KR101785271B1 - Methods of Preventing Plant Diseases by Using Composition Comprising the Extract of Amorpha fruticosa - Google Patents

Abstract

The present invention relates to an antifungal substance including an extract of Amorpha fruticosa and a method for controlling plant diseases using the same. The present invention can be used for the development of a plant disease control agent for an antimicrobial activity-effective bioprocess. According to the present invention, the extract of Amorpha fruticosa is derived from a natural product and is harmless to the human body and does not cause environmental pollution, and can be used for plant fungal diseases such as barley powdery mildew, wheat red rust, tomato blight and tomato gray mold And thus can be usefully used for the development of environmentally friendly natural product disinfectants and the production of high value added organic agricultural products.

Description

(Methods of Preventing Plant Diseases by Using Composition Comprising the Extract of Amorpha fruticosa)

The present invention relates to a method of controlling plant diseases using an antifungal agent comprising an extract of Amorpha fruticosa .

Shao et al. Were isolated protein having an antifungal effect from seeds of Phytolacca americana, and the cDNA was cloned into the amino acid sequence of this protein (Biochim Biophys Acta 1999 Mar 19; 1430 (2):.. 262-8) Kobayashi et Phyolacca Americana Kaluso and Botrytis fabae were double-cultured to produce antifungal substances (Z Naturforsch C. 1995 May-Jun; 50 (5-6): 398-402.)

In particular, Cladosporium herbarum showed a very high antifungal effect and found to be phytolaccoside B (Pls B) as a main component. And the like succeeded in separating the antifungal substance Kaepferol-3-O-β-D-glucopyranoside from the leaves of Phytolacca americana . This material showed high antimicrobial activity against various plant pathogens such as Botrytis cinerea, Magnaporthe grisea, Penicillium italicum, Diaporthe actinidiae, Botryosphaeria dothidea and Colletotrichum gloeosporioides, and inhibition of fungal growth by cell wall dissolution was a cycle of antimicrobial activity Respectively. Borchardt et al. Reported the antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans from water-soluble ethanol extracts from the stem, leaf, roots and flowers of natural or domesticized species separated from Minnesota and Wisconsin. Here, we have found that Amorpha canesecns has an antibacterial effect against Staphylococcus aureus . Samie et al. Reported that Amorpha fruticosa has an antifungal effect against Cryptococcus neoformans (African Journal of Biotechnology Vol. 9 (20), pp. 2965-2976, 17 May, 2010).

However, there has been no report of antifungal agents against plant pathogens from Amorpha fruitcosa . The inventors succeeded in isolating the fungicidal material from Amorpha fruitcosa and completed the present invention.

The object of the present invention is to provide a method for controlling a plant disease by using an antifungal substance isolated from Amopha fruitcosa .

A first aspect of the present invention relates to a method for preparing Amorpha fruticosa ; Extracting the plant with an organic solvent; Purifying the extract; And treating the purified extract with a plant that has been infected by the mold with a mold.

Preferably, the organic solvent is a C ₁ to C ₆ lower alcohol.

More preferably, the organic solvent is methanol. The plant disease can be selected from the group consisting of blast disease, rice sheath stain disease, tomato gray mold, tomato blight, and anthracnose anthracnose. The plant disease can be selected from the group consisting of Magnaporthe oryzae, Rhizoctonia solani, Botrytis cinerea, Phytophthora infestans and Colletotrichum coccodes May be any one or more plant diseases caused by any one or more fungi selected from the group.

The features and advantages of the present invention are summarized as follows:

(i) Antimicrobial activity The present invention can be used for the development of a plant disease control agent for biological resources.

(ii) According to the present invention, the extract of Amorpha fruticosa is derived from a natural product and is harmless to the human body and does not cause environmental pollution, and can be used for the treatment of rice blast, rice sheath stain, tomato gray mold, And thus can be usefully used as an environmentally friendly natural product disinfectant.

Figure 1 shows the dose-dependent inhibitory activity of Amorpha fruticosa methanol extract in inhibitory activity. A, whole plant after the onset of infection on day 7 of infection indicating pepper anthracnose (PAN). Plant extracts were sprayed on the 1st day before inoculation of the pathogen. 1, pathogen without plant extract; 2, 750 ppm plant extracts and pathogens; 3, 1,500 ppm plant extracts and pathogens; 4, 3,000 ppm plant extracts and pathogens; 5, no pathogen. B. Dose-dependent inhibition values of A. fruticosa methanol extract against pepper anthracnose (PAN).

Hereinafter, the present invention will be described in more detail by way of examples. However, these examples are merely illustrative of the present invention and the scope of the present invention is not limited to these examples.

Materials and Methods

Target plant disease

Target plant disease is rice blast: pathogen Magnaporthe oryzae , rice sheath blight: pathogen Rhizoctonia solani , tomato gray mold: pathogens Botrytis cinerea , tomato lateblight: Pathogen Phytophthora infestans , wheat leaf rust: Puccinia recondita , barley powdery mildew: pathogen Blumeria graminis f. sp. hordei , pepper anthracnose: The pathogen Colletotrichum coccodes .

Chemical solution treatment

Rice (5th leaf stage), tomato (2nd leaf stage), wheat (1st leaf stage) and barley (1st leaf stage) were used for 1 ~ 4 weeks in greenhouse. Three pot plants per crop bottle were placed on a rotating plate and sprayed with the solution prepared in the previous step.

Inoculation and onset

Rice blast: Spore suspension of 1x10 ⁶ spores / ml was sprayed on the treated rice and cultivated for 4 days in a constant temperature and humidity room after wet treatment for 1 day in wet condition.

Rice sheath stain bottle: Mycelia cultivated in bran medium were ground with a Waring blender and put on a rice pot, cultivated for 4 days in a greenhouse after wet treatment for 3 days in wet condition.

Tomato gray mold disease was induced by inoculating a spore suspension of ⁵ × 10 ⁵ spores / ml prepared on the treated tomatoes for 3 days in a wet condition.

Spore suspension (1 × 10 ⁴ sporangia / ml) was sprayed on tomatoes treated with low temperature treatment and spawning suspension (1 × 10 ⁴ sporangia / ml) from spawning pellets. The seedlings were inoculated for 2 days in wet condition and cultured in a constant temperature and humidity room for 1 day.

Wheat rust: The wheat rust bacillus subsp. Was added to Tween 20 solution (250 / ml) and shaken well to prepare a spore suspension. The inoculated wheat was cultivated for 6 days in a temperature and humidity chamber after a wet treatment for 1 day.

Red pepper anthracnose: Spores of C. coccodes formed on the medium were harvested and Tween 20 was added thereto to prepare a spore suspension having a spore concentration of 3 × 10 ⁵ spores / ml and a Tween 20 concentration of 100 / ml. Respectively. The inoculated pepper seedlings were treated for 2 days and then cultivated for 1 day in a constant temperature and humidity chamber to induce anthrax to pepper leaves.

Disease investigation and control

Bottleneath area of each crop was examined after 3 ~ 7 days of inoculation. (%) = (1 - lesion area ratio of treatment / lesion area ratio of untreated lesion) x 100

Results of plant pathogen activity test

The control effects on 7 kinds of phytopathogenic fungi are shown in Fig. 1 and Table 1. Amorpha fruticosa was effective against rice blast, rice sheath stain, tomato gray mold, tomato blight and red pepper anthracnose. However, wheat rust and barley powdery mildew were not effective.

[Table 1]

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (5)

The method comprising treating a C ₁ to C ₆ lower alcohol extract of Amorpha fruticosa with at least one plant disease-induced plant selected from the group consisting of rice sheath stain disease, tomato blight, and red pepper anthracnose Disease control method.
delete delete The plant disease control method according to claim 1, wherein the plant disease is caused by one or more fungi selected from the group consisting of Rhizoctonia solani, Phytophthora infestans and Colletotrichum coccodes . delete

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