KR101794294B1 - Compostion for immuno enhancement containing phytoncide - Google Patents

Abstract

The present invention relates to a composition capable of exhibiting an immunopotentiating effect even in transdermal administration, and is characterized by containing phytoncide, a base oil and azone as an active ingredient. It is also possible to provide a relatively immunosuppressive It shows excellent effect as a high-efficiency immunity enhancer to low-normal people, and also has an excellent effect as an immunity enhancer in chemotherapy.

Description

[0001] The present invention relates to a composition for enhancing immunity comprising phytoncide,

The present invention relates to a composition for improving immunity, particularly a composition capable of showing an immunostimulating effect even when transdermal administration.

Vertebrate animals have nonadaptive defenses against foreign invaders and foreign substances. There are skin tissues, defense cells and defense proteins that have non-adaptive defense functions in living body.

Defensive cells include phagocytes, which bind to all the pathogens that enter the body and ingest them and break them down. In addition, natural killer cells (NK cells) have the ability to detect and rapidly remove parasites or cancer cells.

Also, bacteria invaded from the outside. In order to protect itself from viruses, parasites or transformed cells that originate within itself, that is, against cancer cells or from toxins coming in from the outside (these are called "foreign substances"), Indicates an immune response.

Immune responses are observed only in vertebrate animals. Vertebrate animals show two types of immune responses. That is, the protein is destroyed by an antibody-mediated immune immune reaction or by a cell-mediated immune reaction. These immune responses are called specific immune responses.

The adaptive immune response with specificity is an acquired function of the living environment. AIDS is an acronym for acquired immune-deficient and is a disease that lacks immune function and can not defend the human body.

Two kinds of immunity that control immunity, cell and body fluids, arise from two types of lymphoid cells, which are not distinguished from each other. In other words, cells that determine their properties in both body fluids and cellular immune responses are termed lymphocytes. These cells are spread throughout the body and can travel through tissue cells, circulate through blood or lymph, and temporarily accumulate in lymphatic organs such as the spleen or lymph nodes.

According to the clonal selection hypothesis proposed by Burnet in 1950, each lymphoid site is differentiated into B-lymphocyte and T-lymphocyte according to its function as an immune cell. do. B-lymphocytes grow in the bone marrow in the case of mammals or in Fabricus vursa in the case of birds and T-lymphocytes grow in the thymus.

A more advanced understanding of the response of the immune system to cancer, AIDS, radioactivity and most other diseases in recent years has allowed us to explain the host defense mechanisms in a variety of ways with a broader understanding and a wider analysis.

On the other hand, the mechanism that directly acts on the tumor cells to cause growth inhibition or death, to strengthen the immune response of the living body, or to act on intermediates that activate the defense system, thereby increasing the ability of the host to defend against such diseases, In addition to enhancing susceptibility to other host immune systems, it can be used to reduce the mutation of mutant cells by acting on tumor cells or heterologous cells, or by promoting undifferentiated tumor cells to produce mature cells that do not divide And the mechanism of increasing the tumor treatment effect by alleviating the damage to the cytotoxicity of the living body by the chemotherapy and radiation therapy for the tumor. Unlike synthetic chemotherapeutic agents, these substances are called biologic response modifiers (BRMs), which affect the biological response of the body to inhibit cancer and reduce side effects And the effect can be increased.

In addition, it can be expected that the immunosuppressive effect exerts a good effect on AIDS and radiation exposure.

Although a number of compositions showing such immunity enhancing effects are provided, compositions that exhibit immunity enhancing effects through transdermal absorption when applied in a simple manner or in a patch form have not been provided.

1. Korean Patent Registration No. 10-0815867 2. Korean Patent Registration No. 10-0814214

Accordingly, the technical problem to be solved by the present invention is to provide a composition which is excellent in immunity increasing effect and exhibits immunity increasing effect even through transdermal absorption.

In order to accomplish the above object, the present invention provides a composition for enhancing immunity, which comprises phytoncide, a base oil and azone as an active ingredient.

In the composition for enhancing immunity according to the present invention as described above, the phytoncide may be extracted from pine wood or cottonwood.

In the composition for enhancing immunity according to the present invention, it is preferable that the base oil is any one selected from the group consisting of jojoba oil, coconut oil, and mixtures thereof.

In the composition for enhancing immunity according to the present invention as described above, the phytoncide extract is contained in an amount of 3.0 to 5.0% by weight, the azone is contained in an amount of 0.5 to 2.0% by weight based on the total weight, .

The composition for enhancing immunity according to the present invention as described above preferably further comprises 3.0 to 5.0% by weight of any one selected from the group consisting of hot-water extract of ice plant, hot-water extract of grapefruit, and mixtures thereof.

In the composition for preventing and treating diaper rash according to the present invention, the composition for preventing and treating diaper rash may be a solution, a suspension, an emulsion, a paste, an oil, a spray, a lotion, a coating agent, an emulsion, a cream, Lt; RTI ID = 0.0 > of: < / RTI >

The composition according to the present invention exhibits excellent effects as a high-efficiency immunity enhancer for a normal human with relatively low immunity such as a chronic cold patient through transdermal absorption and also has an excellent effect as an immunity enhancer in anticancer therapy.

Hereinafter, the present invention will be described in detail so as to be easily carried out by those skilled in the art.

The composition for enhancing immunity according to the present invention is characterized by containing phytoncide, a base oil and azone as an active ingredient.

Phytoncide, which is an active ingredient of the present invention, is a compound of phyton, which means plant in Greek, and cide (killer), which means sterilizing power, and means the sterilizing power and natural healing power of plants. Phytoncide contains active ingredients such as terpenes, phenol compounds, alkaloids, and glycosides. It also has an air purification function, a deodorizing action, and activation of the cerebral cortex by surrounding the harmful substances of air such as sterilization power and nitrogen oxides To improve coordination and to take good sleep. In addition, the autonomic nervous stability, liver function improvement, Staphylococcus, Salmonella bacteria and fungi, such as reproductive growth, anti-inflammatory and allergic action, prevention and recovery of allergic diseases, such as the effect of beneficial to the body Is known to be a lot.

The extraction method for producing phytoncide in the present invention is a method widely known in the art to which the present invention pertains, and it is a common knowledge of the technical field to which the present invention belongs, such as steam distillation extraction, compression, volatile solvent extraction, supercritical fluid extraction Any extraction method known to those skilled in the art can be used without any limitation.

The phytoncide according to the present invention may be extracted by the above-described method, but natural phytoncide sold in the market may also be used.

Of the various phytoncides such as those described above, phytoncides suitable for use in the present invention may be those derived from pine wood or cottonwood.

The content of the phytoncide in the composition according to the present invention is preferably 3.0 to 5.0% by weight.

The base oil used as another active ingredient of the present invention is not particularly limited as long as it is a vegetable oil, but it is preferable to use any one selected from the group consisting of jojoba oil, coconut oil, and mixtures thereof.

The above-mentioned jojoba oil is an oil made by extracting seeds of a plant called jojoba, which is large in the desert, and the effect of skin moisturization is known, but the effect on immunity is not disclosed at all.

Coconut oil, which is used as another active ingredient, is extracted from dried coconut milk, and contains lauric acid. Therefore, it has a good moisturizing effect on skin, but its effect on immunity is not known at all.

As the base oil according to the present invention, not only the above-mentioned jojoba and coconut oil but also bergamot, chamomile, geranium, lavender, sandalwood and Frankincense oil can be used.

The extraction method for producing the base oil is a method well known in the art to which the present invention pertains and can be used without any particular limitation as long as it is a method known to those skilled in the art. You can use the oil you sell.

Azone which is used as another active ingredient of the present invention is widely used as a permeation promoter because of its high permeation promoting effect without skin toxicity and is used in a very low concentration of 0.5 ~ 2.0% alone is effective.

That is, the active ingredient of the composition for enhancing immunity according to the present invention is a phytoncide and a base oil, and the azone promotes skin permeation of the active ingredient, thereby improving antibacterial, antiinflammation, general diaper rash improvement and atopic dermatitis So that the effect of improving the oscillation can be remarkably increased.

The composition for enhancing immunity comprising the phytoncide, base oil and azone of the present invention as an active ingredient of the present invention may be prepared in any formulations conventionally prepared for percutaneous absorption, and may be formulated into solutions, suspensions, emulsions, pastes, But are not limited to, oils, sprays, lotions, coatings, emulsions, creams, ointments, patches, and the like.

According to the present invention, the composition comprising phytoncide, base oil and azone as an active ingredient may further comprise any one selected from the group consisting of hot water extract of ice plant, hot water extract of grapefruit, and mixtures thereof And the content thereof is preferably 3.0 to 5.0% by weight based on the total weight of the composition.

The ice plant has blister cells similar to ice crystals attached to stem and leaf surfaces. It is also known as a salty vegetable by sucking salinity from the soil. In addition, it is rich in inositol, pantothenic acid, various minerals, etc., and is effective for antioxidant effect, cholesterol lowering, prevention of diabetes and the like, and is known to be effective for skin health and diet.

Grapefruit is a pale yellow grapefruit rich in juice, tastes strong and has a bitter bitter taste. Grapefruit (grapefruit) is called "grapefruit" because it has an incense similar to grapes and the fruit runs like a cluster of grapes. These grapefruits contain rich vitamin C and lycopene, an antioxidant. Grapefruit is also known to be very beneficial to health because sugar content is low and there are many dietary fiber.

The method for preparing the hot-water extract for these can be used without any particular limitation as long as it is well-known in the art to which the present invention belongs.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to preferred embodiments. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to provide further explanation of the invention as claimed. Of course.

≪ Production of a composition according to the present invention Example >

One. Example  2

200 g of crushed pine wood was washed with purified water and then added to a steam distillation extractor together with 1 L of purified water to conduct steam distillation extraction at 125 ° C for 40 minutes to produce phytoncide.

Base oil was prepared by mixing 100% of natural jojoba seed oil and 100% of organic coconut oil at a weight ratio of 1: 1 to the phytoncide.

Finally, 99% purity azone sold in the market was further mixed to prepare the immunogenic composition according to the present invention.

The content ratio thereof was 3.0% by weight of phytoncide, 1.0% by weight of azone, and the balance weight was base oil.

2. Example  2

200 g of crushed pine wood was washed with purified water and then added to a steam distillation extractor together with 1 L of purified water to conduct steam distillation extraction at 125 ° C for 40 minutes to produce phytoncide.

Base oil was prepared by mixing 100% of natural jojoba seed oil and 100% of organic coconut oil at a weight ratio of 1: 1 to the phytoncide.

Finally, 99% purity azone sold in the market was further mixed to prepare the immunogenic composition according to the present invention.

The content ratio thereof was 5.0% by weight of phytoncide, 1.0% by weight of azone, and the remaining weight% was base oil.

3. Example  3

100 g of the ice plant and 100 g of the grapefruit were boiled in water at 100 ° C for 5 to 6 hours, and then the supernatant liquid was prepared. The composition of Example 2 was further added with 5.0 wt% of hot water extract prepared by taking only the supernatant. Therefore, the content of the base oil is substantially reduced by 5.0% by weight.

< Example  1 to 3 &lt; / RTI &gt; Test Example  1>

Spleen is a major lymphatic organ that collects antigens from the blood and is the primary lymphatic organ that undergoes B and T lymphocyte maturation and stimulation by the antigen, followed by lymphocyte differentiation, which is crucial for the immune system.

40 female Balb / c mice were adapted for a week at 23 ° C, 40-60% humidity, 12 h light-dark cycle conditions.

Thereafter, each of 10 rats was dosed at a dose of 100 g / kg of the composition of Examples 1 to 3, and the rats were administered with a general feed (Purina) for 1 month. Blood was collected, and then a light pigment (FITC, PE) After staining, the number of total T lymphocytes and total B lymphocytes in the collected blood was measured using flow cytometry, and the average value was determined. The results are shown in Table 1.

After the administration of the composition of the examples, only the general diet was administered for 1 month, and blood samples were taken from the control group, and the control group was also 10 mice.

Control group Example 1 Example 2 Example 3 B lymphocyte 11 25 29 38 T lymphocyte 8 20 26 34 * B lymphocytes and T lymphocytes are expressed by cell number X 10 ⁵ .

Table 1 shows the results. It can be confirmed that the compositions of Examples 1 and 2 are excellent in enhancing the immunity since the B lymphocytes and T lymphocytes were significantly increased in the compositions of Examples 1 to 3 according to the present invention compared to the control.

&Lt; Example of preparation of test formulations using the composition prepared according to the present invention & gt ;

Creams containing the composition of the Examples were prepared to test immunity enhancement through percutaneous absorption of the compositions of Examples 1 to 3 above. The cream used in the test was prepared with the composition shown in Table 2 below.

Compounding ingredient
Content (% by weight) Formulation 1 Formulation 2 Formulation 3 Example 1 90.0 - - Example 2 - 90.0 - Example 3 - - 90.0 Carbomer 0.3 0.3 0.3 glycerin 5.0 5.0 5.0 Butylene glycol 2.0 2.0 2.0 Triethanolamine 0.18 0.18 0.18 Disodium iodide 0.02 0.02 0.02 Preservative, incense Suitable amount Suitable amount Suitable amount Purified water Balance Balance Balance

<Immune enhancement effect using the prepared formulation Test Example >

Test Example  1. To normal people Percutaneous  Blood before and after administration Lymphocyte Number change

Changes in the number of lymphocytes in blood before (before) administration of Formulation 1 and after administration (%) were measured and shown in Table 3 below. Table 3 shows that the% lymphocyte content was increased in normal immunocompetent subjects.

The method of administration was such that Formulation 1 of the cream form was administered twice a day, once a day, and once before bedtime, and 2 g was applied uniformly throughout the neck.

patient Before administration After 2 weeks After 4 weeks After 6 weeks Normal range One 27 29 32 35 35-41% 2 29 30 33 36 3 17 25 30 35

Formulations to normal people Percutaneous  Blood before and after administration Lymphocyte Number change

Changes in the number of lymphocytes in blood before (before) administration of Formulation 2 and after administration (%) were measured in normal persons, and are shown in Table 4 below. Table 4 shows that the% lymphocyte content was increased in normal immunocompetent subjects.

In the administration method, Formulation 2 of the cream form was administered twice a day, once a day, and once before bedtime, and 2 g was applied evenly over the entire neck.

patient Before administration After 2 weeks After 4 weeks After 6 weeks Normal range One 26 30 34 38 35-41% 2 28 32 35 40 3 18 25 30 36

Test Example  3. To normal people Percutaneous  Blood before and after administration Lymphocyte Number change

Changes in the number of lymphocytes in blood (before and after administration of Formulation 3) and the changes in blood (unit:%) were measured and shown in Table 5 below. Table 5 shows that the% lymphocyte content was increased in normal immunocompetent subjects.

In the administration method, Formulation 3 of the cream form was administered once a day, twice before bedtime, and 2 g was applied evenly over the entire neck.

patient Before administration After 2 weeks After 4 weeks After 6 weeks Normal range One 27 32 35 39 35-41% 2 29 33 36 40 3 17 30 35 40

Test Example 4. Changes in Blood Lymphocyte Count Before and After Administration to Cancer Patients

The change in lymphocyte count (unit:%) in blood before and after administration of Formulation 1 to cancer patients was measured and shown in Table 6 below. Table 6 shows that the% lymphocyte content was increased in normal immunocompetent subjects.

In the administration method, the above cream form 1 was administered three times a day, one morning, one lunch, one night before sleep, and 2 g was applied evenly over the entire neck.

patient Before administration After 2 weeks After 4 weeks After 6 weeks One 17 21 27 33 2 14 17 24 31 3 21 29 31 35

Test Example  5. Blood before and after administration to cancer patients Lymphocyte  Number change

The change in lymphocyte count (unit:%) in blood before and after administration of Formulation 2 to cancer patients was measured and shown in Table 7 below. Table 7 shows that the% lymphocyte content was increased in normal immunocompetent subjects.

For the administration method, the above cream form 2 was administered three times a day, one morning, one lunch, one night before sleep, and 2 g of the formulation was applied evenly over the entire neck.

patient Before administration After 2 weeks After 4 weeks After 6 weeks One 14 19 27 33 2 15 20 24 31 3 20 28 33 37

Test Example 6. Changes in Blood Lymphocyte Count Before and After Administration to Cancer Patients

The change in lymphocyte count (unit:%) in blood before and after administration of Formulation 3 to cancer patients was measured and shown in Table 8 below. Table 8 shows that the% lymphocyte content was increased in normal immunocompetent subjects.

For the administration method, Formulation 3 of the above cream form was administered three times a day, three times a day, one before lunch, one before sleep, and 2 g was applied evenly over the entire neck.

patient Before administration After 2 weeks After 4 weeks After 6 weeks One 14 26 33 35 2 15 27 32 36 3 21 32 34 39

Through the examples and the test examples described above, it can be confirmed that the composition according to the present invention increases the immunity of patients suffering from chronic cold or chronic fatigue syndrome or cancer patients in a normal person by transdermal administration alone.

Claims (6)

3.0-5.0% by weight of phytoncide extracted from pine wood or cottonwood,
0.5-2.0 wt% of Azone,
3.0 to 5.0% by weight of a mixture of ice plant hydrothermal extract and grapefruit hot water extract; And
Wherein the composition contains at least one of a weight% of a base oil residue selected from the group consisting of jojoba oil, coconut oil, and mixtures thereof.


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