KR101851132B1 - Composition for enhancing plant growth comprising heneicosane as an effective agent - Google Patents
Composition for enhancing plant growth comprising heneicosane as an effective agent Download PDFInfo
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- KR101851132B1 KR101851132B1 KR1020160080233A KR20160080233A KR101851132B1 KR 101851132 B1 KR101851132 B1 KR 101851132B1 KR 1020160080233 A KR1020160080233 A KR 1020160080233A KR 20160080233 A KR20160080233 A KR 20160080233A KR 101851132 B1 KR101851132 B1 KR 101851132B1
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- A—HUMAN NECESSITIES
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
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Abstract
본 발명은 하이네코사인을 유효성분으로 포함하는 조성물에 관한 것으로, 본 발명을 식물에 적용하면 식물의 생장을 촉진할 수 있어, 다양한 식물 생장 촉진용제제로 활용될 수 있다. The present invention relates to a composition comprising henexane as an active ingredient, and the present invention can be applied to plants to promote the growth of plants, and thus can be utilized as various plant growth promoting agents.
Description
본 발명은 식물 생장 촉진용 조성물 및 이를 식물에 적용하여 식물의 생장을 촉진시키는 방법에 관한 것이다. The present invention relates to a composition for promoting plant growth and a method for promoting plant growth by applying the composition to plants.
통상적으로 식물 생장 촉진제는 작목하고자 하는 식물, 특용작물, 화훼류 또는 과수의 성장을 촉진시키기 위한 것으로써, 다양한 종류의 식물 생장 촉진제가 시중에 출시되고 있으며, 이러한 식물 생장 촉진제는 식물 체내에서 생성되는 천연의 식물 호르몬 또는 이와 유사한 화학구조 및 생리적 기능 지닌 화학물질이다.Generally, plant growth promoters are used for promoting the growth of plants, special crops, flowers or fruit trees to be planted, and a variety of plant growth promoters are on the market. Such plant growth promoters are natural products Of plant hormones or chemicals with similar chemical structures and physiological functions.
식물의 생육을 촉진시키는 성분으로는 식물의 생장촉진, 발근, 착과촉진, 탈리형성을 억제하는 기능이 있는 옥신류와, 생장촉진, 착과촉진, 웅성불임단위결과 유도, 화아분화 촉진, 휴면타파, 발아 등의 생육 등을 촉진시키는 기능이 있는 지베렐린, 세포분열촉진, 휴면타파, 기관분화 유도 등을 촉진하는 기능이 있는 시토키닌 등 다양한 성분들이 있으며, 식물 생장 촉진제의 수요가 증가함에 따라 소비자들의 욕구를 충족시키기 위해 다양한 종류의 식물 생장 촉진제들이 연구 개발되고 있다.Examples of the components that promote plant growth include auxins having a function of promoting plant growth, rooting, fusing, promoting and desizing, and promoting growth, fusing and promoting, inducing male sterility unit results, promoting plant differentiation, Gibberellin which has the function of promoting the growth of germination and the like, cytokinin which has the function of promoting cell division, dormancy breakage, induction of the differentiation of the organ, etc., and as the demand of the plant growth promoter increases, Various kinds of plant growth promoters are being researched and developed to meet these needs.
한편, 하이네코사인(heneicosane)은 C21H44의 화학식으로 나타나는 장쇄 포화된 지방족 탄화수소이다. 하이네코사인의 분자량은 296.57 g/mol이고, 끓는점은 673.7 °F (at 760 mmHg), 녹는점은 104.9 °F인 휘발성의 유기 화합물이다. 하이네코사인에 대해서는 뎅기 벡터 이집트 숲모기(dengue vector Aedes aegypti)의 산란 반응 유도제로 보고되어 왔다(Y.H. Elshiekh, A.M.A. Mona, Am. J. Res. Comm. 3 (2015) 58-67.). 또한, 하이네코사인은 약용 식물인 Pulicaria crispa 및 성게( Temnopleurus alexandri)의 항미생물 활성 발생 요소로 보고된 바 있다(B. Uma, R. Parvathavarthini, Int. J. PharmTech Res. 2 (2010) 1677-1680.).On the other hand, heneicosane is a long-chain saturated aliphatic hydrocarbon represented by the formula C 21 H 44 . Heine cosine has a molecular weight of 296.57 g / mol, a boiling point of 673.7 ° F (at 760 mmHg) and a melting point of 104.9 ° F. Heinecosene has been reported as a scattering reaction inducer of dengue vector Aedes aegypti (YH Elshiekh, AMA Mona, Am. J. Res. Comm. 3 (2015) 58-67). In addition, Heinecosine is a medicinal plant, Pulicaria crispa And Sea urchin ( Temnopleurus has been reported with the anti-microbial activity of the generating element bar alexandri). (B. Uma, R. Parvathavarthini, Int. J. PharmTech Res. 2 (2010) 1677-1680).
본 발명이 해결하고자 하는 과제는 하이네코사인을 유효성분으로 포함하는 식물 생장 촉진용 조성물을 제공하는데 있다. It is an object of the present invention to provide a plant growth promoting composition comprising a herbicide as an active ingredient.
또한, 본 발명이 해결하고자 하는 과제는 하이네코사인을 유효성분으로 포함하는 식물 생장 촉진용 조성물을 식물에 적용하여 식물의 생장을 촉진시키는 방법을 제공하는데 있다. Another object of the present invention is to provide a method for promoting plant growth by applying a plant growth promoting composition comprising henexacin as an effective ingredient to plants.
상술한 과제를 해결하기 위하여, 본 발명은 하이네코사인을 유효성분으로 포함하는 식물 생장 촉진용 조성물을 제공한다. 또한, 하이네코사인을 유효성분으로 포함하는 식물 생장 촉진용 조성물을 식물에 적용하여 식물의 생장을 촉진시키는 방법을 제공한다. In order to solve the above-mentioned problems, the present invention provides a plant growth promoting composition comprising henexacin as an active ingredient. Also provided is a method for promoting plant growth by applying a plant growth promoting composition containing henexacin as an effective ingredient to a plant.
본 발명자들은 하이네코사인을 담배 식물에 적용한 결과 담배 식물의 생중량이 현저히 상승함을 확인하고, 하이네코사인을 식물에 적용하면 생장 촉진에 효과적임을 발견하고 본 발명을 완성하였다. The present inventors have found that the application of heneicosane to tobacco plants significantly increases the fresh weight of tobacco plants and that heinececone is effective in accelerating the growth when applied to plants, and completed the present invention.
[하이네코사인][Heine Cosine]
본 발명에 따른 하이네코사인은 천연물질로부터 분리정제하여 사용할 수도 있는바, 예를 들어 콜타르(coal tars) 또는 화식 유기물(fossil organic matter)에서 분리정제할 수도 있고, 당업계 공지된 방법으로 화학적으로 합성하여 사용할 수도 있으며, 상업적으로 유통되고 있는 화합물을 이용할 수도 있다. The herpes simplex according to the present invention may be isolated and purified from natural materials, for example, be separated and purified from coal tars or fossil organic matter, chemically synthesized by a method known in the art Or a commercially available compound may be used.
본 발명에 있어서, 용어 '하이네코사인'은 화학식 C21H44의 화합물과 이의 염을 통칭하는 것으로 사용된다. 본 발명에 있어서, 상기 용어 '염'은 표적(예를 들어, 표적 식물)이 생리학적으로 수인 가능한 본 발명의 화합물의 모든 (예컨대, 산 또는 염기와 반응하여 얻은) 염을 의미한다. 본 발명 화합물의 염은 유기 또는 무기산 및 염기로부터 유도될 수 있다. 산의 예로서는 염산, 브롬산, 황산, 질산, 과염소산, 푸마르산, 말레산, 인산, 글리콜산, 락트산, 살리실산, 숙신산, 톨루엔-p-황산, 타르타르산, 아세트산, 시트르산, 메탄설폰산, 에탄설폰산, 포름산, 벤조산, 말론산, 설폰산, 나프탈렌-2-설폰산, 벤젠설폰산 등이 포함되나 이에 한정되지 않는다. 그 자체로는 허용할 수 없지만 옥살산 등의 그 밖의 산 또한 본 발명의 화합물 및 이들의 첨가염을 획득하기 위한 중간체로서 유용한 염의 제조시 사용될 수 있다. 염기의 예에는 알칼리 금속(예컨대, 나트륨) 수산화물, 알칼리 토금속(예컨대, 마그네슘) 수산화물, 및 암모니아를 포함하나 이에 한정되지 않는다. 염의 예에는 아세테이트, 아디페이트, 알기네이트, 아스파레이트, 벤조에이트, 벤젠설포네이트, 바이설페이트, 부티레이트, 시트레이트, 캄포레이트, 캄포설포네이트, 시클로펜탄프로피오네이트, 디글루코네이트, 도데실설페이트, 에탄설포네이트, 푸마레이트, 플루코헵타노에이트, 글리세로포스페이트, 헤미설페이트, 헵타노에이트, 헥사노에이트, 클로라이드, 브로마이드, 아이오다이드, 2-하이드록시에탄설포네이트, 락테이트, 말레이트, 메탄설포네이트, 2-나프탈렌설포네이트, 니코티네이트, 옥살렌이트, 파모에이트, 펙티네이트, 퍼설페이트, 페닐프로피오네이트, 피크레이트, 피발레이트, 프로피오네이트, 숙시네이트, 타르트레이트, 티오시아네이트, 토실레이트, 언데카노에이트 및 이와 유사한 것을 포함하나 이에 한정되지 않는다. 그 밖의 염의 예에는 Na+, NH4+, 및 NW4+ (여기서 W는 Cl-C4의 알킬기이다),In the present invention, the term " hypenesine " is used to refer to a compound of the formula C 21 H 44 and salts thereof. In the present invention, the term "salt" means a salt (obtained, for example, by reaction with an acid or a base) of a physiologically tolerable compound of the present invention in which the target (eg, target plant) is physiologically tolerated. Salts of the compounds of the present invention may be derived from organic or inorganic acids and bases. Examples of acids include hydrochloric, hydrobromic, sulfuric, nitric, perchloric, fumaric, maleic, phosphoric, glycolic, lactic, salicylic, succinic, toluene- But are not limited to, formic acid, benzoic acid, malonic acid, sulfonic acid, naphthalene-2-sulfonic acid, benzenesulfonic acid and the like. Other acids, such as oxalic acid, which are not acceptable by themselves, may also be used in the preparation of salts useful as intermediates for obtaining the compounds of the present invention and their addition salts. Examples of bases include, but are not limited to, alkali metal (e.g., sodium) hydroxides, alkaline earth metal (e.g., magnesium) hydroxides, and ammonia. Examples of salts include, but are not limited to, acetate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, citrate, camphorate, camphorsulfonate, cyclopentane propionate, digluconate, dodecyl sulfate, But are not limited to, ethane sulfonate, fumarate, flucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, chloride, bromide, iodide, 2-hydroxyethanesulfonate, lactate, Sulfonate, 2-naphthalenesulfonate, nicotinate, oxalenite, pamoate, pectinate, persulfate, phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiosia But are not limited to, nitrate, tosylate, undecanoate, and the like. Examples of other salts include Na +, NH 4 +, and NW 4 + (where W is an alkyl group of Cl-C4)
[식물 및 식물 생장 촉진][Promotion of plant and plant growth]
본 발명에 따른 조성물은 다양한 식물에 대해 적용될 수 있으며, 적용된 식물에 대해 우수한 생장 촉진 효과를 나타낼 수 있다. 상기 식물은 단자엽 또는 쌍자엽 식물이 포함될 수 있으며 예를 들어, 담배, 오이, 고추, 토마토, 배추, 밀 또는 보리가 포함되나, 이에 제한되지 않는다. The composition according to the present invention can be applied to various plants and can exhibit excellent growth promoting effect on applied plants. The plant may include a terminal leaf or a twin leaf plant, for example, but not limited to, tobacco, cucumber, pepper, tomato, Chinese cabbage, wheat or barley.
본 발명에 있어서, 용어 '식물 생장'은 식물의 발아, 각 기관(뿌리, 줄기, 잎, 열매 등)의 분화, 개화 또는 발육, 그리고 크기 및 무게 증진과 관련된 것을 의미한다. In the present invention, the term " plant growth " refers to the differentiation, flowering or growth of plants, germination, organs (roots, stems, leaves,
또한, 본 발명에 있어서, 용어 '식물 생장 촉진'은 식물 생장의 자극, 속도 증진, 정도의 향상이 포함된 의미이다. In the present invention, the term " promoting plant growth " is meant to include stimulation of plant growth, speed enhancement, and improvement in degree.
[조성물][Composition]
본 발명에 따른 조성물은 하이네코사인을 유효성분으로 포함한다. 조성물 내 하이네코사인 농도는 5 ~ 50ng인 것이 바람직하나, 대상 식물의 종류, 육묘 환경 경제성 등을 고려하여 통상의 기술자가 적절히 선택할 수 있다. The composition according to the present invention contains heneicosane as an active ingredient. The concentration of the herbicosane in the composition is preferably 5 to 50 ng, but it can be suitably selected by a person skilled in the art in consideration of the type of plant to be treated, the economical efficiency of seedling growing, and the like.
본 발명에 따른 조성물에는 본 발명의 목적을 저해하지 않는 이상 하이네코사인 외에 다른 성분을 추가로 포함할 수 있다. 예를 들어, 식물 생육 촉진용 조성물로 적합한 부형제 또는 담체를 추가로 포함할 수 있으며, 당업계 공지된 다양한 식물 생육 촉진용 성분을 추가로 포함할 수 있다. The composition according to the present invention may further contain other components besides the hyperene cosine, without impairing the object of the present invention. For example, the composition may further include an excipient or carrier suitable as a composition for promoting plant growth, and may further include various plant growth promoting components known in the art.
본 발명에 따른 조성물은 당업계 공지된 다양한 방법으로 식물에 적용될 수 있다. 예를 들어, 식물 전체에의 처리, 식물근(root) 처리, 식물 종자 처리, 번식기 중의 식물 조직 처리, 또는 식물 파종이나 식수 전후에 식물 생장 촉진용 조성물 매체에 포함시켜 처리하는 방법이 포함될 수 있으며, 이에 제한되지 않고, 당업계 공지된 다양한 방법으로 상기 식물, 식물 주변 토양 또는 식물 주변 공기 중에 처리될 수 있다. 또한, 상기 식물, 식물 주변 토양 또는 식물 주변 공기 중에의 처리는 살포(예를 들면 분무, 미스팅, 아토마이징, 분말 살포, 과립 살포, 수면시용, 상시용 등), 토양시용(예를 들면 혼입, 관주 등), 표면시용(예를 들면 도포, 도말법, 피복 등), 침지, 독이, 훈연 시용 등에 의해 행할 수 있다. 당업계 공지된 다양한 방법으로 수행될 수 있으며, 적용 방법 및 사용량은 육묘 환경, 계절, 대상 식물의 종류, 또는 경제성 등을 고려하여 통상의 기술자가 적절히 선택할 수 있다. The composition according to the present invention can be applied to plants by various methods known in the art. For example, the method may include a treatment for the entire plant, a root treatment, a plant seed treatment, a plant tissue treatment in a breeding period, or a treatment for a plant growth promoting composition medium before or after planting or drinking water , But not limited thereto, can be treated in various ways known in the art in the plant, in the soil surrounding the plant, or in the air around the plant. In addition, the treatment of the plant, the soil around the plant or the air around the plant may be performed by spraying (for example, spraying, misting, atomizing, powder spraying, granule spraying, (E.g., coating, smearing, coating or the like), immersion, poisoning, smoke application, or the like. May be carried out by various methods known in the art, and the application method and amount thereof may be suitably selected by a person skilled in the art in consideration of the seedling setting environment, the season, the kind of the target plant, or the economy.
본 발명에 따른 조성물은 다양한 식물 생육 촉진 제제로 활용될 수 있다. 상기 제제에는 예를 들어, 농약, 비료, 종자코팅제 또는 토양개량제 등이 포함될 수 있다. 본 발명에 따른 제제는 여러가지 형태로 제조될 수 있으며, 예컨대 안정적인 제제화를 목적으로 수화제, 입제, 캡슐제 또는 유제의 형태로 제조될 수 있다. The composition according to the present invention can be utilized as various plant growth promoting agents. Such formulations may include, for example, pesticides, fertilizers, seed coatings or soil conditioners. The preparation according to the present invention may be prepared in various forms, for example, in the form of a wettable powder, a granule, a capsule or an emulsion for the purpose of stable formulation.
본 발명에 따른 하이네코사인을 유효성분으로 포함하는 조성물을 식물에 적용하면, 식물의 생장을 촉진시킬 수 있으며, 따라서 다양한 식물 생장 촉진용 제제로 활용될 수 있다. When the composition comprising the herbicidal composition according to the present invention as an active ingredient is applied to a plant, it can promote the growth of the plant, and thus can be utilized as a formulation for promoting various plant growth.
본 명세서에 첨부되는 다음의 도면들은 본 발명의 바람직한 실시예를 예시하는 것이며, 전술한 발명의 내용과 함께 본 발명의 기술사상을 더욱 이해시키는 역할을 하는 것이므로, 본 발명은 그러한 도면에 기재된 사항에만 한정되어 해석되어서는 아니 된다.
도 1은 하이네코사인(heneicosane)의 식물 생장 촉진 효과를 확인한 것으로, 상단은 하이네코사인 적용시 식물의 변화 사진을 도시한 것이고, 하단은 5, 50 및 500 ng 의 농도로 하이네코사인에 노출된 식물의 생중량을 그래프로 나타낸 것이다. 각 그래프 위의 문자(bc, a, c)는 통계학적으로 다른 수치를 나타낸다 (P = 0.05) 수직선은 표준 오차를 나타낸다. BRIEF DESCRIPTION OF THE DRAWINGS The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate exemplary embodiments of the invention and, together with the description of the invention, It should not be construed as limited.
FIG. 1 shows the effect of accelerating the plant growth of heneicosane. The upper part shows a photograph of the change of the plant when the heneicosane was applied and the lower part shows the plant exposed to the heneicosane at the concentration of 5, 50 and 500 ng It is a graphical representation of the raw weight. The letters (bc, a, c) on each graph represent statistically different values ( P = 0.05). Vertical lines represent standard errors.
이하, 본 발명을 구체적으로 설명하기 위해 실시예 및 실험예를 들어 상세하게 설명하기로 한다. 그러나 본 발명에 따른 실시예 및 실험예는 여러 가지 다른 형태로 변형될 수 있으며, 본 발명의 범위가 아래에서 상술하는 실시예 및 실험예에 한정되는 것으로 해석 되어서는 안 된다. 본 발명의 실시예 및 실험예는 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해서 제공되는 것이다.Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the embodiments and experimental examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments and experiments. The embodiments and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.
실시예 1. 하이네코사인의 식물 생장 촉진 효과 시험Example 1. Plant growth promotion effect test of hypenecosene
하이네코사인(heneicosane) (Sigma Aldrich, CA, USA) 을 다른 농도에서 아이-플레이트 분석(I-plate assay)을 실시하였다. Heneicosane (Sigma Aldrich, CA, USA) was subjected to I-plate assay at different concentrations.
하이네코사인을 3개의 농도(5, 50 및 500ng)로 메탄올에 용해하고, 이를 아이-플레이트의 절반 위 멸균된 종이 디스크 내에 넣었다. 대조군으로는 메탄올을 이용하였다. 플레이트의 다른 쪽 절반에는 표면 멸균된 담배 종자를 두었다. 플레이트를 밀봉하고 12 h 광/12 h 암 조건하에서 25℃에서 배양하고, 4주 후에 식물의 생중량을 측정하였다. The heneicosane was dissolved in methanol at three concentrations (5, 50 and 500 ng) and placed in a disc of sterilized paper that was half the size of the eye-plate. Methanol was used as a control. On the other half of the plate, surface-sterilized tobacco seeds were placed. The plate was sealed and incubated at 25 ° C under 12 h light / 12 h dark conditions, and the bio weight of the plants was determined after 4 weeks.
그 결과, 3개 농도 모두에서 하이네코사인은 대조군에 비해 담배 식물의 성장을 촉진시킴을 확인하였다. 하이네코사인(50ng)에서 담배 식물의 생중량은 3배 증가하였다. As a result, it was confirmed that henececone accelerated growth of tobacco plants in all three concentrations compared with the control group. The live weight of tobacco plants in heinecosene (50 ng) increased three-fold.
실시예 2. 통계 분석Example 2. Statistical analysis
데이터는 SAS JMP software (SAS Institute, Cary, NC, USA)를 사용해 변이 분석하였다. 각 샘플의 처리 수단에 따른 상당한 차이는 P = 0.05 에서 Least Significance Difference (LSD) Test 를 사용해 결정하였다. 모든 실험은 2회 이상 실시하였다. 각 실험에서의 데이터는 개별 분석하였다. Data were analyzed using SAS JMP software (SAS Institute, Cary, NC, USA). Significant differences according to the processing means of each sample were determined using the Least Significance Difference (LSD) Test at P = 0.05. All experiments were performed at least twice. Data from each experiment were analyzed individually.
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