KR101837960B1

KR101837960B1 - Anti-wrinkle composition comprising extracts of nelumbo nucifera roots, and willow or propolis

Info

Publication number: KR101837960B1
Application number: KR1020150132382A
Authority: KR
Inventors: 이지원; 박진오; 이지연; 김덕하; 윤철수
Original assignee: 유씨엘 주식회사
Priority date: 2015-09-18
Filing date: 2015-09-18
Publication date: 2018-03-13
Also published as: KR20170034112A

Abstract

The present invention relates to a wrinkle-improving composition containing a lotus root extract and a willow extract or propolis, wherein the lotus root extract; And at least one selected from willow extract and propolis extract as an active ingredient. The synergistic action of the effective ingredient maximizes the effect of pro-collagen synthesis and / or elastase inhibition, and ultimately, Can be improved.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a wrinkle-improving composition containing a lotus root extract and a willow extract or a propolis.

The present invention relates to a wrinkle-removing composition containing a lotus root extract and a willow extract or propolis.

The human skin undergoes changes due to various internal and external factors as it gets older. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and ozone layer destruction Increase of ultraviolet ray reaching the surface and environmental pollution are further intensified, and free radicals and active noxious oxygen are generated so that wrinkles are increased, elasticity is decreased, and the number of spots, freckles and black blotches is increased. .

However, because modern society attaches importance to youth and beauty, it wants to delay the aging of the skin caused by internal or external factors, or to restore the aging that has already progressed, in order to improve the quality of life. Therefore, various wrinkle improving agents, elasticity improving agents, whitening agents, and the like are being developed.

As the aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid, proteoglycans, glycosaminoglycan, fibronectin and glycoprotein, which are constituents of the skin, are changed or decreased and the ability to bind with water is decreased, , Skin dryness, and skin sagging. In addition, collagen is degraded due to an increase in matrix metalloproteinase (MMP), which is an enzyme that degrades skin tissue by ultraviolet rays, and biosynthesis of elastase is increased. Thus, elastin and adhesion protein It is known to decompose fibronectin to promote aging. A large amount of research has been conducted on active ingredients capable of preventing and improving such aging phenomena. Representatively, a variety of wrinkle-improving ingredients have been developed that either increase collagen or elastin, the structural proteins of the dermal layer, or prevent collagen degradation through matrix metalloprotease inhibition.

US Patent No. 5,925,348 discloses an anti-aging cosmetic composition using a seed extract of lotus flower, and Japanese Patent No. 4, 693963 contains information on promoting collagen synthesis using seed embryos of the lotus.

However, a composite composition containing a combination of willow extract or propolis with a mushroom extract is not known, and the collagen synthesis promoting effect has not yet reached a satisfactory level.

US 5925348 A (Jul 20, 1999) JP 4693963 B2 (June 1, 2011)

The inventors of the present invention have confirmed that a combination preparation obtained by mixing willow extract and / or propolis with a soft extract has a remarkable effect of accelerating collagen synthesis, compared with the respective preparations, and completed the present invention and provided a composition therefor do.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art,

Lotus root extract; And

A wrinkle-improving composition comprising at least one member selected from willow extract and propolis extract as an active ingredient.

The present invention also provides a wrinkle-improving composition characterized in that the active ingredient has a collagen synthesis promoting action and / or an elastase inhibiting action.

Further, in the present invention, the above-mentioned effective ingredient has a collagen synthesis promoting action,

The total content of the active ingredient extract is 0.5% by weight or less,

Wherein the wrinkle-reducing composition is comprised of Lotus root extract: Propolis extract: Willow extract = 0.8-1.2: 1.6-2.4: 0.8-1.2 weight ratio.

In the present invention, it is preferable that the effective ingredient has an elastase inhibiting action,

Wherein the wrinkle-reducing composition is comprised of Lotus root extract: Propolis extract: Willow extract = 1.6 to 2.4: 0.8 to 1.2: 0.8 to 1.2 weight ratio.

The present invention also provides a wrinkle-improving composition, wherein the active ingredient is contained in an amount of 5% by weight or less based on the total weight of the composition.

In addition, the composition of the present invention can be used as a cosmetic composition for cosmetics including toner, flexible lotion, convergent lotion, nutritional lotion, ampoule, lotion, essence, nutritional cream, eye cream, massage cream, cleansing cream, cleansing lotion, foaming wash, cleansing foam, , Cleansing gel, wash off pack, peel off pack, sheet mask pack, hydrogel mask pack, lipo gel mask pack, clay mask pack, soap, spray, line cream, sun lotion, Make-up base, foundation, and primer product.

INDUSTRIAL APPLICABILITY The present invention has the effect of maximizing the effect of procollagen synthesis and / or the inhibition of elastase by synergistic action between the active ingredients, and ultimately improving skin wrinkles.

Fig. 1 is a graph showing the results of the production of procollagen by concentration.
2 is a graph showing cytotoxicity results by concentration.
3 is a graph showing cytotoxicity results at a concentration of 2%.
4 is a graph showing the results of inhibition of elastase at a concentration of 2%.

Hereinafter, the present invention will be described in detail.

According to the present invention,

Lotus root extract; And

Wrinkle extract and propolis extract as an active ingredient.

The above-mentioned effective ingredient has a collagen synthesis promoting action and / or an elastase inhibiting action, and can give a wrinkle improving effect.

From the viewpoint of promoting collagen synthesis, it is preferable that the total content of the active ingredient extract is lower than 0.5% by weight (when the concentration is high, cytotoxicity is manifested and the collagen synthesis effect is also inferior). Lotus root extract: Propolis extract: = 0.8 to 1.2: 1.6 to 2.4: 0.8 to 1.2 weight ratio is preferable because it is the combination having the strongest synergistic effect. A more detailed understanding of this may be made through the following Examples and Experimental Examples.

From the viewpoint of inhibition of elastase, it is preferable that the composition contains Lotus root extract: Propolis extract: Willow tree extract = 1.6-2.4: 0.8-1.2: 0.8-1.2 by weight because it is the combination having the strongest synergy. A more detailed understanding of this may be made through the following Examples and Experimental Examples.

The active ingredient is preferably contained in an amount of 5% by weight or less based on the total weight of the composition, because cytotoxicity is expressed at a high concentration. Especially, the toxicity is remarkable as the concentration of lotus root extract is increased. A more detailed understanding of this may be made through the following Examples and Experimental Examples.

The compositions of the present invention can be made into a wide variety of cosmetic formulations and related products by incorporating into formulations or products of the compositions. Non-limiting examples thereof include toners, softening lotion, convergent lotion, nutritional lotion, ampoule, lotion, essence, nutritional cream, eye cream, massage cream, cleansing cream, cleansing lotion, foaming wash, cleansing foam, cleansing water, cleansing gel Liposome mask pack, clay mask pack, soap, spray, sun cream, sun lotion, sunbath, sun mist, bevy cream, makeup base, wash-off pack, peel-off pack, sheet-like mask pack, hydrogel mask pack, , Foundations, and primer products.

Hereinafter, the present invention will be described in more detail with reference to examples. The following examples are for the purpose of illustrating the present invention and are not intended to limit the scope of the present invention.

Example

Production of Lotus root extract

The dried root was washed with purified water, dried and sieved. Purified water and ethanol 3: 7 mixed solvent were extracted by adding about 10 times volume. A cooling condenser was installed to prevent the solvent from evaporating and was extracted by heating for 12 hours while maintaining a temperature of 60 to 80 캜. The extract was filtered with 400 mesh filter cloth, and the residue was extracted once more by the same method. The extract was cooled to room temperature and filtered through a filter paper of Wattman No. 2. The filtrate was filtered through a filter paper of Wattman No. 2 and filtered using a distillation apparatus (Buchi Rotavapor R-124, Water Bath B-480 Made in Switzerland, Eyela A-3S Tokyo rikakikai) The mixture was distilled while maintaining the temperature at 80 DEG C, and the solvent was evaporated. The solvent was concentrated under reduced pressure, and then lyophilized to obtain an extract powder of the dried root.

Production of willow extract

The freeze-dried powder was obtained through the same procedure as in Example 1 except that the willow skin was used.

Preparation of propolis extract

Propolis powder was washed thoroughly with water, and the propolis powder was prepared by passing propolis powder through the filter after extracting the propolis by stirring the cornstarch and the cut propolis raw material in a stirrer.

The extracted extracts were mixed at the mixing ratios shown in Tables 1 and 2 to prepare extract bases having various mixing ratios.

Mixing weight ratio Sample name H1 H2 H3 H4 H5 H6 H7 Lotus root
extract One - - One One One 2 Propolis extract - One - One One 2 One Willow extract - - One One 2 One One

Mixing weight ratio Sample name H8 H9 H10 H11 H12 H13 Lotus root
extract One One 2 One One 2 Propolis extract One 2 One - - - Willow extract - - - One 2 One

The prepared extract base was mixed with the prescribed excipients as shown in Tables 3 and 4 to finally prepare a cosmetic composition.

Example 1

Raw material name Content (wt%) water Balance glycerin 10 1,3-butylene glycol 10 EDTA-2Na 0.05 Oil 20 H6 extract base 0.1

Example 2

Raw material name Content (wt%) water Balance glycerin 10 1,3-butylene glycol 10 EDTA-2Na 0.05 Oil 20 H7 extract base 5

Experimental Example

The prepared extract base was subjected to evaluation of the effect of pro-collagen formation and cytotoxicity, elastase inhibitory effect and cytotoxicity evaluation by the following concentrations.

Concentration of procollagen production by concentration

Collagen is synthesized in the cell as procollagen, secreted extracellularly and polymerized into collagen fibers. At this time, since the N-terminal and C-terminal propeptides of procollagen are released by the endopeptidase, human procollagen type I C-terminal peptide (PIP) was quantitated to confirm the amount of collagen production.

- Way:

1) A fibroblast cell (CCD-986SK) was seeded in a 48-well plate at 5 × 10 ⁴ cells / well.

② After incubation for 24 hours, the cells were washed with PBS.

③ Sample was treated with serum-free medium.

④ cultured for 24 hours.

⑤ The culture supernatant was obtained and the amount of procollagen type I C-peptide was measured. Procollagen Type I C-peptide (PIP) Proceed according to the EIA kit method (TaKaRa MK101).

The results are shown in Table 5 and Fig.

collagen production (%) SD (%) control 100.00 2.218 0.1% H1 108.92 1.018 H2 109.65 1.012 H3 102.51 3.246 H4 103.59 1.452 H5 113.66 0.976 H6 120.60 1.839 H7 109.21 1.016 1.0% H1 104.29 0.303 H2 119.64 0.746 H3 121.08 2.794 H4 109.41 8.150 H5 108.96 8.301 H6 109.42 2.397 H7 92.82 2.169 5.0% H1 50.23 0.640 H2 120.40 3.777 H3 134.52 2.074 H4 11.63 14.209 H5 -0.89 -46.725 H6 6.78 9.570 H7 -1.56 -36.691

As the concentration of Willow extract and Propolis extract increased, the amount of collagen synthesis increased. Especially, when the extracts were treated with 0.1% of each extract, the highest promoting effect of collagen synthesis was shown at 1: 2: 1 ratio of lotus root extract: propolis extract: willow extract.

Evaluation of cytotoxicity by concentration MTT assay

Living cells convert MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-dipheny-Ltertrazolium bromide) into a bule formazon product by the action of the dehydrogenase in mitochondria, The product was eluted with a solvent, and the relative growth of living cells was measured by measuring the absorbance at 540 nm using ELISA. The specific confirmation method was as follows.

- checking way:

1) RAW264.7 cells were adjusted to 1.0 × 10 ⁵ cells / ml using DMEM medium, and then inoculated into 48-well plates and cultured for 18 hours.

(2) A new medium containing the test substance and LPS (1 μg / ml) was treated at the same time and cultured for 24 hours.

③ 100 ㎕ of MTT solution (2 ㎎ / ㎖) was added and reacted at 37 ℃ for 2-4 hours.

④ Remove the medium and dissolve 300 ~ 500 μl of DMSO crystals.

⑤ Absorbance was measured at 540 nm.

The results were as shown in Tables 6 to 7 and Figs. 2 to 3 below.

Cell viability (%) SD (%) control 100.00 3.227 0.1% H1 103.27 3.738 H2 102.69 3.508 H3 104.55 1.628 H4 104.49 1.055 H5 106.16 2.140 H6 105.74 0.591 H7 106.85 0.607 1.0% H1 107.81 0.702 H2 108.03 0.528 H3 109.70 0.943 H4 110.01 0.915 H5 103.27 3.738 H6 102.69 3.508 H7 104.55 1.628 5.0% H1 53.61 1.711 H2 108.03 0.528 H3 109.70 0.943 H4 13.26 1.452 H5 15.28 1.314 H6 18.86 0.576 H7 15.09 0.070

Cell viability (%) SD (%) control control 100.00 0.315 2.0% H1 102.73 3.722 H2 106.33 1.593 H3 106.63 0.355 H4 105.24 3.324 H5 107.71 0.641 H6 106.11 3.016 H7 105.48 0.893 H8 106.81 0.202 H9 110.85 1.674 H10 107.93 1.186 H11 110.54 0.955 H12 104.54 4.181 H13 103.41 1.650 0.50% Vitamine C 105.18 1.542

In the case of lotus root extract, the collagen synthesis promoting effect was shown, but it was toxic as the concentration increased. However, it is considered that the concentration of the lotus root extract is preferably 5% or less, more preferably 4% or less, since it does not show toxicity at about 2.0%.

Elastase Inhibitory effect experiment

Elastase, known as an elastin degrading enzyme, is known as an enzyme that catalyzes the cleavage of fused bonds by breaking down elastin, an elastic fiber protein, to break the network of the dermal tissue of the skin. Therefore, it is considered that skin aging can be suppressed by lowering the elastase activity, and the effect of inhibiting the elastase activity can be confirmed and the skin wrinkle improving effect of the extract can be confirmed. The amount of p-nitroanilide produced by using N-succinyl-Ala-Ala-p-nitroanilide (Sigma) as a substrate was measured.

- Way:

① Add 100 μl of each extract to a 96-well plate at a concentration of 2.0%.

② Add 60 μl of 0.2 M Tris buffer (pH 8.0).

③ 20 μl of 5 mM N-Succinyl-Ala-Ala-p-nitroanilide as a substrate was added.

④ 20 μl of enzyme, 10 μg / ml elastase, was added.

(5) The reaction was carried out at 25 ° C for 15 minutes.

⑥ The amount of p-nitroaniline produced at 410 nm was measured.

* Positive control: Vitamine C 0.5%

The results were as shown in Table 8 and Fig.

Inhibition (%) SD (%) control 0.00 2.079 2.0% H1 23.63 0.266 H2 3.43 2.383 H3 3.25 0.120 H4 25.86 0.443 H5 31.62 0.990 H6 29.64 1.635 H7 44.03 2.505 H8 23.99 1.934 H9 23.66 1.203 H10 34.39 0.516 H11 19.44 1.872 H12 25.21 0.323 H13 35.70 0.436 0.50% Vitamine C 54.37 2.098

In the evaluation of inhibition of elastase activity, lotus root extract showed the highest inhibitory effect on elastase activity. In particular, promoter of collagen synthesis was promoted at the ratio of 2: 1: 1 of lotus root extract: propolis extract: willow tree extract .

Claims

Lotus root extract; And
Which contains at least one selected from willow extract and propolis extract as an active ingredient Wherein the effective ingredient is at least one selected from the group consisting of
(a) Lotus root extract: Propolis extract = 1.6 ~ 2.4: 0.8 ~ 1.2 weight ratio;
(b) Lotus root extract: Willow extract = 1.6 ~ 2.4: 0.8 ~ 1.2 weight ratio; or
(c) Lotus root extract: Propolis extract: Willow extract = 1.6 to 2.4: 0.8 to 1.2: 0.8 to 1.2 weight ratio,
Wherein the active ingredient has an elastase inhibitory action,
Wherein the active ingredient is present in an amount of more than 0% by weight and not more than 5% by weight based on the total weight of the composition.

delete

A composition comprising the composition of claim 1,
Cleansing Lotion, Foaming Wash, Cleansing Foam, Cleansing Water, Cleansing Gel, Wash-off Pack, Peel-off, Lotion, Lotion, Essence, Nourishing Cream, Eye Cream, Massage Cream Such as a pack, a sheet-like mask pack, a hydrogel mask pack, a liposcel mask pack, a clay mask pack, a soap, a spray, a line cream, a sun lotion, a sunbath, a sun mist, a babe cream, a makeup base, &Lt; RTI ID = 0.0 > cosmetic < / RTI > composition.