KR101800802B1 - Method for manufacturing Glycyrrhiza uralensis extract and composition for treating or preventing oral diseases comprising the extract - Google Patents

Abstract

The present invention relates to a method for producing licorice extract and a composition for preventing or treating oral diseases comprising the extract obtained therefrom. According to the present invention, it is possible to easily and easily remove glycyrrhizin from licorice and concentrate and extract substances having an effect on oral diseases. In addition, the licorice extract obtained by this method does not contain a harmful solvent and is safe for human body, and has a high inhibitory activity of S. mutans. Thus, it can be effectively applied for prevention and treatment of oral diseases including dental caries.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for preparing a licorice extract and a method for preparing the same,

The present invention relates to a method for producing licorice extract and a composition for preventing or treating oral diseases comprising the extract obtained therefrom.

More particularly, the present invention relates to a method for producing a licorice extract, which is capable of removing glycyrrhizin by using human-safe cooking oil and concentrating functional substances effective for oral diseases, and a method for preventing oral diseases Or < / RTI >

Licorice is a medicinal herb familiar to us so that there is a proverb called "licorice of drugstore". Glycyrrhiza uralensis licorice, which has a scientific name, Dongbibo, the property is described as plain, flavorless and poisonous. The sweetness of this licorice is due to glycyrrhizin, which is pharmacologically known as glycyrrhizin because of its antiallergic action, NK cell activation, interferon-free action, inhibition of gastric secretion, anti-ulcerative action, anti-inflammatory action It is known.

Since the substances contained in the licorice extract can effectively inhibit Streptococcus mutans (S. mutans), which is known to be a major causative agent of dental caries, various attempts to prevent dental caries using licorice extract I have been.

However, Wen D. et al. Have found that excessive doses of glycyrrhizin can result in severe hypertension, hypokalemia, and glycyrrhetic acid, which can cause diseases such as mineral metabolism excess that may prevent the conversion of cortisol to cortisone in the kidney ) Were reported to require caution because they can be overproduced (Separation methods for antibacterial and antirheumatism agents in plant medicines. J Chromatogr B Analyt Technol Biomed Life Sci 2004; 812: 101-17). The US Food and Drug Administration (FDA) regulates the amount of glycyrrhizin that can be added to food.

Therefore, SJ Ahn et al. Used HPLC to remove glycyrrhizin, which has no inhibitory effect on S. mutans in licorice extract, and which may be harmful to humans when overdosed, and that glycyrrhizin-free extract effectively inhibited S. mutans (The antimicrobial effects of deglycyrrhizinated licorice root extract on Streptococcus mutans UA159 in both planktonic and biofilm cultures. Anaerobe . 2012; 18: 590-6). However, in order to use the licorice extract obtained by using the above method as an additive, it is troublesome to remove the used organic solvent below the KFDA standard and to prove it. Due to the nature of the HPLC method, This is not easy.

In order to solve the problems of the prior art as described above, an object of the present invention is to remove glycyrrhizin from licorice by using edible oil instead of a chemical solvent harmful to the human body, to extract various functional materials in licorice, , A method for producing a licorice extract and a composition for preventing or treating an oral disease, which comprises the licorice extract obtained by the method.

According to an aspect of the present invention,

Mixing the licorice and the first water-soluble solvent to obtain a first water-soluble extract;

A second step of mixing the first aqueous extract and the edible oil to obtain an oil extract;

A third step of mixing the oil extract and the second water-soluble solvent to obtain a second water-soluble extract; And

And a fourth step of removing the second water-soluble solvent from the second water-soluble extract.

Another aspect of the present invention provides a composition for preventing or treating oral diseases, which comprises the licorice extract obtained by the above method as an active ingredient.

According to the method for preparing licorice extract of the present invention, it is possible to easily and easily remove glycyrrhizin from licorice and to extract substances which are effective for oral diseases by the difference in distribution coefficient, and it is advantageous in mass production.

In addition, the licorice extract obtained by this method does not contain harmful solvents and is safe for human body, and it is expected that it can be effectively applied for prevention and treatment of oral diseases including dental caries due to high inhibitory activity of S. mutans.

According to the method for preparing licorice extract of the present invention, it is possible to easily and easily remove glycyrrhizin from licorice and to extract substances which are effective for oral diseases by the difference in distribution coefficient, and it is advantageous in mass production.
In addition, the licorice extract obtained by this method does not contain harmful solvents and is safe for human body, and it is expected that it can be effectively applied for prevention and treatment of oral diseases including dental caries due to high inhibitory activity of S. mutans.

In the present invention, the terms first, second, etc. are used to describe various components, and the terms are used only for the purpose of distinguishing one component from another.

Moreover, the terminology used herein is for the purpose of describing exemplary embodiments only and is not intended to be limiting of the present invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprising," "comprising," or "having ", and the like are intended to specify the presence of stated features, But do not preclude the presence or addition of one or more other features, integers, steps, components, or combinations thereof.

While the invention is susceptible to various modifications and alternative forms, specific embodiments thereof are shown by way of example in the drawings and will herein be described in detail. It should be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

Hereinafter, the composition for preventing or treating oral diseases including the method for producing licorice extract of the present invention and the extract obtained therefrom will be described in detail.

Licorice (Scientific name: Glycyrrhiza uralensis ) is known to contain a number of substances that can reduce mutans streptococci. These substances are known, for example, glycyrrhizol A, licoricidin, licorisoflavan A, licorisoflavan C, licorisoflavan D, and licorisoflavan E. Eating an unsweetened lollipop containing glycyrrhizol A effectively reduces the oral streptococcal strain Have been reported.

However, glycyrrhizin, which is contained in licorice, is a glycyrrhetic acid that can induce diseases such as hypertension, hypokalemia, mineral metabolism excess, and the like when there is no mutant streptococcal inhibiting effect and excessive use of glycyrrhizin It is very important that the licorice extract be applied to the human body so as to contain only the active ingredient capable of decreasing the mutans streptococci while minimizing glycyrrhizin in the extract.

Accordingly, the inventors of the present invention have found that the content of glycyrrhizin in the oil extract extracted secondarily using an oil for the water-soluble solvent extract of licorice is lowered and the content of glycyrrhizin is lowered in functional extracts showing the inhibitory activity of S. mutans such as glycyrrhizol A The present invention has been accomplished on the basis that the functional substances are concentrated in the licorice extract by removing components of the substances and removing the water-soluble solvent from the second extract obtained by extracting the oil extract again with a water-soluble solvent.

More specifically, in the substances present in the licorice extract, glycyrrhizin is removed and used in the presence of a large difference in oil-water partition coefficient, which is one of the physical and chemical properties of these substances. The present inventors came to the present invention in consideration of the fact that the substances can be concentrated. In other words, glycyrrhizin has a relatively low oil-water partition coefficient, while other functional substances that have an effect on oral diseases have a significantly higher oil-water partition coefficient than glycyrrhizin. Glycyrrhizin is virtually insoluble in oil when it is extracted with oil due to the difference in the partition coefficient. Most of the functional materials having a large oil-water partition coefficient are dissolved in the oil.

The oil-water partition coefficients of the components present in licorice are not known, but the octanol / water partition coefficient (K _ow ) has been reported. For example, the K _ow values of glycyrrhizin, glycyrrhizol A, licoricidin, licorisoflavan A, licorisoflavan C, licorisoflavan D, and licorisoflavan E are reported as 3.194, 8.514, 7.980, 8.541, 7.771, 7.771, 7.771, respectively. The larger the value of K _ow , the more soluble it is in octanol than in water. Since octanol is nonpolar with an alcohol with 10 carbon chains, it can be assumed that the nonpolar oil will show a similar trend to the octanol-water partition coefficient. Accordingly, the present invention has been completed by applying the principle of the method of separating the substance using the difference of the octanol-water partition coefficient of the licorice extract to the method of extracting the functional substance from the licorice extract by applying to the oil.

According to one aspect of the present invention, there is provided a method for producing a water soluble extract comprising: a first step of mixing licorice and a first water-soluble solvent to obtain a first water-soluble extract; A second step of mixing the first aqueous extract and the edible oil to obtain an oil extract; A third step of mixing the oil extract and the second water-soluble solvent to obtain a second water-soluble extract; And a fourth step of removing the second water-soluble solvent from the second water-soluble extract.

First, licorice is mixed with a first water-soluble solvent to obtain a first water-soluble extract (Step 1).

At this time, the licorice may be used in powder form so that the active ingredient can be more easily dissolved.

As the first water-soluble solvent, water, a lower alcohol having 4 or less carbon atoms, or a mixture thereof may be used, preferably ethanol. In addition, when water is used as the first water-soluble solvent, hot water having a temperature of 80 ° C or higher may be used so that the active ingredients of licorice are more easily dissolved.

The mixing ratio of the licorice and the first water-soluble solvent is not particularly limited, but the weight ratio of licorice: first water-soluble solvent may be about 1: 1 to about 1:20, or about 1: 5 to about 1:10 .

According to one embodiment of the present invention, the licorice and the first water-soluble solvent are mixed well and stirred intermittently at a temperature of from room temperature to 60 ° C for a certain period of time, for example from about 12 hours to about 100 hours, To about 72 hours so that the active ingredient of licorice is sufficiently dissolved in the first water-soluble solvent. Thereafter, the mixture of the licorice and the first water-soluble solvent is filtered to remove the licorice powder to obtain only the first water-soluble extract of licorice.

Next, an edible oil is mixed with the first water-soluble extract to obtain an oil extract of licorice (step 2).

The type of the edible oil is not particularly limited. For example, the edible oil can be easily recovered from the environment such as grape seed oil, canola oil, olive oil, safflower seed oil, sunflower seed oil, corn oil, soybean oil, Oil can be used. Preferably, grape seed oil can be used.

The mixing ratio of the first water-soluble extract to the edible oil is not particularly limited, but the volume ratio of the first water-soluble extract: edible oil is about 1: 1 to about 1:20, or about 1: 1 to about 1:10 can do.

In order to obtain an oil extract of licorice, the first aqueous extract and the edible oil are thoroughly mixed, and then the mixture is stirred at a temperature ranging from room temperature to 60 ° C for a predetermined time, for example, about 1 Hour to about 48 hours, or about 6 hours to about 36 hours.

An oil extract can be obtained by confirming that the oil phase is completely separated into the first water soluble solvent layer and the oil layer and removing the first water soluble solvent layer and separating only the oil layer.

At this time, when the volume ratio of the edible oil to the first water-soluble extract is lowered, an oil extract having a high concentration of a substance having a high octane-water partition coefficient (K _ow value) among the active ingredients of licorice can be obtained, When the volume ratio of the edible oil to the water-soluble extract is increased, an oil extract having a high concentration of substances having a low octanol-water partition coefficient (K _ow value) among the active ingredients of licorice can be obtained.

By varying the volume ratio of the edible oil: first aqueous extract on the basis of the K _ow value of the functional substance to be concentrated as described above, an oil extract having a relatively higher concentration of the desired functional substance can be obtained.

Using this principle, the second step of mixing the first aqueous extract with the edible oil to obtain an oil extract may be carried out more than twice with different volume ratios of the first aqueous extract and the edible oil.

That is, the first water-soluble extract and the edible oil are mixed and then separated into an oil layer and a first water-soluble solvent layer to obtain an oil extract, and then the edible oil is mixed with the separated water- The step of obtaining the oil extract can be repeated twice or more, for example, by separating into a first water-soluble solvent layer to obtain an oil extract secondarily.

The oil extracts obtained by repeating the second step may be combined with each other, or may be separately prepared as a final licorice extract through the third and fourth steps described below.

When the mixing and separation processes of the first aqueous extract and the edible oil are carried out at different volume ratios, the functionalities extracted from the respective oil extracts according to the mixing frequency and the mixing volume ratio of the first aqueous extract and the edible oil The distribution and concentration of the substances will vary. Thus, when the extract is subjected to the extraction process several times, an oil extract having different distribution of the functional substance can be obtained for each extract. This method can be used to obtain an oil extract in which the desired active ingredients are selectively concentrated.

In order to re-extract the active ingredients dissolved in the oil from the oil extract obtained in the second step, the oil extract and the second water-soluble solvent are mixed to obtain a second water-soluble extract of licorice (step 3).

The reason why the components contained in the oil extract again are extracted with the second water-soluble solvent is that the edible oil has a very high boiling point (for example, about 220 degrees in the case of olive oil) and effectively removes the oil from the oil extract, This is because it is very difficult to obtain a secondary extract of concentrated licorice.

In order to concentrate the active ingredients again in the oil extract, the functional ingredients are re-extracted using a second water-soluble solvent which is easy to remove. Functional components with high K _ow values are better soluble in oil, but when extracted several times with a second water-soluble solvent, the active components can be transferred to the water-soluble solvent layer since they are dissolved in the second water-soluble solvent layer little by little.

More specifically, in order to obtain the second water-soluble extract, the oil extract and the second water-soluble solvent are mixed and thoroughly mixed, and then the mixture is stirred at a temperature of 60 ° C to 60 ° C for a predetermined time , For example from about 1 hour to about 48 hours, or from about 6 hours to about 36 hours.

The second water-soluble solvent layer and the oil layer are completely separated, and only the second water-soluble solvent layer is separated to obtain a second water-soluble extract of licorice.

At this time, in order to transfer more active ingredients in the oil extract to the second water-soluble solvent layer, the second water-soluble solvent layer is separated and the second water-soluble solvent is mixed again with the remaining oil layer, The second water-soluble solvent layer separated from the solvent layer and the oil layer and further re-extracted from the second water-soluble solvent layer may be added to the first water-extracted second water-soluble extract. Such a process may be repeated one or more times or two or more times as required.

The second water-soluble solvent may be water, a lower alcohol having 4 or less carbon atoms, or a mixture thereof. The first water-soluble solvent may be the same or different from the first water-soluble solvent. As the second water-soluble solvent, ethanol may preferably be used. When water is used as the second water-soluble solvent, hot water having a temperature of 80 ° C or higher may be used so that the active ingredients of licorice are more easily dissolved.

The mixing ratio of the oil extract and the second water-soluble solvent is not particularly limited, and the volume ratio of the oil extract: second water-soluble solvent is about 20: 1 to about 1:20, or about 10: 1 to about 1:10 can do.

The second water-soluble extract obtained in the third step can be used to remove the second water-soluble solvent using vacuum or the like to obtain the licorice extract of the present invention (step 4).

The thus-obtained licorice extract finally obtained through the above process is substantially free of glycyrrhizin or remains in an extremely small amount, so that the toxicity to the human body is so low that it is relatively safe even when ingested in large quantities, have. Further, it is possible to safely apply to human body by extracting only a water-soluble solvent such as ethanol or water and vegetable edible oil without using harmful organic solvent at the time of extraction and finally removing substantially the water-soluble solvent .

In addition, the method for producing licorice extract of the present invention is advantageous in that it is easy to apply to mass production since a complicated facility is not required by a relatively simple process.

According to an aspect of the present invention, there is provided a composition for preventing or treating oral diseases, which comprises the licorice extract obtained as described above as an active ingredient.

The licorice extract obtained by the above-mentioned process has an MIC ₉₀ value of about 100 μg / mL or less, about ₉₀ μg / mL or less, or about 80 μg / mL or less for S. mutans, , And it contains little glycyrrhizin and is not toxic to human body.

According to one embodiment of the present invention, the licorice extract obtained according to the production method of the present invention may exhibit an effect of reducing the concentration (ppm) of glycyrrhizin by about 95% or more, preferably by about 97% or more.

According to one embodiment of the present invention, the composition for preventing or treating oral diseases comprising the licorice extract as an active ingredient may contain the licorice extract at a concentration of about 1 to about 100 μg / mL, no.

The composition for preventing or treating an oral disease of the present invention may include at least one selected from the group consisting of dental caries, periodontal disease, but is not limited thereto.

According to one embodiment of the present invention, the composition for preventing or treating oral diseases comprising the licorice extract as an active ingredient may be a pharmaceutical composition. When the composition for preventing or treating oral diseases is used for medicines, medicines or pharmaceutical uses, the licorice extract may be contained alone as an active ingredient, or may be pharmacologically acceptable depending on the composition of the composition, Acceptable carriers, excipients, diluents, other active ingredients or subcomponents.

According to another embodiment of the present invention, the composition for preventing or treating an oral disease comprising the licorice extract as an active ingredient may be any one selected from the group consisting of a toothpaste, a mouthwash, a mouthwash, and an oral spray. When the composition for preventing or treating oral diseases of the present invention is a formulation of toothpaste, it may be prepared according to a method generally used in the technical field of the present invention, and may be selected from the group consisting of abrasives, wetting agents, binders, foaming agents, sweeteners, , A pharmaceutical active ingredient, a flavoring, a brightening agent, a pigment, a solvent, and the like.

According to another embodiment of the present invention, the composition for preventing or treating oral diseases comprising the licorice extract as an active ingredient may be a food additive.

When the composition of the present invention is used as a food additive, the licorice extract may be added to the food or beverage as it is or in combination with other food additives. When the licorice additive of the present invention is added at the time of preparation of a food or beverage, the amount thereof is not particularly limited and may be used in an appropriate amount within a range that does not cause any safety problems.

In addition, the above food or beverage includes all kinds of general preference foods, health functional foods, health supplements, various beverages, functional beverages and the like, and examples thereof include chocolate, candy, jelly, snack, confectionery, , Gums, ice cream, beverage, tea, drink, or alcoholic drinks, but the present invention is not limited thereto.

Best Mode for Carrying Out the Invention Hereinafter, the function and effect of the present invention will be described in more detail through specific examples of the present invention. It is to be understood, however, that these embodiments are merely illustrative of the invention and are not intended to limit the scope of the invention.

<Examples>

Example 1

100 g of licorice powder (agave food sale, domestic licorice powder) and 800 mL of ethanol were put in a glass bottle, mixed well, intermittently stirred, and left at room temperature for 72 hours. The mixture was filtered three times with Whatman filter paper to completely remove the licorice powder and obtain only the ethanol extract of licorice (step 1).

150 mL of the thus obtained ethanol extract was transferred into a new bottle. 200 mL of grape seed oil (supplier: Target Chung Jung Won) was added to the mixture, and the mixture was thoroughly mixed and allowed to stand at room temperature for layer separation. After 24 hours, only the oil layer was transferred to a new bottle with the oil layer and the ethanol layer separated (Step 2).

To transfer the functional material from the separated oil layer to the ethanol layer again, add 150 mL of fresh ethanol to the oil layer, mix thoroughly, and allow to stand for 12 hours at room temperature. Separated into an oil layer and an ethanol layer, only the ethanol layer was carefully transferred into a new bottle (A, step 3, first extraction).

150 mL of fresh ethanol was added to the oil layer from which the ethanol layer was separated, and the mixture was thoroughly mixed. Only the ethanol layer was carefully transferred to the bottle (A) in the state of being separated into the oil layer and the ethanol layer (third step, second extraction).

100 mL of fresh ethanol was added to the oil layer from which the ethanol layer was separated, and the mixture was thoroughly mixed. After the layer separation of the oil layer and the ethanol layer was confirmed, only the ethanol layer was carefully transferred to the bottle (A) to obtain an ethanol extract extracted three times in total (third step, third extraction).

Ethanol was removed from the ethanol extract of the bottle (A) using a vacuum rotary condenser, and further left in a high vacuum for 24 hours to completely remove the remaining ethanol to obtain a final licorice extract (Step 4).

Example 2

The licorice extract was obtained in the same manner as in Example 1 except that canola oil (CJ Cheiljedang Co., Ltd.) was used instead of grape seed oil as an oil.

Example 2

Licorice extract was obtained in the same manner as in Example 1, except that olive oil (Filippo Berio) was used instead of grape seed oil as an oil.

Comparative Example 1

100 g of licorice powder (agave food sale, domestic licorice powder) and 800 mL of ethanol were added to a glass bottle, mixed well, intermittently stirred, and left at room temperature for 72 hours. The mixture was filtered three times with Whatman filter paper to completely remove the licorice powder and obtain an ethanol extract of licorice root. The remaining ethanol was removed using a vacuum rotary condenser, and left in a high vacuum for 24 hours to obtain a licorice extract.

<Experimental Example>

1) Measurement of Minimum Inhibitory Concentration (MIC)

MIC was measured by micro-dilution assay according to National Committee for Clinical Laboratory Standards.

More specifically, S. mutans (ATCC 25175) was cultured in TSB (Trypticase Soy Broth) medium at 37 ° C. In order to examine the effect of DMSO used as a solvent on S. mutans, 10 ⁴ S. mutans bacteria per well were added to a 96-well plate, and final concentrations of 0.5%, 1.0%, 1.5%, 2.0% 2.5% 3.0% DMSO was added. Three wells were used per concentration of DMSO. The positive control group was used in the absence of DMSO and the negative control group was used in the absence of S. mutans. After incubation at 37 ° C for 16 hours, OD ₆₀₀ , the optical density value at 600 nm, was measured.

The inhibition of growth of S. mutans by DMSO concentration did not affect the growth of S. mutans until 2% DMSO concentration. Based on these preliminary experimental results, the final DMSO concentration per well in the MIC values of the extracts of Examples 1 to 3 and Comparative Example 1 was adjusted to 1.0%.

For MIC measurement, the extracts of Examples 1 to 3 and Comparative Example 1 were completely dissolved in DMSO to a concentration of 40 mg / mL. The extract was added so that final concentrations per well were 400, 200, 100, 50, 25, 12.5, 6.25, and 3.125 μg / mL. Three wells were used for each concentration.

10 ⁴ S. mutans bacteria were added to each well except the negative control, and the OD ₆₀₀ was measured after culturing at 37 ° C for 16 hours. The value for inhibiting the growth of S. mutans by 90% is calculated by MIC ₉₀ and is shown in Table 1 below. In addition, the graph is shown in FIG. 1, in which the MIC ₉₀ value is connected to the S curve using SigmaPlot 13.0 from Systat.

MIC experiments were performed using 3 wells per concentration and 3 independent measurements. To examine the statistical significance of the MIC values, one-way ANOVA analysis was performed using Prism 6.0 from Graphpad. The Dunnett's multiple comparisons test was performed to verify the difference between the MIC values of Comparative Example 1 and Examples 1 to 3, Respectively.

Example 1 Example 2 Example 3 Comparative Example 1 MIC ₉₀ 52.9 99.7 86.3 123.9

* In Table 1, the unit of MIC ₉₀ is μg / mL.

Referring to Table 1 and FIG. 1, it can be seen that the R 2 values are all 0.99 or more and are well bonded to the S curve. MIC ₉₀ value was the lowest in Example 1 extracted with grape seed oil, and Comparative Example 1 extracted with ethanol alone was the highest.

Also, referring to FIG. 2, the MIC ₉₀ value was statistically significant at P <0.01 when Example 1 and Comparative Example 1 were compared.

2) Confirming the inhibitory effect of grape seed oil on S. mutans

In Example 1, it was confirmed whether the specific components of the grape seed oil used for the extraction were transferred to the final extract in the course of re-extraction with ethanol to inhibit the growth of S. mutans. For this purpose, in Example 1, the third step was repeated three times using ethanol for grape seed oil without licorice powder, and the solvent was removed. After the solvent was removed, the S. mutans strain The growth inhibitory effect was confirmed and the results are shown in Fig.

As a result, the substance obtained by extracting only grape seed oil with ethanol showed almost no inhibition of the growth of S. mutans even at a maximum concentration of 400 μg / mL, and the inhibitory effect of Example 1 against S. mutans was due to the licorice extract Respectively.

3) Glycyrrhizin removal effect

The content of glycyrrhizin was analyzed by HPLC on the licorice powder before extraction used in Example 1 and the final licorice extract extracted by Example 1, respectively. As a result of analysis, 9,320 ppm of glycyrrhizin was present in the licorice powder before extraction, but 218 ppm was contained in the licorice extract extracted by the extraction method of Example 1, showing a reduction rate of about 97.7%.

Claims (11)

Mixing the licorice and the first water-soluble solvent to obtain a first water-soluble extract;
A second step of mixing the first aqueous extract and the edible oil to obtain an oil extract;
A third step of mixing the oil extract and the second water-soluble solvent to obtain a second water-soluble extract; And
And a fourth step of removing the second water-soluble solvent from the second water-soluble extract to obtain a licorice extract.
The method according to claim 1,
Wherein the first and second water-soluble solvents are the same or different from each other and are water, a lower alcohol having 4 or less carbon atoms, or a mixture thereof.
The method according to claim 1,
Wherein the second step is repeated twice or more.
The method according to claim 1,
Wherein the third step is repeated two or more times.
The method according to claim 1,
Wherein the edible oil comprises at least one selected from the group consisting of grape seed oil, canola oil, olive oil, safflower seed oil, sunflower seed oil, corn oil, soybean oil, and perilla oil.
The method according to claim 1,
Wherein the content of glycyrrhizin is reduced by 95% or more by weight, compared with the licorice before extraction.
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