KR101798219B1 - Imaging Device and Method for Obtaining Multi Contrast Images - Google Patents

Abstract

An aspect of the present invention relates to an image information acquiring apparatus and method for securing a multimode image, and more particularly, to an image information acquiring apparatus and an image information acquiring apparatus capable of acquiring a plurality of multimode images at a time using a color- And a method thereof.
According to an embodiment of the present invention, a light source or a filter capable of irradiating a color pattern at low cost instead of a specialized condenser and an objective lens can be used to obtain bright field, dark field and optical phase difference image information in one shot Provides an effect.

Description

Technical Field [0001] The present invention relates to an apparatus and a method for acquiring a multi-

An aspect of the present invention relates to an image information acquiring apparatus and method for securing a multimode image, and more particularly, to an image information acquiring apparatus and an image information acquiring apparatus capable of acquiring a plurality of multimode images at a time using a color- And a method thereof.

The contents described in this section merely provide background information on the embodiment of the present invention and do not constitute the prior art.

1 (a) shows a bright field image, FIG. 1 (b) shows a dark field image, and FIG. 1 (c) shows an optical phase difference image.

Generally, a bright field image is an image obtained by observing how weak a beam is as it passes through the subject. Observation of a living tissue such as a cell with a microscope is difficult to obtain images through focusing, and can be secured through defocusing .

Since a living tissue such as a cell is difficult to obtain an image by a general microscope, it is possible to secure an image through dyeing or the like, or to provide a dark contrast image or a differential interference phase difference (DIC ) Image or the like.

Conventional image acquisition devices such as microscopes can only acquire bright field images. In order to obtain differential interference phase difference (DIC) images or dark-field images, additional optical systems such as specialized objective lenses or condensers must be equipped.

The time and expense of these additional equipment increases. Therefore, it is urgent to develop an image information acquiring apparatus or method capable of obtaining bright field, dark field, and optical phase difference image information by using a light source or a filter capable of low-cost color pattern irradiation.

Korean Unexamined Patent Publication No. 10-2010-0123256 "Dark night microscope and method for detecting scattered light using the same"

Accordingly, an aspect of the present invention has been proposed to solve the above-mentioned problems, and an object of the present invention is to provide a light source that can give different patterns according to colors, instead of a light source of a general microscope, And an image information acquiring apparatus and an image information acquiring method that can obtain an optical phase difference image by one shot.

Another object of the present invention is to provide an image information acquiring apparatus and an image information acquiring method which can obtain quantitative phase information by two photographings.

The technical object of the present invention is not limited to the above-mentioned technical objects and other technical objects which are not mentioned can be clearly understood by those skilled in the art from the following description will be.

According to an aspect of the present invention, there is provided an apparatus for acquiring image information, including a light emitting unit for irradiating a plurality of beams having different wavelengths to an object to be examined.

In addition, it is also possible to receive the plurality of beams passed through the subject, to separate color information from the plurality of received beams, to process the separated color information, And a light receiving unit for acquiring a plurality of different image information.

According to another aspect of the present invention, there is provided an apparatus for acquiring image information, including a light source for irradiating a beam with a pseudo body.

And a filter provided between the light source and the subject to form a pattern so that a plurality of different colors are imparted to the beam irradiated by the light source.

And a controller for receiving the plurality of beams passed through the subject and separating color information from the plurality of beams received and calculating the separated color information to generate a plurality of different image information about the subject And a light-receiving portion for securing the light-receiving portion.

According to still another aspect of the present invention, there is provided an apparatus for acquiring image information, including a first area for irradiating a beam having a first color, a second area for irradiating a beam having a second color, And a pattern portion including a third region for irradiating a beam having a third color.

The apparatus may further include an object support supporting the object to be inspected.

Receiving the plurality of beams accommodating information about the object to be inspected in cooperation with the object to be examined, separating color information from the plurality of received beams, arithmetically processing the separated color information, And a light receiving unit that acquires a plurality of different image information regarding the object to be inspected.

According to another aspect of the present invention, there is provided a method for acquiring image information, including the steps of: irradiating a first beam having a first color to an object to be inspected;

And a second irradiating step of irradiating a second beam having a second color different from the first color to the object to be inspected.

And the first beam and the second beam interact with the inspected object to receive information about the inspected object.

And a light receiving step of receiving the first beam and the second beam containing information on the object to be inspected.

And securing a plurality of different pieces of image information regarding the subject from the received first beam and the second beam.

As described above, according to the embodiment of the present invention, by using a light source or a filter capable of irradiating low-cost color patterns instead of a specialized condenser and an objective lens, bright field, dark field and optical phase difference Thereby providing an effect of obtaining image information.

In addition, it is possible to obtain the quantitative phase information of the subject by applying a specific algorithm to the optical phase difference image in one direction (X direction) and the optical phase difference image in the other direction (Y direction) different from the one direction to provide.

In addition, the effects of the present invention have various effects such as excellent durability according to the embodiments, and such effects can be clearly confirmed in the description of the embodiments described later.

Figure 1 shows a bright field image, a dark field image and an optical phase difference image.
2 shows an image information acquiring apparatus according to an embodiment of the present invention.
3 shows a region irradiated with a beam in the light emitting portion according to an embodiment of the present invention.
Fig. 4 shows quantitative phase image information of the subject obtained by two photographs.

Hereinafter, an embodiment of the present invention will be described in detail with reference to exemplary drawings.

It should be noted that, in adding reference numerals to the constituent elements of the drawings, the same constituent elements are denoted by the same reference numerals whenever possible, even if they are shown in different drawings. In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

In addition, the size and shape of the components shown in the drawings may be exaggerated for clarity and convenience of explanation. In addition, terms specifically defined in consideration of the constitution and operation of the present invention are only for explaining the embodiments of the present invention, and do not limit the scope of the present invention.

2 shows an image information acquiring apparatus according to an embodiment of the present invention.

The image information obtaining apparatus 100 according to an embodiment of the present invention includes a light emitting unit 110 for emitting a plurality of beams having different wavelengths to an object 120; And

(120), separates color information from the plurality of received beams, performs arithmetic processing on the separated color information, and outputs the color information to the subject And a light receiving unit 160 for acquiring a plurality of different image information. Here, the plurality of different image information may mean a bright field image, dark field image, and optical phase difference image with respect to the subject 120.

The image information obtaining apparatus 100 according to an embodiment of the present invention may include a condenser for condensing a beam irradiated from the light emitting unit 110 and irradiating the subject 120 between the light emitting unit 110 and the light receiving unit 160 . ≪ / RTI > The condenser may be a kind of lens that allows a plurality of beams emitted from the light emitting unit 110 to be irradiated to the subject 120 at different angles.

The image information obtaining apparatus 100 according to an embodiment of the present invention may include a support for supporting the subject 120 between the condenser and the light receiver 160. Between the support and the light receiver 160, A lens group composed of the object lens 130, the Fourier plane, and the tube lens 150 may be arranged as a configuration for adjusting the magnification, for example. Such a lens group may have other configurations for performing the same function.

The subject body 120 may mean a living body tissue such as a cell, but is not limited thereto, and any body including microstructure may be used.

The light emitting unit 110 may emit a plurality of beams having different wavelengths. Wherein the plurality of beams having different wavelengths may include each of the plurality of beams having a different color. However, since a plurality of beams each have a different color, it does not necessarily mean a visible ray.

According to an embodiment, the light emitting portion 110 may include an LED array. That is, the light emitting unit 110 may include a plurality of light emitting diode arrays each capable of emitting beams of various colors.

The light emitting unit 110 may be electrically connected to the control unit according to the embodiment, and the color of each beam irradiated from the light emitting diode array may be adjusted or changed through the control unit.

According to the embodiment, the light emitting portion 110 may be configured to include a light source for emitting a beam and a filter for transmitting only a beam of a specific wavelength or a beam having a specific range of wavelengths. In this case, the beam irradiated from the light source passes through the filter and becomes a plurality of beams having different wavelengths, and can be irradiated to the subject 120.

In an exemplary embodiment, the filter may be electrically or mechanically connected to the control unit, and the color pattern may be changed under the control of the control unit. In addition, a plurality of filters may be provided in the exemplary embodiment, and the plurality of filters may have various color patterns. A user can select a filter having a desired pattern among these filters and install the selected filter in the image information acquiring apparatus 100.

According to the embodiment, the light emitting portion 110 can irradiate a plurality of beams simultaneously or sequentially. Also, according to the embodiment, the light receiving unit 160 may include a color camera having a CCD.

The plurality of beams emitted from the light emitting unit 110 may include a first color and a second color having a different wavelength from the first color and may further include a third color having a different wavelength from the first color and the second color, And may include a fourth color having a different wavelength from the first color, the second color, and the third color. The plurality of beams irradiated from the light emitting portion 110 is not limited to the color range here.

The case where the light emitting unit 110 irradiates the beams of the first color, the second color and the third color will be described.

FIG. 3 shows a region for irradiating a beam in the light emitting portion 110 according to an embodiment of the present invention. 3 (a) shows a case where the boundary line between the first color region 210 and the second color region 220 is vertical, and Fig. 3 (b) shows a case where the boundary line is horizontal.

Fig. 4 shows quantitative phase image information of the subject obtained by two photographs.

According to the embodiment, the light emitting unit 110 may be arranged in a predetermined pattern with a region for irradiating the beam of the first color, the second color, and the third color.

Depending on the embodiment, the first color may include blue, the second color may include red, and the third color may include green. For example, the first color may be blue, the second color may be red, and the third color may be green. In this case, when the light receiving unit 160 is a color camera capable of recognizing blue, red, and green beams, a plurality of beams can be received and directly separated into blue, red, and green. That is, the color information stored in the beam can be separated at a low cost.

According to the embodiment, the pattern may be such that the first color and the second color are disposed in contact with each other, and the third color is disposed so as to surround the first color and the second color. According to the embodiment, the first color and the second color may be formed in a semicircular shape in which straight portions of each other are in contact with each other.

The areas 210 and 220 of the first color and the second color surrounded by the third color may be formed to correspond to the numerical aperture of the object lens 130 described above. That is, since the shape of the object lens 130 is circular, the region 230 surrounded by the third color, that is, the regions 210 and 220 of the first color and the second color, can also be formed in a circular shape.

The beams irradiated in the first color region 210 and the second color region 220 may be converged through the condenser and then passed through the roll of the object 120 to be irradiated to the object lens 130. Meanwhile, since the third color region 230 is disposed surrounding the first and second color regions 210 and 220, the beam of the third color is located at the outer periphery of the numerical aperture of the object lens 130.

The case where the light emitting unit 110 is a light emitting diode array and the light receiving unit 160 is a color camera will be described as an example in the image information obtaining apparatus 100 according to an embodiment of the present invention.

The light emitting diode array included in the light emitting unit 110 may be located on a Fourier plane 140 of the subject 120 to be photographed. Here, since each light emitting diode on the array can be individually controlled, a pattern as shown in FIG. 3 can be formed. When the pattern of the light source is formed as shown in FIG. 3 (b), the beam irradiated in the blue region 210 at the upper portion and the beam 220 irradiated in the red region 220 at the lower portion are incident on the subject 120 at different angles The beam that has been irradiated and passed through the subject 120 passes through the objective lens 130, the Fourier plane 140, and the tube lens 150, and is received through the CCD of the color camera.

In this case, if the color camera separates the color information of the red beam and the blue beam, subtracts them from each other, and divides them by adding the color information of the red beam and the color information of the blue beam, A differential phase difference image can be obtained. In other words, in the embodiment, the following equation (1) is expressed by the color information (R) of the red beam - the color information (B) of the blue beam / (the color information (R) , (RB) / (R + B), and the Y-direction differential phase difference image can be obtained.

At this time, if the color camera separates the color information of the red beam and the blue beam and divides them by the sum of the values, the Y-directional optical phase difference image can be obtained.

In addition, by adding the color information of the blue beam and the color information of the red beam, a general bright field microscope image can be obtained.

Since the green beam is at the outer periphery of the numerical aperture of the object lens 130, the color information of the green beam may have information scattered by the subject 120. Therefore, if only the color information of the green beam is separated, the dark image can be obtained. That is, the bright field, the dark field, and the differential phase difference image can be obtained in one shot.

Meanwhile, the light receiving unit 160 acquires the first information in a state where the boundary line 240 in which the two straight lines are tangent is formed in one direction, and the second information is formed in a state in which the boundary line 240 is formed in the other direction perpendicular to the one direction And acquire the image information based on the first and second information after acquisition.

Here, the one direction and the other direction may be perpendicular to each other, and may refer to the Y axis direction and the X axis direction, respectively (see FIG. 4). In other words, one direction may be a pattern of a light source as shown in FIG. 3 (b) where a blue region 210 is formed at the upper part and a boundary line 240 where both straight lines of the red region 220 at the lower side are in contact with each other is formed horizontally , And the other direction may be a case where the boundary line 240 between both the right side red region 220 and the pale green region 210 on the left side is perpendicular to the left side region 210 as shown in FIG. 3 (a).

Specifically, if the light emitting diode array is shaped as shown in FIG. 3 (a), and the values obtained by subtracting the color information of the red beam and the blue beam are added to each other, an optical phase difference image in the X direction can be obtained. Here, the optical phase difference image in the X direction may correspond to the first information described above.

In addition, if the light emitting diode array is shaped as shown in FIG. 3 (b) and the above process is repeated, a Y-direction optical phase difference image can be obtained. Here, the optical phase difference image in the Y direction may correspond to the second information described above.

Quantitative phase information of the subject 120 can be obtained by applying the Complex Fourier Integral algorithm to the optical phase difference images in the X direction and the Y direction obtained through the two photographing operations.

The apparatus 100 for acquiring image information according to another embodiment of the present invention includes a light source for irradiating a beam with a pseudo body, A filter for forming a pattern such that a plurality of different colors are imparted to the beam irradiated by the light source; And

(120), separates color information from the plurality of received beams, performs arithmetic processing on the separated color information, and outputs the color information to the subject And a light receiving unit 160 for acquiring a plurality of different image information.

According to the embodiment, a filter may be provided between the light source and the subject 120. However, it is not necessarily required to be in that position, and the filter may be located on the Fourier plane of the subject 120 according to the embodiment. In this case, the beam irradiated from the light source can pass through the filter on the Fourier plane encountered through the object 120. When an object lens is added according to the embodiment, the beam irradiated from the light source can meet the filter on the Fourier plane, which is encountered after passing through the object and the object lens.

The apparatus 100 for acquiring image information according to an embodiment of the present invention may include a controller for changing and controlling a pattern of a filter.

According to another embodiment, the filter may be a plurality of filters, and the plurality of filters may have different patterns. In this case, the user can select a filter having a desired pattern among a plurality of filters, and install the selected filter in the image information obtaining apparatus 100.

The image information obtaining apparatus 100 according to another embodiment of the present invention includes a first region 210 for irradiating a beam having a first color, a second region 220 for irradiating a beam having a second color, A pattern portion 110 including a third region 230 for irradiating a beam having a third color; An object support supporting the object to be inspected 120; And

Receiving the plurality of beams that receive information about the subject 120 by interacting with the subject 120, separating color information from the plurality of received beams, And a light receiving unit 160 for computing a plurality of different image information regarding the subject 120 by processing information.

Here, the pattern unit 110 may correspond to the light emitting unit 110 in the above-described embodiment. The first region 210 and the second region 220 are disposed adjacent to each other and the third region 230 surrounds the first region 210 and the second region 220 .

The light receiving unit 160 secures the first information in a state in which the boundary line 240 in which the first area 210 and the second area 220 are in contact with each other is formed in one direction and the boundary line 240 is formed in the other direction perpendicular to the one direction The image information can be secured through the first information and the second information after securing the second information.

Here, the first information and the second information may correspond to the optical phase difference images in the X direction and the Y direction, respectively.

The pattern unit 110 may include a fourth region for irradiating a beam having a first color, a second color, and a fourth color different from the third color. In this case, for example, the first color may be blue, the second color may be red, the third color may be green, and the fourth color may be yellow. In this case, the light receiving unit 160 may include a four-color camera capable of recognizing and separating blue, red, green, and yellow. In this case, the shape of the pattern may be, for example, a shape of a quadrant in which the plane is divided into four portions, and a beam of blue, red, green, and yellow is applied to each of the first, second, third and fourth quadrants The area to be irradiated may be disposed.

 According to another embodiment of the present invention, there is provided a method of acquiring image information, comprising: a first irradiating step of irradiating a subject 120 with a first beam having a first color; A second irradiation step of irradiating a second beam having a second color different from the first color to the object 120;

The first beam and the second beam interacting with the subject 120 to receive information about the subject 120; A light receiving step of receiving the first beam and the second beam containing information on the object to be inspected 120; And

And securing a plurality of different pieces of image information regarding the subject 120 from the received first beam and the second beam.

In the first irradiation step and the second irradiation step, the first beam and the second beam can be irradiated to the object 120 at different angles.

In the first irradiation step and the second irradiation step according to the embodiment, the first beam and the second beam can be irradiated to the object 120 at the same time. According to the embodiment, in the light receiving step, the first beam and the second beam can be received at the same time.

According to an embodiment, the step of securing information may include extracting first information of the first beam and second information of the second beam, and computing the first information and the second information.

The above description is only illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention.

The embodiments disclosed in the present invention are not intended to limit the scope of the present invention and are not intended to limit the scope of the present invention.

The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

100: Acquisition device
110: light emitting portion, pattern portion
120: Subject
130: object lens
140: Fourier plane
150: tube lens
160:
210: blue area
220: Red area
230: green area
240: Border

Claims (27)

A light emitting unit for irradiating a plurality of beams having different wavelengths to the object to be inspected; And
A plurality of beams of light having passed through the object to be inspected are received, color information is separated from the plurality of received beams, the separated color information is arithmetically processed, and a plurality of And a light receiving unit for acquiring different image information,
Wherein the plurality of beams of the light emitting portion are composed of a first color, a second color having a different wavelength from the first color, and a third color having a different wavelength from the first and second colors,
Wherein the first color area, the second color area, and the third color area are arranged in a predetermined pattern. The method according to claim 1,
Wherein the light emitting unit includes an LED array. The method according to claim 1,
Wherein the light emitting unit includes a light source that emits a beam and a filter that transmits only a beam of a specific wavelength or a beam of a specific range of wavelengths. The method according to claim 1,
Wherein the light emitting unit emits the plurality of beams at the same time, The method according to claim 1,
Wherein the light emitting unit and the light receiving unit are disposed with the subject to be interposed therebetween. The method according to claim 1,
Wherein the light receiving unit comprises a color camera. The method according to claim 1,
And a condenser provided between the light emitting unit and the subject to irradiate the subject with the beams at different angles. delete The method according to claim 1,
Wherein the first color includes blue, the second color includes red, and the third color includes green.
delete The method according to claim 1,
Wherein the pattern is a pattern in which the region of the first color and the region of the second color are disposed in contact with each other and the region of the third color is disposed so as to surround the first color and the second color Image information acquisition device. The method according to claim 1,
An object lens is disposed between the subject and the light receiving unit, and the first and second color areas surrounded by the third color area correspond to a numerical aperture of the object lens. Image information acquisition device. The method according to claim 1,
Wherein the region of the first color and the region of the second color are formed in a semicircular shape in which straight portions of each other are in contact with each other. 14. The method of claim 13,
Wherein the light receiving unit obtains the first information in a state in which the boundary line between the both straight lines is formed in one direction and the second information is acquired in a state where the boundary line is formed in the other direction perpendicular to the one direction, And acquires the image information based on the acquired image information. 12. The method of claim 11,
And a control unit electrically or mechanically connected to the light emitting unit to change and control the shape of the pattern. A light source for irradiating the beam with a pseudo body;
A filter which is provided between the light source and the object to be inspected so that a first color region having a plurality of different colors and a second color region and a third color region are arranged in a predetermined pattern in a beam irradiated by the light source, ; And
A plurality of beams of light having passed through the object to be inspected are received, color information is separated from the plurality of received beams, the separated color information is arithmetically processed, and a plurality of A light receiving unit for acquiring different image information;
The image information acquiring device comprising: 17. The method of claim 16,
And a control unit for changing and controlling the pattern of the filter. 17. The method of claim 16,
Wherein the plurality of filters are formed in a plurality of different patterns, respectively. A pattern portion including a first region for irradiating a beam having a first color, a second region for irradiating a beam having a second color, and a third region for irradiating a beam having a third color;
An object support supporting the object to be inspected; And
Receiving the plurality of beams accommodating information about the object to be inspected in cooperation with the object to be inspected, separating color information from the plurality of received beams, arithmetically processing the separated color information A light receiving unit for acquiring a plurality of different image information about the subject in a photographing operation;
The image information acquiring device comprising: 20. The method of claim 19,
Wherein the pattern unit includes a fourth area for irradiating a beam having a fourth color different from the first color, the second color, and the third color. 20. The method of claim 19,
Wherein the pattern unit is disposed such that the first area and the second area are in contact with each other, and the third area is disposed so as to surround the first area and the second area. 22. The method of claim 21,
The light receiving unit secures the first information in a state that the boundary line between the first area and the second area is formed in one direction and secures the second information in a state where the boundary line is formed in the other direction perpendicular to the one direction And secures the image information through the first information and the second information. A first irradiation step of irradiating a first beam having a first color to an object to be inspected;
A second irradiation step of irradiating a second beam having a second color different from the first color to the object to be inspected;
The first beam and the second beam interacting with the subject to receive information about the subject;
A light receiving step of receiving the first beam and the second beam containing information on the object to be inspected; And
And securing a plurality of different pieces of image information relating to the subject in one shot from the received first beam and the second beam,
Wherein the step of securing information comprises the steps of separately extracting first information of the first beam and second information of the second beam and calculating the first information and the second information, Image information acquisition method. 24. The method of claim 23,
Wherein the first beam and the second beam are irradiated to the subject at different angles in the first irradiation step and the second irradiation step. 24. The method of claim 23,
Wherein the first beam and the second beam are irradiated to the object simultaneously at the first irradiation step and the second irradiation step. 24. The method of claim 23,
Wherein the first beam and the second beam are received at the same time in the light receiving step. delete

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