anti-obesity containing the extract of Artemisia japonica has a small effect, so that the anti-obesity effect can not be expected substantially. Therefore, it is urgently required to develop a novel composition which is safe to the living body and is more effective than the conventional composition for anti-obesity.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems,

It is an object of the present invention to provide an anti-obesity composition containing a seaweed extract which can prevent and improve obesity.

It is another object of the present invention to provide an anti-obesity composition which can further provide an antioxidative effect and increase the effect of weight loss, further comprising a gangsia extract, Garcinia cambogia extract and dietary fiber.

It is another object of the present invention to provide a composition for anti-obesity that can further improve preservability by containing licorice extract.

In order to achieve the above object, the present invention is implemented by the following embodiments.

According to one embodiment of the present invention, the composition for anti-obesity according to the present invention is characterized by including a seaweed extract, a Ganoderma extract, a Garcinia cambogia extract and a dietary fiber.

According to another embodiment of the present invention, the composition for anti-obesity according to the present invention comprises 0.1 to 20 parts by weight of Ganoderma extract, 0.1 to 20 parts by weight of Garcinia cambogia extract and 0.1 to 60 parts by weight of dietary fiber, Is used.

According to another embodiment of the present invention, the composition for anti-obesity according to the present invention further comprises 0.01 to 10 parts by weight of licorice extract, based on 100 parts by weight of the marrow extract.

According to another embodiment of the present invention, the composition for anti-obesity according to the present invention is characterized by being used as a food additive.

According to still another embodiment of the present invention, the composition for anti-obesity according to the present invention is characterized in that it is obtained by hot water extraction in a pressurized heating state.

According to still another embodiment of the present invention, the composition for anti-obesity according to the present invention is characterized in that the wakamisu extract has a liquid or powder form.

According to the present invention, the following effects can be obtained by this embodiment.

The present invention has the effect of preventing and improving obesity by containing seaweed extract.

The present invention further provides an antioxidant effect and an effect of weight loss by further containing gangsiae extract, Garcinia cambogia extract and dietary fiber.

Further, the present invention has the effect of enhancing preservability by further including licorice extract.

1 is a graph showing the cytotoxicity evaluation of an anti-obesity composition according to an embodiment of the present invention.
FIG. 2 is a graph showing the evaluation of liposuction inhibitory effect of an anti-obesity composition according to an embodiment of the present invention. FIG.
FIG. 3 is a graph showing the evaluation of the effect of inhibiting triglyceride production of an anti-obesity composition according to an embodiment of the present invention.

Hereinafter, the anti-obesity composition containing the seaweed extract according to the present invention will be described in detail with reference to the accompanying drawings. Unless defined otherwise, all terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs and, if conflict with the meaning of the terms used herein, It follows the definition used in the specification. Further, the detailed description of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted. Throughout the specification, when an element is referred to as "including " an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

The composition for anti-obesity according to one embodiment of the present invention includes a seaweed extract, a gentian extract, a Garcinia cambogia extract and a dietary fiber. The composition for anti-obesity can be prepared in any formulations conventionally produced in the art including liquid and powder, and can be used for various purposes including food additives and the like.

The seaweed has the function of cleansing the blood, restoring the ki, activating the metabolism, improving rough skin, improving constipation, and has become a necessary postpartum food. In addition, the seaweed has nutrition and functions necessary for human being, and it has been used as a functional food and a favorite food for the general public. In the seaweed, it contains all the minerals that our body needs, and the content of the minerals contains minerals , Alginic acid and fucoidan contained in the seaweed is a functional substance contained in the slippery viscous substance. It is a functional substance that reduces cholesterol, prevents and improves hypertension and atherosclerosis, improves and prevents diabetes, prevents and treats constipation, , Anti-cancer, immunity enhancement, ulcer, mucosal repair of the stomach wall, sterilization of pathogenic bacteria, and so on. The composition for anti-obesity according to the present invention contains a seaweed extract having an effect of inhibiting the production of lipids and triglycerides. Meanwhile, the seaweed extract according to the present invention can be prepared as follows. The wakame extract of liquid phase can be obtained through hot water extraction using purified water in a pressurized heating state, and the liquid wakame extract can be concentrated under reduced pressure and lyophilized to produce granular powder. The method of extracting hot water under a pressurized state, concentrating under reduced pressure, and lyophilizing is performed by a known method, and thus a detailed description thereof will be omitted.

Phaeophytaae Phaeophyceae Laminariales A perennial brown algae belonging to Alariaceae. It is a deep-seated deep-sea creature which inhabits a cylindrical shape with a length of 1 ~ 2m, Stem length 5 ~ 10cm, diameter 3 ~ 5mm, rosette leaf length 20 ~ 30cm, width 4cm is about. The chewing gum extract is used in an amount of 0.1 to 20 parts by weight based on 100 parts by weight of the seaweed extract.

Garcinia cambogia is one of the Guttifera and Garcinia species. The peel contains 10-30% of HCA (Hydroxy Citric Acid). HCA is a very similar material to citric acid in orange or other citrus , The species with the highest content of HCA is known as Garcinia cambogia. The Garcinia cambogia extract is used in an amount of 0.1 to 20 parts by weight based on 100 parts by weight of the seaweed extract, and is obtained from the Garcinia cambogia skin. The ghatti extract and Garcinia cambogia extract can be obtained in the same manner as the seaweed extract, so a detailed description thereof will be omitted.

The dietary fiber refers to a pollutant-digesting polymeric fiber component that is not decomposed by the digestive enzymes of the body. It is mainly distributed on the cell wall of the plant cell or on the skin of the plant seed. Its function is to prevent constipation, hemorrhoids, colon cancer and appendicitis It is effective and can prevent circulatory mechanical diseases such as hypertension, arteriosclerosis, and heart disease, and inhibits the increase of blood glucose level and has a good effect on diabetes. The dietary fiber may be used in an amount of 0.1 to 60 parts by weight based on 100 parts by weight of the seaweed extract, and the dietary fiber may be a commercially available product in powder form. The present invention further improves the antioxidative effect by improving obesity even when the same amount of extract is used, including the gentian extract, Garcinia cambogia extract and dietary fiber, in addition to the seaweed extract having the anti-obesity effect.

The anti-obesity composition according to another embodiment of the present invention may further comprise licorice extract to improve the preservability. The licorice extract is used in an amount of 0.01 to 10 parts by weight based on 100 parts by weight of the seaweed extract, and can be obtained in the same manner as the seaweed extract.

Glycyrrhizin is a perennial herb that belongs to soybean and contains 6 ~ 14% of glycyrrhizin, which is a sweet component. It has been proved for a long time since its safety has been proved for almost all herbal ingredients. Glycyrrhizin, It is effective against allergies, chronic hepatitis and viral diseases. It is rich in flavonoids such as glabridin, liquiritigenin and liquiritin and is widely used in foods, medicines and cosmetics. .

The composition for anti-obesity may further contain a plant extract in which various plant components are mixed in place of the licorice extract to improve the preservability, and the plant extract may be used in an amount of 0.1 to 1.5 parts by weight per 100 parts by weight of the seaweed extract The plant extracts were prepared by immersing the plant extracts mixed with the same weight, the root of the herbaceous plant, the chestnut root, the chestnut fruit, and the camphor leaf in a vinegar (10 to 20 g of stone outer leaves per liter of vinegar, 10 to 20 g of rootstock, 20 to 20 g and camphor leaf 10 to 20 g) are mixed and held at a temperature of 10 to 15 캜 for 2 to 3 days, and the extract is removed by freeze-drying the obtained extract.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these are only for the purpose of illustrating the present invention in more detail, but the scope of the present invention is not limited thereto.

In the following examples, the cytotoxicity evaluation of an anti-obesity composition, liposuction inhibition efficacy evaluation, neutral lipid production inhibition efficacy evaluation, polyphenol and flavonoid content evaluation, and antioxidant activity will be examined. 3T3-L1 adipocyte precursor cells, which are used for studying adipocyte differentiation, were used for the cytotoxicity evaluation, evaluation of inhibition of lipogenesis, and evaluation of inhibition of triglyceride production. Experiments to evaluate the efficacy of the following anti-obesity composition were carried out using methods commonly used in the art.

<Example 1> Preparation of sample for anti-obesity composition

(1) The seaweed was washed, immersed in purified water (purified water was used five times as much as the seaweed), and heated at 80 캜 for 5 hours with stirring to obtain a liquid extract. The liquid extract was concentrated under reduced pressure And then lyophilized to prepare powdery seaweed extract. In the same manner as in the case of obtaining the above sea mustard extract, ganoderma extract, Garcinia cambogia bark extract and licorice extract were prepared. In addition, a commercial powdered dietary fiber was prepared. Plant extracts were prepared by immersing the leaves of the stems, the root of the stems, the fruit of the females, and the camphor leaves in vinegar (30g of stone outer leaves per 30g of vinegar, 30g of rootstock, 30g of chestnut fruit and 30g of camphor leaf) The mixture was maintained for 3 days, and the extract was removed. The obtained extract was concentrated under reduced pressure and lyophilized to prepare.

(2) The above-prepared extracts and dietary fiber were mixed in the ratios shown in Table 1 below to prepare samples for anti-obesity composition.

ingredient Content (% by weight) Sample 1 Sample 2 Sample 3 Sample 4 Seaweed extract 100 91 90 90 Moth extract 0 3 3 3 Garcinia cambogia bark extract 0 3 3 3 Dietary Fiber 0 3 3 3 Licorice extract 0 0 One 0 Plant extract 0 0 0 One

<Example 2> Evaluation of cytotoxicity of composition for anti-obesity

(1) To determine the degree of cytotoxicity of the composition for anti-obesity, 3T3-L1 preadipocytes were treated with MTT assay.

(2) FIG. 1 shows the result of MTT assay in which sample 1 was treated. In FIG. 1, when the sample 1 is treated at a concentration of 200 ug / ml or less, the survival rate of the control group without the anti- And survival rate. In addition, in the case of Samples 2 to 4, it was also confirmed that no cytotoxicity was observed at a concentration of 200 ug / ml or less.

&Lt; Example 3 > Evaluation of liposuction inhibitory effect of the composition for anti-obesity

(1) To examine the effect of the composition for anti-obesity on the lipid droplet production inhibition, 3T3-L1 preadipocytes were treated with a certain concentration of each sample, After staining with Oil red O, a dyeing reagent that specifically stained the cells, the absorbance was measured by eluting the stained lipids using 100% isopropyl alcohol. The amount of lipid produced was compared with the standard of lipid generated in the control group not treated with anti-obesity composition, and the results are shown in FIG. 2 and Table 2 below.

(2) FIG. 2 shows the result when Sample 1 is treated. As shown in FIG. 2, it can be seen that as the concentration increases up to 200 ug / ml, the amount of lipid peroxide decreases. Table 2 shows the relative proportions of the amounts of fat globules produced after treating samples 1 to 4 having a concentration of 200 ug / ml. In Table 2, it is seen that the samples 2, 3, 4 and 5, which additionally contain Ganoderma extract, Garcinia cambogia extract and dietary fiber, To 4 significantly inhibited the production of lipid sphere as compared to the sample 1 containing only the seaweed extract.

Control group (untreated sample) Sample 1 Sample 2 Sample 3 Sample 4 Fat Sphere Production (%) 100 76 60 58 62

&Lt; Example 4 > Evaluation of the effect of inhibiting the formation of triglyceride in an anti-obesity composition

(1) Triglyceride is used as an important energy source of cells together with glucose, but extra triglyceride is absorbed and stored in fat cells and acts as a cause of obesity. Therefore, triglyceride production In order to examine the inhibitory effect, 3T3-L1 preadipocytes were treated with a certain concentration of each sample, quantified using a Triglyceride assay kit, and the absorbance was measured. The amount of triglyceride produced was compared with the standard of the triglyceride produced in the control group not treated with the anti-obesity composition as a standard, and the results are shown in Fig. 3 and Table 3 below.

(2) FIG. 3 shows the result when Sample 1 is treated. FIG. 3 shows that as the concentration increases to 200 ug / ml, the amount of triglyceride decreases. Table 3 shows the relative proportions of the amounts of triglyceride produced after each treatment of Samples 1 to 4 having a concentration of 200 ug / ml. Table 3 shows that the samples containing a gentian extract, Garcinia cambogia extract and dietary fiber 2 to 4 significantly suppressed the production of triglyceride as compared with the sample 1 containing only the seaweed extract.

Control group (untreated sample) Sample 1 Sample 2 Sample 3 Sample 4 Production of triglyceride (%) 100 52 38 36 37

Example 5 Evaluation of Polyphenol and Flavonoid Content of Anti-Obesity Composition

(1) The contents of polyphenols and flavonoids were evaluated for Samples 1 to 4, and the results are shown in Table 4. The total polyphenol content was measured by Folin-Denis method. The amount (mg / g) was determined by quantitative curve using tannic acid (Sigma, USA) as indicator material. Total flavonoid content was measured by AlCl ₃ method And quantified using quantitative curves generated using quercetin (Sigma, USA) as the indicator material.

(2) Table 4 shows that the contents of polyphenol and planovoid are significantly higher than that of sample 1, in which samples 2 to 4, which additionally contain Gentiana extract, Garcinia cambogia extract and dietary fiber, have a marine extract alone.

Content (mg / g) Sample 1 Sample 2 Sample 3 Sample 4 Total polyphenol content 2.89 ± 0.14 3.89 ± 0.13 3.94 + - 0.15 4.01 + - 0.14 Total flavonoid content 3.26 ± 0.11 4.26 ± 0.10 4.46 0.10 4.44 0.11

&Lt; Example 6 > Evaluation of Antioxidant Efficacy of Anti-Obesity Composition

(1) Antioxidant efficacy was evaluated for each of the samples 1 to 4 having a concentration of 10 mg / ml, and the results are shown in Table 5. The antioxidant activity was measured by the Blois method, which measures the radical scavenging effect of 1,1-diphenyl-2-picrylhydrazyl (DPPH).

(2) As shown in Table 5, the antioxidative activity of the samples 2 to 4, which additionally contains the Gentiana extract, Garcinia cambogia extract and dietary fiber, is higher than that of the sample 1 containing only the seaweed extract.

Sample 1 Sample 2 Sample 3 Sample 4 DPPH (%) 3.89 ± 0.44 8.39 ± 0.23 7.88 ± 0.50 7.66 ± 0.91

&Lt; Example 7 >

(1) Evaluation of the effect of increasing the storage period by the experiment of measuring the number of general bacteria

Samples 1 to 4 were diluted 10-fold with tap water and allowed to stand at 30 ° C for 3 days, diluted 10-fold each, inoculated 0.1 ml onto sterilized standard methods agar (Difco) and incubated at 35 ° C for 36 hours The number of colonies was counted by counting the number of colonies generated after the colonies were generated from 30 to 300 colonies per plate. The number of colonies was counted, and the number of bacteria was calculated by multiplying the number of colonies by the dilution factor. Sample 4, sample 3, sample 1, (The number of bacteria in the sample 4 is much smaller than that of the other samples, and the number of bacteria in the sample 3 is much smaller than that of the samples 1 and 2). This shows that the inhibition of bacterial growth when the licorice extract is used and the inhibition of bacterial proliferation remarkably when the plant extract is used.

(2) Evaluation of effect of preservation period increase by sensory test

Samples 1 to 4 were diluted 10-fold with tap water, stored in an environment of 35 ° C and 80% humidity, and 10 days of the general public were checked for odor every day, and the date when an unpleasant smell was started was confirmed. In case of sample 1, an unpleasant smell began to appear after one day, and in sample 2, an unpleasant odor began to appear after 1.2 days. In sample 3, after 2 days, an unpleasant odor began to appear , And sample 4 had an unpleasant smell after 4 days. This shows that when the licorice extract is used, the decay is slowed down, and when the plant extract is used, the decay is slowed considerably.

 While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Should be interpreted as belonging to the scope.

Claims (7)

Sea mustard extract, gentian extract, Garcinia cambogia extract, dietary fiber and plant extract,
The seaweed extract, Ganoderma lucidum extract, Garcinia cambogia extract, dietary fiber and plant extract were mixed at a weight ratio of 90: 3: 3: 3: 1,
The plant extract was prepared by mixing 30 g of stone outer leaves per liter of vinegar, 30 g of root, 30 g of chestnut fruit, and 30 g of camphor leaf, and maintaining the mixture at a temperature of 10 to 15 캜 for 2 to 3 days. Or a pharmaceutically acceptable salt thereof. delete delete delete delete delete delete

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