KR101776919B1 - Manufacturing method of organic liquid fertilizer using ascidian tunic and organic liquid fertilizer thereby - Google Patents

Abstract

The present invention relates to a method for producing a fermented broth by fermenting a raw material mixed with an ash shell powder, a beneficial microorganism (EM), molasses and water, and aging the fermented broth, and a fermented broth fermented by the fermented broth, Is a fermentation product obtained by fermenting a useful component of a fermented bean curd into a specific kind of beneficial microorganism and a specific amount of molasses, and aging the fermented beef to thereby extract an ingredient useful in fertilizer from the ascid peel in an optimum state A fermentation broth production method, and a fermentation broth fermentation broth produced thereby.
Also, since the fermentation broth is produced using ascook peel, beneficial microorganism and molasses as the main raw material, the present invention can provide the effect that the produced fermentation broth ratio does not contain various harmful bacteria harmful to crops; It is possible to acquire as much as possible the three major elements of fertilizer such as nitrogen, phosphorus and potassium and free amino acid, which are contained in the ascension peel, which is a byproduct waste after machining or eating asciton, which is one of marine organisms. Can be obtained; Particularly, it is possible to obtain an effect that the whole of the ascid shell in a hard state can be fermented and aged to be recycled as the fermentation broth.

Description

[0001] The present invention relates to a fermentation broth containing an ascid shell and a fermented broth containing the same,

The present invention relates to a method for producing a fermented broth by fermenting a raw material mixed with an ash shell powder, a beneficial microorganism (EM), molasses and water, and aging the fermented broth, and a fermented broth fermented by the fermented broth, Is a fermentation broth obtained by mixing a useful fertilizer component present in the ascid bark with a specific kind of beneficial microorganism and a specific amount of molasses to ferment and aging the fermented product to extract an ingredient useful in fertilizer from the ascid peel in an optimum state. A non-production method, and an ascook peel fermentation liquid ratio produced thereby.

In environmentally friendly crop cultivation, the main crops are mainly used as greaves. In the subspecies, the crops are easily available when the crops are nutrient deficient. The ratio of microbial fermentation broth not only has a direct effect on the growth of crops, but also a secondary effect produced by microbial degradation of various organic matter as well as nutrient supply effect. , The utilization value of microbial fermentation broth is very high in eco-friendly agriculture.

Ascidian is a fish food source with high palatability due to its reproductive characteristics. The production amount is increasing every year along with the development of aquaculture technology. Most of the consumption form of icy crab is mostly used for reproductive purposes, so that most of the shell part is abandoned. Has become a problem of coastal environmental pollution.

The ascid shell, which is a waste resource, is composed of hard stone cells which are difficult to decompose, but it contains a large amount of carotenoid pigment components, inorganic components such as Ca, P and Mg, and a large amount of protein and taurine. It is possible to develop it as a comprehensive nutritional supplement containing various components necessary for the nutritional supplement.

Studies on the asparagus bark have been reported on studies on food ingredients, dietary polysaccharide with various physiological activities, feed additive for poultry and poultry using pigment and enzyme hydrolyzate (Kim, 2002) There is no research data on the application of fertilizer. Especially, researches on growth regulators that promote the growth of crops have not been conducted at all.

Recently, it has been required to study the optimum fermentation conditions of livestock shells and the characteristics of livestock using the beneficial microorganism (EM) for the development of environmentally friendly organic livestock using an ascid shell, which is a waste resource.

The present invention relates to a method for manufacturing fermentation broth by conventional fermentation broth, and a method for manufacturing a fermentation broth according to the related art is a fermentation process for fermenting a raw material such as food waste or by- However, the fermentation broth prepared as described above has a problem in that various kinds of harmful bacteria are proliferated and adversely affect crops.

Despite the fact that the fermentation broth prepared using the byproduct waste produced after the processing or ingestion of various kinds of marine organisms is obtained by fermenting the components useful for the various fertilizers contained in each marine organism by an appropriate method, There was a problem of obtaining only useful components;

Particularly, since a large amount of the ascid bark, which is produced after processing or ingesting the aspen, is hard, the problem of not being used at all due to the fermentation broth has arisen.

The present invention has been made to solve the above-

A raw material mixing step of mixing 10 to 30% by weight of dried and pulverized ascid peel powder, 1 to 10% by weight of useful microorganisms (EM), 5 to 25% by weight of molasses and 40 to 70% by weight of water to prepare a mixed raw material; Fermenting the mixed raw material in a fermenter for 15 to 25 days to produce a fermented product; Aging the fermented product for aging for 15 to 25 days to produce aged product; And a filtration step of filtering the aged product to obtain an ascook peel fermentation broth, and a fermentation broth using the ascid shell and a fermented broiler fermentation broth.

As described above, the fermentation broth production method using the ascid peel according to the present invention produces the fermentation broth ratio by using ascook peel, beneficial microorganism (EM) and molasses as the main raw materials. Therefore, various kinds of harmful bacteria An effect not included can be obtained;

It is possible to acquire as much as possible the three major elements of fertilizer such as nitrogen, phosphorus and potassium and free amino acid which are contained in the ascension peel which is a byproduct waste which is produced after processing or eating the ascension which is one of the marine organisms Can be obtained;

Particularly, it is possible to obtain an effect that the whole of the ascid shell in a hard state can be fermented and aged to be recycled as the fermentation broth.

The present invention relates to a method for producing a fermentation broth by fermenting a raw material mixed with an ash shell powder, a useful microorganism, molasses and water and aging the fermented broth, and a fermented broth fermented by the fermentation, A raw material mixing step of mixing 10 to 30% by weight of powder, 1 to 10% by weight of useful microorganisms (EM), 5 to 25% by weight of molasses and 40 to 70% by weight of water to prepare a mixed raw material; Fermenting the mixed raw material in a fermenter for 15 to 25 days to produce a fermented product; Aging the fermented product for aging for 15 to 25 days to produce aged product; And a filtration step of filtering the aged product to obtain an ascook peel fermentation broth ratio, and a fermentation broth using the ascid shell and a fermentation broth fermented by the fermentation broth.

First, the ascid peel according to the present invention is a by-product of marine organisms generally known as "sea squirt", which is a by-product obtained after obtaining the meat quality of the crab as processing or consuming, hard and difficult to eat, It is difficult to use as a general fertilizer.

Specifically, the raw material mixing step is performed by mixing 10 to 30% by weight of pulverized ascook peel powder, 1 to 10% by weight of useful microorganism (EM), 5 to 25% by weight of molasses and 40 to 70% It is a process of mixing ascook peel, beneficial microorganism, molasses and water made of powder.

That is, the ash shell powder can be prepared in various ways as long as it is ground to a predetermined size by drying the hardened ascid shell. However, the washed ascid shell is dried at a temperature of 45 to 70 ° C for 3 to 7 days, A drying process to make the shell; And a pulverizing step of pulverizing the dried ascic peel to an average particle size of 40 to 60 meshes.

At this time, the drying process is preferably a drying process before powdering the ascid peel, and the drying process is preferably performed at a relatively low temperature of 45 to 70 DEG C for 3 to 7 days.

That is, when the drying process temperature is less than 45 ° C, the drying process takes too long, and when the drying temperature exceeds 70 ° C, some organic components useful for the fertilizer contained in the umbrella shell may be destroyed. It is preferable that the drying process treatment temperature is treated within the above temperature range.

The pulverization process is a process of pulverizing the dried ascid peel to an average particle size of 40 to 60 meshes. When the pulverized ascid peel is crushed to a size larger than 40 mesh, the pulverized ascid peel powder is too large, And the fermentation period takes a long time. When the powder is crushed to a size smaller than 60 mesh, the size of the powder is too small, which makes it difficult to manage the ash shell powder. Therefore, the ash shell powder is pulverized .

In addition, it is preferable that the ash shell powder is mixed at a composition ratio of 10 to 30 wt% in the mixed raw material. When the ash shell powder is less than 10 wt%, the composition of the ash shell powder is insufficient, The content of the ash shell powder is excessively high. When the content of the ash shell powder is more than 30% by weight, the extraction efficiency of the useful ingredient in the fertilizer by the ash shell is remarkably decreased. Is preferably contained in the mixed raw material at a composition ratio within the above range.

In addition, useful microorganisms (EM, effective microorganisms) are a mixture of lactic acid bacteria and yeast, and general useful microorganisms can be used. Lactobacillus plantarum, Lactobacillus casei, It is more preferable to mix Saccharomyces cerevisiae.

In addition, since the specific gravity of each composition is different from that of the mixed raw materials in which useful microorganisms are mixed, it is preferable to shake or stir the mixture so as not to bubble during mixing. If bubbles are generated at this time, oxygen is present in a large amount in the mixed raw material, and the probability of corruption increases rather than fermentation.

In addition, it is preferable that the useful microorganisms are mixed in a composition ratio of 1 to 10% by weight to the mixed raw materials. When the useful microorganism is mixed in less than 1% by weight, fermentation of the ash shell powder by the useful microorganisms does not occur, The fermentation step needs to be treated for a long period of time. If the content exceeds 10% by weight, the composition of the useful microorganism is excessive, and the fermentation efficiency of the ascension peel is decreased, Is preferably contained in the mixed raw material at a composition ratio within the above range.

In addition, molasses is a constituent used as a substrate for beneficial microorganisms. The molasses is preferentially used as a feed of yeast contained in useful microorganisms to activate the activity of yeast, and subsequently acts as a food of lactic acid bacteria contained in useful microorganisms. Activate. As described above, the yeast and lactic acid bacteria are activated and fermentation of the ash shell powder starts.

That is, since the molasses determines the degree of activation of the useful microorganisms and determines the degree of fermentation of the ash shell powder by the activated microorganism and the degree of extraction of the fertilizer-utilizing component in the fermented asparagus bark, The molar ratio of molasses is very important.

In connection with the above, in the present invention, it is preferable that the molasses is mixed with the mixed raw material at a composition ratio of 5 to 25% by weight, and a detailed description thereof will be made using the following examples.

In addition, the water to be mixed in the mixed raw materials is included in the other composition ratios except for the ash shell powder, useful microorganisms and molasses contained in the mixed raw materials, and is preferably included in the mixed raw materials in a composition ratio of 40 to 70% by weight.

Also, the fermentation step is a process for fermenting the mixed raw material in a fermentation tank for 15 to 25 days to make a fermented product, and fermenting the mixed raw material to make a fermented product.

That is, the fermentation step can be carried out by various methods as long as the microorganism useful for the fertilizer contained in the ash shell powder is fermented by the fermentation of the ascension peel powder, and the composition of the mixed raw material is the fermented product for 15 to 25 days .

At this time, if the treatment period of the fermentation step is less than 15 days, the fermentation of the ascic peel powder is insufficient and the extraction effect of the useful ingredient is insufficient. When the fermentation step is carried out for more than 25 days, The effect is excellent, but some other useful components are rather reduced, and the treatment period of the fermentation step is preferably within 15 to 25 days.

Preferably, the fermentation step is performed in an anaerobic fermenter maintaining an anaerobic state at a temperature of 32 to 38 ° C.

Specifically, the anaerobic microorganism group contained in the useful microorganisms contained in the mixed raw material is preferably fermented in the anaerobic fermentation tank because it is preferable that the anaerobic microorganism group is fermented in a state where oxygen is blocked. In the upper part of the anaerobic fermentation tank, It is preferable that a large amount of gas generated due to respiration of the anaerobic microorganism group during the fermentation process is discharged to the outside of the anaerobic fermenter.

If the temperature of the anaerobic fermenter is lower than 32 ° C, the fermentation does not progress smoothly due to insufficient activation of the lactic acid bacteria among the useful microorganisms due to the low temperature. If the temperature exceeds 38 ° C, acidic pulp may occur due to rapid fermentation. It is desirable that the temperature is maintained at 32 to 38 ° C.

The aging step is a step of aging the fermented product produced in the fermentation step for 15 to 25 days to prepare a fermented product, and stabilizing useful components of the fermented product fermented by the useful microorganism.

At this time, the period of the aging step may be varied for various periods of time according to the judgment of a person skilled in the art, but it is preferable to treat for 15 to 25 days. If the aging step is less than 15 days, the aging period is too short, And if it exceeds 25 days, the process time of the aging step is excessively long and the productivity is lowered. Therefore, it is preferable that the aging step treatment period is processed within the above range.

In addition, although the above-mentioned aging step treatment temperature can be freely set according to the judgment of a person skilled in the art, it is preferable that the useful microorganism contained in the fermentation product is treated at a temperature of 13-20 ° C so as to be no longer activated.

At this time, if the aging temperature is less than 13 ° C, the aging temperature is too low to stabilize the fermentation product to slow down the economical efficiency. If the temperature exceeds 20 ° C, some useful microorganisms may be activated and the fermentation product may be corrupted. The aging step is preferably carried out at a temperature of 13 to 20 ° C.

The filtration step is a step of filtering the aged product produced by the aging step to obtain an ascook peel fermentation broth ratio. The aging product is filtered using a filtration apparatus equipped with a general filter net, It can be processed by collecting obesity of fermentation liquid of liquid ascook peel.

The following is a preferred test example of the fermentation broth production method using the ascid peel according to the present invention and the result of analyzing the components useful for the fertilizer contained in the prepared fermentation broth.

[exam]

1. Samples and Pretreatment

The asparagus bark, which is a non-raw material of the fermentation broth used in this Example, was produced from the offshore of Kyongnam Tongyeong, and the sugarcane molasses was a product (spring) which was declared as an eco-friendly farming material. The useful microorganisms used in the fermentation were lactic acid bacteria (lactobacillus plantarum, lactobacillus casei) and yeast (saccharomyces cereviseae) were used. The ascid shells were washed with tap water and then hot-air dried at 60 ° C for 4 days, finely crushed to a particle size of 50 mesh using a roll mill and a cut mill, and stored at room temperature.

Ascite peel, which is a non-raw material of fermentation broth used in this example, had 8.51% moisture and 6.23 g ㆍ 100 g ^-1 of total nitrogen and the highest amount of calcium was 2.80 g ㆍ 100 g ^{- 1} among cations (Table 1). Also molasses was 20.05% moisture, fertilizer three elements from potassium is 2.40g and 100g ^-1, cations in the calcium and 2.80g 100g ^- was the highest content of ^1.

2. Sample input ratio and culture conditions

20% by weight of ascook husks and 5% by weight of useful microorganisms (EM) were added to the mixed raw materials, and then 5% by weight, 10% by weight, 15% by weight and 20% by weight of molasses of sugarcane molasses Conditions were selected (Table 2).

The fermentation broth was prepared by anaerobically fermenting the aerobic fermenter (20L) equipped with an external air inflow preventing device capable of discharging gas at the upper end by well mixing 10 kg of the material according to the input conditions, sealing it in a culture chamber set at 36 ° C, 14 days, 21 days, 28 days, and 50 days, the changes of the fermentation broth according to the culture period were repeated three times. In order to prevent contact with oxygen during sampling, a valve was installed at the bottom of the fermenter to collect samples.

In addition, the above sampling is a sample obtained before the aging step for the accurate analysis of the effective ingredient of the fertilizer which occurs during the fermentation process of the ascension peel.

3. Analysis method of sample

Analysis of the ratio of fermentation broth of ascid peel was analyzed by referring to the method of quality inspection of fertilizer (RDA, 2006) notified by RDA.

The pH and electrical conductivity (EC) of the fermentation broth were obtained by collecting 100 mL of each sample. 2 filter paper, and then measured using a pH meter (Mettler, MP 220) and an EC meter (TOA CM-40S). Total nitrogen was measured by Kjeldahl method after wet decomposition with sulfuric acid. Phosphoric acid, substitutional cations and trace elements were analyzed by ICP (Varian 720-ES). Free amino acid analysis of Ascid peel liquor was carried out by centrifuging the sample (10 mL) at 15,000 rpm for 15 minutes, filtering the supernatant through a 0.45 μm membrane filter, and measuring it with an amino acid automatic analyzer (L-8900, Hitachi, Japan). The mobile phase solvent used in the amino acid autoanalyzer was lithium citrate buffer, the flow rate was 0.35 mL / min, and 20 μL of the sample was injected into the analyzer. The temperature of the column and the reactor were measured at 50 ° C and 135 ° C, respectively. The ninhydrin reagent was used as the reactant solution, and the absorbance was measured at 570 nm and 440 nm.

[Test results and discussion]

1. Usefulness of fermentation broth of ascidian husk pH and EC (electrical conductivity) during fermentation of microorganism (EM)

 Table 3 shows changes in pH and EC according to the fermentation period of the ingredients according to the input ratio of the asparaginous shell liquor.

The pH of the material was decreased in the order of T1, T2, T3 and T4. During the fermentation, the pH of the fermented starch showed a value of 4.76 at the 5th day of fermentation at T1 containing 5% by weight of molasses, but gradually increased with the elapse of time, showing 6.75 at the fermentation at 50th day of fermentation. The pH value after 5th day of fermentation tended to be kept constant in the experimental group.

In case of fermentation using beneficial microorganism (EM), organic acid is generated in the course of decomposition of organic matter by lactic acid bacteria present in EM, and the pH tends to be kept low. Therefore, EM fermentation seems to proceed well in the T2, T3 and T4 test sites where 10% or more molasses was added.

The EC of fermented asperity peel fermented with EM increased in the order of T1, T2, T3, and T4, and increased with increasing molasses input. As the fermentation progressed, the EC value gradually increased at the 28th day of fermentation, and then decreased slightly after the fermentation.

In the case of T4, which has the highest molasses input ratio, it gradually increased up to 50 days of fermentation. These results suggest that ions are dissociated from organic matter into solution due to the mineralization of organic matter by microorganisms during the fermentation process.

2. Changes of T-N, P and K during fermentation of useful microorganism (EM) fermentation broth of Ascid peel

Table 4 shows the T-N, P and K contents of the fermentation broth of ascook peel according to the fermentation period of each ingredient.

The total nitrogen content of the fermentation broth of ascidian husk fermented by EM fermentation was significantly increased with the fermentation period of all fermentation period. %, While that of T2 increased by 118.5%, T3 increased by 219.7% and T4 increased by 218.7 times, indicating that the total nitrogen content increased by 15% by weight molasses. In addition, total nitrogen content of each test group was increased by 174.5% in T2, 229.4% in T3, and 344.1% in T4 compared with T1 in the 50th day of fermentation.

The phosphorus content of the fermentation broth of ascidian bark was increased by EM fermentation until 21 days of fermentation in all treatments, but decreased rapidly thereafter. The change of phosphorus content according to fermentation period was decreased by 40.3% in T1 and 31.2% in T2 at the 50th day of fermentation compared to the fermentation at 21st day, while T3 was decreased by 10.9% and T4 was decreased by 8.0% And it was found that the reduction rate was reduced more effectively when the molasses was added in an amount of 15 wt% or more. In comparison with T1, the contents of T2, T3 and T4 were increased by 176.7%, 271.1% and 341.9%, respectively, on the 21th day of fermentation with the highest phosphorus content.

Potassium content of fermented asperity peel juice increased gradually with the fermentation period of fermentation from 5th day to 50th day of fermentation, and the increase of T1 was 7.5% in T1 and 4.5% in T2 On the other hand, T3 and T4 increased 26.5% and 39.4%, respectively, indicating that the addition of more than 15% by weight of molasses resulted in an increase in the potassium content. In the fermentation on the 50th day of fermentation, the content of potassium in each test was 187.3% in T2, 235.6% in T3, and 334.7% in T4 compared with T1.

3. Usefulness of fermentation broth of asparagus bark (fermentation period)

Table 5 shows changes in contents of calcium, magnesium, and sodium, which are the main inorganic components of the ascook peel, according to the fermentation period for each ingredient input ratio.

The content of calcium in the fermentation broth of asparagine peel increased with fermentation period. The increase rate from 5th day to 50th day of fermentation showed 13%, 5.7%, 26.5% and 39.8% %, Respectively. In the fermentation on the 5th day of fermentation, the content of calcium in each test was higher than that of T1, 202.6% in T2, 210.3% in T3, 271.2% in T4, and 189.6% in T2, 235.6% in T3, Was 334.7% higher than that of fermentation.

The content of magnesium in fermentation broth of ascidian was gradually increased with the fermentation period, and the growth rate from the 5th day to the 50th day of fermentation was 3.3%, 9.0%, 22.6% and 27.4% And the addition rate of molasses was more than 15% by weight. In comparison with T1, the content of magnesium was 158.5% in T2, 189.3% in T3, 235.3% in T4, and 167.1% in T2, 224.6% in T3 and T4 in fermentation on the 5th day of fermentation Was 290.1% higher than that of fermentation. It was found that the difference of magnesium content in the test section was larger than that of calcium fermentation at the 50th day after fermentation. The content of magnesium in the fermentation period was about half that of calcium.

Sodium content of fermented asperity peel fermentation broth was highest at 28 days of fermentation at T1 and T2, but decreased slightly at fermentation period of T3 and T4. From the 5th day of fermentation to the 50th day of fermentation, the rate of increase of sodium was 13.7% in T1, 11.3% in T2, 6.0% in T3 and 11.5% in T4. The sodium content of the fermentation broth of Aspergillus oryzae was largely eluted at the early stage of fermentation and the difference was small during fermentation period.

4. Free amino acid content during fermentation period of useful microorganism (EM) of fermentation broth of ascidian shell

The total amount of free amino acids was found to be 7,080.94 mg / L in the free amino acid analysis of the fermented liquid of fermented ascook peel, which was fermented by EM for 50 days. Respectively.

Among the free amino acids, alanine, which promotes the sweetness and coloration of fruit, showed the highest content of 656.32 mg / L. As amino acids of silkworm, - Aminobutyric acid (GABA), aspartic acid which improves soil solubility and sugar content, leucine which has the effect of improving the color of fruits, glycine which increases the sugar content and increase the cold resistance showed an amino acid content of 500 mg / L or more, Respectively. Followed by valine, serine, ornithine, phenylalanine, lysine, proline, tyrosine and glutamic acid in the order of 469.36-207.24 mg / L, and threonine, isoleucine and taurine. Methionine and cystathionine showed the contents of 189.98-111.58 mg / L.

As a result, the fermentation broth using the ascid peel according to the present invention showed that the pH was lowered and the EC was increased as the molasses input was increased in the production of fermentation broth using the ascid peel. The pH was higher than 10 wt% It remained constant in the test area.

The total nitrogen content of fermentation broth using ascook peel increased significantly as fermentation progressed. In particular, the fermentation broth showed an increase rate of about 220% in the fermented broth containing more than 15% by weight of molasses. The content of phosphorus was the highest at 21 days of fermentation, and the content of potassium increased with fermentation.

The content of calcium, magnesium and sodium in the fermentation broth using the ascook peel also increased with fermentation, and the content of calcium was higher than that of the fermented broth containing 15 wt% or more of molasses.

In addition, total 29 free amino acids of ascidic peel liquor were detected, total amount was 7,080.94 mg / L and alanine was the highest content of 656.32 mg / L. Based on the above results, molasses may be contained in the mixed raw material in an amount of 5 to 25% by weight in order to make livestock using the ascid shell, and it is considered that the condition in which molasses is added in an amount of 15% by weight or more is optimal.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It is possible to carry out various changes in the present invention.

Claims (14)

A raw material mixing step of mixing 10 to 30% by weight of dried and pulverized ascid peel powder, 1 to 10% by weight of useful microorganisms (EM), 5 to 25% by weight of molasses and 40 to 70% by weight of water to prepare a mixed raw material;
Fermenting the mixed raw material in a fermenter for 15 to 25 days to produce a fermented product;
Aging the fermented product for aging for 15 to 25 days to produce aged product;
And filtering the aged material to obtain an ascook peel fermentation liquid ratio,
The ash shell powder in the raw material mixing step is prepared by drying the washed ascid shell at a temperature of 45 to 70 캜 for 3 to 7 days to form a dried ascid peel; And a pulverizing step of pulverizing the dried ascic peel to an average particle size of 40 to 60 meshes,
The beneficial microorganism (EM) is obtained by mixing lactic acid bacteria Lactobacillus plantarum, Lactobacillus casei and Saccharomyces cerevisiae, which is a yeast, A method for producing a fermentation broth using a fermentation broth.
delete delete The method according to claim 1,
Wherein the fermentation step comprises:
In an anaerobic fermenter maintaining the anaerobic condition, at a temperature of 32 to 38 DEG C,
The aging step is configured to be treated at a temperature of 13 to 20 DEG C,
The fermented ascook peel fermentation broth has a pH of 4.21 to 4.99,
To be an electrical conductivity (EC) of 19.09 to 28.50 dS / m,
A total nitrogen content of 2,225 to 8,825 mg / L,
And a phosphorus content of 923 to 3,305 mg / L,
With a potassium content of 2,007 to 6,533 mg / L,
A calcium content of 2,036 to 6,590 mg / L,
With a magnesium content of 1,00 to 2,7978 mg / L,
And a sodium content of 1,089 to 2,537 mg / L. delete delete delete delete delete delete delete delete delete delete