KR101795147B1 - The whitening composition containing Mulberry seed extract and the extracting method - Google Patents
The whitening composition containing Mulberry seed extract and the extracting method Download PDFInfo
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- KR101795147B1 KR101795147B1 KR1020150131234A KR20150131234A KR101795147B1 KR 101795147 B1 KR101795147 B1 KR 101795147B1 KR 1020150131234 A KR1020150131234 A KR 1020150131234A KR 20150131234 A KR20150131234 A KR 20150131234A KR 101795147 B1 KR101795147 B1 KR 101795147B1
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- hexane
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- skin whitening
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
Abstract
The present invention relates to a composition for skin whitening comprising an extract of Odyssey as an active ingredient and a method for extracting an effective ingredient thereof.
The odd ethanol extract, the lower hexane fraction and the ethyl acetate fraction according to the present invention have excellent skin whitening effect, and thus can be utilized as a skin whitening cosmetic composition.
Description
The present invention relates to a composition for skin whitening comprising an extract of Odyssey as an active ingredient and a method for extracting an effective ingredient thereof.
The present inventors have experimentally confirmed that an extract of Odyssey ethanol has skin whitening activity by inhibiting melanin synthesis. Higher whitening activity was also observed in the hexane fraction, ethylacetate fraction and the like of the lower isolates.
In conclusion, the extract of Oddi-ethanol, the sub-hexane fraction and the ethyl acetate fraction are excellent in skin whitening effect and thus can be used as a skin whitening cosmetic composition.
Mulberry which is the subject of the present invention is a fruit of Morus alba L. and is collected from May to June when the fruit shows black or magenta and is used by being edible or dried. In one room, it has been used as a tonic, a nurse that darkens gray hair, relieves sorghum (diabetes), and benefits the five.
Although many studies on physiological activity using such an audi have been carried out, there is little research on Audi. In recent years, polyphenol compounds, which are antioxidant, anticancer and anti-diabetic components, have been isolated from Audi seeds, but the ability of Audi to inhibit melanin synthesis has not been reported yet.
The present inventors have explored the possibility of a composition for skin whitening of Odyssey. As a result, it was confirmed that the skin extract of Oddi, the subhexane fraction and the ethyl acetate fraction excelled in the skin whitening effect, leading to the present invention.
An object of the present invention is to detect and realize the possibility of a composition for skin whitening of Odyssey.
And further to provide a method for optimally obtaining a composition for skin whitening in Audi seeds.
The skin whitening composition of the present invention as described above is characterized by containing an extract of Odyssey as an active ingredient.
At this time, the Odidi seed extract is an ethanol extract.
The present invention also provides a skin whitening composition comprising at least one of a lower hexane fraction, an ethyl acetate fraction and a butanol fraction of an aqueous extract of Odyssey as an active ingredient.
And an ethyl acetate fraction derived from an olive oil as an active ingredient.
The method for extracting an extract of Odyssey for skin whitening according to the present invention comprises:
(1) extracting Odyssey with ethanol;
(2) adding hexane to the supernatant obtained in (1) to extract;
(3) Extracting the distilled water layer excluding the hexane fraction from the extract of (2) with ethyl acetate; And
(4) recovering only the ethyl acetate fraction from the fraction obtained in the above (3) except for the distilled water layer to obtain the target ethyl acetate fraction.
(1) extracting Odyssey with ethanol;
(2) adding hexane to the supernatant obtained in (1) to extract;
(3) Extracting the distilled water layer excluding the hexane fraction from the extract of (2) with ethyl acetate;
(4) Extracting the distilled water layer except the ethyl acetate fraction in the extract of (3) with butanol; And
(5) recovering only the butanol fraction from the fraction obtained in the above (4) except for the distilled water layer to obtain the desired hexane fraction and butanol fraction.
The present invention is also a skin whitening composition comprising, as an active ingredient, a hexane fraction, an ethyl acetate fraction or a butanol fraction obtained by the above extraction method.
The oedic ethanol extract of the present invention has skin whitening activity by inhibiting melanin synthesis.
In addition, the lower separations, such as hexane fraction and ethylacetate fraction, have high whitening activity, and the ethyl acetate fraction shows the best whitening effect.
Therefore, it can be widely used as a skin external preparation for skin such as a cosmetic composition.
1 is a view showing an extraction process of an Audi seed according to the present invention.
Hereinafter, the present invention will be described in detail with reference to examples.
Example 1: Extraction of materials using Odyssey
Odd seed (500 g) was immersed in ethanol, sonicated and separated, and centrifuged to separate the supernatant. This procedure was repeated three times to obtain an ethanol extract.
For the material fraction, the ethanol extract is suspended in water.
After that, hexane fraction (HA fr.) Is obtained by shaking the same amount of hexane and recovering only the hexane fraction.
The remaining distilled water layer is shaken again with the same amount of ethylacetate, and only the ehthylacetate fraction is recovered to obtain the ethylacetate fraction (EA fr.).
The remaining distilled water layer is extracted by shaking with butanol in the same manner as above, and only the butanol fraction is recovered to obtain a butanol fraction (BuOH fr.).
Through this process, the four kinds of fractions were separated as shown in FIG. 1 up to the remaining distilled water layer.
Solvents were removed from each of the four fractions and lyophilized to obtain experimental powders.
Example 2: Assessment of Melanin synthesis inhibitory effect of Odidi seed extract
The inhibition of melanin synthesis was analyzed by using an extract of Odidi (Fig. 1).
First, melan-a cells, immortalized cell lines derived from C57BL / 6 mice, were used. Melan-a cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum, streptomycin-penicillin (100 mg / mL) and 200 nM 12- ο- tetradecanoylphorbol-13- 2 cells were cultured and used.
Specific experimental methods for inhibiting the biosynthesis of melanin were 1 × 10 5 Melan-a cells in each well of a 24-well plate and cultured overnight. Each sample was treated with the concentration by concentration and cultured for 4 days. After washing with phosphate-buffered saline, 250 μL of 0.85N KOH was dissolved in each well. Transfer the lysed cells to a 96-well plate and measure the optical density at 405 nm using a microplate reader (Bio-Rad 3550). The inhibition rate of melanin production is calculated as% of the control.
Fig. As shown in Fig. 1, the ethanolic ethanol extract inhibited the melanin synthesis of melan-a cells by 57% and 68% at the concentrations of 20 and 40 μg / ml, respectively. In the first fraction, the cells treated with hexane fraction and ethylacetate fraction of 40 μg / ml showed cell cytotoxicity, indicating that the cells did not show correct data. However, the hexane fraction and ethylacetate fraction of 20 μg / ml showed high melanin inhibition It looked.
Example 3: Measurement of tyrosinase inhibitory activity of the extract
The mushroom tyrosinase inhibitory activity was analyzed by using an extract of Odidi (Fig. 2).
Tyrosinase inhibition activity was determined by adding 150 μl of 0.1 M phosphate buffer (pH 6.5), 25 μl of 1.5 mM L-tyrosine solution and 2100 units / μl mushroom tyrosinase (Sigma, 0.05 M phosphate buffer, pH 6.5) was added, and 15 μl of the sample was treated. After reacting at 25 ° C for 1 hour, the optical density was measured at 490 nm using a microplate reader (Bio-Rad 3550). The inhibition rate (%) for tyrosinase was calculated by the following equation.
As a result, 11% ethanolic extract of Oddi was inhibited. In the lower fraction, 18%, 80% and 31% of hexane fraction, ethylacetate fraction and butanol fraction were active, respectively.
Example 4: Experiment on inhibition of melanin synthesis of zebrafish ethanol extract of ODI
The inhibition of the synthesis of zebrafish melanin was carried out by using an ethanol extract of Audi. Mature zebrafish male and female eggs were collected in the sampling tank for sampling the day before harvest and eggs were collected 1-2 hours after the photoperiod of the next day. The harvested eggs were placed in zebrafish embryo medium for 24 hours, and each sample was treated by concentration.
Since the degree of permeation of the chorion sample was not known at the time of the sample treatment, it was treated by perforating the corions of each egg. After 24 hours after the sample treatment, the eggs showing toxicity of the sample were removed, The second sample was processed after complete removal. After 48 hours of the sample treatment, the degree of pigmentation of the sample treated with the control was observed with a stereomicroscope.
As a result, Fig. 3, the pigmentation of yolk part of the zebrafish treated with 10 μg / ml of the ODD ethanol extract was inhibited and the synthesis of melanin as a whole was inhibited as compared with the vehicle without the sample treatment Respectively.
As described above, the present invention detects and demonstrates the possibility as a composition for skin whitening of Odyssey. The present invention also provides a method for optimally obtaining an oily extract for skin whitening having the above-mentioned effects.
According to the results of the above experiment, the extract of Odyssey according to the present invention can be variously used as a skin external preparation for skin such as a cosmetic composition.
Claims (9)
(1) extracting Odyssey with ethanol;
(2) adding hexane to the supernatant obtained in the above (1) and extracting to obtain a hexane fraction;
(3) Extracting the distilled water layer excluding the hexane fraction in the extract of (2) with ethyl acetate;
(4) extracting the distilled water layer except the ethyl acetate fraction in the extract of (3) with butanol; And
(5) recovering only the butanol fraction from the fraction obtained in the above (4) except for the distilled water layer to obtain the target hexane fraction (= the hexane fraction of step (2)) and the butanol fraction.
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| KR101795147B1 true KR101795147B1 (en) | 2017-12-01 |
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| CN113797126A (en) | 2020-06-16 | 2021-12-17 | 玫琳凯有限公司 | Cosmetic composition |
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| Food Chemistry,제131권,2호,617-625면 (2011.09.17.)* |
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