KR101862742B1 - Composition for improvement and prevention of gastroesophageal reflux disease and method for preparing the same - Google Patents

Abstract

Provided are a composition for alleviating and preventing reflex esophagitis and gastritis and a method for preparing the same. The composition for alleviating and preventing reflex esophagitis and gastritis comprises jade powder, mineral contained in natural alkaline reduced water with pH 7.5-8.5 collected from a jade mine area, rosin, fructan, and plant-fermented enzyme liquid. The method for manufacturing the composition for alleviating and preventing reflex esophagitis and gastritis comprises the following steps: (a) mixing 3-7 parts by weight of jade powder having an average particle size of 100-300 mesh, 2-5 parts by weight of rosin, and 2-5 parts by weight of fructan with 50-100 parts by weight of natural alkaline reduced water with pH 7.5-8.5 collected from a jade mine area; (b) preparing a mixed melted product by stirring, mixing and heating a mixture, prepared in step (a), at 50-120°C and evaporating water; (c) quickly freezing the mixture without water and grinding the same; (d) spraying plant-fermented enzyme liquid to particles ground step (c); and (e) coating surfaces of the ground particles, prepared in step (d), with jade powder by inserting the ground particles into jade powder having an average particle size of 100-300 mesh.

Description

TECHNICAL FIELD The present invention relates to a composition for improving and preventing gastroesophageal reflux, gastroesophageal reflux disease and gastritis, and a method for preparing the same.

The present invention relates to a composition for improving and preventing reflux esophagitis and gastritis, and a method for producing the same.

Gastroesophageal reflux disease (GRD) has been a long-standing disease in western countries such as Europe and the United States, but has recently been increasing in Korea as well, due to Westernization of diet and lifestyle.

Heartburn is the most common symptom of reflux esophagitis. It usually refers to burning pain, burning, bitterness, or discomfort that seems to climb from the end of the stomach to the throat. This symptom can be spread to the back or neck and can be accompanied by dysphagia, which feels difficult to swallow.

Acid reflux is also an important symptom, which means that sickness, bitterness, or digestive food come back into your mouth. Sometimes the chest feels sick or hungry, and even when you do not eat, you may feel lumps in your neck. Repetitive pain or discomfort in the upper abdomen (concave chest) or chronic cough may also be a symptom of reflux esophagitis.

Heartburn in reflux esophagitis can occur any time of the day, but it is important to get worse after eating or lying down. It is often confused with heartburn, which is a more common symptom of functional dyspepsia or gastric ulcer or duodenal ulcer, which tends to increase the symptoms of the upper abdomen (the concave chest) before the meal is empty. Symptoms of reflux esophagitis and heartburn are very similar, but there is a slight difference in their location and time.

The biggest problem with this disease is that once it is symptomatic, it usually develops chronically and can reduce the quality of life. In other words, if the foreign body of the neck persists, the chronic cough, as well as bacteria act to decompose it, and it is difficult to take a good night's sleep because the bad breath is caused by bad breath and the symptom occurs mainly when lying down. In the case of reflux esophagitis, it is rare to leave, but it may develop into esophageal stricture or esophageal cancer. Therefore, if you feel any symptoms, it is important to find a hospital and get the correct diagnosis and treatment. In particular, drug therapy that simply suppresses gastric acid requires frequent treatment and dietary control because of the frequent recurrence of symptoms when the drug is discontinued or the dose is reduced.

In the treatment of reflux esophagitis, various drugs are used to inhibit the secretion of gastric acid. The most commonly prescribed drugs are hydrogen pump inhibitors such as omeprazole and rabeprazole, which reduce gastric acid secretion by more than 90% Even if the symptoms do not occur, it prevents esophageal lesions from progressing. Drugs that control the motility of the esophagus and lower esophageal sphincter are also used, and some of the above medications may be used together. Zantac and Mylanta are used to inhibit the secretion of gastric acid or put a film on the stomach acid, even if the stomach acid up the membrane of the esophagus is wrapped around the inflammation. The use of non-steroidal antiinflammatory drugs (NSAIDs), which are inflammatory diseases, can cause heartburn due to gastric acid secretion in the corresponding anti-inflammatory drugs. Aspirin, and ibuprofen.

If the symptoms are not improved by any of the above methods, surgery is recommended. Some complications have occurred and some have undergone surgery, but this is rare. Recently, laparoscopic surgery has been developed without opening the abdomen or the chest.

However, the chemo-synthetic drug treatment as described above may adversely affect the human body during long-term treatment, and the surgical treatment is a burden on the patient, and the effect is not excellent.

Therefore, there is a need to develop a method for improving and preventing reflux esophagitis by using natural ingredients besides chemical synthetic drug therapy and surgical therapy.

Patent Registration No. 10-1074839

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned disadvantages of the prior art,

Reflux esophagitis and gastritis can be effectively used without side effects by using natural alkaline reducing water, minerals, rosin, fructans and plant fermentation enzyme solution, which are collected from the natural ingredients such as jade powder and jade mines, And a composition for improving and preventing gastritis and a process for producing the same.

The present invention, in order to achieve the above object,

Rosin, fructan, and vegetable fermentation enzyme solution contained in natural alkaline reducing water of pH 7.5 to 8.5, which is collected from the jade powder and jade mine area,

(a) 3 to 7 parts by weight of jade powder having an average particle size of 100 to 300 mesh, 2 to 5 parts by weight of rosin and 2 to 5 parts by weight of fructan are mixed with a natural alkali having a pH of 7.5 to 8.5 To 50 to 100 parts by weight of reduced water;

(b) heating the mixture prepared in step (a) at 50 to 120 DEG C with stirring to prepare a mixed melt, and evaporating water;

(c) rapidly freezing and pulverizing the mixture from which the water has been removed;

(d) spraying a plant fermentation enzyme solution onto the ground particles in the step (c); And

(e) coating the surface of the ground particles prepared in the step (d) with a powder of jade powder having an average particle size of 100 to 300 mesh to improve reflux esophagitis and gastritis. ≪ / RTI >

The jade powder may be a nephrite powder.

The plant fermentation enzyme solution

(I) 1 to 10 parts by weight of an individual flower, 1 to 20 parts by weight of a bank, 1 to 10 parts by weight of spinach, 1 to 20 parts by weight of bellflower, 1 to 10 parts by weight of fennel, 1 to 20 parts by weight of fennel, 1 to 10 parts by weight of hymenoptera, 1 to 10 parts by weight of white chestnut mushroom, 1 to 20 parts by weight of cornstarch, 1 to 20 parts by weight of crumbs, 1 to 10 parts by weight of bean sprout flowers, 1 to 20 parts by weight And 1 to 20 parts by weight of graviola leaves;

(Ii) The herbaceous plant washed in step (i) is placed in a jar and 10 to 30 parts by weight of sugar capable of being fed to the microorganism is added in an amount of 10 to 30 parts by weight based on 100 parts by weight of the plant and one or more strains of lactic acid bacteria and yeast are inoculated Followed by fermentation at 20 to 40 ° C for 1 to 4 months;

 (Iii) squeezing out the fermented debris and squeezing for 1 to 10 months.

The composition for improving and preventing gastroesophageal reflux and gastritis may be a health functional food.

The present invention also relates to

(a) 3 to 7 parts by weight of jade powder having an average particle size of 100 to 300 mesh, 2 to 5 parts by weight of rosin and 2 to 5 parts by weight of fructan are mixed with a natural alkali having a pH of 7.5 to 8.5 To 50 to 100 parts by weight of reduced water;

(b) heating the mixture prepared in step (a) at 50 to 120 DEG C with stirring to prepare a mixed melt, and evaporating water;

(c) rapidly freezing and pulverizing the mixture from which the water has been removed;

(d) spraying a plant fermentation enzyme solution onto the ground particles in the step (c); And

(e) mixing the crushed particles prepared in the step (d) with a jade powder having an average particle size of 100 to 300 mesh, and coating the jade powder on the surface. The present invention also provides a method of preparing a composition for preventing such a disease.

Since the composition for improving and preventing gastroesophageal reflux and gastritis according to the present invention is prepared using a natural alkaline-reduced water having a pH of 7.5 to 8.5, rosin, fructan, and a plant fermentation enzyme, It can be taken easily by people with reflux esophagitis and gastritis, has no side effects, and has the effect of effectively improving and preventing reflux esophagitis and gastritis.

Fig. 1 shows a comparison of esophageal tissue damage in the acute reflux esophagitis model of Test Example 2,
Fig. 2 shows the histopathological examination results in the acute reflux esophagitis model of Test Example 2,
Fig. 3 shows the results of measuring the amount of iNOS expression in the esophageal tissue of Test Example 2,
Fig. 4 shows a comparison of gastric tissue damage in the acute gastritis model of Test Example 3,
FIG. 5 shows the results of histopathological examination in the acute gastritis model of Test Example 3,
Fig. 6 shows the results of measuring the amount of iNOS expression in the stomach tissue of Test Example 3,
Figs. 7 to 31 show the evaluation results (7 to 30), the esophageal photographing results, and the comprehensive evaluation results (31) of individual participants participating in the test as the clinical test results of Test Example 4.

Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings. Prior to the description of the present invention, terms and words used in the present specification and claims to be described below should not be construed as limited to ordinary or dictionary terms. Therefore, the embodiments described in this specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention and do not represent all the technical ideas of the present invention. Therefore, It is to be understood that equivalents and modifications are possible.

The present invention includes minerals, rosins, fructans and plant fermentation enzyme solutions contained in natural alkaline-reduced water having a pH of 7.5 to 8.5,

(a) 3 to 7 parts by weight of jade powder having an average particle size of 100 to 300 mesh, 2 to 5 parts by weight of rosin and 2 to 5 parts by weight of fructan are mixed with a natural alkali having a pH of 7.5 to 8.5 To 50 to 100 parts by weight of reduced water;

(b) heating the mixture prepared in step (a) at 50 to 120 DEG C with stirring to prepare a mixed melt, and evaporating water;

(c) rapidly freezing and pulverizing the mixture from which the water has been removed;

(d) spraying a plant fermentation enzyme solution onto the ground particles in the step (c); And

(e) coating the surface of the ground particles prepared in the step (d) with a powder of jade powder having an average particle size of 100 to 300 mesh to improve reflux esophagitis and gastritis. ≪ / RTI >

As the above-mentioned octane powder, a nephrite powder may be preferably used.

When the wound is scratched for several days with a nephrite powder, the main component of the nephrite is consistent with each element of the body, such as scars disappear and the skin is enriched. Is known to exert excellent efficacy.

The far-infrared rays inside the jade are effective for prevention of various adult diseases because the blood circulation of the body is energized and the heat is applied to the inside of the body. Further, the far-infrared rays function to maximize the activity of other components in the toothpaste.

According to Donguibogam, it makes the corn germs softer and makes the corn germs more radiant, expels waste in the body, eliminates the heat of the stomach and is effective in the digestive system. Also, when the corn is ingested, it becomes lighter and easier. It lubricates, helps the voice of the vocal cords, and is described as good for the throat.

It is preferable that the nephrite powder can be produced by using nephrite treated with remnants or wastes derived from mines or gemstones.

The average particle size of the nephrite powder may be 100 to 300 mesh.

The composition for improving and preventing gastroesophageal reflux and gastritis according to the present invention is characterized in that it is prepared using natural alkaline reducing water (also called 'corn oil') having a pH of 7.5 to 8.5, which is collected in the above-mentioned jade mine area. In other words, by adding the natural alkaline reducing water of pH 7.5 ~ 8.5 collected in the jade mine area and evaporating all pure water components, beneficial components such as minerals and minerals contained in the natural alkaline reducing water of pH 7.5-8.5 Can be included both in the reflux esophagitis and gastritis improving and preventing composition to be produced.

Groundwater produced in the Jade Mine area can be used as the natural alkaline water of pH 7.5 ~ 8.5 collected from the Jade mine area. The groundwater produced in the Jade Mine area is characterized by the mild nature of the water, its taste, and its clean nature. In addition, when people eat for a long time, the five-legged meat becomes rich, the waste in the body escapes, and the hair is not whitened. It is said that Chinese people have always been prone to jade because they have been directly influenced by the human body, stabilizing the nervous system and strengthening the functions of the five muscles.

Natural Alkali Reduced Water is water that is rarely known even in academia because it is rare in the world. In Korea or Japan, alkaline water is usually divided into alkaline water, which is produced by ionized water. Alkaline water is effective in chronic diarrhea, gastritis, gastric hyperplasia, dyspepsia, abnormal gastric fermentation, constipation, relieving fatigue, preventing colds, restoring appetite, hypertension, diabetes, allergies, rheumatism, relieving hangover, asthma and urticaria.

It is most preferred that the natural alkaline reducing water having a pH of 7.5 to 8.5 collected from the above-mentioned jade mine area is used for preparing the composition for improving and preventing gastroesophageal reflux and gastritis of the present invention. However, natural alkaline reducing water having a natural pH of 7.5 to 8.5 may be used in the present invention.

The pH of the natural alkali reduced water is more preferably 8.0 to 8.5.

The rosin acts as a medicinal component to bind the jute powder with the minerals extracted from the natural alkali reduced water. Rosin is also called 'rosin'.

Songjin is tasty and sweet, nature is warm and poisonous. It relaxes the mind, removes the heat, governs the wind and the dead skin, and treats all the males, head pals, white fur, and spot fur, ) And the taste of the teeth, and attaches it to all the windows, the skin is born, the pain stops, and it kills the insect. It is called a song or song. It is better to take things that come out in June than to take hard ones or to be better than the ones that have been taken from the moon, and that is the same as the discipline incense. <Dongbu Bang>

Songjin has been suffering from bruise, bruise, fainting, bruise, bruise, fatigue, toothache, flavor, chidamen, calvaine, insomnia, ringworm, genomes, pulmonary tuberculosis, pneumonia, It was used to heal the back.

Songjin is a pharmacologic effect that immediately causes stigmata, petechiae, inflammation, scurvy, scalding, insecting, brightening eyes, eliminating rotten flesh and bringing out new life.

The fructan is a dietary fiber and serves to bind the minerals extracted from the natural alkali reduced water with the jade powder. Fructans are polysaccharides composed of fructose. Fructan is a polymer composed of fructose, and when ingested, it functions to prevent obesity, promote mineral absorption, and stimulate the growth of lactic acid bacteria in the intestines. It is a biomolecule composed of thousands to tens of thousands of fructose, a natural substance found in plants and microorganisms. It is a biopolymer of our representative traditional food such as chungkukjang, amber, onion, and garlic which are known to be good for health, and plants such as barley, cactus, .

Fructans consist mainly of fructose units and fructose-fructose bonds consist of carbohydrate chains that make up the majority of the bonds. Fructans are usually produced as polydisperse carbohydrates. They are present in plants, but they can originate from bacterial activity and can also be enzymatically synthesized.

Fructans are well known compounds including levan and inulin carbohydrates. Levan is a D-fructose consisting of a fructose unit chain predominantly linked to each other by β (2-6) bonds. Inulin is made up of difunctional units, but they are also linked to each other by β (2-1) bonds. Most inulin is terminated in one glucose unit.

Fructans, particularly inulin, are also known to have beneficial effects on lipid metabolism, including lowering blood cholesterol and serum triglycerides and increasing HDL / LDL ratios.

P.D. Cooper et al., Molecul. Immunol., 23 (8), 895, (1986)) describes another pathway of gamma-inulin-dependent complement activation and that other active pathways in the complement pathway have potential non-specific antitumor effects . In addition, fructans, in particular inulin, have been described as potentially for the prevention and treatment of cancer. P. D. Cooper et al., Molecul. Immunol., 23 (8), 895, (1986)], it has been reported that gamma-inulin administered intraperitoneally can prolong the survival of melanoma-bearing mice.

In the present invention, the plant fermentation enzyme solution

(I) 1 to 10 parts by weight of an individual flower, 1 to 20 parts by weight of a bank, 1 to 10 parts by weight of spinach, 1 to 20 parts by weight of bellflower, 1 to 10 parts by weight of fennel, 1 to 20 parts by weight of fennel, 1 to 10 parts by weight of hymenoptera, 1 to 10 parts by weight of white chestnut mushroom, 1 to 20 parts by weight of cornstarch, 1 to 20 parts by weight of crumbs, 1 to 10 parts by weight of bean sprout flowers, 1 to 20 parts by weight And 1 to 20 parts by weight of graviola leaves;

(Ii) The herbaceous plant washed in step (i) is placed in a jar and 10 to 30 parts by weight of sugar capable of being fed to the microorganism is added in an amount of 10 to 30 parts by weight based on 100 parts by weight of the plant and one or more strains of lactic acid bacteria and yeast are inoculated Followed by fermentation at 20 to 40 ° C for 1 to 4 months;

 (Iii) squeezing out the fermented debris and squeezing for 1 to 10 months.

The individual flowers are used by the private to supplement the stomach, to strengthen the stomach, and to improve the yang. The taste is sweet, a little bit worn, and the quality is plain. Lengthen the lungs and stomach, and increase the amount of essence. After suffering from sickness, people who are frail, weak children or elderly people are said to be strong. Individual flowers are similar in effect to ginseng, and there are no side effects that can occur when ginseng is eaten.

Individual flowers are mental fatigue, self-indulgent symptoms, amnesia, insomnia, no appetite, and when I drink inside my mouth, when my chest is throbbing.

It is a medicinal plant used as a herbal medicine in Oriental medicine and contains various physiologically active substances. The bark of Melia azedarach Linne var. Japonica Makino, a deciduous tree belonging to Meliaceae, is a bark or muscle in Oriental medicine. It is caused by rubella, malaria, leprosy, leprosy, fever, skin eczema, diuretic, , Gynecological diseases and vaginitis. It has also been reported that pharmacological actions are effective in insecticidal action, antipyretic action, tapeworm or concretion elimination (antiparasitic effect) and trichomonas vaginitis (antigenic effect).

The fungus is a universal herb that is efficacious in diseases such as diabetes mellitus, otitis media, hepatitis, bronchitis gall stones, kidney stones, asthma and ischemic diseases. We call the gold coins a long cherry paste. It grows everywhere in our country, but it is common in the sunny and sunny areas.

The perennial plant grows about 5 ~ 25cm tall and the leaves face in kidney shape. The flowers are light purple and bloom in June-August, and hard berries ripen in September. There are a lot of honey in the flowers and a lot of bees gather. There are other names, such as the bow,

There is an action that makes urine look better, heals jaundice and heals body swelling. It also has the effect of lowering heat, poisoning, cutting inflammation and eliminating eosinophils. It also has the effect of dissolving bladder, urethra, and gall stones, and also has the effect of stopping coughing and shaving sputum. Eczema, boils and skin diseases, such as rashing things that are better if you stick.

The madder is called the nerve root of the roots and is used in the private for the treatment of neuralgia, arthritis, edema jaundice. It also has a remarkable effect in dissolving kidney and bladder stones.

It grows frequently in the mountains, villages and fences of various parts of Korea. It grows about 2m in length, the stem is square, and the leaves turn back to the heart. The stem is empty but the root is plump and reddish. Light yellow flowers bloom from July to August, and black and round fruit is formed in September.

Madder roots have an excellent effect in dissolving stones in the kidneys and bladder. The water of the madder roots slowly inflates the kidney and bladder stones and melts them away. The ingredient, rubeitrin acid in the root of the madder, acidifies the urine to dissolve the calcium phosphate stones, and the red pigment ingredient dissolves the stones.

It is a medicinal product of blood, blood and blood. It is a good remedy for the menstrual irregularities, uterine bleeding, infantile hemorrhage, and endometritis. It is also used for jaundice, swelling, bruise, and chronic bronchitis. It also has an effect on arthritis and diuretic action, which can be used to urinate poorly and has a certain effect on stopping coughing.

It is a perennial plant, with anemone and tannin in its roots. Fresh roots contain protoanomonin in the form of two molecules in the air to become anemonin. Pulsatilla koreana (Pulsatilla koreana) is a perennial herb that grows about 40cm in height, and grows in mountainous, dry dogs. The flowers bloom in April-May with red purple, the fruit is covered with white fur, and it is used for headache, edema, dysentery, heart disease, schizophrenia and gastritis. It is known that there is a mystifying effect in treating brain diseases in particular. It is possible to fix various kinds of cancer including brain tumor by using lawful roots well.

The above-mentioned Tremella fuciformis grows in various hardwood dead trees or branches in summer and autumn. Fruiting body is 3 ~ 8cm in diameter, 2 ~ 5cm in height, lumpy shape with irregular wave shape. The surface is pure white, smooth, and the fascial layer develops throughout the surface. The tissue is translucent gelatinous. It is medicinal mushroom which has immunity enhancement, cholesterol lowering, blood sugar lowering, antioxidant effect by edible mushroom, and it is known that it is helpful in various diseases such as respiratory diseases, gastritis, hypertension and menstrual irregularity. The main components include vitamins B1, B2, D and niacin, mannitol, glycerol, glucose, trehalose, hemicellulose, chitin, pectin, (pectin), luconoxylomannan, lignin, and the like are known.

Beard Phalaenopsis is a medicinal plant used as herbal medicine in Oriental medicine and contains various physiologically active substances. Beard spiny flowers are called semi-mature, semi-irregular, or hasty colors. They are found in central and southern Jeju-do in Korea and live in ditches, ditches, and wetlands of rice paddies. Beard spiny flowers have traditionally been used for the treatment of diarrhea, inflammation and detoxification and have been used as medicines for the treatment of asthma, dyspnea, pertussis, malignant boils, eczema, and traumatic hemorrhage.

Akebiae quinata Decne is a deciduous vine plant belonging to the genus Lepidoptera, and grows throughout the country. In one tree, female flowers and male flowers are used separately for different ornamental purposes. In addition, young leaves and fruit are used for edible, root and branch medicinal purposes. Specifically, the sprouts and young stems were eaten as herbs, and the flowers were dried and used instead of perfume. Stem boiled water was used as a yellow or yellowish dye, and was especially important for medicinal use because it has efficacy for anti-inflammatory diuretics, urinalysis, urethritis, sore throat, and psychotropic drugs. Roots and bark are effective for urination disorders, and fruits are effective for diuretics, paralysis, and arthritis.

The lactic acid bacterium which can be used for the production of the plant fermentation enzyme solution is selected from the group consisting of Lactobacillus sp., Streptococcus sp., Pediococcus sp., Leuconostoc sp. Lactic acid bacteria Leuconostoc sp.), Bifidobacterium sp. (Bifidobacterium sp.), And the like.

Examples of the yeast include Saccharomyces sereviciae, Saccharomyces rouxii, Saccharomyces oviformis, Saccharomyces steineri, Saccharomyces cerevisiae, ) May be used.

The saccharide may be a mixture of honey and sugar at a weight ratio of 1: 3: 1 ~ 3.

The composition for improving and preventing gastroesophageal reflux and gastritis according to the present invention may be used as a health functional food composition itself, and may be prepared in various forms by further adding a carrier, excipient or diluent. Examples of the carrier, excipient and diluent include crystalline glucose, trehalose, xanthan gum, gum arabic, guar gum, skim milk powder, maltodextrin, gelatin, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, The present invention relates to a method for producing a microcapsule comprising a microcapsule containing at least one selected from the group consisting of acacia rubber, alginate, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, .

The health functional food composition may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, solutions, expansions, tea, jellies, aerosols, drinks and the like.

The health functional food composition may contain various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate etc.), pectic acid and its salts, alginic acid and its salts , Organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.

The present invention also relates to

(a) 3 to 7 parts by weight of jade powder having an average particle size of 100 to 300 mesh, 2 to 5 parts by weight of rosin and 2 to 5 parts by weight of fructan are mixed with a natural alkali having a pH of 7.5 to 8.5 To 50 to 100 parts by weight of reduced water;

(b) heating the mixture prepared in step (a) at 50 to 120 DEG C with stirring to prepare a mixed melt, and evaporating water;

(c) rapidly freezing and pulverizing the mixture from which the water has been removed;

(d) spraying a plant fermentation enzyme solution onto the ground particles in the step (c); And

(e) mixing the crushed particles prepared in the step (d) with a jade powder having an average particle size of 100 to 300 mesh, and coating the jade powder on the surface. And a method for preparing the composition.

The above-mentioned contents in the composition for improving and preventing gastroesophageal reflux and gastritis can be applied to the above-described preparation method. Therefore, duplicate content is omitted.

In the present invention, the term " improvement "is meant to include alleviation, prevention or treatment of reflux esophagitis and gastritis symptom relief and prevention by administering, ingesting or applying to a subject having reflux esophagitis.

delete

The dose of the composition for improving and preventing gastroesophageal reflux and gastritis of the present invention is not particularly limited, but it is preferable that the daily dose is not more than 20 g, and 1 to 5 g of the composition is administered at a time with or without water desirable.

Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited to the following examples.

Manufacturing example  1: Plant fermentation Enzyme solution  Produce

5 parts by weight of individual flowers, 10 parts by weight of bank, 5 parts by weight of bellflower, 10 parts by weight of bellflower, 5 parts by weight of fennel, 10 parts by weight of fennel, 5 parts by weight of madder, 5 parts by weight of horticulture, 10 parts by weight of corn, 10 parts by weight of beeswax, 5 parts by weight of bean sprouts and 10 parts by weight of graviola leaves and 10 parts by weight of graviola leaves were placed in a dried jar and honey and brown sugar were mixed in a ratio of 1: 1 was added in an amount of 20 parts by weight based on 100 parts by weight of the herbaceous plant. Then, Streptococcus sp. Strain was inoculated, and the jar was sealed to allow air to pass through and stored in the shade. The herb plant was inverted once a month and fermented for 3 months. Then, the fermented residue was squeezed out and sieved for 9 months to prepare a plant fermentation enzyme solution.

Example  1: composition for improving and preventing reflux esophagitis and gastritis

150 g of nephrite powder having an average particle size of 200 mesh in a cauldron, 150 g of rosin, and 150 g of fructan were taken as a natural alkaline reducing water having a pH of 7.5 to 8.5, which was collected from Jade mine area and manufactured by Daeil Mining Co., . Next, while the cauldron was heated to 100 DEG C, the mixture was stirred and the water was evaporated. The water-evaporated mixture was solidified by rapid cooling. After the solid composition prepared above was pulverized, 500 ml of the plant fermentation enzyme solution prepared in Preparation Example 1 was sprayed on the pulverized solid composition and dried. Next, the dried pulverized solid composition was put into a nephrite powder having an average particle size of 200 mesh and rolled to prepare a composition for improving and preventing gastroesophageal reflux and gastritis, the surface of which was coated with nephrite powder.

Test Example  1: Assessment of efficacy of compositions for improving and preventing gastroesophageal reflux and gastritis

10 male and 10 female subjects with reflux esophagitis were selected as the experimental group. The composition for the improvement and prevention of gastroesophageal reflux and gastritis prepared in Example 1 was administered at intervals of 2 to 3 hours The degree of improvement of reflux esophagitis was evaluated at 10 days, 20 days and 30 days after administration, and the results are shown in Table 1 below.

<Evaluation Criteria>

◎: 80% improved

○: 50% improved

△: 20% improvement

X: No change

××: worse

Subject After 10 days After 20 days After 30 days Male 1 △ ○ ◎ Male 2 △ △ ○ Male 3 △ ○ ◎ Male 4 △ △ ○ Male 5 △ ○ ◎ Woman 1 △ ○ ◎ Woman 2 △ △ ○ Woman 3 △ ○ ○ Women 4 △ ○ ○ Women 5 △ ○ ◎

From the results shown in the above Table 1, it was confirmed that the subjects receiving the reflux esophagitis and compositions for improving and preventing gastritis according to the present invention had excellent reflux esophagitis and gastritis improvement. No side effects were observed at all.

Test Example  2: Acute in animal models Reflux esophagitis  Improvement effect experiment

Using alkaline reducing water (Doctor M, SungMin KOREA Co., Ltd., pH 7.5 ~ 8.5) collected from the jade mine area containing specific minerals, the alkaline mineral water for 6 - week - old Rat, an animal model of reflux esophagitis, Clinical trials were carried out to verify the effects of treatment on symptom improvement.

The reflux esophagitis animal model was used for the experiment after 1 week of adaptation in the environment of 22 ± 2 ℃, humidity 40 ± 5% and light-night of 12 hours with 6-week-old Rat. Respectively. Treatment groups were divided into two groups: Group 1 (Normal, n = 6), Group 2 (RE Control, n = 6), Group 3 (Doctor M), and n = 6. Groups 1 and 2 received tap water for 7 days Group 3 supplied Dr.M. Group 1 and Group 2 were treated with oral tap water and Doctor M, respectively. After 1 hour and 30 minutes, they were sutured after pyloric and avascular ligation. Five hours after the operation, the esophagus was lyophilized for analysis.

Rat weight Rat weight Rat weight Normal-1 191 RE control-1 202 Doctor M-1 201 Normal-2 204 RE control-2 209 Doctor M-2 203 Normal-3 198 RE control-3 193 Doctor M-3 203 Normal-4 199 RE control-4 192 Doctor M-4 189 Normal-5 194 RE control-5 199 Doctor M-5 194 Normal-6 212 RE control-6 194 Doctor M-6 193

(Unit: g)

(1) Measurement of damage degree

After 5 hours of operation, the esophagus was removed and the esophagus was cut longitudinally using surgical scissors. The cut esophagus was rinsed with an appropriate amount of 0.9% NaCl and developed using an optical digital camera (Nicon). The damaged esophageal mucosa was measured using the image J program. In control group (RE control), which caused reflux esophagitis by feeding tap water before operation, esophageal mucosa was chemically damaged by stomach acid, resulting in redness, redness and inflammation. The area ratio of damage area was 47.1%. It was confirmed that the damage was effectively reduced in 9.21% of the experimental group which caused the reflux esophagitis after the 7-day administration and 1-oral administration of the doctor M (Fig. 1).

(2) Histopathological examination

H-E staining was performed to confirm the anti-inflammatory effect on the development of reflux esophagitis by histopathological examination. At the end of the experiment, the animals were anesthetized with anesthesia and the tissues were extracted. The tissues were fixed with 10% neutral buffered formalin, and then embedded in paraffin through dehydration and transparency with ethanol and xylene. The tissue was stained with hematoxylin and eosin, and the stained tissue was observed with an optical microscope (Leica DM 2500). The tissue was photographed and analyzed with an image analysis program (i-solution DT) In the non-treated group, normal mucosal structure was observed, but in the case of reflux esophagitis, a wide range of esophageal epithelium disappeared. On the other hand, the doctor M administration group maintained the tissue structure of the esophageal epithelium similar to normal tissue (Fig. 2).

(3) Inflammation-inducing protein iNOS  Measurement of expression level

Expression levels were measured by Western blot using isolated esophageal tissue. In the isolated esophageal tissue, the cytoplasm was isolated using Buffer A, and the loading sample was prepared by adding Blue-buffer after quantification of the protein through BSA. Each sample was subjected to SDS-polyacrylamide gel electrophoresis at 120V using running gel and stacking gel. For transfer, membranes were transferred using a transbuffer for 60 minutes and blocked with 5% skim-milk solution. The iNOS and b-actin primary antibodies were reacted at 4 ° C for 18 hours or more, washed 3 times with 1X PBS, reacted with the secondary antibody for 2 hours at room temperature, and washed with 1X PBS. The washed membranes were mixed with ECL detection kit, coloring reagents Ⅰ and Ⅱ in a ratio of 1: 1, and observed using a Chemidoc system (Bio-Rad). As a result, it was confirmed that when Dr. M was treated, the amount of iNOS expression, which is an inflammation inducer, was reduced by 53% as compared with the RE control treatment (FIG. 3).

Test Example  3: Experiments on the improvement of acute gastritis in animal models

The reflux esophagus animal model was used for post-adaptation experiments at 22 ± 2 ° C, humidity 40 ± 5% and light-night 12-h after receiving 6-week-old mice. Respectively. Group 1 (Normal, n = 6), Group 2 (Control, n = 6), Group 3 (Doctor M) and n = 6) 3 supplied Dr.M. Groups 2 and 3 were orally administered with tap water and Doctor M, respectively, and oral administration of 300 mM HCl + 60% ethanol for gastritis 1 hour later. One hour later, for cervical tissue analysis, cervical dislocation was performed, followed by laparotomy.

Rat weight Rat weight Rat weight Normal-1 30 control-1 31 Doctor M-1 30 Normal-2 31 control-2 30 Doctor M-2 29 Normal-3 33 control-3 30 Doctor M-3 29 Normal-4 25 control-4 28 Doctor M-4 30 Normal-5 25 control-5 31 Doctor M-5 32 Normal-6 31 control-6 30 Doctor M-6 29

(Unit: g)

(1) Measurement of damage degree

The stomach tissue was isolated 1 hour after gastritis induction. The inside of the esophagus was cleaned with an appropriate amount of 0.9% NaCl and developed using an optical digital camera (Nicon). As a result, it was confirmed that tissue damage was alleviated when Doctor M was fed and gastritis was induced compared to the treatment group in which general water was fed and then gastritis was induced (FIG. 4).

(2) Histopathological examination

H-E staining was performed to confirm the anti-inflammatory effect of gastritis by histopathological examination. At the end of the experiment, the animals were anesthetized with anesthesia and the tissues were extracted. The tissues were fixed with 10% neutral buffered formalin, and then embedded in paraffin through dehydration and transparency with ethanol and xylene. The tissue was stained with hematoxylin and eosin at 5 μm thickness. The stained tissue was observed with an optical microscope (Leica DM 2500) and analyzed with an image analysis program (i-solution DT). In the gastritis group, normal mucous membrane structure was observed, however, inflammatory cell deposition and severe mucosal epithelial cell disappeared in the mucosa and submucosa in the gastritis group treated with HCl / EtOH. On the other hand, it was confirmed that the induction of inflammation by HCl / EtOH treatment was inhibited in the M-treated group, and the morphological inflammatory cell deposition or mucosal epithelial cell disappeared was observed in the tissue structure close to the normal tissue as compared with the HCl / EtOH alone treatment group 5).

(3) Inflammation-inducing protein iNOS  Measurement of expression level

The expression level was measured by Western blot using the isolated stomach tissue. After separating the cytoplasm using Buffer A in the isolated stomach, the protein was quantitated by BSA and then added with Blue-buffer to prepare a loading sample. Each sample was subjected to SDS-polyacrylamide gel electrophoresis at 120V using running gel and stacking gel. For transfer, membranes were transferred using a transbuffer for 60 minutes and blocked with 5% skim-milk solution. The iNOS and b-actin primary antibodies were reacted at 4 ° C for 18 hours or more, washed 3 times with 1X PBS, reacted with the secondary antibody for 2 hours at room temperature, and washed with 1X PBS. The washed membranes were mixed with ECL detection kit, coloring reagents Ⅰ and Ⅱ in a ratio of 1: 1, and observed using a Chemidoc system (Bio-Rad). As a result, it was confirmed that the amount of iNOS expression, which is an inflammation inducer, was inhibited by 14% when Drug M was fed and gastritis was induced as compared to the treatment which induced gastritis after feeding with ordinary water (Fig. 6).

Test Example  4: Reflux esophagitis  Improvement clinical trial

(1) Outline of experiment

The purpose of this study was to evaluate the effect of alkaline mineral water on the improvement of symptoms of reflux esophagitis by drinking the natural alkaline reducing water (Doctor M) at pH 7.5 ~ 8.5 collected from the jade mine area containing specific minerals in patients with reflux esophagitis The test was conducted.

(2) Clinical test method

From March 2017 to July 2017, a clinical examination was conducted in a patient who had been diagnosed with gastroesophageal reflux disease (gastroscopy) at a hospital (Park, Gapjung Medical Center, Bupyeong-gu, Incheon).

a. A sufficient explanation for the pre-experiment clinical study was given and each participant received the clinical trial consent.

b. Natural alkaline-reduced water (Dr. M), pH 7.5-8.5, collected in the jade mine area of 15ml / kg per adult per day was drunk.

c. The duration of drinking was 2 months, and the duration was adjusted according to the patient 's condition and clinical response.

d. During the study period, the patients visited the hospital periodically and evaluated their progress.

e. It is the principle to prohibit the use of other medications during the clinical trial period, and the drug may be paid if necessary for the convenience of the patient.

f. If you have any other medication you are taking and keep your usual lifestyle, take it as is.

g. Endoscopy was performed before and 2 to 4 months after the operation to evaluate progress.

h. A questionnaire was made to check the patients' condition and after 2 months, the results were summarized and compared with those before the experiment.

(3) Clinical test results

The results of individual clinical studies are shown in FIGS. 7 to 30, and the comprehensive clinical results are shown in FIG.

 As can be seen from FIGS. 7 to 30 and FIG. 31, it was confirmed that the natural alkali reduced water (Doctor M) having a pH of 7.5 to 8.5, which was collected in the jade mine area containing special minerals, showed a very excellent effect on the reflux esophagitis have. That is, 93% of the participants in the clinical trial were evaluated as effective, and 72% of the participants were evaluated as having a very good effect.

In particular, even when compared with existing drugs, 78% of the participants in the study were evaluated to be equivalent to the drug, and 57% were evaluated as superior to the drug.

Claims (5)

Rosin, fructan, and vegetable fermentation enzyme solution contained in natural alkaline reducing water of pH 7.5 to 8.5, which is collected from the jade powder and jade mine area,
(a) 3 to 7 parts by weight of jade powder having an average particle size of 100 to 300 mesh, 2 to 5 parts by weight of rosin and 2 to 5 parts by weight of fructan are mixed with a natural alkali having a pH of 7.5 to 8.5 To 50 to 100 parts by weight of reduced water;
(b) heating the mixture prepared in step (a) at 50 to 120 DEG C with stirring to prepare a mixed melt, and evaporating water;
(c) rapidly freezing and pulverizing the mixture from which the water has been removed;
(d) spraying a plant fermentation enzyme solution onto the ground particles in the step (c); And
(e) coating the surface of the ground particles prepared in the step (d) with a powder of jade powder having an average particle size of 100 to 300 mesh, thereby improving and preventing reflux esophagitis and gastritis. / RTI &gt; The method according to claim 1,
Wherein the jade powder is a nephrite powder. The method according to claim 1,
The plant fermentation enzyme solution
(I) 1 to 10 parts by weight of an individual flower, 1 to 20 parts by weight of a bank, 1 to 10 parts by weight of spinach, 1 to 20 parts by weight of bellflower, 1 to 10 parts by weight of fennel, 1 to 20 parts by weight of fennel, 1 to 10 parts by weight of hymenoptera, 1 to 10 parts by weight of white chestnut mushroom, 1 to 20 parts by weight of cornstarch, 1 to 20 parts by weight of crumbs, 1 to 10 parts by weight of bean sprout flowers, 1 to 20 parts by weight And 1 to 20 parts by weight of graviola leaves;
(Ii) The herbaceous plant washed in step (i) is placed in a jar and 10 to 30 parts by weight of sugar capable of being fed to the microorganism is added in an amount of 10 to 30 parts by weight based on 100 parts by weight of the plant, and one or more strains of lactic acid bacteria and yeast are inoculated Followed by fermentation at 20 to 40 ° C for 1 to 4 months;
(Iii) squeezing out the fermented sludge, and aging the sludge for 1 to 10 months. The composition for improving and preventing gastritis of reflux esophagitis and gastritis. The method according to claim 1,
Wherein the composition for improving and preventing gastroesophageal reflux and gastritis is a health functional food. (a) 3 to 7 parts by weight of a jade powder having an average particle size of 100 to 300 mesh, 2 to 5 parts by weight of rosin and 2 to 5 parts by weight of fructan are mixed with a natural alkali having a pH of 7.5 to 8.5 To 50 to 100 parts by weight of reduced water;
(b) heating the mixture prepared in step (a) at 50 to 120 DEG C with stirring to prepare a mixed melt, and evaporating water;
(c) rapidly freezing and pulverizing the mixture from which the water has been removed;
(d) spraying a plant fermentation enzyme solution onto the ground particles in the step (c); And
(e) mixing the crushed particles prepared in the step (d) with a jade powder having an average particle size of 100 to 300 mesh, and coating the jade powder on the surface. Gt;

Images