KR101818837B1 - Pest control composition comprising allyl mercaptan against stored product insect - Google Patents
Pest control composition comprising allyl mercaptan against stored product insect Download PDFInfo
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- KR101818837B1 KR101818837B1 KR1020160019166A KR20160019166A KR101818837B1 KR 101818837 B1 KR101818837 B1 KR 101818837B1 KR 1020160019166 A KR1020160019166 A KR 1020160019166A KR 20160019166 A KR20160019166 A KR 20160019166A KR 101818837 B1 KR101818837 B1 KR 101818837B1
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- allyl mercaptan
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- pest
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
Abstract
The present invention relates to a low-pesticidal composition for controlling insect pests comprising Allyl mercaptan as an active ingredient and a method of controlling the same. The controlling composition according to the present invention can solve problems such as environmental destruction or accumulation in the body caused by the treatment of existing chemical compounds by using allyl mercaptan derived from a natural material, garlic essential oil, as an active ingredient.
Description
The present invention relates to a low pesticide composition for controlling insect pests comprising allyl mercaptan, and a low pest control method for treating the composition.
Pest insects have been a major hurdle to the food industry by promoting the quality and quantitative loss of food in various food groups including cereals as well as processed foods. According to a study conducted by the Korea Food & Drug Administration, 46.4% of insects and pests were found to have the highest frequency of reported foreign substances in food.
Among the pest insects, rice weevils and hananggom moths are distributed not only in Korea but also in the whole world, and they are the key pests causing the greatest damage to stored grains.
Unusually, in the case of haploid moths, unlike general pests, the growth stage, which is particularly problematic for food safety, is the larvae stage. The moth larvae have sharp and powerful jaws, It is because.
In general, chemical compounds such as methyl bromide and phosphine are mainly used for insect pest insects, but these chemical compounds have problems such as environmental destruction and accumulation in the body. Accordingly, a method for controlling low-pest insects using essential oils derived from natural materials rather than chemical compounds has been developed, and Korean Patent Laid-Open No. 2002-0087568 discloses insecticidal compositions using plant extracts.
The inventors of the present invention confirmed that allyl mercaptan has a superior insecticidal effect against insect pests while investigating insecticides of insect pests.
Accordingly, an object of the present invention is to provide a composition for controlling low pest insects, which comprises allyl mercaptan as an active ingredient.
However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.
In order to achieve the above object, the present invention provides a composition for controlling low pest insects, which comprises allyl mercaptan as an active ingredient.
In one embodiment of the present invention, the insect pest is a rice weevil ( Sitophilus oryzae or Plodia interpunctella .
In another embodiment of the present invention, the allyl mercaptan is contained in an amount of 0.1 to 20% (v / v) based on the total composition.
In another embodiment of the present invention, the composition further comprises an organic solvent.
In another embodiment of the present invention, the organic solvent is an organic solvent selected from the group consisting of acetone, ether, isooctane, and hexane.
The present invention also provides a low-pest control method for treating the low-pest control composition.
In one embodiment of the present invention, the treatment of the composition is characterized by being carried out by fumigation.
The present invention is a low-pesticidal composition for controlling insect pests comprising allyl mercaptan as an active ingredient, and can be utilized as a natural insect repellent as an environmentally friendly essential ingredient of essential oils derived from natural products. It was confirmed that the controlling composition of the present invention not only has excellent insecticidal effect against insect pests, but also has a good avoidance effect. Therefore, when the composition of the present invention is used, it is expected that the composition can be safely controlled without accumulation in the body or environmental destruction, which is a problem when conventional chemical compounds are used.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram of an experiment for measuring fumigantive insecticidal activity of allyl mercaptan on rice weevils. FIG.
FIG. 2 is a graph showing the results of confirming the fumigation killing effect of garlic essential oil and allyl mercaptan on rice weevil.
Fig. 3 is a schematic diagram of an experiment for measuring the fumigantive insecticidal activity of allyl mercaptan on the moth larvae.
FIG. 4 is a graph showing the results of fumigation killing effect of allyl mercaptan on the larvae moth larvae.
FIG. 5 is a graph showing the results of an experiment for inhibiting acetylcholinesterase activity of garlic essential oil and allyl mercaptan on rice weevil.
FIG. 6 is a graph showing the results of an experiment for inhibiting the acetylcholinesterase activity of garlic essential oil and allyl mercaptan on the moth larvae.
FIG. 7 is a schematic view of an experiment for measuring the erectile force of the insect pest against garlic essential oil and allyl mercaptan.
Fig. 8 is a graph showing the results of an experiment to measure the repellency of rice weevil against allyl mercaptan.
Fig. 9 is a diagram showing the results of an experiment to measure the repellency of rice weevil against garlic essential oil.
FIG. 10 is a view showing the results of the experiment for measuring the rice weevil period of the control (control).
Fig. 11 is a graph showing the results of an experiment for measuring the erectile force of the larvae moth larvae against allyl mercaptan and garlic essential oil.
In the present invention, as a demand for a insect-resistant material derived from a natural substance for a low-pest insect has been increased, while studying a natural substance having an excellent low-pest insect control effect, among the monomolecular substances of garlic essential oil, The present invention has been completed.
That is, it is an object of the present invention to provide a composition for controlling low pest insects, which contains allyl mercaptan as an active ingredient.
The 2-propene-1-thiol (Allyl mercaptan) of the present invention is represented by the following general formula (1).
≪ Formula 1 >
The allyl mercaptan may be one separated from an essential oil derived from a natural product, and the allyl mercaptan may be suitably separated according to a conventional method, or a chemically synthesized allyl mercaptan may have the same effect .
In the present invention, the insect pests to be controlled include rice weevil ( Sitophilus oryzae and Plodia interpunctella ), but are not limited to the boredom. Especially rice weevils have high insecticidal power against adults. Insecticidal activity is confirmed in the third instar larvae of moths, which can be used as a more effective composition for controlling, considering that the moths of larvae are particularly problematic for food safety.
The allyl mercaptan is used in a state dissolved in an organic solvent and may be contained in an amount of 0.1 to 20% (v / v) based on the total composition. (V / v), preferably 0.1 to 10% (v / v), more preferably 0.3 to 5% (v / v) for rice weevils and 0.1 to 15% But is not limited to these concentrations.
The organic solvent may be acetone, ether, isooctane, hexane, or the like, but is not limited to the above-mentioned kinds.
The composition for controlling insect pests of the present invention may be added with commonly used additives.
Further, the present invention can provide a method for controlling low-pest insect pests, which is capable of treating the low-pest control composition.
The treatment of the composition may be carried out by fumigation, or simply by applying the composition to the surface. For example, a method of coating the composition on the surface of the packaging material of the grain may be used.
Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the examples.
[ Example ]
Preparation for experiment
(1) garlic oil
The garlic essential oil used in this example was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, Mo., USA), and the oil used for comparison was purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA).
(2) Allyl mercaptan
The allyl mercaptan used in this example is commercially available from Sigma-Aldrich Co., Ltd. 2-propene-1-thiol (allyl mercaptan) purchased from St. Louis, MO, USA was used. Other compounds used for comparison are also available from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA).
Example 1 Measurement of Fumigation Potential of Allyl Mercaptan on Rice Weevil
The fumigant insecticidal test carried out in the present embodiment means an experimental method for confirming whether a gaseous insecticide acts on a target insect to exhibit insecticidal ability. A schematic diagram of the experiment is shown in Fig.
First, garlic essential oil and allyl mercaptan were diluted with 0.4% (v / v) of acetone, respectively, and fumigantive insecticidal power against rice weevils was measured. Rice weevils were cultured at laboratory level under the condition that they were fed with brown rice and were not exposed to any insecticides.
To determine the fungicidal activity of garlic essential oil and allyl mercaptan, 10 (0.4% (v / v)) pesticides were placed in a glass bottle with a diameter of 3 cm and a height of 11 cm, (50 mg) of filter paper (whatman NO. 40) with a diameter of 2 cm and dried for 3 minutes, and then attached to the lid of the glass vial at 24-hour intervals for 7 days %) Were observed. The reason for putting the food together is to prevent the risk of asexion of rice weevil, and the variable asana is a significant negative factor that must be removed in order to obtain accurate experimental results. The fumigant insecticide experiments were repeated three times each.
Referring to FIG. 2, it was confirmed that the insecticidal effect of the insecticide material increases as the storage period increases. Allyl mercaptan showed better insecticidal effect than garlic essential oil. This value was the best when compared with the insecticidal effect of other plant essential oils and single substances, thus proving the applicability of allyl mercaptan to insecticide.
Example 2: Moth Fumigation of allyl mercaptan on larvae Insecticide Measure
Garlic essential oil and allyl mercaptan were diluted with acetone to 10% (v / v), respectively. A schematic diagram of the experiment is shown in Fig. 20 glass bottles with a diameter of 1.5 cm and a height of 4.5 cm were added with a third larva of a moth larva and three eggs of brown rice for preventing asana. Each glass bottle was covered with calico and then closed with a lid of 1.1 cm diameter drilled to induce air circulation. The filter paper with a diameter of 70 mm was dispensed with 300 μl of diluted insecticide and attached inside a 0.5 L polyethylene container lid. After loading 20 glass bottles with the moth larvae in a 0.5 L polyethylene container, the lid with filter paper containing the insect repellent material was closed and placed in the dark room at 28 ° C to measure the mortality rate (24 hours and 120 hours) %) Were observed. The fumigant insecticide experiments were repeated three times each.
As can be seen from FIG. 4, it was confirmed that the insecticidal effect was increased as the storage period of all the materials increased. The mortality rate of allylmercaptans was higher than that of the control, indicating that allylmercaptan had an excellent insecticidal effect.
Example 3: Rice with weevil Moth Inhibition of acetylcholine hydrolase activity of allyl mercaptan on larvae
Thirty (30) of the target pests (rice weevil or hapan moth larva) were homogenized in 1 ml of phosphate buffer, centrifuged and the supernatant was used as an enzyme. The enzymes are reacted with allyl mercaptan to inactivate the enzyme and utilize the property that 5,5'-dithio-bis (2-nitrobenzoic) acid (DTNB) reacts with the substrate by using acetylcholine iodide (ATChI) And the degree of inhibition of the enzyme was measured. Absorbance was checked at 405 nm for 20 minutes at 30 sec intervals to measure maximum velocity (V). Experiments were repeated three times each.
- Inhibition of acetylcholinesterase activity (%):
The neurotransmitter, acetylcholine, is a chemical substance that transmits nerve stimulation at the nerve end and plays an important role in the growth of insects. Essential oils with insecticidal properties are known to inhibit the activity of acetylcholine hydrolase, an enzyme that degrades acetylcholine, to block the flow of neurotransmission, leading to death. The inhibitory activity of acetylcholinesterase activity of garlic essential oil and allyl mercaptan on the target insect pests was measured.
As shown in Fig. 5, the allyl mercaptan showed a superior value to the acetylcholine hydrolase of rice weevil than the garlic essential oil, which was about 85%.
As can be seen from Fig. 6, allyl mercaptan was superior to garlic essential oil in inhibiting the acetylcholinesterase activity of the moth larvae, and this value was about 55%.
The results of the above experiments are consistent with the results of the fumigant insecticidal activity tests of Examples 1 and 2, suggesting that fumigation death was due to the inhibition of acetylcholinesterase activity of the target pests.
Example 4: for allyl mercaptan Rice with weevil Moth Measuring the repulsive force of larvae
The fourth embodiment is carried out by using the mechanism for measuring the initial force shown in Fig. Evaporation experiments are experiments to determine the extent to which target insects avoid emissions within a measuring cylinder. In the experiment conducted with rice weevil, the garlic essential oil and allyl mercaptan diluted to 5% (v / v) and 2% (v / v) concentration were used with acetone. (V / v) diluted garlic essential oil and allyl mercaptan were used. The measuring cylinder was 37 cm in length and 5 cm in diameter. One side of the cylinder was closed and the other side was opened. From the clogged area, it is designated as A, B, and C zones, and brown rice and insect repellent material are placed in area A, and pests stored in area B (20 rice weevils, 30 wild moth larvae) In section C, the ventilation was used to induce the concentration difference of allyl mercaptan and the ventilation was blocked with calico to prevent escape of insects. As the number of larvae moved to area C with ventilation holes increased, it was interpreted as stronger, and the number of pests in each area was counted according to time (24 hours, 48 hours). Repeated experiments were repeated three times each.
Referring to FIG. 8, almost all the rice weevil individuals migrated to the C zone after 24 hours at the concentration of 5% allyl mercaptan, and the evacuation rate increased rather than 24 hours to 48 hours. In addition, allyl mercaptan exhibited excellent retention even at a low concentration of 2%. After 24 hours, most of the individuals migrated to the C zone and remained the same after 48 hours. This was much better than that of the rice weevil using the garlic essential oil of FIG. 9. FIG. 10 shows the results of the test for the control of the rice weevil in the control group, showing that the number of pests in Zone A and Zone C was similar.
Referring to FIG. 11, after 24 hours at the concentration of 2% allyl mercaptan, the larvae of the larvae migrated to the C zone, and showed excellent repellency even after 48 hours. This result was much better than the measurement of epigastric value of the white moth larvae using garlic essential oil.
The results of the above experiment show that allyl mercaptan not only has a very good depression resistance against rice weevils and mung bean moths, but also is far superior to garlic essential oil.
It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.
Claims (7)
Wherein the allyl mercaptan is contained in an amount of 0.1 to 20% (v / v) based on the total composition.
Wherein the composition further comprises an organic solvent.
Wherein the organic solvent is one organic solvent selected from the group consisting of acetone, ether, isooctane, and hexane.
Wherein the treatment of the composition is carried out by fumigation.
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