KR101785149B1 - insecticide method - Google Patents
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- KR101785149B1 KR101785149B1 KR1020150082114A KR20150082114A KR101785149B1 KR 101785149 B1 KR101785149 B1 KR 101785149B1 KR 1020150082114 A KR1020150082114 A KR 1020150082114A KR 20150082114 A KR20150082114 A KR 20150082114A KR 101785149 B1 KR101785149 B1 KR 101785149B1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/06—Blanching
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S424/00—Drug, bio-affecting and body treating compositions
- Y10S424/10—Insect repellent
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- Y10S514/919—
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- Pest Control & Pesticides (AREA)
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Abstract
The present invention relates to a insecticidal method, characterized in that a subject grain is treated with chlorine dioxide and heat treatment in parallel with the method of insect insect inhabiting the grain.
Description
The present invention relates to insecticidal methods.
The study on Korean pest insects as a low pest was conducted at the National Agricultural Products Inspection Office (1993), and the National Institute of Agricultural Science and Technology (1996) prepared 70 pest insects. And ecology. The most common types of pests that occur in the actual distribution stage are pseudo rice thistles and moths moths, which are caused by rice and rice. In particular, the pest insects that occur are rice moths except for moths and barley moths The weevil, the false rice thistle, and the broad-leaved mite are very small in size and less than 3 mm in size, which is a pest that requires careful observation.
Post-harvest pest management techniques are largely divided into chemical and physical treatments. Chemical treatments include fumigation treatment, such as methyl bromide or phosphine, that penetrates deep into crops, and treatment of saponification substances or chemical pesticides to remove pests that settle on the surface. On the other hand, physical treatment methods include temperature (high temperature and low temperature) treatment, environmental conditioning treatment, radiation treatment, and mixing treatment thereof. The temperature treatment is a control technique using the survival limit range against the temperature of the insect pest. The environmental control treatment means a temperature treatment in which a high concentration of carbon dioxide and a low concentration of oxygen are combined. Irradiation causes the disinfection effect by disrupting the chemical bonding of the pest DNA. Other physical treatments include ozone treatment, microwave treatment, radio frequency heating, high pressure oxygen treatment and vacuum treatment. As the import of agricultural products abroad is expected to increase sharply due to the signing of a free trade agreement between global nations, exports of domestic agricultural products are also competitive. Methyl bromide is currently being processed, but this will gradually be prohibited as ozone depleting substances And alternative technology development is urgently required.
On the other hand, the inventor of the present invention has disclosed the insecticidal effect of chlorine dioxide in the above-mentioned study, and, in spite of excellent insecticidal effect of chlorine dioxide, when chlorine dioxide is used to kill insect pests of grain, insect evasion behavior is observed , Thus greatly reducing the effectiveness of chlorine dioxide fumigation. Therefore, it is necessary to develop a technology that can suppress such evasive action and utilize chlorine dioxide in actual grain insect pests.
Korean Patent Laid-Open No. 2001-0029166 discloses an invention relating to pulp bleaching method of chlorine dioxide, but there is no research on the insecticidal method using chlorine dioxide and the avoidance behavior of insects.
It is an object of the present invention to provide a method for efficiently insecting eggs, larvae, pupa, adults, and the like of insects in grains.
The present invention provides a method of insect control for insects inhabiting grain, characterized in that the subject grain is treated with chlorine dioxide and heat treatment is performed in parallel.
The present invention is to maximize the insecticidal effect of insects in grains caused by chlorine dioxide by inhibiting the action of avoiding insects against chlorine dioxide. In the case of heat treatment mixing treatment in chlorine dioxide treatment, the insecticidal effect is maximized, The effect of the sole treatment of chlorine dioxide and the effect of the mixing treatment are remarkably superior to those of each case.
1 is a graph showing chlorine dioxide (12 h exposure) evaluated against a false rice thistle ( T. castaneum ). Figure 1a shows the treatment concentration-toxicity curve in the false rice thistle larvae and adults. Figure 1B shows susceptibility to false rice thistle larvae and adults. Each evaluation was performed in 3 replicates for 15 individuals. The different markers on the standard deviation bar represent a significant difference between the means at the Type I = 0.05 (LSD test).
FIG. 2 is a graph showing a reduction in toxicity of chlorine dioxide (200 ppm for 24 h) to a false rice thistle ( T. castaneum ) with increasing food. Each evaluation was performed in 3 replicates for 15 individuals in a 50 mL tube. The horizontal axis "Diet depth" of the graph represents the volume of food in the test tube. 5 mL is equivalent to a feed depth of about 2 cm. The different markers on the standard deviation bar represent a significant difference between the means at the Type I = 0.05 (LSD test).
Figure 3 shows a test for behavior of a false rice thief ( T. castaneum ) with chlorine dioxide (ClO 2 ) using a Y tube. 3A is a schematic view of a Y tube. (C) (control), T (test chemical: ClO 2 ), L C is the control line, L T is the test chemical limit line, NR is the limit line of the no response, Evaluated using.)
FIG. 3B shows the effect of antennas on directional behavior of food in the Y tube (+ ANT (with whiskers) and -ANT (without whiskers)). Each evaluation was repeated three times.
Figure 3c shows the effect of chlorine dioxide on the preference behavior of the respondent. Each evaluation was repeated three times.
Fig. 4 shows the kinetic values according to chlorine dioxide treatment for a false rice thistle ( T. castaneum ) adult. 4A is a schematic diagram of the experimental equipment, and "X" represents a target insect. Figure 4b shows the walking distance for 5 minutes. 4C shows the gait speed. Each evaluation was repeated 20 times. The different markers on the standard deviation bar represent a significant difference between the means at the Type I = 0.05 (LSD test).
Figure 5 shows the synergistic effects of chlorine dioxide and heat (46 < 0 > C) treatment on false rice thistle ( T. castaneum ) adults. Each evaluation was repeated 20 times. The different markers on the standard deviation bar represent a significant difference between the means at the Type I = 0.05 (LSD test).
The present invention relates to a method of insect insect inhabiting grains, characterized in that the subject grain is treated with chlorine dioxide and heat treatment in parallel. The inventors of the present invention have found out that chlorine dioxide has excellent insecticidal effect and this chlorine dioxide has a very excellent effect on insecticide. However, when this chlorine dioxide treatment method is applied for the actual insecticidal action , It is experimentally confirmed that there is a problem that it is difficult to obtain sufficient insecticidal effect due to the avoidance behavior of insects by chlorine dioxide. In addition, when the chlorine dioxide treatment is performed at a relatively low temperature in combination with the heat treatment, it is confirmed that the insecticidal action by the chlorine dioxide is maximized by suppressing the evasive action of the insects, thus completing the present invention.
The insect may be a pest insect, may be a false rice thistle ( T. castaneum ) or an angelic moth, and may be one or more selected from eggs, larvae, pupae, and adults of a specimen of a false rice thistle have.
In addition, the insecticide method may be performed in a closed object space. The target space may be, but is not limited to, a warehouse where agricultural products are stored or grown, a container box, a plastic container with a hole, a plastic container with a bag or a lid, and a bag.
The chlorine dioxide may be in a gaseous state or in a liquid state sprayed by spraying. The liquid sprayed by the spray method may be a mist-like liquid.
Accordingly, chlorine dioxide can be attached to the surface of the object in the object space.
The chlorine dioxide may be treated at a concentration of 0.1 ppm to 800 ppm and preferably at a concentration of 1 ppm to 800 ppm. The chlorine dioxide is preferably treated to the object for 1 to 72 hours, Depending on the type of insecticide, the treatment concentration and treatment time can be controlled.
For example, it is possible to completely kill chlorine dioxide by treating 100 ppm X 24 hours with chlorine dioxide, so that 2400 ppm / hour is a complete kill condition. In addition, there is an effect of killing the insecticidal object even under these lower conditions.
The heat treatment may preferably be performed at 30 to 50 占 폚. Within this temperature range, it is possible to maximize the killing effect of insects by chlorine dioxide by preventing evasive action against the chlorine dioxide of insects, while minimizing damage to the quality of the grain by heat.
Hereinafter, the present invention will be described in more detail based on examples. However, the embodiments of the present invention described below are for illustrating the present invention, and the scope of the present invention is not limited to these embodiments. The scope of the invention is indicated by the appended claims, and includes all changes within the meaning and range of equivalency of the claims.
Reference Example :
(One) A false rice thief multiplication
(100 g), wheat germ (50 g) and yeast extract (15 g) were mixed with each other. The breeding container was filled with about 5 cm of food in a circular plastic container with a diameter of 15 cm, and all breeding periods were mixed and bred. The upper surface of the breasts was covered with mesh for air circulation. The food was exchanged about every 3-4 weeks. The larvae used in the test were 5 mm or more in length. Adults were selected for the test within 10 days after allegation. All insects were bred at 25 ℃ and 60% relative humidity.
(2) Y tube behavior analysis
The glass tube of Y tube had an internal diameter of 3 cm, the length of both arms was 16 cm and the angle of 45 ° was extended to each other, and the length of the common tube was 13 cm (see FIG. 3 a). Nitrogen gas is connected to both arms and designed to escape through the common pipe. Nitrogen gas first allowed water to enter both arms of the Y tube through a half-filled bottle to contain water vapor. The rate of injected nitrogen gas was set at 300 mL / min. 500 mg of dummy rice thistle feed was applied to the ends of both arms, and 100 μL of chlorine dioxide solution (800 ppm) was treated with phosphate buffer solution and control solution, respectively. False - paddy rice thieves to be treated were fasted for 24 hours, placed on a common pipe base, and moved for 10 minutes. At this time, the individuals moving 3 cm from the starting point to the food were defined as the responding individuals. The individuals moving to one side past the branching point determined the individuals moving 24 cm from the branching point as the preferred ones. After the treatment, the Y tube was washed with 95.5% acetone and again with 99% hexane. Finally, rinsed with water, dried and used in the next experiment.
(3) Analysis of gait behavior
Walking distance and walking speed after exposure to the chlorine dioxide of the false rice thieving larvae were analyzed by an insect behavior meter (EthoVision XT, Version 9.0, Noldus Information Technology, Wageningen, Netherland). The behavioral stadium was a Petri dish of 55 mm in diameter. A 1.5-mL E-tube lid was placed in the center of the stadium, and 200 μL of chlorine dioxide (800 ppm) liquid or phosphate buffer solution was placed thereon. The whole behavior was observed for 5 minutes, and each treatment was repeated with 30 individuals. During the treatment period, the temperature was 25 ° C and the relative humidity was 40%.
(4) Chlorine dioxide fumigation
Chlorine dioxide was produced by electrolysis. Since it was dissolved in water and fumed in gas form, it was possible to control the concentration. It was administered to a sealed chamber specially made of acrylic plate using a gas generator (Pureo Farm, Hwaseong City, Korea). Test insects were administered with a small amount (approximately 10) of brown rice in a 50 mL tube. The upper surface of the vessel was covered with mesh to enable fumigation treatment. This insect container was placed in a chlorine dioxide treatment chamber and the concentration of chlorine dioxide was continuously monitored with the gas leak detector C16 (Analytical technology, Collegeville, PA, USA) during the treatment period to confirm the treatment concentration.
(5) For chlorine dioxide A false rice thief Susceptibility analysis
The chlorine dioxide concentration treatment was 0, 100, 200, 400 and 800 ppm. Each concentration treatment was exposed for 3 hours in 3 replicates. After the treatment, the rats were allowed to stand at 25 ℃ for 72 hours. Larvae and adult deaths were determined to have no arbitrary behavior when lightly pressing the abdomen with tweezers. The death determination of the pupa was defined as no movement of the abdomen when the chest was held with tweezers.
(6) Heat treatment and compound treatment
Heat treatment was carried out at 46 ℃ using a constant temperature water bath. Seedlings were treated in 15 mL of 50 mL tubes filled with 10 mL of prey. The water in the constant-temperature water bath was treated to reach the lid portion of the tube. The lid was replaced with mesh. The constant temperature water bath was transferred into the chlorine dioxide fumigation chamber and the untreated and 400 ppm chlorine dioxide treatment was carried out for 6 hours and 12 hours, respectively. Under the same conditions, chlorine dioxide alone treatment was carried out simultaneously inside the chlorine dioxide treatment chamber outside the constant temperature bath. Each treatment was carried out in 3 replicates.
Example One: False rice thief in a dick Toxicity assessment of chlorine dioxide
The susceptibility of the false rice thistle to the chlorine dioxide fumigant treatment according to the method of the above reference example was analyzed and the results are shown in Fig. 1 (Fig. 1A). Chlorine Dioxide to False Rice Throat showed significant toxicity depending on treatment concentration (F = 196.10; df = 4,22; P <0.0001). The increased toxicity of these chlorine dioxide concentrations was similar for both larvae and adults ( F = 0.06; df = 4, 22; P = 0.3083). However, the susceptibility to chlorine dioxide was different depending on the development stage (Fig. 1b), indicating that the pupa was more sensitive than the larvae and adults ( F = 0.54; df = 2, 22; P <0.0001). (LC 50 ) of 382.67 ppm (153.63 - 955.78 ppm: 95% confidence interval) for adult larvae and 397.75 ppm (354.46 - 446.13 ppm: 95% confidence interval) for adult larvae, Respectively.
Example 2: Confirmation of chlorine dioxide toxicity deterioration due to avoidance behavior
When the flour feed was treated in various volumes, the susceptibility of the false rice thistle to chlorine dioxide was greatly reduced by the avoidance behavior of both larvae and adults (Fig. 2). This decrease in susceptibility was prominent in proportion to the fed amount ( F = 49.0; df = 7, 32; P <0.0001). This tendency was similar in larvae and adults ( F = 0.82; df = 7, 32; P = 0.5773). Especially, when the amount of food was 5 mL or more (about 2 cm or more in depth), almost no treatment effect was shown.
A Y tube bioassay was conducted to analyze whether a false rice thief was avoiding chlorine dioxide (Fig. 3). Insects were fed to both ends of the Y tube (Fig. 3A). The control was treated with 800 ppm chlorine dioxide and the control was treated with the same volume of phosphate buffer solution. In this case, 82% of the false rice thieves had behavioral responses to the food movement (Fig. 3b). However, when the tactile sense was removed, about 77% of the subjects were unresponsive. Of the normal individuals that responded to food, about 88% moved toward the untreated chlorine dioxide (Figure 3c). However, most of the tactile deceased rice thieves lost their ability to detect food, and some responding individuals were biased toward chlorine dioxide treatment and lost their ability to detect chlorine dioxide compared to the control ( X 2 = 35.6; df = 2; P < 0.0001). These avoidance behaviors are also supported by increased walking speed and distance with chlorine dioxide treatment (Fig. 4).
Example 3: Assessment of chlorine dioxide toxicity recovery by heat treatment and mixing
The lowering of the susceptibility of the false rice thistle to chlorine dioxide was observed to be due to the behavior of avoiding chlorine dioxide exposure while digging into the food. In order to reduce this avoidance behavior, heat treatment was performed in parallel (Fig. 5). In heat treatment alone, it showed 4.4% insecticidal activity at 6 hours treatment and 93.3% insecticidal power at 12 hours treatment. However, treatment with chlorine dioxide at 400 ppm did not show any insecticidal activity in both treatment. However, the combination of the two treatments showed a significant synergistic effect ( F = 160.82; df = 2, 12; P <0.0001) in the 6 hour treatment with 93.3 percent insecticidal activity. And the two treatments which were treated for 12 hours showed complete control effect.
Claims (6)
The heat treatment is carried out at a temperature of not lower than 30 DEG C to lower than 50 DEG C;
Wherein said insecticidal method is to inhibit the insect's avoiding behavior against chlorine dioxide by said heat treatment;
Wherein said insect is an egg, larva, pupa and adult of a false rice thistle (T.castaneum).
Wherein the chlorine dioxide has a concentration of from 0.1 ppm to 800 ppm.
Wherein the chlorine dioxide treatment and the heat treatment are performed for 1 to 72 hours.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2012108480A1 (en) | 2011-02-10 | 2012-08-16 | 大幸薬品株式会社 | Insecticide and insecticidal method |
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| KR100327392B1 (en) | 1999-09-29 | 2002-03-13 | 윤병호 | The Method of Chlorine Dioxide Pulp Bleaching using additive |
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| WO2012108480A1 (en) | 2011-02-10 | 2012-08-16 | 大幸薬品株式会社 | Insecticide and insecticidal method |
Non-Patent Citations (3)
| Title |
|---|
| Infection Control and Hospital Epidemiology(2012.05.) 저널의 vol.33, no.5* |
| Journal of Stored Products Research 39(2003) 저널의 p555-569* |
| 한국식품영양과학회지(2008) 저널의 vol.37, p1337-1342 |
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