KR101794114B1 - Composition for preventing or treating cancer comprising the extract of Sargassum thunbergii - Google Patents

Abstract

The present invention relates to a composition for preventing or treating cancer containing a marine algae Sargassum thunbergii extract as an active ingredient. The Sargassum thunbergii extract is obtained by undergoing extraction using a solvent mixture of acetone and methylene chloride, and induces apoptosis in U87MG glioblastoma, thereby inhibiting proliferation of cancer cells. Accordingly, the Sargassum thunbergii extract can be applied as a pharmaceutical composition for preventing or treating cancer as well as a health food composition for preventing ameliorating cancer.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing or treating cancer, which contains an extract of Leptospira as an active ingredient,

The present invention relates to a composition for preventing or treating cancer, which contains an extract of Sargassum thunbergii as an active ingredient.

According to the National Statistical Office, deaths from cancer are the number one cause of death among Koreans. Cancer is a disease in which cells are abnormally developed in the cell cycle that constitutes the human tissue and the cells do not normally differentiate and continue to divide. There are three stages of initiation (initiation), promotion, and progression . The cause of cancer is mutation in normal cell gene or cancer suppressor gene by the carcinogenic substance contained in environment or food, and it is known that such cells form cancer tissue by abnormal proliferation while being continuously stimulated by carcinogen, The cause of cancer has not yet been clearly elucidated.

Apoptosis is a vital phenomenon that is essential for normal organ development and tissue homeostasis in multicellular organisms, and refers to the death of spontaneous cells that are induced by a program embedded in the intracellular gene, It is known to cause cancer and degenerative diseases. Therefore, the degree of apoptosis of cancer cells is understood to be an important index of anticancer activity. In addition, cancer is a phenomenon in which normal cell activity is not maintained in cells, growth and division are not controlled, and cells proliferate continuously in an unsteady manner. The degree of such cell cycle control function is also an important index for understanding anticancer activity .

Most of the treatments for cancer are chemotherapy, surgical surgery, radiation therapy, immunotherapy, and the like. Research methods of cancer drugs for treating cancer include a method of searching for direct cytotoxic substances against cancer cells, , A method of searching for a substance that inhibits the metastasis of cancer cells, and a method of searching for a substance that is recently attracting attention as an angiogenesis inhibitor. However, most of the anticancer drugs currently in clinical use are chemically synthesized substances, causing problems such as toxicity to normal cells. In order to solve such problems, recently, a physiologically active substance having anticancer activity derived from natural materials Research is actively underway.

The currently used anticancer drugs can be roughly classified into biological agents such as enzymes and vaccines, chemical synthetic drugs, and natural-derived drugs. Of these, biological agents such as enzymes and vaccines are not in a practical stage, Has a variety of pharmacological actions depending on the type of cancer, and various side effects due to toxicity are pointed out as problems in the treatment of cancer.

Recently, as the cell culture technology has rapidly developed, various cells have been cultured, and various toxic substances have been administered to study the cytotoxic mechanism of these cells at the cell level. In order to increase the effect, development of an anticancer drug using natural products is continuously tried.

Sargassum thumbergii belongs to Phaeopyta mackerel (Sargassaceae). It is a seaweed that is common in the coast of Korea. It lives in the lower part of the intertidal zone, and young plants are used as feeds, but it is a noteworthy resource. However, studies on the physiological activities of the insect worms have shown that antioxidant, anti - inflammatory and antibacterial effects have been demonstrated, and that the substances isolated from the insect worms are (+) - epiloliolide, (-) - loliolide And apo-9'-fucoxanthinone have been reported.

It has been known that it produces a variety of antioxidants in order to cope with damage to tissues and cells due to ultraviolet rays and harsh environments, and it also has various defense mechanisms, such as polysaccharide, . That is, it is expected that functional alcohols having high added value can be developed by utilizing the self-defense component of seaweeds.

Korean Patent Publication No. 10-2010-0131808 (disclosed on December 16, 2010)

It is an object of the present invention to provide a pharmaceutical composition for preventing or treating cancer, which comprises an extract of Seaweed Lobster as an active ingredient.

Another object of the present invention is to provide a health food composition for preventing or ameliorating cancer, which comprises an extract of Seaweed Lobster as an active ingredient.

In order to accomplish the above object, the present invention provides a pharmaceutical composition for preventing or treating cancer, which comprises an extract of Seaweed Lentil as an active ingredient.

The present invention also provides a health food composition for preventing or ameliorating cancer, which comprises an extract of Seaweed Lobster as an active ingredient.

The present invention relates to a composition containing an extract of seaweed as an active ingredient, and more particularly to a composition for preventing or treating cancer by inducing the death of the extract of the insect such as cancer cells.

In addition, the above-mentioned insomnia extract can be used as a health food for cancer prevention or improvement.

FIG. 1 is a graph showing the cell survival rate of U87MG human glioblastoma caused by algae extract. The optical density at 490 nm was measured, and the cell viability was compared with that of the control by a histogram.

The inventors of the present invention produced an extract of seaweed larvae using a mixed solvent of acetone and methylene chloride, and confirmed the inhibitory activity of cancer cell proliferation by inducing apoptosis of U87MG glioblastoma, confirmed the possibility as a pharmaceutical and health food composition, .

As used herein, the term " Sargassum thunbergii " refers to algae belonging to the family Phaeophyta (Sargassaceae) and commonly found in the coastal waters of Korea, and various antioxidants are produced in-house. And a variety of defense mechanisms, such as creating a plant to carry out life activities.

The term " prophylactic " as used herein means all actions by which the insect inhibits cancer or delays its development by the extract.

As used herein, the term " treatment " refers to any action by which the worm causes the symptoms of cancer to be improved or benefited by the extract.

The present invention provides a pharmaceutical composition for preventing or treating cancer, which comprises an extract of Seaweed Lentil as an active ingredient. In detail, the extract of the seaweed lipid can inhibit the proliferation of cancer cells by inducing apoptosis.

Preferably, the seaweed extract can be extracted with a mixed solvent of acetone and methylene chloride.

Preferably, the acetone and the methylene chloride may be mixed in a volume ratio of 1: 1.

Preferably, the cancer is selected from the group consisting of brain cancer, esophageal cancer, stomach cancer, liver cancer, pancreatic cancer, kidney cancer, small bowel cancer, colon cancer, rectal cancer, lung cancer, breast cancer, ovarian cancer, ovarian cancer and prostate cancer. .

When the composition of the present invention is a pharmaceutical composition, it may contain a pharmaceutically acceptable carrier in addition to the above-mentioned active ingredients. Such pharmaceutically acceptable carriers are those conventionally used in pharmaceutical preparations, and include lactose, dextrose, The present invention relates to a process for the preparation of a medicament for the treatment and / or prophylaxis of cancer, comprising administering a therapeutically effective amount of a compound selected from the group consisting of cross, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, Carboxymethylcellulose, carboxymethylcellulose, hydroxybenzoate, talc, magnesium stearate, mineral oil, and the like. The pharmaceutical composition may further contain a filler, a weight agent, a binder, a lubricant, a wetting agent, a disintegrant, a sweetener, a flavoring agent, an emulsifying agent, a suspending agent, a fragrance, a preservative and the like as an additive and an auxiliary agent.

The pharmaceutical composition may be formulated into tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, nonaqueous solutions, emulsions, lyophilized preparations, suppositories and sterile injectable solutions. The pharmaceutical composition of the present invention may be administered orally or parenterally such as intravenously, intraarterally, intraperitoneally, intramuscularly, intrasternally, subcutaneously, intradermally, intranasally, intrapulmonary, rectally, topically, ≪ / RTI > can be administered in a conventional manner.

The method of administration of the pharmaceutical composition is determined depending on the severity of symptoms, and local administration is generally preferred. The effective amount of the active ingredient of the above pharmaceutical composition means an amount required for prevention or treatment of the disease. Accordingly, the present invention is not limited to the particular type of the disease, the severity of the disease, the kind and amount of the active ingredient and other ingredients contained in the composition, the type of formulation and the patient's age, body weight, general health status, sex and diet, Rate of administration, duration of treatment, concurrent medication, and the like. But is not limited thereto.

The present invention provides a health food composition for preventing and ameliorating cancer, which contains an extract of Seaweed Lobster as an active ingredient.

Preferably, the seaweed extract can be extracted with a mixed solvent of acetone and methylene chloride.

The health food composition may be provided in the form of powder, granules, tablets, capsules, syrups or beverages. The health food composition may be used in combination with other food or food additives other than the active ingredient, . The amount of the active ingredient to be mixed can be suitably determined according to its use purpose, for example, prevention, health or therapeutic treatment.

The effective dose of the active ingredient contained in the above health food composition may be used in accordance with the effective dose of the pharmaceutical composition, but in the case of long-term consumption intended for health and hygiene purposes or health control purposes, And it is clear that the active ingredient can be used in an amount exceeding the above range since there is no problem in terms of safety.

There is no particular limitation on the kind of the above health food and examples thereof include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen and other noodles, gums, ice cream, Drinks, alcoholic beverages and vitamin complexes.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example  1: Preparation of algae extract

A total of 23 samples were collected from 14 species of seaweed, Arctic seaweed, Antarctic seaweed, and domestic iso-species seaweed, which were collected at different sampling times, sampling sites, and sampling depths. A total of 23 samples were washed, lyophilized at -20 ° C, and homogenized in a fine powder form using a pulverizer. 1 g of each algae sample in the powder state was extracted with a mixed solvent of acetone and methylene chloride in the same volume ratio (1: 1) for 24 hours, and this procedure was repeated twice.

For use as a control, 1 g of each algae sample was extracted with the same procedure as above using a single methanol solvent. The obtained extracts were concentrated using a rotary evaporator at 40 ° C in a vacuum state. The final sample was dissolved in dimethylsulfoxide (DMSO) immediately before use in the experiment.

sample Scientific name GG2 Grateloupia lanceolata GG3 Grateloupia lanceolata GG4 Grateloupia lanceolata GG5 Grateloupia lanceolata GG6-1 Ecklonia stolonifera GG6-2 Ecklonia stolonifera GG7 Ecklonia stolonifera GG10 Laminaria  japonica GG11 Laminaria  japonica GG12 Porphyra seriata GG16 Ulva pertusa GG17 Codium  fragile GG18 Laminaria  japonica GG19 Gymnogongrus flabelliformis GG20 Chondrus ocellatus GG21 Grateloupia lanceolata GG22 Grateloupia okamurai GG23 Chondria crassicaulis GG24 Porphyra yezoensis GG27 Grateloupia lanceolata GG30 Sargassum thunbergii GG34 Ecklonia cava GG36 Ulva pertusa

Example  2: Seaweed A witch  Inhibitory effect of extract on cell survival

In order to confirm the inhibitory activity of cancer cell proliferation by algae extract, U87MG (human glioblastoma) was used. U87MG is a glioblastoma, also referred to as a pontine ganglion, and refers to a ganglionally derived tumor of the brain that constitutes the brain. Each algae extract was treated with 100 mg / ml of U87MG and cultured for 24 hours. Each algae sample is shown in Table 1 above. The cell viability was measured by optical density at 490 nm using MTT assay, and the cell viability compared with the control was expressed by histogram.

As a result, referring to FIG. 1, 23 seaweed samples extracted with a single methanol solvent showed no effect on the U87MG survival rate. On the other hand, the survival rate of U87MG was decreased in some seaweed extracts of 23 algae samples extracted with mixed solvents of acetone and methylene chloride at the same volume ratio (1: 1). Among them, GG30 sample showed the best induction of cancer cell death , And the GG30 sample was found to be an extract of Lepidoptera.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (9)

A pharmaceutical composition for preventing or treating brain cancer, which comprises an extract of Sargassum thunbergii as an active ingredient and extracting the extract with a mixed solvent of acetone and methylene chloride. [Claim 3] The pharmaceutical composition for preventing or treating brain cancer according to claim 1, wherein the extract of Seaweed Lentil has an inhibitory activity against cancer cell proliferation. delete The pharmaceutical composition for preventing or treating brain cancer according to claim 1, wherein the acetone and the methylene chloride are mixed at a volume ratio of 1: 1. delete The pharmaceutical composition according to claim 1, wherein the pharmaceutical composition is in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, nonaqueous solutions, emulsions, lyophilized preparations, Wherein the pharmaceutical composition for preventing or treating brain cancer has one of the following formulations. A health food composition for preventing or ameliorating brain cancer characterized by containing an extract of Sargassum thunbergii as an active ingredient and extracting the extract with a mixed solvent of acetone and methylene chloride. delete The health food composition according to claim 7, wherein the health food composition is selected from the group consisting of dairy products including soups, sausages, breads, chocolates, candies, snacks, confections, pizzas, ramen noodles, gums, ice creams, Wherein the composition is one selected from the group consisting of an alcoholic beverage and a vitamin complex.

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